CN105420204A - Laccase/lignocellulose raw material steam explosion hydrolysate natural catalytic system and application thereof - Google Patents
Laccase/lignocellulose raw material steam explosion hydrolysate natural catalytic system and application thereof Download PDFInfo
- Publication number
- CN105420204A CN105420204A CN201510965199.6A CN201510965199A CN105420204A CN 105420204 A CN105420204 A CN 105420204A CN 201510965199 A CN201510965199 A CN 201510965199A CN 105420204 A CN105420204 A CN 105420204A
- Authority
- CN
- China
- Prior art keywords
- laccase
- quick
- fried
- vapour
- wws
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
- C12N9/0057—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
- C12N9/0061—Laccase (1.10.3.2)
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y110/00—Oxidoreductases acting on diphenols and related substances as donors (1.10)
- C12Y110/03—Oxidoreductases acting on diphenols and related substances as donors (1.10) with an oxygen as acceptor (1.10.3)
- C12Y110/03002—Laccase (1.10.3.2)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Compounds Of Unknown Constitution (AREA)
- Enzymes And Modification Thereof (AREA)
- Fats And Perfumes (AREA)
Abstract
The invention discloses a laccase/lignocellulose raw material steam explosion hydrolysate natural catalytic system and application thereof, and mainly aims to directly form a laccase natural mediator catalytic system from lignocellulose raw material and laccase by fully using the characteristic that lignocellulose raw material steam explosion hydrolysate is rich in micromolecule lignin degradation product, and solves the problems that a natural mediator is high in separation and purification cost and a single mediator is low in catalytic efficiency in an existing method; meanwhile environment friendless and high-value comprehensive utilization of the steam explosion hydrolysate are realized. The technical scheme comprises (1) steam explosion pretreatment on the lignocellulose raw material and preparation of the steam explosion hydrolysate (SEL-WWS); (2) forming a laccase/mediator system from the SEL-WWS and the laccase, and applying the laccase/mediator system to biological pretreatment on a natural wood fiber raw material, biodegradation on lignin, dye decolorizing and the like. By adopting the technical scheme, the separation cost of a laccase natural mediator is expected to be lowered, and the catalytic efficiency of the mediator is improved; a new method is provided for green high-value utilization of the steam explosion hydrolysate.
Description
[technical field]
The invention belongs to biological chemical field, particularly a kind of " laccase/quick-fried hydrolyzed solution of lignocellulosic material vapour " native catalytic system and application thereof.
[background technology]
Laccase is considered to one of oxydo-reductase of the most important and most industrialization prospect of futurity industry biocatalysis field, and has caused the research boom both at home and abroad to laccase.But, because the laccase of low redox potential (0.5 ~ 0.8V) only can the phenolic lignin building unit of direct oxidation degraded tool low redox potential.Then can not be directly oxidized by laccase for the non-phenolic structural unit accounting for the overwhelming majority in xylogen total amount, but need by some micromolecular compounds as redox mediators (redoxmediator), realize the electron transmission between laccase and the substrate such as non-phenols and macromole lignin.And the Application Areas of laccase and catalyst system thereof has further been widened in the discovery of amboceptor.
But the laccase amboceptor of main application is at present synthesis amboceptor, as 2,2 '-azine-3-ethylbenzene-dithiazole-6 sulfonic acid (ABTS), I-hydroxybenzotriazole (1-hydroxybenzotriazole, HBT) etc., not only market value is expensive for they, and difficulty guarantees that itself or derivatives thereof does not have toxicity, therefore, laccase-mediator systems is so far made to be applied to industrial production on a large scale not yet.In recent years, the excavation of Laccase Catalyzed natural amboceptor has become the focus of Chinese scholars research.The natural amboceptor that some lignin degradation products react as Laccase Catalyzed, causes the dense research interest of Chinese scholars owing to having the advantage of environment and ecology.Known lacquer enzyme catalysis amboceptor, as phenolic compounds such as syringic aldehyde, Syringylethanone, Vanillin, Acetovanillone, vanillic acid methyl esters, P-coumaric acid, xylenol, vanillal, methyl vanilla acid esters, trimethoxy phenol.From existing research, the catalytic degradation effect of many natural amboceptors is suitable with synthetic amboceptor, is even better than synthesizing amboceptor.
Be rich in lignin component in lignocellulosic material, and many xylogen small molecule derivative are also proved the natural amboceptor that can be used as laccase.Lignocellulosic material is after steam explosion pre-treatment, and wherein softening and Partial digestion can occur lignin component, thus produces the materials such as phenolic acid, alcohol, aldehyde, as phenolic compound (pyrocatechol); Alcohol compound (2-hydroxylphenylethyl alcohol); Ketone compounds (4-(4-methoxy phenolic group)-2-butanone); Aldehyde compound (4-hydroxy benzaldehyde, Vanillin and syringaldehyde etc.); Acid compounds (acid of 4-methoxyphenylacetic acid, 4-HBA, 4-hydroxyl phenylacetic acid, vanillic acid, 3-methoxyl group-4 hydroxyl phenylacetic acid, Syringic acid and 4-Vinyl phenol) etc.Therefore, the quick-fried hydrolyzed solution of vapour can regard the mixture that is rich in multiple laccase small molecules amboceptor as.But, because vapour quick-fried hydrolyzed solution small molecular lignin derivative is to follow-up fermentable toxicity more or less, make it be considered to be objectionable impurities always and go out of use.Although in recent years have about extracting the report of aldehydes matter as laccase amboceptor from lignocellulosic material lignin degradation products, there is separation and purification cost high, the problems such as catalytic efficiency is lower.The present invention is based on the quick-fried hydrolyzed solution of lignocellulose vapour (WWS-SEL) is the mixing mediator systems being rich in multiple small molecules laccase amboceptor, itself and laccase is proposed to be configured to laccase/WWS-SEL system, on the one hand for vapour quick-fried lignocellulosic material hydrolyzed solution provides new utilization ways and reduces the cost of laccase amboceptor, on the other hand by the structure of laccase/many amboceptors catalyst system, improve the catalytic efficiency of laccase.
[summary of the invention]
[goal of the invention]
The object of the present invention is to provide a kind of laccase lignocellulose pre-treatment hydrolyzed solution native catalytic system and application method thereof.By hydrolyzed solution catalyst system provided by the invention and application method thereof, abandon and be existingly waste depending on hydrolyzed solution, dislike and avoid the idea of thing, make full use of the feature that hydrolyzed solution is rich in multiple laccase natural amboceptor, overcome the problem that in existing method, high, the single amboceptor catalytic efficiency of natural amboceptor separation and purification cost is low, provide reference for the green clean and effective realizing hydrolyzed solution utilizes.
[inventive concept]
Lignocellulosic material pre-treatment hydrolyzed solution is extremely complicated organic polymer blend, contains the degraded product from hemicellulose, xylogen etc., as materials such as carbohydrate, small molecules phenols, organic acid, furans.For a long time, above-mentioned substance contained in lignocellulose pre-treatment hydrolyzed solution has suppression in various degree or toxic action to microorganism and enzyme, thus to be taken as be dislike and avoid thing and go out of use always, or regarded " inhibition " and adopt various method to remove.In fact, the small molecules aldehydes matter in hydrolyzed solution can as the potential natural amboceptor of laccase, and multiple aldehydes matter coexists and likely forms laccase many amboceptors continuous catalysis system, and realizes the efficient catalytic of laccase mediator systems.Therefore the present invention regards lignocellulose pre-treatment hydrolyzed solution as complex system that multiple natural phenolic material coexists, itself and laccase are formed laccase/many amboceptors continuous catalysis system, to reaching stalk pre-treatment hydrolyzed solution green high-value-use, laccase amboceptor catalyst system low cost, high efficiency object.
[technical scheme of the present invention]
One of the present invention " laccase/quick-fried hydrolyzed solution of lignocellulosic material vapour " native catalytic system and application thereof, comprise the steps:
1) the quick-fried pre-treatment of lignocellulosic material vapour, obtains the quick-fried lignocellulosic material of vapour; The quick-fried lignocellulosic material of vapour, through washing, filter, centrifugal after, supernatant liquor is the quick-fried water lotion of vapour (SEL-WWS);
2) laccase mixes with SEL-WWS, prepares laccase/SEL-WWS natural amboceptor system, and is applied to natural wooden fiber's raw material Biological Pretreatment, lignin biodegradation, dye decolored etc.
Step 1) in, lignocellulosic material is switched to about 3-5cm, and washes 3 limits removal earth and dusts etc. with deionized water.The raw material prescinded mixes according to solid-to-liquid ratio 1:0.5 ~ 1:2 (W/W) with water, puts into steam-explosion jar, ties up pressure 2 ~ 10min and carry out Steam explosion treatment under 1.0 ~ 2.0MPa pressure, namely obtains the quick-fried lignocellulosic material of vapour (SEL).
