CN105412946B - A kind of tumour Glassless nanometer tracer and preparation method thereof - Google Patents
A kind of tumour Glassless nanometer tracer and preparation method thereof Download PDFInfo
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Abstract
The present invention provides a kind of tumour Glassless nanometer tracer, raw material including following proportion number: 5 ~ 50 parts of soluble protein, 40 ~ 300 parts of lecithin, 5 ~ 50 parts of coloring agent, the tumour Glassless nanometer tracer through ultrapure water dissolution, the blue for mixing obtained partial size≤100 nm.Tumour Glassless nanometer tracer of the invention has biological facultative, degradability, it is safe to the human body nontoxic, surgeon is able to achieve under ordinary ray, is not needed by additional instrument, naked eyes distinguish tumor tissues and normal tissue, accurate tumor resection in operation.Tumour Glassless nanometer tracer preparation method in the present invention is suitble to industrialize big rule production.
Description
Technical field
The present invention relates to pharmaceutical technology fields, and in particular to is used for a kind of tumour Glassless nanometer tracer and its preparation
Method.
Background technique
Tumor tissues and normal tissue are easy to distinguish by preoperative plannings such as nuclear magnetic resonance, CT, ultrasounds, but in surgical procedure
In, it is visually difficult to distinguish them.General operation is difficult to cut off cancerous issue completely, leaves hidden danger to disease relapse, if expanded
Excision extension, and normal tissue can be injured, it may result in the sequelae of body function, function etc..Therefore it distinguishes normal
Cell and tumor tissues are always the difficulties of related fields.
The image technologies such as previous MRI, CT, ultrasound need special instrument, it is difficult to realize and directly observe tumour；Tumour is glimmering
Light decoration method needs in darkroom or under darker light, and tumour cell could be distinguished by using up excitation, and dark operating environment is to hand
Art operation brings great inconvenience；Operation guiding system is based on the medical image datas such as nuclear magnetic resonance, CT, in computer
On show " the virtual human brain " of a three-dimensional visible, the probe in doctor's hand be directed toward where, if having arrived at borderline tumor,
Front is vital tissue, can be shown by screen.But for precisely performing the operation, doctor needs to observe pathological tissues,
Computer screen is observed again, constantly toggles visual angle, it is easy to cause operation mistake；General operation guiding system price is high
Expensive, general basic hospital is also difficult to be equipped with；Corresponding Operation Fee also greatly increases, and general disease is difficult to bear.
In order to solve the above problem, Daniel Orringer et al., which has been invented, uses synthetic material --- polypropylene nano grain
For carrier, wrapping up the synthetic dyestuffs such as Coomassie brilliant blue, methylenum careuleum, indocyanine green is tracer, has invented a kind of brain tumor naked eye and has shown
Track agent, it is this using polyacrylamide as in the cancer Glassless image technology of carrier, polypropylene amine is difficult to degrade in vivo, deposits
In bio-compatibility problem, and the synthetic dyestuffs such as Coomassie brilliant blue have certain toxic side effect.
Liposome (liposome) is a kind of artificial membrane.In water in phospholipid molecule hydrophilic head insertion water, liposome is dredged
Air is stretched in water tail portion, and the spherical liposomes of the double-deck rouge molecule are formed after agitation.Liposome includes: that (1) is anti-as pharmaceutical carrier
The carrier of tumour medicine；(2) antibacterial/anti-infectives carrier；(3) carrier of anti-parasite medicine；(4) other aspects.It receives
The conventional preparation techniques of Mizhi plastid: (1) film dispersion method, Cao Jingci et al. prepare ciclosporin A nano-lipid using this method
Body, partial size 104.85nm.(2) injection method, Wu Yani et al. prepare Nano liposome of papain using this method, put down
Equal partial size 100nm.(3) reverse phase evaporation, sieve amber victory et al. prepare Nano Volatile Oil of Houttuynia cordata Thunb. Liposome, partial size using this method
For 90nm.(4) freeze-drying, Zhang Xiaowei et al. prepare tanshinone IIA nano liposomes using this method, and partial size is in 117nm
Left and right.That there are stability is poor for the liposome that above method obtains, and containing exogenous cholesterol or steroid substances, and is difficult to advise greatly
The industrial defect of modelling.
Nano liposomes are a kind of ideal pharmaceutical carriers, receive the attention of domestic and international relevant industries.Currently, having had
It is multiple at home and abroad to be listed by the drug of carrier of nano liposomes, and nano liposomes are also obtained in other medical fields
Application, such as application in directions such as gene therapy, contrast agent.It can be seen that before nano liposomes have good application
Scape.But there are limitations for nano liposomes: stability is poor, it is difficult to large-scale industrial production.Especially be often used cholesterol,
Cholate reduces partial size, enhances stability, thus the external cholesterol of bring especially gives heart pipe Disease band to patient
Carry out toxic side effect.
Summary of the invention
In order to reduce the toxic side effect of tumour Glassless nanometer tracer, increase biological facultative, degradability, raising group
It knits penetrance, realizes surgeon under ordinary ray, do not need by additional instrument, naked eyes distinguish tumor tissues and normal
Tissue, accurate tumor resection in operation, the present invention provide a kind of tumour Glassless nanometer tracer and preparation method thereof, the party
The tumour Glassless of the blue of partial size≤100 nm is made through mixing using soluble protein, lecithin, coloring agent as raw material for method
Nanometer tracer.
The scheme of the invention is realize in this way:
A kind of tumour Glassless nanometer tracer, the raw material including following proportion number: 5 ~ 50 parts of soluble protein, lecithin
40 ~ 300 parts of rouge, 5 ~ 50 parts of coloring agent, the tumour naked eye through ultrapure water dissolution, the blue for mixing obtained partial size≤100 nm can
Depending on nanometer tracer.
Soluble protein refers to can be dissolved in water or the albumen of other solvents with small molecule state.Smudge cells are generally referred to remove
After removing big insoluble particles, also in the albumen of supernatant fraction after ultracentrifugation.Soluble protein is important osmotic field
Matter and nutriment, their increase and accumulation can improve the water holding capacity of cell, and the living matter and biomembrane to cell rise
To protective effect, therefore it is commonly used as one of the index of screening resistance.
The soluble protein is water soluble animal albumen, water-soluble plant albumen, water-soluble mycoprotein, water-soluble thin
One of the water-soluble recombinant protein or more than one compositions of mycoprotein, bacterial expression.
The water soluble animal albumen is seralbumin, globulin, egg protein, casein, Corbicula fluminea water-solubility protein, silkworm
Pupa water-solubility protein, sericin, albumen, ovalbumin etc..
The water-soluble plant albumen is water-soluble soybean protein, semen sojae atricolor albumen, rice protein, zein, wheat
Albumen, ginseng water-soluble protein etc..
The water solubility bacterium or mycoprotein are water-soluble for water-soluble ganoderma lucidum albumen, water solubility hedgehog hydnum mycoprotein, rhizobium
Property albumen etc..
The water-soluble recombinant protein of the bacterial expression is the solubility of genetic recombination human serum albumin, Bacillus coli expression
Albumen etc..
The coloring agent is Biological cross-linker or medicinal methylene blue derivative or their composition.
The Biological cross-linker is from mast, olive growing leaves, honeysuckle cauline leaf, the fruit of glossy privet, oldenlandia diffusa plant extract
Natural iridoid glycoside compound and its hydrolysis derivative.
The natural iridoid glycoside compound and its hydrolysis derivative are Geniposide GP, loganin aglycon LA, olive are bitter
One of glycosides aglycon OA, E-6-O coumaric acyl Paederia scandens time glycosides methyl esters aglycon EA or two kinds of composition.
The medicinal methylene blue derivative refers to the methylenum careuleum double activated ester derivant having the following structure:
Wherein R1,R2, it is hydrogen atom or sodium sulfonate, X is halide ion or is sulfate radical, nitrate anion, n=1-5.
Such as the medicinal methylene blue derivative is methylenum careuleum dipropionic acid NHS ester, methylenum careuleum succinic acid NHS rouge, methylenum careuleum
Dipropionic acid N- hydroxy thiosuccinimide ester, it is a kind of in methylenum careuleum succinic acid N- hydroxy thiosuccinimide ester or containing with
The composition of one or more of the derivative of upper structure.
The method for preparing above-mentioned tumour Glassless nanometer tracer, comprising the following steps:
(1) 40 ~ 300 parts of lecithin are added in the nonpolar solvent of energy dissolving lecithin, lecithin is made in stirring and dissolving
Lipoprotein solution；
(2) 5-50 parts of coloring agents are added in ultrapure water, dyeing agent solution is made in stirring and dissolving；
(3) 5 ~ 50 parts of soluble proteins are added in ultrapure water, protein solution is made in stirring and dissolving, obtains with step (1)
The lecithin soln arrived is mixed by the volume ratio of 1 ~ 2:15, and albumen lecithin mixed liquor is made；
(4) by the albumen lecithin mixed liquor that step (3) obtains and the dyeing agent solution that step (2) obtain by 15:1 ~ 2
Volume ratio mixes, and traced fluid is made；
(5) traced fluid that step (4) obtains is stirred at normal temperature, then is evaporated under reduced pressure the non-pole except the dissolving lecithin that deenergizes
Property solvent, obtains blue milkiness shape traced fluid；
(6) 7.5 times of ultrapure waters with upper volume are added into the blue milkiness shape traced fluid that step (5) obtains, stir molten
Solution, is made traced fluid dilution；
(7) it by above-mentioned traced fluid dilution after -20 ~ -80 DEG C or Liquid nitrogen precooler freeze, then is freeze-dried, obtains partial size
The tumour Glassless nanometer tracer of the blue of≤100 nm.
The concentration of the lecithin soln is 1.6 ~ 10%；The concentration of step (4) traced fluid is 7 ~ 20%；The albumen
The concentration of matter solution is 1 ~ 10%；It is described can dissolving lecithin nonpolar solvent be hexamethylene, n-hexane, normal heptane, boiling point are
One of 50 ~ 90 DEG C of petroleum ether, chloroform, methylene chloride or more than one compositions.
The method of the mixing is concussion ultrasonic time 10min or more or homogenizer homogeneous 8min or more or high speed dispersion
Machine disperses 10min or more.
The coloring agent is Biological cross-linker or medicinal methylene blue derivative or their composition, the soluble protein
For water soluble animal albumen, water-soluble plant albumen, water-soluble mycoprotein, water-soluble bacterioprotein, bacterial expression it is water-soluble
One of property recombinant protein or more than one compositions.
The Biological cross-linker is from mast, olive growing leaves, honeysuckle cauline leaf, the fruit of glossy privet, oldenlandia diffusa plant extract
Natural iridoid glycoside compound and its hydrolysis derivative.
The natural iridoid glycoside compound and its hydrolysis derivative are Geniposide GP, loganin aglycon LA, olive are bitter
One of glycosides aglycon OA, E-6-O coumaric acyl Paederia scandens time glycosides methyl esters aglycon EA or two kinds of composition.
The medicinal methylene blue derivative refers to the methylenum careuleum double activated ester derivant having the following structure:
Wherein R1, R2, are hydrogen atom or sodium sulfonate, and X is halide ion or is sulfate radical, nitrate anion, n=1-5.
Such as the medicinal methylene blue derivative is methylenum careuleum dipropionic acid NHS ester, methylenum careuleum succinic acid NHS rouge, methylenum careuleum
Dipropionic acid N- hydroxy thiosuccinimide ester, it is a kind of in methylenum careuleum succinic acid N- hydroxy thiosuccinimide ester or containing with
The composition of one or more of the derivative of upper structure.
Beneficial effect
1, tumour Glassless nanometer tracer partial size≤100 nm of the invention readily penetrate through biomembrane so having
The ability of equal physiological tissues' barrier, especially its surface have many active groups, such as amino, carboxyl, hydroxyl, easily special with tumour
The coupling of the small molecules such as anisotropic target polypeptide, cell-penetrating peptide, makes it have collaboration targeting, it can quickly reach tumor group
It knits, and is swallowed by tumour cell, since tumour Glassless nanometer tracer has color, tumor tissues are labeled, medical care people
Member is just it can be found that tumor tissues and quickly cut off；In addition, tumour Glassless nanometer tracer partial size is small, the population of formation
The atom number on the surface layer of amount and the nanoparticle formed significantly increases, and ability, the ability of attachment covered obtain
Reinforce, the contact area of they and cell will greatly increase, by faster more stable and contact tumor tissue.
2, tumour Glassless nanometer tracer of the invention does not need special operating room, does not need by additional yet
Instrument, in normal light and common operating room, medical staff can visually distinguish tumor tissues and normal tissue, operation
In accurate tumor resection, i.e., do not leave future trouble and do not injure normal tissue.
3, tumour Glassless nanometer tracer of the invention is using the Biological cross-linker or medicinal Asia being extracted from plants
First indigo plant derivative replaces the stabilizer of cholesterol and its derivative as liposome, avoids the use of cholesterol, avoids gallbladder
Sterol causes the side effect of artery sclerosis, coronary heart disease etc. to human body.
4, tumour Glassless nanometer tracer of the invention has biological facultative, degradability, safe to the human body nontoxic.
5, tumour Glassless nanometer tracer stability of the invention is good, and after placing half a year, partial size is basically unchanged.
6, tumour Glassless nanometer tracer raw material of the invention is simple and easy to get, and preparation method is easy, can be extensive
Chemical industry production；Low in cost, hospital can be applicable in, and application is wide.
Detailed description of the invention
Fig. 1: for 1 tumour Glassless nanometer tracer transmission electron microscope picture of embodiment, partial size 80nm；
Fig. 2: being 3 tumour Glassless nanometer tracer partial size of embodiment with freezing time variation diagram；
Fig. 3: being 4 tumour Glassless nanometer tracer of embodiment to breast cancer MDA-NB-231 cell strain mouse model
Dyeing effect, arrow meaning is the tumor tissues blue by dye；
Fig. 4: 5 tumour Glassless nanometer tracer of embodiment cuts C6 glioma SD rat model tracer effect video
Figure, it is left: before tracer, can not to find out tumor tissues, right: after tracer, tumor tissues and diffusion zone are dyed to blue.
