CN105352774B - A kind of full-automatic tissue paraffin section de-waxing reaction box - Google Patents
A kind of full-automatic tissue paraffin section de-waxing reaction box Download PDFInfo
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- CN105352774B CN105352774B CN201510920129.9A CN201510920129A CN105352774B CN 105352774 B CN105352774 B CN 105352774B CN 201510920129 A CN201510920129 A CN 201510920129A CN 105352774 B CN105352774 B CN 105352774B
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Abstract
The invention discloses the present invention relates to a kind of full-automatic tissue paraffin section de-waxing reaction box, including reagent circulation passage, waste liquid tank, reaction cavity, reagent injection channel；Reagent circulation passage one end is connected with inlet, is also interconnected with each reagent injection channel, and mouth is placed in reaction cavity top for sample, and bottom and reagent injection channel are interconnected, and react the waste liquid tank being surrounded with the top of box body.The configuration of the present invention is simple, can allow reagent to wash away tissue paraffin section de, allow reagent and paraffin fully to react, and the paraffin taken off on tissue paraffin section de can be completely exhausted out from reaction cavity top, make dewaxing more complete.Suitable for the batch dewaxing treatment of tissue paraffin section de.
Description
Technical field
A kind of pathological tissue microtomy technology of the present invention, and in particular to full-automatic tissue paraffin section de-waxing reaction
Box, suitable for the dewaxing treatment of various paraffin tissue sections.
Background technology
Tissue paraffin section de is most common a kind of section skill in the qualitative analysis such as tissues observed form and immunohistochemistry
Art, is widely used in Clinicopathologic Diagnosis and experimental study.Immunohistochemistry is carried out for tissues observed and to tissue, is first had to
Except the paraffin of paraffin section, i.e., de- paraffin.The conventional process for dewaxing of tissue paraffin section de is that section is sequentially placed into equipped with difference
The reaction box of reagent carries out dewaxing treatment.It is not only cumbersome, it is necessary to multiple reaction cavities in the process for dewaxing, but also need
There is special messenger to be operated, operation order mistake easily occur.But to improve such case, up to the present, the dewaxing of improvement
Method has to be replaced by mechanical arm movement section to differential responses box, or by being vacuumized to cavity to complete reagent, but
There are cost of manufacture it is high the shortcomings that.
The content of the invention
The purpose of the present invention is be in view of the above-mentioned problems existing in the prior art, there is provided a kind of full-automatic tissue paraffin is cut
Piece dewaxing reaction box.Make histotomy dewaxing treatment only need a reaction box to complete, saved reaction box, reduce operation
Complexity, make the histotomy after dewaxing cleaner.
A kind of full-automatic tissue paraffin section de-waxing reaction box, including reaction box body, the bottom of the reaction box body is set
Reagent circulation passage is equipped with, the side of reaction box body bottom, which is provided with, connects inlet with reagent circulation passage, in reaction box body
The reaction cavity being provided with above the reagent circulation passage, the top surface opening of reaction cavity, reacts the top ring winding of box body
The waste liquid tank being equipped with, the bottom of waste liquid tank are provided with liquid outlet, and the fixing groove for fixed section, examination are provided with reaction cavity
Connected between agent circulation passage and reaction cavity by reagent injection channel.
Inlet and liquid outlet as described above are positioned at the opposite both sides of reaction box body.
Section as described above is multiple, direction of each section along inlet side to the liquid outlet side of reaction box body
It is sequentially distributed in reaction cavity, the number of reagent injection channel is corresponding with the number cut into slices, from the outgoing of reagent injection channel
Reagent is injected in corresponding section bottom.
Direction extension of the reagent circulation passage as described above along inlet side to the liquid outlet side of reaction box body, and
The cross section of reagent circulation passage is sequentially reduced along the direction of inlet side to the liquid outlet side of reaction box body.
Side of the cross section of reagent injection channel as described above along inlet side to the liquid outlet side of reaction box body
To being sequentially reduced.
The cross section of reagent injection channel as described above is arc.
The top surface of reaction cavity as described above is higher than the top surface of waste liquid tank.
Compared with prior art, the present invention haing the following advantages and effect：
1st, histotomy is washed away by special construction, makes dewaxing more complete, more totally.
2nd, the wax of floating can flow into waste liquid tank from the top of reaction box, avoid the attachment again of wax stone.
3rd, reaction box quantity is reduced, it is cost-effective.
4th, frequent switching reaction box is not required in histotomy dewaxing reaction, reduces the pollution to section.
Brief description of the drawings
Fig. 1 is the side sectional view of the present invention.
In figure：1- inlets, 2- reaction box bodys, 3- waste liquid tanks, 4- reaction cavities；5- fixing grooves；6- liquid outlets；7- is cut
Piece；8- reagent injection channels；9- reagent circulation passages.