Step 1) in, the quick-fried lignocellulosic material of vapour adds distilled water immersion and washes, repeat washing three times, filter, merge water lotion, regulate the solid-to-liquid ratio of the quick-fried raw material dry weight of final vapour and water lotion to be 1:5 ~ 1:15 (W/W), water lotion centrifugal segregation solid impurity, obtain the quick-fried pretreated water washing lotion (SEL-WWS) of vapour;
Step 2) in, SEL-WWS mixes with laccase or immobilization laccase according to total phenol content 0.1mg-10mg, prepares laccase/SEL-WWS natural amboceptor system.
Step 2) in, the condition that laccase/SEL-WWS is applied to natural wooden fiber's raw material Biological Pretreatment is: pending lignocellulosic material is first crushed to≤40 object powder, then laccase solution is added according to 5-50U/g, SEL-WWS is added according to total phenol content 0.1mg-10mg, and adding distil water regulates final solid-to-liquid ratio to be 1:10, under optimum temperuture needed for laccase, with 150rpm vibration pre-treatment 12 ~ 48h, the change measuring content of lignin in raw material characterizes laccase/SEL-WWS pretreating effect.
Step 2) in, the condition that laccase/SEL-WWS is applied to lignin biodegradation is: by 5-50U/g laccase solution, the SEL-WWS of total phenol content 0.1mg-10mg mixes with xylogen, adding distil water regulates final solid-to-liquid ratio to be 1:10, under optimum temperuture needed for laccase, with 150rpm vibration pre-treatment 12 ~ 48h, measure soluble lignin content and lignin residue residue dry weight in supernatant liquor, in order to characterize the degradation rate of xylogen.
Step 2) in, laccase/SEL-WWS is applied to dye decolored condition and is: the addition of SEL-WWS is 4-40 μ g/g dyestuff, and laccase add-on is 10-50U/g dyestuff, in 50 DEG C of decolouring 2-24h, with the dye decolored rate of the change calculations of absorbancy.
Lignocellulosic material of the present invention comprises: maize straw, straw, rice straw, timber, forest land weeds.
Xylogen of the present invention comprises: enzymolysis xylogen, alkali lignin, Milled wood lignin.
Dyestuff of the present invention comprises: Viola crystallina, Victoria Green WPB, Methylene blue, phenol red.
[advantage of the present invention]
The present invention break traditions depending on the quick-fried pretreated water washing lotion of lignocellulosic material vapour be dislike and avoid thing to carry out detoxification treatment after carry out the thinking of biological utilisation again, but regard it as the many mediator systems being rich in multiple natural phenolic laccase amboceptor, by building laccase/many amboceptors continuous catalysis system, to reaching stalk pre-treatment hydrolyzed solution green high-value-use, laccase amboceptor catalyst system low cost, high efficiency object.Meanwhile, the green resourceization that can realize lignocellulose pre-treatment hydrolyzed solution utilizes.
[specific implementation method]
Embodiment 1
(1) the quick-fried pre-treatment of raw material vapour: maize straw is switched to about 3-5cm, and wash 3 times removals earth and dusts etc. with deionized water.The maize straw prescinded mixes according to solid-to-liquid ratio 1:0.5 (W/W) with water, puts into steam-explosion jar, and under 1.0MPa pressure, dimension pressure 5min carries out Steam explosion treatment, namely obtains the quick-fried lignocellulosic material of vapour (SECS).
(2) preparation of vapour quick-fried pretreated water washing lotion: step 1) in the quick-fried lignocellulosic material of vapour add distilled water immersion and wash, repeat washing three times, filter, merge water lotion, the solid-to-liquid ratio of the quick-fried raw material dry weight of final vapour and water lotion is regulated to be 1:10 (W/W), water lotion centrifugal segregation solid impurity, obtains the quick-fried pretreated water washing lotion (SECS-WWS) of vapour;
(3) laccase/SECS-WWS native catalytic system is applied to natural wooden fiber's raw material Biological Pretreatment: pending lignocellulosic material is first crushed to≤40 object powder, then laccase solution is added according to 5U/g, SECS-WWS is added according to total phenol content 0.1mg, and adding distil water regulates final solid-to-liquid ratio to be 1:10, at 50 DEG C, with 150rpm vibration pre-treatment 12h, the change measuring content of lignin in raw material characterizes laccase/SECS-WWS pretreating effect.After pre-treatment, in raw material, the content of xylogen reduces by 15.2%, and relative to laccase/ABTS control group, in raw material, Lignin degradation rate improves 35.6%.
Embodiment 2
(1) the quick-fried pre-treatment of raw material vapour: straw is switched to about 3-5cm, and wash 3 times removals earth and dusts etc. with deionized water.The straw prescinded mixes according to solid-to-liquid ratio 1:0.5 (W/W) with water, puts into steam-explosion jar, and under 1.5MPa pressure, dimension pressure 5min carries out Steam explosion treatment, namely obtains the quick-fried straw of vapour (SEWS).
