CN105349711B - A kind of preparation method of transparent tree frog skin - Google Patents
A kind of preparation method of transparent tree frog skin Download PDFInfo
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- CN105349711B CN105349711B CN201510776823.8A CN201510776823A CN105349711B CN 105349711 B CN105349711 B CN 105349711B CN 201510776823 A CN201510776823 A CN 201510776823A CN 105349711 B CN105349711 B CN 105349711B
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- China
- Prior art keywords
- frog skin
- tree frog
- skin
- distilled water
- tree
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Classifications
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- C—CHEMISTRY; METALLURGY
- C14—SKINS; HIDES; PELTS; LEATHER
- C14C—CHEMICAL TREATMENT OF HIDES, SKINS OR LEATHER, e.g. TANNING, IMPREGNATING, FINISHING; APPARATUS THEREFOR; COMPOSITIONS FOR TANNING
- C14C3/00—Tanning; Compositions for tanning
Abstract
The invention provides a kind of preparation method of transparent tree frog skin.The present invention is with tree frog skin as raw material, using trypsin hydrolysis method, the blackish green on its surface is effectively removed, obtain transparent quality and retain the tree frog skin of original special texture, the New-type leather raw material for being easy to dyeing is become, while for technological design provides new material.Preparation method of the present invention realizes the effective utilization to tree frog skin, and preparation process has broad application prospects beneficial to environmental protection.
Description
Technical field
The invention belongs to tanning technology field, and in particular to the tanning technology of tree frog skin, more particularly to a kind of transparent wood frog
The enzyme bating hydrolysis preparation method of skin.
Technical background
Leather tanning is a kind of leather processing technique of relative maturity, is primarily referred to as carrying out tan to ox-hide, sheepskin, fish-skin etc.
Make the technology of processing.In Application No. WO2015/127935 A1《Method for tanning hides and tanned
leather produced by means of said method》Patent document in, disclose a kind of leather tanning method,
Control to pH range in tannery is studied and inquired into.Zhang Mei, Dong Henan etc. are in Application No.
201210364423.2《The process hides processing technology of Dongbei forest-frog skin》Patent document in, disclose a kind of tanning side of tree frog skin
Method, will the tanning of tree frog skin raw hide into bark, make it have certain practicality, realize to a certain extent to tree frog skin
Effectively utilize.The purpose of the invention is that tree frog skin is applied to the preparation of clothes raw material, but because one side tree frog skin is black green in itself
The affect trait of color its Color, on the other hand mechanical performance of the heavy tannery necessarily to tree frog skin finished product cause
Adverse effect, process for tanning is unfavorable for environmental protection in addition, and the invention preparation method is obviously further improved and perfect.
The proterties of tree frog skin blackish green masks tree frog skin texture in itself to a certain extent, but has no public up to now
The correlation technique of kairine frog skin Vitrification management.Therefore, how tree frog skin is removed in itself on the basis of the existing tannin technique
Blackish green, makes it show native texture, and then be the technology for requiring study at present as the New-type leather raw material of dyeing is easy to
Problem.
The content of the invention
The present invention is intended to provide a kind of preparation method of transparent tree frog skin.Specifically, the present invention uses trypsase water
The protein on solution tree frog skin surface, removes the blackish green on its surface, it is showed the transparent proterties of native texture, and then turn into
It is easy to the New-type leather raw material of dyeing；Meanwhile, preparation method of the present invention realizes the effective utilization to tree frog skin, is technique
Design provides a kind of new material, and preparation process is conducive to environmental protection.
The present invention is adopted the following technical scheme that to solve technical problem：
A kind of preparation method of transparent tree frog skin, comprises the following steps：
(1) take tree frog skin to be immersed in distilled water, temperature is 10~24 DEG C, stands 3.0~24.0h, adds sodium chloride, is made
Sodium chloride is 1: 5~40 with distilled water mass ratio, is stood, and is warming up to 22~34 DEG C, is incubated 0.2~2.0h；
(2) tree frog skin after step (1) treatment is taken out, with distilled water flushing, removes the limbs skin and table of tree frog skin
After the debris such as layer residual fat, remaining tree frog skin is weighed, and be immersed in the hydrogen-oxygen that mass percentage concentration is 0.3%~1.5%
Change in sodium water solution, 2.0~6.0h is stood after stirring, add 0.1mol/L ammonium sulfate solutions, regulation solution pH value is 6.0
~9.5,0.5~2.0h is stood after stirring；
(3) tree frog skin after step (2) treatment is taken out, with distilled water flushing after, be put into mass percentage concentration for 0.2%
In~1.0% trypsase aqueous solution, hydrolysis temperature is 24~37 DEG C, 0.5~2.0h of hydrolysis time, takes out tree frog skin and steams
Distilled water is rinsed, and is put after shady place dries, and obtains final product transparent tree frog skin.
