CN105353514B - Reduce method, the method and apparatus of laser microprobe dating of laser beam cross-section product - Google Patents
Reduce method, the method and apparatus of laser microprobe dating of laser beam cross-section product Download PDFInfo
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- CN105353514B CN105353514B CN201510783316.7A CN201510783316A CN105353514B CN 105353514 B CN105353514 B CN 105353514B CN 201510783316 A CN201510783316 A CN 201510783316A CN 105353514 B CN105353514 B CN 105353514B
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- G—PHYSICS
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- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/09—Beam shaping, e.g. changing the cross-sectional area, not otherwise provided for
- G02B27/0933—Systems for active beam shaping by rapid movement of an element
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- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
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- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/09—Beam shaping, e.g. changing the cross-sectional area, not otherwise provided for
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Abstract
The present invention provides a kind of method of reduction laser beam cross-section product, installation space optical modulator in the light path between laser emitter and illuminated object；The incoming laser beam that the laser emitter is sent out generates main spot and secondary hot spot in spatial light modulator；The main spot covers on N number of adjacent micro unit of spatial light modulator, only open the n micro unit (n=1 centered on the main spot center of circle, 2, ..., N), other N n micro units are in off state, then the cross-sectional area d=D* (n/N) of shoot laser beam, and wherein D is the cross-sectional area of main spot.The present invention also provides a kind of method and apparatus of laser microprobe dating.A kind of method of above-mentioned reduction laser beam cross-section product makes the cross-sectional area of laser beam reduce in a certain range by manual operation and control.A kind of method and apparatus of above-mentioned laser microprobe dating can realize that cutting line width is small to the micron even laser cutting of sub-micron.
Description
Technical field
The present invention relates to laser cutting field more particularly to laser microprobe datings.
Background technology
In life science, need to carry out gene and protein research to a certain specific cell (group) in tissue.
Laser capture microdissection cell cutting system is exactly a kind of high-end devices to need to grow up as satisfaction, can be from diversity
Tissue in separate cell (group) that is a certain specific or having a certain feature, to avoid experimental specimen by other cells,
Bacterium or other impurity are polluted, and keep the analysis of gene and albumen more accurate, specific higher.
In laser capture microdissection cell cutting technology, it is sometimes desirable to be cut to individual cells：It sees under the microscope first
It examines judgement and determines and need select cell；Next is determined according to cell shape after cell cutting line；Emit laser later
Beam, driving objective table makes it that the glass slide movement with cell sample be driven to generate closing of cutting line while emitting laser beam
Track.Laser just generates a closing of cutting track along the cutting line on cell periphery in this way so that the cell and ambient background
Other cells separation.The cell selection can be isolated by modes such as collection system such as gravity or Electrostatic Absorption later
Come, to obtain the cell of purebred not impurity.
The scale of cell is very small between a micron more than several microns to 10, it is sometimes necessary to cutting separation user
One section of gene of (be included in cell inside) selected, gene it is smaller.Therefore it needs " knife " of incising cell：
The hot spot cross-sectional area of laser beam is very small, in sub-micron or micron dimension, separation cutting user is especially needed to be selected
Cell contained by genetic fragment when.General laser beam spot cross section is approximately garden or ellipse, along one
The scale in direction is 100 microns of even millimeter magnitudes.Therefore how to realize that the length of cross section is swashing for micron even sub-micron
Light beam is a problem.
Invention content
Technical problem underlying to be solved by this invention is to provide a kind of method of reduction laser beam cross-section product, passes through people
To operate and controlling, the cross-sectional area of laser beam is made to reduce in a certain range.
Another technical problem underlying to be solved by this invention is to provide a kind of method and apparatus of laser microprobe dating, energy
Enough micron even laser cuttings of sub-micron diameter realized.
