CN105392374A - Lipid-rich microalgal flour and method for preparing same - Google Patents
Lipid-rich microalgal flour and method for preparing same Download PDFInfo
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- CN105392374A CN105392374A CN201480040810.8A CN201480040810A CN105392374A CN 105392374 A CN105392374 A CN 105392374A CN 201480040810 A CN201480040810 A CN 201480040810A CN 105392374 A CN105392374 A CN 105392374A
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- powder
- microalgae
- air
- microalgae powder
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- 238000000034 method Methods 0.000 title claims description 32
- 150000002632 lipids Chemical class 0.000 title claims description 31
- 235000013312 flour Nutrition 0.000 title abstract 2
- 239000000843 powder Substances 0.000 claims abstract description 153
- 238000012360 testing method Methods 0.000 claims abstract description 21
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 28
- 239000007921 spray Substances 0.000 claims description 27
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims description 23
- 235000013305 food Nutrition 0.000 claims description 23
- 238000005507 spraying Methods 0.000 claims description 23
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- 238000012546 transfer Methods 0.000 description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
- GJJVAFUKOBZPCB-ZGRPYONQSA-N (r)-3,4-dihydro-2-methyl-2-(4,8,12-trimethyl-3,7,11-tridecatrienyl)-2h-1-benzopyran-6-ol Chemical class OC1=CC=C2OC(CC/C=C(C)/CC/C=C(C)/CCC=C(C)C)(C)CCC2=C1 GJJVAFUKOBZPCB-ZGRPYONQSA-N 0.000 description 1
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- 241001442242 Heterochlorella luteoviridis Species 0.000 description 1
- 229920002774 Maltodextrin Polymers 0.000 description 1
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- 241001465754 Metazoa Species 0.000 description 1
- 240000000249 Morus alba Species 0.000 description 1
- 235000008708 Morus alba Nutrition 0.000 description 1
- 241000195659 Neodesmus pupukensis Species 0.000 description 1
- 240000007817 Olea europaea Species 0.000 description 1
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- 241000297566 Saula Species 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- 238000003723 Smelting Methods 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
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- 238000010521 absorption reaction Methods 0.000 description 1
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- 229940115440 aluminum sodium silicate Drugs 0.000 description 1
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- 229940011019 arthrospira platensis Drugs 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000006701 autoxidation reaction Methods 0.000 description 1
- 239000003225 biodiesel Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
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- 239000003795 chemical substances by application Substances 0.000 description 1
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- 239000002537 cosmetic Substances 0.000 description 1
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- 238000009295 crossflow filtration Methods 0.000 description 1
- GVJHHUAWPYXKBD-UHFFFAOYSA-N d-alpha-tocopherol Natural products OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 238000005115 demineralization Methods 0.000 description 1
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- 238000007598 dipping method Methods 0.000 description 1
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- 239000001921 dulse Substances 0.000 description 1
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- 238000005984 hydrogenation reaction Methods 0.000 description 1
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- 238000009434 installation Methods 0.000 description 1
- 210000002977 intracellular fluid Anatomy 0.000 description 1
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- 230000003534 oscillatory effect Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000002161 passivation Methods 0.000 description 1
- 229910000160 potassium phosphate Inorganic materials 0.000 description 1
- 235000011009 potassium phosphates Nutrition 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
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- 238000000926 separation method Methods 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 239000000429 sodium aluminium silicate Substances 0.000 description 1
- 235000012217 sodium aluminium silicate Nutrition 0.000 description 1
- 239000002195 soluble material Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000007669 thermal treatment Methods 0.000 description 1
- 235000010384 tocopherol Nutrition 0.000 description 1
- 229930003799 tocopherol Natural products 0.000 description 1
- 239000011732 tocopherol Substances 0.000 description 1
- 229960001295 tocopherol Drugs 0.000 description 1
- 229930003802 tocotrienol Natural products 0.000 description 1
- 239000011731 tocotrienol Substances 0.000 description 1
- 229940068778 tocotrienols Drugs 0.000 description 1
- 235000019148 tocotrienols Nutrition 0.000 description 1
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- GVJHHUAWPYXKBD-IEOSBIPESA-N α-tocopherol Chemical compound OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-IEOSBIPESA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/60—Edible seaweed
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/065—Microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/02—Algae
- A61K36/05—Chlorophycota or chlorophyta (green algae), e.g. Chlorella
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Polymers & Plastics (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Biotechnology (AREA)
- Cereal-Derived Products (AREA)
- Alternative & Traditional Medicine (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention relates to microalgal flour having a particle size of between 30 and 150 [mu]m and compressibility, measured by means of POWDER TESTER HOSOKAWA, of between 45 and 55 %, characterised in that it has a flow value, determined according to a test A, of between 55 and 60 % by weight for residue at 2,000 [mu]m, dispersibility and wettability, expressed according to a test B, by the height of the product decanted in a beaker, of a value of between 0 and 2 mm; and a degree of humidification of a value of more than 70 %, and preferably of more than 80 %, of the total powder.
Description
The present invention relates to rich lipid microalgae powder, this slightly algae be Chlorella, preferably Chlorella protothecoides (Chlorellaprotothecoide).
More specifically, the present invention relates to given domain size distribution and compressed value had flow extremely significantly, the rich lipid microalgae powder of wettability and water-dispersible character.
Background technology
There is some algae species that can be used in food, major part is " macro ", such as sea-tangle, sea lettuce (sea lettuce (Ulvalactuca)) and Porphyra (planting in Japan) or " dulse " type (Palma Leah palm leaf (Palmariapalmata) red algae) edible red algae.
But, some algae sources (namely small unicellular alga) represented by " micro-algae " in addition together with these macros, it can be photosynthetic growth or non-photosynthetic growth, has through plantation for the ocean in biodiesel, food, cosmetics or nutrient health or non-marine source.
For example, spirulina (spirulina) (blunt top spirulina (Arthrospiraplatensis)) is planted (under phototrophic conditions) in open lagoon, it is used as dietary supplement ingredient, or marginally mixes (be usually less than 0.5%w/w) in confectionary products or drink.
The micro-algae of other rich lipids (comprising some species of chlorella) is as the welcome of dietary supplement ingredient (can mention that hidden dinoflagellate (Crypthecodinium) or schizochytrium limacinum (Schizochytriumgenus) belong to micro-algae) also pole by Asian countries.
The oil part of chlorella living beings is made up of cholesterol oil substantially, and therefore comparing with polyunsaturated oil with saturated, the hydrogenation be common in conventional food items provides nutrition and healthy advantage.
Chlorella thus with full biomass form or with powder form (obtained by dried pellet algal biomass, its cell membrane is broken by specific mechanical mode) in the food of the mankind or animal consumption.
Microalgae powder also provides other benefits, such as micronutrient, dietary fiber (solubility and insoluble carbohydrate), phosphatide, glycoprotein, phytosterol, tocopherol, tocotrienols and selenium.
For preparation will include the living beings in food composition in, from culture medium concentrated or harvesting biomass (cultivated bioreactor by light autotrophy, or with heterotrophism mode in the dark and cultivate under the carbon source can assimilated by chlorella exists).
In technical field involved in the present invention, the heterotrophic growth of chlorella is preferred (it is called fermentation approach).