Specific embodiment
Tumour Glassless nanometer tracer of the present invention is described further below by embodiment.
The key instrument device name model that the present invention uses is exemplified below, but not limited to this model:
Drug: lecithin, the nonpolar solvent of energy dissolving lecithin, soluble protein, Biological cross-linker, medicine methylenum careuleum are spread out
Biologies etc. are commercial product or pass through further purified product, and ultrapure water preparation is the ultrapure water generated by the ultrapure device in Nanjing forward position
It is obtained using the sterile intake of milipore ultrapure water.
One, embodiment is prepared
Embodiment 1
A kind of tumour Glassless nanometer tracer, the raw material including following proportion number:
Soluble protein: seralbumin 5mg；
Lecithin: 40mg；
Coloring agent: Geniposide GP5 mg；
The method for preparing above-mentioned tumour Glassless nanometer tracer, comprising the following steps:
(1) lecithin is added in hexamethylene, stirring and dissolving, the lecithin soln that concentration is 1.6% is made；
(2) coloring agent is added in ultrapure water, stirring and dissolving, the dyeing agent solution that concentration is 7% is made；
(3) soluble protein is added in ultrapure water, stirring and dissolving, the protein solution that concentration is 1% is made, with step
(1) lecithin soln obtained is mixed by the volume ratio of 1:15, shakes ultrasound 12min, albumen lecithin mixed liquor is made；
(4) the albumen lecithin mixed liquor that step (3) obtains is dyed into agent solution by the body of 15:1 with what step (2) obtained
Product shakes ultrasound 12min, traced fluid is made than mixing；
(5) traced fluid that step (4) obtains is stirred at normal temperature, then is evaporated under reduced pressure removing hexamethylene, obtain blue cream
Turbid shape traced fluid；
(6) water of 7.5 times of volumes is added into the blue milkiness shape traced fluid that step (5) obtains, ultrasonic dissolution is made and shows
Track liquid dilution；
(7) it by above-mentioned traced fluid dilution in -20 DEG C of precoolings, then is freeze-dried, obtains the blue that partial size is 80 nm
Tumour Glassless nanometer tracer.
Embodiment 2
A kind of tumour Glassless nanometer tracer, the raw material including following proportion number:
Soluble protein: 50 mg of rice protein；
Lecithin: 300mg；
Coloring agent: 50 mg of methylenum careuleum dipropionic acid NHS ester；
The method for preparing above-mentioned tumour Glassless nanometer tracer, comprising the following steps:
(1) lecithin is added in n-hexane, stirring and dissolving, the lecithin soln that concentration is 10% is made；
(2) coloring agent is added in ultrapure water, stirring and dissolving, the dyeing agent solution that concentration is 20% is made；
(3) soluble protein is added in ultrapure water, stirring and dissolving, the protein solution that concentration is 10% is made, with step
(1) lecithin soln obtained is put into homogenizer homogeneous 10min by the volume ratio of 2:15, and albumen lecithin mixed liquor is made；
(4) the albumen lecithin mixed liquor that step (3) obtains is dyed into agent solution by the body of 15:2 with what step (2) obtained
Product ratio is put into homogenizer homogeneous 10min, and traced fluid is made；
(5) traced fluid that step (4) obtains is stirred at normal temperature, then is evaporated under reduced pressure removing n-hexane, obtain blue cream
Turbid shape traced fluid；
(6) water of 20 times of volumes is added into the blue milkiness shape traced fluid that step (5) obtains, ultrasonic dissolution is made and shows
Track liquid dilution；
(7) it by above-mentioned traced fluid dilution in -80 DEG C of precoolings, then is freeze-dried, obtains the blue that partial size is 72 nm
Tumour Glassless nanometer tracer.
Embodiment 3
A kind of tumour Glassless nanometer tracer, the raw material including following proportion number:
Soluble protein: 30 mg of silkworm chrysalis water-solubility protein, 10 mg of soybean protein；
Lecithin: 200 mg；
Coloring agent: 15 mg of methylenum careuleum succinic acid NHS rouge, 5 mg of loganin aglycon LA；
The method for preparing above-mentioned tumour Glassless nanometer tracer, comprising the following steps:
(1) lecithin is added in normal heptane, stirring and dissolving, the lecithin soln that concentration is 8% is made；
(2) coloring agent is added in ultrapure water, stirring and dissolving, the dyeing agent solution that concentration is 12% is made；
(3) soluble protein is added in ultrapure water, stirring and dissolving, the protein solution that concentration is 5% is made, with step
(1) lecithin soln obtained is put into high speed disperser dispersion 15min by the volume ratio of 1:15, and the mixing of albumen lecithin is made
Liquid；
(4) the albumen lecithin mixed liquor that step (3) obtains is dyed into agent solution by the body of 15:2 with what step (2) obtained
Traced fluid is made than being put into high speed disperser dispersion 15min in product；
(5) traced fluid that step (4) obtains is stirred at normal temperature, then is evaporated under reduced pressure removing normal heptane, obtain blue cream
Turbid shape traced fluid；
(6) water of 10 times of volumes is added into the blue milkiness shape traced fluid that step (5) obtains, ultrasonic dissolution is made and shows
Track liquid dilution；
(7) it by above-mentioned traced fluid dilution in -60 DEG C of precoolings, then is freeze-dried, obtains the blue that partial size is 77 nm
Tumour Glassless nanometer tracer.
Embodiment 4
A kind of tumour Glassless nanometer tracer, the raw material including following proportion number:
Soluble protein: globulin 2mg, egg protein 3mg, casein 2mg, genetic recombination human serum albumin 3mg, semen sojae atricolor egg
White 5 mg, 5 mg of sericin；
Lecithin: 100 mg；
Coloring agent: 20 mg, E-6-O coumaric acyl Paederia scandens time glycosides of methylenum careuleum dipropionic acid N- hydroxy thiosuccinimide ester
Methyl esters aglycon EA10mg；
The method for preparing above-mentioned tumour Glassless nanometer tracer, comprising the following steps:
(1) lecithin is added in methylene chloride, stirring and dissolving, the lecithin soln that concentration is 8% is made；
(2) coloring agent is added in ultrapure water, stirring and dissolving, the dyeing agent solution that concentration is 15% is made；
(3) soluble protein is added in ultrapure water, stirring and dissolving, the protein solution that concentration is 7% is made, with step (1)
Obtained lecithin soln is mixed by the volume ratio of 2:15, shakes ultrasound 15min, albumen lecithin mixed liquor is made；
(4) the albumen lecithin mixed liquor that step (3) obtains is dyed into agent solution by the body of 15:1 with what step (2) obtained
Product shakes ultrasound 15min, traced fluid is made than mixing；
(5) traced fluid that step (4) obtains is stirred at normal temperature, then is evaporated under reduced pressure removing methylene chloride, obtain blue
Milkiness shape traced fluid；
(6) water of 30 times of volumes is added into the blue milkiness shape traced fluid that step (5) obtains, ultrasonic dissolution is made and shows
Track liquid dilution；
(7) it by above-mentioned traced fluid dilution precooling in liquid nitrogen, then is freeze-dried, obtains the indigo plant that partial size is 68 nm
The tumour Glassless nanometer tracer of color.
Embodiment 5
A kind of tumour Glassless nanometer tracer, the raw material including following proportion number:
Soluble protein: 5 mg of ovalbumin, wheat gluten 5mg, the soluble protein 5mg of Bacillus coli expression, ganoderma lucidum
Water-solubility protein 5mg, ginseng water-soluble protein 5mg；
Lecithin: 50 mg；
Coloring agent: 30 mg of methylenum careuleum succinic acid N- hydroxy thiosuccinimide ester, methylenum careuleum dipropionic acid NHS ester 5g, E-
2 mg of 6-O coumaric acyl Paederia scandens time glycosides methyl esters aglycon EA, 5 mg of Oleuropeine aglycone OA；
The method for preparing above-mentioned tumour Glassless nanometer tracer, comprising the following steps:
(1) by lecithin addition hexamethylene, n-hexane, the lecithin soln that concentration is 5% is made in stirring and dissolving；
(2) coloring agent is added in ultrapure water, stirring and dissolving, the dyeing agent solution that concentration is 10% is made；
(3) soluble protein is added in ultrapure water, stirring and dissolving, the protein solution that concentration is 3% is made, with step
(1) lecithin soln obtained is mixed by the volume ratio of 2:15, shakes ultrasound 12min, albumen lecithin mixed liquor is made；
(4) the albumen lecithin mixed liquor that step (3) obtains is dyed into agent solution by the body of 15:2 with what step (2) obtained
Product shakes ultrasound 12min, traced fluid is made than mixing；
(5) traced fluid that step (4) obtains is stirred at normal temperature, then is evaporated under reduced pressure and removes hexamethylene, n-hexane, obtained
Blue milkiness shape traced fluid；
(6) water of 25 times of volumes is added into the blue milkiness shape traced fluid that step (5) obtains, ultrasonic dissolution is made and shows
Track liquid dilution；
(7) it by above-mentioned traced fluid dilution in -40 DEG C of precoolings, then is freeze-dried, obtains the blue that partial size is 73 nm
Tumour Glassless nanometer tracer.
Embodiment 6
A kind of tumour Glassless nanometer tracer, the raw material including following proportion number:
Soluble protein: water-soluble ganoderma lucidum protein 18 mg；
Lecithin: 200 mg；
Coloring agent: 10 mg of methylenum careuleum dipropionic acid NHS ester, methylenum careuleum dipropionic acid NHS ester 5g, E-6-O coumaric acyl Paederia scandens
5 mg of glycosides methyl esters aglycon EA, 5 mg of Oleuropeine aglycone OA；
The method for preparing above-mentioned tumour Glassless nanometer tracer, comprising the following steps:
(1) lecithin is added in n-hexane, stirring and dissolving, the lecithin soln that concentration is 9% is made；
(2) coloring agent is added in ultrapure water, stirring and dissolving, the dyeing agent solution that concentration is 18% is made；
(3) soluble protein is added in ultrapure water, stirring and dissolving, the protein solution that concentration is 7% is made, with step (1)
Obtained lecithin soln is mixed by the volume ratio of 1:15, and albumen lecithin mixed liquor is made in homogenizer homogeneous 12min；
(4) the albumen lecithin mixed liquor that step (3) obtains is dyed into agent solution by the body of 15:2 with what step (2) obtained
Traced fluid is made than mixing, homogenizer homogeneous 12min in product；
(5) traced fluid that step (4) obtains is stirred at normal temperature, then is evaporated under reduced pressure removing n-hexane, obtain blue cream
Turbid shape traced fluid；
(6) water of 18 times of volumes is added into the blue milkiness shape traced fluid that step (5) obtains, ultrasonic dissolution is made and shows
Track liquid dilution；
(7) it by above-mentioned traced fluid dilution in -50 DEG C of precoolings, then is freeze-dried, obtains the blue that partial size is 69 nm
Tumour Glassless nanometer tracer.
Embodiment 7
A kind of tumour Glassless nanometer tracer, the raw material including following proportion number:
Soluble protein: sericin 10mg, water-soluble Hericium erinaceus protein 12 mg, genetic recombination human serum albumin 5mg；
Lecithin: 220 mg；
Coloring agent: 10 mg, E-6-O coumaric acyl Paederia scandens time glycosides methyl esters aglycon EA of methylenum careuleum dipropionic acid NHS ester, 15 mg,
5 mg of Geniposide GP；
The method for preparing above-mentioned tumour Glassless nanometer tracer, comprising the following steps:
(1) lecithin is added in hexamethylene, stirring and dissolving, the lecithin soln that concentration is 5% is made；
(2) coloring agent is added in ultrapure water, stirring and dissolving, the dyeing agent solution that concentration is 10% is made；
(3) soluble protein is added in ultrapure water, stirring and dissolving, the protein solution that concentration is 3% is made, with step (1)
Obtained lecithin soln is mixed by the volume ratio of 2:15, and high speed disperser disperses 18min, and albumen lecithin mixed liquor is made；
(4) the albumen lecithin mixed liquor that step (3) obtains is dyed into agent solution by the body of 15:1 with what step (2) obtained
Product disperses 18min, traced fluid is made than mixing, high speed disperser；
(5) traced fluid that step (4) obtains is stirred at normal temperature, then is evaporated under reduced pressure removing hexamethylene, obtain blue cream
Turbid shape traced fluid；
(6) water of 35 times of volumes is added into the blue milkiness shape traced fluid that step (5) obtains, ultrasonic dissolution is made and shows
Track liquid dilution；
(7) it by above-mentioned traced fluid dilution in -20 DEG C of precoolings, then is freeze-dried, obtains the blue that partial size is 75 nm
Tumour Glassless nanometer tracer.
Embodiment 8
A kind of tumour Glassless nanometer tracer, the raw material including following proportion number:
Soluble protein: zein 10mg, albumen 2mg, genetic recombination human serum albumin 5mg；
Lecithin: 60 mg；
Coloring agent: 10 mg of methylenum careuleum dipropionic acid NHS ester 5g, E-6-O coumaric acyl Paederia scandens time glycosides methyl esters aglycon EA, olive
Bitter 5 mg of glycosides aglycon OA；
The method for preparing above-mentioned tumour Glassless nanometer tracer, comprising the following steps:
(1) lecithin is added in normal heptane, stirring and dissolving, the lecithin soln that concentration is 6% is made；
(2) coloring agent is added in ultrapure water, stirring and dissolving, the dyeing agent solution that concentration is 16% is made；
(3) protein is added in ultrapure water, stirring and dissolving, the protein solution that concentration is 4% is made, is obtained with step (1)
Lecithin soln by 1:15 volume ratio mix, shake ultrasound 15min, albumen lecithin mixed liquor is made；
(4) the albumen lecithin mixed liquor that step (3) obtains is dyed into agent solution by the body of 15:1 with what step (2) obtained
Product shakes ultrasound 15min, traced fluid is made than mixing；
(5) traced fluid that step (4) obtains is stirred at normal temperature, then is evaporated under reduced pressure removing normal heptane, obtain blue cream
Turbid shape traced fluid；
(6) water of 20 times of volumes is added into the blue milkiness shape traced fluid that step (5) obtains, ultrasonic dissolution is made and shows
Track liquid dilution；
(7) it by above-mentioned traced fluid dilution in -60 DEG C of precoolings, then is freeze-dried, obtains the blue that partial size is 70 nm
Tumour Glassless nanometer tracer.
Two, Application Example
Application method: adding ultrapure water for tumour Glassless nanometer tracer, ultrasonic dissolution, can carry out using.
Embodiment one
The breast cancer MBA-MB-231 cell for being in logarithmic growth phase is collected, single cell suspension is made, takes 1 × 107,
0.2mL cell inoculation is subcutaneous in nude mice oxter.Start tumor formation after 2 weeks, is put to death when tumour growth to diameter l cm size after 3 weeks
Tumor bearing nude mice, removing knurl is put into physiological saline under aseptic condition, rejects coating and connective tissue, tumor tissues are cut into 2
Mm × 2 mm × 2mm size is then inoculated with 10 female nude mices.About 1.0 cm of chest wall cutaneous on the right side of nude mice is cut,
Tumor tissue is seeded on the right side of nude mice in the fat pad of second pair of mammary gland, sews up the incision by second pair of mammary fat pad of exposure.
After 12 days, adds water ultrasonic dissolution to carry out nipple injection tumour Glassless nanometer tracer described in embodiment 4, after disinfection, take
Mammary gland observes tracer effect.Tracer effect as shown in figure 3, tracer to breast cancer MDA-NB-231 cell strain mouse model
Dyeing effect, arrow meaning are the tumor tissues blue by dye.
Embodiment two
The preoperative fasting in 12 hours of SD rat, after anesthesia, cuts off crown hair, fixed, routine disinfection, and head clearing reveals skull,
Aperture is bored, endocranium can not be injured.Micro syringe extracts the C6 cell suspension of 25ul, and needle point touches endocranium, along drill into
3.5 mm of needle retreats 1 mm and slowly injects cell suspension, and the slow withdraw of the needle closes bone hole with bacteria-free bone wax, and peace and quiet visual area sterilizes,
Tumour Glassless nanometer tracer described in embodiment 5 after conventinal breeding 12 days, is added water ultrasonic by skin suture, notch disinfection
End of line intravenous injection is dissolved into, cranium observation tracer effect is opened after 2 hours, after another group of injecting normal saline, does control experiment.Show
Track effect is as shown in Figure 4.C6 glioma SD rat model tracer effect video screenshot.It is left: before tracer, can not to find out tumour
Tissue, right: after tracer, tumor tissues and diffusion zone are dyed to blue.
Claims (6)
1. a kind of tumour Glassless nanometer tracer, which is characterized in that by the raw material of following proportion number: soluble protein 5 ~ 50
Part, 40 ~ 300 parts of lecithin, 5 ~ 50 parts of coloring agent, the tumour through ultrapure water dissolution, the blue for mixing obtained partial size≤100 nm
Glassless nanometer tracer；
The coloring agent is Biological cross-linker or methylene blue derivatives or their composition, and the soluble protein is water solubility
Animal protein, water-soluble plant albumen, water-soluble mycoprotein, water-soluble bacterioprotein, the water-soluble of bacterial expression recombinate egg
One of white or more than one compositions；
The Biological cross-linker be from mast, olive growing leaves, honeysuckle cauline leaf, the fruit of glossy privet, oldenlandia diffusa plant extract it is natural
Iridoid glycoside compound and its hydrolysis derivative；
The natural iridoid glycoside compound and its hydrolysis derivative are Geniposide GP, loganin aglycon LA, oleuropein glycosides
One of first OA, E-6-O coumaric acyl Paederia scandens time glycosides methyl esters aglycon EA or two kinds of composition；
The methylene blue derivatives refer to the methylenum careuleum dipropionic acid NHS ester with methylenum careuleum double activated ester derivant, methylenum careuleum fourth
Diacid NHS rouge, methylenum careuleum dipropionic acid N- hydroxy thiosuccinimide ester, methylenum careuleum succinic acid N- hydroxy thiosuccinimide
The composition of one or more of a kind of or derivative containing the above structure in ester.
2. the preparation method of tumour Glassless nanometer tracer according to claim 1, which is characterized in that including following step
It is rapid:
(1) 40 ~ 300 parts of lecithin are added in the nonpolar solvent of energy dissolving lecithin, lecithin liposoluble is made in stirring and dissolving
Liquid；
(2) 5-50 parts of coloring agents are added in ultrapure water, dyeing agent solution is made in stirring and dissolving；
(3) 5 ~ 50 parts of soluble proteins are added in ultrapure water, protein solution is made in stirring and dissolving, the ovum obtained with step (1)
Phospholipid solution is mixed by the volume ratio of 1 ~ 2:15, and albumen lecithin mixed liquor is made；
(4) by albumen lecithin mixed liquor that step (3) obtains and the dyeing agent solution that step (2) obtain by the volume of 15:1 ~ 2
Than mixing, traced fluid is made；
(5) traced fluid that step (4) obtains is stirred at normal temperature, then be evaporated under reduced pressure except the nonpolarity for the dissolving lecithin that deenergizes is molten
Agent obtains blue milkiness shape traced fluid；
(6) 7.5 times of ultrapure waters with upper volume, stirring and dissolving, system are added into the blue milkiness shape traced fluid that step (5) obtains
At traced fluid dilution；
(7) it by above-mentioned traced fluid dilution after -20 ~ -80 DEG C or Liquid nitrogen precooler freeze, then is freeze-dried, obtains partial size≤100
The tumour Glassless nanometer tracer of the blue of nm；
The concentration of the lecithin soln is 1.6 ~ 10%；The concentration of the traced fluid is 7 ~ 20%；The concentration of the protein solution
It is 1 ~ 10%；The petroleum ether, chloroform, dichloro that the nonpolar solvent is hexamethylene, n-hexane, normal heptane, boiling point are 50 ~ 90 DEG C
One of methane or more than one compositions.
3. the preparation method of tumour Glassless nanometer tracer according to claim 2, which is characterized in that the mixing
Method be concussion ultrasonic time 10min or more or homogenizer homogeneous 8min or more or high speed disperser disperse 10min or more.
4. the preparation method of tumour Glassless nanometer tracer according to claim 2, it is characterised in that: the dyeing
Agent is Biological cross-linker or methylene blue derivatives or their composition, and the soluble protein is water soluble animal albumen, water
Soluble plant albumen, water-soluble mycoprotein, water-soluble bacterioprotein, bacterial expression one of water-soluble recombinant protein or
More than one compositions.
5. the preparation method of tumour Glassless nanometer tracer according to claim 2, it is characterised in that: the biology
Crosslinking agent be from mast, olive growing leaves, honeysuckle cauline leaf, the fruit of glossy privet, oldenlandia diffusa plant extract natural iridoid glycosidation
Close object and its hydrolysis derivative.
6. a kind of tumour Glassless nanometer tracer, which is characterized in that be made of the raw material of following proportion number:
Soluble protein: sericin 10mg, water-soluble Hericium erinaceus protein 12 mg, genetic recombination human serum albumin 5mg；
Lecithin: 220 mg；
Coloring agent: 10 mg, E-6-O coumaric acyl Paederia scandens time glycosides methyl esters aglycon EA of methylenum careuleum dipropionic acid NHS ester, 15 mg, capital Buddhist nun
Flat 5 mg of GP；
Steps are as follows for preparation method:
(1) lecithin is added in hexamethylene, stirring and dissolving, the lecithin soln that concentration is 5% is made；
(2) coloring agent is added in ultrapure water, stirring and dissolving, the dyeing agent solution that concentration is 10% is made；
(3) soluble protein is added in ultrapure water, stirring and dissolving, the protein solution that concentration is 3% is made, then obtain with step (1)
The lecithin soln arrived is mixed by the volume ratio of 2:15, and high speed disperser disperses 18min, and albumen lecithin mixed liquor is made；
(4) the albumen lecithin mixed liquor that step (3) obtains is dyed into agent solution by the volume ratio of 15:1 with what step (2) obtained
Mixing, high speed disperser disperse 18min, traced fluid are made；
(5) traced fluid that step (4) obtains is stirred at normal temperature, then is evaporated under reduced pressure removing hexamethylene, obtain blue milkiness shape
Traced fluid；
(6) water of 35 times of volumes is added into the blue milkiness shape traced fluid that step (5) obtains, traced fluid is made in ultrasonic dissolution
Dilution；
(7) the traced fluid dilution for obtaining step (6) is in -20 DEG C of precoolings, then is freeze-dried, and the tumour for obtaining blue is naked
The visual nanometer tracer of eye.
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| "Hydrogel Nanoparticles with Covalently Linked Coomassie Blue for Brain Tumor Delineation Visible to the Surgeon";Guochao Nie等;《small》;20121231(第6期);第884-891页 |
| "淋巴造影剂专利蓝脂质体的制备及其生物学表征";马建忠等;《国际外科学杂志》;20100630;第37卷(第6期);标题，第380页右栏，第380页左栏第1段，第381页右栏第2段 |
| "脂质体中蛋白类药物包封率的测定";李可欣等;《沈阳药科大学学报》;20100930;第27卷(第9期);第680页右栏第1段 |
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