In order to solve the above technical problems, the present invention provides a kind of method of reduction laser beam cross-section product, swashing
Installation space optical modulator in light path between optical transmitting set and illuminated object；The incoming laser beam that the laser emitter is sent out
Main spot and secondary hot spot are generated in spatial light modulator；
The main spot covers on N number of adjacent micro unit of spatial light modulator, during only opening is with the main spot center of circle
N micro unit of the heart (n=1,2 ..., N), other N-n micro units are in off state, then the cross-sectional area d=of shoot laser beam
D* (n/N), wherein D are the cross-sectional area of main spot.
In a preferred embodiment：The micro unit for the spatial light modulator that the pair hot spot covers is in off state.
The present invention also provides a kind of methods of laser microprobe dating, are arranged in the light path of laser emitter and object lens empty
Between optical modulator；The incoming laser beam that the laser emitter is sent out generates main spot and secondary hot spot in spatial light modulator；
The main spot covers on N number of adjacent micro unit of spatial light modulator, during only opening is with the main spot center of circle
N micro unit of the heart (n=1,2 ..., N), other N-n micro units are in off state, then the shoot laser beam after object lens
Cross-sectional area d=D*n/ (N*M), wherein D are the cross-sectional area of main spot, the amplification factor that M is object lens.
In a preferred embodiment：The micro unit for the spatial light modulator that the pair hot spot covers is in off state.
The present invention also provides a kind of device of laser microprobe dating, including microscope, microscope illumination system, number at
As system and laser emitter；Further include laser beam optical path orthopedic systems comprising one is set to laser emitter and microscope
Spatial light modulator in object lens light path；The incoming laser beam that the laser emitter is sent out generates master in spatial light modulator
Hot spot and secondary hot spot；
The main spot covers on N number of adjacent micro unit of spatial light modulator, during only opening is with the main spot center of circle
N micro unit of the heart (n=1,2 ..., N), other N-n micro units are in off state, then the shoot laser beam after object lens
Cross-sectional area d=D*n/ (N*M), wherein D are the cross-sectional area of main spot, the amplification factor that M is object lens.
In a preferred embodiment：The micro unit for the spatial light modulator that the pair hot spot covers is in off state.
In a preferred embodiment：The laser beam optical path orthopedic systems further include a dichroscope, anti-by laser is cut
It is incident upon object lens, image-forming objective lens is transmitted through digital imaging system.
In a preferred embodiment：The microscope is just to set microscope or inverted microscope
Description of the drawings
Fig. 1 is the index path of the preferred embodiment of the present invention 1；.
Fig. 2 is the light path schematic diagram of the preferred embodiment of the present invention 2；
Fig. 3 is the light path schematic diagram of the preferred embodiment of the present invention 3；
Fig. 4 is the light path schematic diagram of the preferred embodiment of the present invention 4.
Specific implementation mode
The length of present invention realization cross-sectional area is use space light for the method for sub-micron or micron size laser beam
Modulator.Spatial light modulator mainly has transmission-type and reflective two class, is mainly characterized by with " the light with spatial distribution
Switch ".Major function be can selectively allow be radiated at spatial light modulator portion optical signal reflection (or thoroughly
Penetrate) past, and the optical signal of other parts position cannot reflect (or transmissive).
Using DMD as the introduction of spatial light modulator：DMD for TI companies Larry doctors Hornbeck in institute in 1987
Invention, micro reflector array and CMOS SRAM are integrated on same chip using the technique of MEMS (MEMS),
It is a kind of novel, totally digitilized spatial light modulator.DMD is made of millions of a micro unit units, and one contains
1024X768 only 0.7 inch of micro unit DMD catercorner length, about 5 millimeters of thickness (as shown in Figure 1).Each micro unit unit
Mainly it is made of micro unit, hinge and CMOS substrates, wherein each micro unit is connected by a yoke plate and a torsion hinge,
Can be rotated between -12 ° and+12 ° the two angles using hinge as axis, rotation direction by yoke plate both sides controllable electrodes voltage
It determines, there are one storage unit SRAM for the electrode of each micro unit, are located under the platform of controllable electrodes, when SRAM is stored
When " 1 ", micro unit deflects+12 °, when storage " 0 ", deflects -12 °.When designing the optical system of DMD, generally make micro unit
The light of some reflection direction enter optical system, indicate "ON" (being assumed to be+12 ° of directions, similar below), another reflection
The light in direction will not enter optical system, indicate "Off".Thus can DMD simply be regarded as an array of photoswitch,
" 1 " or " 0 " is stored in corresponding sram cell, so that it may arbitrary to generate to control the switch of corresponding micro unit as needed
Pattern.
