CN105412318A - Traditional Chinese medicine fumigation and washing bag soaking agent for treating knee osteoarthritis and preparation method thereof - Google Patents

Traditional Chinese medicine fumigation and washing bag soaking agent for treating knee osteoarthritis and preparation method thereof Download PDF

Info

Publication number
CN105412318A
CN105412318A CN201511028162.7A CN201511028162A CN105412318A CN 105412318 A CN105412318 A CN 105412318A CN 201511028162 A CN201511028162 A CN 201511028162A CN 105412318 A CN105412318 A CN 105412318A
Authority
CN
China
Prior art keywords
parts
chinese medicine
preparation
drug
radix
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201511028162.7A
Other languages
Chinese (zh)
Inventor
苏友新
张英杰
郑良朴
盛录
李亚楠
卢美丽
刘长艳
王艺茹
王雯婷
何浈
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujian University of Traditional Chinese Medicine
Original Assignee
Fujian University of Traditional Chinese Medicine
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fujian University of Traditional Chinese Medicine filed Critical Fujian University of Traditional Chinese Medicine
Priority to CN201511028162.7A priority Critical patent/CN105412318A/en
Publication of CN105412318A publication Critical patent/CN105412318A/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A61K36/286—Carthamus (distaff thistle)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/11—Pteridophyta or Filicophyta (ferns)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/11—Pteridophyta or Filicophyta (ferns)
    • A61K36/12—Filicopsida or Pteridopsida
    • A61K36/126—Drynaria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A61K36/232—Angelica
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A61K36/236—Ligusticum (licorice-root)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A61K36/282—Artemisia, e.g. wormwood or sagebrush
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/47—Euphorbiaceae (Spurge family), e.g. Ricinus (castorbean)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
    • A61K36/714—Aconitum (monkshood)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
    • A61K36/716—Clematis (leather flower)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/75—Rutaceae (Rue family)
    • A61K36/758—Zanthoxylum, e.g. pricklyash
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0014—Skin, i.e. galenical aspects of topical compositions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps

Abstract

The invention relates to a traditional Chinese medicine fumigation and washing bag soaking agent for treating knee osteoarthritis and a preparation method thereof. The traditional Chinese medicine fumigation and washing bag soaking agent for treating knee osteoarthritis is prepared from, by weight, 65-75 parts of a juice suction medicinal material and 25-35 parts of a decoction liquid medicinal material. The juice suction medicinal material is prepared from, by weight 10-20 parts of safflower carthamus, 6-15 parts of radix angelicae, 4-8 parts of ligusticum wallichii, 6-15 parts of radix clematidis and 6-15 parts of pepper; the decoction liquid medicinal material is prepared from, by weight, 10-20 parts of erythrina indica lam, 10-20 parts of teasel roots, 10-20 parts of rhizoma drynariae, 10-20 parts of garden balsam stems, 10-20 parts of lycopodium clavatum, 10-20 parts of mistletoe, 10-20 parts of moxa, 6-15 parts of radix aconiti preparata and 6-15 parts of processed radix aconiti kusnezoffii. The bag soaking agent is remarkable in clinical curative effect on treatment of osteoarthritis, particularly knee osteoarthritis and has the effects of nourishing the liver and the kidney, strengthening tendons and bones, promoting qi to activate blood, dispelling cold and removing dampness, activating meridians to stop pain and the like.