Embodiment
Invention is further described with reference to embodiment and attached drawing.
A kind of full-automatic tissue paraffin section de-waxing reaction box, including reaction box body 2, the bottom of the reaction box body 2
Reagent circulation passage 9 is provided with, the side of 2 bottom of reaction box body is provided with connects inlet 1 with reagent circulation passage 9, reacts
The reaction cavity 4 being provided with box body 2 above reagent circulation passage 9, the top surface opening of reaction cavity 4, reaction box body 2
The waste liquid tank 3 that top is surrounded with, the bottom of waste liquid tank 3 are provided with liquid outlet 6, are provided with reaction cavity 4 for fixing
The fixing groove 5 of section 7, is connected between reagent circulation passage 9 and reaction cavity 4 by reagent injection channel 8.
Inlet 1 and liquid outlet 6 are positioned at the opposite both sides of reaction box body 2.
Section 7 is multiple, and each section is being reacted along the direction of 1 side of inlet to 6 side of liquid outlet of reaction box body 2
It is sequentially distributed in cavity 4, the number of reagent injection channel 8 is corresponding with the number of section 7, the examination being emitted from reagent injection channel 8
Agent（Dewaxing agent）It is injected in 7 bottoms of corresponding section.
Reagent circulation passage 9 extends along the direction of 1 side of inlet to 6 side of liquid outlet of reaction box body 2, and reagent flow
The cross section of circulation passage 9 is sequentially reduced along the direction of 1 side of inlet to 6 side of liquid outlet of reaction box body 2.
The direction of 1 side of inlet to 6 side of liquid outlet of the cross section edge reaction box body 2 of reagent injection channel 8 is successively
Reduce.
The cross section of reagent injection channel 8 is arc.Reduce the residual of reaction reagent.
The top surface of reaction cavity 4 is higher than the top surface of waste liquid tank 3.
The open top face of reaction cavity 4 places mouth as sample.
As a preferred embodiment, 4 width of reaction cavity is 1mm~1000mm, and length is 1mm~1000mm, is highly
1mm~1000mm.The reagent injection channel 8 is made of 1~100 reagent being parallel to each other injection channel 8.The examination
Agent injection channel 8 and the angle of section 7 are 0 degree~89 degree, and length is 0.1mm~1000mm, and width is 0.1mm~1000mm.
The application method of this dewaxing reaction box is：
（1）Paraffin tissue sections are put into reaction cavity 4 and are fixed by fixing groove 5；
（2）Dewaxing agent is injected by reagent circulation passage 9, dewaxing agent is sprayed by reagent circulation passage 9 and each reagent
Passage 8 is sprayed to the bottom of paraffin tissue sections.Due to the inlet 1 one of the cross section edge reaction box body 2 of reagent circulation passage 9
The direction of side to 6 side of liquid outlet is sequentially reduced, and inlet 1 one of the cross section of reagent injection channel 8 along reaction box body 2
The direction of side to 6 side of liquid outlet is sequentially reduced so that the height for being ejected into paraffin tissue sections can be consistent, makes multiple sections
Reaction can wash away uniformly, simultaneous reactions can be carried out synchronously, and the top surface of dewaxing agent from the reaction cavity 1 of injection spills into useless
Liquid bath 3, since the top surface of reaction cavity 4 is higher than the top surface of waste liquid tank 3, enables the wax stone of surface flotation with dewaxing agent from reaction
The top of wall 2 flows into waste liquid tank 3.Then waste liquid is discharged from liquid outlet 6.
（3）The paraffin tissue sections after dewaxing are taken out after the completion of reaction；
As it can be seen that the present invention is suitable for the dewaxing treatment of various paraffin tissue sections, such as before immunofluorescence in situ hybridization
Processing.Structure of the present invention is foolproof.In the cost of reaction box, due to only needing a reaction box, and it is special without other
It is different to require, the cost of reaction box is greatly reduced, of the invention being truly realized is simple in structure, of low cost, and dewaxing is complete.
Specific embodiment described herein is only to spirit explanation for example of the invention.Technology belonging to the present invention is led
The technical staff in domain can do various modifications or additions to described specific embodiment or replace in a similar way
Generation, but without departing from spirit of the invention or beyond the scope of the appended claims.
