CN105413766A - Micro-fluidic chip and separation channel for electrophoretic separation - Google Patents
Micro-fluidic chip and separation channel for electrophoretic separation Download PDFInfo
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- CN105413766A CN105413766A CN201510727483.XA CN201510727483A CN105413766A CN 105413766 A CN105413766 A CN 105413766A CN 201510727483 A CN201510727483 A CN 201510727483A CN 105413766 A CN105413766 A CN 105413766A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
- B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D57/00—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
- B01D57/02—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
- B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0636—Focussing flows, e.g. to laminate flows
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
- B01L2400/0421—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electrophoretic flow
Abstract
The present invention provides a micro-fluidic chip and a separation channel for electrophoretic separation. A center of the micro-fluidic chip is provided with a central through hole; by using the central through hole as a circular center, the micro-fluidic chip is provided with at least two radial shunt channels in a manner of radiating an edge; the radial shunt channels are the same; and the radial shunt channels are all connected with the central through hole. The separation channel for electrophoretic separation constructed by the micro-fluidic chip comprises two symmetrically arranged micro-fluidic chips which are an initial end micro-fluidic chip and a terminal micro-fluidic chip, wherein the initial end micro-fluidic chip and the terminal micro-fluidic chip opposing radial shunt channels are in one-to-one communication by a connection capillary. A porous plug is arranged in each of the radial shunt channels of the initial end micro-fluidic chip. By adopting the separation channel provided by the present invention, a total injection volume and a total flow speed of electrophoresis are increased, and high separation efficiency of capillary electrophoresis is ensured. The separation channel has the advantages of high separation efficiency, high detection sensitivity, simple structure, easy operation and low cost.
Description
(1) technical field
The present invention relates to a kind of micro-fluidic chip and electrophoretic separation split tunnel.
(2) background technology
Element morphology information in environmental and biological samples contributes to toxicity, animal migration and the bioavailability that people understand it.Atomic spectroscopic analysis technology, particularly plasma mass technology are the powerful tools of current trace element macroanalysis, but are difficult to analyze the existing forms of trace element in the complicated substrates such as environment, biology and food and content thereof.Chromatography schema category is various, applied widely, is the efficient means, particularly capillary electrophoresis technique of the different shape species of trace element in Analysis of Complex matrix, has that separative efficiency is high, the fast and sample of speed consumes the advantages such as little.Advantage both Capillary Electrophoresis is combined with plasma mass GC-MS, namely the high separating efficiency of Capillary Electrophoresis and the high sensitivity of plasma mass and high element selective, be a kind of Valence Analysis with very large potentiality.Microfluidic analysis chip has that analysis efficiency is high, sample consumption less, be easy to the features such as microminiaturized and portability, be current chemistry and biological study hotspot.Connecting line in Capillary Electrophoresis and plasma mass connecting interface and joint can be integrated on chip easily, have saved the time and the cost that make these pipelines and joint, and have reduced the dead volume of their connecting portion, also simplified combined apparatus.