Description

A kind of Chinese medicine fumigation and washing bagged steeping drug for the treatment of knee osteoarthritis and preparation method thereof
Technical field
The present invention relates to a kind of Chinese medicine fumigation and washing bagged steeping drug for the treatment of knee osteoarthritis and preparation method thereof.
Background technology
Knee osteoarthritis (kneeosteoarthritis, KOA) is a kind of chronic degenerative joint disease, and be its feature with the gonalgia, swelling, stiff and dysfunction clinically, be more common in middle-aged and elderly people, women is more than male.Chinese medicine is thought, knee osteoarthritis belongs to the traditional Chinese medical science " rheumatism involving the bone " category, is that long-term strain, stagnation of QI and blood, muscles and bones cervical carcinoma with cachexia, the aspect combined influences such as wind-cold damp pathogen invasion and attack, blockage of main and collateral channels, the resistance of joint numbness form because worn with age, caused by liver and kidney deficiency, muscles and bones mistake are supported.Its basic pathogenesis is " deficient, the wind and cold of Liver and kidney QI and blood wet arthralgia due to blood stasis resistance ", and therefore the Therapeutic Principle of primary disease is based on invigorating the liver and kidney, hold concurrently wind-damp dispelling, dispersing cold for relieving pain, easing the affected liver relaxing muscles and tendons etc.
Chinese medicine fumigation and washing therapy is the large feature therapies of one in external treatment of Chinese medicine, be utilize Chinese medicine decoction to carry out fumigating, the Therapeutic Method in dipping bath affected part.Chinese medicine fumigation and washing therapy will have expelling wind and cold by heating power, the medicine of channels sootheing and network vessel quickening analgesic effect directly acts on suffers from knee joint local, infiltration skin, through sick institute, improve local blood circulation, thus reduce intra-osteal pressure, promote that inflammation absorbs, alleviate or eliminate symptom, there is the double treatment effect of physical therapy and Chinese medicine; Avoid medicine to the infringement of gastrointestinal tract and liver etc. simultaneously, also avoid gastrointestinal tract and liver on the impact of medicine, thus improve the availability of medicine.But still there is the shortcoming that curative effect is good not, use inconvenience in the Chinese medicine fuming-lotion of current existing treatment knee osteoarthritis, and the fat-soluble effective ingredient that there is medical material due to Chinese medicine fumigation and washing therapy is difficult to stripping, decoction process is loaded down with trivial details, not easily preserve, the problem such as not portable, has had a strong impact on applying of the method.
Summary of the invention
The object of the present invention is to provide a kind of medicine heat with helping, rapid-action, stripping rapidly and Chinese medicine fumigation and washing bagged steeping drug of the high treatment knee osteoarthritis of leaching rate and preparation method thereof.
Object of the present invention is achieved through the following technical solutions: a kind of Chinese medicine fumigation and washing bagged steeping drug for the treatment of knee osteoarthritis, and with mass ratio range, it is prepared from primarily of the suction juice medical material of 65 ~ 75 parts and the decoction liquor medical material of 25 ~ 35 parts; Described decoction liquor medical material directly mixes with suction juice medical material after decocting extraction and be concentrated.
Wherein, described suction juice medical material, with mass ratio range, comprises 10 ~ 20 parts, Flos Carthami, the Radix Angelicae Dahuricae 6 ~ 15 parts, Rhizoma Chuanxiong 4 ~ 8 parts, Radix Clematidis 6 ~ 15 parts, 6 ~ 15 parts, Pericarpium Zanthoxyli;
Described decoction liquor medical material, with mass ratio range, comprise Cortex erythrinae 10 ~ 20 parts, Radix Dipsaci 10 ~ 20 parts, Rhizoma Drynariae 10 ~ 20 parts, Herba speranskiae tuberculatae 10 ~ 20 parts, Herba Lycopodii 10 ~ 20 parts, Herba Visci 10 ~ 20 parts, Folium Artemisiae Argyi 10 ~ 20 parts, Radix Aconiti Preparata 6 ~ 15 parts, Radix Aconiti Kusnezoffii Preparata 6 ~ 15 parts.
The preparation method of the Chinese medicine fumigation and washing bagged steeping drug of described treatment knee osteoarthritis, it comprises the following steps:
(1) each component raw material is taken by weight ratio, stand-by;
(2) pulverizing of juice medical material is inhaled: be ground into fine powder by after the Flos Carthami weighed up, the Radix Angelicae Dahuricae, Rhizoma Chuanxiong, Radix Clematidis, Pericarpium Zanthoxyli mixing, fine powder crosses 20 ~ 60 object screen clothes, must inhale juice fine powder, for subsequent use;
(3) extraction of decoction liquor: be ground into coarse powder by after the Cortex erythrinae weighed up, Radix Dipsaci, Rhizoma Drynariae, Herba speranskiae tuberculatae, Herba Lycopodii, Herba Visci, Folium Artemisiae Argyi, Radix Aconiti Preparata, Radix Aconiti Kusnezoffii Preparata mixing, then in coarse powder, water soaking 10 ~ 30min is added, wherein the addition of water is 12 ~ 20 times of gross weight before raw material soaking for the preparation of decoction liquor medical material, decoct extraction 1 ~ 5 time afterwards, each decocting time is 0.5 ~ 1.5h, merge each decoction liquor, decoction liquor after merging is filtered, obtains filtrate A;
(4) concentrated: the filtrate A of step (3) gained being carried out evaporation and concentration one-tenth is the thick paste of 1.15 ~ 1.37 relative to the relative density of water, for subsequent use;
(5) mix: the suction juice fine powder that step (4) gained thick paste and step (2) obtain is carried out Homogeneous phase mixing, suction juice fine powder is adsorbed thick paste, after thick paste adsorbs completely, there is the suction juice fine powder of thick paste to carry out drying suction, obtain dried powder; Adopt suction juice medical material to adsorb thick paste in this step, according to the amount of thick paste, primary sorption is not complete, adsorbs, until adsorbed completely by thick paste after can the suction juice fine powder of thick paste being had to dry suction again.
(6) preparation of granule: in the dried powder that step (5) obtains, add the ethanol that concentration is 55% ~ 60%, carry out wet granulation, obtained granule is crossed 10 ~ 12 object screen clothes, dry 7 ~ 9h in the baking oven of 40 ~ 45 DEG C, obtains drug particles finally afterwards.When to add concentration be the ethanol wet granulation of 55% ~ 60%, the addition of ethanol controls to be advisable can be convenient to wet granulation.
Wherein the ethanol of 55% ~ 60% is obtained by the ethanol thin up of concentration 90% ~ 95%;
Decoction liquor after being combined in step (3) is filtered, and the filter method adopted filters for utilizing 4 layers of gauze, and described gauze is 15 ~ 20 orders.
The concrete grammar of the evaporation and concentration in step (4) is: first the filtrate A of step (3) gained is carried out rotary evaporation, the temperature of evaporation is 45 ~ 55 DEG C, the time of evaporation is 6 ~ 8h, then water-bath concentrates, the bath temperature that water-bath concentrates is 85 ~ 95 DEG C, and water bath time is 5 ~ 7h.
Have the suction juice fine powder of thick paste to carry out drying suction in step (5), the drying means adopted is vacuum drying 23 ~ 25h, and baking temperature is temperature 55 ~ 65 DEG C, and vacuum is-0.09 ~-0.08Mpa.
The water content of the drug particles obtained after drying in step (6) is lower than 8%.
The preparation method of the Chinese medicine fumigation and washing bagged steeping drug of described treatment knee osteoarthritis, it also comprises step (7), described step (7) is loaded in non-woven fabrics by the drug particles of step (6) gained, uses aluminium foil paper bag packing afterwards.
The present invention is to the screening of inhaling juice pulverizing medicinal materials granularity, and according to diffusion law, the amount of diffusate and the particle radii of diffusate are inversely proportional to, and granule is less, leaches amount of substance more.Granular size affects the leaching rate of bagged steeping drug and the key factor of leaching rate, after decoction pieces is pulverized, has larger diffusion area, strengthens water to the infiltration of medical material, dissolving, diffusion.Leaching process is that solvent enters cell, and material in continuous dissolved cell, the concentration of intracellular matter constantly increases, and make intraor extracellular occur obvious concentration difference, then intracellular solutes spreads to extracellular.Meanwhile, along with increasing of degree of grinding, the pulverized chance of cell increases, tolerant directly the contact with water of cell and dissolving, and stripping quantity is increased.After deliberation, the grinding particle size of bagged steeping drug can be controlled in 10 ~ 60 orders, and different products can select optimal granularity scope according to its character etc.Feature the present invention according to prescription of the present invention controls grinding particle size within the scope of 20 ~ 60 orders.
Bagged steeping drug prepared by this technique:
1 appearance character: granule is dry, uniform particles, color and luster is consistent, without the moisture absorption, softens, lumps, ridicules the phenomenons such as solution.
2 losss on drying: measure according to dry weightless mensuration, be dried to constant weight in 105 DEG C, weight loss is lower than 2.0%.
3 moisture: measure, lower than 6.0% according to aquametry.
4 granularities: do not exceed 15% of confession examination amount by a sieve (2000 μm) and the summation of sieving (180 μm) by No. five.
5 content uniformity: the content uniformity limit of the granule of unit dose package, meets the regulation of table 13.
Table 13 granule content uniformity bound requirements
Average loading amount (g) Sabot difference limit (%)
Less than 1.0 and 1.0 ±10.0
More than 1.0 to 1.5 ±8.0
More than 1.5 to 6.0 ±7.0
6.0 more than ±5.0
Compared to prior art, the invention has the advantages that: (1) bagged steeping drug formula of the present invention shows through clinical research test, treatment clinical efficacy for osteoarthritis especially knee osteoarthritis is remarkable, there is invigorating the liver and kidney, strengthening the tendons and bones, effect of promoting flow of QI and blood, dispelling cold and removing dampness, removing obstruction in the collateral to relieve pain, support for worn with age, Liver and kidney deficiency of qi and blood, muscles and bones mistake, long-term strain, muscles and bones are impaired, stagnation of QI and blood, wind-cold damp pathogen invasion and attack, meridians joint numbness hinder the aspects such as obstructed, real is treatment caused by liver and kidney deficiency, the good recipe of the arthromyodynia of anemofrigid-damp arthralgia resistance.
(2) the present invention is after pharmacodynamic experiment, and it is remarkable that observation index shows this bagged steeping drug anti-inflammatory and analgesic effect.There is instant effect, curative effect be high, cure rate is high, the feature such as few side effects, can life-time service for arthroxerosis disease, the knee osteoarthritis Chinese medicine fumigation and washing bagged steeping drug that preparation method of the present invention is produced is easy to use, the therapeutic equivalence of its curative effect and traditional water decoction fumigation and wash method.The present invention is raw materials used, is pure Chinese medicinal preparation, does not find erythra, irritated grade for untoward reaction, safety is high.
(3) carry out water extraction after adopting the prepared slices of Chinese crude drugs to be ground into coarse powder in extract drugs process of the present invention, saved medical material, make that effective ingredient is more more easily to be extracted, improve the extraction rate of effective ingredient.
(4) bagged steeping drug is the one improvement dosage form of Chinese medicine traditional decoction, both the advantage that decoction is rapid-action had been maintained, turn avoid the long-time trouble decocted, and preparation technology is simple, easy to use, be easy to carry and preservation, only need soak with boiled water during use, be easy to be accepted by extensive patients, be a kind of rising dosage form.
(5) bagged steeping drug except keeping decoction to be originally convenient to absorb, the feature such as rapid-action, also have consumption standard, stripping is rapid, leaching rate is high, carry and the advantage such as easy to use, in innovation of Chinese medicinal dosage form, development prospect is good greatly.Present invention process technical quality is controlled well, and repeatable strong, with low cost, raw material easily obtains, and is easy to promote the use of.
Accompanying drawing explanation
Fig. 1 is the process chart of the embodiment of the present invention eight.
Fig. 2 is that the bagged steeping drug that obtains of the present invention affects bar diagram to capillary permeability.
Detailed description of the invention
Below in conjunction with embodiment, content of the present invention is described in detail:
Embodiment one:
The preparation method of the Chinese medicine fumigation and washing bagged steeping drug for the treatment of knee osteoarthritis, it comprises the following steps:
(1) each component raw material is taken by weight ratio, stand-by;
