CN105343931A - Fibrous membrane for tissue repair and its preparation method, composite fibrous membrane for tissue repair, and their uses - Google Patents
Fibrous membrane for tissue repair and its preparation method, composite fibrous membrane for tissue repair, and their uses Download PDFInfo
- Publication number
- CN105343931A CN105343931A CN201510843761.8A CN201510843761A CN105343931A CN 105343931 A CN105343931 A CN 105343931A CN 201510843761 A CN201510843761 A CN 201510843761A CN 105343931 A CN105343931 A CN 105343931A
- Authority
- CN
- China
- Prior art keywords
- fibrous membrane
- tissue repair
- hot
- membrane
- hydrophobic material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention relates to a fibrous membrane for tissue repair and its preparation method, a composite fibrous membrane for tissue repair, and their uses. The fibrous membrane for tissue repair has a three-dimensional porous structure and has a water absorption in the range of 50 ml/m<2> to 200 ml/2<2>, the fibrous membrane for tissue repair is prepared by hot-pressing a fibrous membrane interwoven of fibers of a hydrophobic material 10 nm to 100 Mum in diameter, and grooves are formed in the surface of the fibrous membrane during hot pressing.
Description
Technical field
The present invention relates to tissue repair fibrous membrane and preparation method thereof, tissue repair composite cellulosic membrane and their application.
Background technology
The hydrophilic of the material of tissue repair fibrous membrane is poor, will be more weak to the absorbability of cell, thus can affect the repair function of tissue repair film.The synthetic material major part being approved for tissue repair film is at present hydrophobic material, so the hydrophilic improving embedded material becomes a focus.In order to promote the adhesion growth of cell on support, mainly adopt the mode of the animal derived materials such as synthetic material composite collagen at present for improving its hydrophilic method of embedded type hydrophobic material, or by chemical modification, grafting water wetted material.Composite animal source property material adds the risk of infection undoubtedly, and chemical modification grafting adds technology difficulty, and relates to the complex work such as the safety evaluatio of the new material of modified generation.
It is as can be seen here, existing that to improve the hydrophilic method of embedded material not ideal enough.
Summary of the invention
the problem that invention will solve
The present invention makes in view of the problem of above-mentioned prior art.The present inventor finds, changes the physical arrangement (fibre morphology) of material although can not change the hydrophobe characteristic of material itself, material can be made to improve absorbent function by physical action, thus cell is more easily attracted on material grow.The present inventor, through intensive research, finds to adopt physical method hydrophobic material to be made into the fibrous membrane had compared with high water absorbing capacity, thus facilitates the adhesion growth of cell on fibrous membrane.
Pass through physical action, the water absorption rate utilizing capillary effect technology to improve hydrophobic material film there is not yet relevant report in medical tissue repair materials, the present inventor carries out hot pressing by the fibrous membrane that hydrophobic material is obtained, make fibrous membrane surface form groove, thus obtain a kind of tissue repair hydrophobic material fibrous membrane with high water absorbing capacity.
Described fibrous membrane surface has groove, and described groove refers to fibrous membrane depression in the surface structure.By by fibrous membrane surface grooves, the surface area of fibrous membrane is increased, compared with the fibrous membrane of flat surface, the area of contact water is more, relends the water absorption rate helping capillary effect and interfibrous gap can improve fibrous membrane.
The object of the invention is to provide a kind of tissue repair fibrous membrane and tissue repair composite cellulosic membrane, and the more common hydrophobic material electrospinning film of this fibrous membrane has higher water absorption rate.
for the scheme of dealing with problems
The invention provides a kind of tissue repair fibrous membrane, described tissue repair fibrous membrane has three-dimensional porous structure, and its water absorption rate is at 50ml/m
2to 200ml/m
2scope in, described tissue repair fibrous membrane is that the fibrous membrane formed by interweaving to the cellosilk of the hydrophobic material being 10nm ~ 100 μm carries out hot-pressing processing and obtains by diameter, in described hot-pressing processing, form groove on fibrous membrane surface.
Preferably, the average pore size of tissue repair fibrous membrane of the present invention is 1 μm to 100 μm.
Preferably, the thickness of tissue repair fibrous membrane of the present invention is 0.1mm ~ 1mm.
Preferably, according to tissue repair fibrous membrane of the present invention, wherein said hydrophobic material be selected from polylactic acid, polycaprolactone, polyvinylidene fluoride, polypropylene, expanded PTFE one or more.
Preferably, described hot-pressing processing is undertaken by being placed in by described fibrous membrane between mould.
Preferably, described hot-pressing processing is undertaken by being placed in by described fibrous membrane between mesh sheet.
The present invention also provides a kind of tissue repair composite cellulosic membrane comprising described tissue repair fibrous membrane, described tissue repair composite cellulosic membrane is compounded to form by two-layer above layer, and wherein at least one deck is the layer formed by tissue repair fibrous membrane of the present invention.
The present invention also provides the preparation method of tissue repair fibrous membrane, specifically comprises the following steps:
(1) prepare fibrous membrane, described fibrous membrane is that the cellosilk of the hydrophobic material of 10nm ~ 100 μm interweaves and formed by diameter;
(2) fibrous membrane prepared in step (1) is carried out hot-pressing processing, obtain described tissue repair fibrous membrane.