When from fermentation medium harvesting microalgae living beings, these living beings comprise the intact cell be mainly suspended in aqueous culture medium.
For concentrated biomass, then by front or tangential flow filtration or by being the known either type centrifugal enforcement solid-liquid separation steps of one of ordinary skill in the art in addition.
After concentration, can directly process microalgae biomass with produce vacuum-packed cake, algae sheet, algae homogenate, complete algae powder, through grinding algae powder or algae oil.
Also by dry for microalgae biomass to promote the subsequent treatment of living beings in various application (particularly food applications) or application.
According to algae bio matter, if drying and drying, according to drying means used, can give the various quality of food product and local flavor.
For example, patent US6607900 describes and uses drum dryer there is no any previously centrifugal dry microalgae biomass that gets off to prepare micro-algae sheet.
Can pneumatic conveyer dryer be used from concentrated microalgae biomass or prepare microalgae powder end, described in patent US6372460 by spraying dry.
Then, in spray dryer, liquid suspension is sparged in heated air flow with the form of small droplet dispersion liquid.Entrained material is rapidly dry and form dried powder.
In other cases, using spraying dry, using the combination of fluidized bed dryer to realize improved conditions (for example, see patent US6255505) for obtaining dry microalgae biomass subsequently.
In technical field involved in the present invention, more specifically seek to prepare the algae powder produced by fermentation approach.
This microalgae powder in background of the present invention is from mechanical lysis and the concentrated microalgae biomass of homogenize (equal pledge is spray-dried or flash drying then) obtains.
The generation of algae powder needs cell lysis to discharge its oil.
For example, stress-breaker can be used to contain the suspension of cell with cell lysis by restricting orifice suction.
Apply high pressure (up to 1500 bar), subsequently through nozzle instantaneous expansion.
Cell breaks by three kinds of different mechanism: the high shear and the outlet pressure bust that enter liquid in valve, orifice plate, thus makes cell explosion.
Molecule in the method release cells.
Buddhist nun sieve (Niro) homogenizer (GEA Ni Luosuoyi (NiroSoavi) or other high pressure homogenisers arbitrary) can be used to process the cell of size mainly between 0.2 micron and 5 microns.
This under high pressure (about 1000 bar) of algae bio matter processes usual cracking and is greater than the cell of 90% and size is reduced to and is less than 5 microns.
Another is chosen as, and replaces use ball mill.
In ball mill, cell is stirred with suspended state together with pellet shapes particle.Breaking of cell is by grinding between shearing force, ball and with caused by the collision of ball.
These balls make cell rupture with therefrom release cells content.Being described in (such as) patent US5330913 of suitable ball mill provides.
The suspension of cells of origin is less than with " oil-in-water " emulsion form acquisition particle diameter.
Then this emulsion of spraying dry and eliminate water, thus leaves the dried powder containing cell fragment, intracellular fluid and oil.
But, pole do not wish produce viscosity, flock together and be difficult to flow dried powder, this is because it contains the oil of 10 % by weight, 25 % by weight or even 50 % by weight content of dried powder.
Even think that high lipid content (being greater than 60%) is more difficult to or even possible efficient drying.
Also the wettability of dried biomass powder and water-dispersible problem are not wished in pole, and then it have poor wettability.
For solving the intrinsic difficulty of these rich Lipid emulsions dry, one of ordinary skill in the art follow following two kinds of main paties usually:
-use particular to select to be applicable to the drying device of rich fatty powder;
-use various flowable (such as based on the product of silica) or spray-drying carrier,
-allotment (by being encapsulated)
These two kinds of approach do not repel mutually.
Spray-drying installation
There is some devices for the rich lipid compounds of spraying dry in the prior art.Can be easy to find the explanation about proposed technology and equipment in the literature: such as, Spray Drying Handbook (SprayDryingHandbook), K. Maas spy (K.Masters), especially in it is the 5th edition, published in 1991 and reprinted in 1994, Longman science and technology (LongmanScientific & Technical) (can obtain under British Library (BritishLibrary) or Library of Congress (LibraryofCongress) are in ISBN0-470-21743-X); Or
spraying dry guide (SprayDryManual), 2005 (access websites www.bete.com)
Therefore seen out, after these files of reading, propose solution and can not be entirely satisfactory, such as:
-drying is rich in the milk product of fat (20%-30%), usually will the concurrent spray drying towers of spray nozzle be equipped with to be used for (second stage is assigned as regulating and cool the powder obtained in the first phase) in two benches device.
But be easy to form deposit and increase by autoxidation mechanism risk on fire, this makes to need to add multiple fire extinguishing system.
-for the cheese of drying process, cheese grinding is mixed with water to form level and smooth emulsifiable paste, then spraying dry.Then in conical bottom spray dryer, spraying dry is implemented.
But, equally because of high fat content, form deposit.
To propose solution be use to be equipped with and to be installed on the fluid bed of spray dryer room pedestal or the spray dryer of moving belt.
But, still there is the problem of the thus obtained dried powder of pneumatic transfer.
-for the non-newborn ice cream of drying (wherein plant fat replaces cream, and casein sodium replaces milk solids non-fat), be difficult to spraying dry due to sugared content higher (being usually greater than 30%).
Therefore need to implement spraying dry under part sugar exists, and carry out supplementary composite by adding fine sugar in drying composite.
In addition, for overcoming the problem of high sugared content, needing the generation that can control dust, the pneumatic transfer of management and control powder, limiting it and reuniting and the deposit avoiding spraying dry chamber interior.
But, only propose to be used in the spray drying chamber being equipped with fluid bed, carry out dry solution.
-for the dry products with 35% to 80% fat, the problem that must avoid is that the diaphragm of lipid globule (especially protein) breaks, and breaks and makes during drying to discharge described fat.
Recommend solution for increasing lipid fusing point, formed at the pedestal place of spray drying chamber or integrate the spray dryer of cooling bed system.
Another is chosen as, and cools the air introduced at the pedestal place of described spray drying chamber to prevent the powder smelting in room by secondary air, and in order to the surface of cured granulate before arbitrary mechanically actuated.
Equally, another is chosen as, if provide cyclone separator for collection powder, then needs before arbitrary collection, to introduce cold air to prevent in the fusing of cyclone separator inside.
Finally, preferably implement combination and comprise the spray drying tower of nozzle and the complex configurations integrating fluid bed or moving belt.
-for dry algae,
The drying of whole micro-algae is especially described in document as described above, this slightly algae be chlorella and Spirullina.
Then its powder type is intended for use to manufacture the tablet for the preparation of the food supplement in nutrition.
Then in the turbine spray dryer being equipped with open loop concurrent spray hothouse, spraying dry is implemented to the living beings with low solid content (10%-15%).
Due to this low solid content, then only produce the powder with fine particle size.
Dry additive
In the field of coffee/tea bleaching agent, these are compositions of combination casein sodium, corn syrup, plant fat and emulsifying agent, potassium phosphate and sodium aluminium silicate.
Spraying dry is implemented in the two benches concurrent spray drier being equipped with oscillatory type external fluidized bed.
Produce the particle with fine particle size.
For obtaining agglomerated particle, select the drier integrating fluid bed and moving belt.
In vegetable oil field, dry olive needs to use spray-drying carrier (such as maltodextrin) in addition.
Summary of the invention
Therefore, still there are the unsatisfied needs of the rich lipid microalgae biomass powder to new stable form, can easily be introduced on a large scale in the food product that must keep delicious and nutrition.
Applicant company finds thus, and this needs to flow extremely significantly by proposing to have for given domain size distribution and given compressed value, the rich lipid microalgae powder of wettability and water-dispersible character meets.
In other words, rich lipid microalgae powder of the present invention there is the rich lipid microalgae powder of the standard that is equivalent to but with significantly flow, particle diameter that wettability is relevant with water dispersible character and compression property.
Therefore, microalgae powder of the present invention (size of powder particles be diameter between 30 μm and 150 μm and described powder has compressibility between 45% and 55%, thin river (HOSOKAWA) powder tester is measured) feature be that it has following characteristics:
-according to test A for 2000 μm oversize measure slamp value, described slamp value between 55 % by weight and 60 % by weight,
-the dispersibility that represented by following item according to test b and wettability:
The height of ■ institute's decant product in beaker, the value of described height is between 0mm and 2mm;
■ degree of wetting, the value of described degree of wetting be greater than total powder 70%, be preferably more than 80%.
Preferably, micro-algae is Chlorella, is preferably Chlorella protothecoides.
In addition, microalgae powder and especially microalgae biomass comprise the lipid of at least 10 dry weight %, 20 dry weight %, 30 dry weight %, 40 dry weight %, 50 dry weight % or 60 dry weight %.
In addition, microalgae powder of the present invention can obtain by using the technique be coupled to for using low-pressure air to purge the flat spray dryer technology of the air broom of spray drying chamber.
As hereafter shown, this drying performs especially modestly to obtain powder of the present invention in following:
-from the flow velocity of the main dry air of flat spray dryer to the ratio of the flow velocity of the air from air broom,
-from the temperature of the air of air broom.
Therefore, the present invention relates to the technique preparing microalgae powder of the present invention, it is characterized in that it comprises:
1) prepare the emulsion of rich lipid microalgae powder in water, its solids content between 15% dry weight and 50 dry weight %,
2) this emulsion is introduced in high pressure homogenisers,
3) this emulsion of spraying in flat spray dryer (being equipped with the air broom for using low-pressure air to purge spray drying chamber in its underpart), carry out regulating to guarantee simultaneously:
A) temperature of main dry air is between 160 DEG C and 240 DEG C,
B) air themperature in air broom part be the highest 70 DEG C, preferably the highest 65 DEG C, more preferably between 50 DEG C and 60 DEG C,
C) ratio from the flow velocity of the air of air broom and the flow velocity of main dry air is greater than 1/3, preferably between 1/3 and 1/2,
D) temperature of cooling-air is between 25 DEG C and 35 DEG C, makes the powder leaving spray dryer have temperature between 60 DEG C and 90 DEG C.
4) thus obtained microalgae powder is collected.
Preferably, micro-algae is Chlorella, preferably Chlorella protothecoides.In addition, micro-algae and especially microalgae biomass comprise the lipid of at least 10 dry weight %, 20 dry weight %, 30 dry weight %, 40 dry weight %, 50 dry weight % or 60 dry weight %.
The invention still further relates to the microalgae powder obtained by present invention process.
The purposes of powder in food sectors that the invention still further relates to powder of the present invention or obtained by present invention process.Specifically, it relates to the method preparing food composition, and it comprises and being added in the composition of food composition by described microalgae powder.
Detailed description of the invention
The present invention relates to the microalgae biomass being rich in nutrients, especially lipid being applicable to human consumption thus.
More specifically, the present invention relates to the microalgae powder can included in food product, wherein the oil content of microalgae powder can make it possible to completely or partially to replace oil to be present in product in conventional food and/or fat.
For the object of the invention, term " microalgae powder " means to break particularly by mechanical system the desciccate of cell membrane of microalgae biomass.
For the object of the invention, consider that micro-algae is the species producing suitable triglyceride oil and/or TL.
Microalgae biomass comprises the oil of at least 10 dry weight % or lipid, preferably at least 25 dry weight % to 35 dry weight % or higher oil or lipids.
More preferably, living beings contain at least 40 dry weight %, at least 50 dry weight %, the oil of at least 75 dry weight % or lipid.
Preferred micro-algae of the present invention can (using sugar as carbon source and do not exist under light) growth in heterotrophism condition.
The micro-algae of rich lipid of applicant company recommendations for selection Chlorella.
Micro-algae used can be selected from Chlorella protothecoides nonexhaustive, Kessler chlorella (Chlorellakessleri), small chlorella (Chlorellaminutissima), Chlorella (Chlorellasp.), heat resistance chlorella (Chlorellasorokiniama), yellowish green chlorella (Chlorellaluteoviridis), chlorella vulgaris (Chlorellavulgaris), Rui Shi chlorella (Chlorellareisiglii), chlorella ellipsoidea (Chlorellaellipsoidea), addicted to sugared chlorella (Chlorellasaccarophila), Kessler class chlorella (Parachlorellakessleri), Bai Shi class chlorella (Parachlorellabeijerinkii), large-scale former algae (Protothecastagnora) and the former algae of mulberry fruit shape (Protothecamoriformis).Preferably, micro-Trentepohlia that the present invention is used is in Chlorella protothecoides species.
Micro-algae is cultivated to produce living beings thus in liquid medium within.
According to the present invention, cultivate micro-algae there is not (heterotrophism condition) under light in containing the culture medium of Carbon and nitrogen sources.
Solid and liquid growth media have in the literature usually, and the recommendation way that preparation is applicable to the defined medium of many microbial strains is found in (such as) www.utex.org/ (website of being safeguarded by The University of Texas at Austin, for its algal cultures preservation (UTEX)) on the net.
Living beings are produced in fermentor (or bioreactor).
The instantiation of bioreactor, condition of culture and heterotrophic growth and propagation method can be combined to improve the efficiency of micro algae growth and lipid by arbitrary appropriate ways.
In technical field involved in the present invention, seek preparation algae powder.
This microalgae powder in background of the present invention is from mechanical lysis and the concentrated microalgae biomass of homogenize (then dry all pledges) obtains.
The feature of microalgae powder of the present invention (particle diameter measure between 30 μm and 150 μm and on the powder tester of thin river compressibility between 45% and 55%) is that it has following characteristics:
-according to test A for 2000 μm oversize measure slamp value, described slamp value between 55 % by weight and 60 % by weight,
-the dispersibility that represented by following item according to test b and wettability:
The height of ■ institute's decant product in beaker, the value of described height is between 0mm and 2mm;
■ degree of wetting, the value of described degree of wetting be greater than total powder 70%, be preferably more than 80%.
In technical field involved in the present invention, microalgae powder has and is typically found at by the particle diameter in the dry chlorella microalgae powder of conventional route (single-action spraying dry) and compression parameters:
-microalgae powder of the present invention has the particle diameter between 30 μm and 150 μm.
This measurement exists according to manufacturer's code (in " small footprint modular operational manual (SmallVolumeModuleOperatinginstruction) ")
lS laser diffraction analyzer is implemented, and this apparatus preparation has its small size dispersed modules or SVM (125ml).
The compressibility that-microalgae powder of the present invention is measured on the powder tester of thin river is between 45% and 55%.
Compression or compressibility values C be according to following equation by calculate aerated bulk density value (=A) to bulk density value (=B) (itself be use the equipment sold with trade name powder tester type PTE by Xi Chuan company by be applied in operational manual recommend the method for measuring aerated bulk density and bulk density to measure) ratio obtain:
By contrast, the compressed value being carried out dry microalgae powder by single-action spraying dry is about 47%.
But amazing and unexpectedly, the feature of microalgae powder of the present invention is its significantly flowing, wettability and dispersibility character.
The flowing property of-microalgae powder of the present invention (measuring according to test A) is better than those for person measured by the microalgae powder by conventional route drying.
Test A comprises the cohesion degree measuring microalgae powder.
This cohesion test is the inspiration by the cohesion test described in the operational manual of the powder characteristics tester type PTE of thin river sold.
It is that the sieve of 800 μm sieves microalgae powder of the present invention that test A is mainly included in mesh opening.
Then reclaim size be less than the powder part of 800 μm and be placed in closed container, and use Te Bula (Turbula) laboratory blender type T2C to be mixed by epicylclic motion.
By this mixing, according to himself characteristic, the tendency that performance is reunited or repelled by microalgae powder of the present invention.
Then the powder mixed in this way is deposited on 2000 μm of sieves and is used for another screening operation.
Once screening terminates, then quantize the oversize on this sieve, and result gives the explanation of " cohesion " or " thickness " character to microalgae powder.
Therefore, free mobility powder (not having coherency thus) in fact can not only be had this Screen Out of wide opening.
The program is as described below:
The product that the product that o sieves aequum on 800 μm of sieves is less than 800 μm to reclaim 50g size,
The powder that the size of these 50g is less than 800 μm by o is placed in volume and is the glass jar (with reference to BVBL Wei Laili-Villeurbanne plug company (VerrerieVilleurbannaise)-Villeurbanne, France (Villeurbanne)) of 1 liter and the lid that closes
This tank is placed in the blender (Te Bula that speed is adjusted to 42rpm by o, T2C type, Wei Li (Willy) A. Ba Huofen Co., Ltd (BachofenSarl)-Suo Ximu (Sausheim)-France) and mix 5 minutes
O prepares sieve (Joseph Salas (Saulas) brand-diameter 200mm be placed on sifter (not Ritchie (Fritsch), model pul Vista (Pulverisette) 00.502 type); Pendant Xi Kesidong (PaisyCosdon)-France); This sub-assembly from bottom to the details at top: sifter, sieve bottom, 800 μm of sieves, 2000 μm of sieves, sifter lids.
O makes the powder deposition be mixed to get on the top (2000 μm of sieves) of this post, and close sifter lid sieve 5 minutes with fixed position amplitude 5 on (not Ritchie) sifter,
O weighs the oversize on this sieve.
Microalgae powder has 2000 μm of oversizes between 55 % by weight and 60 % by weight thus.
By comparison, the slamp value of conventional microalgae powder is about 71%.
-microalgae powder of the present invention has extremely significant dispersibility and wettability character.
This dispersibility and this wettability are represented by the following according to test b:
The height with the value between 0mm and 2mm of o institute's decant product in beaker;
O has and is greater than 70% of total powder, is preferably more than the degree of wetting of the value of 80%.
This amazing and beat all feature is based on the following fact: compressibility and flow measurement display, microalgae powder of the present invention keeps having coherency as conventional microalgae powder, this is because after mixing (it does not use a large amount of mechanical energy (sieving time is only 5min)), 55% to 60% is less than the particle of 800 μm still not by 2000 μm of sieves (although large 2 to 4 times of its opening).
Be easy to thus infer, the dispersiveness representing the described powder of described behavior is answered poor and is difficult to use in thus in the preparation recommending uniform ingredients distribution.
Equally, its wettability should be lower.
Wettability is a kind of technological property, and it such as, through being usually used in characterizing the powder of settling flux in water, in dairy processing industry.
That reflects ability (people such as Hao Geerde Soren gloomy (HaugaardSorensen) that powder becomes dipping after water surface deposition, analyze the method (" M é thodesd'analysedesproduitslaitiersd é shydrat é s " [" Methodsforanalyzingdehydratedmilkproducts "]) of evaporated milk product, Buddhist nun sieve A/S (publishing house) Copenhagen (Copenhagen), Denmark (Denmark), 1978), and reflect that powder absorbs ability (Ka Yuete (Cayot) and the Lorient (Lorient) of water at water surface thus, the structure of lactoprotein and technical functionality (" Structuresettechnofonctionsdesprot é inesdulait " [" Structuresandtechnofunctionsofmilkproteins "]) Paris: Ai Late research company (AirlaitRecherches): Tec and Doc, Antoine Lavoisier (Lavoisier), 1998).
Usually, the measurement of this index comprises and measures a certain amount of powder and infiltrate through the time required in water through the Free Surface of water of inactive state.
People (1978) is waited according to Hao Geerde Soren is gloomy:
If " the wettability index " of-powder is less than 20 seconds, then it can be considered " wettable ".
The Swelling Capacity of-powder also should be relevant with wettability.This is because when powder absorbs water, it is swelling gradually.Then, when various component is dissolved or disperses, the structure of this powder disappears.
This Swelling Capacity is expressed as wetting product %.
The factor affecting wettability especially for the existence of the porosity of the existence of large primary granule, the introducing again of meticulous thing, powder density, powder particle and capillarity and air, in the existence of powder particle surface fat and reconstruction condition.
The test b researched and developed by applicant company comprises in this article more specifically considers following factor:
-by measure when powder is placed on water surface after one period of time of contact powder decant height obtain the behavior of microalgae powder powder when contacting with water.
The ability (representing with %) of-its absorption water.
Scheme for this test is as described below:
500ml20 DEG C of demineralization water is placed in 600ml beaker in low form (flying generation that brand (Fischerbrand) FB33114 beaker) by o,
25g microalgae powder powder is placed on water surface by o equably, does not mix,
O observes the behavior of powder time to time change,
O measures the height of the decant product of beaker bottom.
What have low wettability has coherency powder and will remain in liquid surface place, and the powder with better wettability will more easily decant.
Microalgae powder of the present invention has the dispersibility and wettability that represent in the following manner according to test b:
The height with the value between 0mm and 2mm of o institute's decant product in beaker;
O has and is greater than 70% of total powder, is preferably more than the degree of wetting of the value of 80%.
But, by comparison, usually carry out dry microalgae powder by single-action spraying dry and remain in water surface place, and can not fully hydration so that can beaker bottom be decanted into.
Microalgae powder of the present invention can be obtained by following ad hoc fashion: use the flat spray dryer (or FBSD) be coupled to for using low-pressure air to purge the air broom (or AB) of spray drying chamber to implement drying process with atomizing.
Flat spray dryer be generally used for dry rich fat material or hygroscopic products or on more practical significance for lacking space part etc.But, known to applicant company, its be never used from dry usual micro-algae with the air broom one for using low-pressure air to purge spray drying chamber and especially chlorella through crack biomass.
