CN105388118A - Detection method of radix astragali fermentation product for preventing and treating low glycosuria - Google Patents
Detection method of radix astragali fermentation product for preventing and treating low glycosuria Download PDFInfo
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- CN105388118A CN105388118A CN201510679333.6A CN201510679333A CN105388118A CN 105388118 A CN105388118 A CN 105388118A CN 201510679333 A CN201510679333 A CN 201510679333A CN 105388118 A CN105388118 A CN 105388118A
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- radix astragali
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
Abstract
The invention discloses a detection method of a radix astragali fermentation product for preventing and treating low glycosuria. The detection method comprises the steps that a reference solution is prepared, and then a to-be-detected sample solution is prepared for standby application; absorbance values under different concentrations are measured under the wavelength of 510 nm, regression is performed by taking the absorbance values as vertical coordinates and taking the concentrations as the horizontal coordinates, and a standard curve is drawn; the content of total flavones in a sample is measured and calculated. According to the detection method, operation is easy and convenient, the advantages of being accurate in precision, good in repeatability, stable in detection and high in recycle rate are achieved, and the content of the total flavones in the radix astragali fermentation product can be precisely detected.
Description
Technical field
The present invention relates to medicine detection technique field, specifically a kind ofly prevent and treat the low Radix Astragali tunning detection method of glycosuria amount.
Background technology
The Radix Astragali is a kind of common Chinese crude drug, general flavone content in the Radix Astragali is higher relative to other medicinal materials, therefore the Radix Astragali has good curative effect to treatment diabetes, obtain general flavone by the Radix Astragali and have a lot of method, there are the methods such as water-boiling method, fermentation, organic extraction, adopt fermentation and organic extracting method can obtain the general flavone of high yield, for the mensuration of the content of general flavone in Radix Astragali sweat, current existing method is too complicated, operate too loaded down with trivial details, and measuring containing accuracy of measurement not high, error is relatively large, detects unstable.
Summary of the invention
The object of the present invention is to provide and a kind ofly prevent and treat the low Radix Astragali tunning detection method of glycosuria amount, to solve the problem proposed in above-mentioned background technology.
For achieving the above object, the invention provides following technical scheme:
Prevent and treat the Radix Astragali tunning detection method that glycosuria amount is low, concrete operation step is as follows:
(1) preparation of reference substance solution
Precision takes control substance of Rutin 13.000mg in 25mL volumetric flask, and add the ethanol that volume fraction is 70%, heating water bath makes dissolving, lets cool, then is diluted to scale with the ethanol that volume fraction is 70%, shakes up, is configured to 0.52mgg
-1reference substance solution;
(2) preparation of testing sample solution
Get in testing sample 1g and round-bottomed flask, adding 10 times amount volume fractions is the ethanol of 95%, soaked overnight, continuous circumfluence extraction 2h in apparatus,Soxhlet's, cooling, filters, and collect filtrate, constant volume is in 100mL volumetric flask, for subsequent use;
(3) investigation of linear relationship
Accurate draw above-mentioned rutin titer 0,0.05,0.1,0.2,0.4,0.8,1.0mL in 10mL color comparison tube, respectively add the 5%NaNO of 0.5mL
2solution, shakes up and places 6min, then add the 10%Al (NO) of 30.5mL
3, shake up and place 6min, then add the 1molL of 4mL
-1naOH solution, last solution volume fraction is the ethanolic solution constant volume of 70%, shake up place 15min; At 510nm wavelength place, using the first pipe solution as blank, record absorbance under variable concentrations, take absorbance as ordinate, concentration is that horizontal ordinate returns, drawing standard curve;
(4) mensuration of general flavone content and calculating in sample
Accurate pipette samples 5.0mL is respectively in 25mL volumetric flask, and adding distil water, to 6mL, measures by standard curve making method, and record each sample absorbance, by formula X=(C × V
0× 25) (V
1× m) calculate general flavone content, general flavone content in X-sample in formula, V
0-sample liquid cumulative volume to be measured, V
1-sample extracting solution measures volume aspirated, and C-checks in rutin concentration liquid to be measured from typical curve, and m-sample liquid to be measured amounts to original crude drug amount.
Compared with prior art, the invention has the beneficial effects as follows: detection method is simple to operation to there is the advantage that precision is accurate, reproducible, detection is stable and the recovery is high, accurately can detect the content of general flavone in Radix Astragali tunning.
Embodiment
Below in conjunction with the embodiment of the present invention, be clearly and completely described the technical scheme in the embodiment of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
Prevent and treat the Radix Astragali tunning detection method that glycosuria amount is low, concrete operation step is as follows:
(1) preparation of reference substance solution
Precision takes control substance of Rutin 13.000mg as in 25mL volumetric flask, and add 70% appropriate amount of ethanol, heating water bath makes dissolving, lets cool, and is diluted to scale, shakes up, be configured to 0.52mgg with 70% ethanol
-1reference substance solution;
(2) preparation of testing sample solution
Get in testing sample 1g and round-bottomed flask, add 10 times amount 95% ethanol, soaked overnight, continuous circumfluence extraction 2h in apparatus,Soxhlet's, cooling, filter, collect filtrate, constant volume is in 100mL volumetric flask, for subsequent use;
(3) investigation of linear relationship
Accurate draw above-mentioned rutin titer 0,0.05,0.1,0.2,0.4,0.8,1.0mL in 10mL color comparison tube, add the 5%NaNO of 0.5mL
2solution, shakes up and places 6min, then add the 10%Al (NO) of 30.5mL
3, shake up and place 6min, then add the 1molL of 4mL
-1naOH, solution 70% ethanolic solution constant volume, shake up place 15min; At 510nm wavelength place, using the first pipe solution as blank, record absorbance under variable concentrations, take absorbance as ordinate, concentration is that horizontal ordinate returns, and drawing standard curve, as shown in table 1, result shows, control substance of Rutin is at 0.026 ~ 0.52mgmL
-1in good linear relationship in concentration range, its typical curve equation is Y=11.467X+0.0096, r=0.9992;
1. Precision Experiment
Get above-mentioned need testing solution, with reference to above-mentioned typical curve method, measure absorbance at 510nm, METHOD FOR CONTINUOUS DETERMINATION 6 times, RSD is 2.14%(n=6), the results are shown in Table 1, result shows that this law precision is good;
Table 1 precision test measurement result
| Number of times 123456 RSD% |
| A 0.6532 0.6247 0.6537 0.6594 0.6321 0.6502 2.14 |
2. repeatability is investigated
Get with a collection of testing sample, by the parallel obtained 6 parts of need testing solutions of testing sample solution preparation method, micrometric measurement absorbance respectively, the average content calculating general flavone is 5.315%, RSD is 0.58%, the results are shown in Table 2, shows that this method repeatability is good;
Table 2 replica test measurement result
| Number of times 123456 RSD% |
| Record content (%) 5.469 5.229 5.367 5.213 5.301 5.311 0.58 |
3. stability test
Get reference substance and testing sample solution place 0 respectively in room temperature, 5,10,20, after 30mim, measure absorbance RSD and be respectively 1.09% and 0.89%, the results are shown in Table 3, show that reference substance and test solution are stable in 30min;
Table 3 stability experiment measurement result
| Time (min) | 0 | 2 | 4 | 8 | 16 | 30 | RSD% |
| Reference substance | 0.4879 | 0.4973 | 0.4971 | 0.4763 | 0.4859 | 0.4702 | 1.09 |
| Test sample | 0.4685 | 0.4477 | 0.4503 | 0.4571 | 0.4465 | 0.4305 | 0.89 |
4. average recovery test
Precision takes the medicinal material 6 parts of known astragalus flavonid content, every part of 1g, and make testing sample solution by step (2) operation, adding distil water is settled to 2mL, is divided into 2 groups, often organizes 3 parts, respectively accurate interpolation 0.5mgmL
-1control substance of Rutin 0.83mL, 1.04mL, 1.25mL, measure by step (3), and calculate the recovery; Test findings, in table 4, shows that this law has the good recovery;
Average recovery test determination result (n=6)
(4) mensuration of general flavone content and calculating in sample
Accurate pipette samples 5.0mL is respectively in 25mL volumetric flask, and adding distil water, to 6mL, measures by standard curve making method, and record each sample absorbance, by formula X=(C × V
0× 25)/(V
1× m) calculate general flavone content, general flavone content in X-sample in formula, V
0-sample liquid cumulative volume to be measured, V
1-sample extracting solution measures volume aspirated, and C-checks in rutin concentration liquid to be measured from typical curve, and m-sample liquid to be measured amounts to original crude drug amount.
To those skilled in the art, obviously the invention is not restricted to the details of above-mentioned one exemplary embodiment, and when not deviating from spirit of the present invention or essential characteristic, the present invention can be realized in other specific forms.Therefore, no matter from which point, all should embodiment be regarded as exemplary, and be nonrestrictive, scope of the present invention is limited by claims instead of above-mentioned explanation, and all changes be therefore intended in the implication of the equivalency by dropping on claim and scope are included in the present invention.
Claims (1)
1. prevent and treat the Radix Astragali tunning detection method that glycosuria amount is low, it is characterized in that, concrete operation step is as follows:
(1) preparation of reference substance solution
Precision takes control substance of Rutin 12.990 ~ 13.010mg in 25mL volumetric flask, and add the ethanol that volume fraction is 70%, heating water bath makes dissolving, lets cool, then is diluted to scale with the ethanol that volume fraction is 70%, shakes up, is configured to 0.52mgg
-1reference substance solution;
(2) preparation of testing sample solution
Get in testing sample 0.99 ~ 1.01g and round-bottomed flask, adding 9 ~ 11 times amount volume fractions is the ethanol of 95%, soaked overnight, continuous circumfluence extraction 2h in apparatus,Soxhlet's, cooling, filters, and collect filtrate, constant volume is in 100mL volumetric flask, for subsequent use;
(3) investigation of linear relationship
Accurate draw above-mentioned rutin titer 0,0.05,0.1,0.2,0.4,0.8,1.0mL in 10mL color comparison tube, respectively add the 5%NaNO of 0.45 ~ 0.55mL
2solution, shakes up placement 5 ~ 7min, then adds the 10%Al (NO) of 30.0 ~ 31.0mL
3, shake up placement 5 ~ 7min, then add the 1molL of 3.5 ~ 4.5mL
-1naOH solution, last solution volume fraction is the ethanolic solution constant volume of 70%, shakes up placement 12 ~ 18min; At 510nm wavelength place, using the first pipe solution as blank, record absorbance under variable concentrations, take absorbance as ordinate, concentration is that horizontal ordinate returns, drawing standard curve;
(4) mensuration of general flavone content and calculating in sample
Accurate pipette samples 4.5 ~ 5.5.0mL is respectively in 25mL volumetric flask, and adding distil water, to 5.0mL, measures by standard curve making method, and record each sample absorbance, by formula X=(C × V
0× 25) (V
1× m) calculate general flavone content, general flavone content in X-sample in formula, V
0-sample liquid cumulative volume to be measured, V
1-sample extracting solution measures volume aspirated, and C-checks in rutin concentration liquid to be measured from typical curve, and m-sample liquid to be measured amounts to original crude drug amount.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106769800A (en) * | 2016-11-14 | 2017-05-31 | 天津市康婷生物工程有限公司 | A kind of method of high pass measurement mescenchymal stem cell quantity |
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2015
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| CN101112220A (en) * | 2007-08-27 | 2008-01-30 | 刘国庆 | Method for preparing feed additive based on natural plant active component biotransformation |
| CN101152240A (en) * | 2007-10-16 | 2008-04-02 | 中国科学院长春应用化学研究所 | Quality detecting method for traditional Chinese medicine of astragalus membranacus |
| CN102579561A (en) * | 2012-03-16 | 2012-07-18 | 甘肃中医学院 | Method for extracting total flavones and total saponins from astragalus |
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| CN106769800A (en) * | 2016-11-14 | 2017-05-31 | 天津市康婷生物工程有限公司 | A kind of method of high pass measurement mescenchymal stem cell quantity |
| CN106769800B (en) * | 2016-11-14 | 2019-03-22 | 天津市康婷生物工程有限公司 | A kind of method of high pass measurement mescenchymal stem cell quantity |
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