CN105372363A - Method for detecting content of diacetyl in tobacco tar of electronic cigarette - Google Patents
Method for detecting content of diacetyl in tobacco tar of electronic cigarette Download PDFInfo
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Abstract
The invention discloses a method for detecting content of diacetyl in tobacco tar of an electronic cigarette. The method comprises the following steps that a to-be-tested sample is provided; a diacetyl alcoholic solution with 3-6 concentration gradients is taken as a standard working solution, and the concentration range of the diacetyl in the standard working solution is from 6 mug/mL to 60 mug/mL; the to-be-tested sample and the standard working solution are subjected to separation detection respectively with the gas chromatography, and a standard diacetyl regression equation is calculated with an external standard method; the area of the chromatographic peak of the to-be-tested sample is taken into the standard diacetyl regression equation, and the diacetyl content in the to-be-tested sample is calculated. According to the method for detecting the content of diacetyl in tobacco tar of the electronic cigarette, the standard diacetyl regression equation is calculated with the external standard method, the area of the chromatographic peak of the to-be-tested sample is taken into the standard diacetyl regression equation, and the diacetyl content in the to-be-tested sample is calculated.
Description
Technical field
The present invention relates to electronic cigarette product physical and chemical inspection technical field, particularly relate to the detection method of diacetyl content in a kind of electronic cigarette tobacco tar.
Background technology
For a long time, electronic cigarette market present production at home, market general layout abroad.State Bureau takes a stand in annual work meeting, pay much attention to the novel tobacco goods research and development such as electronic cigarette, and along with middle cigarette changes electronic cigarette attitude, electronic cigarette policy risk reduces greatly.Under the background that Tobacco Control is increasingly serious, electronic cigarette is as the good substitute of traditional cigarette, and starting in China's demand is Great possibility, and China has the 300000000 thickness people, and the market space is huge.
Electronic cigarette controls smog output quantity and duty by high-tech silicon and airflow sensor device, electronic cigarette tobacco tar is atomized, solution mist containing plant extracts, essence, glycerine and propylene glycol is changed into particulate, pass through pulmonary absorption, spue simulation smoke simultaneously, but but not containing the tar in cigarette and other objectionable constituent, also can not produce second-hand-cigarette and also can not fill the air or curl up in confined space.
For many years, electronic cigarette is with " health care ", " smoking cessation ", " clearing lung-heat " etc. for slogan, and with network for approach of mainly marketing, in CHINESE REGION, sales volume increases.But for the composition measurement of electronic cigarette product, the research of the aspects such as safety evaluation is also little.
Biacetyl may be contained in essence in electronic cigarette tobacco tar.Biacetyl is a spontaneous accessory substance in sweat, and biacetyl has cream fragrance.Therefore, manufacturer usually add biacetyl produce margarine, margarine or similar oil base thing make final products be cream flavour.The butter aroma fragrance of biacetyl may be used for numerous food product and beverage, comprises cream, cheese, Yoghourt, beer and grape wine, and working concentration scope is 2.5ppm ~ 44ppm.Biacetyl is widely used in food as flavoring additives, and European Union and U.S.'s relevant legislation biacetyl are all approved for safe adjuvant, not restriction.The biacetyl existed in food can be taken in trace by people, through esophagus, in stomach, is rapidly absorbed into blood, through the metabolism of NADH or NADPH reductase in liver.Therefore, the mankind by the oral biacetyl taking in trace short-term and long-term in all can not bring risk.But, biacetyl to Exposure of humans with suction be completely not identical.Biacetyl has pungency, can cause nausea, has a headache and vomit after contact, and its steam and air form explosive mixture, and chance naked light, high heat energy cause combustion explosion, and kickback can occur with oxygenant.Biacetyl steam is heavier than air, can be diffused into place quite far away in lower, meets burning things which may cause a fire disaster and draws and strile-back.If meet high heat, in container, pressure increases, and has the danger of cracking and blast.Therefore, the biacetyl controlled in electronic cigarette tobacco tar is necessary.Current bibliographical information is little to the research of diacetyl content in electronic cigarette tobacco tar, and in electronic cigarette tobacco tar, the detection method of diacetyl content also there is no report.
Summary of the invention
Based on this, be necessary the detection method that diacetyl content in a kind of electronic cigarette tobacco tar is provided.