(2) pulverizing of juice medical material is inhaled: be ground into fine powder by after 10 parts, the Flos Carthami weighed up, the Radix Angelicae Dahuricae 15 parts, Rhizoma Chuanxiong 4 parts, Radix Clematidis 15 parts, the mixing of 6 parts, Pericarpium Zanthoxyli, fine powder crosses 60 object screen clothes, must inhale juice fine powder, for subsequent use;
(3) extraction of decoction liquor: by the Cortex erythrinae 10 parts weighed up, Radix Dipsaci 20 parts, Rhizoma Drynariae 10 parts, Herba speranskiae tuberculatae 20 parts, Herba Lycopodii 10 parts, Herba Visci 20 parts, Folium Artemisiae Argyi 10 parts, Radix Aconiti Preparata 15 parts, coarse powder is ground into after Radix Aconiti Kusnezoffii Preparata 6 parts mixing, then in coarse powder, water soaking 30min is added, wherein the addition of water is: for the preparation of 12 times of gross weight before the raw material soaking of decoction liquor medical material, decoct extraction 1 time afterwards, each decocting time is 0.5h, merge each decoction liquor, decoction liquor after merging is filtered, wherein, the filter method adopted filters for utilizing 4 layers of gauze, described gauze is 15 orders, obtain filtrate A,
(4) concentrated: the filtrate A of step (3) gained being carried out evaporation and concentration one-tenth is the thick paste of 1.15 relative to the relative density of water, for subsequent use; Wherein, the concrete grammar of evaporation and concentration is: first the filtrate A of step (3) gained is carried out rotary evaporation, and the temperature of evaporation is 45 DEG C, and the time of evaporation is 8h, and then water-bath concentrates, and the bath temperature that water-bath concentrates is 85 DEG C, and water bath time is 7h.
(5) mix: the suction juice fine powder that step (4) gained thick paste and step (2) obtain is carried out Homogeneous phase mixing, suction juice fine powder is adsorbed thick paste, after thick paste adsorbs completely, there is the suction juice fine powder of thick paste to carry out drying suction, obtain dried powder; Wherein, the drying means adopted is vacuum drying 23h, and baking temperature is temperature 65 DEG C, and vacuum is-0.08Mpa.
(6) preparation of granule: in the dried powder that step (5) obtains, add the ethanol that concentration is 55%, carry out wet granulation, obtained granule is crossed 10 object screen clothes, dry 7h in the baking oven of 45 DEG C afterwards, obtain drug particles finally, the water content of the drug particles obtained after drying is lower than 8%.
(7) drug particles of step (6) gained is loaded in non-woven fabrics, use aluminium foil paper bag packing afterwards.
Embodiment two:
The preparation method of the Chinese medicine fumigation and washing bagged steeping drug for the treatment of knee osteoarthritis, it comprises the following steps:
(1) each component raw material is taken by weight ratio, stand-by;
(2) pulverizing of juice medical material is inhaled: be ground into fine powder by after 15 parts, the Flos Carthami weighed up, the Radix Angelicae Dahuricae 10 parts, Rhizoma Chuanxiong 5 parts, Radix Clematidis 10 parts, the mixing of 8 parts, Pericarpium Zanthoxyli, fine powder crosses 30 object screen clothes, must inhale juice fine powder, for subsequent use;
(3) extraction of decoction liquor: by the Cortex erythrinae 15 parts weighed up, Radix Dipsaci 15 parts, Rhizoma Drynariae 15 parts, Herba speranskiae tuberculatae 15 parts, Herba Lycopodii 15 parts, Herba Visci 15 parts, Folium Artemisiae Argyi 15 parts, Radix Aconiti Preparata 10 parts, coarse powder is ground into after Radix Aconiti Kusnezoffii Preparata 10 parts mixing, then in coarse powder, water soaking 20min is added, wherein the addition of water is: for the preparation of 12 times of gross weight before the raw material soaking of decoction liquor medical material, decoct extraction 2 times afterwards, each decocting time is 1h, merge each decoction liquor, decoction liquor after merging is filtered, wherein, the filter method adopted filters for utilizing 4 layers of gauze, described gauze is 20 orders, obtain filtrate A,
(4) concentrated: the filtrate A of step (3) gained being carried out evaporation and concentration one-tenth is the thick paste of 1.35 relative to the relative density of water, for subsequent use; Wherein, the concrete grammar of evaporation and concentration is: first the filtrate A of step (3) gained is carried out rotary evaporation, and the temperature of evaporation is 50 DEG C, and the time of evaporation is 7h, and then water-bath concentrates, and the bath temperature that water-bath concentrates is 90 DEG C, and water bath time is 6h.
(5) mix: the suction juice fine powder that step (4) gained thick paste and step (2) obtain is carried out Homogeneous phase mixing, suction juice fine powder is adsorbed thick paste, after thick paste adsorbs completely, there is the suction juice fine powder of thick paste to carry out drying suction, obtain dried powder; Wherein, the drying means adopted is vacuum drying 24h, and baking temperature is temperature 60 C, and vacuum is-0.09Mpa.
(6) preparation of granule: in the dried powder that step (5) obtains, add the ethanol that concentration is 56%, carry out wet granulation, obtained granule is crossed 12 object screen clothes, dry 8h in the baking oven of 45 DEG C afterwards, obtain drug particles finally, the water content of the drug particles obtained after drying is lower than 8%.
(7) drug particles of step (6) gained is loaded in non-woven fabrics, use aluminium foil paper bag packing afterwards.
Embodiment three:
The preparation method of the Chinese medicine fumigation and washing bagged steeping drug for the treatment of knee osteoarthritis, it comprises the following steps:
(1) each component raw material is taken by weight ratio, stand-by;
(2) pulverizing of juice medical material is inhaled: be ground into fine powder by after 18 parts, the Flos Carthami weighed up, the Radix Angelicae Dahuricae 12 parts, Rhizoma Chuanxiong 6 parts, Radix Clematidis 12 parts, the mixing of 12 parts, Pericarpium Zanthoxyli, fine powder crosses 50 object screen clothes, must inhale juice fine powder, for subsequent use;
(3) extraction of decoction liquor: by the Cortex erythrinae 18 parts weighed up, Radix Dipsaci 18 parts, Rhizoma Drynariae 18 parts, Herba speranskiae tuberculatae 18 parts, Herba Lycopodii 18 parts, Herba Visci 18 parts, Folium Artemisiae Argyi 18 parts, Radix Aconiti Preparata 12 parts, coarse powder is ground into after Radix Aconiti Kusnezoffii Preparata 12 parts mixing, then in coarse powder, water soaking 15min is added, wherein the addition of water is: for the preparation of 15 times of gross weight before the raw material soaking of decoction liquor medical material, decoct extraction 3 times afterwards, each decocting time is 1.5h, merge each decoction liquor, decoction liquor after merging is filtered, wherein, the filter method adopted filters for utilizing 4 layers of gauze, described gauze is 18 orders, obtain filtrate A,
(4) concentrated: the filtrate A of step (3) gained being carried out evaporation and concentration one-tenth is the thick paste of 1.25 relative to the relative density of water, for subsequent use; Wherein, the concrete grammar of evaporation and concentration is: first the filtrate A of step (3) gained is carried out rotary evaporation, and the temperature of evaporation is 48 DEG C, and the time of evaporation is 6.5h, and then water-bath concentrates, and the bath temperature that water-bath concentrates is 88 DEG C, and water bath time is 5.5h.
(5) mix: the suction juice fine powder that step (4) gained thick paste and step (2) obtain is carried out Homogeneous phase mixing, suction juice fine powder is adsorbed thick paste, after thick paste adsorbs completely, there is the suction juice fine powder of thick paste to carry out drying suction, obtain dried powder; Wherein, the drying means adopted is vacuum drying 23.5h, and baking temperature is temperature 58 DEG C, and vacuum is-0.085Mpa.
(6) preparation of granule: in the dried powder that step (5) obtains, add the ethanol that concentration is 55%, carry out wet granulation, obtained granule is crossed 11 object screen clothes, dry 7.5h in the baking oven of 42 DEG C afterwards, obtain drug particles finally, the water content of the drug particles obtained after drying is lower than 8%.
(7) drug particles of step (6) gained is loaded in non-woven fabrics, use aluminium foil paper bag packing afterwards.
Embodiment four:
The preparation method of the Chinese medicine fumigation and washing bagged steeping drug for the treatment of knee osteoarthritis, it comprises the following steps:
(1) each component raw material is taken by weight ratio, stand-by;
(2) pulverizing of juice medical material is inhaled: be ground into fine powder by after 20 parts, the Flos Carthami weighed up, the Radix Angelicae Dahuricae 6 parts, Rhizoma Chuanxiong 8 parts, Radix Clematidis 6 parts, the mixing of 15 parts, Pericarpium Zanthoxyli, fine powder crosses 20 object screen clothes, must inhale juice fine powder, for subsequent use;
(3) extraction of decoction liquor: by the Cortex erythrinae 20 parts weighed up, Radix Dipsaci 10 parts, Rhizoma Drynariae 20 parts, Herba speranskiae tuberculatae 10 parts, Herba Lycopodii 20 parts, Herba Visci 10 parts, Folium Artemisiae Argyi 20 parts, Radix Aconiti Preparata 6 parts, coarse powder is ground into after Radix Aconiti Kusnezoffii Preparata 15 parts mixing, then in coarse powder, water soaking 10min is added, wherein the addition of water is: for the preparation of 15 times of gross weight before the raw material soaking of decoction liquor medical material, decoct extraction 5 times afterwards, each decocting time is 0.5h, merge each decoction liquor, decoction liquor after merging is filtered, wherein, the filter method adopted filters for utilizing 4 layers of gauze, described gauze is 20 orders, obtain filtrate A,
(4) concentrated: the filtrate A of step (3) gained being carried out evaporation and concentration one-tenth is the thick paste of 1.37 relative to the relative density of water, for subsequent use; Wherein, the concrete grammar of evaporation and concentration is: first the filtrate A of step (3) gained is carried out rotary evaporation, and the temperature of evaporation is 55 DEG C, and the time of evaporation is 6h, and then water-bath concentrates, and the bath temperature that water-bath concentrates is 95 DEG C, and water bath time is 5h.
(5) mix: the suction juice fine powder that step (4) gained thick paste and step (2) obtain is carried out Homogeneous phase mixing, suction juice fine powder is adsorbed thick paste, after thick paste adsorbs completely, there is the suction juice fine powder of thick paste to carry out drying suction, obtain dried powder; Wherein, the drying means adopted is vacuum drying 25h, and baking temperature is temperature 55 DEG C, and vacuum is-0.09Mpa.
(6) preparation of granule: in the dried powder that step (5) obtains, add the ethanol wet granulation that concentration is 58%, obtained granule is crossed 12 object screen clothes, dry 9h in the baking oven of 40 DEG C afterwards, obtain drug particles finally, the water content of the drug particles obtained after drying is lower than 8%.
(7) drug particles of step (6) gained is loaded in non-woven fabrics, use aluminium foil paper bag packing afterwards.
Embodiment five:
The preparation method of the Chinese medicine fumigation and washing bagged steeping drug for the treatment of knee osteoarthritis, it comprises the following steps:
(1) each component raw material is taken by weight ratio, stand-by;
(2) pulverizing of juice medical material is inhaled: be ground into fine powder by after 10 parts, the Flos Carthami weighed up, the Radix Angelicae Dahuricae 15 parts, Rhizoma Chuanxiong 4 parts, Radix Clematidis 15 parts, the mixing of 6 parts, Pericarpium Zanthoxyli, fine powder crosses 60 object screen clothes, must inhale juice fine powder, for subsequent use;
(3) extraction of decoction liquor: by the Cortex erythrinae 10 parts weighed up, Radix Dipsaci 20 parts, Rhizoma Drynariae 10 parts, Herba speranskiae tuberculatae 20 parts, Herba Lycopodii 10 parts, Herba Visci 20 parts, Folium Artemisiae Argyi 10 parts, Radix Aconiti Preparata 15 parts, coarse powder is ground into after Radix Aconiti Kusnezoffii Preparata 6 parts mixing, then in coarse powder, water soaking 30min is added, wherein the addition of water is: for the preparation of 15 times of gross weight before the raw material soaking of decoction liquor medical material, decoct extraction 1 time afterwards, each decocting time is 1h, merge each decoction liquor, decoction liquor after merging is filtered, wherein, the filter method adopted filters for utilizing 4 layers of gauze, described gauze is 15 orders, obtain filtrate A,
(4) concentrated: the filtrate A of step (3) gained being carried out evaporation and concentration one-tenth is the thick paste of 1.15 relative to the relative density of water, for subsequent use; Wherein, the concrete grammar of evaporation and concentration is: first the filtrate A of step (3) gained is carried out rotary evaporation, and the temperature of evaporation is 45 DEG C, and the time of evaporation is 8h, and then water-bath concentrates, and the bath temperature that water-bath concentrates is 85 DEG C, and water bath time is 7h.
(5) mix: the suction juice fine powder that step (4) gained thick paste and step (2) obtain is carried out Homogeneous phase mixing, suction juice fine powder is adsorbed thick paste, after thick paste adsorbs completely, there is the suction juice fine powder of thick paste to carry out drying suction, obtain dried powder; Wherein, the drying means adopted is vacuum drying 23h, and baking temperature is temperature 65 DEG C, and vacuum is-0.085Mpa.
