CN105372221A - Method used for detecting content of ponceau 4R in beverage via fluorescence quenching - Google Patents
Method used for detecting content of ponceau 4R in beverage via fluorescence quenching Download PDFInfo
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- CN105372221A CN105372221A CN201510898873.3A CN201510898873A CN105372221A CN 105372221 A CN105372221 A CN 105372221A CN 201510898873 A CN201510898873 A CN 201510898873A CN 105372221 A CN105372221 A CN 105372221A
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Abstract
The invention discloses a method used for detecting content of ponceau 4R in beverage via CdTe quantum dot fluorescence quenching. According to the method, a CdTe quantum dot probe is taken as a fluorescent probe, high selectivity and high sensitivity detection is carried out based on the characteristic that fluorescence intensity of the CdTe quantum dot probe is reduced along with increasing of the concentration of ponceau 4R; excellent linear relation of the changing value of the fluorescence intensity of the CdTe quantum dot probe with the concentration of ponceau 4R is shown, and the square of correlation coefficient is 0.995. The method is simple in operation, high in sensitivity and selectivity, and rapid in detection, and is suitable for on-site in-situ analysis.
Description
Technical field
What the present invention proposed is a kind of method detecting carmine content in beverage of design.
Background technology
Famille rose, having another name called No. 102, edible redness of the skin or complexion (Japan), edible No. 7, redness, ponceau 4R, bright red, brilliant scarlet G, is aqueous solution azo-based colorant.Chemical name is 1-(4'-sulfonic group-1'-naphthylazo)-beta naphthal-6,8-disulfonic acid trisodium salt is the isomeride of amaranth.Carmine (Ponceau4R) is a kind of monoazo class synthetic food color that current China uses the most extensively, consumption is maximum.The carmine material through metabolism generation beta-naphthylamine and alpha-amido-1-naphthols etc. in vivo as a kind of azo-compound with strong carcinogenicity, carmine forbid with EU criteria Sudan redly belong to azo pigment, azo-compound metabolism can generate mutagen precursor in vivo, aromatic amine compounds, aromatic amine is become electrophilic product and is combined with DNA and RNA and forms adduct and Mutation induction after further metabolism activation.The U.S., Canada, Norway forbid adding in food carmine, and the states such as China, European Union, Japan allow interpolation famille rose in food, but all there are strict requirements to its usable range and the highest use amount.According to the regulation in the mensuration (GB2760-2011) of synthetic coloring matter in National Standard of the People's Republic of China's food, for allowing to add in carmine food, carmine detectability is 0.025 0.5g/kg.
Current many methods are used to detect carmine content, as high performance liquid chromatography (HPLC), the electrochemical method of different modifying electrode, mass spectrum (MS), diode array detector (DAD), electrophoresis, Surface enhanced raman spectroscopy (SERS), polarography, spectrophotometric method, competitive Enzyme linked immunosorbent assay (icELISA) etc.Although these methods are all generally acknowledged and widely accepted methods, relatively costly equipment, need dedicated technician to carry out operating, loaded down with trivial details sample pre-treatments and operating cost time etc. shortcoming limit its application.
The present invention, utilizes water-soluble CdTe quantum dots to detect content carmine in beverage as fluorescence probe, and the method has the advantages such as simple, quick, cheap, high sensitivity and high selectivity, to guarantee food security and consumers' rights and interests significant.
Summary of the invention
1 synthesis quantum dot.
2 set up working curve: the standard solution that the concentration configuring a series of famille rose increases gradually, the CdTe quantum of identical amount is added in every part of solution, CdTe quantum is utilized to detect the content of the famille rose in solution as fluorescence probe, the linear relationship between the fluorescence intensity of CdTe quantum and carmine concentration is drawn, i.e. working curve from fluorescence spectrum figure.
3 detect: joined by analysis sample in CdTe quantum solution, make the concentration of CdTe quantum identical with the concentration in above-mentioned each part standard solution, detect the fluorescence intensity of this analytical sample solution, according to described working curve, determine content carmine in analytical sample.
Accompanying drawing explanation
Fig. 1 is working curve.
