CN105388279B - Biochip production method and device - Google Patents
Biochip production method and device Download PDFInfo
- Publication number
- CN105388279B CN105388279B CN201510713715.6A CN201510713715A CN105388279B CN 105388279 B CN105388279 B CN 105388279B CN 201510713715 A CN201510713715 A CN 201510713715A CN 105388279 B CN105388279 B CN 105388279B
- Authority
- CN
- China
- Prior art keywords
- substrate
- equipment
- operational order
- drying
- crosslinked
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The present invention provides a kind of biochip production method and device, and method includes obtaining operational order, wherein, operational order include spray, drying, point sample, it is UV-crosslinked in two or more；According to the operational order control chip production equipment operation got, wherein, chip production equipment includes sprinkling equipment, drying equipment, chip point sample equipment and UV-crosslinked equipment.Biochip production method and device that the present invention is provided, first by obtaining operational order, then according to the operational order control chip production equipment operation got, multiple operations to substrate is realized a key control, i.e., multiple operational orders of substrate can successively be performed according to setting, in the process of implementation without manual intervention, it is time saving and energy saving, production efficiency is improved, in addition, due to the reduction of artificial degree of participation, the quality of biochip is also improved.
Description
Technical field
The present invention relates to chip manufacturing field, more particularly to a kind of biochip production method and device.
Background technology
Biochip is according to the principle specifically interacted between biomolecule, by biochemical analysis process integration in chip list
Face, so as to realize the high flux quick detection to DNA, RNA, polypeptide, protein and other biological composition.In biochip
In production process, substrate to be processed is fabricated to final biochip, a variety of instrument and equipments are often needed to use to substrate
It is processed, such as chip point sample instrument, drying box, UV-crosslinked instrument.General every kind of instrument is both needed to be entered by relevant staff
Row operation, because the production process of biochip is related to the use of multiple instruments, and each instrument is separate, so production one
Criticize biochip, it usually needs single or multiple staff carry out multi-pass operations, take time and effort, low production efficiency, and by
It is high in artificial participation, the quality of biochip is have impact on to a certain extent.
The content of the invention
The present invention provides a kind of biochip production method and device, the production to solve biochip in the prior art
The artificial participation of process is high, takes time and effort, the problem of low production efficiency.
One aspect of the present invention provides a kind of biochip production method, including：
Obtain operational order, wherein, operational order include spray, drying, point sample, it is UV-crosslinked in two kinds or two kinds with
On；
According to the operational order control chip production equipment operation got, wherein, chip production equipment is set including spray
Standby, drying equipment, chip point sample equipment and UV-crosslinked equipment.
Further, operational order also includes substrate movement, and substrate movement refers to movement of the substrate on flowing water line platform.
Further, obtaining operational order also includes：
Obtain the parameter of operational order, spray flux of the parameter including spray, the temperature of drying, the point sample scheme of point sample, purple
The speed of intensity and the substrate movement of external crosslinking.
Further, it is specially according to the operational order control chip production equipment operation got：
The translational speed of substrate is controlled, or/and
Control sprinkling equipment is sprayed to substrate, or/and
Substrate is dried control drying equipment, or/and
Control chip point sample equipment carries out point sample to substrate, or/and
UV-crosslinked equipment is controlled to carry out substrate UV-crosslinked.
Another aspect of the present invention provides a kind of biochip process units, including：
Acquisition module, for obtaining operational order, wherein, operational order include spray, drying, point sample, it is UV-crosslinked in
Two or more；
Performing module, for according to the operational order control chip production equipment operation got, chip production equipment bag
Include sprinkling equipment, drying equipment, chip point sample equipment and UV-crosslinked equipment.
Further, operational order also includes substrate movement, and substrate movement refers to movement of the substrate on flowing water line platform.
Further, obtaining operational order also includes：
Obtain the parameter of operational order, spray flux of the parameter including spray, the temperature of drying, the point sample scheme of point sample, purple
The speed of intensity and the substrate movement of external crosslinking.
Further, performing module is specifically included：
First implementation sub-module, the translational speed for controlling substrate；
Second implementation sub-module, for controlling sprinkling equipment to spray substrate；
3rd implementation sub-module, for controlling drying equipment that substrate is dried；
4th implementation sub-module, point sample is carried out for control chip point sample equipment to substrate；
5th implementation sub-module, it is UV-crosslinked for controlling UV-crosslinked equipment to carry out substrate.