Preferably, the temperature that distilled water is immersed in described in step (1) is 12~18 DEG C, 6.0~18.0h is stood, plus
The sodium chloride for entering is 1: 15~30 with distilled water mass ratio, is warming up to 28~32 DEG C, is incubated 0.2~1.5h.
Preferably, the concentration of sodium hydrate aqueous solution described in step (2) is 0.6%~1.2%, 0.1mol/L is added
Ammonium sulfate solution, regulation solution pH value is 7.8~8.5.
Preferably, the mass percentage concentration of the trypsase aqueous solution described in step (3) is 0.4%~0.8%.
The present invention has the advantages that compared with prior art：
The present invention, using trypsin hydrolysis method, can effectively remove the black of its surface with northeast tree frog skin as raw material
Green, obtains transparent quality and retains the tree frog skin of original special texture, for technological design provides a kind of new material, while carrying
A kind of New-type leather raw material be easy to and dye is supplied；Preparation method of the present invention realizes the effective utilization to tree frog skin, and
Preparation process is conducive to environmental protection, has broad application prospects.
Brief description of the drawings
Fig. 1 is the photo of tree frog skin raw material
Fig. 2 is the photo that example 1 prepares tree frog skin
Fig. 3 is the photo that example 2 prepares tree frog skin
Fig. 4 is the photo that example 3 prepares tree frog skin
Fig. 5 is the photo with transparent plastic film as reference substance
Specific embodiment
Below by embodiment, the present invention is further described, but these embodiments are only used for explaining the present invention, to this hair
Bright scope is not construed as limiting.
Embodiment 1
(1) tree frog skin (as shown in Figure 1) 200g is taken, is immersed in the beaker equipped with 1000ml distilled water, beaker is put into
In insulating box, regulation temperature is 10 DEG C, stands insulation 3.0h, adds sodium chloride 25.362g, makes sodium chloride and distilled water mass ratio
It is 1: 40, stands, be warming up to 22 DEG C, is incubated 0.2h；
(2) step (1) gained tree frog skin is taken out, with distilled water flushing, limbs skin and the top layer for removing tree frog skin are residual
After staying the debris such as fat, it is 48.563g to weigh remaining tree frog skin quality, is immersed in the hydroxide that mass percentage concentration is 0.3%
In sodium water solution, 2.0h is stood after stirring, add 0.1mol/L ammonium sulfate solutions, regulation solution pH value is 6.0, after stirring
Stand 0.5h.
(3) by step (2) gained tree frog skin take out, with distilled water flushing after, be put into the pancreas that mass percentage concentration is 0.2%
In aqueous solution of protease, regulation temperature is 24 DEG C, hydrolyzes 0.5h, takes out tree frog skin distilled water flushing, is put after shady place dries,
Transparent tree frog skin is obtained final product, as shown in Figure 2；Take simultaneously and do not place the base plate of tree frog skin as control, as shown in Figure 5.
Embodiment 2
(1) tree frog skin 200g is taken, is immersed in the beaker equipped with 1000ml distilled water, beaker is put into insulating box, temperature
Degree is adjusted to 15 DEG C, stands insulation 12.0h, adds sodium chloride 40.382g, makes sodium chloride be 1: 25 with distilled water mass ratio, quiet
Put, be warming up to 30 DEG C, be incubated 0.5h；
(2) step (1) gained tree frog skin is taken out, with distilled water flushing, limbs skin and the top layer for removing tree frog skin are residual
After staying the debris such as fat, it is 52.739g to weigh remaining tree frog skin quality, is immersed in the hydroxide that mass percentage concentration is 0.9%
In sodium water solution, 4.0h is stood after stirring, add 0.1mol/L ammonium sulfate solutions, regulation solution pH value is 8.5, after stirring
Stand 1.0h；
(3) by step (2) gained tree frog skin take out, with distilled water flushing after, be put into the pancreas that mass percentage concentration is 0.6%
In aqueous solution of protease, regulation temperature is 37 DEG C, hydrolyzes 1.0h, takes out tree frog skin distilled water flushing, is put after shady place dries,
Transparent tree frog skin is obtained final product, as shown in Figure 3.
Embodiment 3
(1) tree frog skin 200g is taken, is immersed in the beaker equipped with 1000ml distilled water, beaker is put into insulating box, temperature
Degree is adjusted to 24 DEG C, stands insulation 24.0h, adds sodium chloride 200.102g, makes sodium chloride be 1: 5 with distilled water mass ratio, quiet
Put, add warm water to be warming up to 34 DEG C, be incubated 2.0h；
(2) step (1) gained tree frog skin is taken out, with distilled water flushing, limbs skin and the top layer for removing tree frog skin are residual
After staying the debris such as fat, it is 46.873g to weigh remaining tree frog skin quality, is immersed in the hydroxide that mass percentage concentration is 1.5%
In sodium water solution, 6.0h is stood after stirring, add 0.1mol/L ammonium sulfate solutions, regulation solution pH value is 9.5, after stirring
Stand 2.0h.
(3) by step (2) gained tree frog skin take out, with distilled water flushing after, be put into the pancreas that mass percentage concentration is 1.0%
In aqueous solution of protease, regulation temperature is 30 DEG C, hydrolyzes 2.0h, takes out tree frog skin distilled water flushing, is put after shady place dries,
Transparent tree frog skin is obtained final product, as shown in Figure 4.
Embodiment 4
(1) tree frog skin 200g is taken, is immersed in the beaker equipped with 1000ml distilled water, beaker is put into insulating box, temperature
Degree is adjusted to 12 DEG C, stands insulation 6.0h, adds sodium chloride 33.421g, makes sodium chloride be 1: 30 with distilled water mass ratio, quiet
Put, add warm water to be warming up to 28 DEG C, be incubated 0.2h；
(2) step (1) gained tree frog skin is taken out, with distilled water flushing, limbs skin and the top layer for removing tree frog skin are residual
After staying the debris such as fat, it is 51.302g to weigh remaining tree frog skin quality, is immersed in the hydroxide that mass percentage concentration is 0.6%
In sodium water solution, 3.0h is stood after stirring, add 0.1mol/L ammonium sulfate solutions, regulation solution pH value is 7.8, after stirring
Stand 0.7h；
(3) by step (2) gained tree frog skin take out, with distilled water flushing after, be put into the pancreas that mass percentage concentration is 0.4%
In aqueous solution of protease, regulation temperature is 28 DEG C, hydrolyzes 0.5h, takes out tree frog skin distilled water flushing, is put after shady place dries,
Obtain final product transparent tree frog skin.
Embodiment 5
(1) tree frog skin 200g is taken, is immersed in the beaker equipped with 1000ml distilled water, beaker is put into insulating box, temperature
Degree is adjusted to 18 DEG C, stands insulation 18.0h, adds sodium chloride 66.712g, makes sodium chloride be 1: 15 with distilled water mass ratio, quiet
Put, add warm water to be warming up to 32 DEG C, be incubated 1.5h；
(2) step (1) gained tree frog skin is taken out, with distilled water flushing, limbs skin and the top layer for removing tree frog skin are residual
After staying the debris such as fat, it is 51.361g to weigh remaining tree frog skin quality, is immersed in the hydroxide that mass percentage concentration is 1.2%
In sodium water solution, 5.0h is stood after stirring, add 0.1mol/L ammonium sulfate solutions, regulation solution pH value is 8.5, after stirring
Stand 1.5h；
(3) by step (2) gained tree frog skin take out, with distilled water flushing after, be put into the pancreas that mass percentage concentration is 0.8%
In aqueous solution of protease, regulation temperature is 34 DEG C, hydrolyzes 1.5h, takes out tree frog skin distilled water flushing, is put after shady place dries,
Obtain final product transparent tree frog skin.