(2) preparation of vapour quick-fried pretreated water washing lotion: step 1) in the quick-fried straw of vapour add distilled water immersion and wash, repeat washing three times, filter, merge water lotion, the solid-to-liquid ratio of the quick-fried raw material dry weight of final vapour and water lotion is regulated to be 1:10 (W/W), water lotion centrifugal segregation solid impurity, obtains the quick-fried pretreated water washing lotion (SEWS-WWS) of vapour;
(3) laccase/SEWS-WWS native catalytic system is applied to the Biological Pretreatment of wood chip: pending wood chip is first crushed to≤40 object powder, then laccase solution is added according to 20U/g, SEWS-WWS is added according to total phenol content 2mg, and adding distil water regulates final solid-to-liquid ratio to be 1:10, at 50 DEG C, with 150rpm vibration pre-treatment 24h, the change measuring content of lignin in wood chip characterizes laccase/SEWS-WWS pretreating effect.After pre-treatment, in raw material, the content of xylogen reduces by 23.4%, and relative to laccase/ABTS control group, in raw material, Lignin degradation rate improves 20.4%.
Embodiment 3
(1) the quick-fried pre-treatment of raw material vapour: rice straw is switched to about 3-5cm, and wash 3 times removals earth and dusts etc. with deionized water.The rice straw prescinded mixes according to solid-to-liquid ratio 1:0.5 (W/W) with water, puts into steam-explosion jar, and under 1.6MPa pressure, dimension pressure 8min carries out Steam explosion treatment, namely obtains the quick-fried rice straw of vapour (SERS).
(2) preparation of vapour quick-fried pretreated water washing lotion: step 1) in the quick-fried rice straw of vapour add distilled water immersion and wash, repeat washing three times, filter, merge water lotion, the solid-to-liquid ratio of the quick-fried raw material dry weight of final vapour and water lotion is regulated to be 1:10 (W/W), water lotion centrifugal segregation solid impurity, obtains the quick-fried rice straw water lotion (SERS-WWS) of vapour;
(3) laccase/SECS-WWS native catalytic system is applied to the Biological Pretreatment of forest land weeds: pending weeds is first crushed to≤40 object powder, then laccase solution is added according to 25U/g, SECS-WWS is added according to total phenol content 5mg, and adding distil water regulates final solid-to-liquid ratio to be 1:10, at 50 DEG C, with 150rpm vibration pre-treatment 36h, the change measuring content of lignin in raw material characterizes laccase/SECS-WWS pretreating effect.After pre-treatment, in raw material, the content of xylogen reduces by 22.8%, and relative to laccase/ABTS control group, in raw material, Lignin degradation rate improves 21.5%.
Embodiment 4
(1) the quick-fried pre-treatment of raw material vapour: wood chip is switched to about 3-5cm, and wash 3 times removals earth and dusts etc. with deionized water.The wood chip prescinded mixes according to solid-to-liquid ratio 1:0.5 (W/W) with water, puts into steam-explosion jar, and under 1.8MPa pressure, dimension pressure 10min carries out Steam explosion treatment, namely obtains the quick-fried wood chip of vapour (SEW).
(2) preparation of vapour quick-fried pretreated water washing lotion: step 1) in the quick-fried wood chip of vapour add distilled water immersion and wash, repeat washing three times, filter, merge water lotion, the solid-to-liquid ratio of the quick-fried raw material dry weight of final vapour and water lotion is regulated to be 1:10 (W/W), water lotion centrifugal segregation solid impurity, obtains the quick-fried wood chip water lotion (SEW-WWS) of vapour;
(3) laccase/SEW-WWS native catalytic system is applied to the Biological Pretreatment of wood chip: pending wood dust is broken to≤40 object powder, then laccase solution is added according to 50U/g, SECS-WWS is added according to total phenol content 10mg, and adding distil water regulates final solid-to-liquid ratio to be 1:10, at 50 DEG C, with 150rpm vibration pre-treatment 48h, the change measuring content of lignin in raw material characterizes laccase/SECS-WWS pretreating effect.After pre-treatment, in raw material, the content of xylogen reduces by 26.4%, and relative to laccase/ABTS control group, in raw material, Lignin degradation rate improves 33.2%.
Embodiment 5
(1) the quick-fried pre-treatment of raw material vapour: wood chip is switched to about 3-5cm, and wash 3 times removals earth and dusts etc. with deionized water.The wood chip prescinded mixes according to solid-to-liquid ratio 1:0.5 (W/W) with water, puts into steam-explosion jar, and under 1.8MPa pressure, dimension pressure 10min carries out Steam explosion treatment, namely obtains the quick-fried wood chip of vapour (SEW).
(2) preparation of vapour quick-fried pretreated water washing lotion: step 1) in the quick-fried wood chip of vapour add distilled water immersion and wash, repeat washing three times, filter, merge water lotion, the solid-to-liquid ratio of the quick-fried raw material dry weight of final vapour and water lotion is regulated to be 1:10 (W/W), water lotion centrifugal segregation solid impurity, obtains the quick-fried wood chip water lotion (SEW-WWS) of vapour;
(3) laccase/SEW-WWS native catalytic system is applied to the biological degradation of xylogen: mix with SEW-WWS and the 2g Heating Explosion Sugarcane Bagasse xylogen of 5U/g laccase solution, total phenol content 0.1mg, adding distil water regulates final solid-to-liquid ratio to be 1:10, at 50 DEG C, with 150rpm vibration pre-treatment 12h, the degradation rate measuring xylogen is 11.4%, relative to laccase/ABTS control group, Lignin degradation rate improves 16.4%.
Embodiment 6
(1) the quick-fried pre-treatment of raw material vapour: maize straw is switched to about 3-5cm, and wash 3 times removals earth and dusts etc. with deionized water.The wood chip prescinded mixes according to solid-to-liquid ratio 1:0.5 (W/W) with water, puts into steam-explosion jar, and under 1.5MPa pressure, dimension pressure 5min carries out Steam explosion treatment, namely obtains the quick-fried maize straw of vapour (SECS).
(2) preparation of vapour quick-fried pretreated water washing lotion: step 1) in the quick-fried maize straw of vapour add distilled water immersion and wash, repeat washing three times, filter, merge water lotion, the solid-to-liquid ratio of the quick-fried raw material dry weight of final vapour and water lotion is regulated to be 1:10 (W/W), water lotion centrifugal segregation solid impurity, obtains the quick-fried maize straw water lotion (SECS-WWS) of vapour;
(3) laccase/SECS-WWS native catalytic system is applied to the biological degradation of xylogen: mix with SECS-WWS and the 2g alkali lignin of 20U/g laccase solution, total phenol content 1mg, adding distil water regulates final solid-to-liquid ratio to be 1:10, at 50 DEG C, with 150rpm vibration pre-treatment 24h, the degradation rate measuring xylogen is 22.6%, relative to laccase/ABTS control group, Lignin degradation rate improves 26.5%.
Embodiment 7
(1) the quick-fried pre-treatment of raw material vapour: maize straw is switched to about 3-5cm, and wash 3 times removals earth and dusts etc. with deionized water.The wood chip prescinded mixes according to solid-to-liquid ratio 1:0.5 (W/W) with water, puts into steam-explosion jar, and under 1.6MPa pressure, dimension pressure 10min carries out Steam explosion treatment, namely obtains the quick-fried maize straw of vapour (SECS).
(2) preparation of vapour quick-fried pretreated water washing lotion: step 1) in the quick-fried maize straw of vapour add distilled water immersion and wash, repeat washing three times, filter, merge water lotion, the solid-to-liquid ratio of the quick-fried raw material dry weight of final vapour and water lotion is regulated to be 1:10 (W/W), water lotion centrifugal segregation solid impurity, obtains the quick-fried maize straw water lotion (SECS-WWS) of vapour;
(3) laccase/SECS-WWS native catalytic system is applied to the biological degradation of xylogen: mix with SECS-WWS and the 2g Milled wood lignin of 50U/g laccase solution, total phenol content 10mg, adding distil water regulates final solid-to-liquid ratio to be 1:10, at 50 DEG C, with 150rpm vibration pre-treatment 48h, the degradation rate measuring xylogen is 46.5%, relative to laccase/ABTS control group, Lignin degradation rate improves 43.8%.
Embodiment 8
(1) the quick-fried pre-treatment of raw material vapour: maize straw is switched to about 3-5cm, and wash 3 times removals earth and dusts etc. with deionized water.The wood chip prescinded mixes according to solid-to-liquid ratio 1:0.5 (W/W) with water, puts into steam-explosion jar, and under 1.6MPa pressure, dimension pressure 10min carries out Steam explosion treatment, namely obtains the quick-fried maize straw of vapour (SECS).
(2) preparation of vapour quick-fried pretreated water washing lotion: step 1) in the quick-fried maize straw of vapour add distilled water immersion and wash, repeat washing three times, filter, merge water lotion, the solid-to-liquid ratio of the quick-fried raw material dry weight of final vapour and water lotion is regulated to be 1:10 (W/W), water lotion centrifugal segregation solid impurity, obtains the quick-fried maize straw water lotion (SECS-WWS) of vapour;
(3) laccase/SECS-WWS native catalytic system is applied to dye decolored: the addition of SECS-WWS is 4 μ g/g Viola crystallinas, laccase add-on is 10U/g Viola crystallina, in 50 DEG C of decolouring 2h, the percent of decolourization of Viola crystallina is 28.5%, relative to laccase/ABTS control group, the percent of decolourization of Viola crystallina improves 1.88 times.
Embodiment 9
(1) the quick-fried pre-treatment of raw material vapour: wood chip is switched to about 3-5cm, and wash 3 times removals earth and dusts etc. with deionized water.The wood chip prescinded mixes according to solid-to-liquid ratio 1:0.5 (W/W) with water, puts into steam-explosion jar, and under 1.8MPa pressure, dimension pressure 6min carries out Steam explosion treatment, namely obtains the quick-fried wood chip of vapour (SEW).
(2) preparation of vapour quick-fried pretreated water washing lotion: step 1) in the quick-fried wood chip of vapour add distilled water immersion and wash, repeat washing three times, filter, merge water lotion, the solid-to-liquid ratio of the quick-fried raw material dry weight of final vapour and water lotion is regulated to be 1:10 (W/W), water lotion centrifugal segregation solid impurity, obtains the quick-fried wood chip water lotion (SEW-WWS) of vapour;
(3) laccase/SECS-WWS native catalytic system is applied to dye decolored: the addition of SECS-WWS is 15 μ g/g Victoria Green WPBs, laccase add-on is 20U/g Victoria Green WPB, in 50 DEG C of decolouring 8h, the percent of decolourization of Victoria Green WPB is 73.6%, relative to laccase/ABTS control group, the percent of decolourization of Victoria Green WPB improves 2.18 times.
Embodiment 10
(1) the quick-fried pre-treatment of raw material vapour: straw is switched to about 3-5cm, and wash 3 times removals earth and dusts etc. with deionized water.The straw prescinded mixes according to solid-to-liquid ratio 1:0.5 (W/W) with water, puts into steam-explosion jar, and under 1.6MPa pressure, dimension pressure 5min carries out Steam explosion treatment, namely obtains the quick-fried straw of vapour (SEWS).
(2) preparation of vapour quick-fried pretreated water washing lotion: step 1) in the quick-fried straw of vapour add distilled water immersion and wash, repeat washing three times, filter, merge water lotion, the solid-to-liquid ratio of the quick-fried raw material dry weight of final vapour and water lotion is regulated to be 1:10 (W/W), water lotion centrifugal segregation solid impurity, obtains the quick-fried pretreated water washing lotion (SEWS-WWS) of vapour;
(3) laccase/SECS-WWS native catalytic system is applied to dye decolored: the addition of SECS-WWS is 25 μ g/g Methylene blues, and laccase add-on is 50U/g Methylene blue, and in 50 DEG C of decolouring 12h, the percent of decolourization of Methylene blue is 33.1%.In control group, the different catalysis Methylene blue decolouring of laccase/ABTS catalyst system.
Embodiment 11
(1) the quick-fried pre-treatment of raw material vapour: maize straw is switched to about 3-5cm, and wash 3 times removals earth and dusts etc. with deionized water.The wood chip prescinded mixes according to solid-to-liquid ratio 1:0.5 (W/W) with water, puts into steam-explosion jar, and under 1.4MPa pressure, dimension pressure 6min carries out Steam explosion treatment, namely obtains the quick-fried maize straw of vapour (SECS).
(2) preparation of vapour quick-fried pretreated water washing lotion: step 1) in the quick-fried maize straw of vapour add distilled water immersion and wash, repeat washing three times, filter, merge water lotion, the solid-to-liquid ratio of the quick-fried raw material dry weight of final vapour and water lotion is regulated to be 1:10 (W/W), water lotion centrifugal segregation solid impurity, obtains the quick-fried maize straw water lotion (SECS-WWS) of vapour;
(3) laccase/SECS-WWS native catalytic system is applied to dye decolored: the addition of SECS-WWS is that 40 μ g/g are phenol red, laccase add-on is that 50U/g is phenol red, in 50 DEG C of decolouring 24h, phenol red percent of decolourization is 58.4%, relative to laccase/ABTS control group, phenol red percent of decolourization improves 5.26 times.
Claims (10)
1. one of the present invention " laccase/quick-fried hydrolyzed solution of lignocellulosic material vapour " native catalytic system and application thereof, comprise the steps:
1) the quick-fried pre-treatment of lignocellulosic material vapour, obtains the quick-fried lignocellulosic material of vapour; The quick-fried lignocellulosic material of vapour, through washing, filter, centrifugal after, supernatant liquor is the quick-fried water lotion of vapour (SEL-WWS);
2) laccase mixes with SEL-WWS, prepares laccase/SEL-WWS natural amboceptor system, and is applied to natural wooden fiber's raw material Biological Pretreatment, lignin biodegradation, dye decolored etc.
2. one according to claim 1 " laccase/quick-fried hydrolyzed solution of lignocellulosic material vapour " native catalytic system, is characterized in that step 1) in, lignocellulosic material is switched to about 3-5cm, and washes 3 times removals earth and dusts etc. with deionized water.The raw material prescinded mixes according to solid-to-liquid ratio 1:0.5 ~ 1:2 (W/W) with water, puts into steam-explosion jar, ties up pressure 2 ~ 10min and carry out Steam explosion treatment under 1.0 ~ 2.0MPa pressure, namely obtains the quick-fried lignocellulosic material of vapour (SEL).
3. one according to claim 1 " laccase/quick-fried hydrolyzed solution of lignocellulosic material vapour " native catalytic system, it is characterized in that step 1) in, the quick-fried lignocellulosic material of vapour adds distilled water immersion and washes, repeat washing three times, filter, merge water lotion, regulate the solid-to-liquid ratio of the quick-fried raw material dry weight of final vapour and water lotion to be 1:5 ~ 1:15 (W/W), water lotion centrifugal segregation solid impurity, obtains the quick-fried pretreated water washing lotion (SEL-WWS) of vapour.
4. one according to claim 1 " laccase/quick-fried hydrolyzed solution of lignocellulosic material vapour " native catalytic system, it is characterized in that step 2) in, SEL-WWS mixes with laccase according to total phenol content 0.1mg-10mg, prepares laccase/SEL-WWS natural amboceptor system.
5. one according to claim 1 " laccase/quick-fried hydrolyzed solution of lignocellulosic material vapour " native catalytic system, it is characterized in that step 2) in, the condition that laccase/SEL-WWS is applied to natural wooden fiber's raw material Biological Pretreatment is: pending lignocellulosic material is first crushed to≤40 object powder, then laccase solution is added according to 5-50U/g, SEL-WWS is added according to total phenol content 0.1mg-10mg, and adding distil water regulates final solid-to-liquid ratio to be 1:10, under optimum temperuture needed for laccase, with 150rpm vibration pre-treatment 12 ~ 48h, the change measuring content of lignin in raw material characterizes laccase/SEL-WWS pretreating effect.
6. one according to claim 1 " laccase/quick-fried hydrolyzed solution of lignocellulosic material vapour " native catalytic system, it is characterized in that step 2) in, the condition that laccase/SEL-WWS is applied to lignin biodegradation is: by 5-50U/g laccase solution, the SEL-WWS of total phenol content 0.1mg-10mg mixes with xylogen, adding distil water regulates final solid-to-liquid ratio to be 1:10, under optimum temperuture needed for laccase, with 150rpm vibration pre-treatment 12 ~ 48h, measure soluble lignin content and lignin residue residue dry weight in supernatant liquor, in order to characterize the degradation rate of xylogen.
7. one according to claim 1 " laccase/quick-fried hydrolyzed solution of lignocellulosic material vapour " native catalytic system, it is characterized in that step 2) in, laccase/SEL-WWS is applied to dye decolored condition: the addition of SEL-WWS is 4-40 μ g/g dyestuff, laccase add-on is 10-50U/g dyestuff, in 50 DEG C of decolouring 2-24h, with the dye decolored rate of the change calculations of absorbancy.
8. lignocellulosic material according to claim 5 comprises: maize straw, straw, rice straw, timber, forest land weeds.
9. xylogen according to claim 6 comprises: enzymolysis xylogen, alkali lignin, Milled wood lignin.