Embodiment
1 synthesis quantum dot: first in 2mL aqueous solution, appropriate sodium borohydride and 2.9mg tellurium powder hybrid reaction are obtained the solution 1 of sodium hydrogen telluride; In there-necked flask, add 100mL water, add a certain amount of caddy and regulate pH=11.4(to be equivalent to quantum dot concentration 2 × 10
-4mol/L), under 100 μ L mercaptopropionic acid stabilizing agent existence conditions, fresh obtained solution 1 is added in this solution, heating.Under normal temperature, take rhodamine 6G as contrast, record the quantum efficiency of synthesized quantum dot about ~ 25%.
2 set up working curve: get 11 color comparison tubes, add 1mlCdTe quantum dot respectively, add cochineal solution (0mg/mL successively, 0.1mg/mL, 0.2mg/mL, 0.3mg/mL, 0.4mg/mL, 0.5mg/mL, 0.6mg/mL, 0.7mg/mL, 0.8mg/mL, 0.9mg/mL, and 1mg/mL) be settled to 4mL(quantum dot concentration 2 × 10 with the PBS buffer solution that pH value is 7.4
-4mol/L), incubated at room temperature, after 5 minutes, records fluorescence F with fluorospectrophotometer
0-F
11.With lg (F
0/ F) be ordinate, carmine concentration is horizontal ordinate mapping (as shown in Figure 1), and obtaining working curve regression equation is Y=6.31 × 10
-4x+0.0319, R
2=0.995, the range of linearity 15 μ g/mL-1mg/mL, wherein Y is lg(F
0/ F), F
0for adding the fluorescence intensity of carmine front CdTe quantum, F is the fluorescence intensity adding carmine rear CdTe quantum, and X is carmine concentration, R
2for related coefficient square.
3 examples one: 10ml is analyzed sample and boil 10 minutes removal carbon dioxide, being adjusted to pH with 1mol/L NaOH is 7.4, is finally settled to 100mL with the PBS buffer solution that pH value is 7.4; Getting 10 μ L dilutions joins in CdTe quantum solution, make the concentration of CdTe quantum identical with the concentration in above-mentioned each part standard solution, fluorescence is measured with fluorescence analyser, be 25.1 μ g/mL according to content carmine in described working curve determination analytical sample, content carmine in this analytical sample of high effective liquid chromatography for measuring is 24.9 μ g/mL, and recall rate is 100.8%.
4 examples two: get 10 μ L carmine red standard solution and join in CdTe quantum solution, make the concentration of CdTe quantum identical with the concentration in above-mentioned each part standard solution, carmine concentration is equivalent to 100.1 μ g/mL, fluorescence is measured with fluorescence analyser, be 100.2 μ g/mL according to content carmine in described working curve determination analytical sample, recall rate is 99.9%.
5 examples three: get 10 μ L carmine red standard solution and join in CdTe quantum solution, make the concentration of CdTe quantum identical with the concentration in above-mentioned each part standard solution, carmine concentration is equivalent to 990.7 μ g/mL, fluorescence is measured with fluorescence analyser, be 992.8 μ g/mL according to content carmine in described working curve determination analytical sample, recall rate is 99.8%.
6 comparative examples 1: get 10 μ L carmine red standard solution and join in CdTe quantum solution, make the concentration of CdTe quantum identical with the concentration in above-mentioned each part standard solution, carmine concentration is equivalent to 1.5mg/mL, fluorescence is measured with fluorescence analyser, be 2.1mg/mL according to content carmine in described working curve determination analytical sample, recall rate is 140%, illustrates that to exceed sensing range error too large.
7 comparative examples 2: get 10 μ L carmine red standard solution and join in CdTe quantum solution, make the concentration of CdTe quantum identical with the concentration in above-mentioned each part standard solution, carmine concentration is equivalent to 10 μ g/mL, fluorescence is measured with fluorescence analyser, be 5 μ g/mL according to content carmine in described working curve determination analytical sample, recall rate is 200%, illustrates that to exceed sensing range error too large.
Claims (5)
1. the method utilizing CdTe quantum fluorescent quenching to detect carmine content, it is characterized in that, comprise the following steps: 1) set up working curve: configure a series of concentration carmine red standard solution, wherein, carmine concentration increases gradually, add the CdTe quantum of identical amount, utilize CdTe quantum to detect the content of the famille rose in solution as fluorescence probe, draw the linear relationship between the fluorescence intensity of CdTe quantum and carmine concentration from fluorescence spectrum figure; 2) detect: analysis sample is joined in CdTe quantum solution, make the concentration of CdTe quantum identical with the concentration in a series of concentration carmine red standard solution, detect the fluorescence intensity of this analytical sample solution, according to working curve regression equation, determine content carmine in analytical sample.
2. a kind of method utilizing CdTe quantum fluorescent quenching to detect carmine content as described in 1, in above-mentioned a series of concentration carmine red standard solution, quantum dot concentration is 2 × 10
-4mol/L.
3. a kind of method utilizing CdTe quantum fluorescent quenching to detect carmine content as described in 1, carmine concentration and lg(F
0/ F) direct proportionality, wherein F
0for adding the fluorescence intensity of carmine front CdTe quantum, F is the fluorescence intensity adding carmine rear CdTe quantum.
4. a kind of method utilizing CdTe quantum fluorescent quenching to detect carmine content as described in 1, if quantum dot concentration is lower by (such as 4 × 10 in a series of concentration carmine red standard solution
-6mol/L), detection limit can reach 0.025 μ g/mL.
5. a kind of method utilizing CdTe quantum fluorescent quenching to detect carmine content as described in 1, working curve regression equation is Y=6.31 × 10
-4x+0.0319, R
2=0.995, the range of linearity 15 μ g/mL-1mg/mL, wherein Y is lg(F as described in 3
0/ F), X is carmine concentration, R
2for related coefficient square.
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Cited By (5)
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| CN105954241A (en) * | 2016-04-28 | 2016-09-21 | 吉林化工学院 | Method for detecting content of carmine in solution by utilizing graphene quantum dots |
| CN107991401A (en) * | 2017-11-01 | 2018-05-04 | 广西壮族自治区食品药品检验所 | A kind of method that 5 kinds of colouring agents synchronously detect in Crataegi pill |
| CN109613038A (en) * | 2018-12-17 | 2019-04-12 | 吉林化工学院 | A method of using fluorescent quenching quantitative analysis safranine T content |
| CN114674900A (en) * | 2022-04-02 | 2022-06-28 | 湖北大学 | Photoelectrochemical microsensor based on small molecular probe and preparation method and application thereof |
| CN114702954A (en) * | 2021-12-23 | 2022-07-05 | 郑州中科生物医学工程技术研究院 | Preparation method of fluorine-doped silicon quantum dots and application of fluorine-doped silicon quantum dots in detection of neo-carmine |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105954241A (en) * | 2016-04-28 | 2016-09-21 | 吉林化工学院 | Method for detecting content of carmine in solution by utilizing graphene quantum dots |
| CN107991401A (en) * | 2017-11-01 | 2018-05-04 | 广西壮族自治区食品药品检验所 | A kind of method that 5 kinds of colouring agents synchronously detect in Crataegi pill |
| CN107991401B (en) * | 2017-11-01 | 2021-04-20 | 广西壮族自治区食品药品检验所 | Method for synchronously detecting 5 colorants in hawthorn pills |
| CN109613038A (en) * | 2018-12-17 | 2019-04-12 | 吉林化工学院 | A method of using fluorescent quenching quantitative analysis safranine T content |
| CN114702954A (en) * | 2021-12-23 | 2022-07-05 | 郑州中科生物医学工程技术研究院 | Preparation method of fluorine-doped silicon quantum dots and application of fluorine-doped silicon quantum dots in detection of neo-carmine |
| CN114702954B (en) * | 2021-12-23 | 2023-09-05 | 郑州中科生物医学工程技术研究院 | Preparation method of fluorine-doped silicon quantum dot and application of fluorine-doped silicon quantum dot in detection of new carmine |
| CN114674900A (en) * | 2022-04-02 | 2022-06-28 | 湖北大学 | Photoelectrochemical microsensor based on small molecular probe and preparation method and application thereof |
| CN114674900B (en) * | 2022-04-02 | 2023-09-22 | 湖北大学 | Photoelectrochemical microsensor based on small molecular probe and preparation method and application thereof |
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