Claims (1)
1. a kind of full-automatic tissue paraffin section de-waxing reaction box, including reaction box body (2), it is characterised in that the reaction
The bottom of box body (2) is provided with reagent circulation passage (9), and the side of reaction box body (2) bottom is provided with and reagent circulation passage
(9) inlet (1), the reaction cavity (4) being provided with reaction box body (2) above reagent circulation passage (9), reaction are connected
The top surface opening of cavity (4), reacts the waste liquid tank (3) being surrounded with the top of box body (2), and the bottom of waste liquid tank (3) is set
Have a liquid outlet (6), be provided with the fixing groove (5) for fixed section (7) in reaction cavity (4), reagent circulation passage (9) with it is anti-
Answer and connected by reagent injection channel (8) between cavity (4)；
Inlet (1) and liquid outlet (6) are positioned at the opposite both sides of reaction box body (2)；
(7) are cut into slices to be multiple, each section exists along the direction of inlet (1) side to liquid outlet (6) side of reaction box body (2)
It is sequentially distributed in reaction cavity (4), the number of reagent injection channel (8) is corresponding with the number of section (7), from reagent injection channel
(8) reagent of outgoing is injected in corresponding section (7) bottom；
Direction extension of the reagent circulation passage (9) along inlet (1) side to liquid outlet (6) side of reaction box body (2), and try
The cross section of agent circulation passage (9) subtracts successively along the direction of inlet (1) side to liquid outlet (6) side of reaction box body (2)
It is small；
Direction of the cross section of reagent injection channel (8) along inlet (1) side to liquid outlet (6) side of reaction box body (2)
It is sequentially reduced；
The cross section of reagent injection channel (8) is arc；
The top surface of reaction cavity (4) is higher than the top surface of waste liquid tank (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510920129.9A CN105352774B (en) | 2015-12-11 | 2015-12-11 | A kind of full-automatic tissue paraffin section de-waxing reaction box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510920129.9A CN105352774B (en) | 2015-12-11 | 2015-12-11 | A kind of full-automatic tissue paraffin section de-waxing reaction box |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105352774A CN105352774A (en) | 2016-02-24 |
| CN105352774B true CN105352774B (en) | 2018-04-20 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510920129.9A Active CN105352774B (en) | 2015-12-11 | 2015-12-11 | A kind of full-automatic tissue paraffin section de-waxing reaction box |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107389391B (en) * | 2017-05-11 | 2024-02-27 | 武汉康录生物技术股份有限公司 | Automatic dewaxing antigen repair system |
| CN112945657B (en) * | 2021-01-22 | 2023-08-25 | 中国科学院东北地理与农业生态研究所 | Pretreatment device and method for preparing plant material paraffin section |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2051854U (en) * | 1989-06-02 | 1990-01-31 | 赵国强 | Aftertreatment device for paraffin wax section |
| CN102517196A (en) * | 2011-12-31 | 2012-06-27 | 盛司潼 | Multi-channle sequencing reaction chamber and gene sequencing instrument |
| CN203101148U (en) * | 2013-01-30 | 2013-07-31 | 中南大学 | Staining box for immunohistochemistry |
| CN204330456U (en) * | 2015-01-13 | 2015-05-13 | 山东省立医院 | A kind of bone marrow smear chemical staining device box |
| CN104690044A (en) * | 2015-03-09 | 2015-06-10 | 北京普立泰科仪器有限公司 | Active type sampling probe washing device capable of preventing cross infection and cleaning method |
-
2015
- 2015-12-11 CN CN201510920129.9A patent/CN105352774B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2051854U (en) * | 1989-06-02 | 1990-01-31 | 赵国强 | Aftertreatment device for paraffin wax section |
| CN102517196A (en) * | 2011-12-31 | 2012-06-27 | 盛司潼 | Multi-channle sequencing reaction chamber and gene sequencing instrument |
| CN203101148U (en) * | 2013-01-30 | 2013-07-31 | 中南大学 | Staining box for immunohistochemistry |
| CN204330456U (en) * | 2015-01-13 | 2015-05-13 | 山东省立医院 | A kind of bone marrow smear chemical staining device box |
| CN104690044A (en) * | 2015-03-09 | 2015-06-10 | 北京普立泰科仪器有限公司 | Active type sampling probe washing device capable of preventing cross infection and cleaning method |
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| Publication number | Publication date |
|---|---|
| CN105352774A (en) | 2016-02-24 |
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| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Liu Kan Inventor after: Ai Zhao Inventor after: Ye Lun Inventor after: Xiang Jianzhen Inventor after: Chen Zhengqi Inventor after: Chen Junbo Inventor before: Liu Kan Inventor before: Ai Zhao Inventor before: Ye Lun Inventor before: Xiang Jianzhen Inventor before: Chen Zhengqi |
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| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20180102 Address after: 430075 building 4 building, Wuhan national biological industry base, No. 666, Wuhan national biological industry base, Wuhan, East Lake Development Zone, Hubei Province Applicant after: Wuhan Connecticut Biotechnology Limited by Share Ltd Address before: 430200 Jiangxia City, Wuhan province sunshine road, No. 1, Applicant before: Wuhan Textile University |
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