But, Capillary Electrophoresis and plasma mass coupling first must design an effective interface, the flow of necessary both the compatibilities of this interface, ensures that electrophoretic separation and plasma mass measure and does not interfere with each other, electrophoresis effluent high efficiency of transmission also will be made simultaneously to plasma mass.Design such interface, need the problem solved to be how to reduce the self-priming effect of the pneumatic nebulizer generation that plasma mass spectrograph uses.The self-priming effect of atomizer can produce laminar flow in separation capillary, and the electrophoretic separation of interference different plant species even causes being separated unsuccessfully.In order to farthest reduce self-priming effect, a kind of simple effective method introduces replenisher stream.But, self-priming flow due to pneumatic nebulizer is subject to atomization gas flow, sample solution viscosity and liquid by the impact of the factors such as the distance of vertical-lift, be difficult to the self-priming flow being mated atomizer by replenisher stream completely, fine difference between the two will be unfavorable to the electrophoresis process in separation capillary.Another kind method uses cross-current atomizer to reduce self-priming effect, and in the case, atomization gas Way out is vertical with sample solution pipeline, and the self-priming flow of atomizer reduces greatly, and thus self-priming effect also greatly alleviates.But the nebulization efficiency of cross-current atomizer is not high, only has 10%.Recently, document is Yang, G., Xu, X., Wang, W., etal., Anewinterfaceusedtocouplecapillaryelectrophoresiswithind uctivelycoupledplasmamassspectrometryforspeciationanalys is [J], Electrophoresis, 2008,29 (13): the 2862-2868 new interfaces disclosing a Capillary Electrophoresis and plasma mass coupling, it completely eliminates the laminar flow phenomenon in separation capillary that atomizer self-priming causes.Electrophoresis effluent in separation capillary by collected offline, then transfers to three-way connection by peristaltic pump, is then spread by the replenisher that another peristaltic pump is carried and is passed to atomizer and is finally detected by plasma mass.After first electrophoresis effluent transfers to three-way connection, first peristaltic pump is out of service, until second electrophoresis effluent is collected complete.Because separation capillary and atomizer are kept apart by first peristaltic pump, when it is out of service, completely eliminate the self-priming effect of atomizer to the impact of electrophoretic separation.But this connecting interface is only applicable to the species that transit time difference is greater than 20s, otherwise two kinds of electrophoresis summits analyzing thing overlap.
Except self-priming effect, another problem that Capillary Electrophoresis and plasma mass coupling must be considered is the sensitivity of interface.The sample introduction flow of the Conventional nebulizer that plasma mass is used is generally 0.5-2mL/min, the sample introduction flow of microatomization device is adopted to be generally 5-100 μ L/min, this is all considerably beyond the flow velocity (sub-μ L/min level) of Capillary Electrophoresis, and therefore the interface of the overwhelming majority uses the sheath flow liquid of large discharge to balance both difference in flow.Then after separation capillary, introduce the concentration that sheath flow liquid meeting Macrodilution analyzes thing, the sensitivity of connecting interface is significantly declined.On the other hand, the sample size of Capillary Electrophoresis is generally to count to receive and rises to tens of liter of receiving, and plasma mass is a mass flow rate sensitive detector (namely sensitivity is relevant to sample size), and this also causes the sensitivity of method for combined use to make the matter worse.Because metal form species content in the matrixes such as biology, environment is lower, they are very difficult to use conventional capillary electrophoresis and plasma mass combined system direct-detection.For reducing the detection limit of combined system, can adopt and improve the method such as connecting interface, off-line or on-line sample concentration, increase sample size.The means improving connecting interface have hydride generation sample introduction, but it is of limited application (being only limitted to the element that As, Sn, Hg etc. can form hydride).Off-line or on-line sample concentration method effect are better, but device relative complex, consuming time longer.Increase sample size and can improve sensitivity pro rata, but meeting sacrificial separation degree; And the sample belt of capillary electrophoresis separation generally can not exceed 1/10 of split tunnel, otherwise will cause being separated unsuccessfully, which has limited the effect increasing sample size method.
In addition, another problem that Capillary Electrophoresis and plasma mass coupling must be considered is the dead volume of interface.The dead volume of interface is larger, and the time that analysis thing stops at this is longer, and widening of electrophoresis peak is more serious, reduces separative efficiency and detection sensitivity.Existing Capillary Electrophoresis and plasma mass connecting interface generally adopt two logical, threeway or four-way to be connected high-field electrode, separation capillary, sheath flow liquid pipeline and atomizer, their dead volume tens of liter of receiving at least, count microlitre at most, easily cause widening of electrophoresis peak.
(3) summary of the invention
For solving the problem, the object of this invention is to provide a kind of micro-fluidic chip and electrophoretic separation split tunnel that can be used for Capillary Electrophoresis and plasma mass combined system.
For achieving the above object, the present invention adopts following technical scheme:
A kind of micro-fluidic chip, the center of described micro-fluidic chip has central through hole, and described micro-fluidic chip take central through hole as the center of circle, goes out at least 2 radial split channels to fringe radiation, described radial split channel is all identical, and described radial split channel is all communicated with central through hole.
Further, described radial split channel is uniformly distributed along described micro-fluidic chip.
The electrophoretic separation split tunnel that a kind of described micro-fluidic chip builds, described split tunnel comprises symmetrically arranged two pieces of micro-fluidic chips, be respectively top micro-fluidic chip and terminal micro-fluidic chip, the radial split channel that described top fluidic chip is relative with terminal fluidic chip is all communicated with one by one by being connected capillary.And be equipped with porous plug in the radial split channel of described top micro-fluidic chip.
Further, the central through hole of top micro-fluidic chip is connected with high-field electrode, and the central through hole of terminal micro-fluidic chip is connected with earth electrode, and described high-field electrode and earth electrode are connected with the two ends of high voltage source respectively.
Further again, the central through hole place of described top micro-fluidic chip is also connected with feed liquor capillary and waste liquid capillary.
Further, the central through hole place of described top micro-fluidic chip is also connected with sheath flow liquid capillary and transfer capillary.
The invention has the beneficial effects as follows: the radial split channel of n bar is set, total sample size and electrophoresis overall flow rate are all increased to n doubly, ensure that the high separating efficiency of Capillary Electrophoresis has the advantages that separative efficiency is high, detection sensitivity is high, structure is simple, easy to operate, with low cost.
(4) accompanying drawing explanation
Fig. 1 is top micro-fluidic chip structural representation of the present invention;
Fig. 2 is split tunnel structural representation;
Fig. 3 is structural representation of the present invention;
Fig. 4 is the electrophoretogram detecting the mixed sample gained be made up of mercury ion, methyl mercury ion, phenyl mercury ion with the present invention.
In figure: 1-top micro-fluidic chip, 2-terminal micro-fluidic chip, 3-waste liquid capillary, 4-sheath flow liquid capillary, 5-feed liquor capillary, 6-shifts capillary, 7-high-field electrode, 8-earth electrode, 9-connects capillary, 10-high voltage source, 11-high pressure pump, 12-six-way injection valve, 13-waste liquid bottle, 14-syringe pump, 15-atomizer, 16-plasma mass spectrograph, the radial split channel of 17-, 18-central through hole, 19-porous plug, 20-heated nebulization chamber.
(5) detailed description of the invention
Content described in the embodiment of this description is only enumerating the way of realization of inventive concept; protection scope of the present invention should not be regarded as being only limitted to the concrete form that embodiment is stated, protection scope of the present invention also and conceive the equivalent technologies means that can expect according to the present invention in those skilled in the art.
With reference to Fig. 1-Fig. 4:
A kind of micro-fluidic chip, the center of described micro-fluidic chip has central through hole 18, described micro-fluidic chip with central through hole 18 for the center of circle, at least 2 radial split channels 17 are gone out to fringe radiation, described radial split channel 17 is all identical, and described radial split channel 17 is all communicated with central through hole 18.
Further, described radial split channel 17 is uniformly distributed along described micro-fluidic chip.
The electrophoretic separation split tunnel that a kind of described micro-fluidic chip builds, described split tunnel comprises symmetrically arranged two pieces of micro-fluidic chips, be respectively top micro-fluidic chip 1 and terminal micro-fluidic chip 2, the all logical 17 mistake connection capillaries 9 of the radial split channel that described top fluidic chip 1 is relative with terminal fluidic chip 2 are communicated with one by one, and the micro-fluidic core in described top, be equipped with porous plug 19 in the radial split channel 17 of 1.
Further, the micro-fluidic core in top, the central through hole 18 of 1 is connected with high-field electrode 7, and the central through hole 18 of terminal micro-fluidic chip 2 is connected with earth electrode 8, and described high-field electrode 7 is connected with the two ends of high voltage source 10 respectively with earth electrode 8.
Further again, central through hole 18 place of described top micro-fluidic chip 1 is also connected with feed liquor capillary 5 and waste liquid capillary 3.
Further, central through hole 18 place of described top micro-fluidic chip 2 is also connected with sheath flow liquid capillary and transfer capillary 6.
A kind of chip analysis system that chip electrophoresis is separated and plasma mass detects utilizing described electrophoretic separation split tunnel component, comprise the electrophoretic separation portion and test section that match, described electrophoretic separation portion comprises split tunnel, sample intake passage, buffer solution waste fluid channel, replenisher passage and stock layout passage, and described test section comprises plasma mass spectrograph and atomizer;
Described split tunnel comprises micro-fluidic chip, the center of described micro-fluidic chip has central through hole 18, described micro-fluidic chip with central through hole 18 for the center of circle, at least 2 radial split channels 17 are gone out to fringe radiation, described radial split channel 17 is all identical, and described radial split channel 17 is all communicated with central through hole 18;
Described split tunnel comprises symmetrically arranged two pieces of micro-fluidic chips, be respectively top micro-fluidic chip 1 and terminal micro-fluidic chip 2, the radial split channel 17 that described top fluidic chip 1 is relative with terminal fluidic chip 2 is all communicated with one by one by being connected capillary 9, and is equipped with porous plug 19 in the radial split channel 17 of described top micro-fluidic chip 1;
The central through hole 18 of top micro-fluidic chip 1 is connected with high-field electrode 7, and the central through hole 18 of terminal micro-fluidic chip 2 is connected with earth electrode 8, and described high-field electrode 7 is connected with the two ends of high voltage source 10 respectively with earth electrode 8;
Described sample intake passage comprises the feed liquor capillary 5, six-way injection valve 12 and the high pressure pump 11 that are communicated with successively with the central through hole 18 of top micro-fluidic chip 1;
Described six-way injection valve 12 ground connection, prevents from getting an electric shock in sample introduction process.
Described buffer solution waste fluid channel comprises the waste liquid capillary 3 and waste liquid bottle 13 that are communicated with successively with the central through hole 18 of top micro-fluidic chip 1;
Described replenisher passage comprises the sheath flow liquid capillary 4 and syringe pump 14 that are communicated with successively with the central through hole 18 of terminal micro-fluidic chip 2;
Described flow pass comprises the transfer capillary 6 be communicated with the central through hole 18 of terminal micro-fluidic chip 2;
Described transfer capillary 6 is connected with plasma mass spectrograph 16 by atomizer 15, heated nebulization chamber 20 successively.
One end of first capillary is communicated with the central through hole 18 of described top micro-fluidic chip sheet 1, and the other end is inserted with high-field electrode 7.
Described first capillary and described waste liquid capillary 3 are separately positioned on the both sides of top micro-fluidic chip 1.
One end of second capillary is communicated with the central through hole 18 of described terminal micro-fluidic chip 2, and the other end is inserted with earth electrode 8.
Described second capillary and described sheath flow liquid capillary 4 are separately positioned on the both sides of the micro-fluidic core 2 of terminal.
Described high-field electrode 7 and earth electrode 8 are all platinum electrodes.
Further, described split channel 17 is uniformly distributed along micro-fluidic chip.
Waste liquid capillary 3 internal diameter is 0.05mm, and external diameter is 0.35mm, and length is 50cm;
Feed liquor capillary 5 internal diameter is 0.05mm, and external diameter is 0.35mm, and length is 50mm;
Sheath flow liquid capillary 4 internal diameter is 0.05mm, and external diameter is 0.35mm, and length is 25cm;
Transfer capillary 6 internal diameter is 0.075mm, and external diameter is 0.35mm, and length is 10cm;
Connection capillary inner diameter is 0.075mm, and external diameter is 0.35mm, and length is 50cm;
Radial dark 30 μm of split channel, wide 100 μm, long 5mm.
Top micro-fluidic chip 1 and terminal micro-fluidic chip 2 are equipped with 17 radial split channels 17, article 17, one end of radial split channel 17 intersects at and central through hole 18, the other end is provided with perforate 20, the diameter of central through hole is 0.35mm, the diameter of perforate 20 is 0.35mm, radial split channel 17 by perforate 20 be connected capillary 9 and be connected, and to be tightly connected gap by epoxide-resin glue.
Transfer capillary 6, sheath flow liquid capillary 4 are all communicated with the central through hole 18 of terminal micro-fluidic chip 2, and to be tightly connected gap by epoxide-resin glue.
Be communicated with feed liquor capillary 5, waste liquid capillary 3 inside the central through hole of top micro-fluidic chip 1, outside is communicated with the first capillary 7, and gap all seals with epoxide-resin glue.
Be communicated with transfer capillary 6, sheath flow liquid capillary 4 inside the central through hole of top micro-fluidic chip 1, outside is communicated with the second capillary 8, and gap all seals with epoxide-resin glue.
The aperture of chip 1 end inserts feed liquor capillary 5, feed liquor capillary 5 is connected with a wherein hole of six-way injection valve 12, the outside of the central through hole of top micro-fluidic chip 1 is communicated with one end of the first capillary, the other end of the first capillary inserts High Voltage Pt electrode 7, the inner side of the central through hole 18 of top micro-fluidic chip 1 is communicated with one end of waste liquid capillary 3, and the other end of waste liquid capillary 3 is connected with waste liquid bottle 13; The outside of the central through hole of terminal micro-fluidic chip 2 is communicated with one end of the second capillary, the other end of the second capillary inserts ground connection platinum electrode 7, the inner side of the central through hole of terminal micro-fluidic chip 2 is communicated with one end of sheath flow liquid capillary 4, the other end of sheath flow liquid capillary 4 and the syringe needle phase downlink connection on syringe pump 14; The two poles of the earth of high voltage source are connected with ground connection platinum electrode 8 with High Voltage Pt electrode 7 respectively.
Transfer capillary 6 is by four fluorine tube seamless link atomizer 15, and atomizer 15 is connected with plasma mass spectrograph 16 again.
Analytic process of the present invention is made up of sample introduction, separation and detection three phases:
(1) the sample introduction stage
The syringe of syringe pump 14 filled sheath flow liquid (1%HNO3) and push away liquid with 5 μ L/min, start high pressure pump with the flow velocity of 60 μ L/min conveying electrophoretic buffer (5mM borax+1mM cysteine, pH8.5) to split tunnel, High Voltage Pt electrode 7 applies+10kV high voltage, ground connection platinum electrode 8 ground connection, three kinds of mercury shape ion (i.e. mercury ions are sucked with tack liquid-phase inlet pin, methyl mercury ion, phenyl mercury ion) mixed sample about 15 μ L, after sample being manually injected into the sample loop (10 μ L) of six-way injection valve 12, manually six-way injection valve 12 is switched to Inject (injection) state, sample in sample loop is injected into through feed liquor capillary 5 and is full of in the split tunnel of electrophoretic buffer by the electrophoretic buffer that high pressure pump 11 is carried.
(2) separation phase
Sample under the acting in conjunction of electric field force and EOF, by each component molecular size, with charge number, electrophoretic velocity the split tunnel that do not coexist in realize being separated; Sample moves to terminal micro-fluidic chip 2 from top micro-fluidic chip 1 through connecting capillary 9, and converges at central through hole 18 place of terminal micro-fluidic chip 2 through the radial split channel 17 of terminal micro-fluidic chip 2, and sample separation is formed.
Sample belt length in radial split channel 17 is equal, and is directly proportional to the sample size of six-way injection valve 12 and high pressure field intensity.
(3) detection-phase
The sample separation converged at central through hole 18 place of terminal micro-fluidic chip 2 enters atomizer 15 by transfer capillary 6 under sheath flow liquid drives, atomization forms aerosol and goes molten in heated nebulization chamber 20, finally enter plasma mass spectrograph 16 and carry out analysis detection, detection obtains
202hg mass number signal strength signal intensity to the electrophoresis peak of time, as shown in Figure 4.
Be provided with 17 radial split channels 17, radial split channel 17 is identical and parallel with one another, sample size, electric-field intensity, electric osmose flow velocity etc. in the radial split channel 17 of every bar are all equal, each radial split channel 17 can carry out electrophoretic separation simultaneously, the total flow of electrophoretic separation is 17 times of the radial split channel of wall scroll, improve the efficiency of electrophoretic separation, and identical sample can be flowed out in the radial split channel 17 of every bar simultaneously, collect after avoiding post and cause electrophoresis peak to widen; Total sample size is 17 times of the radial split channel of wall scroll, and sample total amount increases, and decreases the flow of replenisher, improves the sensitivity that plasma mass detects.
