CN105353153A - Gene detection microfluidic chip system - Google Patents
Gene detection microfluidic chip system Download PDFInfo
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- CN105353153A CN105353153A CN201510785104.2A CN201510785104A CN105353153A CN 105353153 A CN105353153 A CN 105353153A CN 201510785104 A CN201510785104 A CN 201510785104A CN 105353153 A CN105353153 A CN 105353153A
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Abstract
The invention relates to a gene detection microfluidic chip system. The system is used in the technical field of gene detection. The system comprises a microfluidic chip set, a DNA detection analysis plate arranged below the microfluidic chip set, a signal processor and a computer control terminal. The microfluidic chip set comprises cover glass, a PCR amplification plate and a thermal-insulation insulating base. The microfluidic chip set is connected to the DNA detection analysis plate by a DNA detection data line. The DNA detection analysis plate is connected to the signal processor. The signal processor is connected to the computer control terminal. The microfluidic chip set and the detection system can realize detection of DNA, reduce the number of equipment and reduce a purchase cost. The system has a high automation degree and high work efficiency. The microfluidic chip sets can run in parallel so that sample and reagent consumption is reduced, an operation cost is reduced and a sample pollution risk is reduced. The system has a small volume and can form a portable instrument and satisfy real-time on-site detection requirements.
Description
Technical field
The invention belongs to technical field of gene detection, particularly relate to a kind of micro-fluidic chip system for genetic test.
Background technology
PCR is a kind of Protocols in Molecular Biology for amplifying the specific DNA fragmentation that increases, and it can regard the special DNA replication dna of in vitro as, is the maximum feature of PCR, the DNA of trace significantly can be increased.Therefore, be no matter the remains of the extinct plants and animal in fossil, historical personage, hair, skin or blood that still assailant leaves in murder case decades ago, as long as can isolate the DNA of a wee bit, just can be amplified with PCR, be compared.This is also the place of the power of " micro-evidence ".First advanced an idea by nineteen eighty-three U.S. Mullis, within 1985, invented polymerase chain reaction by it, i.e. simple DNA TRAP, mean the real birth of round pcr.2013 by now, PCR developed into third-generation technology.1973, Taiwanese scientist Qian Jiayun, found stable Taq DNA polymerase, for basic contribution has also been made in round pcr development.
Gene magnification and genetic test integrated instrument are commercially comparatively common is quantitative real time PCR Instrument, in gene amplification process, carry out real-time fluoroscopic examination.But this detection can only be assessed the concentration of gene, and the specifying information such as length and sequence of gene cannot be known.Length, the concentration of gene detect by the mode of electrophoresis, and the sequence of gene also can be detected by the mode of Capillary Electrophoresis.
But up to the present, market does not also occur gene magnification and electrophoretic genetic test become one by one, realize the system of robotization, be a lot in the technology of development, there is a lot of technology barriers yet.
Summary of the invention
For solving the problems of the technologies described above, the object of the invention is to provide a kind of micro-fluidic chip system for genetic test.
The solution of the present invention is as follows:
For a micro-fluidic chip system for genetic test, comprise micro-fluidic chip group, be arranged on DNA below micro-fluidic chip group and detect analysis plates, signal processor and computing machine control terminal;
Described micro-fluidic chip group comprises cover glass, pcr amplification plate and partiting thermal insulation base; Described pcr amplification plate is provided with the sample preparation pond of multi-set parallel arrangement; Sample waste pond, buffer pool, sample cell and waste liquid pool is provided with in described sample preparation pond; Sample waste pond is connected by PCR sample intake passage with sample cell; Buffer pool is connected by CE split tunnel with waste liquid pool; Multiple array electrode is evenly provided with in PCR sample intake passage and CE split tunnel;
Described array electrode is connected with electrode controller; Described micro-fluidic chip group detects data line by DNA and is connected with DNA detection analysis plates; Described DNA detects analysis plates and is connected with signal processor; Described signal processor is connected with computing machine control terminal;
Described electrode controller comprises central processing unit, optocoupler driver element, digital to analog converter and voltage module; ADC unit and DMA unit is integrated with in described central processing unit; Described optocoupler driver element, digital to analog converter are connected with central processing unit respectively; Signal through digital to analog converter process again by being connected with voltage module after impact damper process; Voltage module by after amplifilter again signal transmission in central processing unit; Described optocoupler driver element is also connected with voltage module, implements the control of pair array electrode.
The CE split tunnel in described sample preparation pond is helical structure, and CE split tunnel one end is connected with waste liquid pool, and the other end is connected with buffer pool.
The CE split tunnel in described sample preparation pond and PCR sample intake passage across structure, CE split tunnel one end is connected with waste liquid pool, and the other end is connected with buffer pool.
Described central processing unit is also connected with matrix keyboard control module and LCD screen display unit.
The electrode base sheet of described matrix electrodes adopts platinum material.
Described electrode base sheet is coated with silicon dioxide insulating layer, and the thickness of silicon dioxide insulating layer is 2.5-3.5 micron.
Described cover glass lower surface is provided with PDMS dielectric film.
Native system tool has the following advantages and beneficial effect: use the detection that PCR and CE integrated microfluidic chip group and detection system thereof are carried out DNA gene, decrease the quantity of equipment, reduce hardware acquisition cost; Improve the automaticity of analytic process, increase work efficiency; Avoid sample transfer between different devices, simplify manual operation, reduce the risk of sample contamination; Micro-fluidic chip group can parallel running, decreases the consumption of sample and reagent, reduces operating cost; System and device volume of the present invention is little, can form portable set, meets the detection needs of real time implementation, scene, has very large marketing prospect.
Accompanying drawing explanation
Fig. 1 is present system structural representation;
Fig. 2 is micro-fluidic chip group structural representation of the present invention;
Fig. 3 is the sample preparation pool structure schematic diagram on pcr amplification plate of the present invention;
Fig. 4 is the another kind of structural representation of CE split tunnel on pcr amplification plate of the present invention;
Fig. 5 is that electrode controller of the present invention controls schematic diagram.
Wherein: 1, computing machine control terminal; 2, electrode controller; 3, DNA detects data line; 4, micro-fluidic chip group; 5, DNA detects analysis plates; 6, signal processor; 41, sample preparation pond; 42, cover glass; 43, pcr amplification plate; 44, partiting thermal insulation base; 411, sample waste pond; 412, PCR sample intake passage; 413, buffer pool; 414, sample cell; 415, CE split tunnel; 416, waste liquid pool; 417, array electrode.
Embodiment
Below in conjunction with accompanying drawing, the present invention will be further described;
As shown in Figures 1 to 5, a kind of micro-fluidic chip system for genetic test, comprises micro-fluidic chip group 4, is arranged on DNA below micro-fluidic chip group 4 and detects analysis plates 5, signal processor 2 and computing machine control terminal 1;
Described micro-fluidic chip group 4 comprises cover glass 42, pcr amplification plate 43 and partiting thermal insulation base 44; Described pcr amplification plate 43 is provided with the sample preparation pond 41 of multi-set parallel arrangement, we can select the quantity in the sample preparation pond 41 arranged to reach the object of efficient process as required, can arrange 300 groups at most at present; Sample waste pond 411, buffer pool 413, sample cell 414 and waste liquid pool 416 is provided with in described sample preparation pond 41; Sample waste pond 411 is connected by PCR sample intake passage 412 with sample cell 414; Buffer pool 413 is connected by CE split tunnel 415 with waste liquid pool 416; Multiple array electrode 417 is evenly provided with in PCR sample intake passage 412 and CE split tunnel 415; By adding the appropriate DNA reagent containing sample in sample cell 414, being increased by PCR sample intake passage 412, being separated from CE split tunnel 415.In order to improve the effect of amplification, temperature control module can be increased in micro-fluidic chip group 4, promoting the amplification of DNA gene.
Described array electrode 417 is connected with electrode controller 2; Described micro-fluidic chip group 4 detects data line 3 by DNA and is connected with DNA detection analysis plates 5, detects gene detect the DNA of amplification; Described DNA detects analysis plates 5 and is connected with signal processor 2; Described signal processor 2 is connected with computing machine control terminal 1; Computing machine control terminal 1 can be communicated information to, and by being arranged on matrix keyboard control module on central processing unit and LCD screen display unit, realize Automated condtrol and display, more convenient, quick, visual good, greatly can reduce labour intensity.
Described electrode controller 2 comprises central processing unit, optocoupler driver element, digital to analog converter and voltage module; ADC unit and DMA unit is integrated with in described central processing unit; Described optocoupler driver element, digital to analog converter are connected with central processing unit respectively; Signal through digital to analog converter process again by being connected with voltage module after impact damper process; Voltage module by after amplifilter again signal transmission in central processing unit; Described optocoupler driver element is also connected with voltage module, implements the control of pair array electrode.
The CE split tunnel 415 in described sample preparation pond 41 can be set to helical structure, and CE split tunnel 415 one end is connected with waste liquid pool 416, and the other end is connected with buffer pool 413.The CE split tunnel 415 in described sample preparation pond 41 can be arranged to and PCR sample intake passage 412 across structure, and CE split tunnel 415 one end is connected with waste liquid pool 416, and the other end is connected with buffer pool 413.Wherein helical structure can extend CE split tunnel 415, does not increase again the area of micro-fluidic chip group, is a kind of better selection.And above-mentioned two kinds of structures can reduce the leakage of sample separation.