(6) preparation of granule: in the dried powder that step (5) obtains, add the ethanol that concentration is 60%, carry out wet granulation, obtained granule is crossed 10 object screen clothes, dry 7h in the baking oven of 45 DEG C afterwards, obtain drug particles finally, the water content of the drug particles obtained after drying is lower than 8%.
(7) drug particles of step (6) gained is loaded in non-woven fabrics, use aluminium foil paper bag packing afterwards.
Embodiment six:
The preparation method of the Chinese medicine fumigation and washing bagged steeping drug for the treatment of knee osteoarthritis, it comprises the following steps:
(1) each component raw material is taken by weight ratio, stand-by;
(2) pulverizing of juice medical material is inhaled: be ground into fine powder by after 15 parts, the Flos Carthami weighed up, the Radix Angelicae Dahuricae 10 parts, Rhizoma Chuanxiong 5 parts, Radix Clematidis 10 parts, the mixing of 8 parts, Pericarpium Zanthoxyli, fine powder crosses 30 object screen clothes, must inhale juice fine powder, for subsequent use;
(3) extraction of decoction liquor: by the Cortex erythrinae 15 parts weighed up, Radix Dipsaci 15 parts, Rhizoma Drynariae 15 parts, Herba speranskiae tuberculatae 15 parts, Herba Lycopodii 15 parts, Herba Visci 15 parts, Folium Artemisiae Argyi 15 parts, Radix Aconiti Preparata 10 parts, coarse powder is ground into after Radix Aconiti Kusnezoffii Preparata 10 parts mixing, then in coarse powder, water soaking 20min is added, wherein the addition of water is: for the preparation of 15 times of gross weight before the raw material soaking of decoction liquor medical material, decoct extraction 3 times afterwards, each decocting time is 1.5h, merge each decoction liquor, decoction liquor after merging is filtered, wherein, the filter method adopted filters for utilizing 4 layers of gauze, described gauze is 20 orders, obtain filtrate A,
(4) concentrated: the filtrate A of step (3) gained being carried out evaporation and concentration one-tenth is the thick paste of 1.35 relative to the relative density of water, for subsequent use; Wherein, the concrete grammar of evaporation and concentration is: first the filtrate A of step (3) gained is carried out rotary evaporation, and the temperature of evaporation is 50 DEG C, and the time of evaporation is 7h, and then water-bath concentrates, and the bath temperature that water-bath concentrates is 90 DEG C, and water bath time is 6h.
(5) mix: the suction juice fine powder that step (4) gained thick paste and step (2) obtain is carried out Homogeneous phase mixing, suction juice fine powder is adsorbed thick paste, after thick paste adsorbs completely, there is the suction juice fine powder of thick paste to carry out drying suction, obtain dried powder; Wherein, the drying means adopted is vacuum drying 24h, and baking temperature is temperature 60 C, and vacuum is-0.087Mpa.
(6) preparation of granule: in the dried powder that step (5) obtains, add the ethanol that concentration is 55%, carry out wet granulation, obtained granule is crossed 12 object screen clothes, dry 8h in the baking oven of 45 DEG C afterwards, obtain drug particles finally, the water content of the drug particles obtained after drying is lower than 8%.
(7) drug particles of step (6) gained is loaded in non-woven fabrics, use aluminium foil paper bag packing afterwards.
Embodiment seven:
The preparation method of the Chinese medicine fumigation and washing bagged steeping drug for the treatment of knee osteoarthritis, it comprises the following steps:
(1) each component raw material is taken by weight ratio, stand-by;
(2) pulverizing of juice medical material is inhaled: be ground into fine powder by after 18 parts, the Flos Carthami weighed up, the Radix Angelicae Dahuricae 12 parts, Rhizoma Chuanxiong 6 parts, Radix Clematidis 12 parts, the mixing of 12 parts, Pericarpium Zanthoxyli, fine powder crosses 50 object screen clothes, must inhale juice fine powder, for subsequent use;
(3) extraction of decoction liquor: by the Cortex erythrinae 18 parts weighed up, Radix Dipsaci 18 parts, Rhizoma Drynariae 18 parts, Herba speranskiae tuberculatae 18 parts, Herba Lycopodii 18 parts, Herba Visci 18 parts, Folium Artemisiae Argyi 18 parts, Radix Aconiti Preparata 12 parts, coarse powder is ground into after Radix Aconiti Kusnezoffii Preparata 12 parts mixing, then in coarse powder, water soaking 15min is added, wherein the addition of water is: for the preparation of 20 times of gross weight before the raw material soaking of decoction liquor medical material, decoct extraction 1 time afterwards, each decocting time is 0.5h, merge each decoction liquor, decoction liquor after merging is filtered, wherein, the filter method adopted filters for utilizing 4 layers of gauze, described gauze is 18 orders, obtain filtrate A,
(4) concentrated: the filtrate A of step (3) gained being carried out evaporation and concentration one-tenth is the thick paste of 1.25 relative to the relative density of water, for subsequent use; Wherein, the concrete grammar of evaporation and concentration is: first the filtrate A of step (3) gained is carried out rotary evaporation, and the temperature of evaporation is 48 DEG C, and the time of evaporation is 6.5h, and then water-bath concentrates, and the bath temperature that water-bath concentrates is 88 DEG C, and water bath time is 5.5h.
(5) mix: the suction juice fine powder that step (4) gained thick paste and step (2) obtain is carried out Homogeneous phase mixing, suction juice fine powder is adsorbed thick paste, after thick paste adsorbs completely, there is the suction juice fine powder of thick paste to carry out drying suction, obtain dried powder; Wherein, the drying means adopted is vacuum drying 23.5h, and baking temperature is temperature 58 DEG C, and vacuum is-0.085Mpa.
(6) preparation of granule: in the dried powder that step (5) obtains, add the ethanol that concentration is 58%, carry out wet granulation, obtained granule is crossed 11 object screen clothes, dry 7.5h in the baking oven of 42 DEG C afterwards, obtain drug particles finally, the water content of the drug particles obtained after drying is lower than 8%.
(7) drug particles of step (6) gained is loaded in non-woven fabrics, use aluminium foil paper bag packing afterwards.
Embodiment eight:
The preparation method of the Chinese medicine fumigation and washing bagged steeping drug for the treatment of knee osteoarthritis, it comprises the following steps:
(1) each component raw material is taken by weight ratio, stand-by;
(2) pulverizing of juice medical material is inhaled: be ground into fine powder by after 15 parts, the Flos Carthami weighed up, the Radix Angelicae Dahuricae 10 parts, Rhizoma Chuanxiong 5 parts, Radix Clematidis 10 parts, the mixing of 8 parts, Pericarpium Zanthoxyli, fine powder crosses 30 object screen clothes, must inhale juice fine powder, for subsequent use;
(3) extraction of decoction liquor: by the Cortex erythrinae 15 parts weighed up, Radix Dipsaci 15 parts, Rhizoma Drynariae 15 parts, Herba speranskiae tuberculatae 15 parts, Herba Lycopodii 15 parts, Herba Visci 15 parts, Folium Artemisiae Argyi 15 parts, Radix Aconiti Preparata 10 parts, coarse powder is ground into after Radix Aconiti Kusnezoffii Preparata 10 parts mixing, then in coarse powder, water soaking 20min is added, wherein the addition of water is: for the preparation of 20 times of gross weight before the raw material soaking of decoction liquor medical material, decoct extraction 3 times afterwards, each decocting time is 1h, merge each decoction liquor, decoction liquor after merging is filtered, wherein, the filter method adopted filters for utilizing 4 layers of gauze, described gauze is 20 orders, obtain filtrate A,
(4) concentrated: the filtrate A of step (3) gained being carried out evaporation and concentration one-tenth is the thick paste of 1.35 relative to the relative density of water, for subsequent use; Wherein, the concrete grammar of evaporation and concentration is: first the filtrate A of step (3) gained is carried out rotary evaporation, and the temperature of evaporation is 50 DEG C, and the time of evaporation is 7h, and then water-bath concentrates, and the bath temperature that water-bath concentrates is 90 DEG C, and water bath time is 6h.
(5) mix: the suction juice fine powder that step (4) gained thick paste and step (2) obtain is carried out Homogeneous phase mixing, suction juice fine powder is adsorbed thick paste, after thick paste adsorbs completely, there is the suction juice fine powder of thick paste to carry out drying suction, obtain dried powder; Wherein, the drying means adopted is vacuum drying 24h, and baking temperature is temperature 60 C, and vacuum is-0.08Mpa.
(6) preparation of granule: in the dried powder that step (5) obtains, add the ethanol that concentration is 60%, carry out wet granulation, obtained granule is crossed 12 object screen clothes, dry 8h in the baking oven of 45 DEG C afterwards, obtain drug particles finally, the water content of the drug particles obtained after drying is lower than 8%.
(7) drug particles of step (6) gained is loaded in non-woven fabrics, and outer by aluminium foil paper bag packing.
Embodiment nine:
The preparation method of the Chinese medicine fumigation and washing bagged steeping drug for the treatment of knee osteoarthritis, it comprises the following steps:
(1) each component raw material is taken by weight ratio, stand-by;
(2) pulverizing of juice medical material is inhaled: be ground into fine powder by after 18 parts, the Flos Carthami weighed up, the Radix Angelicae Dahuricae 12 parts, Rhizoma Chuanxiong 6 parts, Radix Clematidis 12 parts, the mixing of 12 parts, Pericarpium Zanthoxyli, fine powder crosses 50 object screen clothes, must inhale juice fine powder, for subsequent use;
(3) extraction of decoction liquor: by the Cortex erythrinae 18 parts weighed up, Radix Dipsaci 18 parts, Rhizoma Drynariae 18 parts, Herba speranskiae tuberculatae 18 parts, Herba Lycopodii 18 parts, Herba Visci 18 parts, Folium Artemisiae Argyi 18 parts, Radix Aconiti Preparata 12 parts, coarse powder is ground into after Radix Aconiti Kusnezoffii Preparata 12 parts mixing, then in coarse powder, water soaking 15min is added, wherein the addition of water is: for the preparation of 20 times of gross weight before the raw material soaking of decoction liquor medical material, decoct extraction 5 times afterwards, each decocting time is 1.5h, merge each decoction liquor, decoction liquor after merging is filtered, wherein, the filter method adopted filters for utilizing 4 layers of gauze, described gauze is 18 orders, obtain filtrate A,
(4) concentrated: the filtrate A of step (3) gained being carried out evaporation and concentration one-tenth is the thick paste of 1.25 relative to the relative density of water, for subsequent use; Wherein, the concrete grammar of evaporation and concentration is: first the filtrate A of step (3) gained is carried out rotary evaporation, and the temperature of evaporation is 48 DEG C, and the time of evaporation is 6.5h, and then water-bath concentrates, and the bath temperature that water-bath concentrates is 88 DEG C, and water bath time is 5.5h.
(5) mix: the suction juice fine powder that step (4) gained thick paste and step (2) obtain is carried out Homogeneous phase mixing, suction juice fine powder is adsorbed thick paste, after thick paste adsorbs completely, there is the suction juice fine powder of thick paste to carry out drying suction, obtain dried powder; Wherein, the drying means adopted is vacuum drying 23.5h, and baking temperature is temperature 58 DEG C, and vacuum is-0.09Mpa.
(6) preparation of granule: in the dried powder that step (5) obtains, add the ethanol that concentration is 55%, carry out wet granulation, obtained granule is crossed 11 object screen clothes, dry 7.5h in the baking oven of 42 DEG C afterwards, obtain drug particles finally, the water content of the drug particles obtained after drying is lower than 8%.
(7) drug particles of step (6) gained is loaded in non-woven fabrics, use aluminium foil paper bag packing afterwards.
Embodiment ten: the extraction of decoction liquor and concentrated research
Take water as solvent, decoction is carried out to decoction liquor medical material and extracts, simmer down to thick paste, with the active ingredient naringin in monarch drug Rhizoma Drynariae for index, by L9 (34) orthogonal test, bagged steeping drug extraction process is optimized, for the new drug development of said preparation lays the foundation.Specific experiment is as follows:
10.1 Orthogonal Experiment and Design
Be solvent with water, water extraction is carried out to decoction liquor medical material.With amount of water (A), decocting time (B), decoct number of times (C) 3 factors for investigating object, and draft 3 levels, test according to L9 (34) orthogonal table, Orthogonal Experiment and Design is in table 1; Using the scoring of the weighted comprehensive of dry extract yield, naringin content as the evaluation index of craft screening, to inquire into the optimum extraction process of decoction liquor.Aqueous extract is merged, is concentrated into thick paste.