Although it should be pointed out that illustrated above with DMD, the solution of the present invention is not limited to a kind of this sky of DMD
Between in optical modulator.Below in conjunction with the drawings and specific embodiments, the present invention will be further described.
Embodiment 1
A method of reducing laser beam cross-section product, is arranged in the light path between laser emitter and illuminated object empty
Between optical modulator；The incoming laser beam that the laser emitter is sent out generates main spot and secondary hot spot in spatial light modulator；
With reference to figure 1, the main spot covers on N number of adjacent micro unit of spatial light modulator, only opens with key light
N micro unit (n=1,2 ..., N), other N-n micro units are in off state centered on the spot center of circle, then the cross of shoot laser beam
Sectional area d=D* (n/N), wherein D are the cross-sectional area of main spot.To reach the cross-sectional area for reducing shoot laser beam,
Also, changes the quantity for closing micro unit, the cross-sectional area of outgoing laser beam can be made to change in a certain range.To realize
The simple purpose for changing laser beam cross-section product.
Further, the micro unit for the spatial light modulator that the secondary hot spot covers is in off state.Light secondary in this way
The shoot laser of spot part is just all separated with the shoot laser of main spot, so that secondary hot spot is eliminated.
Embodiment 2
With reference to figure 2, a kind of device of laser microprobe dating, including just setting microscope, microscope illumination system, digital imagery
System and laser emitter 1；Further include laser beam optical path orthopedic systems comprising one is set to laser emitter 1 and microscope
Spatial light modulator 3 in 2 light path of object lens；The incoming laser beam that the laser emitter 1 is sent out produces in spatial light modulator 3
Raw main spot and secondary hot spot；
The main spot covers on N number of adjacent micro unit of spatial light modulator, and only opening is with the main spot center of circle
N, center micro unit (n=1,2 ..., N), other N-n micro units are in off state, then the shoot laser beam after object lens
Cross-sectional area d=D*n/ (N*M), wherein D is the cross-sectional area of main spot, the amplification factor that M is object lens.
Assuming that the main spot cross-sectional area D=100um*100um for the laser beam cross-section product that laser emitter 1 generates, object
M=100 times of the amplification factor of mirror 2.And the length of side of micro unit is in 10 microns.Therefore the micro unit number of this main spot covering
N=10*10.The cross-sectional area d=0.1 for the minimum shoot laser beam that the device of so above-mentioned laser microprobe dating is realized is micro-
* 0.1 micron of rice only opens main spot circle centre position and obtains a micro unit, makes n=1.That is the width of cutting line reaches 0.1 micron.
In view of diffraction effect, the spot size on cell sample is beaten also in pattern of sub-micron level, can be met unicellular or even sub- thin
The needs of born of the same parents' cutting.
Further, the micro unit for the spatial light modulator 3 that the secondary hot spot covers is in off state.Light secondary in this way
The shoot laser of spot part is just all separated with the shoot laser of main spot, so that secondary hot spot is eliminated.
Further, the laser beam optical path orthopedic systems further include a dichroscope 5, by cutting laser reflection to object
Image-forming objective lens are transmitted through digital imaging system by mirror.
In the present embodiment, the optical system of lighting source includes condenser 6 and lighting source 7；What lighting source 7 was sent out
Light exposes to motorized subject table 8 after condenser 6, and side of the motorized subject table 8 far from lighting source is placed with cutting mesh
Mark object 9.And the shoot laser beam direct irradiation sent out by object lens 2 is on cutting object 9.
Embodiment 3
The present embodiment the difference from example 2 is that, be changed to inverted microscope by microscope is just set.With reference to figure 3, only need
Cutting object 9 is placed on motorized subject table 8 towards the side of epi-illumination light source 7.And sent out by object lens 2
Shoot laser beam is radiated at across motorized subject table 8 on cutting object 9.
Embodiment 4
Difference lies in replace with light source for transmission illumination 7, reference chart to the present embodiment by epi-illumination light source with embodiment 3
4, the light that light source for transmission illumination 7 is sent out exposes to motorized subject table 8 after 6, and motorized subject table 8 is far from light source for transmission illumination
7 side is placed with cutting object 9.And cutting is radiated at across motorized subject table 8 by the shoot laser beam that object lens 2 are sent out
On object 9.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Any one skilled in the art in the technical scope disclosed by the present invention, the change or replacement that can be readily occurred in,
It should be covered by the protection scope of the present invention.Therefore, protection scope of the present invention should be with scope of the claims
Subject to.
Claims (5)
1. a kind of method reducing laser beam cross-section product, it is characterised in that：Light between laser emitter and illuminated object
Installation space optical modulator in road；The incoming laser beam that the laser emitter is sent out generates main spot in spatial light modulator
With secondary hot spot；
The main spot covers on N number of adjacent micro unit of spatial light modulator, only opens the n centered on the main spot center of circle
Micro unit, n=1,2 ..., N, other N-n micro units are in off state, and the micro unit covered by secondary hot spot is to close shape
State, then the cross-sectional area d=D* (n/N) of shoot laser beam, wherein D are the cross-sectional area of main spot.
2. a kind of method of laser microprobe dating, it is characterised in that：The installation space light in the light path of laser emitter and object lens
Modulator；The incoming laser beam that the laser emitter is sent out generates main spot and secondary hot spot in spatial light modulator；
The main spot covers on N number of adjacent micro unit of spatial light modulator, only opens the n centered on the main spot center of circle
Micro unit, n=1,2 ..., N, other N-n micro units are in off state, and the micro unit covered by secondary hot spot is to close shape
State.
3. a kind of device of laser microprobe dating, including microscope, microscope illumination system, digital imaging system and Laser emission
Device, it is characterised in that further include laser beam optical path orthopedic systems comprising one is set to laser emitter and micro objective light
Spatial light modulator in road；The incoming laser beam that the laser emitter is sent out generated in spatial light modulator main spot and
Secondary hot spot；
The main spot covers on N number of adjacent micro unit of spatial light modulator, only opens the n centered on the main spot center of circle
Micro unit, n=1,2 ..., N, other N-n micro units are in off state, and the micro unit covered by secondary hot spot is to close shape
State.
4. a kind of device of laser microprobe dating according to claim 3, it is characterised in that：The laser beam optical path shaping
System further includes a dichroscope, will cutting laser reflection to object lens, number will be transmitted through by the image optics signal of object lens
Imaging system.
5. a kind of device of laser microprobe dating according to any one of claim 3-4, it is characterised in that：It is described micro-
Mirror is just to set microscope or inverted microscope.
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| CN201510783316.7A CN105353514B (en) | 2015-11-16 | 2015-11-16 | Reduce method, the method and apparatus of laser microprobe dating of laser beam cross-section product |
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| US7807108B2 (en) * | 2006-09-18 | 2010-10-05 | Leica Microsystems Cms Gmbh | Apparatus for receiving biological specimens |
| JP4711155B2 (en) * | 2009-06-30 | 2011-06-29 | カシオ計算機株式会社 | Light source device and projector |
| DE102013200367A1 (en) * | 2013-01-14 | 2014-01-23 | Carl Zeiss Smt Gmbh | Laser divergence manipulator for laser light source for projection exposure system for use in microlithography, has beam moving unit for local variation of laser beam relative to target area, so that beam cross-section is displaced |
| CN103197413B (en) * | 2013-04-15 | 2017-03-15 | 厦门大学 | The optical microscope imaging method of three-dimensional image can be produced |
| US9658447B2 (en) * | 2013-12-09 | 2017-05-23 | Texas Instruments Incorporated | Multiple illumination sources for DMD lighting apparatus and methods |
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