CN105360051A - Artificial culture seawater micro acidic electrolysis water sterilization pre-processing method - Google Patents
Artificial culture seawater micro acidic electrolysis water sterilization pre-processing method Download PDFInfo
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- CN105360051A CN105360051A CN201510794808.6A CN201510794808A CN105360051A CN 105360051 A CN105360051 A CN 105360051A CN 201510794808 A CN201510794808 A CN 201510794808A CN 105360051 A CN105360051 A CN 105360051A
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- China
- Prior art keywords
- seawater
- artificially
- brine electrolysis
- artificial
- acidic electrolysis
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- 239000013535 sea water Substances 0.000 title claims abstract description 51
- 238000005868 electrolysis reaction Methods 0.000 title claims abstract description 37
- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 20
- 230000001954 sterilising effect Effects 0.000 title claims abstract description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title abstract description 10
- 230000002378 acidificating effect Effects 0.000 title abstract description 7
- 238000000034 method Methods 0.000 title abstract description 7
- 238000007781 pre-processing Methods 0.000 title abstract 2
- 239000012267 brine Substances 0.000 claims description 31
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 claims description 31
- 230000001902 propagating effect Effects 0.000 claims description 19
- 238000002203 pretreatment Methods 0.000 claims description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- 235000002639 sodium chloride Nutrition 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- 238000010348 incorporation Methods 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 2
- 241000588724 Escherichia coli Species 0.000 abstract description 8
- 241000607272 Vibrio parahaemolyticus Species 0.000 abstract description 8
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 abstract description 6
- 239000000460 chlorine Substances 0.000 abstract description 6
- 229910052801 chlorine Inorganic materials 0.000 abstract description 6
- 238000006243 chemical reaction Methods 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 2
- 238000005259 measurement Methods 0.000 abstract 1
- 239000007788 liquid Substances 0.000 description 8
- 230000000844 anti-bacterial effect Effects 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- IOLCXVTUBQKXJR-UHFFFAOYSA-M potassium bromide Chemical compound [K+].[Br-] IOLCXVTUBQKXJR-UHFFFAOYSA-M 0.000 description 2
- PUZPDOWCWNUUKD-UHFFFAOYSA-M sodium fluoride Chemical compound [F-].[Na+] PUZPDOWCWNUUKD-UHFFFAOYSA-M 0.000 description 2
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 238000009400 out breeding Methods 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 235000013024 sodium fluoride Nutrition 0.000 description 1
- 239000011775 sodium fluoride Substances 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 229910001631 strontium chloride Inorganic materials 0.000 description 1
- AHBGXTDRMVNFER-UHFFFAOYSA-L strontium dichloride Chemical compound [Cl-].[Cl-].[Sr+2] AHBGXTDRMVNFER-UHFFFAOYSA-L 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/50—Treatment of water, waste water, or sewage by addition or application of a germicide or by oligodynamic treatment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/08—Seawater, e.g. for desalination
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Water Treatment By Electricity Or Magnetism (AREA)
Abstract
The invention discloses an artificial culture seawater micro acidic electrolysis water sterilization pre-processing method. Micro acidic electrolysis water can be added to artificial culture seawater and is mixed for 1 to 5 minutes; the volume size proportion of the micro acidic electrolysis water and the artificial culture seawater is 1 to 10 to 25. Artificial culture seawater is processed via the micro acidic electrolysis, so obvious sterilization effect is achieved; through measurements, Escherichia coli, vibrio parahaemolyticus can be detected; besides, the artificial seawater mixed with micro acidic electrolysis has low available chlorine concentration with one to threemg/L; and the artificial seawater can be restored to common water after reaction, so damage to cultured organisms can be prevented.
Description
Technical field
The present invention relates to a kind of aquaculture water sterilization technology, particularly a kind of subacidity brine electrolysis sterilization pretreatment method propagating seawater artificially.
Background technology
The effective chlorine density of subacidity brine electrolysis (Slightlyacidelectrolyzedwater, SAEW) is 10 ~ 30mg/L, pH is 5.0 ~ 6.5, and weakly acidic pH, is reduced to light water after reaction, noresidue, is a kind of green bactericide of highly effective and safe.Domestic and international researcher has found its efficient bactericidal action all, and with low cost.
Propagating seawater artificially is that artificial seawater formula (such as Schleicher and Schal artificial seawater formula etc.) conveniently carries out preparing or utilize after commercial artificial sea salt thin up, and this belongs to known technology.
Such as: the artificial seawater formula (salinity 3.34%) of Mocledon is specific as follows:
Again such as, Schleicher and Schal artificial seawater formula is specific as follows:
Solution A unit: grams per liter distilled water
Sodium chloride nacl 239.000
Magnesium chloride Mg Cl26H2O108.300
Unit water CaCl211.5
Strontium chloride SrClz6HzO0.040
Potassium chloride (KCl) 6.82
Potassium bromide KBr0.99
Solution B sodium sulphate Na2SO4lOH2O90.6
Sodium bicarbonate NaHCO30.20
Sodium fluoride NaF0.0003
Boric acid H3BO30.0027
Note: after mixing up solution A .B, sprays into B liquid in A liquid, and clarify after 24 hours, filtration can use.
What current breeding seawater sterilization mainly adopted is ozone and ultraviolet-sterilization, and these two kinds of method for disinfection all have its pros and cons.Therefore, the process for sterilizing developing a kind of novel effective and safe is necessary.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of subacidity brine electrolysis sterilization pretreatment method propagating seawater artificially, adopts method of the present invention significantly can reduce the bacteria containing amount of artificial seawater.
In order to solve the problems of the technologies described above, the invention provides a kind of subacidity brine electrolysis sterilization pretreatment method propagating seawater artificially, add subacidity brine electrolysis, incorporation time 1 ~ 5min propagating artificially in seawater;
Described subacidity brine electrolysis is 1:10 ~ 25 with the volume ratio propagating seawater artificially.
As the improvement propagating the subacidity brine electrolysis sterilization pretreatment method of seawater artificially of the present invention: according to artificial seawater formula obtain propagate seawater artificially or utilize commercial artificial sea salt preparation salinity be 3.0 ~ 4.0% propagate seawater artificially.
As the subacidity brine electrolysis sterilization pretreatment further improvements in methods propagating seawater artificially of the present invention: subacidity brine electrolysis is 1:10,1:15,1:25 with the volume ratio propagating seawater artificially.
The present invention, in experimentation, in order to verify bactericidal effect of the present invention, is propagating artificial inoculation Escherichia coli or vibrio parahemolyticus in seawater artificially.
The present invention has following beneficial effect: propagate seawater artificially through the process of subacidity brine electrolysis, and bactericidal effect is remarkable, and after measured, Escherichia coli, vibrio parahemolyticus can reach NF effect.In addition, the artificial seawater effective chlorine density after mixing subacidity brine electrolysis is low, is 1 ~ 3mg/L, and reducible be after the reaction light water, can not aquaculture organism be endangered.
Embodiment
The subacidity brine electrolysis used in following case is 30mg/L subacidity brine electrolysis (ORP is 858 ± 2mV, pH is 5.69 ± 0.04), propagates seawater artificially and forms according to the artificial seawater formulated of Mocledon.
Embodiment 1: a kind of subacidity brine electrolysis sterilization pretreatment method propagating seawater artificially, adds subacidity brine electrolysis, incorporation time 1 ~ 5min propagating artificially in seawater; Subacidity brine electrolysis is 1:10 ~ 25 with the volume ratio propagating seawater artificially.
Experiment 1,
Inoculate Escherichia coli and vibrio parahemolyticus respectively propagating artificially in seawater, make clump count reach 10
6cFU/ml, after evenly (1min) to be mixed, detect initial clump count, then (ORP is 858 ± 2mV with 30mg/L subacidity brine electrolysis, pH is 5.69 ± 0.04) at room temperature mix, make subacidity brine electrolysis be respectively 1:10,1:15,1:25 with the solid-liquid ratio containing bacterium seawater.Mix 1 respectively, 3, detect residual clump count after 5min.The sterilization result of table 1 pair Escherichia coli and vibrio parahemolyticus.
Table 1 different feed liquid is than the sterilization result of lower subacidity brine electrolysis to Escherichia coli in simulated seawater and vibrio parahemolyticus
aplate count does not detect, but Zengjing Granule result is muddy;
bplate count does not detect, and Zengjing Granule result is clarification.
Visible, when the solid-liquid ratio of subacidity brine electrolysis and seawater is greater than 1:15, through 1min, in seawater, two kinds of bacterium residual quantity are less than 1lgCFU/mL all, and through 5min, two kinds of bacterium all do not detect, and when solid-liquid ratio is increased to 1:10, in 3min, namely two kinds of bacterium reach and do not detect.Increase along with processing time and solid-liquid ratio is described, the bactericidal effect of subacidity brine electrolysis to Escherichia coli and vibrio parahemolyticus all increases, and subacidity brine electrolysis is better than Escherichia coli to the bactericidal effect of vibrio parahemolyticus in seawater.
In addition there are some researches prove, chlorine residual concentration lower than during 2mg/L to cultivation fry without harm.When solid-liquid ratio is 1:15, cl concn is about 2mg/L, and the rear chlorine residual concentration of reaction is lower than 2mg/L, and when brine electrolysis exposes in atmosphere, available chlorine has certain degraded, belongs to safe range.In view of electrolytic seawater has corrosivity, subacidity brine electrolysis can be carried out breeding seawater sterilization as bactericidal additive.
Finally, it is also to be noted that what enumerate above is only several specific embodiments of the present invention.Obviously, the invention is not restricted to above embodiment, many distortion can also be had.All distortion that those of ordinary skill in the art can directly derive from content disclosed by the invention or associate, all should think protection scope of the present invention.
Claims (3)
1. propagate the subacidity brine electrolysis sterilization pretreatment method of seawater artificially, it is characterized in that: add subacidity brine electrolysis, incorporation time 1 ~ 5min propagating artificially in seawater;
Described subacidity brine electrolysis is 1:10 ~ 25 with the volume ratio propagating seawater artificially.
2. the subacidity brine electrolysis sterilization pretreatment method propagating seawater artificially according to claim 1, is characterized in that:
According to artificial seawater formula obtain propagate seawater artificially or utilize commercial artificial sea salt preparation salinity be 3.0 ~ 4.0% propagate seawater artificially.
3. the subacidity brine electrolysis sterilization pretreatment method propagating seawater artificially according to claim 1 and 2, is characterized in that: described subacidity brine electrolysis is 1:10,1:15,1:25 with the volume ratio propagating seawater artificially.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510794808.6A CN105360051A (en) | 2015-11-18 | 2015-11-18 | Artificial culture seawater micro acidic electrolysis water sterilization pre-processing method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510794808.6A CN105360051A (en) | 2015-11-18 | 2015-11-18 | Artificial culture seawater micro acidic electrolysis water sterilization pre-processing method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105360051A true CN105360051A (en) | 2016-03-02 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510794808.6A Pending CN105360051A (en) | 2015-11-18 | 2015-11-18 | Artificial culture seawater micro acidic electrolysis water sterilization pre-processing method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105360051A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110089482A (en) * | 2019-05-21 | 2019-08-06 | 浙江大学 | A kind of pond circulation water purification of water quality plug-flow oxygen-increasing device and setting method based on electrolysis water |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101223885A (en) * | 2008-02-01 | 2008-07-23 | 中国农业大学 | Micro electrolysis disinfecting preparation and preparing method thereof |
| CN102838242A (en) * | 2011-06-21 | 2012-12-26 | 株式会社神户制钢所 | Sterilization method of seawater, sterilization composition generation device |
-
2015
- 2015-11-18 CN CN201510794808.6A patent/CN105360051A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101223885A (en) * | 2008-02-01 | 2008-07-23 | 中国农业大学 | Micro electrolysis disinfecting preparation and preparing method thereof |
| CN102838242A (en) * | 2011-06-21 | 2012-12-26 | 株式会社神户制钢所 | Sterilization method of seawater, sterilization composition generation device |
Non-Patent Citations (4)
| Title |
|---|
| 王春芳: "海水养殖水的电解水杀菌研究", 《浙江大学硕士学位论文》 * |
| 祁凡雨: "混比条件下微酸性电解水对养殖水体的消毒动力学研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
| 罗日祥等: "电解海水制氯消毒处理对虾培苗海水的研究", 《海洋科学》 * |
| 黄钰茹: "电解氧化水处理水产品之杀菌效果及安全性之探讨", 《国立台湾大学博士学位论文》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110089482A (en) * | 2019-05-21 | 2019-08-06 | 浙江大学 | A kind of pond circulation water purification of water quality plug-flow oxygen-increasing device and setting method based on electrolysis water |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160302 |