Porous plug 19 is provided with in 17 in the shunting capillary of top micro-fluidic chip 1.The resistance of porous plug 19 pairs of pressure currents is very large, and it is very little to EOF resistance, therefore analyzed sample can under the driving of EOF, enter electrophoretic separation in split tunnel, and the self-priming of atomizer 15 can not form one laminar flow in split tunnel, ensure effectively carrying out of electrophoretic separation on the one hand; The sheath flow liquid that also prevent syringe pump 14 driving on the other hand flows in split tunnel, affects separative efficiency.
Sample molecule after separation converges at central through hole 18 place of terminal micro-fluidic chip 2 through the radial split channel 17 of terminal micro-fluidic chip 2, sample only enters Plasma-Mass Spectroscopy detector 16 by a transfer capillary 6, reduce the dead volume of the interface between electrophoretic separation portion and test section, decrease the time of staying of sample at interface, improve separative efficiency and detection sensitivity.
The present invention adopts sampling valve in conjunction with electrokinetic injection, six-way injection valve 12 is connected with between feed liquor capillary 5 and high pressure pump 11, by tack liquid phase micro-sampling pin, sample is loaded the sample loop of six-way injection valve 12, then the valve position of six-way injection valve 12 is switched to injection (inject) state, high pressure pump 11 carry electrophoretic buffer by the sample injection in quantitative loop in feed liquor capillary 5, and at the central through hole punishment stream at top micro-fluidic chip 1, a part to enter in each shunting capillary in 17 under the effect of pressure current and EOF simultaneously carries out electrophoretic separation, another part then flows out chip through waste liquid capillary 3 and enters in waste liquid bottle.Changing the liquid flowing resistance (as changed the size of waste liquid capillary 3) of waste liquid capillary 3, the sample size entering radial split channel 17 can be regulated, prevent the sample size overload in the radial split channel 17 of wall scroll.The change quantitative loop volume of six-way injection valve 12 and the liquid absorption of tack liquid phase micro-sampling pin, can change the sampling volume entered in radial split channel 17.
Claims (6)
1. a micro-fluidic chip, it is characterized in that: the center of described micro-fluidic chip has central through hole, described micro-fluidic chip take central through hole as the center of circle, at least 2 radial split channels are gone out to fringe radiation, described radial split channel is all identical, and described radial split channel is all communicated with central through hole.
2. a kind of micro-fluidic chip as claimed in claim 1, is characterized in that: described radial split channel is uniformly distributed along described micro-fluidic chip.
3. the electrophoretic separation split tunnel adopting micro-fluidic chip according to claim 1 to build, it is characterized in that: described split tunnel comprises symmetrically arranged two pieces of micro-fluidic chips, be respectively top micro-fluidic chip and terminal micro-fluidic chip, the radial split channel that described top fluidic chip is relative with terminal fluidic chip is all communicated with one by one by being connected capillary, and is equipped with porous plug in the radial split channel of described top micro-fluidic chip.
4. the electrophoretic separation split tunnel adopting micro-fluidic chip according to claim 3 to build, it is characterized in that: the central through hole of top micro-fluidic chip is connected with high-field electrode, the central through hole of terminal micro-fluidic chip is connected with earth electrode, and described high-field electrode and earth electrode are connected with the two ends of high voltage source respectively.
5. the electrophoretic separation split tunnel adopting micro-fluidic chip according to claim 4 to build, is characterized in that: the central through hole place of described top micro-fluidic chip is also connected with feed liquor capillary and waste liquid capillary.
6. the electrophoretic separation split tunnel adopting micro-fluidic chip according to claim 5 to build, is characterized in that: the central through hole place of described top micro-fluidic chip is also connected with sheath flow liquid capillary and transfer capillary.
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| G.YANG ET AL.: "a new interface used to couple capillary electrophoresis with inductively coupled plasma mass spectrometry for speciation analysis", 《ELECTROPHORESIS》 * |
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