As preferential, the electrode base sheet of described matrix electrodes adopts platinum material.
As preferential, described electrode base sheet is coated with silicon dioxide insulating layer, the thickness of silicon dioxide insulating layer is 2.5-3.5 micron, effectively can eliminate the bubble in electrode process.
As preferential, described cover glass 42 lower surface is provided with PDMS dielectric film, and the preferential thickness of PDMS dielectric film is 4-4.5 micron, and voltage endurance capability can significantly improve, and meets the demand of voltage electric field.And the processing of PDMS dielectric film is simple, the chip of making is easy to encapsulation, and nontoxic, light transmission is good.
Although give detailed description and explanation to the specific embodiment of the present invention above; but what should indicate is; we can carry out various equivalence according to conception of the present invention to above-mentioned embodiment and change and amendment; its function produced do not exceed that instructions contains yet spiritual time, all should within protection scope of the present invention.
Claims (7)
1., for a micro-fluidic chip system for genetic test, comprise micro-fluidic chip group, be arranged on DNA below micro-fluidic chip group and detect analysis plates, signal processor and computing machine control terminal;
Described micro-fluidic chip group comprises cover glass, pcr amplification plate and partiting thermal insulation base; Described pcr amplification plate is provided with the sample preparation pond of multi-set parallel arrangement; Sample waste pond, buffer pool, sample cell and waste liquid pool is provided with in described sample preparation pond; Sample waste pond is connected by PCR sample intake passage with sample cell; Buffer pool is connected by CE split tunnel with waste liquid pool; Multiple array electrode is evenly provided with in PCR sample intake passage and CE split tunnel;
Described array electrode is connected with electrode controller; Described micro-fluidic chip group detects data line by DNA and is connected with DNA detection analysis plates; Described DNA detects analysis plates and is connected with signal processor; Described signal processor is connected with computing machine control terminal;
Described electrode controller comprises central processing unit, optocoupler driver element, digital to analog converter and voltage module; ADC unit and DMA unit is integrated with in described central processing unit; Described optocoupler driver element, digital to analog converter are connected with central processing unit respectively; Signal through digital to analog converter process again by being connected with voltage module after impact damper process; Voltage module by after amplifilter again signal transmission in central processing unit; Described optocoupler driver element is also connected with voltage module, implements the control of pair array electrode.
2. a kind of micro-fluidic chip system for genetic test according to claim 1, is characterized in that: the CE split tunnel in described sample preparation pond is helical structure, and CE split tunnel one end is connected with waste liquid pool, and the other end is connected with buffer pool.
3. a kind of micro-fluidic chip system for genetic test according to claim 1, it is characterized in that: the CE split tunnel in described sample preparation pond and PCR sample intake passage across structure, CE split tunnel one end is connected with waste liquid pool, and the other end is connected with buffer pool.
4. a kind of micro-fluidic chip system for genetic test according to claim 1, is characterized in that: described central processing unit is also connected with matrix keyboard control module and LCD screen display unit.
5. a kind of micro-fluidic chip system for genetic test according to claim 1, is characterized in that: the electrode base sheet of described matrix electrodes adopts platinum material.
6. a kind of micro-fluidic chip system for genetic test according to claim 5, is characterized in that: described electrode base sheet is coated with silicon dioxide insulating layer, and the thickness of silicon dioxide insulating layer is 2.5-3.5 micron.
7. a kind of micro-fluidic chip system for genetic test according to claim 1, is characterized in that: described cover glass lower surface is provided with PDMS dielectric film.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510785104.2A CN105353153A (en) | 2015-11-17 | 2015-11-17 | Gene detection microfluidic chip system |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510785104.2A CN105353153A (en) | 2015-11-17 | 2015-11-17 | Gene detection microfluidic chip system |
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| CN105353153A true CN105353153A (en) | 2016-02-24 |
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| CN201510785104.2A Pending CN105353153A (en) | 2015-11-17 | 2015-11-17 | Gene detection microfluidic chip system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107153122A (en) * | 2017-05-27 | 2017-09-12 | 哈尔滨工业大学（威海） | Digital microcurrent-controlled biochip in situ rest structure and method based on serial communication |
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- 2015-11-17 CN CN201510785104.2A patent/CN105353153A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107153122A (en) * | 2017-05-27 | 2017-09-12 | 哈尔滨工业大学（威海） | Digital microcurrent-controlled biochip in situ rest structure and method based on serial communication |
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Application publication date: 20160224 |
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