CN105420297A - Fermentation method for preparing soapberry saponin extract - Google Patents
Fermentation method for preparing soapberry saponin extract Download PDFInfo
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- CN105420297A CN105420297A CN201510987596.3A CN201510987596A CN105420297A CN 105420297 A CN105420297 A CN 105420297A CN 201510987596 A CN201510987596 A CN 201510987596A CN 105420297 A CN105420297 A CN 105420297A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/56—Lactic acid
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Abstract
The invention provides a fermentation method for preparing a soapberry saponin extract. The method comprises the steps that enzymatic saccharification is performed on a soapberry extract, and then inoculated fermentation is performed through lactic acid bacteria. The invention further provides a fermented refined product of the soapberry saponin extract. According to the characteristic that a soapberry pericarp extract contains higher-concentration cellobiose and mannose, the method comprises the steps that enzymatic saccharification and fermentation are performed on the soapberry pericarp extract, carbohydrate and a small amount of protein in the soapberry pericarp extract are converted into lactic acid, therefore, soapberry pericarp is fully utilized, the compound product of soapberry saponin and the lactic acid is obtained, and high-value utilization of soapberry fruit is achieved. According to the method, interference impurities such as carbohydrate and protein in an aqueous soapberry extract solution are directly converted into effective components in the final product, and the prepared refined soapberry liquid product is stable in quality and high in purity and can be used for preparing liquid detergent or other liquid product.
Description
Technical field
The invention belongs to field of natural product extraction, relate in particular to a kind of method that fermentation method prepares sapindust saponin extract.
Background technology
Natural saponin, also known as saponin, can form the aqueous solution or colloidal solution and the plant glucosides that can form saponaceous foam is referred to as, and is made up of sapogenin and sugared, uronic acid or other organic acids.According to the molecular structure of known sapogenin, saponin(e can be divided into two large classes, a class is steroidal saponin, and another kind of is triterpenoid saponin.Saponin(e mostly is white or oyster white amorphous powder, and minority is crystal, and bitter and pungent is irritant to mucous membrane.The general water soluble of saponin(e, methyl alcohol and Diluted Alcohol, be soluble in hot water, hot methanol and hot ethanol, is insoluble to ether, chloroform and benzene.Saponin(e is very strong tensio-active agent, even if high dilution also can form soap lye.Saponin(e has hormesis to heart; It is again very strong hemolytic agent.Often be present in foxglove, Chinese squill and some leguminous crops.Because saponin contains a large amount of triterpenoid saponins, having higher whipability, in daily use chemicals industry, is rare natural surface active agent, has developed that saponin shampoo draws, saponin bathing is clean at present, and washing effect is all better than like product.Due to the biological activity of saponin, in medical, also there is very large application, can as antitumor, immunity moderation power, the medicine of cardiovascular and cerebrovascular diseases.
Seed of Chinese Soapberry (SapindusmukurossiGaertn.) has another name called wood and suffers from sub, oily trouble, bitter trouble tree, yellow order tree etc., belonging to Sapindaceae (Sapindaceae), is fallen leaves megaphanerophyte, Gao Keda more than 20 rice, bark beige or chocolate, be mainly distributed in east China, south to the west and south.The ground such as Japan, Korea, South East Asia Mainland and India are also often cultivated.Seed is spherical, black, hard.Root and fruit are used as medicine, and have function that is clearing heat and detoxicating, preventing phlegm from forming and stopping coughing; Containing saponin(e in pericarp, soap can be replaced to be used as to wash, to be especially suitable for the washing of silk quality product; Wood quality is soft, sapwood yellow-white, and heartwood tawny can do boxboard and wooden comb etc.
Containing 20% sapindust saponin of having an appointment in Seed of Chinese Soapberry shell, there are some researches show, this kind of saponin is a kind of natural nonionogenic tenside, has good lathering property, and its surfactant is triterpene saponin and sesquiterpene glucosides class, fatty oil and protein.Effectively can remove dirt, good cleaning capacity, and report in some documents sapindust saponin have antibacterial, brighten, nti-freckle, anti-acne, prevent dermopathic medicinal function, can as the major ingredient of natural active matter in various skin care product.