Just be coupled the device of FBSD and AB, especially recommend to be used for dry fruit, plant slurry and fruit juice or even meat extract.
For drying is through cracking microalgae biomass, follow this spraying dry principle, the FBSD being equipped with AB that (such as) is sold by company CE Rogers (Rogers), Ma Ruite-Wo Keer (MarriottWalker), Heng Ningsen food or food engineering company (HenningsenFoodsorFoodsEngineeringCo.) and Han Silai company (HenszeyCo.) can be used.
Amazing and unexpectedly, applicant company is observed thus, carry out dry microalgae powder by using (such as) this FBSD/AB technique to make not only high yield to prepare the product with standard compressible, grain diameter characteristic and flowable, and give its flowing, wettability and water-dispersible character especially unexpectedly, and without the need to using Granulating Bonding Agent or anti-caking agent.
In fact, above-mentioned technique (being such as generally used for the single-action spraying dry of dried biomass or microalgae powder) can not obtain all desirable characteristics.
The technique preparing microalgae powder of the present invention comprises the following steps: thus
1) prepare the emulsion of rich lipid microalgae powder in water, its solids content between 15% dry weight and 50 dry weight %,
2) this emulsion is introduced in high pressure homogenisers,
3) be equipped with in bottom in the FBSD of AB device and it sprayed, make:
A) temperature of main dry air is between 160 DEG C and 240 DEG C,
B) air themperature in air broom part be the highest 70 DEG C, preferably the highest 65 DEG C, more preferably between 50 DEG C and 60 DEG C,
C) ratio from the flow velocity of the air of AB and the flow velocity of main dry air is greater than 1/3, preferably between 1/3 and 1/2,
D) temperature of cooling-air is between 25 DEG C and 35 DEG C, thus the powder leaving spray dryer has the temperature between 60 DEG C and 90 DEG C.
4) thus obtained microalgae powder is collected.
The first step of present invention process comprises the rich lipid microalgae powder emulsion in water of preparation solids content between 15 dry weight % and 50 dry weight %.Specifically, solids content can between 25% and 45%, preferably between 35% and 45%.In addition, the lipid content of microalgae powder or microalgae biomass is preferably minimum at least 10 dry weight %, 20 dry weight %, 30 dry weight %, 40 dry weight %, 50 dry weight % or 60 dry weight %, such as, between 20 dry weight % and 80 dry weight % or between 30 dry weight % and 70 dry weight %.Optionally, the degree of grinding of microalgae biomass can be at least 25% to 75% of cell lysis, and 50%, 85% or 95% of such as cell lysis and preferably 85% or 95%.
As hereafter illustrated, the living beings obtained when fermentation ends will have about 50% lipid content, 10% to 50% fiber, 2% to 15% protein, 30% sugar and 10% starch usually.
Then following steps are implemented to living beings:
-carry out passivation by rapid thermal treatment (HTST process),
-by using aqueous solution dilution and using centrifugal concentrating to wash,
-in grinding in ball grinder, produce " oil-in-water " emulsion thus.
The second step of present invention process comprises to be introduced this emulsion in high pressure homogenisers.
Applicant company is recommended in this emulsion of enforcement in two benches device (such as by Gao Lin (Gaulin) homogenizer of APV sold) and homogenizes, and wherein the pressure of first stage is 160 bar, and the pressure of second stage is 40 bar.
The third step of present invention process is included in bottom and is equipped with in the FBSD of AB device this solution spray, makes:
A) temperature of main dry air is between 160 DEG C and 240 DEG C,
B) air themperature in AB part be the highest 70 DEG C, preferably the highest 65 DEG C, more preferably between 50 DEG C and 60 DEG C,
C) ratio from the flow velocity of the air of AB and the flow velocity of main dry air is greater than 1/3, preferably between 1/3 and 1/2
D) temperature of cooling-air is between 25 DEG C and 35 DEG C, thus the powder leaving spray dryer has the temperature between 60 DEG C and 90 DEG C.
In this way, as hereafter illustrated, main dry air is fixed on to the flow velocity of 2200kg/h, the flow velocity from the air of AB is greater than 750kg/h, preferably between 800kg/h and 900kg/h.
As hereafter also illustrated, these operating conditions make to limit the formation of deposit on spraying dry locular wall and optimization dry yields is greater than 90%, is preferably more than 95%, more preferably 99% thus.
In addition, except the spraying dry effect in FBSD, existing can these parameter of control AB, and this makes to obtain the product with described wettability and water-dispersible character.
The final step of present invention process comprises the thus obtained microalgae powder of final collection.
This microalgae powder will be used in food sectors.Therefore, the purposes of powder in food sectors that the present invention relates to powder of the present invention or obtain by present invention process.Specifically, it relates to the method preparing food composition, and it comprises in the composition or food composition adding described microalgae powder to food composition.Described purposes (such as) is described in patent application case WO2010/045368, WO2010/120923 or US2010/0297296.
Understand the present invention from following Examples by more than you know, described example intends to have illustrative and non-limiting.
Example
Example 1. obtains Chlorella protothecoides microalgae biomass by fermentation
Fermentation process is described in person in patent application case WO2010/120923 roughly completely to change.
The inoculation of precultures of Chlorella protothecoides is used to produce fermentor.Volume after inoculation reaches 9000l.
Carbon source used is carried out the 55%w/w dextrose syrup of sterilizing by Applicative time/temperature scenario.
Fermentation is charging-batch fermentation, regulates glucose flow velocity to maintain residual glucose concentration for 3g/l to 10g/l during this period.
Producing the fermentor time is 4 to 5 days.
When fermentation ends, cell concentration reaches 185g/l.
During the glucose charging phase, restriction culture medium in nitrogen content with allow lipid with 50% amount accumulate.
Fermentation temperature is maintained at 28 DEG C.
Fermentation pH before inoculation is adjusted to 6.8 and then during fermentation regulates and controls this identical value.
By controlling the stirring of throughput, back-pressure and fermentor, dissolved oxygen is maintained at minimum 30% place.
In HTST district, use the scheme heat treatment fermented juice of 1min and 75 DEG C and be cooled to 6 DEG C.
Then decarbonate drinking water is used to utilize the dilution ratio of 6:1 (water/juice) washing biomass and by using this (AlfaLavalFeux) of Alpha-Lavalle-Fick 510 to carry out centrifugally being concentrated to 250g/l (25%DCW " dry cell wt ").
By at 75 DEG C in HTST district heat treatment within 1 minute, carry out washed cell.
For remaining operation, at temperature being maintained at 8 DEG C-10 DEG C.
By using dilution (3:1 (V
water/ V
living beings)) washing biomass and use centrifugal (disk nozzle-type centrifuge) to concentrate to reduce the concentration of interstitial soluble material.
After this step, be approximately 25% in the solids content being separated exit living beings, then by evaporation and concentration to 45%.
Use ball mill type ball mill grinding institute washing biomass, wherein degree of grinding is 95%.
At use potassium hydroxide, pH is adjusted to after 7, coarse " oil-in-water " type emulsion produced in this way is homogenized under stress in two benches height woods homogenizer (be in the first phase 160 bar/be 40 bar in second stage).
The living beings obtained when fermentation ends have about 50% lipid content, 10% to 50% fiber, 2% to 15% protein, 30% sugar and 10% starch usually, and described percentage represents with the dry weight of total living beings.
The dry microalgae powder of example 2. through " oil-in-water " emulsion that homogenizes
In following equipment dry obtain in example 1 through the emulsion that homogenizes:
-single-action the spray dryer (by the liquid of single-action spray dryer drying by hot-fluid, being then recovered at the bottom of the tower of cyclone separator or sleeve filter) sold by GEA Niro A/S, thus obtain commercially available contrast microalgae powder.
Or
-the flat spray dryer of inside air broom for using low-pressure air to purge spray drying chamber is equipped with, thus obtain microalgae powder of the present invention,
Contrast single-action spray-drying operation condition is as described below:
-input temp is 160 DEG C,
The temperature of-dry section is 60 DEG C,
-Air flow temperature is: 21 DEG C
-output temperature: 60 DEG C.
For drying process with atomizing of the present invention, it is included in be equipped with in the flat spray dryer of air broom and sprays to high pressure homogenizing suspension in the following manner:
-feed system: the head tank with ribbon mixer and heating jacket; Single-screw (single screw) pump; Multiple filter
-spray dryer: centrifuge, diameter is 160mm,
-powder discharge device: for avoiding the whirligig of reuniting bottom room,
-delivery air: the air being loaded with particle leaves room in bottom; Dried powder is separated in sleeve filter with air.
As described below for performing spray-dired parameter in flat spray dryer:
-charging: there is 20% solid and temperature is the emulsion of 50 DEG C-65 DEG C
-turbine, 16400rpm
-emulsion flow velocity: 60kg/h-160kg/h
-major air:
O flow velocity: 2200kg/h
O temperature: 165 DEG C-220 DEG C.
Produce for using air (air broom) to perform the two kinds of configurations purged:
1. standard performs,
2. optimization performs, in order to produce microalgae powder of the present invention
Standard for using air (air broom) to purge (according to manufacturer specification) performs (for 1 test) as described below:
-air velocity: 700kg/h, temperature is 70 DEG C
Output temperature bottom-room: 95 DEG C
-cooling-air: 600kg/h, temperature is 30 DEG C
-air themperature before sleeve filter: 81 DEG C
Production efficiency is herein <90% and observes to form deposit on the wall of spray drying chamber.
The execution purged with the air (air broom) obtaining microalgae powder of the present invention by applicant company optimization (implementing test in triplicate) as described below:
-air velocity: 850kg/h, temperature is 650 DEG C
-cooling-air: 800kg/h, temperature is 30 DEG C.
Production efficiency is herein 99% and does not observe to form deposit on the wall of spray drying chamber.
The sign of example 3. microalgae powder of the present invention
In the following table, following parameters value is presented:
-particle diameter,
-compressibility,
-flowable, by cohesion test (2000 μm)
-wettability,
-water-dispersible
For " single-action spraying dry " contrast, for unoptimizable " air broom " contrast with for three batches of microalgae powders of the present invention.
Completely logically, according to observations, be characterised in that the conventional microalgae powder (" single-action spraying dry " contrasts) of " coherency " particle can not become abundant hydration there is decant, and microalgae powder of the present invention is easy to realize this situation, no matter how it condenses feature.Therefore, conventional microalgae powder is deposited on water surface and can not penetrates in water.In contrast, the abundant hydration of microalgae powder of the present invention and not decant in beaker bottom.
For standard " air broom " test, according to observations, obtain powder not there is any wettability or water-dispersible.
Claims (8)
1. a microalgae powder, the size of this powder particles be diameter between 30 μm and 150 μm and the compressibility measured on the powder tester of thin river of described powder between 45% and 55%, the feature of this microalgae powder is that it has following characteristics:
-the slamp value that measures for the oversize of 2000 μm according to test A, described slamp value between 55 % by weight and 60 % by weight,
-the dispersibility that represented by following item according to test b and wettability:
The height of ■ institute's decant product in beaker, the value of described height is between 0mm and 2mm;
■ degree of wetting, the value of described degree of wetting be greater than total powder 70%, be preferably more than 80%.
2. microalgae powder as claimed in claim 1, the feature of this microalgae powder be this slightly algae be Chlorella (Chlorella), preferably Chlorella protothecoides (Chlorellaprotothecoides).
3. the microalgae powder required by claim 1 or 2, the feature of this microalgae powder is that it comprises the lipid of at least 10 dry weight %.
4. prepare a method for microalgae powder required any one of Claim 1-3, the method is characterized in that it comprises:
1) prepare the emulsion of rich lipid microalgae powder in water, it has the solids content between 15% dry weight and 50 dry weight %,
2) this emulsion is introduced in high pressure homogenisers,
3) being equipped with in bottom for using low-pressure air to purge in the flat spray dryer of the air broom of spray drying chamber this emulsion of spraying, carrying out regulating to guarantee simultaneously:
A) temperature of main dry air is between 160 DEG C and 240 DEG C,
B) air themperature in this air broom part be the highest 70 DEG C, preferably the highest 65 DEG C, more preferably between 50 DEG C and 60 DEG C,
C) ratio from the flow velocity of the air of this air broom and the flow velocity of main dry air is greater than 1/3, preferably between 1/3 and 1/2,
D) temperature of cooling-air is between 25 DEG C and 35 DEG C, makes the powder leaving this spray dryer have temperature between 60 DEG C and 90 DEG C,
4) thus obtained microalgae powder is collected.
5. method as claimed in claim 4, the method is characterized in that this rich lipid microalgae powder comprises the lipid of at least 10 dry weight %.
6. a microalgae powder, this microalgae powder is obtained by the method as described in claim 4 or 5.
7. the purposes of powder in food sectors of the powder according to any one of Claim 1-3 or 6 or the acquisition of the method according to claim 4 or 5.
8. prepare a method for food composition, the microalgae powder that the method comprises microalgae powder required any one of Claim 1-3 or 6 or the method according to claim 4 or 5 obtain adds in the composition of food composition.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR1357110 | 2013-07-19 | ||
| FR1357110A FR3008581B1 (en) | 2013-07-19 | 2013-07-19 | LIPID RICH MICROALGUE FLOUR AND PROCESS FOR PREPARING THE SAME |
| PCT/FR2014/051841 WO2015007999A2 (en) | 2013-07-19 | 2014-07-17 | Lipid-rich microalgal flour and method for preparing same |
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| CN105392374A true CN105392374A (en) | 2016-03-09 |
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| US (1) | US11559074B2 (en) |
| EP (1) | EP3027053B1 (en) |
| JP (1) | JP6474803B2 (en) |
| KR (1) | KR20160030516A (en) |
| CN (1) | CN105392374A (en) |
| BR (1) | BR112016000884B1 (en) |
| DK (1) | DK3027053T3 (en) |
| ES (1) | ES2717439T3 (en) |
| FR (1) | FR3008581B1 (en) |
| MX (1) | MX2016000635A (en) |
| WO (1) | WO2015007999A2 (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR3008581B1 (en) | 2013-07-19 | 2016-11-04 | Roquette Freres | LIPID RICH MICROALGUE FLOUR AND PROCESS FOR PREPARING THE SAME |
| DK3082459T3 (en) | 2013-11-29 | 2019-04-23 | Corbion Biotech Inc | GRANULATES OF MICROALGE BIOMASS MULTIPLE PROTEIN AND METHOD OF PRODUCING THEREOF |
| FR3031987B1 (en) * | 2015-01-26 | 2019-05-24 | Corbion Biotech, Inc. | METHOD FOR FRACTIONING COMPONENTS OF A BIOMASS OF MICROALGUES RICH IN PROTEINS |
| US20180000137A1 (en) * | 2015-01-26 | 2018-01-04 | Roquette Freres | Method for preparing a flour of lipid-rich crushed microalgae |
| US10645950B2 (en) | 2017-05-01 | 2020-05-12 | Usarium Inc. | Methods of manufacturing products from material comprising oilcake, compositions produced from materials comprising processed oilcake, and systems for processing oilcake |
| US11839225B2 (en) | 2021-07-14 | 2023-12-12 | Usarium Inc. | Method for manufacturing alternative meat from liquid spent brewers' yeast |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4956386A (en) * | 1980-04-25 | 1990-09-11 | Gist-Brocades N.V. | Pharmaceutical compositions and process for their preparation |
| CN102340995A (en) * | 2009-03-02 | 2012-02-01 | 罗盖特兄弟公司 | Granulated powder containing vegetable proteins and fibres, method for producing same, and use thereof |
| CN102946738A (en) * | 2009-04-14 | 2013-02-27 | 索拉兹米公司 | Novel microalgal food compositions |
Family Cites Families (62)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3962466A (en) * | 1972-11-10 | 1976-06-08 | Dai-Nippon Sugar Manufacturing Co., Ltd. | Method for treatment of microorganisms |
| JPS5955172A (en) | 1982-09-22 | 1984-03-30 | Yamagataya Noriten:Kk | Preparation of processed food using alga as raw material |
| JPS6075244A (en) | 1983-10-01 | 1985-04-27 | Shimadaya Honten:Kk | Production of noodle |
| US5792631A (en) | 1985-07-01 | 1998-08-11 | Dcv, Inc. | Microbial process for the production of ascorbic acid using Chlorella protothecoides |
| US4917915A (en) | 1986-03-06 | 1990-04-17 | Lever Brothers Company | Water-in-oil emulsion spread |
| US4978553A (en) | 1987-11-02 | 1990-12-18 | Jules Silver | Method of making low-fat butter or margarine spread and resulting product |
| US20060094089A1 (en) | 1988-09-07 | 2006-05-04 | Martek Biosciences Corporation | Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids |
| JP3143636B2 (en) | 1991-09-11 | 2001-03-07 | 株式会社サン・クロレラ | Method for disrupting chlorella cell wall by cell rupture |
| DK201791D0 (en) | 1991-12-17 | 1991-12-17 | Boerge Holm Christensen | METHOD OF COATING |
| US5512311A (en) | 1992-07-21 | 1996-04-30 | National Starch And Chemical Investment Holding Corporation | Starch based lipid mimetic for foods |
| US5346716A (en) | 1992-08-28 | 1994-09-13 | Cumberland Packing Corp. | Low calorie low fat butter-like spread |
| JPH078215A (en) | 1993-04-30 | 1995-01-13 | Kawasaki Steel Corp | Marine microalgal food raw material containing docosahexaenoic acid and its production |
| US5508057A (en) | 1995-01-12 | 1996-04-16 | The Procter & Gamble Company | Process of making monomodal nut butters and spreads |
| JP3850903B2 (en) * | 1995-09-01 | 2006-11-29 | 株式会社ツムラ | Swirl type spray dryer |
| JPH09252707A (en) | 1996-03-22 | 1997-09-30 | Media Internatl:Kk | Production of bread |
| US6255505B1 (en) * | 1996-03-28 | 2001-07-03 | Gist-Brocades, B.V. | Microbial polyunsaturated fatty acid containing oil from pasteurised biomass |
| US6151798A (en) | 1996-09-03 | 2000-11-28 | Niro A/S | Process and apparatus for spray drying or spray cooling |
| US6023855A (en) * | 1996-11-27 | 2000-02-15 | The United States Of America As Represented By The Secretary Of Agriculture | Air-assisted wiping device |
| JPH11132A (en) * | 1997-06-12 | 1999-01-06 | Rengo Co Ltd | Chlorophyll preparation and its production |
| US6372460B1 (en) * | 1997-08-01 | 2002-04-16 | Martek Biosciences | DHA-containing nutritional compositions and methods for their production |
| FR2802212B1 (en) | 1999-12-13 | 2002-03-01 | Agronomique Inst Nat Rech | PROCESS FOR OBTAINING A POWDER CONTAINING VIABLE MICROORGANISMS, POWDER OBTAINED ACCORDING TO THIS PROCESS AND DEVICE FOR ITS IMPLEMENTATION |
| ATE374531T1 (en) * | 2000-01-28 | 2007-10-15 | Martek Biosciences Corp | ENHANCED PRODUCTION OF LIPIDS CONTAINING POLYUNSATURATED FATTY ACIDS BY HIGH DENSITY CULTURES OF EUCARIOTIC MICROBE IN FERMENTATION DEVICES |
| US6472002B2 (en) | 2000-12-04 | 2002-10-29 | Joe Nieh | Diet food formula for overweight people and diabetics |
| JP2003250524A (en) * | 2002-02-28 | 2003-09-09 | Wakamoto Pharmaceut Co Ltd | Cell wall-crushed microorganism, method for producing the same, and use thereof |
| JP2004359476A (en) * | 2003-06-02 | 2004-12-24 | Tokuyama Corp | Hydrophobized silica composition |
| US20050136352A1 (en) * | 2003-12-23 | 2005-06-23 | Xerox Corporation | Emulsion aggregation toner having novel rheolgical and flow properties |
| CN100466916C (en) | 2004-08-10 | 2009-03-11 | 青岛聚大洋海藻工业有限公司 | Processing method of seaweed protein feed |
| BRPI0516785A (en) | 2004-12-15 | 2008-09-23 | Unilever Nv | process for the preparation of cubes of broth and seasonings |
| CN101227900A (en) | 2005-05-11 | 2008-07-23 | 高级生物营养公司 | Fish oil in stabilized form |