10. dyestuff according to claim 7 comprises: Viola crystallina, Victoria Green WPB, Methylene blue, phenol red.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510965199.6A CN105420204A (en) | 2015-12-21 | 2015-12-21 | Laccase/lignocellulose raw material steam explosion hydrolysate natural catalytic system and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510965199.6A CN105420204A (en) | 2015-12-21 | 2015-12-21 | Laccase/lignocellulose raw material steam explosion hydrolysate natural catalytic system and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105420204A true CN105420204A (en) | 2016-03-23 |
Family
ID=55498746
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510965199.6A Pending CN105420204A (en) | 2015-12-21 | 2015-12-21 | Laccase/lignocellulose raw material steam explosion hydrolysate natural catalytic system and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105420204A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112761012A (en) * | 2020-12-25 | 2021-05-07 | 天津科技大学 | Method for pretreating poplar by temperature-pressure separate control gas explosion technology |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105039171A (en) * | 2015-07-03 | 2015-11-11 | 安徽工程大学 | Trametes sp. and application thereof |
-
2015
- 2015-12-21 CN CN201510965199.6A patent/CN105420204A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105039171A (en) * | 2015-07-03 | 2015-11-11 | 安徽工程大学 | Trametes sp. and application thereof |
Non-Patent Citations (4)
| Title |
|---|
| R.MARTIN-SAMPEDRO ET AL.: "Steam explosion and enzymatic pre-treatments as an approach to improve the enzymatic hydrolysis of Eucalyptus globulus.", 《BIOMASS AND BIOENERGY》 * |
| WEIHUA QIU ET AL.: "Natural laccase mediators separated from water-washed solution of steam exploded corn straw by nanofiltration and organic solvent fractionation", 《BIORESOURCE TECHNOLOGY》 * |
| 付时雨等: "漆酶/介体催化体系中介体的反应性能", 《中国造纸》 * |
| 邱卫华等: "木质纤维素原料中漆酶天然介体的研究进展", 《生物工程学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112761012A (en) * | 2020-12-25 | 2021-05-07 | 天津科技大学 | Method for pretreating poplar by temperature-pressure separate control gas explosion technology |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Xing et al. | Novel dihydrogen-bonding deep eutectic solvents: Pretreatment of rice straw for butanol fermentation featuring enzyme recycling and high solvent yield | |
| AU2018201505B2 (en) | Method of converting lignin and uses thereof | |
| Zhai et al. | What are the major components in steam pretreated lignocellulosic biomass that inhibit the efficacy of cellulase enzyme mixtures? | |
| Teixeira et al. | Optimizing peracetic acid pretreatment conditions for improved simultaneous saccharification and co-fermentation (SSCF) of sugar cane bagasse to ethanol fuel | |
| Dharmaraja et al. | Lignocellulosic biomass conversion via greener pretreatment methods towards biorefinery applications | |
| Yin et al. | The pretreatment of lignocelluloses with green solvent as biorefinery preprocess: a minor review | |
| Trinh et al. | Aqueous acidified ionic liquid pretreatment for bioethanol production and concentration of produced ethanol by pervaporation | |
| US20100263814A1 (en) | Pretreatment of lignocellulosic biomass through removal of inhibitory compounds | |
| Yu et al. | Improvement of the enzymatic hydrolysis of furfural residues by pretreatment with combined green liquor and ethanol organosolv | |
| Gong et al. | Hydrogen peroxide-assisted sodium carbonate pretreatment for the enhancement of enzymatic saccharification of corn stover | |
| CN101765663A (en) | biomass pretreatment | |
| Chen et al. | Furfural production from steam explosion liquor of rice straw by solid acid catalysts (HZSM-5) | |
| CN105330869B (en) | A kind of method for hydrolysis of lignocellulose raw material | |
| Trinh et al. | Optimization of ionic liquid pretreatment of mixed softwood by response surface methodology and reutilization of ionic liquid from hydrolysate | |
| Jafari et al. | Construction and demolition lignocellulosic wastes to bioethanol | |
| JP2011092151A (en) | Method of processing vegetable raw material | |
| Coca et al. | Chemical oxidation with ozone as an efficient pretreatment of lignocellulosic materials | |
| KR20150023187A (en) | A method for manufacturing pellets for fuel using dis-used badges or logs after cultivation of mushrooms | |
| Rajamani et al. | Value-added chemicals from sugarcane bagasse using ionic liquids | |
| Chaudhary et al. | Assessment of pretreatment strategies for valorization of lignocellulosic biomass: path forwarding towards lignocellulosic biorefinery | |
| Chen et al. | Combinatorial pretreatments of reed straw using liquid hot water and lactic acid for fermentable sugar production | |
| Pant et al. | Pretreatment of lignocellulosic biomass for bioethanol production | |
| Bhagwat et al. | Biomass pre-treatment methods and their economic viability for efficient production of biofuel | |
| CN105420204A (en) | Laccase/lignocellulose raw material steam explosion hydrolysate natural catalytic system and application thereof | |
| Bajpai | Deep eutectic solvents for pretreatment of lignocellulosic biomass |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160323 |
|
| WD01 | Invention patent application deemed withdrawn after publication |