CN105409936A - 一种高浓度naa与甘油凝胶的沾根方法 - Google Patents

一种高浓度naa与甘油凝胶的沾根方法 Download PDF

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CN105409936A
CN105409936A CN201610010236.2A CN201610010236A CN105409936A CN 105409936 A CN105409936 A CN 105409936A CN 201610010236 A CN201610010236 A CN 201610010236A CN 105409936 A CN105409936 A CN 105409936A
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hormone
concentration
naa
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mother liquor
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何晴雪
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
    • A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
    • A01G9/029—Receptacles for seedlings
    • A01G9/0299—Handling or transporting of soil blocks or seedlings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Pest Control & Pesticides (AREA)
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  • Agronomy & Crop Science (AREA)
  • Forests & Forestry (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
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  • Dispersion Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Soil Sciences (AREA)
  • Botany (AREA)
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Abstract

本发明公开了一种高浓度NAA与甘油凝胶的沾根方法，属于植物生物技术领域。首先配制1mg/mL的NAA母液；将该母液稀释成500mg/L的高浓度NAA工作液；以上述工作液配制质量浓度为1%的羧甲基纤维素钠、质量浓度为8%海藻酸钠的高浓度NAA与甘油凝胶封闭液；将健康粗壮的枝条下端沾在上述凝胶封闭液中3-5秒后拿出；将上述枝条插入栽培基质中生根。

Description

一种高浓度NAA与甘油凝胶的沾根方法
技术领域
一种高浓度NAA与甘油凝胶的沾根方法，属于植物生物技术领域。
背景技术
扦插繁殖是植物、尤其是树木无性繁殖的常用方法之一，该方法具有成本低、见效快、周期短、所用枝条来源丰富且能够保持亲本优良性状等诸多优点。扦插繁殖时常常会使用到不同类型的激素，如NAA、IBA、NAA、2,4-D、GA3、6-BA、KT、TDZ、TIBA、乙烯利、B9、PP333、MH等，上述激素的添加会使植物在体或离体条件下能够提早或延迟生根、开花、坐果等，增加根数、诱导腋芽萌发、提高座果率等，在农业生产上发挥着重要的用途。然而，在离体繁殖或生根过程中难以分化的枝条由于长时间的水分或营养物质的亏损，极其容易导致死亡。而现有的采用催根诱芽开花的方法具有技术步骤较多、成本高、不稳定等缺点，在生产上效果并不理想。因此，亟需一种快速简便的方法用于植物腋芽诱导、生根及成花等。
发明内容
一种高浓度NAA与甘油凝胶的沾根方法，其特征在于，所述方法中包括有激素母液配制、工作液配制、激素凝胶配制、沾根、扦插，具体操作步骤如下：
①激素母液配制：准确称量重量为0.1g的NAA用少许95%的乙醇溶解后，用蒸馏水定容至100mL，即为1mg/mL的NAA母液；
②工作液配制：将上述母液按照体积比1:2的比率稀释成浓度为500mg/L的高浓度激素工作液，同时按照90ml激素工作液加入10ml甘油；
③激素凝胶配制：准确称取1g羧甲基纤维素钠和8g海藻酸钠，在50℃水浴条件下用100mL的浓度为500mg/L的激素与甘油工作液溶解备用；
沾根：将健康粗壮的枝条下端沾在上述凝胶封闭液中3-5秒后拿出；
扦插：将上述枝条插入栽培基质中生根。
本发明的优点在于从保水、促根、诱芽、开花、坐果等方面考虑，以激素工作液为溶剂，羧甲基纤维素钠和海藻酸钠为凝胶载体，配制成含有不同类型、浓度激素的凝胶溶液。使用时将枝条、花朵稍稍浸泡于上述溶液或者将上述溶液滴加在腋芽处，凝胶干燥后会迅速形成薄膜，达到减少水分蒸发、持续施加激素、操作简便等特点。
具体实施方式：
下面结合具体实施例对本发明进行进一步的描述。
实施例
一种高浓度NAA与甘油凝胶的沾根方法，其特征在于，所述方法中包括有激素母液配制、工作液配制、激素凝胶配制、沾根、扦插，具体操作步骤如下：
①激素母液配制：准确称量重量为0.1g的NAA用少许95%的乙醇溶解后，用蒸馏水定容至100mL，即为1mg/mL的NAA母液；
②工作液配制：将上述母液按照体积比1:2的比率稀释成浓度为500mg/L的高浓度激素工作液，在、同时按照90ml激素工作液加入10ml甘油；
③激素凝胶配制：准确称取1g羧甲基纤维素钠和8g海藻酸钠，在50℃水浴条件下用100mL的浓度为500mg/L的激素与甘油工作液溶解备用；
沾根：将8-10cm长、健康粗壮的黄杨枝条下端沾在上述凝胶封闭液中3-5秒后拿出；
扦插：将上述枝条插入栽培基质中生根。

Claims (1)

1.一种高浓度NAA与甘油凝胶的沾根方法，其特征在于，所述方法中包括有激素母液配制、工作液配制、激素凝胶配制、沾根、扦插，具体操作步骤如下：
①激素母液配制：准确称量重量为0.1g的NAA用少许95%的乙醇溶解后，用蒸馏水定容至100mL，即为1mg/mL的NAA母液；
②工作液配制：将上述母液按照体积比1:2的比率稀释成浓度为500mg/L的高浓度激素工作液，同时按照90ml激素工作液加入10ml甘油；
③激素凝胶配制：准确称取1g羧甲基纤维素钠和8g海藻酸钠，在50℃水浴条件下用100mL的浓度为500mg/L的激素与甘油工作液溶解备用；
沾根：将健康粗壮的枝条下端沾在上述凝胶封闭液中3-5秒后拿出；
扦插：将上述枝条插入栽培基质中生根。
CN201610010236.2A 2016-01-08 2016-01-08 一种高浓度naa与甘油凝胶的沾根方法 Pending CN105409936A (zh)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108812309A (zh) * 2018-05-31 2018-11-16 山西省农业科学院果树研究所 一种马铃薯脱毒原原种制备过程中扦插生根的方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008146153A2 (en) * 2007-06-01 2008-12-04 Universita' Di Pisa Method for biofertilization and bioprotection of vegetal material, in particular for in vitro propagation, protected cultivations, nurseries, and bacterial formulation for carrying out said method
CN105103884A (zh) * 2015-08-25 2015-12-02 钱夕华 一种低浓度naa与甘油凝胶的节位使用方法
CN105191919A (zh) * 2015-08-25 2015-12-30 钱夕华 一种低浓度naa与甘油凝胶的沾根方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008146153A2 (en) * 2007-06-01 2008-12-04 Universita' Di Pisa Method for biofertilization and bioprotection of vegetal material, in particular for in vitro propagation, protected cultivations, nurseries, and bacterial formulation for carrying out said method
CN105103884A (zh) * 2015-08-25 2015-12-02 钱夕华 一种低浓度naa与甘油凝胶的节位使用方法
CN105191919A (zh) * 2015-08-25 2015-12-30 钱夕华 一种低浓度naa与甘油凝胶的沾根方法

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108812309A (zh) * 2018-05-31 2018-11-16 山西省农业科学院果树研究所 一种马铃薯脱毒原原种制备过程中扦插生根的方法

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Application publication date: 20160323