Biochip production method and device that the present invention is provided, by obtaining operational order, operational order include spray,
Drying, point sample, it is UV-crosslinked in two or more, then according to the operational order control chip production equipment that gets
Operation, chip production equipment includes sprinkling equipment, drying equipment, chip point sample equipment and UV-crosslinked equipment, makes to substrate
Multiple operations can realize a key control, i.e., multiple operational orders of substrate can successively be performed according to setting, in implementation procedure
In without manual intervention, it is time saving and energy saving, improve production efficiency, further, since the reduction of artificial degree of participation, biochip
Quality is also improved.
Brief description of the drawings
The invention will be described in more detail below based on embodiments and refering to the accompanying drawings.Wherein：
Fig. 1 is the schematic flow sheet of the biochip production method according to the embodiment of the present invention one；
Fig. 2 is a structural representation of the biochip process units according to the embodiment of the present invention two；
Fig. 3 is another structural representation of the biochip process units according to the embodiment of the present invention two.
In the accompanying drawings, identical part uses identical reference.Accompanying drawing is not drawn according to actual ratio.
Embodiment
Below in conjunction with accompanying drawing, the invention will be further described.
Embodiment one
The executive agent of the biochip production method of the present embodiment is biochip process units.Fig. 1 is according to this hair
The schematic flow sheet of the biochip production method of bright embodiment one, as shown in figure 1, the biochip production method of the present embodiment
Including：
Step 101, obtain operational order, wherein, operational order include spray, drying, point sample, it is UV-crosslinked in two kinds
Or it is two or more.
Substrate can enable the reagent uniform fold uniformly spilt from spray head in substrate surface by spray.Substrate leads to
The moisture or other hygroscopic waters of substrate surface can be removed by crossing drying.Biological sample is placed on substrate by point sample.Substrate passes through purple
External crosslinking, can be such that biological sample is combined with substrate by covalent bond or other manner.The sequencing of each operational order can
It is adjusted according to actual conditions.
Step 102, run according to the operational order control chip production equipment got, wherein, chip production equipment bag
Include sprinkling equipment, drying equipment, chip point sample equipment and UV-crosslinked equipment.
Wherein, sprinkling equipment can be the equipment with uniform sprinkling reagent function, and drying equipment can be drying box, chip point
Sample equipment can be chip point sample instrument, and UV-crosslinked equipment can be UV-crosslinked instrument, can specifically be selected according to actual conditions, herein not
Limit.
In the present embodiment, by obtaining operational order, then according to the operational order control chip production equipment got
Operation, enables multiple operations to substrate to realize a key control, i.e., can be according to setting successively to multiple operational orders of substrate
Perform, it is time saving and energy saving in the process of implementation without manual intervention, production efficiency is improved, further, since artificial degree of participation
Reduction, the quality of biochip is also improved.
Further, operational order also includes substrate movement, and substrate movement refers to movement of the substrate on flowing water line platform.
Further, obtaining operational order also includes：The parameter of acquisition operational order, spray flux of the parameter including spray,
Dry temperature, the point sample scheme of point sample, UV-crosslinked intensity and the speed of substrate movement.Specifically, spray flux is more
Few relevant with the surface area size and material of substrate, dry temperature is relevant with the spray flux of spray, UV-crosslinked intensity and
Biological sample is relevant, and the spray flux of spray, the temperature of drying and UV-crosslinked intensity can be set according to specific actual conditions
Put.
Point sample scheme refers to the biomolecule dot matrix being bonded to biomolecule on substrate, and this biomolecule dot matrix can be
Distributed rectangular, or circular distribution, can be also other shapes distribution, can specifically be selected according to actual conditions.
Further, it is specially according to the operational order control chip production equipment operation got：
The translational speed of substrate, or/and control sprinkling equipment is controlled to spray substrate, or/and control drying equipment
Substrate is dried, or/and control chip point sample equipment carries out point sample to substrate, or/and UV-crosslinked equipment is controlled to base
Piece carries out UV-crosslinked.I.e. according to the operational order got, corresponding chip production equipment operation is controlled.
Specific embodiment is set forth below to illustrate.
Obtain operational order first, operational order includes substrate movement, spray, drying, point sample, UV-crosslinked, and operation refers to
The execution sequence of order is spray, drying, point sample, UV-crosslinked, dry, next, according to the operational order control chip got
Production equipment is run.Because the chip production equipment for performing each operational order is different, performed so being instructed in aforesaid operations
During, also with the execution of substrate moving operation instruction.Specially biochip process units opens flowing water line platform, base
Movement of the piece on flowing water line platform, translational speed can be adjusted according to actual conditions；It is biological when substrate is moved to spraying zone
Chip production device controls the sprinkling equipment to spray substrate, and spray flux can be adjusted according to actual conditions；Spray
After completion, biochip process units control streamline platform continues to move to substrate, when substrate, which is reached, dries an area,
Biochip process units control drying equipment a pair of substrate is dried, and drying temperature can adjust；When substrate is moved to point sample
Area, biochip process units control chip point sample equipment to substrate carry out point sample, point sample scheme editable, point sample equipment according to
Point sample scheme carries out point sample to substrate, and after completion, substrate is continued to move to, when substrate enters UV-crosslinked area, biochip life
Producing device controls UV-crosslinked equipment UV-crosslinked to substrate progress, and UV-crosslinked intensity can be adjusted according to actual conditions
Section；Substrate is continued to move to, and is reached and is dried 2nd area, and substrate is dried biochip process units control drying equipment two, does
Dry temperature can adjust, and be finally completed the processing to substrate, obtain chip, above-mentioned drying equipment one can be identical with drying equipment two
Drying equipment, or different drying equipment do not limit herein.
In the production process of above-mentioned whole chip, once set operational order and start quite thing chip production device
Afterwards, then without human intervention, multiple operations to substrate are enable to realize a key control, it is time saving and energy saving, production efficiency is improved,
The quality of biochip is also improved.
Embodiment two
The present invention also provides a kind of biochip process units, the biochip production method for performing embodiment one.
Fig. 2 is a structural representation of the biochip process units according to the embodiment of the present invention two, as shown in Fig. 2 biochip is given birth to
Production device includes：Acquisition module 201 and performing module 202.
Wherein, acquisition module 201, for obtaining operational order, wherein, operational order includes spray, drying, point sample, purple
Two or more in external crosslinking.
Performing module 202, for being run according to the operational order control chip production equipment got, wherein, chip life
Producing equipment includes sprinkling equipment, drying equipment, chip point sample equipment and UV-crosslinked equipment.
In the present embodiment, operational order is obtained by acquisition module 201, then performing module 202 is according to the behaviour got
Make the production equipment operation of instruction control chip, multiple operations to substrate is realized a key control, i.e., to the multiple of substrate
Operational order can successively be performed according to setting, time saving and energy saving in the process of implementation without manual intervention, improve production efficiency,
Further, since the reduction of artificial degree of participation, the quality of biochip is also improved.
Further, operational order also includes substrate movement, and substrate movement refers to movement of the substrate on flowing water line platform.
Further, obtaining operational order also includes：
Obtain the parameter of operational order, spray flux of the parameter including spray, the temperature of drying, the point sample scheme of point sample, purple
The speed of intensity and the substrate movement of external crosslinking.
Fig. 3 is another structural representation of the biochip process units according to the embodiment of the present invention two, as shown in figure 3,
Performing module 202 includes the first implementation sub-module 2021, the second implementation sub-module 2022, the 3rd implementation sub-module the 2023, the 4th
The implementation sub-module 2025 of implementation sub-module 2024 and the 5th.
Wherein, the first implementation sub-module 2021, the translational speed for controlling substrate；Second implementation sub-module 2022, is used
Substrate is sprayed in control sprinkling equipment；3rd implementation sub-module 2023, for controlling drying equipment to do substrate
It is dry；4th implementation sub-module 2024, point sample is carried out for control chip point sample equipment to substrate；5th implementation sub-module 2025,
It is UV-crosslinked for controlling UV-crosslinked equipment to carry out substrate.
The biochip process units of the present embodiment is corresponding with the biochip production method embodiment of embodiment one, specifically
The description in embodiment one is can be found in detail, will not be repeated here.
Although by reference to preferred embodiment, invention has been described, is not departing from the situation of the scope of the present invention
Under, various improvement can be carried out to it and part therein can be replaced with equivalent.Especially, as long as in the absence of structure punching
Prominent, the every technical characteristic being previously mentioned in each embodiment can combine in any way.The invention is not limited in text
Disclosed in specific embodiment, but all technical schemes including falling within the scope of the appended claims.
Claims (6)
1. a kind of biochip production method, it is characterised in that including：
Operational order is obtained, wherein, the operational order includes realizing that spray, drying, point sample, UV-crosslinked and substrate are moved
Instruction, the instruction of substrate movement be instigate substrate to be moved on flowing water line platform instruct, the instruction of the spray
Be realize the instructing of on substrate uniform sprinkling reagent, the instruction of the drying is the moisture or other hygroscopic waters for removing substrate surface
Instruction；
With substrate move instruction execution, according to the operational order got successively according to spray, drying, point sample,
Sequential control chip production equipment operation that is UV-crosslinked, drying, wherein, the chip production equipment includes having on substrate
Sprinkling equipment, the moisture of removal substrate surface or drying equipment, the chip point sample of other hygroscopic waters of uniform sprinkling reagent function are set
Standby and UV-crosslinked equipment.
2. biochip production method according to claim 1, it is characterised in that the acquisition operational order also includes：
The parameter of the operational order is obtained, the parameter includes the spray flux, the temperature of the drying, the point of the spray
The speed of the point sample scheme of sample, the UV-crosslinked intensity and substrate movement.
3. biochip production method according to claim 1 or 2, it is characterised in that it is described that the basis is got
Operational order control chip production equipment is run：
The translational speed of the substrate is controlled, or/and
The sprinkling equipment is controlled to spray substrate, or/and
Control the drying equipment that substrate is dried, or/and
The chip point sample equipment is controlled to carry out point sample to substrate, or/and
The UV-crosslinked equipment is controlled to carry out substrate UV-crosslinked.
4. a kind of biochip process units, it is characterised in that including：
Acquisition module, for obtaining operational order, wherein, the operational order includes realizing spray, drying, point sample, ultraviolet friendship
The instruction that connection and substrate are moved, the instruction of the substrate movement is the instruction for instigating substrate to be moved on flowing water line platform；
Performing module, for the execution for the instruction moved with substrate, according to the operational order got successively according to spray
Pouring, drying, point sample, sequential control chip production equipment that is UV-crosslinked, drying are run, wherein, the chip production equipment bag
Include the sprinkling equipment with uniform sprinkling reagent function, remove the moisture of substrate surface or drying equipment, the chip of other hygroscopic waters
Point sample equipment and UV-crosslinked equipment.
5. biochip process units according to claim 4, it is characterised in that the acquisition operational order also includes：
The parameter of the operational order is obtained, the parameter includes the spray flux, the temperature of the drying, the point of the spray
The speed of the point sample scheme of sample, the UV-crosslinked intensity and substrate movement.
6. the biochip process units according to claim 4 or 5, it is characterised in that the performing module is specifically included：
First implementation sub-module, the translational speed for controlling the substrate；
Second implementation sub-module, for controlling the sprinkling equipment to spray substrate；
3rd implementation sub-module, for controlling the drying equipment that substrate is dried；
4th implementation sub-module, for controlling the chip point sample equipment to carry out point sample to substrate；
5th implementation sub-module, it is UV-crosslinked for controlling the UV-crosslinked equipment to carry out substrate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510713715.6A CN105388279B (en) | 2015-10-28 | 2015-10-28 | Biochip production method and device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510713715.6A CN105388279B (en) | 2015-10-28 | 2015-10-28 | Biochip production method and device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105388279A CN105388279A (en) | 2016-03-09 |
| CN105388279B true CN105388279B (en) | 2017-08-11 |
Family
ID=55420793
