CN1054029C - Process for extracting thallus protein - Google Patents
Process for extracting thallus protein Download PDFInfo
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- CN1054029C CN1054029C CN96106006A CN96106006A CN1054029C CN 1054029 C CN1054029 C CN 1054029C CN 96106006 A CN96106006 A CN 96106006A CN 96106006 A CN96106006 A CN 96106006A CN 1054029 C CN1054029 C CN 1054029C
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- ion
- tropina
- flocculation agent
- tail washings
- protein
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Abstract
The present invention relates to a process for extracting mycoprotein from high-concentration acidic organic wastewater, which comprises the steps that supersaturated air is dissolved into high-concentration organic wastewater, a flocculant is added for air flotation separation after decompression and release; the temperature of feed liquid is controlled from 30 to 80 DEG C when the flocculant is added, and the separated mycoprotein is packaged for warehousing after dewatering and drying. The present invention has the advantages of simple process, low cost, and obvious economic and social benefit; the present invention is suitable for realizing industrialized production and also has the advantages that the pollution of high-concentration acidic organic wastewater to the environment is greatly reduced, and valuable mycoprotein can be extracted from the wastewater.
Description
The present invention relates to the extraction process of tropina in the amino acid production industry, be meant especially in the amino acid fermentation extraction process, from the strongly-acid high concentration ion exchange tail washings of discharging, extract the method for corresponding tropina.
At present, the extracting method of tropina has following several:
One, fox extracting thallus protein from fermented liquid.Analyze theoretically, before extracting amino acid, at first fox extracting thallus protein helps amino acid whose extraction like this.Generally all take the disc-type supercentrifuge to divide the whizzer separating thallus abroad, it helps adopting the isoelectric point method extraction amino acid that contracts deeply.The domestic whizzer that seldom adopts removes thalline, because there is following problem in this method: (1) facility investment is too big; (2) processing power is limited; (3) bacteria-eliminating efficacy is not ideal; (4) can lose a part of fermented liquid, influence yield.
In recent years, the relevant expert of domestic monosodium glutamate industry is according to the national conditions of China, L-glutamic acid body protein in the flocculence extraction fermented liquid has been carried out number of research projects, but that uses aborning does not almost have, because study the purpose of this method is in order to improve the yield of L-glutamic acid, in fox extracting thallus protein, will inevitably cause the loss of fermentation, can reduce its yield on the contrary.
Two, from etc. fox extracting thallus protein the electric supernatant liquor, extract behind the amino acid last liquid and electric supernatant liquor such as be called, pH value etc. electric supernatant liquor is 3.0-3.2, it is relatively near the electric point for the treatment of of tropina, this is more favourable than imitating to the flocculence fox extracting thallus protein, but we find following problem by test: (1) although the degerming rate than higher (more than 90%), but fox extracting thallus protein treats still to be entrained with in the electric supernatant liquor a small amount of tropina wadding piece after this, (2) though the macromolecule organic flocculating agent major part that adds has entered in the tropina, have seldom one one and dissolve in etc. in the electric supernatant liquor; (3) treat a small amount of macromolecule organic flocculating agent that dissolves in the electric supernatant liquor and a small amount of tropina wadding piece of carrying secretly, when transferring pH value to 1.5 left and right sides, can not all divide and decompose, so when entering ion exchange column, can be adsorbed on the resin cation (R.C.).Like this, after a period of time, resin will slowly descend to amino acid whose adsorptive power, can take away a part of amino acid in the tropina that extract (4), and amino acid whose total extract yield is had certain influence.We think, it is inappropriate extracting the amino acid tropina in the electric supernatant liquor such as grade before ion-exchange, because it produces detrimentally affect to the ion-exchange meeting of back, should be placed on it after the ion-exchange.
Three, fox extracting thallus protein from the ion-exchange tail washings, the ion-exchange tail washings is that the fermented liquid during amino acid is produced freezingly waits electric supernatant liquor acid adjustment such as grade that electricity discharges to about the PH1.5 through a step, by the effluent liquid after the ion-exchange absorption amino acid, it is the strongly-acid high concentrated organic wastewater, COD is about 5000mg/l, wherein contain nearly 1% amino acid tropina (doing), also have its acid of some solubility ammonia and residual sugar etc.If with this direct discharging of waste water, not only can cause serious pollution, and be the significant wastage of resource environment.Because the pH value of ion-exchange tail washings is too low, flocculation agent general under this condition is all inapplicable, uses flocculation treatment, just will inevitably increase cost, transfers pH value if add NaOH, because Na
+Content increases, and may bring detrimentally affect to the biochemical treatment of back.If add lime Ca (OH)
2Transfer pH value,, can generate CaSO though cost is lower
4Precipitation, the tropina quality that influence is extracted.
The method of existing fox extracting thallus protein is near the tropina iso-electric point or be higher than under the pH value condition of tropina iso-electric point and carry out mostly.
The objective of the invention is to angle from environmental protection, provide a kind of under acidic conditions the new technology of fox extracting thallus protein, this technology has characteristics such as cost is low, simple to operate, effect is obvious, to overcome the defective that existing tropina extraction process exists.
For achieving the above object, the present invention has selected air supporting method water purification and flocculence to remove thalline process combined route.
At this, we are necessary to learn about earlier the characteristics of these two kinds of methods.
One, at the flocculation mechanism of strong acidic condition tropina.
(1) static characteristic of tropina:
The example that is extracted as with glu thalline protein, the pH value that is the ion-exchange tail washings is about 1.8, be significantly less than isoelectric point of protein (PH=0-5.5), at this moment glu thalline protein will exist with the cation state of complexity, and the surface that promptly is dispersed in the glu thalline protein colloidal particle in the ion-exchange tail washings will inevitably have stronger positive charge.
Because the effect of electrostatic attraction, the particle of oppositely charged is (as SO in the ion-exchange tail washings
4 2-Ion etc.), will be attracted to the Zhou Gu of tropina colloidal particle, on the interface, formed electrostatic double layer.But these negatively charged ion also are subjected to making the influence of the thermal motion that their uniform distribution open, and have the trend of leaving the micelle surface.Under the influence of these two kinds of adverse effects, electrostatic double layer just splits into two portions-adsorption layer and diffusion layer.
It mainly is the structure that has identical charges and diffusion double layer that micelle can keep the reason of dispersion state.In case, make two micelles separately, thereby stoped the gathering of micelle because the diffusion layer that pedesis makes between micelle distance narrow down to them when overlapping, promptly produces the Coulomb repulsion effect.In addition, because the hydration on micelle surface has formed and has been surrounded on micelle hydration layer on every side, also can hinder the direct gathering between micelle.
(2) cohesion mechanism:
In the ion-exchange tail washings, the tropina micelle that the surface is positively charged is owing to adsorbed electronegative on every side
4 2-Ions etc. have so just reduced the electrostatic repulsion forces between micelle.When electrostatic repulsion forces is not enough to contend with Van der Waals force between micelle, owing to the result of thermal motion causes running foul of each other of micelle to gather together.
By this cohesion mechanism, the micelle group that is gathered together remains more tiny, and is easy to broken the disintegration.The temperature height of feed liquid has a significant impact this mechanism.
(3) bridge formation flocculation mechanism:
The surface of tropina micelle or micelle has positive charge in the ion-exchange tail washings; if in the ion-exchange tail washings, add anionic organic high molecular polymer flocculation agent; owing to contain considerable active function groups (carboxyl) on its wire long-chain; have negative charge; by electrostatic attraction, the gravitational effect of pattern ylid Chuihua molecule, be adsorbed on the surface of tropina micelle or micelle consumingly between it and tropina micelle or the micelle.Many chain links of a high molecular polymer are adsorbed on respectively on the surface of different micelles or micelle, and having produced builds bridge gets and connects, and generates thick floc sedimentation, Here it is throwing out.If the mutual repulsion current potential of micelle or micelle is not too high, as long as the chain link sufficiently long of high molecular polymer, the distance of leap surpasses intergranular effective repulsion distance, also can anti-a plurality of micelles or micelle be pulled in together, cause the flocculation of building bridge.
In the selection of flocculation agent, we consider from the following aspects: the flocculating effect of flocculation agent will be got well; Flocculation agent is nontoxic; Flocculation agent should be easy to use; Ion-exchange tail washings behind the fox extracting thallus protein can not produce detrimentally affect to the processing again of back, and for this reason, we have selected a kind of linear shape polymer of reinforcing yin essence ionic organic polymer of sodium polyacrylate series to make flocculation agent.During with the tropina in its extraction ion-exchange tail washings, do not need to regulate the pH value of feed liquid, nor must add other coagulant aids, only need heat a little, and then a small amount of this flocculation agent of adding gets final product feed liquid.
Two, air supporting method mechanism
Air floating water purification technology is to manage to pass through a large amount of intensive micro bubbles in pending water, and itself and floccules are adhered to mutually, forms the buoyancy aid of whole proportion less than water, thereby relies on buoyancy to float to the water surface, to finish solid-liquid separation.
Tropina wadding piece proportion is less, is difficult for sinking, and just in time is suitable for air supporting, so we adopt the air supporting method to realize separating continuously of tropina and clear liquid.But, be difficult to reach promising result with conventional air supporting method for the ion-exchange tail washings in the L-glutamic acid production process.For this reason, we have studied the approach that realizes copolymerizing and air-float.
1, the gas characteristic of ducking in drink.Under pressure, dissolve in the pressurized air in the water, after discharging by the dissolved air release decompression, will produce a large amount of microbubbles.Bubble is more little, and the number of bubbles that forms with the air of volume is also many more, and specific surface area is big more, and microbubble and little wadding grain are by colliding adhesion, and the probability that is condensed into big flocs with gas is also many more.In addition, because bubble is little, floating speed is little, and is also little to the disturbance of water body, therefore can not crash the wadding grain.Bubble can make the local loss of vacuolar membrane when being subjected to the flow turbulence bump.May around unbalance stress, cause the imbalance of inside and outside pressure, bubble deformation breaks and and big.If in water, add the polymer substance of long-chain, then can improve the toughness and the intensity of vacuolar membrane.
2, the adhesion of bubble and wadding grain.Because the wadding grain has certain hydrophobic nature with microbubble, their specific surface area is very big again, and remaining free interface energy is arranged, and therefore, they all have mutual absorption and reduce the tendency of surface energy separately.Under certain hydraulics, have the microbubble of enough kinetic energy and wadding grain mutually during bump, squeeze each other and open the more weak outer moisture film of bonding force and close, when arranging orderly bubble internal layer moisture film and run into the residue hydrophobic group of wadding grain, adhere to by intermolecular Van der Waals force mutually.Therefore collision between the two is a soft collision.After the collision, the wadding grain realizes that with bubble multiple spot adheres to.
3, " interpolymerization " between microbubble and the little wadding grain.The ideal flocs with gas is bubble is included in wadding intragranular portion, and like this, in floating-upward process, bubble can not come off, and does not sink easily after becoming scum silica frost yet.For this reason, realize that as far as possible the little wadding grain and the collision of microbubble adhere to and continuation and big growth the in floating-upward process.This have microbubble to participate in cohesion directly and be " interpolymerization " with copolymerization of wadding grain and big process.
Comprehensive above-mentioned flocculation mechanism and air supporting mechanism, the present invention has selected a kind of approach of effective copolymerizing and air-float, fox extracting thallus protein in the ion-exchange tail washings from the L-glutamic acid production process.Its concrete scheme is that the ion-exchange tail washings is dissolved in pressurized air, adds flocculation agent again and carry out dissolved air flotation after decompression is released into, and feed temperature is controlled at 30-80 ℃ when adding flocculation agent, and isolated tropina is the packing warehouse-in after dehydration, oven dry.Be very beneficial for " interpolymerization " between bubble and the wadding grain like this, bubble and tropina wadding agllutination close closely, are difficult in the air supporting process " slag falls ", and the wadding piece is difficult for broken.
The technology of fox extracting thallus protein process following steps in the ion-exchange tail washings from the L-glutamic acid production process provided by the invention:
1, collects strongly-acid ion-exchange tail washings;
2, the strongly-acid ion-exchange tail washings of collecting is heated to 30-80 ℃;
3, molten gas feeds, dissolves pressurized air by modes such as pressurization or jets, and dissolved-air pressure is 0.15-0.35MPa;
4, discharge by the dissolved air release decompression, discharge microbubble;
5, mix, adding concentration is the sodium polyacrylate flocculation agent of 0.5-1.5 ‰, and the add-on of flocculation agent is the 1-6% of ion-exchange tail washings amount;
6, enter the gas floatation separation device protein isolate;
7, dehydration at 80-90%, after the dehydration, makes its water content reduce to 50-60% through the rapid isolated albumen water content of previous step;
8, be dried into the tropina powder, water content is packed less than 10%;
9, the clear liquid after the separation is handled the back discharging again.
In the above-mentioned steps second step also can carry out the feed liquid heating in molten gas, decompression after discharging.
The present invention can be widely used in the amino acid fermentation industry, be example only with domestic monosodium glutamate industry, the extracting method major part of L-glutamic acid all takes the freezing electricity that waits of a step to add ion-exchange second extraction technology, 1 ton of monosodium glutamate of every production, discharge the ion-exchange tail washings about 15 tons, produce 400000 tons per year by the whole nation, then will discharge 6,000,000 tons of ion-exchange tail washings every year.Use the present invention, can extract 1 ton of protein powder that contains crude protein 60-70% from 100 tons of waste water, can extract 60,000 tons of protein powders every year nearly, tropina powder price per ton is by 3000 yuan, but then every year 1.8 hundred million yuan of new output values.Every cost of tropina powder per ton adds up to about 1000 yuan, then can create 1.2 hundred million yuan of economic benefits every year.In addition, the ion-exchange tail washings behind the fox extracting thallus protein, its COD clearance is about 50%, can will alleviate half to the pollution of environment, the social benefit in institute postpartum also is very large.
In sum, the invention has the advantages that following aspect:
1, technology is simple, and is with low cost;
2, solid-liquid separation can be carried out continuously, is suitable for the realization suitability for industrialized production;
3, during fox extracting thallus protein, do not need the pH value of flavouring liquid, and only use a kind of flocculation agent, need not add other coagulant aids;
4, the tropina dehydration need not add flocculating aids, is easy to dehydration;
5, alleviate of the pollution of strongly-acid ion-exchange tail washings greatly to environment;
6, can from tail washings waste water, extract valuable tropina, the general work of economic benefit.
Embodiments of the invention are provided below.
Embodiment 1:
Present embodiment be a kind of in L-glutamic acid is produced from the ion-exchange tail washings technology of fox extracting thallus protein, this technology is to cross following steps:
1, collection of ions exchange tail washings, recording pH value is 1.8;
2, with the ion-exchange tail washings collected with being steam heated to about 45 ℃;
3, above-mentioned feed liquid feeds pressurized air by pressure air-dissolving tank in the pressure air-dissolving tank, and dissolving pressure is 0.15-0.20MPa;
4, discharge by the dissolved air release decompression, discharge little tail gas bubble;
5, adding concentration is 0.05% flocculation agent sodium polyacrylate, and add-on is 6% of an organic liquid waste amount;
6, enter the gas floatation separation device protein isolate;
7, dehydration at 80-90%, after the dehydration, makes its water content reduce to 50-60% through the rapid isolated albumen water content of previous step;
8, be dried into the tropina powder, water content is less than 10%, records to contain crude protein 65% packing;
9, the clear liquid after the separation is handled the back discharging again.
Embodiment 2:
Present embodiment still be a kind of in L-glutamic acid is produced from the ion-exchange tail washings technology of fox extracting thallus protein, this technology is through following little rapid:
1, collection of ions exchange tail washings, recording pH value is 1.6;
2, by the injecting dissolve gas device air is sucked in the above-mentioned feed liquid, dissolved-air pressure is 0.3-0.35MPa;
3, discharge by the dissolved air release decompression, discharge microbubble;
4, make feed liquid through being steam heated to about 65 ℃ by the jet heating unit;
5, by jet flow mixing device, adding concentration is 0.15% flocculation agent sodium polyacrylate, and add-on is 2% of an organic liquid waste amount;
6, enter the gas floatation separation device protein isolate;
7, dehydration at 80-90%, after the dehydration, makes its water content reduce to 50-60% through the rapid isolated albumen water content of previous step;
8, be dried into the tropina powder, water content is less than 10%, records to contain crude protein 70% the packing warehouse-in;
9, the clear liquid after the separation is handled the back discharging again.
Claims (1)
1, the technology of fox extracting thallus protein in a kind of ion-exchange tail washings from the L-glutamic acid production process, it is characterized in that through following steps: in recording the strongly-acid ion-exchange tail washings that pH value is 1.6-1.8, dissolve in pressurized air, after decompression discharges, add the flocculation agent sodium polyacrylate again and carry out dissolved air flotation, feed temperature is controlled at 30-80 ℃ when adding flocculation agent, and isolated tropina is the packing warehouse-in after dehydration, oven dry;
The flocculation agent polyacrylic acid na concn that adds in the above-mentioned processing step is 0.05-0.15%, and the add-on of this flocculation agent is the 1-6% of feed liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96106006A CN1054029C (en) | 1996-05-08 | 1996-05-08 | Process for extracting thallus protein |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96106006A CN1054029C (en) | 1996-05-08 | 1996-05-08 | Process for extracting thallus protein |
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| Publication Number | Publication Date |
|---|---|
| CN1164343A CN1164343A (en) | 1997-11-12 |
| CN1054029C true CN1054029C (en) | 2000-07-05 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN96106006A Expired - Fee Related CN1054029C (en) | 1996-05-08 | 1996-05-08 | Process for extracting thallus protein |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102517216B (en) * | 2011-12-13 | 2014-05-21 | 福建省建阳武夷味精有限公司 | Process for extracting thallus protein from concentrated glutamic acid mother liquor |
| CN111018069A (en) * | 2019-12-09 | 2020-04-17 | 河南大禹水处理有限公司 | Method for recovering microbial mycoprotein |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1060870A (en) * | 1991-10-12 | 1992-05-06 | 核工业大连应用技术研究所 | Extract the method for L-glutamic acid behind the flocculant gase floating disinfecting |
| CN1023756C (en) * | 1989-12-27 | 1994-02-16 | 福州市工业科学技术研究所 | Separation of mycoprotein in production of monosodium glutamate |
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1996
- 1996-05-08 CN CN96106006A patent/CN1054029C/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1023756C (en) * | 1989-12-27 | 1994-02-16 | 福州市工业科学技术研究所 | Separation of mycoprotein in production of monosodium glutamate |
| CN1060870A (en) * | 1991-10-12 | 1992-05-06 | 核工业大连应用技术研究所 | Extract the method for L-glutamic acid behind the flocculant gase floating disinfecting |
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| CN1164343A (en) | 1997-11-12 |
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