In electronic cigarette tobacco tar, a detection method for diacetyl content, comprises the steps:
Sample to be tested is provided;
Configuration has the alcoholic solution of the biacetyl of 3 ~ 6 concentration gradients as standard working solution, and in described standard working solution, the concentration range of biacetyl is 6 μ g/mL ~ 60 μ g/mL;
Gas chromatography is adopted to be separated detection to described sample to be tested with described standard working solution respectively, obtain the area of the area of the chromatographic peak of described standard working solution and the chromatographic peak of described sample to be tested, according to linear relationship, according to the concentration of biacetyl in the area of the chromatographic peak of described standard working solution and the described standard working solution of correspondence, calculate biacetyl standard regressive method; And
The area of the chromatographic peak of described sample to be tested is brought in described biacetyl standard regressive method, calculates the diacetyl content in described sample to be tested.
In one embodiment, described sample to be tested is the alcoholic solution of electronic cigarette tobacco tar or electronic cigarette tobacco tar, and the solvent of the alcoholic solution of described electronic cigarette tobacco tar is ethanol, propylene glycol or glycerine.
In one embodiment, the solvent described in the alcoholic solution of the biacetyl of 3 ~ 6 concentration gradients is ethanol, propylene glycol or glycerine.
In one embodiment, described standard working solution comprises the alcoholic solution of the biacetyl with 4 concentration gradients, and in the alcoholic solution of the biacetyl of described 4 concentration gradients, the relative ratio of each biacetyl concentration is 1:3:5:10.
In one embodiment, in the alcoholic solution of the biacetyl of described 4 concentration gradients, each biacetyl concentration is respectively 6 μ g/mL, 18 μ g/mL, 30 μ g/mL and 60 μ g/mL.
In one embodiment, described gas chromatography is adopted to be separated being operating as of detection to described sample to be tested with described standard working solution respectively: to adopt gas chromatograph to be equipped with flame ionization ditector and detect described sample to be tested and described standard working solution respectively.
In one embodiment, when adopting gas chromatograph outfit flame ionization ditector to detect described sample to be tested and described standard working solution respectively, the temperature of the chromatographic column of described gas chromatograph is 36 DEG C ~ 42 DEG C, the temperature of described flame ionization ditector is 180 DEG C ~ 220 DEG C, the temperature of the injection port of described gas chromatograph is 190 DEG C ~ 230 DEG C, the carrier gas of described gas chromatograph is inert gas or nitrogen, the constant current flow velocity of described gas chromatograph is 75mL/min ~ 95mL/min, the sample size of described gas chromatograph is 0.8 μ L ~ 1.2 μ L, the split ratio of described gas chromatograph is 0.8 ~ 1.2:10.
In one embodiment, when adopting gas chromatograph outfit flame ionization ditector to detect described sample to be tested and described standard working solution respectively, the temperature of the chromatographic column of described gas chromatograph is 39 DEG C, the temperature of described flame ionization ditector is 200 DEG C, the temperature of the injection port of described gas chromatograph is 210 DEG C, the carrier gas of described gas chromatograph is nitrogen, the constant current flow velocity of described gas chromatograph is 85mL/min, the sample size of described gas chromatograph is 1 μ L, and the split ratio of described gas chromatograph is 1:10.
In one embodiment, the chromatographic column of described gas chromatograph is OV-1701 type capillary chromatographic column.
In one embodiment, described biacetyl standard regressive method is: Y=42546.2X, wherein, Y is the area of the chromatographic peak of described standard working solution, the concentration of biacetyl in the unit of Y to be xx, X be corresponding described standard working solution, the unit of X is μ g/mL.
In this electronic cigarette tobacco tar, the detection method of diacetyl content is by having the alcoholic solution of the biacetyl of 3 ~ 6 concentration gradients as standard working solution, according to the concentration of biacetyl in the area of the chromatographic peak of standard working solution and the standard working solution of correspondence after adopting gas chromatography to detect, calculate biacetyl standard regressive method, the area of the chromatographic peak of sample to be tested is brought in biacetyl standard regressive method, the diacetyl content in sample to be tested can be calculated.
Accompanying drawing explanation
Fig. 1 is the process flow diagram of the detection method of diacetyl content in the electronic cigarette tobacco tar of an embodiment;
Fig. 2 is that in embodiment, concentration is the chromatogram of the biacetyl of the standard working solution of 60 μ g/mL.
Embodiment
For enabling above-mentioned purpose of the present invention, feature and advantage become apparent more, are described in detail the specific embodiment of the present invention below in conjunction with accompanying drawing.Set forth a lot of detail in the following description so that fully understand the present invention.But the present invention can be much different from alternate manner described here to implement, those skilled in the art can when without prejudice to doing similar improvement when intension of the present invention, therefore the present invention is by the restriction of following public concrete enforcement.
In the electronic cigarette tobacco tar of an embodiment as shown in Figure 1, the detection method of diacetyl content, comprises the steps:
S10, provide sample to be tested.
Sample to be tested is the alcoholic solution of electronic cigarette tobacco tar or electronic cigarette tobacco tar.
The solvent of the alcoholic solution of electronic cigarette tobacco tar can be ethanol, propylene glycol or glycerine.
In one embodiment, the alcoholic solution of electronic cigarette tobacco tar can by following operative configuration: accurately take 0.1g electronic cigarette tobacco tar, be placed in 10mL volumetric flask, shake 0.5min with after absolute ethyl alcohol constant volume, obtain the alcoholic solution of required electronic cigarette tobacco tar.
S20, configuration have the alcoholic solution of the biacetyl of 3 ~ 6 concentration gradients as standard working solution.
The solvent with the alcoholic solution of the biacetyl of 3 ~ 6 concentration gradients is ethanol, propylene glycol or glycerine.
In standard working solution, the concentration range of biacetyl is 6 μ g/mL ~ 60 μ g/mL.
Preferably, standard working solution comprises the alcoholic solution of the biacetyl with 4 concentration gradients, and in the alcoholic solution of the biacetyl of 4 concentration gradients, the relative ratio of each biacetyl concentration is 1:3:5:10.
Especially, in the alcoholic solution of the biacetyl of 4 concentration gradients, each biacetyl concentration is respectively 6 μ g/mL, 18 μ g/mL, 30 μ g/mL and 60 μ g/mL.
Standard working solution can adopt by following operative configuration: accurately take 0.03g biacetyl, is placed in 50mL volumetric flask, uses absolute ethyl alcohol constant volume, obtains Standard Stock solutions; Accurately measure the above-mentioned Standard Stock solutions of 0.1mL, 0.3mL, 0.5mL and 1mL respectively in 4 10mL volumetric flasks, use absolute ethyl alcohol constant volume, the alcoholic solution obtaining the biacetyl of 4 concentration gradients is required standard working solution.
S30, employing gas chromatography are separated detection to the sample to be tested that S10 obtains with the standard working solution that S20 obtains respectively, obtain the area of the area of the chromatographic peak of standard working solution and the chromatographic peak of sample to be tested, according to linear relationship, according to the concentration of biacetyl in the area of the chromatographic peak of standard working solution and the standard working solution of correspondence, calculate biacetyl standard regressive method.
Gas chromatography is adopted to be separated being operating as of detection to sample to be tested with standard working solution respectively: to adopt gas chromatograph to be equipped with flame ionization ditector and detect described sample to be tested and described standard working solution respectively.
Preferably, when adopting gas chromatograph outfit flame ionization ditector to detect sample to be tested and standard working solution respectively, the temperature of the chromatographic column of gas chromatograph is 36 DEG C ~ 42 DEG C, the temperature of flame ionization ditector is 180 DEG C ~ 220 DEG C, the temperature of the injection port of gas chromatograph is 190 DEG C ~ 230 DEG C, the carrier gas of gas chromatograph is inert gas or nitrogen, the constant current flow velocity of gas chromatograph is 75mL/min ~ 95mL/min, the sample size of gas chromatograph is 0.8 μ L ~ 1.2 μ L, the split ratio of gas chromatograph is 0.8 ~ 1.2:10, split ratio in the present invention refers to the shunting ratio of each material in detected sample, namely with carrier gas conveying material with not carry with carrier gas and by the ratio of material shunted.
Especially, when adopting gas chromatograph outfit flame ionization ditector to detect sample to be tested and standard working solution respectively, the temperature of the chromatographic column of gas chromatograph is 39 DEG C, the temperature of flame ionization ditector is 200 DEG C, the temperature of the injection port of gas chromatograph is 210 DEG C, and the carrier gas of gas chromatograph is nitrogen, and the constant current flow velocity of gas chromatograph is 85mL/min, the sample size of gas chromatograph is 1 μ L, and the split ratio of gas chromatograph is 1:10.
Preferably, the chromatographic column of gas chromatograph is OV-1701 type capillary chromatographic column (30m × 0.23mm × 0.5 μm).
The measurement of detectability: concentration is respectively alcoholic solution (standard working solution) inject gas chromatograph of the biacetyl of 6 μ g/mL, 18 μ g/mL, 30 μ g/mL and 60 μ g/mL, obtain the range of linearity and linearly dependent coefficient, calculate detectability (LOD) with 3 times of signal to noise ratio (S/N ratio)s (S/N=3), the results are shown in Table 1.
The measurement of repeatability and recovery of standard addition: add in above-mentioned obtained Standard Stock solutions (concentration of biacetyl is 600 μ g/mL) in not containing the blank sample of biacetyl, parallelly do 6 repetitions, and calculate its average recovery rate according to adding scalar sum measured value, result relative standard deviation is less than 5%, explanation precision is good, the results are shown in Table 1.
Table 1: the typical curve of biacetyl, detection limit and the recovery
In table 1, the biacetyl standard regressive method calculated is: Y=42546.2X, and wherein, Y is the area of the chromatographic peak of described standard working solution, and the concentration of biacetyl in the unit of Y to be mV, X be corresponding described standard working solution, the unit of X is μ g/mL.
As can be seen from Table 1, the detection method of diacetyl content in this electronic cigarette tobacco tar, pre-treatment is simple, does not need purification, easy and simple to handle, fast, detection limit (LOD) is 0.68 μ g/g, and recovery of standard addition is 99.5%, and relative standard deviation (RSD) is less than 3.86%, this shows that the detection method of diacetyl content in this electronic cigarette tobacco tar is highly sensitive, repeatability and the recovery good.
S40, the area of the chromatographic peak of sample to be tested obtained by S30 are brought in the biacetyl standard regressive method that S30 obtains, and calculate the diacetyl content in sample to be tested.
In this electronic cigarette tobacco tar, the detection method of diacetyl content is by having the alcoholic solution of the biacetyl of 3 ~ 6 concentration gradients as standard working solution, according to the concentration of biacetyl in the area of the chromatographic peak of standard working solution and the standard working solution of correspondence after adopting gas chromatography to detect, calculate biacetyl standard regressive method, the area of the chromatographic peak of sample to be tested is brought in biacetyl standard regressive method, the diacetyl content in sample to be tested can be calculated.
In addition, in this electronic cigarette tobacco tar, the detection method of diacetyl content is highly sensitive, repeatability and the recovery good.
It is below specific embodiment.
Embodiment 1
Instrument, reagent and instrument condition of work.
Instrument: GC9890B gas chromatograph, joins flame ionization ditector (FID Shanghai Ling Hua Instrument Ltd.), FA2004N electronic balance (MAX:200gd=0.1mg Shanghai Jing Hai Instrument Ltd.)
Reagent: biacetyl (StandardforGC ，≤99.0%GC Aladdin), chromatographically pure ethanol (U.S. Fisher)
GC conditions: chromatographic column: OV-1701 type capillary chromatographic column (30m × 0.23mm × 0.5 μm); Column temperature: 39 DEG C; Detector temperature: 200 DEG C; Injector temperature: 210 DEG C; Carrier gas: nitrogen (purity >=99.999%), constant current flow velocity: 85.0mL/min; Sample size: 1 μ L, split ratio 1:10.
The preparation of Standard Stock solutions: accurately take 0.03g biacetyl (being accurate to 0.0001g), be placed in 50mL volumetric flask, use absolute ethyl alcohol constant volume, calculating concentration is 600 μ g/mL.
The preparation of standard working solution: accurately measure the above-mentioned Standard Stock solutions of 0.1mL, 0.3mL, 0.5mL and 1mL respectively in 10mL volumetric flask, use absolute ethyl alcohol constant volume, namely the biacetyl standard working solution of 4 kinds of variable concentrations is mixed with, series standard working solution concentration is respectively: 6 μ g/mL, 18 μ g/mL, 30 μ g/mL and 60 μ g/mL.
The determination of standard working curve: carry out GC mensuration to standard working solution, makes the typical curve of concentration and peak area ratio and to calculate biacetyl standard regressive method be Y=42546.2X, and typical curve should be linear relationship, coefficient R
2=0.997.
Wherein, concentration be the chromatogram of the standard working solution of 60 μ g/mL as shown in Figure 2.As seen from Figure 2, biacetyl is contained in standard working solution.
The preparation of sample to be tested: sample to be tested is electronic cigarette tobacco tar (HSC-20150916, apple taste), electronic cigarette tobacco tar direct injected.
Result calculates: carry out quantitative test with external standard method, measure sample to be tested, record the area of the chromatographic peak of sample to be tested, substitutes into biacetyl standard regressive method and asks diacetyl content in sample to be tested, does not detect biacetyl in result display sample to be tested.
For the accuracy of determination methods, in sample to be tested, add biacetyl make diacetyl content in this detection sample be 600 μ g/g.Get and detect sample 1.0142g in 10mL volumetric flask, use absolute ethyl alcohol constant volume, sample introduction 1 μ l, recording diacetyl content is 597 μ g/g, and the recovery is 99.5%.
Embodiment 2
Method as described in Example 1, selects another kind of electronic cigarette tobacco tar (HSC-20150916, tobacco taste) as sample, not detect biacetyl.
The above embodiment only have expressed several embodiment of the present invention, and it describes comparatively concrete and detailed, but therefore can not be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (10)
1. the detection method of diacetyl content in electronic cigarette tobacco tar, is characterized in that, comprise the steps:
Sample to be tested is provided;
Configuration has the alcoholic solution of the biacetyl of 3 ~ 6 concentration gradients as standard working solution, and in described standard working solution, the concentration range of biacetyl is 6 μ g/mL ~ 60 μ g/mL;
Gas chromatography is adopted to be separated detection to described sample to be tested with described standard working solution respectively, obtain the area of the area of the chromatographic peak of described standard working solution and the chromatographic peak of described sample to be tested, according to linear relationship, according to the concentration of biacetyl in the area of the chromatographic peak of described standard working solution and the described standard working solution of correspondence, calculate biacetyl standard regressive method; And
The area of the chromatographic peak of described sample to be tested is brought in described biacetyl standard regressive method, calculates the diacetyl content in described sample to be tested.
2. the detection method of diacetyl content in electronic cigarette tobacco tar according to claim 1, it is characterized in that, described sample to be tested is the alcoholic solution of electronic cigarette tobacco tar or electronic cigarette tobacco tar, and the solvent of the alcoholic solution of described electronic cigarette tobacco tar is ethanol, propylene glycol or glycerine.
3. the detection method of diacetyl content in electronic cigarette tobacco tar according to claim 1, is characterized in that, described in there is the alcoholic solution of the biacetyl of 3 ~ 6 concentration gradients solvent be ethanol, propylene glycol or glycerine.
4. the detection method of diacetyl content in electronic cigarette tobacco tar according to claim 1, it is characterized in that, described standard working solution comprises the alcoholic solution of the biacetyl with 4 concentration gradients, in the alcoholic solution of the biacetyl of described 4 concentration gradients, the relative ratio of each biacetyl concentration is 1:3:5:10.
5. the detection method of diacetyl content in electronic cigarette tobacco tar according to claim 4, it is characterized in that, in the alcoholic solution of the biacetyl of described 4 concentration gradients, each biacetyl concentration is respectively 6 μ g/mL, 18 μ g/mL, 30 μ g/mL and 60 μ g/mL.
6. the detection method of diacetyl content in electronic cigarette tobacco tar according to claim 1, it is characterized in that, adopt described gas chromatography to be separated being operating as of detection to described sample to be tested with described standard working solution respectively: adopt gas chromatograph to be equipped with flame ionization ditector and respectively described sample to be tested and described standard working solution are detected.
7. the detection method of diacetyl content in electronic cigarette tobacco tar according to claim 6, it is characterized in that, when adopting gas chromatograph outfit flame ionization ditector to detect described sample to be tested and described standard working solution respectively, the temperature of the chromatographic column of described gas chromatograph is 36 DEG C ~ 42 DEG C, the temperature of described flame ionization ditector is 180 DEG C ~ 220 DEG C, the temperature of the injection port of described gas chromatograph is 190 DEG C ~ 230 DEG C, the carrier gas of described gas chromatograph is inert gas or nitrogen, the constant current flow velocity of described gas chromatograph is 75mL/min ~ 95mL/min, the sample size of described gas chromatograph is 0.8 μ L ~ 1.2 μ L, the split ratio of described gas chromatograph is 0.8 ~ 1.2:10.
8. the detection method of diacetyl content in electronic cigarette tobacco tar according to claim 7, it is characterized in that, when adopting gas chromatograph outfit flame ionization ditector to detect described sample to be tested and described standard working solution respectively, the temperature of the chromatographic column of described gas chromatograph is 39 DEG C, the temperature of described flame ionization ditector is 200 DEG C, the temperature of the injection port of described gas chromatograph is 210 DEG C, the carrier gas of described gas chromatograph is nitrogen, the constant current flow velocity of described gas chromatograph is 85mL/min, the sample size of described gas chromatograph is 1 μ L, the split ratio of described gas chromatograph is 1:10.
9. in the electronic cigarette tobacco tar according to claim 6,7 or 8, the detection method of diacetyl content, is characterized in that, the chromatographic column of described gas chromatograph is OV-1701 type capillary chromatographic column.
10. the detection method of diacetyl content in electronic cigarette tobacco tar according to claim 1, it is characterized in that, described biacetyl standard regressive method is: Y=42546.2X, wherein, Y is the area of the chromatographic peak of described standard working solution, the concentration of biacetyl in the unit of Y to be mV, X be corresponding described standard working solution, the unit of X is μ g/mL.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105938127A (en) * | 2016-07-18 | 2016-09-14 | 中国烟草总公司郑州烟草研究院 | Method for measuring butanedione in smoke liquid of electronic cigarette |
| CN114965778A (en) * | 2022-05-30 | 2022-08-30 | 江西威科油脂化学有限公司 | Method for determining palmitamide and stearamide in stearamide product |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2451330A1 (en) * | 2001-06-20 | 2002-12-27 | Labatt Brewing Company Limited | Combination continuous/batch fermentation processes |
| CN102778527A (en) * | 2012-07-19 | 2012-11-14 | 贵州茅台酒股份有限公司 | Method for measuring content of diacetyl in white spirit |
| CN104169187A (en) * | 2012-03-23 | 2014-11-26 | 第一三共株式会社 | Layered body for ptp or blister pack, and ptp or blister pack |
-
2015
- 2015-10-27 CN CN201510707565.8A patent/CN105372363A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2451330A1 (en) * | 2001-06-20 | 2002-12-27 | Labatt Brewing Company Limited | Combination continuous/batch fermentation processes |
| CN104169187A (en) * | 2012-03-23 | 2014-11-26 | 第一三共株式会社 | Layered body for ptp or blister pack, and ptp or blister pack |
| CN102778527A (en) * | 2012-07-19 | 2012-11-14 | 贵州茅台酒股份有限公司 | Method for measuring content of diacetyl in white spirit |
Non-Patent Citations (3)
| Title |
|---|
| V.MACCIOLA等: "Rapid gas-chromatographic method for the determination of diacetyl in milk,fermented milk and butter", 《FOOD CONTROL》 * |
| 李丽: "气相色谱法分析2,3-丁二酮", 《香料香精化妆品》 * |
| 相启森等: "食品中2,3-丁二酮形成机制和检测方法的研究进展", 《食品与发酵工业》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105938127A (en) * | 2016-07-18 | 2016-09-14 | 中国烟草总公司郑州烟草研究院 | Method for measuring butanedione in smoke liquid of electronic cigarette |
| CN114965778A (en) * | 2022-05-30 | 2022-08-30 | 江西威科油脂化学有限公司 | Method for determining palmitamide and stearamide in stearamide product |
| CN114965778B (en) * | 2022-05-30 | 2023-11-17 | 江西威科油脂化学有限公司 | Determination method of palmitoyl acid amide and stearic acid amide in stearic acid amide product |
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