Wherein in described step (1), the technology such as electrostatic spinning, centrifugal spinning, hot melt spray webbing, melting electrospinning can be adopted to prepare described fibrous membrane.
Preferably, the method that described step (1) prepares fibrous membrane is electrospinning process, described electrospinning process comprises and is dissolved in solvent by hydrophobic material, obtain the solution of homogeneous hydrophobic material, and the solution of hydrophobic material is loaded in electrostatic spinning syringe, carry out electrostatic spinning, cellosilk is received as membrane structure, obtain fibrous membrane;
In the electrostatic spinning of described step (1), the speed regulating micro-injection pump is 0.1-15.0ml/ hour, and the voltage difference regulating high tension generator is 5-45KV, and the receiving range regulating receiving system is 5.0-30.0cm.
Preferably, in the hot-pressing processing of step (2), be placed between mesh sheet by described fibrous membrane, then in hot press, carry out hot pressing, hot pressing temperature is 45 DEG C ~ 90 DEG C, and hot pressing pressure is 0.4MPa ~ 1.3MPa, and hot pressing time is 3min ~ 10min.
In addition, the present invention also provides the application of described tissue repair fibrous membrane and tissue repair composite cellulosic membrane.
the effect of invention
Compared with prior art, the present invention has following beneficial effect:
Tissue repair fibrous membrane of the present invention is interwoven by the cellosilk of hydrophobic material, has three-dimensional porous structure.More common hydrophobic material fibrous membrane has higher water absorption rate, and water absorption rate is at 50ml/m
2to 200ml/m
2scope in.This hydrophobic material fibrous membrane surface has groove.This fibrous membrane surface groovesization can improve the water absorption rate of hydrophobic material film.
Accompanying drawing explanation
Fig. 1 is the scanning electron microscope (SEM) photograph of the tissue repair fibrous membrane that embodiment 1 obtains.
Detailed description of the invention
One aspect of the present invention relates to tissue repair fibrous membrane and preparation method thereof, relates to tissue repair fibrous membrane and the application of tissue repair composite cellulosic membrane in soft tissue repair on the other hand.
I, tissue repair fibrous membrane of the present invention
Tissue repair fibrous membrane of the present invention has three-dimensional porous structure, and the water absorption rate of described fibrous membrane is at 50ml/m
2to 200ml/m
2scope in, described tissue repair fibrous membrane is that the fibrous membrane formed by interweaving to the cellosilk of the hydrophobic material being 10nm ~ 100 μm carries out hot-pressing processing and obtains by diameter, in described hot-pressing processing, form groove on fibrous membrane surface, described groove refers to fibrous membrane depression in the surface structure.The filametntary diameter of described hydrophobic material is preferably 500nm ~ 5 μm, and the water absorption rate of described tissue repair fibrous membrane is preferably at 100ml/m
2to 150ml/m
2scope in.
Preferably, the average pore size of described tissue repair fibrous membrane is 1 μm ~ 100 μm, more preferably 2 μm ~ 10 μm.Preferably, the thickness of described tissue repair fibrous membrane is 0.1mm ~ 1mm, more preferably 0.1mm ~ 0.3mm.
In the present invention, the filametntary diameter of hydrophobic material is undertaken measuring by the method for scanning electron microscope; The average pore size of described tissue repair fibrous membrane is with reference to ASTMD6767-2002, utilizes Capillary Flow average pore size analyser to be undertaken measuring by Vesicular protein; The thickness of described tissue repair fibrous membrane is measured according to GB/T7689.1-2001 method by compressibility Fabric Style instrument; The water absorption rate of described tissue repair fibrous membrane is measured by ASTMD6651-01 method, and the water absorption rate described in the present invention is the external absorbability in ASTMD6651-01.
Described fibrous membrane has groove, described groove refers to fibrous membrane depression in the surface structure, the structure marked as shown by the arrows in Figure 1, and the structure marked is only exemplary, and be not limited only to the place that is marked in Fig. 1, also comprise the structure with the similar marked.The existence of groove makes the surface area of fibrous membrane increase, and compared with the fibrous membrane of flat surface, the area that the fluted fibrous membrane of surperficial tool contacts with water increases, and relends the water absorption rate helping capillary effect and interfibrous gap can improve fibrous membrane.
Preferably, described hydrophobic material be selected from polylactic acid (PLA), polycaprolactone (PCL), polyvinylidene fluoride (PVDF), polypropylene (PP), expanded PTFE (PTFE) one or more, preferred, described hydrophobic material is polylactic acid (PLA), and further preferred described hydrophobic material is PLLA (PLLA).
Described hot-pressing processing can adopt mould to carry out under certain temperature and pressure condition.Described mould is thermoplastic braiding mesh sheet or metal knitted mesh sheet, or surface has the metal die of the lines alternately formed by depression and strip bulge.
Described hot-pressing processing is carried out as follows: be placed in by described fibrous membrane between described mould, then in hot press, carry out hot pressing, and hot pressing temperature is 45 DEG C ~ 90 DEG C, and hot pressing pressure is 0.4MPa ~ 1.3MPa, and hot pressing time is 3min ~ 10min.Hot pressing temperature more preferably 50 DEG C ~ 70 DEG C, preferred, hot pressing pressure is 0.6MPa ~ 0.8MPa, and hot pressing time is 4min ~ 7min.Preferably, described mould is thermoplastic braiding mesh sheet, and the material of described braiding mesh sheet is one or more in polypropylene, polyvinylidene fluoride, expanded PTFE.The hole of preferred described braiding mesh sheet is 0.25mm
2~ 2mm
2, fibre diameter is 0.05mm ~ 0.1mm.
Braiding mesh sheet mainly plays the effect of mould, because the hardness of the silk thread of mesh sheet is greater than the hardness of fibrous membrane during hot pressing, makes fibrous membrane is produced groove by hot pressing.After hot pressing terminates, described braiding mesh sheet is removed.Preferably first that described fibrous membrane is swelling in ethanol water, and then the fibrous membrane after swelling is placed between mesh sheet carries out hot pressing.Degree of swelling suitably can regulate according to adopted braiding mesh sheet.
The preparation method of II, tissue repair fibrous membrane
As the manufacture method of tissue repair fibrous membrane of the present invention, specifically comprise the steps:
(1) prepare fibrous membrane, described fibrous membrane is that the cellosilk of the hydrophobic material of 10nm ~ 100 μm interweaves and formed by diameter;
(2) fibrous membrane prepared in step (1) is carried out hot-pressing processing, obtain tissue repair fibrous membrane.
Wherein in described step (1), electrospinning process, centrifugal spinning method, hot melt silk spraying method or melting electrospinning method can be adopted to prepare described fibrous membrane.
Preferably, in described step (1), electrospinning process is adopted to prepare described fibrous membrane.Described electrospinning process comprises and is dissolved in solvent by hydrophobic material, obtains the solution of homogeneous hydrophobic material, and is loaded in electrostatic spinning syringe by the solution of described hydrophobic material, carries out electrostatic spinning, cellosilk is received as membrane structure, obtain fibrous membrane.In addition, two or more different hydrophobic material also can be used to carry out electrostatic spinning to prepare fibrous membrane.
For the concentration of electrospun solution, suitably can regulate according to the type of the kind of hydrophobic material and solvent, and without particular limitation of.
In the electrostatic spinning of above-mentioned steps (1), the speed preferably regulating micro-injection pump is 0.1-15.0ml/ hour, more preferably 3.0 ~ 8.0ml/ hour.
In the electrostatic spinning of above-mentioned steps (1), the voltage difference preferably regulating high tension generator is 5-45KV, more preferably 15 ~ 30KV.
In the electrostatic spinning of above-mentioned steps (1), the receiving range preferably regulating receiving system is 5.0-30.0cm, more preferably 15.0 ~ 25.0cm.
Described hot-pressing processing can adopt mould to carry out under certain temperature and pressure condition.Described mould is thermoplastic braiding mesh sheet or metal knitted mesh sheet, or surface has the metal die of the lines alternately formed by depression and strip bulge.Preferably, described mould is thermoplastic braiding mesh sheet.
Preferably, in the hot-pressing processing of step (2), described fibrous membrane is placed between two thermoplastic braiding mesh sheet, then in hot press, carries out hot pressing.Regulate hot pressing temperature to be 45 DEG C ~ 90 DEG C, more preferably 50 DEG C ~ 70 DEG C, adjustment hot pressing pressure is 0.4MPa ~ 1.3MPa, more preferably 0.6MPa ~ 0.8MPa, and adjustment hot pressing time is 3min ~ 10min, more preferably 4min ~ 7min.Preferably, the material of described thermoplasticity braiding mesh sheet is one or more in polypropylene (PP), polyvinylidene fluoride (PVDF), expanded PTFE (PTFE).The hole of preferred described braiding mesh sheet is 0.25mm
2~ 2mm
2, fibre diameter is 0.05mm ~ 0.1mm.
Braiding mesh sheet plays the effect of mould, because the hardness of the silk thread of mesh sheet is greater than the hardness of fibrous membrane during hot pressing, makes fibrous membrane surface be produced groove by hot pressing.After hot pressing terminates, described braiding mesh sheet is removed.Preferably first that described fibrous membrane is swelling in ethanol water, and then the fibrous membrane after swelling is placed between mesh sheet carries out hot pressing.Degree of swelling suitably can regulate according to adopted braiding mesh sheet.
In above-mentioned steps (2), be preferably 15% ~ 95% (volumetric concentration by the fibrous membrane prepared in step (1) in concentration, v/v) swelling in ethanol water, more preferably ethanol water concentration is 75% ~ 95% (volumetric concentration, v/v).
Tissue repair fibrous membrane of the present invention can further with at least one deck other layer and/or tissue repair fibrous membrane compound in addition more than one deck thus obtain tissue repair composite cellulosic membrane.Other described layer can be the braiding mesh sheet such as polyvinylidene fluoride (PVDF), polypropylene (PP), expanded PTFE (PTFE), polyethylene terephthalate (PET), also can be the anti such as chitosan, hyaluronic acid type coating; The complex method preparing composite cellulosic membrane can be complex method of the prior art, such as hot pressing, stitching, bonding, spraying etc.
The application > of < tissue repair fibrous membrane and tissue repair composite cellulosic membrane
The present invention also provides described tissue repair fibrous membrane and the application of tissue repair composite cellulosic membrane in soft tissue repair goods, and described soft tissue repair goods include but not limited to that artificial skin, hernia repair goods, tooth film healing goods, cerebral dura mater repair goods, spinal meninges repairs goods.
The present invention is further described below in conjunction with specific embodiment.The reagent that the embodiment of the present invention adopts and raw material unless stated otherwise, are all commercial conventional products.
embodiment
The preparation > of < tissue repair fibrous membrane
Embodiment 1
(1) be dissolved in hexafluoroisopropanol solvent by PLLA, obtain uniform macromolecular solution, wherein the quality concentration of volume percent of PLLA is 8g/100ml;
This macromolecular solution is loaded in electrostatic spinning syringe, the speed regulating micro-injection pump is 10ml/ hour, the voltage difference regulating high tension generator is 22KV, the receiving range regulating receiving system is 18cm, the fibrous membrane of crisscross intersection is prepared by electrostatic spinning, after fibrous membrane thickness reaches 0.3mm, close electrostatic spinning, take off fibrous membrane;
(2) fibrous membrane obtained in (1) being put in concentration is 95% (volumetric concentration, v/v) swelling in ethanol water, (the PPM5 model braiding mesh sheet of BiomedicalStructures company, hole is 0.25mm respectively to cover one deck PP braiding mesh sheet in the upper and lower surface of described fibrous membrane after swelling
2~ 2mm
2, wire diameter is 0.1mm), be then placed in hot press, temperature be 55 DEG C, pressure be 0.8MPa condition under hot pressing 3min.After hot pressing terminates, braiding mesh sheet is taken off, obtain the fibrous membrane that fibrous membrane surface has many grooves.As shown in Figure 1, more groove is defined on fibrous membrane surface by hot pressing as seen.
In embodiment 1, the fibrous membrane of step (1) gained cannot be flooded profit in water, does not absorb water; The average fibre diameter of the tissue repair fibrous membrane in step (2) after hot-pressing processing is 1.2 μm, and film thickness is 0.19mm, and water absorption rate is 132ml/m
2.Visible, the present invention, by hydrophobic fibre film is carried out hot-pressing processing, makes fibrous membrane surface have groove structure, can improve the water absorption rate of hydrophobic fibre film well.
Embodiment 2
(1) be dissolved in hexafluoroisopropanol solvent by PCL, obtain uniform macromolecular solution, wherein the quality concentration of volume percent of PCL is 12g/100ml;
This macromolecular solution is loaded in electrostatic spinning syringe, the speed regulating micro-injection pump is 6ml/ hour, the voltage difference regulating high tension generator is 20KV, the receiving range regulating receiving system is 18cm, the fibrous membrane of crisscross intersection is prepared by electrostatic spinning, after fibrous membrane thickness reaches 0.3mm, close electrostatic spinning, take off fibrous membrane;
(2) (the PPM5 model braiding mesh sheet of BiomedicalStructures company, hole is 0.25mm the upper and lower surface of fibrous membrane obtained in (1) respectively to be covered one deck PP braiding mesh sheet
2~ 2mm
2, filament diameter is 0.1mm), being then placed in hot press in temperature is 45 DEG C, and pressure is hot pressing 3min under 0.8MPa condition, after hot pressing terminates, braiding mesh sheet is taken off, and obtains the fibrous membrane that fibrous membrane surface has many grooves.
In embodiment 2, the fibrous membrane of step (1) gained cannot be flooded profit in water, does not absorb water; The average fibre diameter of the gained tissue repair fibrous membrane in step (2) after hot-pressing processing is 1.0 μm, and film thickness is 0.15mm, and water absorption rate is 93ml/m
2.Visible, the present invention, by hydrophobic fibre film is carried out hot-pressing processing, makes fibrous membrane surface have groove structure, can improve the water absorption rate of hydrophobic fibre film well.
Embodiment 3
(1) be dissolved in hexafluoroisopropanol solvent by PLLA, obtain uniform macromolecular solution, wherein the quality concentration of volume percent of PLLA is 8g/100ml;
Be dissolved in by PVDF in the mixed solvent of DMF and acetone, in described mixed solvent, the volume ratio of DMF and acetone is 2:3, obtains uniform macromolecular solution again, and wherein the quality concentration of volume percent of PVDF is 18g/100ml;
Above-mentioned PLLA solution and PVDF solution are respectively charged in electrostatic spinning syringe, the speed regulating micro-injection pump is 8ml/ hour, the voltage difference regulating high tension generator is 22KV, the receiving range regulating receiving system is 22cm, the fibrous membrane of crisscross intersection is prepared by electrostatic spinning, close electrostatic spinning after reaching the thickness of 0.3mm, take off fibrous membrane.
(2) fibrous membrane obtained in (1) being put in concentration is 95% (volumetric concentration, v/v) swelling in ethanol water, (the PPM5 model braiding mesh sheet of BiomedicalStructures company, hole is 0.25mm respectively to cover one deck PP braiding mesh sheet in the upper and lower surface of described fibrous membrane after swelling
2~ 2mm
2, filament diameter is 0.1mm), being then placed in hot press in temperature is 70 DEG C, and pressure is hot pressing 5min under 1.0MPa condition, after hot pressing terminates, braiding mesh sheet is taken off, and obtains the fibrous membrane that fibrous membrane surface has many grooves.
In embodiment 3, the fibrous membrane of step (1) gained cannot be flooded profit in water, does not absorb water; The average fibre diameter of the tissue repair fibrous membrane of the gained in step (2) after hot-pressing processing is 1.6 μm, and film thickness is 0.23mm, and water absorption rate is 114ml/m
2.Visible, the present invention, by hydrophobic fibre film is carried out hot-pressing processing, makes fibrous membrane surface have groove structure, can improve the water absorption rate of hydrophobic fibre film well.
Embodiment 4
(1) be dissolved in hexafluoroisopropanol solvent by PLLA, obtain uniform macromolecular solution, wherein the quality concentration of volume percent of PLLA is 8g/100ml;
Loaded by this macromolecular solution in centrifugal force spinning-drawing machine raw material storage tank, open centrifugal force spinning-drawing machine, arranging rotating speed is 3500 revs/min, namely stopping, obtaining fluffy fibrous membrane when having spun 100ml solution.
(2) fibrous membrane obtained in (1) being put in concentration is 95% (volumetric concentration, v/v) swelling in ethanol water, (the PPM5 model braiding mesh sheet of BiomedicalStructures company, hole is 0.25mm respectively to cover one deck PP braiding mesh sheet in the upper and lower surface of described fibrous membrane after swelling
2~ 2mm
2, filament diameter is 0.1mm), being then placed in hot press in temperature is 70 DEG C, and pressure is hot pressing 5min under 1.0MPa condition, after hot pressing terminates, braiding mesh sheet is taken off, and obtains the fibrous membrane that fibrous membrane surface has many grooves.
In embodiment 4, the fibrous membrane of step (1) gained cannot be flooded profit in water, does not absorb water; The average fibre diameter of the tissue repair fibrous membrane of the gained in step (2) after hot-pressing processing is 2.3 μm, and film thickness is 0.21mm, and water absorption rate is 155ml/m
2.Visible, the present invention, by hydrophobic fibre film is carried out hot-pressing processing, makes fibrous membrane surface have groove structure, can improve the water absorption rate of hydrophobic fibre film well.
Embodiment 5
(1) loaded in centrifugal force spinning-drawing machine raw material storage tank by PCL granule, open centrifugal force spinning-drawing machine, arranging heating-up temperature is 120 DEG C, and rotating speed is 3000 revs/min, namely stopping, obtaining fluffy fibrous membrane when having spun 12gPCL.
(2) (the PPM5 model braiding mesh sheet of BiomedicalStructures company, hole is 0.25mm the upper and lower surface of fibrous membrane obtained in (1) respectively to be covered one deck PP braiding mesh sheet
2~ 2mm
2, filament diameter is 0.1mm), being then placed in hot press in temperature is 45 DEG C, and pressure is hot pressing 5min under 1.0MPa condition, after hot pressing terminates, braiding mesh sheet is taken off, and obtains the fibrous membrane that fibrous membrane surface has many grooves.
In embodiment 5, the fibrous membrane of step (1) gained cannot be flooded profit in water, does not absorb water; The average fibre diameter of the tissue repair fibrous membrane of the gained in step (2) after hot-pressing processing is 2.6 μm, and film thickness is 0.23mm, and water absorption rate is 116ml/m
2.Visible, the present invention, by hydrophobic fibre film is carried out hot-pressing processing, makes fibrous membrane surface have groove structure, can improve the water absorption rate of hydrophobic fibre film well.
Embodiment 6
(1) be dissolved in hexafluoroisopropanol solvent by PLLA, obtain uniform macromolecular solution, wherein the quality concentration of volume percent of PLLA is 6g/100ml;
This macromolecular solution is loaded in electrostatic spinning syringe, the speed regulating micro-injection pump is 6ml/ hour, the voltage difference regulating high tension generator is 22KV, the receiving range regulating receiving system is 18cm, the fibrous membrane of crisscross intersection is prepared by electrostatic spinning, after fibrous membrane thickness reaches 0.3mm, close electrostatic spinning, take off fibrous membrane;
(2) fibrous membrane obtained in (1) being put in concentration is 95% (volumetric concentration, v/v) swelling in ethanol water, (the PPM5 model braiding mesh sheet of BiomedicalStructures company, hole is 0.25mm respectively to cover one deck PP braiding mesh sheet in the upper and lower surface of described fibrous membrane after swelling
2~ 2mm
2, wire diameter is 0.1mm), be then placed in hot press, temperature be 55 DEG C, pressure be 1.0MPa condition under hot pressing 3min.After hot pressing terminates, braiding mesh sheet is taken off, obtain the fibrous membrane that fibrous membrane surface has many grooves.
(3) on a face of the fibrous membrane of step (2) gained, place one deck PPM5 weave mesh sheet, fold rear suture and sew up, obtain the composite cellulosic membrane that PLLA and PP weaves mesh sheet, to reach the object strengthening membrane ruggedness.
Tissue repair fibrous membrane of the present invention and tissue repair composite cellulosic membrane may be used for soft tissue repair goods, and such as artificial skin, hernia repair goods, tooth film healing goods, cerebral dura mater repair goods, spinal meninges reparation goods etc.Tissue repair fibrous membrane of the present invention and tissue repair composite cellulosic membrane are owing to having higher water absorption rate, more soft after immersion, therefore strengthen with the attaching ability of tissue after implant into body, promote the adhesion growth of cell on fibrous membrane, be conducive to the reparation, the regeneration that promote tissue, and when implanting in operation, the frictional force between diaphragm and tissue can be reduced after diaphragm immersion, operate easier (such as more easily putting into when preperitoneal space implant procedure) etc.
The application of tissue repair fibrous membrane in stomach wall is repaired
The fibrous membrane (fibrous membrane namely before hot pressing and after hot pressing) step (1) in embodiment 1 and step (2) obtained respectively carries out cutting, cleaning, sterilizing, make matched group soft tissue repair sticking patch and (be called for short matched group, electrospun fiber membrane for common in prior art) and experimental group soft tissue repair sticking patch (abbreviation experimental group, for tissue repair fibrous membrane of the present invention), carry out animal abdominal wall and cut damage reparative experiment.Before zoopery, a slice diaphragm is got respectively from matched group and experimental group, after diaphragm be immersed in the water make it be flooded profit as far as possible with identical operation, feel the softness of two kinds of diaphragms by feel, result experimental group soft tissue repair sticking patch (i.e. diaphragm after hot-pressing processing of the present invention) is obviously more soft.
Select healthy new zealand rabbit 12, male and female are not limit, and are divided into two groups, often organize 6.The new zealand rabbit entering experiment is anaesthetized, implements preserved skin, lying on the back is bound on plank; After sterilization paving is single, hit exactly in abdominal part, skin is cut along rabbit hunter's line with 4# scalpel, being about 8cm, exposing muscle, is stomach wall holostrome (comprising peritoneum, muscle, the fascia tissue) excision of 2cm × 5cm by size, artificial fabrication portion abdominal-wall defect, then matched group soft tissue repair sticking patch or experimental group soft tissue repair sticking patch are immersed in the water after making it be flooded profit as far as possible, defect kposthesis is implemented to rabbit, matched group and experimental group parallel laboratory test 6 rabbits respectively.Postoperative nursing and observation rabbit being carried out to routine.
Respectively at postoperative 3 months, 6 months, 12 months, random selecting two rabbits in matched group and experimental group respectively, centered by the operative site of animal, cut specimen being greater than operative site 1cm scope, make it comprise soft tissue repair sticking patch and periphery abdominal wall tissue, carry out paired observation.Observed result is as shown in table 1:
Table 1 matched group and the experimental group reparation situation within different repair time compares
Results of animal shows, the repairing effect that experimental group soft tissue repair sticking patch cuts damage to stomach wall is better.Absolutely prove and the adhesion growth that tissue repair fibrous membrane of the present invention can promote cell on fibrous membrane be conducive to the reparation, the regeneration that promote tissue.
The above; be only the specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, is anyly familiar with those skilled in the art in the technical scope that the present invention discloses; change can be expected easily or replace, all should be encompassed within protection scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with the protection domain of described claim.
Claims (13)
1. a tissue repair fibrous membrane, is characterized in that, described tissue repair fibrous membrane has three-dimensional porous structure, and its water absorption rate is at 50ml/m
2to 200ml/m
2scope in, described tissue repair fibrous membrane is that the fibrous membrane formed by interweaving to the cellosilk of the hydrophobic material being 10nm ~ 100 μm carries out hot-pressing processing and obtains by diameter, in described hot-pressing processing, form groove on fibrous membrane surface.
2. tissue repair fibrous membrane according to claim 1, is characterized in that, the average pore size of described tissue repair fibrous membrane is 1 μm to 100 μm.
3. tissue repair fibrous membrane according to claim 1 and 2, is characterized in that, the thickness of described tissue repair fibrous membrane is 0.1mm ~ 1mm.
4. the tissue repair fibrous membrane according to any one of claim 1-3, is characterized in that, described hydrophobic material be selected from polylactic acid, polycaprolactone, polyvinylidene fluoride, polypropylene, expanded PTFE one or more.
5. the tissue repair fibrous membrane according to any one of claim 1-4, is characterized in that, described hot-pressing processing is undertaken by being placed in by described fibrous membrane between mould.
6. the tissue repair fibrous membrane according to any one of claim 1-5, is characterized in that, described hot-pressing processing is undertaken by being placed in by described fibrous membrane between mesh sheet.
7. a tissue repair composite cellulosic membrane, is characterized in that, described tissue repair composite cellulosic membrane is compounded to form by two-layer above layer, wherein at least layer of one deck for being formed by the tissue repair fibrous membrane described in any one of claim 1-6.
8. a preparation method for the tissue repair fibrous membrane according to any one of claim 1-6, is characterized in that, comprise the following steps:
(1) prepare fibrous membrane, described fibrous membrane is that the cellosilk of the hydrophobic material of 10nm ~ 100 μm interweaves and formed by diameter;
(2) fibrous membrane prepared in step (1) is carried out hot-pressing processing, obtain described tissue repair fibrous membrane.
9. the preparation method of tissue repair fibrous membrane according to claim 8, it is characterized in that, prepare described fibrous membrane by electrospinning process in step (1), described electrospinning process comprises and is dissolved in solvent by hydrophobic material, obtain the solution of homogeneous hydrophobic material, and the solution of hydrophobic material is loaded in electrostatic spinning syringe, carry out electrostatic spinning, cellosilk is received as membrane structure, obtains fibrous membrane.
10. the preparation method of tissue repair fibrous membrane according to claim 9, it is characterized in that, in the electrostatic spinning of step (1), the speed regulating micro-injection pump is 0.1-15.0ml/ hour, the voltage difference regulating high tension generator is 5-45KV, and the receiving range regulating receiving system is 5.0-30.0cm.
11. preparation methoies according to Claim 8 according to any one of-10, it is characterized in that, in the hot-pressing processing of step (2), described fibrous membrane is placed between mesh sheet, then in hot press, hot pressing is carried out, hot pressing temperature is 45 DEG C ~ 90 DEG C, and hot pressing pressure is 0.4MPa ~ 1.3MPa, and hot pressing time is 3min ~ 10min.
Tissue repair fibrous membrane described in 12. any one of claim 1-6 or the application of tissue repair composite cellulosic membrane according to claim 7 in soft tissue repair goods.
13. application according to claim 12, is characterized in that, described soft tissue repair goods comprise artificial skin, hernia repair goods, tooth film healing goods, cerebral dura mater repairs goods or spinal meninges repairs goods.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510843761.8A CN105343931B (en) | 2015-11-27 | 2015-11-27 | Tissue repair tunica fibrosa and preparation method thereof, tissue repair composite cellulosic membrane and their application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510843761.8A CN105343931B (en) | 2015-11-27 | 2015-11-27 | Tissue repair tunica fibrosa and preparation method thereof, tissue repair composite cellulosic membrane and their application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105343931A true CN105343931A (en) | 2016-02-24 |
| CN105343931B CN105343931B (en) | 2018-04-20 |
Family
ID=55320084
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510843761.8A Active CN105343931B (en) | 2015-11-27 | 2015-11-27 | Tissue repair tunica fibrosa and preparation method thereof, tissue repair composite cellulosic membrane and their application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105343931B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106726006A (en) * | 2016-12-28 | 2017-05-31 | 广州迈普再生医学科技有限公司 | Bionical ligament and bionical ligamentary system |
| CN107254805A (en) * | 2017-06-09 | 2017-10-17 | 浙江大学 | A kind of hydrophobically modified method of paper |
| CN108057132A (en) * | 2018-01-26 | 2018-05-22 | 浙江大学 | A kind of striping polylactide-caprolactone film of surface retention gelatin and preparation method thereof |
| CN114177364A (en) * | 2021-12-17 | 2022-03-15 | 无锡中科光远生物材料有限公司 | Soft tissue enhancement film material and preparation method and application thereof |
| CN114887119A (en) * | 2022-05-09 | 2022-08-12 | 浙江大学医学院附属第一医院 | High-molecular artificial dura mater with anti-infection capacity and preparation method thereof |
| TWI787744B (en) * | 2020-03-02 | 2022-12-21 | 美商Ｅｌｃ管理公司 | Delivery of cosmetic agents, compositions and use thereof |
| AU2020330857B2 (en) * | 2019-08-12 | 2023-07-13 | Corneat Vision Ltd. | Gingival graft |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101507661A (en) * | 2009-03-10 | 2009-08-19 | 广州迈普再生医学科技有限公司 | Nano artificial dura mater with multi functional-layers and preparation method thereof |
| CN102358067A (en) * | 2011-07-27 | 2012-02-22 | 江南大学 | Super-hydrophobic textile and processing method thereof |
| WO2012135593A1 (en) * | 2011-03-30 | 2012-10-04 | Ethicon, Inc. | Tissue repair devices of rapid therapeutic absorbency |
| CN102965849A (en) * | 2012-11-22 | 2013-03-13 | 天津大学 | Method for preparing medical barrier membrane by electrostatic spinning |
| CN104645420A (en) * | 2014-12-29 | 2015-05-27 | 深圳迈普再生医学科技有限公司 | Soft tissue repairing patch and preparation method thereof |
-
2015
- 2015-11-27 CN CN201510843761.8A patent/CN105343931B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101507661A (en) * | 2009-03-10 | 2009-08-19 | 广州迈普再生医学科技有限公司 | Nano artificial dura mater with multi functional-layers and preparation method thereof |
| WO2012135593A1 (en) * | 2011-03-30 | 2012-10-04 | Ethicon, Inc. | Tissue repair devices of rapid therapeutic absorbency |
| CN102358067A (en) * | 2011-07-27 | 2012-02-22 | 江南大学 | Super-hydrophobic textile and processing method thereof |
| CN102965849A (en) * | 2012-11-22 | 2013-03-13 | 天津大学 | Method for preparing medical barrier membrane by electrostatic spinning |
| CN104645420A (en) * | 2014-12-29 | 2015-05-27 | 深圳迈普再生医学科技有限公司 | Soft tissue repairing patch and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 武秀阁等: "全氟磺酸中空纤维膜的成形工艺研究（Ⅱ）", 《合成纤维工艺》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106726006A (en) * | 2016-12-28 | 2017-05-31 | 广州迈普再生医学科技有限公司 | Bionical ligament and bionical ligamentary system |
| CN107254805A (en) * | 2017-06-09 | 2017-10-17 | 浙江大学 | A kind of hydrophobically modified method of paper |
| CN108057132A (en) * | 2018-01-26 | 2018-05-22 | 浙江大学 | A kind of striping polylactide-caprolactone film of surface retention gelatin and preparation method thereof |
| AU2020330857B2 (en) * | 2019-08-12 | 2023-07-13 | Corneat Vision Ltd. | Gingival graft |
| TWI787744B (en) * | 2020-03-02 | 2022-12-21 | 美商Ｅｌｃ管理公司 | Delivery of cosmetic agents, compositions and use thereof |
| CN114177364A (en) * | 2021-12-17 | 2022-03-15 | 无锡中科光远生物材料有限公司 | Soft tissue enhancement film material and preparation method and application thereof |
| CN114887119A (en) * | 2022-05-09 | 2022-08-12 | 浙江大学医学院附属第一医院 | High-molecular artificial dura mater with anti-infection capacity and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105343931B (en) | 2018-04-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105343931A (en) | Fibrous membrane for tissue repair and its preparation method, composite fibrous membrane for tissue repair, and their uses | |
| CN101214393B (en) | Nano fibrous tissue engineering blood vessel and preparation thereof | |
| WO2017088818A1 (en) | Tissue repair fiber membrane, preparation method and application thereof, and tissue repair product | |
| Nitti et al. | Influence of nanofiber orientation on morphological and mechanical properties of electrospun chitosan mats | |
| CN106492272B (en) | Tissue repair tunica fibrosa and its preparation method and application and tissue repair product | |
| CN105194737B (en) | A kind of tissue recovery support and its preparation method and application | |
| EP3015120B1 (en) | Tissue repair scaffold and preparation method and purpose thereof | |
| CN107073173B (en) | Method for producing a hybrid structure consisting of combined silk fibroin micro-and nanofibers, hybrid structure thus obtained and use thereof as implantable medical device | |
| Lim et al. | Fabrication and evaluation of poly (epsilon‐caprolactone)/silk fibroin blend nanofibrous scaffold | |
| CN104414773A (en) | Anti-adhesion tissue repair membrane and preparation method thereof | |
| EP2465472B1 (en) | Method for producing a silk nanofiber nerve conduit | |
| CN100493626C (en) | Cell culturing rack material and its preparation | |
| CN104168856B (en) | Replace the improved reinforcing PGA absorbability sticking patch of a wall of urinary bladder part after partial cystectomy | |
| CN101507843A (en) | Multi-purpose surgery biology patching material | |
| Thomas et al. | Electrospinning of Biosyn®-based tubular conduits: Structural, morphological, and mechanical characterizations | |
| CN108478866A (en) | Tissue recovery support, preparation method and use | |
| McCullen et al. | Fiber-reinforced scaffolds for tissue engineering and regenerative medicine: use of traditional textile substrates to nanofibrous arrays | |
| CN109806440A (en) | Mitigate foreign body reaction and prevents composite polypropylene sticking patch of adhesion and preparation method thereof | |
| CN106535950B (en) | Dental regenerated film using silkworm cocoon and preparation method thereof | |
| JP4583927B2 (en) | Tissue support prosthesis | |
| Serafini et al. | The patenting and technological trends in hernia mesh implants | |
| KR102233499B1 (en) | 3-Dimensional fibrous scaffold | |
| Fattahi | Emerging Nanofibrous Polycaprolactone Vascular Grafts in Small and Large Animal Models: in vivo and in vitro Analyses | |
| CN104721886A (en) | Tissue repair membrane with fiber structure, preparation method, using method and application thereof | |
| Nitti et al. | Research Article Influence of Nanofiber Orientation on Morphological and Mechanical Properties of Electrospun Chitosan Mats |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 510633 Third Floor of Zone E, No. 80 Luanyue Road, Science City, Guangzhou High-tech Industrial Development Zone, Guangzhou, Guangdong Province Patentee after: Guangzhou Maple regenerative medicine Polytron Technologies Inc Address before: 510633 Third Floor of Zone E, No. 80 Luanyue Road, Science City, Guangzhou High-tech Industrial Development Zone, Guangzhou, Guangdong Province Patentee before: Medprin Regenerative Medical Technologies Co., Ltd. |
|
| CP01 | Change in the name or title of a patent holder |