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510713715.6A Active CN105388279B (en) | 2015-10-28 | 2015-10-28 | Biochip production method and device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105388279B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105928766B (en) * | 2016-07-15 | 2018-10-26 | 上海百傲科技股份有限公司 | Biochip point sample instrument and its print table |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6308750B1 (en) * | 1999-07-20 | 2001-10-30 | Genpak Limited | Microarrayer apparatus |
| CN1447118A (en) * | 2002-03-21 | 2003-10-08 | 中国科学院生物化学与细胞生物学研究所 | Intelligence biochip point sample instrument |
| WO2004099937A2 (en) * | 2003-05-05 | 2004-11-18 | Robodesign International, Inc. | Microarray dispensing with real-time verification and inspection |
| CN1752755A (en) * | 2005-11-01 | 2006-03-29 | 清华大学 | Biochip spotting robot system |
| CN201096780Y (en) * | 2007-06-26 | 2008-08-06 | 上海裕隆生物科技有限公司 | A biologic chip sampling platform |
| CN202700866U (en) * | 2012-07-26 | 2013-01-30 | 江阴迪林生物电子技术有限公司 | Chip cleaning and drying line |
-
2015
- 2015-10-28 CN CN201510713715.6A patent/CN105388279B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6308750B1 (en) * | 1999-07-20 | 2001-10-30 | Genpak Limited | Microarrayer apparatus |
| CN1447118A (en) * | 2002-03-21 | 2003-10-08 | 中国科学院生物化学与细胞生物学研究所 | Intelligence biochip point sample instrument |
| WO2004099937A2 (en) * | 2003-05-05 | 2004-11-18 | Robodesign International, Inc. | Microarray dispensing with real-time verification and inspection |
| CN1752755A (en) * | 2005-11-01 | 2006-03-29 | 清华大学 | Biochip spotting robot system |
| CN201096780Y (en) * | 2007-06-26 | 2008-08-06 | 上海裕隆生物科技有限公司 | A biologic chip sampling platform |
| CN202700866U (en) * | 2012-07-26 | 2013-01-30 | 江阴迪林生物电子技术有限公司 | Chip cleaning and drying line |
Non-Patent Citations (2)
| Title |
|---|
| cDNA microarray technology and its applications.;Charlie C. Xiang, Yidong Chen;《Biotechnolgy Advances》;20001231;第18卷;第38-40页 * |
| Making and reading microarrays.;Vivian G. Cheung et.al.;《Nature Genetics Supplement》;19990131;第21卷;第15页右侧栏-第16页左侧栏第2段，第19页左侧栏第2段，图1 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105388279A (en) | 2016-03-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ATE484594T1 (en) | METHOD AND MEANS FOR REMOVING A SELECTED DNA SEQUENCE | |
| EP2728162A3 (en) | Nozzle and method of making same | |
| EP2392435A3 (en) | Tool handling system and method for manipulating workpieces by means of cooperating manipulators | |
| ATE468921T1 (en) | METHOD FOR AUTOMATICALLY APPLYING AND MONITORING A STRUCTURE TO BE APPLIED TO A SUBSTRATE AND A DEVICE THEREFOR | |
| DE602005017856D1 (en) | Method and system for achieving improved quality and higher throughput for IEEE 802.11B / G and bluetooth devices in simultaneous operation | |
| EP2808114A3 (en) | Process for flux coating braze preforms and discrete parts | |
| EP1167461A3 (en) | Process for conditioning organic pigments | |
| DE602006012906D1 (en) | A method and apparatus for performing handover between core network units in a packet switched network | |
| ATE459091T1 (en) | METHOD FOR REMOVAL A MICROSCOPIC SAMPLE FROM A SUBSTRATE | |
| DE602006020797D1 (en) | Method for adjusting the aberrations of an apparatus for particle beam lithography, Apparatus for particle beam lithography, and method for producing products with this apparatus | |
| CN105388279B (en) | Biochip production method and device | |
| DE60335142D1 (en) | DEVICE AND METHOD FOR CONTROLLING THE REGISTRATION OF PRESSURE STEPS IN A CONTINUOUS | |
| EP3745066A3 (en) | Treatment system and method for treating workpieces | |
| DE10344284A1 (en) | Device and method for the automated performance of laboratory work steps | |
| DE502005010029D1 (en) | Process and repair blade part for BLISK repair or BLISKs new production | |
| EP2835203A3 (en) | Machine tool and method for machining workpieces with at least two separate machining units | |
| CN105772286A (en) | Numerical control wood door paint sprayer | |
| CN104159679A (en) | Industrial machine for bending metallic flat elements | |
| IL290500A (en) | Method for determining process variables in cell cultivation processes | |
| DE112019006577A5 (en) | Device and method for extracting and aspirating active substances, in particular from the cannabis plant | |
| EP2203035A3 (en) | Automated machine tool and method for drilling reference holes in circuit boards | |
| ATE431866T1 (en) | DEVICE AND METHOD FOR CONNECTING WARP THREADS FROM THREAD LAYERS OF DIFFERENT WEAVES | |
| DE50214055D1 (en) | METHOD FOR ADJUSTING BZW. MONITORING OF A PRESSING TOOL AND PRESSING TOOL BZW. PRESS CONTROL DEVICE FOR THIS | |
| EP1912076A3 (en) | Method and device for characterising wafers during manufacture of solar cells | |
| DE502006001130D1 (en) | Handling device and method for handling workpieces |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |