CN1053497A - Stablize the electrophoretic apparatus of electric field intensity on the holder - Google Patents
Stablize the electrophoretic apparatus of electric field intensity on the holder Download PDFInfo
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- CN1053497A CN1053497A CN 90105606 CN90105606A CN1053497A CN 1053497 A CN1053497 A CN 1053497A CN 90105606 CN90105606 CN 90105606 CN 90105606 A CN90105606 A CN 90105606A CN 1053497 A CN1053497 A CN 1053497A
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- holder
- voltage
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- electric field
- field intensity
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Abstract
The present invention has designed electric field intensity on a kind of stable holder, the electrophoretic apparatus of the accuracy of raising experimental result, repeatability, comparability, this device is characterized at two conductors of holder two ends increase, obtaining the voltage at holder two ends compares with normal voltage, if less than normal voltage, then increase the output voltage of electrophoresis power, if overgauge voltage, then reduce the output voltage of electrophoresis power, make holder both end voltage all-the-time stable on setting value.
Description
The invention belongs to biological chemistry (equipment) technical field, the electrophoretic apparatus of electric field intensity on specifically a kind of stable holder.
Electrophoresis just is found (1807, the Fecic experiment) as far back as the 19th-century initial stage, and nineteen thirty-seven Sweden scientist Tiselius makes electrophoresis apparatus, and is applied to separate and the research haemocyanin, thereby has begun electrophoretic techniques in biochemical Application for Field.Various types of in recent years electrophoretic techniques development are very rapid, and the electrophoresis tank that is suitable for all kinds electrophoresis also produces in succession.For the reliability and repeatability that increase the result, along with development of electronic technology, the electrophoresis of various high stabilities constantly emerges with voltage, current source.The intelligent electrophoresis instrument that has occurred computer control recently again.Although these electrophoresis have adopted very high consistent output voltage, output current, because the electrophoresis tank specification difference of each manufacturer production, and to same electrophoresis tank, also because each operation is different, or variation of temperature, the evaporation of moisture all may cause the variation of electrophoresis loop resistance, makes the allocation proportion of holder both end voltage in whole electrophoretic voltages change.Thereby cause the variation of electric field intensity on the holder, thereby influence electrophoretic velocity.But the variation of electrophoretic velocity 3% just can influence the repeatability of experimental result.
Purpose of the present invention then is the problem at above-mentioned existence, has designed the electric field intensity on a kind of stable holder, the electrophoretic apparatus of the accuracy of raising experimental result, repeatability, comparability.
The technology of the present invention solution: increase by two conductors at the holder two ends, obtaining the voltage at holder two ends compares with normal voltage, if less than normal voltage, then increase the output voltage of electrophoresis power, if overgauge voltage, then reduce the output voltage of electrophoresis power, make holder both end voltage all-the-time stable on setting value.
Advantage of the present invention: the electric field intensity on the stable holder that can be basic, degree of stability is not subjected to the influence of electrophoresis tank form, structure, specification and operating environment, only relevant with the voltage stabilizing electronic technology, can improve reliability, the repeatability of electrophoresis result, the comparability of electrophoresis result on the different size electrophoresis tank, present technique almost need not be changed and can implement existing electrophoresis apparatus production equipment.
Fig. 1 is existing electrophoresis apparatus schematic diagram.
Fig. 2 is an electrophoretic apparatus vertical view of the present invention.
Below in conjunction with accompanying drawing the present invention is further described:
The operation of electrophoresis apparatus at present is, on electrophoresis tank, connect electrode 4, inject damping fluid 3, put the holder 1 that is added with sample well, cost salt bridge 2, energized begins electrophoresis, when using electrophoresis apparatus of the present invention, only needs to cost the electrode 5 that newly adds of being drawn by the electrophoretic voltage power supply of the present invention at the holder two ends before energized and gets final product.The characteristics in electrophoretic voltage source are that sampling electrode is not connected on the output electrode two ends, and wherein an example is a photoelectric-isolated.
List of references:
(1) Chinese Medical Sciences University's Biochemistry Experiment in October, 1986
B William Si
(2) practical biochemical theory of the inferior volume of (English) K Weir and technology, Science Press 1974
(3) Zhongshan University's biochemical technology introduction, People's Health Publisher 1979
(4) check 1985(4 about the development Chinese medical of reactive loss phenomenon in the electrophoretic techniques and WD-II electrophoresis apparatus) 244
Claims (1)
1, the electrophoretic apparatus of electric field intensity on a kind of stable holder of being made up of holder, salt bridge, damping fluid, electrode, electrophoresis tank, electrophoresis power is characterized in that increasing at the holder two ends two conductors.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 90105606 CN1053497A (en) | 1990-01-15 | 1990-01-15 | Stablize the electrophoretic apparatus of electric field intensity on the holder |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 90105606 CN1053497A (en) | 1990-01-15 | 1990-01-15 | Stablize the electrophoretic apparatus of electric field intensity on the holder |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1053497A true CN1053497A (en) | 1991-07-31 |
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ID=4879416
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 90105606 Pending CN1053497A (en) | 1990-01-15 | 1990-01-15 | Stablize the electrophoretic apparatus of electric field intensity on the holder |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1053497A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110917877A (en) * | 2019-12-17 | 2020-03-27 | 吉林大学 | Orthogonal resonance electrophoresis device |
-
1990
- 1990-01-15 CN CN 90105606 patent/CN1053497A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110917877A (en) * | 2019-12-17 | 2020-03-27 | 吉林大学 | Orthogonal resonance electrophoresis device |
| CN110917877B (en) * | 2019-12-17 | 2023-10-24 | 吉林大学 | Orthogonal resonance electrophoresis device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |