CN105349477A - Leuconostoc mesenteroides as well as preparation method and application thereof - Google Patents
Leuconostoc mesenteroides as well as preparation method and application thereof Download PDFInfo
- Publication number
- CN105349477A CN105349477A CN201510967496.4A CN201510967496A CN105349477A CN 105349477 A CN105349477 A CN 105349477A CN 201510967496 A CN201510967496 A CN 201510967496A CN 105349477 A CN105349477 A CN 105349477A
- Authority
- CN
- China
- Prior art keywords
- leuconostoc mesenteroides
- culture
- tyc
- substratum
- cgmccno
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/1203—Addition of, or treatment with, enzymes or microorganisms other than lactobacteriaceae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses Leuconostoc mesenteroides as well as a preparation method and an application of the Leuconostoc mesenteroides in polysaccharide production. The Leuconostoc mesenteroides is collected in CGMCC (China General Microbiological Culture Collection Center), the collection number is CGMCC No. 10064, and the culture name is BD3749. The Leuconostoc mesenteroides can be used for large-scale preparation of insoluble polysaccharides, so that whey separation can be controlled better, the Leuconostoc mesenteroides can be applied to fermentation of dairy products, and influence on the quality and shelf life of the dairy products due to severe damage to texture of the products because of whey separation during storage of the dairy products can be avoided. A strain culture method is simple and easy to operate, large quantity of insoluble polysaccharides is prepared from the Leuconostoc mesenteroides, and the Leuconostoc mesenteroides is suitable for industrialized application and has broad market.
Description
Technical field
The present invention relates to microorganism field, be specifically related to a kind of Leuconostoc mesenteroides and its preparation method and application.
Background technology
Leuconostoc mesenteroides is the important bacterial classification of the leuconos toc in milk-acid bacteria.U.S. FDA and AAFCO were classified as one of 42 kinds of safe microorganisms directly can eating (raising) in 1989.Leuconostoc mesenteroides subsp mesenteroides has also been listed in " can be used for the bacterial classification list of food " by the Ministry of Health of China in 2012.
Research confirms, Leuconostoc mesenteroides not only has the characteristic of the high acid ability of ordinary lactic acid bacteria, resistance of oxidation and the pathogenic bacterium that fly up and down, and also has the ability that can produce abundant species, a fairly large number of exocellular polysaccharide in fermentation.Leuconostoc mesenteroides is also found extensively to be distributed in leavened food, particularly fermented dairy prod, pickle in pickles and making fruit wine.
The dextran that Leuconostoc mesenteroides fermentation Sucrose synthesis is a large amount of is the raw material of the main component-dextran producing plasma substitute.Medically very important, having the effect maintaining colloidal osmotic pressure and increase blood molten amount, is one of excellent plasma substitute of generally acknowledging at present, is usually used in clinically treating owing to losing blood, wound, burn and the hemorrhagic shock caused such as poisoning.Research along with Leuconostoc mesenteroides exocellular polysaccharide is more and more comprehensive and go deep into, and the functional oligose product synthesized by Leuconostoc mesenteroides is expected to realize industrialization and produces.
At present, domestic existing Leuconostoc mesenteroides mainly produces soluble polysaccharide, does not produce insolubility polysaccharide or insolubility polysaccharide yield is very low, so lack the new strains that can produce insolubility polysaccharide in a large number.
Summary of the invention
Technical problem to be solved by this invention is, mainly soluble polysaccharide is produced for existing Leuconostoc mesenteroides, do not produce insolubility polysaccharide or the very low present situation of insolubility polysaccharide yield, the invention provides a strain Leuconostoc mesenteroides (Leuconostocmesenteroides) and cultural method thereof and application.Described Leuconostoc mesenteroides can prepare polysaccharide, prepares insoluble polysaccharide especially in a large number, can be applied to better in Dairy fermentation and storage.
One of technical scheme of the present invention is: a kind of Leuconostoc mesenteroides (Leuconostocmesenteroides), and it is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and preserving number is: CGMCCNo.10064.
Pickle pickles from the farmers' of Heilongjiang Province and be isolated, the Leuconostoc mesenteroides CGMCCNo.10064 described in acquisition.Identify this bacterial strain, result is Leuconostoc mesenteroides Leuconostocmesenteroides, called after BD3749.This bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on November 26th, 2014, and receives preservation center and to register on the books numbering CGMCCNo.10064.
Two of technical scheme of the present invention is: a kind of method preparing described Leuconostoc mesenteroides CGMCCNo.10064, and it comprises the following steps: to cultivate described Leuconostoc mesenteroides CGMCCNo.10064 in the medium.
Wherein, described substratum is the substratum of this area routine, can grow described Leuconostoc mesenteroides CGMCCNo.10064.Be preferably TYC substratum, described TYC substratum is the TYC substratum of this area routine, preferably comprises following component: tryptone (or casein hydrolyzate) 1.5g; Yeast extract paste 0.5g; CYSTINE 0.02g; Sodium acetate 2.0g; Na
2sO
40.01g; NaCl0.1g; Sucrose 5.0g; Distilled water 100ml; If be TYC solid medium, then except said components, preferably also comprise the agar of 1.5-2.0 gram (g), more preferably comprise 1.8g agar.
The temperature of described cultivation is the temperature of this area routine, can grow described Leuconostoc mesenteroides CGMCCNo.10064, is preferably 25 ~ 37 DEG C, is more preferably 30 ~ 37 DEG C, and the best is 30 DEG C.The time of described cultivation is the time of this area routine, is preferably 18 ~ 72 hours (h), is more preferably 18 hours (h) or 72 hours (h), is best 18 hours (h).The pH of described cultivation is the pH of this area routine, can grow described Leuconostoc mesenteroides CGMCCNo.10064, is preferably 4 ~ 6, is more preferably 4 ~ 5.
Culture condition of the present invention is this area conventional culture conditions, is preferably aerobic quiescent culture, and concussion is cultivated or anaerobism quiescent culture, and the concussion speed that described concussion is cultivated is conventional, is preferably 180r/min.
Preferably, the step using seed culture medium to carry out seed culture is also comprised before described cultivation.Described seed culture is the seed culture of this area routine.Described seed culture medium is the seed culture medium of this area routine, is preferably TYC substratum; The time of described seed culture is the time of this area routine, is preferably 16 ~ 32h, is more preferably 16 ~ 24h, and the best is 24h; The temperature of described seed culture is the temperature of this area routine, is preferably 28 ~ 32 DEG C, is more preferred from 30 DEG C.The inoculum size of described seed culture is the inoculum size of this area routine, and be preferably 1 ~ 2%, be more preferred from 1%, described per-cent is volume percent.
Three of technical scheme provided by the invention is: the application of described Leuconostoc mesenteroides CGMCCNo.10064 in exocellular polysaccharide is produced.Described exocellular polysaccharide comprise solubility with insoluble.
On the basis meeting this area general knowledge, above-mentioned each optimum condition, can arbitrary combination, obtains the preferred embodiments of the invention.
Agents useful for same of the present invention and raw material are all commercially.
Positive progressive effect of the present invention is: Leuconostoc mesenteroides provided by the invention (Leuconostocmesenteroides) can prepare polysaccharide, especially can prepare insolubility polysaccharide in a large number, thus can control whey precipitation better.Therefore the insolubility polysaccharide that described bacterial strain and this bacterial strain are prepared and obtained all can be used in Dairy fermentation, especially in solid type protein product (as cheese) preparation, play the effect of peptizer, also can avoid because whey separates out the matter structure generation havoc causing product in dairy product storage, thus affect its quality and shelf-lives.Described strain culturing method is simple to operation, and its insolubility polysaccharide amount prepared and obtain is large, is applicable to commercial application, has wide market.
biomaterial preservation information
Leuconostoc mesenteroides BD3749 of the present invention, be deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC) on November 26th, 2014, preservation address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica, postcode: 100101, deposit number is: CGMCCNo.10064, culture title is BD3749, and Classification And Nomenclature is Leuconostoc mesenteroides Leuconostocmesenteroides.
Accompanying drawing explanation
Fig. 1 is the colonial morphology that Leuconostoc mesenteroides BD3749 of the present invention cultivates on TYC.
Fig. 2 is the contrast of Leuconostoc mesenteroides BD3749 of the present invention and reference culture Leuconostoc mesenteroides ATCC 10830 a colonial morphology.
Embodiment
Mode below by embodiment further illustrates the present invention, but does not therefore limit the present invention among described scope of embodiments.The experimental technique of unreceipted actual conditions in the following example, conventionally and condition, or selects according to catalogue.
Medium component used by the present invention is as follows:
TYC substratum (liquid TYC substratum): Tryptones 1.5g; Yeast extract paste 0.5g; CYSTINE 0.02g; Sodium acetate 2.0g; Na
2sO
40.01g; NaCl0.1g; Sucrose 5.0g; Distilled water 100ml; 115 DEG C, sterilizing 15min.
TYC solid medium: liquid TYC substratum separately adds 1.8g agar.
The separation of embodiment 1 bacterial strain BD3749
Pickles are pickled by the farmers' of getting Heilongjiang Province, get 1g with sterile manner, immerse in stroke-physiological saline solution, get leach liquor and carry out gradient dilution, are spread evenly across on solid TYC substratum by the mode of coating by diluent, cultivate 24-48h for 30 DEG C.Choose sticky nasal mucus shape, to have single bacterium colony of good stringiness multiple, be transferred to respectively on new solid TYC substratum, obtain the bacterium colony of multiple purifying, by one of them single bacterium colony called after BD3749.
The feature of embodiment 2 bacterial strain BD3749
1, colony characteristics
Get single bacterium colony of bacterial strain BD3749, be transferred on TYC solid medium (agar), cultivate 24h in 37 DEG C of anaerobic box constant incubators after, observe the features such as the size of its bacterium colony, color, edge, projection, slickness, viscosity, transparency.Result as shown in Figure 1.Result shows, bacterial strain BD3749 forms sticky nasal mucus shape, have good stringiness, neat in edge, projection, smooth, colourless opaque single bacterium colony.
2, morphological observation and physiological and biochemical property
Picking, at the upper fresh culture thing cultivating 24h of TYC solid medium (agar), carries out Physiology and biochemistry test.Result shows, and BD3749 is gram-positive cocci, amphimicrobian.Catalase experiment is negative, oxidase negative.
3, API50CH identification mark
API50CH identification systems (production firm: bioMe ' rieux) are utilized to measure the product acid situation of bacterial strain BD3749 fermentable carbohydrates.In the reagent strip of API50CH, the carbon source that different sequence numbers is corresponding different, meanwhile, wherein containing indicator, like this, if the carbon source of its correspondence is utilized, then nutrient solution pH declines, and indicator just can change color, is beneficial to observed and recorded.
API50CH identification systems operation steps is as follows:
1) API50CH basic media components: Tryptones 1g, yeast extract paste 0.5g, ammonium sulfate 2g, phenol red 0.18g, inorganic salt basis (Cohen-Bazire) 10ml, phosphate buffered saline buffer (pH7.8) 1000ml.
2) on flat board, cultivate bacterial strain BD3749, picking has multiple size close, the flat board that single bacterium colony is separated.The close single bacterium colony of picking size several, add 1) in substratum in, make OD
600the bacteria suspension of=0.4 ~ 0.6.
3) joining taking over the API50CH substratum of planting containing testing in the tubule of bar, covering the sterilized paraffin oil of one deck above.
4) aerobic cultivation in the constant incubator of 30 DEG C, records experimental phenomena at 24h and 48h respectively.
Bacterial strain BD3749 utilizes the result of carbohydrate fermentation product acid as shown in table 1.
The API50CH qualification result of table 1. bacterial strain BD3749
"+" expression utilizes sugar to produce acid, and "-" expression does not produce acid.
4,16srDNA sequence homology analysis
The BD3749 bacterial strain 2-3 articulating of picking TYC slat chain conveyor enters in 30ml liquid TYC substratum, at being placed in 30 DEG C, and 180r/min shaking culture 18h.The centrifugal 5min of 12000rpm collects thalline.Test kit (sky root is biological) is utilized to extract the STb gene of bacterial strain as gene amplification template, with bacterium 16srDNA universal primer, 27f:5 '-agagtttgatcctggctcag-3 ' (SEQIDNO:1), 1492r:5 '-taccttgttacgactt-3 ' (SEQIDNO:2) carry out PCR reaction.Response procedures is: 94 DEG C of denaturation 5min; 94 DEG C of sex change 30s, 55 DEG C of annealing 30s, 72 DEG C extend 2min, 30 circulations; 72 DEG C extend 8min.Amplified production 1% (w/v) agarose gel electrophoresis detects, and reclaims target stripe and serves the raw work order-checking of marine life.Gained nucleotide sequence (as shown in SEQIDNO:3) is by the online Blast comparison in American National Biotechnology Information center ncbi database (http://www.ncbi.nlm.nih.gov).Result show, with 16srDNA sequence (SEQIDNO:3) similarity of bacterial strain BD3749 the highest be Leuconostocmesenteroidessubsp.mesenteroidesJ18, similarity is 99%.
5, pheS gene homology is analyzed
The BD3749 STb gene extracted in 4 is as gene amplification template, with bacterium pheS primer, pheS-f:5 '-atgggcttaattgaagatct-3 ' (SEQIDNO:4), pheS-r:5 '-tcagttcccctttcgattgaat-3 ' (SEQIDNO:5) carry out PCR reaction.Response procedures is: 94 DEG C of denaturation 5min; 94 DEG C of sex change 30s, 55 DEG C of annealing 30s, 72 DEG C extend 2min, 30 circulations; 72 DEG C extend 8min.Amplified production 1% (w/v) agarose gel electrophoresis detects, and reclaims target stripe and serves the raw work order-checking of marine life.Gained nucleotide sequence (as shown in SEQIDNO:6) is by the online Blast comparison in American National Biotechnology Information center ncbi database (http://www.ncbi.nlm.nih.gov).Result shows, and that pheS gene order (SEQIDNO:6) similarity of bacterial strain BD3749 is the highest is Leuconostocmesenteroidessubsp.mesenteroidesJ18, and similarity is 99%.
Result in integrated embodiment 2, thinks that bacterial strain BD3749 belongs to the new strains of Leuconostocmesenteroides.
Leuconostoc mesenteroides BD3749 of the present invention, be deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC) on November 26th, 2014, deposit number is: CGMCCNo.10064, and Classification And Nomenclature is Leuconostoc mesenteroides Leuconostocmesenteroides.
The application of embodiment 3 Leuconostoc mesenteroides (Leuconostocmesenteroides) BD3749
(1) preparation of Leuconostoc mesenteroides (Leuconostocmesenteroides) BD3749 fermentation and fermented liquid
Prepare the lyophilized powder of Leuconostoc mesenteroides BD3749: picking 2 bacterium colonies, cultivate 4h for 30 DEG C in the skimming milk solution being inoculated in 10% (w/w) of 300ml ,-80 DEG C of pre-freezes are spent the night ,-20 DEG C of lyophilizes.
The lyophilized powder 100mg of Leuconostoc mesenteroides BD3749 is dissolved with 0.1ml sterile distilled water, lines on TYC solid medium with transfering loop picking one ring, take out after 30 DEG C of aerobic quiescent culture 24h.
Single bacterium colony in picking TYC solid medium, be transferred in 20ml liquid TYC substratum, 30 DEG C of aerobic quiescent culture 24h, obtain seed liquor.Be transferred in new liquid TYC nutrient solution by seed liquor in the ratio of inoculum size 1% (v/v), 30 DEG C of aerobic quiescent culture 72h, obtain fermented liquid.
(2) preparation of exocellular polysaccharide crude product in fermented liquid
By the fermented liquid of step (1) gained at 100 DEG C of heating 10min, be cooled to room temperature, this solution is labeled as solution 1.The centrifugal 20min of 9000rpm, get 95% ethanolic soln that supernatant liquor adds 3 times of volumes, this solution is labeled as solution 2.Solution 2 puts 4 DEG C of hold over night, 9000rpm centrifugal 20min collecting precipitation thing, lyophilize, and detect through Phenol-sulphate acid method, in gained throw out, more than 90% is polysaccharide, obtains solubility Crude polysaccharides.
The centrifugal precipitation obtained of solution 1, is resuspended in the NaOH aqueous solution containing 1M.Then, the centrifugal 20min of 9000rpm, gets supernatant liquor.In got supernatant liquor, add 95% ethanolic soln of 3 times of volumes.4 DEG C of hold over night, 9000rpm centrifugal 20min collecting precipitation thing, lyophilize, detect through Phenol-sulphate acid method, in gained throw out, more than 90% is polysaccharide, obtains insolubility Crude polysaccharides.
The bacterial strain BD3749 fermentation insolubility polysaccharide of gained and the output ratio (w/w) of soluble polysaccharide: 4:5.
The application of embodiment 4 Leuconostoc mesenteroides (Leuconostocmesenteroides) BD3749
The lyophilized powder 100mg of Leuconostoc mesenteroides ATCC 10830 a and Leuconostoc mesenteroides BD3749 is dissolved respectively with 0.1ml sterile distilled water, lines on TYC solid medium respectively with transfering loop picking one ring, take out after 30 DEG C of aerobic quiescent culture 24h.Picking list bacterium colony, be inoculated into sterilizing (115 DEG C, 15min) containing 5% (w/v) sucrose 100ml pure milk in, 30 DEG C of aerobic standing for fermentation are after 2 days, be placed in 4 DEG C of refrigerators, pick and place respectively and put 7 days, the sample after 14 days, by liquid pouring out measurement volumes, observe liquid volume change.Result is as shown in table 2.
Table 2. bacterial strain BD3749 and ATCC10830a pure milk fermentating liquid volume time synopsis
From upper table result, bacterial strain BD3749 adds placement after fermentation in milk, gained liquid volume reduces along with time lapse, proves that bacterial strain BD3749 controls the precipitation phenomenon of whey well, by contrast the precipitation of strains A TCC10830 then uncontrollable whey.
In actual applications, because the major protein inside milk is inside whey, generally make solid type protein product (as cheese) by adding peptizer control whey precipitation.Also all can there is the problem that whey is separated out in dairy product, whey precipitation is more will be caused the matter structure generation havoc of product thus affect its quality and shelf-lives in storage and shelf-lives.Bacterial strain BD3749 of the present invention self just can control the precipitation of whey, can be applied to the preparation of milk-product, have commercial application value.
The preparation of the exocellular polysaccharide of comparative example 1 Leuconostoc mesenteroides (Leuconostocmesenteroides) ATCC10830a
Lyophilized powder (preparation method is with the preparation method of the lyophilized powder of the Leuconostoc mesenteroides BD3749) 100mg of Leuconostoc mesenteroides ATCC 10830 a is dissolved with 0.1ml sterile distilled water, line on TYC solid medium with transfering loop picking one ring, take out after 30 DEG C of aerobic quiescent culture 24h.
With reference to the method for embodiment 3, preparation solubility and insolubility polysaccharide.Result shows, and Leuconostoc mesenteroides ATCC 10830 a is after preparing the step of insolubility Crude polysaccharides in embodiment 3, cannot be precipitated thing, namely Leuconostoc mesenteroides ATCC 10830 a does not produce insolubility polysaccharide.
Comparative example 2 Leuconostoc mesenteroides (Leuconostocmesenteroides) BD3749 and Leuconostoc mesenteroides ATCC 10830 a morphological specificity contrast
The Leuconostoc mesenteroides ATCC 10830 a inoculated by embodiment 4 method and Leuconostoc mesenteroides BD3749 point are seeded on same TYC flat board.Result as shown in Figure 2.Can find out, Leuconostoc mesenteroides BD3749 is owing to producing insoluble polysaccharide, and bacterium colony is opaque.And Leuconostoc mesenteroides ATCC 10830 a does not produce insoluble polysaccharide, bacterium colony is transparent.
Should be understood that those skilled in the art can make various changes or modifications the present invention after having read foregoing of the present invention, these equivalent form of values fall within the application's appended claims limited range equally.
Claims (10)
1. a Leuconostoc mesenteroides (Leuconostocmesenteroides), is characterized in that, it is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and preserving number is: CGMCCNo.10064.
2. prepare a method of Leuconostoc mesenteroides CGMCCNo.10064, it is characterized in that, it comprises the following steps: to cultivate Leuconostoc mesenteroides CGMCCNo.10064 in the medium.
3. method as claimed in claim 2, it is characterized in that, described substratum is TYC substratum.
4. method as claimed in claim 3, it is characterized in that, described TYC substratum also comprises the agar of 1.5-2.0 gram.
5. method as claimed in claim 2, it is characterized in that, the temperature of described cultivation is 25 ~ 37 DEG C; The time of described cultivation is 18 ~ 72 hours.
6. method as claimed in claim 5, it is characterized in that, the temperature of described cultivation is 30 DEG C; The time of described cultivation is 18 hours or 72 hours.
7. method as claimed in claim 2, is characterized in that, described culture condition is that aerobic quiescent culture or concussion are cultivated, and the concussion speed that preferably described concussion is cultivated is 180r/min.
8. method as claimed in claim 2, it is characterized in that, described culture condition is anaerobism quiescent culture.
9. method as claimed in claim 2, it is characterized in that, the step using seed culture medium to carry out seed culture is also comprised before described cultivation, described seed culture medium is TYC substratum, the time of described seed culture is 24 hours, the temperature of described seed culture is 30 DEG C, and the inoculum size of described seed culture is 1%, and described per-cent is volume percent.
10. the application of Leuconostoc mesenteroides CGMCCNo.10064 as claimed in claim 1 in milk-product are produced.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510967496.4A CN105349477A (en) | 2015-12-21 | 2015-12-21 | Leuconostoc mesenteroides as well as preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510967496.4A CN105349477A (en) | 2015-12-21 | 2015-12-21 | Leuconostoc mesenteroides as well as preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105349477A true CN105349477A (en) | 2016-02-24 |
Family
ID=55325542
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510967496.4A Pending CN105349477A (en) | 2015-12-21 | 2015-12-21 | Leuconostoc mesenteroides as well as preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105349477A (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106319081A (en) * | 2016-11-08 | 2017-01-11 | 光明乳业股份有限公司 | Primer and method for identifying leuconostoc mesenteroides subsp.mesenteroides and application of leuconostoc mesenteroides subsp.mesenteroides |
| CN106434588A (en) * | 2016-11-18 | 2017-02-22 | 光明乳业股份有限公司 | GH70 glycosyl transferase and encoding gene and preparation method thereof |
| CN107048203A (en) * | 2017-05-04 | 2017-08-18 | 光明乳业股份有限公司 | A kind of fermented type milk pudding and preparation method thereof |
| CN107080173A (en) * | 2017-05-04 | 2017-08-22 | 光明乳业股份有限公司 | A kind of style milk pudding and preparation method thereof |
| CN108018226A (en) * | 2017-06-24 | 2018-05-11 | 天津大学 | A kind of high-yield extracellular polysaccharide strains and its polysaccharide fermentation production method |
| CN109295127A (en) * | 2018-10-31 | 2019-02-01 | 光明乳业股份有限公司 | A kind of alpha-glucans and the preparation method and application thereof excluding effect with oxygen pressure |
| CN110122578A (en) * | 2019-05-23 | 2019-08-16 | 光明乳业股份有限公司 | A kind of fermented soybean milk powder for being prepared the method for fermented soybean milk powder by Leuconostoc mesenteroides and being prepared and application |
| CN114600966A (en) * | 2022-04-11 | 2022-06-10 | 华中农业大学 | Rice protein yoghourt and preparation method thereof |
| CN118028165A (en) * | 2024-02-29 | 2024-05-14 | 江苏省农业科学院 | Leuconostoc mesenteroides and application thereof |
| CN118028165B (en) * | 2024-02-29 | 2024-07-09 | 江苏省农业科学院 | Leuconostoc mesenteroides and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1692158A (en) * | 2002-08-06 | 2005-11-02 | 丹尼斯科公司 | Use of lactobacillus to produce exopolysaccharides in food and pharmaceutical compositions |
| CN103013891A (en) * | 2012-12-27 | 2013-04-03 | 光明乳业股份有限公司 | Leuconostoc mesenteroides, as well as exopolysaccharide and application thereof |
-
2015
- 2015-12-21 CN CN201510967496.4A patent/CN105349477A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1692158A (en) * | 2002-08-06 | 2005-11-02 | 丹尼斯科公司 | Use of lactobacillus to produce exopolysaccharides in food and pharmaceutical compositions |
| CN103013891A (en) * | 2012-12-27 | 2013-04-03 | 光明乳业股份有限公司 | Leuconostoc mesenteroides, as well as exopolysaccharide and application thereof |
Non-Patent Citations (4)
| Title |
|---|
| BRIAN J.B.WOOD主编，徐岩译: "《发酵食品微生物学》", 31 July 2001 * |
| PRABHU ARCOT PADMANABHAN等: ""Production of insoluble dextran using cell-bound dextransucrase of Leuconostoc mesent eroides NRRL B-523"", 《CARBOHYDRATE RESEARCH》 * |
| RISHIKESH SHUKLA1等: ""Structural Characterization of Insoluble Dextran Produced by Leuconostoc mesenteroides NRRL B-1149 in the Presence of Maltose "", 《FOOD TECHNOL. BIOTECHNOL》 * |
| 鄢明辉等: ""DsrY介导的明串珠菌胞外多糖合成及其在氧损伤应答中的功能研究"", 《第十一次全国基因功能与表观遗传调控学术研讨会摘要集》 * |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106319081A (en) * | 2016-11-08 | 2017-01-11 | 光明乳业股份有限公司 | Primer and method for identifying leuconostoc mesenteroides subsp.mesenteroides and application of leuconostoc mesenteroides subsp.mesenteroides |
| CN106319081B (en) * | 2016-11-08 | 2019-06-21 | 光明乳业股份有限公司 | The primer and its method of identification leuconostoc mesenteroides subsp mesenteroides and application |
| CN106434588A (en) * | 2016-11-18 | 2017-02-22 | 光明乳业股份有限公司 | GH70 glycosyl transferase and encoding gene and preparation method thereof |
| CN107080173A (en) * | 2017-05-04 | 2017-08-22 | 光明乳业股份有限公司 | A kind of style milk pudding and preparation method thereof |
| CN107048203A (en) * | 2017-05-04 | 2017-08-18 | 光明乳业股份有限公司 | A kind of fermented type milk pudding and preparation method thereof |
| CN108018226A (en) * | 2017-06-24 | 2018-05-11 | 天津大学 | A kind of high-yield extracellular polysaccharide strains and its polysaccharide fermentation production method |