Related description on accompanying drawing cited in the embodiment of the present invention：
Fig. 1 is positioned over the base plate of black and white strip by the tree frog skin raw material without Vitrification management that embodiment 1-4 is used
On photo, the subcutaneous base plate striped of wood frog is completely invisible, it was demonstrated that tree frog skin raw material is completely opaque.
Fig. 2 is the photo that transparent tree frog skin prepared by embodiment 1 is positioned on the base plate of black and white strip, base plate stripe portion
It can be seen that, illustrate that its transparent effect is poor.
Fig. 3 is the photo that transparent tree frog skin prepared by embodiment 2 is positioned on the base plate of black and white strip, base plate clear-cut texture
It can be seen that, it was demonstrated that its transparent effect is preferable, and intact unbroken.
Fig. 4 is the photo that the transparent tree frog skin prepared according to embodiment 3 is positioned on the base plate of black and white strip, base plate striped
High-visible, transparent effect is preferable, but substantially breakage occurs in tree frog skin, sufficiently complete, shows that its mechanical performance is poor.
Fig. 5 be black and white strip base plate on place layer of transparent plastic sheeting as reference substance photo, in this, as upper
State the tester of tree frog skin transparent effect experiment.
Claims (2)
1. a kind of preparation method of transparent tree frog skin, it is characterised in that comprise the following steps：
(1) take tree frog skin to be immersed in distilled water, temperature is 12~18 DEG C, stand 6.0~18.0h, sodium chloride and the steaming of addition
The mass ratio of distilled water is 1：15~30,28~32 DEG C are warming up to, it is incubated 0.2~1.5h；
(2) tree frog skin after step (1) treatment is taken out, with distilled water flushing, limbs skin and the top layer for removing tree frog skin are residual
After staying the debris such as fat, remaining tree frog skin is weighed, and be immersed in the NaOH that mass percentage concentration is 0.6%~1.2%
In the aqueous solution, 2.0~6.0h being stood after stirring, add 0.1mol/L ammonium sulfate solutions, regulation solution pH value is 7.8~
8.5,0.5~2.0h is stood after stirring；
(3) tree frog skin after step (2) treatment is taken out, with distilled water flushing after, be put into mass percentage concentration for 0.2%~
In the 1.0% trypsase aqueous solution, hydrolysis temperature is 24~37 DEG C, 0.5~2.0h of hydrolysis time, takes out tree frog skin distillation
Water is rinsed, and is put after shady place dries, and obtains final product transparent tree frog skin.
2. a kind of preparation method of transparent tree frog skin according to claim 1, it is characterised in that pancreas egg described in step (3)
The mass percentage concentration of white enzyme aqueous solution is 0.4%~0.8%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510776823.8A CN105349711B (en) | 2015-11-13 | 2015-11-13 | A kind of preparation method of transparent tree frog skin |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510776823.8A CN105349711B (en) | 2015-11-13 | 2015-11-13 | A kind of preparation method of transparent tree frog skin |
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| Publication Number | Publication Date |
|---|---|
| CN105349711A CN105349711A (en) | 2016-02-24 |
| CN105349711B true CN105349711B (en) | 2017-07-04 |
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| CN201510776823.8A Expired - Fee Related CN105349711B (en) | 2015-11-13 | 2015-11-13 | A kind of preparation method of transparent tree frog skin |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112322807A (en) * | 2020-10-22 | 2021-02-05 | 四川大学 | Method for preparing transparent leather based on solution refractive index |
| CN112322806B (en) * | 2020-10-22 | 2021-10-08 | 四川大学 | Method for producing transparent leather based on organic tanning agent |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| BR0103938B1 (en) * | 2001-09-06 | 2011-05-03 | welded frog leather blankets, welded frog leather blanket manufacturing process, welded fish leather blankets and welded fish leather blanket manufacturing process. | |
| CN1208470C (en) * | 2001-12-20 | 2005-06-29 | 单凯波 | Processing method of making leather using frog skin |
| RU2553244C2 (en) * | 2013-10-25 | 2015-06-10 | Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Астраханский государственный технический университет" (ФГБОУ ВПО АГТУ) | Method of processing skin of lake frog |
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