Lactic acid (Lacticacid) has another name called alpha-hydroxypropionic acid, and its molecular formula is C
3h
6o
3, structural formula CH
3cHOHCOOH, its relative molecular weight is 90.08, is a kind of organic acid extensively existed in animal, plant and microorganism.Lactic acid has an asymmetric C atom and has opticity on molecular structure, can be divided into: left-handed (D-), dextrorotation (L-) and racemization (DL-) by opticity.Lactic acid is usually in viscous liquid shape, and color is colourless or light yellow, has tart flavour and fatty acid odor slightly.Be divided into industry, food and pharmacopeia grade according to purity lactic acid, its purity is respectively 50 ~ 90%, more than 80% and 85 ~ 90%.Under vacuum condition (67 ~ 133Pa), repeatedly fractionation can obtain white lactic acid crystallization, and the fusing point of sterling lactic acid is 122 DEG C, and boiling point is 168 DEG C.Lactic acid is naturally present in skin, is therefore widely used as skin care product wetting Agent for Printing Inks and moisturizing agent.Lactic acid be in hair intrinsic, hair can be made to be full of gloss, to be therefore often used as the pH adjusting agent in treatment.Lactic acid, as pH adjusting agent and wetting Agent for Printing Inks, can be used in bath wash supplies.
Containing abundant saponin and glucide in Seed of Chinese Soapberry Aqueous extracts, needing further separation and purification, a large amount of foams can be produced when carrying out vacuum evaporation process, cause operating unit to be difficult to carry out continuously; When Aqueous extracts direct effect liquid washing agent or pesticide synergistic agent, again owing to containing abundant glucide in Aqueous extracts, cause product stability poor; It is high that the drying of Aqueous extracts Direct spraying is prepared into energy consumption needed for dry powder, and in dry powder, product foreign matter content is high simultaneously.
Although the purposes of the saponin in Seed of Chinese Soapberry pericarp is well known, to its refining blank being still this area with the method obtaining the excellent liquid product of result of use.
Summary of the invention
According to the cellobiose containing higher concentration in Seed of Chinese Soapberry Aqueous extracts and seminose, the present invention proposes one with Seed of Chinese Soapberry Aqueous extracts for substrate, fermented by enzymatic saccharification, glucide in Aqueous extracts and a small amount of albumen are converted into the lactic acid that can be used for daily chemical products and use, thus obtain the composite product of sapindust saponin and lactic acid, for preparing Seed of Chinese Soapberry liquid washing agent or other liquid products provide feasible efficient technological line.
For the deficiencies in the prior art, the object of the invention is to propose a kind of method that fermentation method prepares sapindust saponin extract.
Another object of the present invention proposes to ferment the product obtained by described method.
The technical scheme realizing above-mentioned purpose is:
A fermentation process for sapindust saponin extract, comprises step: by soapberry extract enzymatic saccharification, then ferments with lactobacillus inoculum.
Wherein, described soapberry extract, after being pulverized by Seed of Chinese Soapberry pericarp, uses flooding products therefrom.
Wherein, described pulverizing is that Seed of Chinese Soapberry fruit peel powder is broken to below 1mm, and described flooding is at 50-70 DEG C of temperature, adds Seed of Chinese Soapberry pericarp quality 5-7 water doubly, lixiviate 2-5h.
Wherein, described enzymatic saccharification be add in cellobiase, mannase and polygalacturonase in soapberry extract one or more carry out enzymatic saccharification.
Wherein, described enzymatic saccharification be to pH value be add in the soapberry extract of 4.6-5.0 that consumption is the cellobiase of 8-12IU, one or more in the polygalacturonase of the mannase of 15-25IU or 3-4IU carry out enzymatic saccharification, the time of enzymatic saccharification is 80-100h, and temperature is 30-50 DEG C.
Wherein, described lactobacillus inoculum fermentation is under pH value 6.8-7.5 condition, and inoculating lactic acid bacterium quality is the 0.4-0.6% of enzymatic saccharification product quality.
Wherein, add minimal medium or yeast extract medium during lactobacillus inoculum fermentation, described minimal medium consists of: MgSO
47H
2o0.4-0.6g/L, KH
2pO
30.4-0.6g/L, NaCl0.09-0.11g/L; Described yeast extract medium consists of: MgSO
47H
2o0.4-0.6g/L, KH
2pO
30.4-0.6g/L, NaCl0.09-0.11g/L, yeast extract paste 5g/L.
Wherein, the temperature of described lactobacillus inoculum fermentation is 40-48 DEG C, and fermentation time is 140-160h.Add calcium carbonate during fermentation, make fermented liquid final ph control at 5.5-7.0, better ferment effect can be obtained.
Wherein, also comprise the step of the rear solid-liquid separation of lactobacillus inoculum fermentation, get the product liquid after separation.
The tunning that fermentation process of the present invention obtains.
Beneficial effect of the present invention is:
1) according to the feature containing higher concentration cellobiose and seminose in Seed of Chinese Soapberry peel extract, Seed of Chinese Soapberry peel extract is proposed to ferment through enzymatic saccharification, glucide wherein and a small amount of albumen are changed into lactic acid, thus make full use of Seed of Chinese Soapberry pericarp, obtain the composite product of sapindust saponin and lactic acid, realize the higher value application of Seed of Chinese Soapberry fruit.
2) because sapindust saponin and lactic acid all as fungistat, and all can be able to add in daily chemical products, as skin care articles for washing, this compound product produces one matter work simplification more separately, and product yield is high.
3) with Seed of Chinese Soapberry water extraction saponin solution fermenting lactic acid compared with traditional fermenting lactic acid production technique, have that reaction conditions gentleness, side reaction are little, raw material availability high, and single step reaction can prepare saponin and lactic acid two series products simultaneously.
4) this process for refining eliminates evaporation or drying unit, and energy expenditure reduces greatly.
5) do not contain glucide in sapindust saponin Purified Water extract, constant product quality, purity is high, can be used for preparing liquid washing agent or other liquid products.
6) the interference impurity such as the sugar in Seed of Chinese Soapberry Aqueous extracts, albumen are converted into the active principle in the finished product, collection product purification and fermentation reaction one, technique is created.
Embodiment
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.
The milk-acid bacteria added in embodiment is thermophilic lacto-bacilli, purchased from Harbin Meihua Biologic Technology Co., Ltd..
Cellobiase is purchased from Novozymes Company.Mannase is purchased from Novozymes Company.Polygalacturonase available from Sigma.
Embodiment 1
By air-dry Seed of Chinese Soapberry pericarp (moisture content≤12%) impurity elimination deironing, irony is may be mixed in collection process, and it has detrimentally affect to disintegrating apparatus.Pulverize pericarp with electric crusher, pulverize as 1mm and following fragment, add the water of 5 times of its quality, at 60 DEG C, mechanical shaking extraction saponin in the constant-temperature table of 150 turns, 4h.Extracting solution being placed at rotating speed is in the whizzer of 3000rpm, centrifugal 15min, collects supernatant liquid, is Seed of Chinese Soapberry Aqueous extracts.By the NaOH solution of 10%, it is 4.8 that dropping 1-2 drips regulator solution pH value.Add 10IU cellobiase to solution, 45 DEG C, in the full temperature concussion incubator of 180rpm, carry out saccharification 96h.
30%NaOH solution regulator solution pH value is 7.0.Solution is divided into 4 parts, numbering 1,2,3,4.In No. 1 group, add yeast extract medium, do not access milk-acid bacteria, do not add CaCO3; In No. 2 groups, do not add substratum, add milk-acid bacteria; In No. 3 groups, add minimal medium, CaCO3 and 0.5% milk-acid bacteria, in No. 4 groups, add yeast extract medium, 0.5% milk-acid bacteria and CaCO3,4 sample sets are put into 42 DEG C, in the full temperature concussion incubator of 130rpm, carry out fermentation 144h.
First sterilizing before substratum adds: sterilising conditions is 121 DEG C, under the condition of 0.1MP, sterilizing 15min, adds after cooling in Bechtop.Sugared concentration and lactic acid concn in fermented liquid is detected with high performance liquid chromatography.The rotational rate of lactic acid of No. 1 group is 16.09%, and final fermented liquid pH value is 7.91; The rotational rate of lactic acid of No. 2 groups is 69.92%, and final fermented liquid pH value is 6.88; The rotational rate of lactic acid of No. 3 groups is 76.66%, and final fermented liquid pH value is 6.54; The rotational rate of lactic acid of No. 4 groups is 88.42%, and final fermented liquid pH value is 6.02; Glucose concn 0g/L, mannose concentration 0g/L in fermented liquid, remain without monose.
1-4 experimental result shows, does not access milk-acid bacteria or does not add substratum, all can reach certain ferment effect when fermentation.But access milk-acid bacteria, add yeast extract medium and calcium carbonate, better ferment effect can be reached.The half of the total reducing sugar that enzymolysis produces is determined by liquid chromatogram measuring result.
Embodiment 2:
By air-dry Seed of Chinese Soapberry pericarp impurity elimination deironing, electric crusher pulverizes pericarp, crosses 20 mesh sieves, adds the water of 6 times of its quality, at 70 DEG C, and mechanical shaking extraction saponin in the constant-temperature table of 150rpm, 2h.Extracting solution being placed at rotating speed is in the whizzer of 3000rpm, centrifugal 15min, collects supernatant liquid, is the Aqueous extracts of sapindust saponin.Be 4.8 by the NaOH solution regulator solution pH value of 10%.Add 10IU cellobiase, 20IU mannase and 0.2mg/ml polygalacturonase to solution, 50 DEG C, in the full temperature concussion incubator of 180rpm, carry out saccharification 80h.30%NaOH solution regulator solution pH value is 7.5.Add in enzymolysis solution the yeast extract medium after sterilizing (in fermentating liquid volume, MgSO47H2O0.4g/L, KH2PO30.4g/L, NaCl0.09g/L, yeast extract paste 5g/L), milk-acid bacteria 0.4%(mass ratio) and CaCO3, at 48 DEG C, in the full temperature concussion incubator of 130rpm, carry out fermentation 140h.Glucose concn and lactic acid concn in fermented liquid is detected with high performance liquid chromatography.Rotational rate of lactic acid 79.59%, final fermented liquid pH value is 6.81; Glucose concn 0g/L, mannose concentration 0g/L in fermented liquid, remain without monose.
Adopt the solid-liquid two-phase of the whizzer separate fermentation gained suspension of 4000rpm, liquid phase is the composite solution of sapindust saponin and lactic acid.
Embodiment 3:
By air-dry Seed of Chinese Soapberry pericarp impurity elimination deironing, electric crusher pulverizes pericarp, crosses 20 mesh sieves, adds the water of 6 times of its quality, at 50 DEG C, and mechanical shaking extraction saponin in the constant-temperature table of 150rpm, 5h.Extracting solution being placed at rotating speed is in the whizzer of 3000rpm, centrifugal 15min, collects supernatant liquid, is the Aqueous extracts of sapindust saponin.Be 5.0 by NaOH solution regulator solution pH value.Add 8IU cellobiase, 15IU mannase and 0.2mg/ml polygalacturonase to solution, 50 DEG C, in the full temperature concussion incubator of 180rpm, carry out saccharification 100h.30%NaOH solution regulator solution pH value is 7.5.Add in enzymolysis solution the yeast extract medium after sterilizing (in fermentating liquid volume, MgSO
47H
2o0.6g/L, KH
2pO
30.6g/L, NaCl0.11g/L, yeast extract paste 5g/L), milk-acid bacteria 0.6%(mass ratio) and CaCO
3, at 40 DEG C, in the full temperature concussion incubator of 130rpm, carry out fermentation 160h.Glucose concn and lactic acid concn in fermented liquid is detected with high performance liquid chromatography.Rotational rate of lactic acid 79.59%, final fermented liquid pH value is 6.83; Glucose concn 0g/L, mannose concentration 0g/L in fermented liquid, remain without monose.
Adopt the solid-liquid two-phase of the whizzer separate fermentation gained suspension of 4000rpm, liquid phase is the composite solution of sapindust saponin and lactic acid.
Embodiment that is disclosed or that require can make or implement in the scope being no more than existing disclosed laboratory facilities above.All products described by the preferred embodiment of the present invention and/or method, refer to expressly those do not violate concept of the present invention, scope and spirit may be used for this product and/or experimental technique and following step.To all changes and the improvement of technique means in described technique, all belong to concept, the scope and spirit of the claims in the present invention definition.
Claims (9)
1. fermentation method prepares a method for sapindust saponin extract, comprises step: by soapberry extract enzymatic saccharification, then ferments with lactobacillus inoculum.
2. a kind of fermentation method as claimed in claim 1 prepares the method for sapindust saponin extract, it is characterized in that described soapberry extract, is after being pulverized by Seed of Chinese Soapberry pericarp, the product obtained by flooding.
3. a kind of fermentation method as claimed in claim 2 prepares the method for sapindust saponin extract, it is characterized in that described pulverizing is that Seed of Chinese Soapberry fruit peel powder is broken to below 1mm, described flooding is at 50-70 DEG C of temperature, adds Seed of Chinese Soapberry pericarp quality 5-7 water doubly, lixiviate 2-5h.
4. a kind of fermentation method as claimed in claim 1 prepares the method for sapindust saponin extract, it is characterized in that described enzymatic saccharification be add in cellobiase, mannase and polygalacturonase in soapberry extract one or more carry out enzymatic saccharification.
5. a kind of fermentation method as described in claim 1 or 4 prepares the method for sapindust saponin extract, it is characterized in that described enzymatic saccharification be to pH value be add in the soapberry extract of 4.6-5.0 that consumption is the cellobiase of 8-12IU, one or more in the polygalacturonase of the mannase of 15-25IU or 3-4IU carry out enzymatic saccharification, the time of enzymatic saccharification is 80-100h, and temperature is 30-50 DEG C.
6. a kind of fermentation method as claimed in claim 1 prepares the method for sapindust saponin extract, and it is characterized in that the fermentation of described lactobacillus inoculum is under pH value 6.8-7.5 condition, the quality of inoculating lactic acid bacterium is the 0.4-0.6% of enzymatic saccharification product quality.
7. a kind of fermentation method as described in claim 1 or 6 prepares the method for sapindust saponin extract, it is characterized in that adding minimal medium or yeast extract medium when lactobacillus inoculum ferments, described minimal medium consists of: MgSO47H2O0.4-0.6g/L, KH2PO30.4-0.6g/L, NaCl0.09-0.11g/L; Described yeast extract medium consists of: MgSO47H2O0.4-0.6g/L, KH2PO30.4-0.6g/L, NaCl0.09-0.11g/L, yeast extract paste 5g/L.
8. a kind of fermentation method as described in claim 1 or 6 prepares the method for sapindust saponin extract, and it is characterized in that the temperature that described lactobacillus inoculum ferments is 40-48 DEG C, fermentation time is 140-160h.
9. a kind of fermentation method as claimed in claim 1 prepares the method for sapindust saponin extract, characterized by further comprising the step of the rear solid-liquid separation of lactobacillus inoculum fermentation, gets the product liquid after separation.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102334193B1 (en) * | 2021-02-23 | 2021-12-03 | 주식회사 네오캠코리아 | Composition for skin cleansing comprising fermented extract of soapberry and manufacturing method thereof |
-
2015
- 2015-12-27 CN CN201510987596.3A patent/CN105420297A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102334193B1 (en) * | 2021-02-23 | 2021-12-03 | 주식회사 네오캠코리아 | Composition for skin cleansing comprising fermented extract of soapberry and manufacturing method thereof |
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