| CN109295127A (en) * | 2018-10-31 | 2019-02-01 | 光明乳业股份有限公司 | A kind of alpha-glucans and the preparation method and application thereof excluding effect with oxygen pressure |
| CN109295127B (en) * | 2018-10-31 | 2022-04-01 | 光明乳业股份有限公司 | Alpha-glucan with oxygen pressure exclusion effect and preparation method and application thereof |
| CN110122578A (en) * | 2019-05-23 | 2019-08-16 | 光明乳业股份有限公司 | A kind of fermented soybean milk powder for being prepared the method for fermented soybean milk powder by Leuconostoc mesenteroides and being prepared and application |
| CN114600966A (en) * | 2022-04-11 | 2022-06-10 | 华中农业大学 | Rice protein yoghourt and preparation method thereof |
| CN114600966B (en) * | 2022-04-11 | 2023-09-12 | 华中农业大学 | Rice protein yoghourt and preparation method thereof |
| CN118028165A (en) * | 2024-02-29 | 2024-05-14 | 江苏省农业科学院 | Leuconostoc mesenteroides and application thereof |
| CN118028165B (en) * | 2024-02-29 | 2024-07-09 | 江苏省农业科学院 | Leuconostoc mesenteroides and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105349477A (en) | Leuconostoc mesenteroides as well as preparation method and application thereof | |
| CN102453689B (en) | Lactobacillus plantarum strain producing extracellular polysaccharide, and application thereof | |
| CN102660461B (en) | Microbial preparation for shortening tobacco fermentation period and application of microbial preparation | |
| CN104845912A (en) | Lactobacillus plantarum | |
| CN104694424B (en) | Bacillus amyloliquefaciens strain separated from fermented soybeans and producing protease | |
| Bergey | Thermophilic bacteria | |
| CN110343642A (en) | A kind of preparation method of lactobacillus fermenti and its freeze-dried powder | |
| CN104651268A (en) | Lactobacillus plantarum and application thereof | |
| CN103421704A (en) | Lactobacillus plantarum for freshwater fish fermentation product and application thereof | |
| CN107151638B (en) | Lactobacillus plantarum ZY001 for improving liver function and application thereof in fermented milk | |
| CN102994420A (en) | Low-temperature resistant lactobacillus with ACE (angiotensin converting enzyme) inhibitory activity | |
| CN110093285A (en) | One plant of acidproof lactobacillus fermenti and its application | |
| CN104877940B (en) | One plant of streptococcus thermophilus | |
| CN114437994A (en) | Biological control bacterium Bacillus siamensis HT1 and application thereof in preparation of biological control bacterium agent | |
| CN113502243B (en) | Lactobacillus plantarum GBW-LP001 capable of highly producing lactic acid and antibacterial agent alternative thereof and application | |
| CN102286411B (en) | Lactobacillus plantarum and application thereof in fermenting cabbage wrapper leaf | |
| CN114196584B (en) | Streptomyces avermitilis with low temperature resistance function and application thereof | |
| CN102640886B (en) | Applications of Lactobacillus plantarum in Brassica oleracea outer wrapper leaf silage | |
| CN106282045B (en) | Fermentation medium and fermentation method of Bacillus licheniformis | |
| CN106591176A (en) | Lactobacillus pentosus and application thereof | |
| CN103865832A (en) | Lactobacillus plantarum BS10 and application thereof in regulating pig blood fat | |
| CN105132320A (en) | Culture medium and method for cultivating lactobacillus through high-density solid state fermentation | |
| CN102382779A (en) | Manufacture method for set type additive-free yogurt and lactobacillus casei used therein | |
| CN101886043B (en) | Composite yoghurt starter and application thereof | |
| CN104762295A (en) | Preparation method of acid-resistant salt-resistant facultative anaerobic bacillus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160224 |
|
| RJ01 | Rejection of invention patent application after publication |