Using the comprehensive grading of dry extract yield, naringin content as the measurement index determining optimum extraction process, L9 (34) orthogonal test table is selected to test, in table 2; Wherein D is classified as the error row automatically generated, the extraction process that screening is best.Naringin (I) is the effective ingredient in monarch drug Rhizoma Drynariae in compound recipe, select it as one of evaluation index, weight coefficient is respectively decided to be 0.7, and as an index of the fried indirect control of effective ingredient, effective substance extract number can judge from the size of dry extract yield (II), weight coefficient is decided to be 0.3, carries out overall merit, screening optimised process.Decoction liquor medical material in accurate title compound recipe 9 parts (these 9 parts of decoction liquor medical materials not only formula is identical but also weight also identical), extract by condition shown in L9 (34) orthogonal test, filter, merge extractive liquid, rotary evaporation concentrates, and water-bath evaporation and concentration immediately becomes dry cream, the dry 12h of vacuum drying oven, baking temperature is temperature 55 ~ 65 DEG C, and vacuum is-0.09 ~-0.08Mpa, obtains dry extract for subsequent use as each test sample.Comprehensive grading=(I/Imax × 0.7+II/IImax × 0.3) × 100.
Table 1 Orthogonal Experiment and Design
10.2 index determinings
10.2.1 the mensuration extracting solution rotary evaporation of dry extract yield concentrates to be placed on and is dried in the evaporating dish of constant weight, after water bath method, puts vacuum drying oven and is dried to constant weight, and baking temperature is temperature 55 ~ 65 DEG C, and vacuum is-0.09 ~-0.08Mpa, weighs.Calculate dry extract yield, dry extract yield (%)=dry extract amount/medical material total amount × 100%.The results are shown in Table 2, variance analysis is in table 3.
The preferred orthogonal experiments of table 2 bagged steeping drug extraction process by water
The variance analysis of table 3 dry extract yield
The variance analysis of table 4 comprehensive grading
10.2.2 measuring naringin content
10.2.2.1 the preparation of reference substance solution: the accurately weighed 2.6mg of naringin reference substance, in 25ml volumetric flask, adds methanol constant volume and obtains reference substance solution to scale, its concentration is 104 μ gml -1.
10.2.2.2 the preparation of need testing solution: precision takes the dry extract of each extracting method gained of 0.3g, in 25ml volumetric flask, adds methanol constant volume to scale, close plug, ultrasonic 10min, leaves standstill 10min, and by methanol constant volume to scale.Get supernatant, with the filtering with microporous membrane of 0.45um, obtain subsequent filtrate for subsequent use as need testing solution.The negative sample of Rhizoma Drynariae is not contained, with legal system for negative sample solution in prescription ratio and technique preparation.
10.2.2.3 chromatographic condition: chromatographic column ZORBAXSB-C18 (5 μm, 4.6 × 150mm); Mobile phase acetonitrile: 0.4% acetic acid water=18.7:81.3; Determined wavelength 283nm; Flow velocity: 1.0mLmin -1, column temperature is 25 DEG C, sample size: 10 μ L.
10.2.2.4 linear relationship is investigated: precision measures reference substance solution 0.5ml respectively, 1.0ml, 1.5ml, 2.0ml, 2.5ml be placed in 5ml volumetric flask, and add methanol constant volume to scale, concentration is respectively 10.4 μ gml -1, 20.8 μ gml -1, 31.2 μ gml -1, 41.6 μ gml -1, 52 μ gml -1, measure by condition under " chromatographic condition " item, record chromatogram.With peak area (Y) for vertical coordinate, drug quality concentration (C) is abscissa, carries out linear regression, and obtaining naringin regression equation is Y=15.037C-27.58 (r=0.9988), and result shows that naringin concentration is at 10.4 μ gml -1~ 52 μ gml -1with peak area, there is good linear relationship in scope.
10.2.2.5 precision test: precision takes 52 μ gml -1naringin reference substance solution, repeats sample introduction 6 times, records peak area, and calculate naringin content, result RSD is 0.99%, shows that instrument precision is good.
10.2.2.6 replica test: get need testing solution 6 parts, respectively sample introduction, measure naringin peak area, and calculate its RSD value.Result RSD is 1.52%, and the reproducible of the method is described.
10.2.2.7 stability test: get with a need testing solution, measures naringin peak area respectively at 0,2,4,6,8,10,12h sample introduction, and calculates its RSD value.Result RSD is 1.2%, shows that need testing solution is stable in 12h.
10.2.2.8 average recovery test: get known naringin content need testing solution 6 parts, add the naringin reference substance of homogenous quantities respectively, sample is prepared by need testing solution preparation method, analyze by above-mentioned chromatographic condition sample introduction, record peak area, result shows that naringin mean sample recovery rate be 107.4%, RSD is 1.26%.
10.3 orthogonal tests extract preferred result
The content results of the naringin obtained by 9 of Orthogonal Experiment and Design kinds of extracting method is measured respectively in table 2 according to the method under " 10.2.2 measuring naringin content " item, as shown in Table 2, each factor affects the factor order of extraction effect for decocting number of times (C) > amount of water (A) > decocting time (B), table 3 shows with the results of analysis of variance of table 4, A, B and C3 kind factor, the impact of C on dry extract yield has statistical significance (P<0.01), greater significance (P<0.05) is affected simultaneously on comprehensive grading, in conjunction with the practical situation of suitability for industrialized production, determine from naringin content, it is A3B2C3 that the comprehensive grading of dry extract yield optimizes extraction process, namely the water of 20 times is added, decoct 3 times, each 1h.
Confirmatory experiment:
In order to verify the accuracy of above-mentioned conclusion, carry out 3 tests according to optimum process condition A3B2C3.As a result, dry extract yield, naringin content meansigma methods be respectively 29.71%, 4.16mgg -1the result and Orthogonal experiment results basically identical, prove that this process stabilizing is feasible, namely in embodiment eight, the extraction process of decoction liquor is best.
Embodiment 11: bagged steeping drug molding and technology of the package research
According to the best decoction liquor extraction process gained extracting solution of embodiment eight, water-bath is condensed into thick paste, and relative density is about 1.35, with suction juice medical material Homogeneous phase mixing, dry, obtains dried powder.The dried powder ethanol wet granulation of 60%, crosses 12 eye mesh screens by obtained granule, namely obtains granule after drying afterwards at 45 DEG C, loads in non-woven bag, to obtain final product.The wet granulation technology that this research adopts, the advantages such as the granule that wet method is made, through moistened surface, has granular mass good, and good looking appearance, wearability are comparatively strong, compact property is good, apply comparatively extensive in medical industry.The principle of wet granulation is that liquid in adhesive is by the moistening of medicine powder particle surface, make to produce adhesion between powder, then under liquid bridge formation with the effect of applied mechanical power, make the method for the granule of definite shape and size, final with the form consolidation of solid bridge after drying.
Consider aluminium-foil paper have mechanical property good, be easy to processing, lightweight, to be conducive to reducing freight light-proofness good, be not easily corroded, good barrier property, the plurality of advantages such as moistureproof and waterproof, so this research employing aluminium-foil paper is packed.
The mensuration of 11.1 angle of reposes:
Adopt fixed funnel method, 3 funnels are connected and is fixed on 2 centimeters on the graph paper of horizontal positioned, the careful drug particles that the present invention finally obtained is poured in uppermost funnel along hopper walls, until medicated powder cone tips below touches bottom funnel end opening.Measure the diameter of conical base, calculate angle of repose, result is а=29.6
Result shows: this granule good fluidity.
The mensuration of 11.2 hydroscopicities:
Dry weighing botle to constant weight, precise, adds the bagged steeping drug drug particles being dried to constant weight, evenly spreads out, makes the thick about 2mm of weighing botle bottom particles.Exsiccator bottom being filled NaCl supersaturated solution puts into 25 DEG C of calorstat constant temperature 24 hours, and the relative humidity now in exsiccator is 75%.The weighing botle that the bagged steeping drug drug particles that the present invention obtains is housed is put into exsiccator, and bottle cap is opened, and 25 DEG C of constant temperature preserve 48 hours, and timing in 1,2,4,8,12,24,48 hour weighs, according to formulae discovery hydroscopicity below.The results are shown in Table 5.Wherein,
Table 5 bagged steeping drug drug particles is hydroscopicity in the environment of 75% and physical appearance at relative humidity
Result display granule moisture absorption degree is less, and physical appearance still can keep graininess after moisture absorption, relatively easily stores, and reaches the object of Study on Forming.
Embodiment 12: the Pharmacodynamics experiment of bagged steeping drug
Bagged steeping drug selected in the pharmacodynamic experiment of following bagged steeping drug is the bagged steeping drug that embodiment eight obtains.
12.1 bagged steeping drug xylol cause the impact of mice auricle swelling
12.1.1 laboratory animal grouping: get mice 40, male, be divided into blank group, Vitalin Emulgel positive controls (hereinafter referred to as diclofenac group), bagged steeping drug group (bagged steeping drug that bagged steeping drug obtains for the present invention) at random, (formula of the decoction fumigation and wash method side described in the present invention is identical with the formula of bagged steeping drug of the present invention to decoct fumigation and wash method side's group, just do not make bagged steeping drug, fumigation and wash method can be carried out after needing crude drug to decoct during use, for bagged steeping drug of the present invention, use and have inconvenience more), lower same, often organize 10.
12.1.2 medication
Blank group: still as usual raise, be left intact.
Decoct fumigation and wash method side group: the decoction fumigation and wash method side Chinese medicine decocting liquid 10ml fumigation and wash method 30min giving (39+1) DEG C 4.23%.
Bagged steeping drug group: give (39+1) DEG C 4.23% bagged steeping drug 10ml (containing crude drug amount 0.4238g) fumigation and wash method 30min.Fumigation and wash method 2 times/d, continuous fumigation and wash method 3d.
Vitalin Emulgel positive controls: give Vitalin Emulgel 2.5g/kg at every turn, embrocate auris dextra, 2 times/d, continuous 3d.
12.1.3, after collection of specimens and process last administration 30min, the both sides that dimethylbenzene 0.05ml smears mouse right ear cause inflammation, and left ear is not coated with and contrasts.Cause scorching rear 0.5h, by the mice chloral hydrate anesthesia execution of 5%, cut two ears, cut the circular auricles of two ears with the card punch that diameter is 9mm, weigh immediately, calculate ear swelling degree, the antiinflammatory action of comparative drug.
The left ear weight of average mice after average mice auris dextra weight after swelling=cause is scorching-cause is scorching, experimental result is in table 6.
12.2 bagged steeping drugs are on the impact of mice capillary permeability
12.2.1 grouping and medication are with 12.1.1 and 12.1.2, and medicine-feeding part is mouse part skin.
12.2.2 collection of specimens and process:
1h after the administration of each group of mice last, tail vein injection 1% azovan blue normal saline solution 0.1ml/10g, lumbar injection 0.6% acetic acid 0.2ml/ only, puts to death mice after 30min, cuts off abdominal cavity immediately, with 5ml normal saline flushing abdominal cavity several, collect cleaning mixture, centrifugal (3000rpm) 15min, measure trap (OD value) at 721 type spectrophotometer 590nm places, and calculate the suppression ratio that mouse peritoneal oozes out, weigh permeability with suppression ratio value.Analyze the impact that medicine increases mice capillary permeability
Suppression ratio %=(blank group OD value-medication group OD value)/blank group OD value × 100%.Experimental result is in table 7 and Fig. 2.
12.3 bagged steeping drug on Carrageenan cause the impact of rat toes swelling
12.3.1 WISTAR rat 40 is got in laboratory animal grouping, male, is divided into four groups at random, is respectively blank group, bagged steeping drug group, decocts fumigation and wash method side's group, Vitalin Emulgel positive controls, often organizes 10.
12.3.2 medication
Blank group: still as usual raise, be left intact.
Decoct fumigation and wash method side's group: give (39+1) DEG C 5.86% decoction fumigation and wash method side Chinese medicine decocting liquid 50ml (containing crude drug amount 2.934g) fumigation and wash method 30min.
Bagged steeping drug group: give (39+1) DEG C 5.86% bagged steeping drug 50ml (containing crude drug amount 2.934g) fumigation and wash method 30min.Fumigation and wash method 2 times/d, continuous fumigation and wash method 3d.
Vitalin Emulgel positive controls: at every turn give Vitalin Emulgel 2.5g/kg, embrocates right foot, 2 times/d.Continuous 3d.
12.3.3 collection of specimens and process
30min after last administration, mark in right back ankle joint, measure the right back sufficient volume of each Mus 2 times with toes capacity measurer, average as each Mus cause scorching before normal foot volume, then toes subcutaneous injection 1% chondrus ocellatus Holmes 0.1ml after every Rat Right, be administered once again simultaneously, after administration, 0.5h, 1h, 2h, 4h, 6h measure Mus foot volume, observe the changing value causing scorching rear rat foot volume, and calculate swelling, represent the anti-inflammatory effect of medicine with paw swelling, experimental result is in table 8.
12.4 bagged steeping drugs are on the impact of rat granuloma tissue
12.4.1 laboratory animal grouping and medication are with 12.3.1 and 12.3.2, and medicine-feeding part is operative region.
12.4.2 collection of specimens and process:
On the right side of each group of rat back, omoplate district is by oxter iodine disinfection, after 75% ethanol takes off iodine, and each osculum cutting 1cm length.To be weighed as the sterilizing cotton balls (above adding penicillin and streptomycin mixed liquor 0.2ml) of 20mg with ophthalmology tweezers, implant subcutaneous from minimal incision *, skin suture immediately.From operation each group of mice administration the 2nd day, continuous 12d.13d puts to death each Mus, opens former otch, is taken out by cotton balls together with surrounding connective tissue, rejects fatty tissue, puts into baking oven 60 DEG C of 12h oven dry and weighs.The weight claimed is deducted cotton balls original weight amount and namely obtain granulomatous weight, finally respectively organize the difference of granuloma weight.Analyze each group of medicine to granulomatous inhibitory action experimental result is in table 9.
12.5 bagged steeping drugs are on the impact of hot plate method physics induced pain
The same 12.1.1 12.5.1 laboratory animal is divided into groups
12.5.2 experimental index is measured and process: hot plate dolorimeter switches on power, and temperature is adjusted to 55 scholar 0.5 DEG C, and mice is put into instrument successively, and mice is from being placed on hot plate to occurring licking the pain threshold of metapedes required time as this Mus.Allly lick metapedes time <5s or >30s or leaper gives it up.Measure each group of mice pain threshold, compare between parallel group, after confirming that each group of mice pain threshold has comparability, intervene, each group respectively after last administration 0.5h measure each mice pain threshold, on mice is placed in (55 scholar 0.5) DEG C hot plate, there is licking the pain threshold of time (second) as this Mus after administration of metapedes with stopwatch record mice, the threshold of pain calculates by 60 seconds more than 60 seconds persons, the threshold of pain improvement value before and after statistics administration.
The threshold of pain improve percentage rate=(after medicine the pain sensation response time-medicine before the pain sensation response time) before/medicine the pain sensation response time × 100%, experimental result is in table 10.
12.6 bagged steeping drugs cause the impact of mice chemistry pain to glacial acetic acid
The same 12.1.1 12.6.1 laboratory animal is divided into groups.
12.6.2 experimental index is measured and process
30min after each group of administration, each Mus lumbar injection 0.6% acetum 0.2ml/ are only.Observe and occur number of times and the time of writhing response after recording injection acetum in 20min.
Calculate analgesia rate=(blank group average writhing number of times-administration group average writhing number of times)/average writhing number of times × 100% of blank group of administration group, experimental result is in table 11.
Statistical procedures
Data are all analyzed with SPSS18.0 statistical software, and take statistics learn process with t inspection, non parametric tests, be defined in 0.05 level with significant difference, result is with mean ± standard deviation represent.
Table 6 bagged steeping drug xylol causes the impact of mice auricle swelling
Note: compare * p<0.01 with blank group, ap<0.05; Compare with decoction fumigation and wash method side group, diclofenac group #p>0.05, bp>0.05.
Table 6 result shows: compare with blank group, administration group obviously suppresses mice caused by dimethylbenzene xylene auricle edema (p<0.05), and difference has significant.Compare with positive controls diclofenac group, bagged steeping drug group affects difference not obvious (p>0.05) to mice auricle swelling; Compare with decoction fumigation and wash method side group, bagged steeping drug group affects difference not obvious (p>0.05) to mice auricle swelling.
Table 7 bagged steeping drug is on the impact of capillary permeability
Note: compare with blank group, * p<0.05.
Table 7, Fig. 2 result show: compare with blank group, the mice capillary permeability increase that administration group Dichlorodiphenyl Acetate causes has obvious inhibitory action, and difference has significance (* p<0.05).Compare with blank group, the mice capillary permeability increase that bagged steeping drug group Dichlorodiphenyl Acetate causes has obvious inhibitory action, difference have pole significance ( ap<0.01).
Table 8 bagged steeping drug on Carrageenan causes the impact of rat toes swelling
Note: compare with model group *p<0.01, compares with decoction fumigation and wash method side group #p>0.05.
Table 8 result shows: medication therapy groups compares with model group, and after 0.5h, administration group significantly can alleviate rat toes swelling degree.Compare with decoction fumigation and wash method side group, bagged steeping drug group and positive controls all do not have significant difference to rat toes swelling degree.
Table 9 bagged steeping drug is on the impact of rat granuloma tissue
Note: compare with model group *p<0.01; Compare with diclofenac group ap<0.01, compares with decoction fumigation and wash method side group bp>0.05.
Table 9 result shows: medication therapy groups compares with model group, and administration group significantly can suppress the growth of granuloma induced by implantation of cotton pellets, difference significance (p<0.01).Compare with positive controls diclofenac group, the suppression of bagged steeping drug group to granuloma induced by implantation of cotton pellets does not have diclofenac group obvious, and compare with decoction fumigation and wash method side group, bagged steeping drug group is to the suppression there was no significant difference (p>0.05) of granuloma induced by implantation of cotton pellets.
Table 10 bagged steeping drug is on the impact of hot plate method physics induced pain
Note: compare with blank group, * p<0.05.
Table 10 result shows: bagged steeping drug group, decoction fumigation and wash method side group, diclofenac group all can significantly improve the logical threshold value of mice upon administration.Before administration, each group mice pain threshold compares, no significant difference (F=0.459, p=0.712).After the administration of administration group last, 30min pain threshold compares with before administration, and difference has statistical significance (P<0.01).
Table 11 bagged steeping drug causes the impact of mice chemistry pain to glacial acetic acid
Note: compare with blank group, *p<0.05; Compare with clinical group, ap<o.01; Compare with diclofenac group, bp>0.05.
Table 11 result shows: administration group is compared with blank group, and administration group can reduce the number of times that acetic acid causes mouse writhing, has obvious analgesic activity.Compare with clinical group, bagged steeping drug group has remarkable analgesic activity; Compare with diclofenac group, the analgesic activity no significant difference of bagged steeping drug group.
Clinical data:
In the present invention, bagged steeping drug is during in July, 2014 to 2014 year December, treatment Patients with Knee Osteoarthritis 60 example, and obtain significant clinical efficacy, clinical report is as follows:
1. physical data:
In 60 routine patients, man 13 example, female 47 example; The oldest person 75 years old, age reckling 45 years old, 62.68 ± 6.02 years old age; The most elder of medical history 15 years, nearly 1 month of the shortest person.This group patient all once cross other drug, physical therapy, close etc. process, all accept because of DeGrain this law treatment.
2. diagnostic criteria:
With reference to the KOA diagnostic criteria that orthopaedics branch of Chinese Medical Association formulates for 2007.1. the gonalgia repeatedly in nearly January; 2. X-ray film (standing or Weight carrying position) shows that joint space narrows, Subchondral bone sclerosis and (or) cystis degeneration, joint margins hyperosteogeny formed; 3. joint fluid (at least 2 times) is limpid, sticky, WBC < 2000/ml; 4. middle-older patient (>=40 years old); 5. morning deadlock≤30min; 6. bony crepitus (sense) is had during activity.Meet 1.+2. bar or 1.+3.+5.+6. bar or 1.+4.+5.+6. bar, diagnosable is knee osteoarthritis.
3. inclusive criteria: 1. meet KOA diagnostic criteria person; 2. age >=40 years old ,≤75 years old; 3. be ready accept investigation and can partner treatment person.
4. exclusion standard: 1. merge serious disease and the psychotics such as cardiovascular and cerebrovascular vessel, liver, lung, kidney, hemopoietic system; 2. have allergy sufferers to this group medicine, can not adhere to treatment, add and subtract medicine voluntarily, affect the treatment observe and decide person.
5. Therapeutic Method: this group patient adopts bagged steeping drug of the present invention, adds boiled water 3000ml, stirs.In medicine soup, 5ml white vinegar is added before using.Suffering limb is stretched by experimenter naturally, is inclined cross wooden barrel or basin, and knee joint is placed in washes directly over tool, and distance liquid level is about 7cm (not scald patient skin for degree).First medication steam fumigates knee about 5-10 minutes.When fluid temperature drops to about 40 DEG C, advise experimenter to be placed in by towel and wash after in tool, be wound around knee joint with towel, when band be can not help hot, again towel immersed medicine juice and be again wound around knee joint, repeatedly carrying out, about 15-20 minutes.After treatment terminates, by another block clean towel, note wind sheltering.Every day 1 dose, each 1 time of morning and afternoon, each fumigation and wash method 30min 2 weeks is a course for the treatment of, treats two courses for the treatment of altogether.
6. criterion of therapeutical effect: adopt VAS scoring to assess patient pain's degree; Lequense exponent pair patient articular function status is adopted to assess.According to the efficacy assessment standard evaluation of the new Chinese medicine treatment osteoarthritis guideline of clinical investigations in " new Chinese medicine guideline of clinical investigations ".Clinic control: arthralgia disappears completely, affected joints is movable normal, and symptom integral declines >=95%; Effective: arthralgia disappears substantially, only remaining slight arthralgia, joint motion is not limited, 70%≤symptom integral decline < 95%; Effective: arthralgia is alleviated to some extent, but activity is slightly limited, 30%≤symptom integral decline < 70%; Invalid: symptom is not improved, even continue to increase the weight of, range of motion without improvement, symptom integral decline < 30%.
7. therapeutic outcome: after 60 routine patient treatments, VAS pain scores is in table 12, Lequense index all with before treatment compares, and has significant difference (P<0.05).Have no untoward reaction.Chinese medicine fumigation and washing bagged steeping drug external curing knee osteoarthritis of the present invention can alleviate patient pain, improves function of joint, is a kind of safety, effectively, is worth further genralrlization to use.
Table 12 is treated front and back final result and is compared
8. untoward reaction: nothing.
Model case:
1, Chen, man, 60 years old, nearly 2 years of two gonalgia, recent right swelling and pain in knee obviously increased the weight of, and above all needs staircase downstairs, seat dysstasia, and meet overcast and rainy symptom obviously, morning is stiff, occasionally has interlocking phenomenon.Check: inner side tenderness under kneecap: left knee joint (+), right knee joint (++); Right knee joint is micro-swollen, right Range of motion of knee joint: 45 °; Tongue is greasy in vain, stringy and rolling pulse.Diagnosis: knee osteoarthritis.The bagged steeping drug that the fumigation and wash method embodiment of the present invention one is obtained, treat 2 courses for the treatment of, two swelling and pain in knee obviously alleviate, and all the other symptoms are also in alleviation trend, and continue 5 courses for the treatment of of fumigation and wash method, recovery from illness, follows up a case by regular visits to after half a year without recurring.
2, Liu, female, 45 years old, existing 8 years of two knee joint sorenesses, had Knee Trauma history and history of catching cold.Though through treatments such as Chinese medicine and western medicine pain relievings, two knee joint sorenesses still time stop when doing, recently, knee joint soreness increases the weight of, and stair activity all needs handrail, and seat erects difficulty.X takes the photograph sheet prompting: knee joint is that bone is loose, and joint space narrows.Check: tenderness (+) around two knee joints, mild swelling and limping, two knee articulation degree are limited to 45 °, have interlocking to levy, McMurray's test (+), lateroflexion (±), floating kneecap (±).Diagnosis: knee osteoarthritis.The bagged steeping drug that the fumigation and wash method embodiment of the present invention four is obtained, every day 1 dose, after 2 courses for the treatment of, rear two knee joints are ached and are alleviated, and swelling disappears, and all the other symptoms alleviate.Continue 4 courses for the treatment of of fumigation and wash method, recovery from illness, follow up a case by regular visits to after 1 year without recurrence.

Claims (9)

1. treat a Chinese medicine fumigation and washing bagged steeping drug for knee osteoarthritis, it is characterized in that: with mass ratio range, it is prepared from primarily of the suction juice medical material of 65 ~ 75 parts and the decoction liquor medical material of 25 ~ 35 parts;
Wherein, described suction juice medical material, with mass ratio range, comprises 10 ~ 20 parts, Flos Carthami, the Radix Angelicae Dahuricae 6 ~ 15 parts, Rhizoma Chuanxiong 4 ~ 8 parts, Radix Clematidis 6 ~ 15 parts, 6 ~ 15 parts, Pericarpium Zanthoxyli;
Described decoction liquor medical material, with mass ratio range, comprise Cortex erythrinae 10 ~ 20 parts, Radix Dipsaci 10 ~ 20 parts, Rhizoma Drynariae 10 ~ 20 parts, Herba speranskiae tuberculatae 10 ~ 20 parts, Herba Lycopodii 10 ~ 20 parts, Herba Visci 10 ~ 20 parts, Folium Artemisiae Argyi 10 ~ 20 parts, Radix Aconiti Preparata 6 ~ 15 parts, Radix Aconiti Kusnezoffii Preparata 6 ~ 15 parts.
2. the Chinese medicine fumigation and washing bagged steeping drug for the treatment of knee osteoarthritis according to claim 1, is characterized in that: described suction juice medical material, with mass ratio range, comprises 15 parts, Flos Carthami, the Radix Angelicae Dahuricae 10 parts, Rhizoma Chuanxiong 5 parts, Radix Clematidis 10 parts, 8 parts, Pericarpium Zanthoxyli.
3. the Chinese medicine fumigation and washing bagged steeping drug for the treatment of knee osteoarthritis according to claim 1, it is characterized in that: described decoction liquor medical material, with mass ratio range, comprise Cortex erythrinae 15 parts, Radix Dipsaci 15 parts, Rhizoma Drynariae 15 parts, Herba speranskiae tuberculatae 15 parts, Herba Lycopodii 15 parts, Herba Visci 15 parts, Folium Artemisiae Argyi 15 parts, Radix Aconiti Preparata 10 parts, Radix Aconiti Kusnezoffii Preparata 10 parts.
4. the preparation method of the Chinese medicine fumigation and washing bagged steeping drug of the treatment knee osteoarthritis according to claims 1 to 3 any one, is characterized in that: it comprises the following steps:
(1) each component raw material is taken by weight ratio, stand-by;
(2) pulverizing of juice medical material is inhaled: be ground into fine powder by after the Flos Carthami weighed up, the Radix Angelicae Dahuricae, Rhizoma Chuanxiong, Radix Clematidis, Pericarpium Zanthoxyli mixing, fine powder crosses 20 ~ 60 object screen clothes, must inhale juice fine powder, for subsequent use;
(3) extraction of decoction liquor: be ground into coarse powder by after the Cortex erythrinae weighed up, Radix Dipsaci, Rhizoma Drynariae, Herba speranskiae tuberculatae, Herba Lycopodii, Herba Visci, Folium Artemisiae Argyi, Radix Aconiti Preparata, Radix Aconiti Kusnezoffii Preparata mixing, then in coarse powder, water soaking 10 ~ 30min is added, wherein the addition of water is: for the preparation of 12 ~ 20 times of gross weight before the raw material soaking of decoction liquor medical material, decoct extraction 1 ~ 5 time afterwards, each decocting time is 0.5 ~ 1.5h, merge each decoction liquor, decoction liquor after merging is filtered, obtains filtrate A;
(4) concentrated: the filtrate A of step (3) gained being carried out evaporation and concentration one-tenth is the thick paste of 1.15 ~ 1.37 relative to the relative density of water, for subsequent use;
(5) mix: the suction juice fine powder that step (4) gained thick paste and step (2) obtain is carried out Homogeneous phase mixing, suction juice fine powder is adsorbed thick paste, after thick paste adsorbs completely, there is the suction juice fine powder of thick paste to carry out drying suction, obtain dried powder;
(6) preparation of granule: in the dried powder that step (5) obtains, add the ethanol that concentration is 55% ~ 60%, carry out wet granulation, obtained granule is crossed 10 ~ 12 object screen clothes, dry 7 ~ 9h in the baking oven of 40 ~ 45 DEG C, obtains drug particles finally afterwards.
5. the preparation method of the Chinese medicine fumigation and washing bagged steeping drug for the treatment of knee osteoarthritis according to claim 4, it is characterized in that: the decoction liquor after being combined in step (3) is filtered, the filter method adopted filters for utilizing 4 layers of gauze, and described gauze is 15 ~ 20 orders.
6. the preparation method of the Chinese medicine fumigation and washing bagged steeping drug for the treatment of knee osteoarthritis according to claim 4, it is characterized in that: the concrete grammar of the evaporation and concentration in step (4) is: first the filtrate A of step (3) gained is carried out rotary evaporation, the temperature of evaporation is 45 ~ 55 DEG C, the time of evaporation is 6 ~ 8h, then water-bath concentrates, the bath temperature that water-bath concentrates is 85 ~ 95 DEG C, and water bath time is 5 ~ 7h.
7. the preparation method of the Chinese medicine fumigation and washing bagged steeping drug for the treatment of knee osteoarthritis according to claim 4, it is characterized in that: in step (5), have the suction juice fine powder of thick paste to carry out drying suction, the drying means adopted is vacuum drying 23 ~ 25h, baking temperature is temperature 55 ~ 65 DEG C, and vacuum is-0.09 ~-0.08Mpa.
8. the preparation method of the Chinese medicine fumigation and washing bagged steeping drug for the treatment of knee osteoarthritis according to claim 4, is characterized in that: the water content of the drug particles obtained after drying in step (6) is lower than 8%.
9. the preparation method of the Chinese medicine fumigation and washing bagged steeping drug for the treatment of knee osteoarthritis according to claim 4, it is characterized in that: it also comprises step (7), described step (7) is loaded in non-woven fabrics by the drug particles of step (6) gained, uses aluminium foil paper bag packing afterwards.
CN201511028162.7A 2015-12-31 2015-12-31 Traditional Chinese medicine fumigation and washing bag soaking agent for treating knee osteoarthritis and preparation method thereof Pending CN105412318A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201511028162.7A CN105412318A (en) 2015-12-31 2015-12-31 Traditional Chinese medicine fumigation and washing bag soaking agent for treating knee osteoarthritis and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201511028162.7A CN105412318A (en) 2015-12-31 2015-12-31 Traditional Chinese medicine fumigation and washing bag soaking agent for treating knee osteoarthritis and preparation method thereof

Publications (1)

Publication Number Publication Date
CN105412318A true CN105412318A (en) 2016-03-23

Family

ID=55491167

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201511028162.7A Pending CN105412318A (en) 2015-12-31 2015-12-31 Traditional Chinese medicine fumigation and washing bag soaking agent for treating knee osteoarthritis and preparation method thereof

Country Status (1)

Country Link
CN (1) CN105412318A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106110227A (en) * 2016-08-31 2016-11-16 孙学成 A kind of medicine treating pain in knee osteoarthritis and preparation method thereof
CN106937901A (en) * 2017-04-28 2017-07-11 张凯 Graphene core thermal pack
CN107714887A (en) * 2017-12-01 2018-02-23 柴仪 A kind of traditional Chinese medicine lotion for treating osteoarthritis and preparation method thereof
CN111358872A (en) * 2020-04-14 2020-07-03 梁恒星 Damp medicine bath bag for muscle and tendon typhoid

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
盛录等: "膝骨痹康袋泡熏洗剂提取工艺的优化", 《风湿病与关节炎》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106110227A (en) * 2016-08-31 2016-11-16 孙学成 A kind of medicine treating pain in knee osteoarthritis and preparation method thereof
CN106937901A (en) * 2017-04-28 2017-07-11 张凯 Graphene core thermal pack
CN107714887A (en) * 2017-12-01 2018-02-23 柴仪 A kind of traditional Chinese medicine lotion for treating osteoarthritis and preparation method thereof
CN111358872A (en) * 2020-04-14 2020-07-03 梁恒星 Damp medicine bath bag for muscle and tendon typhoid

Similar Documents

Publication Publication Date Title
JP6039421B2 (en) Method for producing herbal medicine composition for anti-inflammatory / anti-tumor / pain relief and its application
CN105412318A (en) Traditional Chinese medicine fumigation and washing bag soaking agent for treating knee osteoarthritis and preparation method thereof
CN100355450C (en) Qinchuan Tongbi tablet
CN104667044A (en) Traditional Chinese medicine preparation for treating arthralgia and preparation method of traditional Chinese medicine preparation
CN101461926B (en) Medicament composition for treating gland hyperplasia and preparation method thereof
CN102100825B (en) Chinese medicine composition for treating pains and preparation method thereof
CN103405740B (en) A kind of Chinese medicine composition of external application and preparation method thereof
CN102698014B (en) Chinese medicinal composition for treating bone-related diseases and preparation method and application thereof
CN106038479B (en) A kind of frutus cnidii micro-capsule thermo-sensitive gel and preparation method thereof and quality determining method
CN102670977B (en) Chinese medicinal composition for treating arthralgia, preparation method and applications of Chinese medicinal composition
CN102657736B (en) Medicament composition for treating rheumatoid arthritis and preparation method of medicament composition
CN102302615B (en) Effective site group of daphne giraldii nitsche leaf, preparation method, medicinal composition and application thereof
CN101991811B (en) Traditional Chinese medicine composition for treating rheumatism arthralgia, cold headache, abdominal cavity pain and chilblain and preparation method thereof
CN103285330A (en) Traditional Chinese medicine for treating uterine fibroid and preparation method thereof
CN1840160A (en) Powder for resisting hyperosteogeny
CN102861159B (en) Medicine composition for treating dysmenorrheal as well as preparation method and application thereof
CN103977067A (en) Rheumatoid arthritis treatment traditional Chinese medicine composition, preparation method, detection method and application method thereof
CN104324149A (en) Medicament for dysmenorrhea and preparation method thereof
CN104096089B (en) Traditional Chinese medicine composition for treating ischemia apoplexy, and capsules and preparation method thereof
CN104814944A (en) Transdermal patch for preventing and treating lumbar disc herniation and preparation method thereof
CN103655768B (en) Chinese medicine composition for the treatment of cold-dampness type knee osteoarthritis and preparation method thereof
CN102048926A (en) Chinese medicinal pain-relieving spray for externally treating osteoarthrosis
CN104958416A (en) Chinese medicinal preparation for treating sciatica and preparation method thereof
CN102579601A (en) Medicinal composition for treating rheumatoid arthritis, and preparation method and application thereof
CN108030823A (en) A kind of Chinese medicine for treating rhinitis

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20160323