| DE102006056454A1 (en) * | 2006-11-28 | 2008-05-29 | Bluebiotech International Gmbh | Taste-free, granular nutritional supplement, useful e.g. for preventing osteoporosis and psoriasis, contains synergistic combination of Spirulina, Chlorella and Lithothamnium algae |
| US8030037B2 (en) * | 2007-01-10 | 2011-10-04 | Parry Nutraceuticals, Division Of E.I.D. Parry (India) Ltd. | Photoautotrophic growth of microalgae for omega-3 fatty acid production |
| FR2924126B1 (en) | 2007-11-28 | 2011-04-15 | Roquette Freres | NOVEL PROCESS FOR CULTURING A HETEROTROPIC MICROALGUE |
| US8236535B2 (en) | 2008-04-30 | 2012-08-07 | Xyleco, Inc. | Processing biomass |
| US20100297292A1 (en) | 2008-10-14 | 2010-11-25 | Solazyme, Inc. | Reduced Pigmentation Microalgae Strains and Products Therefrom |
| US20100297296A1 (en) | 2008-10-14 | 2010-11-25 | Solazyme, Inc. | Healthier Baked Goods Containing Microalgae |
| US20100297323A1 (en) | 2008-10-14 | 2010-11-25 | Solazyme, Inc. | Gluten-free Foods Containing Microalgae |
| US20100303957A1 (en) | 2008-10-14 | 2010-12-02 | Solazyme, Inc. | Edible Oil and Processes for Its Production from Microalgae |
| US20100303989A1 (en) | 2008-10-14 | 2010-12-02 | Solazyme, Inc. | Microalgal Flour |
| US20100297295A1 (en) | 2008-10-14 | 2010-11-25 | Solazyme, Inc. | Microalgae-Based Beverages |
| US20130122180A1 (en) | 2008-10-14 | 2013-05-16 | Solazyme, Inc. | Microalgal Food Compositions |
| US20100303990A1 (en) | 2008-10-14 | 2010-12-02 | Solazyme, Inc. | High Protein and High Fiber Algal Food Materials |
| US20100303961A1 (en) | 2008-10-14 | 2010-12-02 | Solazyme, Inc. | Methods of Inducing Satiety |
| US20160324167A1 (en) | 2008-10-14 | 2016-11-10 | Terravia Holdings, Inc. | Novel microalgal food compositions |
| CN104770424A (en) * | 2008-10-14 | 2015-07-15 | 索拉兹米公司 | Food compositions of microalgal biomass |
| US20120128851A1 (en) | 2008-10-14 | 2012-05-24 | Solazyme, Inc | Novel microalgal food compositions |
| US20100297325A1 (en) | 2008-10-14 | 2010-11-25 | Solazyme, Inc. | Egg Products Containing Microalgae |
| US20100297331A1 (en) | 2008-10-14 | 2010-11-25 | Solazyme, Inc. | Reduced Fat Foods Containing High-Lipid Microalgae with Improved Sensory Properties |
| EP2183977A1 (en) | 2008-11-06 | 2010-05-12 | Unilever N.V. | Food product |
| US8709750B2 (en) | 2008-12-15 | 2014-04-29 | Cavitation Technologies, Inc. | Method for processing an algae medium containing algae microorganisms to produce algal oil and by-products |
| FR2942586B1 (en) | 2009-03-02 | 2011-05-13 | Roquette Freres | GRANULATED POWDER CONTAINING PLANT PROTEINS AND MALTODEXTRINS, PROCESS FOR OBTAINING THEM AND USES THEREOF |
| CN102340994B (en) | 2009-03-02 | 2014-10-22 | 罗盖特兄弟公司 | Vegetable milk granulated powder, method for producing vegetable milk, and uses thereof |
| MA34134B1 (en) | 2010-03-01 | 2013-04-03 | Csm Nederland Bv | BAKERY EMULSION WITH REDUCED FAT CONTENT AND USE OF THE EMULSION IN PREPARATION OF LAMINATED PASTE |
| JP5865894B2 (en) | 2010-04-14 | 2016-02-17 | ソラザイム ロケット ニュートリショナルズ， エルエルシー | High lipid microalgae powder food composition |
| JP5868962B2 (en) | 2010-05-28 | 2016-02-24 | ソラザイム, インコーポレイテッドSolazyme Inc | Oils for specific uses produced from recombinant heterotrophic microorganisms |
| WO2012095121A1 (en) | 2011-01-12 | 2012-07-19 | Cargill, Incorporated | Cocoa powder compositions |
| CN102643714A (en) | 2012-03-09 | 2012-08-22 | 广西大学 | Method for extracting microalgal oil by supercritical CO2 isothermal transformation technology |
| WO2014062882A1 (en) | 2012-10-17 | 2014-04-24 | Solazyme Roquette Nutritionals, LLC | Microalgal flour granules and process for preparation thereof |
| EP2724625A1 (en) | 2012-10-26 | 2014-04-30 | Roquette Freres | Microalgal flour granules and process for preparation thereof |
| US10098371B2 (en) | 2013-01-28 | 2018-10-16 | Solazyme Roquette Nutritionals, LLC | Microalgal flour |
| EP2777400A1 (en) | 2013-03-15 | 2014-09-17 | Roquette Freres | Microalgal flour granules and process for preparation thereof |
| FR3008581B1 (en) | 2013-07-19 | 2016-11-04 | Roquette Freres | LIPID RICH MICROALGUE FLOUR AND PROCESS FOR PREPARING THE SAME |
| DK3082459T3 (en) | 2013-11-29 | 2019-04-23 | Corbion Biotech Inc | GRANULATES OF MICROALGE BIOMASS MULTIPLE PROTEIN AND METHOD OF PRODUCING THEREOF |
-
2013
- 2013-07-19 FR FR1357110A patent/FR3008581B1/en active Active
-
2014
- 2014-07-17 BR BR112016000884-7A patent/BR112016000884B1/en active IP Right Grant
- 2014-07-17 MX MX2016000635A patent/MX2016000635A/en active IP Right Grant
- 2014-07-17 WO PCT/FR2014/051841 patent/WO2015007999A2/en active Application Filing
- 2014-07-17 DK DK14790145.8T patent/DK3027053T3/en active
- 2014-07-17 ES ES14790145T patent/ES2717439T3/en active Active
- 2014-07-17 CN CN201480040810.8A patent/CN105392374A/en active Pending
- 2014-07-17 KR KR1020167001324A patent/KR20160030516A/en not_active Application Discontinuation
- 2014-07-17 JP JP2016526682A patent/JP6474803B2/en active Active
- 2014-07-17 EP EP14790145.8A patent/EP3027053B1/en active Active
- 2014-07-17 US US14/905,342 patent/US11559074B2/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4956386A (en) * | 1980-04-25 | 1990-09-11 | Gist-Brocades N.V. | Pharmaceutical compositions and process for their preparation |
| CN102340995A (en) * | 2009-03-02 | 2012-02-01 | 罗盖特兄弟公司 | Granulated powder containing vegetable proteins and fibres, method for producing same, and use thereof |
| CN102946738A (en) * | 2009-04-14 | 2013-02-27 | 索拉兹米公司 | Novel microalgal food compositions |
Non-Patent Citations (1)
| Title |
|---|
| ANON: "BTE Fog Nozzle", 《BETE SPRAY DRY MANUAL》 * |
Also Published As
| Publication number | Publication date |
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| JP6474803B2 (en) | 2019-02-27 |
| MX2016000635A (en) | 2016-11-30 |
| KR20160030516A (en) | 2016-03-18 |
| WO2015007999A2 (en) | 2015-01-22 |
| DK3027053T3 (en) | 2019-04-15 |
| BR112016000884B1 (en) | 2020-11-03 |
| ES2717439T3 (en) | 2019-06-21 |
| EP3027053B1 (en) | 2019-01-02 |
| WO2015007999A3 (en) | 2015-03-26 |
| US20160143337A1 (en) | 2016-05-26 |
| US11559074B2 (en) | 2023-01-24 |
| FR3008581B1 (en) | 2016-11-04 |
| JP2016524909A (en) | 2016-08-22 |
| EP3027053A2 (en) | 2016-06-08 |
| FR3008581A1 (en) | 2015-01-23 |
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