CN105339042A

CN105339042A - Phototherapeutic device, method and use

Info

Publication number: CN105339042A
Application number: CN201480011126.7A
Authority: CN
Inventors: N·卢皮斯; R·皮耶尔加利尼
Original assignee: Klox Technologies Inc
Current assignee: Klox Technologies Inc
Priority date: 2013-03-01
Filing date: 2014-02-28
Publication date: 2016-02-17
Also published as: HK1219452A1; EP2961478A4; EP2961478A1; HK1219689A1

Abstract

Disclosed herein are devices, systems, and methods for providing light therapy to a subject's tissues. The devices, systems, and methods include a phototherapeutic lamp comprising light generating sources that emit light capable of causing a medical and/or cosmetic treatment of tissues. Also included are uses of the devices. The devices and methods may also include a photoactivatable composition.

Description

Phototherapy device, method and purposes

Background technology

Phototherapy relates to employing electromagnetic radiation (such as visible ray and infrared light) and processes biological tissue.Phototherapy is widely used in medical science and beauty treatment fields, comprises skin renewal and the various dermatosis for the treatment of.Phototherapy also uses together with some photosensitive drug or photoactivatable composition.Need to provide a kind of novel phototherapy device and the method with beauty treatment or medical usage.

Summary of the invention

From broadly, the invention provides the purposes of a kind of phototherapy device, a kind of method and described device, comprise and adopt the light with different and complementary therapeutical effect to process the different situations of experimenter, experimenter can be human or animal.

From broadly, the different aspect of described device extensively comprises at least one light source, described light source launches the emission spectra with one or more characteristics following: (i) produces antibacterial effect in the light-struck treatment sites of described transmitting, (ii) blood flow for the treatment of sites is regulated, and/or (iii) activation comprise at least one chromophore/fluorescent dye can photoactivatable composition, described can photoactivatable composition can launch after activation have treatment characteristic light (fluorescence or phosphorescence), treatment characteristic such as regulates the blood flow for the treatment of sites, regulate collagen protein and/or above therapentic part or the reactivity kind that therapentic part is inner or near therapentic part, release has biology-therapeutic effect, such as singlet oxygen.

On the one hand, the invention provides a kind of phototherapy device, comprise the first light source and secondary light source, first light source can launch the first light, the emission spectra of the first light can activate be applied in above therapentic part or near therapentic part can photoactivatable composition, secondary light source can launch the second light, and the emission spectra of the second light is different from the first light, and wherein the emission spectra of the first light and the emission spectra of the second light are in blue light region and/or the purple light district of electromagnetic spectrum.In certain embodiments, the first light is in the purple light district of electromagnetic spectrum, and the second light is in the blue light region of electromagnetic spectrum.

On the other hand, the invention provides a kind of phototherapy device, comprising: the first light source and secondary light source, the first light source can launch the first light with the first emission spectra; Secondary light source can launch the second light with the second emission spectra, and the second emission spectra is different from the first emission spectra, and wherein the first emission spectra and the second emission spectra are in blue light region and/or the purple light district of electromagnetic spectrum.In certain embodiments, the first light is in the blue light region of electromagnetic spectrum, and the second light is in the purple light district of electromagnetic spectrum.

On the other hand, the invention provides a kind of phototherapy device, comprise: the first light source and secondary light source, first light source can launch the first light with the first peak wavelength, for activate be applied in above therapentic part or near therapentic part can photoactivatable composition, secondary light source can launch the second light with the second peak wavelength, and the second peak wavelength is different from the first peak wavelength, and wherein the first peak wavelength and the second peak wavelength are in blue light region and/or the purple light district of electromagnetic spectrum.

On the other hand, the invention provides a kind of phototherapy device, comprise: the first light source and secondary light source, first light source can launch the first light with the first peak wavelength, secondary light source can launch the second light with the second peak wavelength, second peak wavelength is different from the first peak wavelength, and wherein the first peak wavelength is in blue light region and/or the purple light district of electromagnetic spectrum.

In certain embodiments, the peak wavelength (the first peak wavelength) of the first light be about 430 to about 500nm, about 440 to about 500nm, about 450 to about 500nm, about 430 to about 475nm, approximately 435nm to about 470nm, approximately 440nm to about 460nm, approximately 440nm, approximately 450nm, approximately 460nm or about 470nm.In certain embodiments, the peak wavelength (the second peak wavelength) of the second light is about 400nm to about 500nm, approximately 400nm to about 475nm, approximately 400nm to about 450nm, approximately 400nm to about 430nm, or about 410nm to about 420nm, or about 415nm.In one embodiment, the second peak wavelength is about 410nm to about 430nm, and the peak wavelength of the first light is about 440nm to about 470nm.

Or, second peak wavelength can be about 480 to about 760nm, approximately 480nm to about 700nm, approximately 480nm to about 650nm, approximately 480nm to about 620nm, about 500 to about 700nm, approximately 520nm to about 700nm, approximately 500nm to about 660nm, approximately 540nm to about 640nm, approximately 540nm to about 580nm, approximately 500nm to about 570nm, approximately 570nm to about 590nm, approximately 590nm to about 610nm, approximately 610nm to about 760nm, or within the infra-red range of electromagnetic spectrum.

In certain embodiments, the second peak wavelength is longer than the first peak wavelength.Distance between first peak wavelength and the second peak wavelength may cause due to Stokes shift.

In the first light and the second light, the bandwidth (full width at half maximum) of at least one light is equal to or less than about 20nm, or about 19nm ± 5nm (14nm to 24nm).In certain embodiments, the bandwidth of the second light is about 15nm extremely about 100nm, approximately 25nm to about 80nm, approximately 30nm to about 70nm, or about 20nm to about 50nm.

In certain embodiments, the band of the first light of bandwidth ratio of the second light is wide.The bandwidth of the first light can be about 15nm to about 25nm, and the bandwidth of the second light can be about 20nm to about 100nm.

In certain embodiments, the average power density of that the described device that process distance (such as 5cm or 10cm) place measures is launched and/or that treatment sites receives light is less than about 200mW/cm ², be less than about 4 to about 75mW/cm ², be less than about 15 to about 75mW/cm ², be less than about 10 to about 200mW/cm ², be less than about 10 to about 150mW/cm ², be less than about 20 to about 130mW/cm ², be less than about 55 to about 130mW/cm ², be less than about 90 to about 140mW/cm ², be less than about 100 to about 140mW/cm ², or be less than about 110 to about 135mW/cm ².In certain embodiments, the average power density of light that described device is launched is about 4 to about 75mW/cm ², about 10 to about 75mW/cm ², about 10 to about 85mW/cm ², about 15 to about 75mW/cm ², about 30 to about 70mW/cm ², about 40mW/cm ²to about 70mW/cm ², about 55 to about 85mW/cm ²or about 55 to about 65mW/cm ².

In certain embodiments, the average power density of the second light is lower than the average power density of the first light.Such as, the average power density of the second light is about 0.1% of the first light to about 90%, about 0.1% to about 80%, about 0.1% to about 70%, about 0.1% to about 60%, about 0.1% to about 50%, about 0.1% to about 40%, about 0.1% to about 30%, about 0.1% to about 20%, about 0.1% to about 10%, about 0.1% to about 9%, about 0.1% to about 8%, about 0.1% to about 7%, about 0.1% to about 6%, about 0.1% to about 5%, about 0.1% to about 4%, about 0.1% to about 3%, about 0.1% to about 2%, about 0.1% to about 1%.

In certain embodiments, the average power density of the second light is less than about 75mW/cm ², be less than about 50mW/cm ², be less than about 10mW/cm ², be less than about 5mW/cm ², be less than about 2.5mW/cm ², or be less than about 2mW/cm ².The power density at peak wavelength place is about 0.02mW/cm ²/ nm to about 75mW/cm ²/ nm, about 0.02 is to about 50mW/cm ²/ nm, about 0.02 is to about 10mW/cm ²/ nm, about 0.02 is to about 5mW/cm ²/ nm or about 0.02 is to about 10mW/cm ²/ nm, about 0.005 is to about 10mW/cm ²/ nm, about 0.01 is to about 0.1mW/cm ²/ nm, about 0.01 is to about 2mW/cm ²/ nm, about 0.01 is to about 3mW/cm ²/ nm or about 0.5 is to about 5mW/cm ²/ nm.

In certain embodiments, arrange described device, thus regulate transmitting illuminant during device light emission, such as, allow transmitting power density decline in time.Power density can decline by any speed, such as, often to irradiate a minute about 0.002mW/cm ²to often irradiating a minute about 0.1mW/cm ², about 0.005mW/cm per minute ², about 0.006mW/cm per minute ²or about 0.012mW/cm per minute ²velocity of variation decline.In certain embodiments, wide fluorescence or the phosphorescence causing or substantially simulate any fluorescence or phosphorescent agent transmitting of secondary light source transmitting.In one embodiment, the light launched when the light launched of secondary light source and eosin W or W S or eosin W or W S and fluorescein photoactivation is similar.Fluorescence or phosphorescence are in visible light part or the infrared portions of electromagnetic spectrum, such as, and green glow, gold-tinted and orange light.

In certain embodiments, in single treatment, the mean energy density that device is launched and/or treatment sites receives is greater than about 4J/cm ², be greater than about 10J/cm ², be greater than about 15J/cm ², be greater than about 30J/cm ², be greater than about 50J/cm ², be no more than about 60J/cm ².In certain embodiments, arrangement apparatus, the energy density of the light that single treatment is launched is about 4J/cm ²to about 60J/cm ², about 10J/cm ²to about 60J/cm ², about 10J/cm ²to about 50J/cm ², about 10J/cm ²to about 40J/cm ², about 10J/cm ²to about 30J/cm ², about 20J/cm ²to about 40J/cm ², about 15J/cm ²to about 25J/cm ², or about 10J/cm ²to about 20J/cm ².

Processing time is about 30 seconds to about 25 minutes, about 30 seconds to about 20 minutes, about 30 seconds to 19 minutes, about 30 seconds to 18 minutes, about 30 seconds to 19 minutes, about 30 seconds to 17 minutes, about 30 seconds to 16 minutes, about 30 seconds to 15 minutes, about 30 seconds to 14 minutes, about 30 seconds to 13 minutes, about 30 seconds to 12 minutes, about 30 seconds to 11 minutes, about 30 seconds to 10 minutes, about 30 seconds to 9 minutes, about 30 seconds to 8 minutes, about 30 seconds to 7 minutes, about 30 seconds to 6 minutes, about 30 seconds to 5 minutes, about 30 seconds to 4 minutes, normally 5 to 15 minutes.Processing time can be no more than about 90 minutes, about 80 minutes, about 70 minutes, about 60 minutes, about 50 minutes, about 40 minutes or about 30 minutes.It should be understood that and can regulate the processing time, with preservation dose by regulating the transfer rate being transferred to the energy density for the treatment of sites.Such as, accumulated dose 20J/cm be realized ²with 15 minutes processing times, the transfer rate of energy density can be 1.3J/cm per minute ².In certain embodiments, largest light intensity can be processing time per minute about 12J/cm ².Therapeutic effect can be realized by adjusting energy density rate.

In certain embodiments, secondary light source is launched or the energy density that is transferred to treatment sites is launched lower than the first light source or is transferred to the energy density for the treatment of sites.Such as, secondary light source launch or the energy density that is transferred to treatment sites be the first light source launch or be transferred to treatment sites energy density about 0.1% to about 90%, about 0.1% to about 80%, about 0.1% to about 70%, about 0.1% to about 60%, about 0.1% to about 50%, about 0.1% to about 40%, about 0.1% to about 30%, about 0.1% to about 20%, about 0.1% to about 10%, about 0.1% to about 9%, about 0.1% to about 8%, about 0.1% to about 7%, about 0.1% to about 6%, about 0.1% to about 5%, about 0.1% to about 4%, about 0.1% to about 3%, about 0.1% to about 2%, about 0.1% to about 1%.

In certain embodiments, the peak wavelength of the first light is about 440nm to about 470nm, and bandwidth is about 18-24, and the peak wavelength of the second light is about 410nm to about 430nm, and bandwidth is about 13-18nm.In these embodiments, the average power density of light that described device is launched is about 55 to about 130mW/cm ², or about 10 to about 75mW/cm ².In certain embodiments, energy density rate is about 5-10J/cm per minute ².

In certain embodiments, the band of the second light is wider than the first light, and average power density is lower than the first light.The energy density of the second light may lower than the first light.The maximum power density of the second light at the second peak wavelength place is lower than the maximum power density of the first light at the first peak wavelength place.

In certain embodiments, described device comprises the 3rd light source further, and wherein the 3rd light source can emission peak wavelength be about 500nm to about 750nm, about 630 to about 750nm, or the third light of about 750nm to about 1mm.

On the other hand, the invention provides a kind of phototherapy device, comprising: the first light source launching the first light, the peak wavelength of the first light is about 400 to 750nm, and average power density is about 4 to about 75mW/cm ², or about 55mW/cm ²to about 150mW/cm ².

On the other hand, the invention provides a kind of phototherapy device, comprising: the first light source launching the first light, the peak wavelength of the first light is about 400 to about 750nm, and bandwidth is about 19nm ± approximately 5nm.

On the other hand, the invention provides a kind of phototherapy device, comprise the first light source launching the first light, the peak wavelength of the first light is about 400 to about 750nm, bandwidth is about 15nm extremely about 100nm, approximately 25nm to about 80nm, approximately 30nm to about 70nm, or about 20nm to about 50nm.

On the other hand, the invention provides a kind of phototherapy device, comprising: the first light source launching the first light, the peak wavelength of the first light is about 400 to about 750nm, and single treatment energy density is about 4 to about 60J/cm ², about 10 to about 60J/cm ², about 10 to about 50J/cm ², about 10 to about 40J/cm ², about 10 to about 30J/cm ², about 20 to about 40J/cm ², about 15J/cm ²to 25J/cm ², or about 10 to about 20J/cm ².

On the other hand, the invention provides a kind of phototherapy device being equipped with at least one light source, the band of the light that described light source is launched is wider than about 15nm, and peak wavelength is about 400nm to about 700nm, and the power density of wherein said light declines during light emission.Power density can decline by any speed, such as, often to irradiate a minute about 0.002mW/cm ²to often irradiating a minute about 0.1mW/cm ², about 0.005mW/cm per minute ², about 0.006mW/cm per minute ²or about 0.012mW/cm per minute ²velocity of variation decline.Substantially fluorescence and the decay in time of power efficiency of fluorescence density of chromophore transmitting can be simulated like this.

Above any in some embodiments in, the average power density of the first light is about 4 to about 75mW/cm ², or about 55mW/cm ²to about 150mW/cm ².

In any one some embodiments above, the energy density of the first light single treatment is about 4 to about 60J/cm ², about 10 to about 60J/cm ², about 10 to about 50J/cm ², about 10 to about 40J/cm ², about 10 to about 30J/cm ², about 20 to about 40J/cm ², about 15J/cm ²to 25J/cm ², or about 10 to about 20J/cm ².

In any one some embodiments above, the bandwidth of the first light is about 400 to about 750nm, approximately 15nm to about 100nm, approximately 25nm to about 80nm, approximately 30nm to about 70nm, or about 20nm to about 50nm.The bandwidth of the first light is about 19nm ± approximately 5nm.

In some embodiments in above, the invention provides secondary light source that have peak wavelength, that can launch the second light, wherein the second light has at least one feature in following characteristics:

A) band is wider than the bandwidth of the first light,

B) average power density is lower than the average power density of the first light,

C) power at peak wavelength place is lower than the power at the first peak wavelength place,

D) peak wavelength is longer than the peak wavelength of the first light, or

E) during process, energy density is lower than the first light.

The average power density of the second light is about 0.1% of the first light to about 90%, about 0.1% to about 80%, about 0.1% to about 70%, about 0.1% to about 60%, about 0.1% to about 50%, about 0.1% to about 40%, about 0.1% to about 30%, about 0.1% to about 20%, about 0.1% to about 10%, about 0.1% to about 9%, about 0.1% to about 8%, about 0.1% to about 7%, about 0.1% to about 6%, about 0.1% to about 5%, about 0.1% to about 4%, about 0.1% to about 3%, about 0.1% to about 2%, about 0.1% to about 1%.

Secondary light source launch or the energy density that is transferred to treatment sites be the first light source launch or be transferred to treatment sites energy density about 0.1% to about 90%, about 0.1% to about 80%, about 0.1% to about 70%, about 0.1% to about 60%, about 0.1% to about 50%, about 0.1% to about 40%, about 0.1% to about 30%, about 0.1% to about 20%, about 0.1% to about 10%, about 0.1% to about 9%, about 0.1% to about 8%, about 0.1% to about 7%, about 0.1% to about 6%, about 0.1% to about 5%, about 0.1% to about 4%, about 0.1% to about 3%, about 0.1% to about 2%, about 0.1% to about 1%.

The bandwidth of the second light can be greater than 20nm, approximately 20-100nm, approximately 20-80nm, approximately 20-60nm, approximately 20-40nm.

The second light is about 0.1% of the first light to about 90% at the power at peak wavelength place, about 0.1% to about 80%, about 0.1% to about 70%, about 0.1% to about 60%, about 0.1% to about 50%, about 0.1% to about 40%, about 0.1% to about 30%, about 0.1% to about 20%, about 0.1% to about 10%, about 0.1% to about 9%, about 0.1% to about 8%, about 0.1% to about 7%, about 0.1% to about 6%, about 0.1% to about 5%, about 0.1% to about 4%, about 0.1% to about 3%, about 0.1% to about 2%, about 0.1% to about 1%.

Any embodiment that the present invention hereafter lists can be bonded to each other, or is combined with either side mentioned above or of the present invention or embodiment.

In certain embodiments, described device comprises the controller with the first light source and secondary light source electronic communication further, for the second emission spectra of the first emission spectra and/or the second light that change the first light, wherein the first emission spectra and the second emission spectra comprise the one or more emission spectra parameters being selected from bandwidth, peak wavelength, power density, launch time and energy density.Controller can control separately one or more emission spectra parameters of the first light and the second light.In certain embodiments, controller regulates one or more emission spectra parameters of the first light and the second light according to the processing time.In certain embodiments, controller regulates one or more emission spectra parameters of the first light and the second light according to the processing time, such as, the transmitting power density of reduction by first light source is changed in time during light emission, and change in time improves the transmitting power density of secondary light source during optional light emission, to simulate fluorescence or the phosphorescence of activated chromophore transmitting.Controller can comprise the various tupes wherein processing parameter and preset, and process parameter comprises radiative density, wavelength, utilizing emitted light density and wavelength over time, and process distance.Such as, tupe comprises the different process parameter for the treatment of mild acne, severe acne, deep wrinkle, shallow wrinkle and different brackets ulcer.In certain embodiments, controller can control the pulse of any light source.

In certain embodiments, the first light source and secondary light source (if yes) can launch incoherent light.Such as, the first light source and secondary light source (if yes) can comprise light emitting diode (LED).Light emitting diode can be arranged as an array.Array can be installed in above at least one block of plate.The LED of the first light source can be considered one group of LED, and the LED of secondary light source (if yes) can be considered another group LED, often organizes LED and comprises at least one LED.Often organize LED to arrange by dispersing mode or juxtaposition.Such as, in an array, secondary light source can dispersed placement in the first light source.First light source and secondary light source (if yes), and/or the spacing distance of each LED can be about 4 to 7mm, about 4 to 10mm, about 10 to 15mm, or about 15 to 20mm.The distance that they are separated by can be substantially equal.

LED array can be arranged at least one block of plate.Described device can comprise the plate that polylith can connect.These plates can have smooth or bending light-emitting area.It can be maybe fixed configurations that these attachable plates can move relative to each other.Described device can comprise the plate controller carrying out electronic communication with these plates, the independent light emission controlling these plates.

Described device can comprise cooling system, and cooling system can comprise the radiator, the fan of cooling LED array, the air vent carried on the crust of the device of light source that are connected with LED array, and heat is removed by convection current, heat screen or other means any.

Described device also can comprise the wave filter filtered the first light and/or the second light (if yes).Described device comprises collimator further, for calibrating the first light and/or the second light (if yes).Described device comprises spacing probe that stretch out from the described device surface of emission, that be used to indicate process distance further.

In another embodiment, light source or LED array can be placed on above flexible substrate (such as fabric), use together with dressing or veil, or form a part for dressing or veil.

The light emitting surface of described device can comprise one or more waveguide pipe that can be connected with one or more light source, such as optical fiber or fibre bundle.Optical fiber can adopt any applicable optical transport and have the material of suitable tensile property, and such as polymethyl methacrylate (PMMA) manufactures.Optical fiber can be contained in cover.Therefore, optical fiber light can be transferred to experimenter from device internal cavity in or experimenter be difficult to the therapentic part that arrives with it.

In some embodiments in above-mentioned, light source can launch purple light scope (400-450nm), blue light range (450-490nm), electromagnetic spectrum green range (about 490 to about 560nm), yellow range (560-590nm), orange optical range (590-635), or the light in electromagnetic spectrum red range (about 635 to about 750nm).Emission wavelength mentioned above and power density, energy density and/or bandwidth can photoactivation bio-photon compositionss, and/or itself has therapeutic effect.

In certain embodiments, the peak wavelength of the light that the first light source is launched is about 440-470nm, and bandwidth is the overall power density at about 20nm ± 2nm, 5cm place is about 100-150mW/cm ², 60-135mW/cm ², about 135mW/cm ².In another embodiment, the peak wavelength of light that the first light source is launched is that the overall power density at about 440-480nm, 5cm or 10cm place is less than about 75mW/cm ², about 25 to about 70mW/cm ², about 30 to about 65mW/cm ², about 55 to about 65mW/cm ².In another embodiment, described device comprises secondary light source, and secondary light source can emission peak wavelength be about 410nm to about 420nm, and bandwidth is about 13-15nm, and overall power density is 0.01 to 5mW/cm ²light.

It is found that, wavelength is in purple light/blue light range, the at least one light source of the about 440-470nm of peak value can excite yellow/orange/orchil, such as eosin W or W S, fluorescein, rose-red, phloxine B and erythrosine, the present inventor finds, these dyestuffs, together with purple light/blue light, have useful effect to treatment sites, such as, regulate blood flow and regulate collagen protein.

In certain embodiments, the light that light source is launched, except as can except the activation light of chromophore in photoactivatable composition, when being administered to tissue, itself also has treatment benefit, such as antibacterial characteristics, the blood flow regulating treatment sites, adjustment collagen protein, or any other beauty treatment or therapeutic treatment effect.Therefore, be favourable by apparatus of the present invention with using together with photoactivatable composition; Meanwhile, this can make activation light pass can photoactivatable composition, is irradiated to the tissue that application of described compositions.Can the compositions of photoactivation be transparent or translucent substantially, or can be guide-lighting.

First light source, secondary light source and/or the 3rd light source can launch the light of heterogeneity/complementary character from single light source or multiple light source simultaneously, at different time and/or different time cycles.Like this, described device can be used for each stage of disease therapy, such as, first treats wound infection, then for reducing inflammation, regulating collagen protein, farthest to reduce the cicatrix produced in wound healing process.Another example is Acne treatment, first kills the antibacterial (such as propionibacterium acnes (p.acnes)) patient skin causing disease, then, is regulated, treat acne lesion and cicatrix by vascularization and collagen protein.

Device in above-mentioned any embodiment can be lamp holder or lamp itself.When described device is lamp holder, it can exchange with other lamp holder, is arranged on identical holder structure.The light that the configurable emission parameter of light source (such as wavelength, pulse duration, total emission energy) on different lamp holder is different, or different lamp holders has the different size and dimension being applicable to the different body part for the treatment of.

On the other hand, the invention provides a kind of lamp, comprise lamp holder, lamp holder has multiple layout light emitting diode in an array (LED), and array comprises at least two group LED, wherein often organizes LED and comprises at least one LED; Lamp controller, lamp controller is electrically connected with lamp holder, has the circuit controlling and operate LED; Wherein first group of LED can produce the incoherent light of the about 430nm of peak wavelength to about 500nm; Wherein second group of LED can produce the incoherent light of the about 400nm of peak wavelength to about 430nm; The power density of light that wherein lamp holder produces is about 10 to about 75mW/cm ², or about 55mW/cm ²to about 150mW/cm ².

On the other hand, the invention provides a kind of lamp, comprise lamp holder, lamp holder has multiple layout light emitting diode in an array (LED), array comprises at least the first group LED and second group LED, wherein first group of LED and second group LED often group comprise at least one LED; Lamp controller, lamp controller is electrically connected with lamp holder, has the circuit controlling and operate LED; Wherein first group of LED can produce the incoherent light of the about 430nm of peak wavelength to about 500nm; Wherein second group of LED can produce the incoherent light of the about 400nm of peak wavelength to about 430nm; The power density of light that wherein lamp holder produces or treatment surface receives is about 4 to about 75mW/cm ², or about 55mW/cm ²to about 150mW/cm ².

On the other hand, the invention provides a kind of lamp, comprise lamp holder, lamp holder has multiple layout light emitting diode in an array (LED), array comprises at least the first group LED and second group LED, wherein first group of LED and second group LED often group comprise at least one LED; Lamp controller, lamp controller is electrically connected with lamp holder, has the circuit controlling and operate LED; Wherein first group of LED can produce the incoherent light of the about 400nm of peak wavelength to about 500nm; Wherein second group of LED can produce the incoherent light that peak wavelength is about 500nm to about 760nm; The power density of light that wherein lamp holder produces is about 4 to about 75mW/cm ², or about 55 to about 150mW/cm ².

First group of LED can the roomy about 19nm ± 5nm of generating strap, or the light of about 13 to about 26nm.Second group of LED can produce the light that bandwidth is about 13nm to about 20nm.Described lamp may further include the 3rd group of LED, and wherein the 3rd group of LED can produce the about 500nm to 750nm of peak wavelength, or the incoherent light of about 630nm to about 720nm.

Described lamp may further include controller, and controller is for changing one or more parameters of first and/or the second light, and one or more parameter is selected from power density, bandwidth, wavelength, integral energy and launch time.

In certain embodiments, first group of LED and second group LED is spaced about 4 to 7mm, about 4 to 10mm, about 10 to 15mm in an array, or about 15 to 20mm.First group of LED and second group LED can dispersed placement each other.First group of LED and second group LED in an array can equidistant placement substantially.Lamp holder can comprise the plate that polylith puts LED, and described plate can move relative to each other.Lamp controller preferably can control separately the LED of every block plate.

Described lamp may further include one or more wave filter of filtration first and/or the second light.Described lamp also may further include the collimator of calibration first and/or the second light.Lamp also can comprise spacing probe that stretch out from the lamp surface of emission, that be used to indicate process distance further.Lamp holder comprises at least one air vent for cooling.Also other cooling system can be used.

During single treatment, lamp can be launched or transmit about 4 to about 60J/cm to treatment surface ², about 10 to about 60J/cm ², about 10 to about 50J/cm ², about 10 to about 40J/cm ², about 10 to about 30J/cm ², about 20 to about 40J/cm ², about 15J/cm ²to 25J/cm ², or about 10 to about 20J/cm ²energy density.Lamp can launch or transmit about 10 to about 75mW/cm ², about 15 to about 75mW/cm ², about 10 to about 150mW/cm ², about 20 to about 130mW/cm ², about 55 to about 130mW/cm ², about 90 to about 140mW/cm ², about 100 to about 140mW/cm ², about 110 to about 135mW/cm ², about 10 to about 85mW/cm ², about 30 to about 70mW/cm ², about 40 to about 70mW/cm ², about 55 to about 65mW/cm ², or about 55 to about 85mW/cm ²power density.

In certain embodiments, device or lamp are of portable form.It can be equipped with wheel or handle.Described device or lamp can comprise and being installed on furniture (shelf on such as bed or wall) by lamp.Described device or lamp can also comprise the ground support of bracing or strutting arrangement or lamp, such as supporting leg, leg, wheel or base.Support can comprise ponderable base or long foot, can in furniture slip underneath, and anti-locking apparatus or lamp are toppled over.Shelf or support can fold, and conveniently store and carry.The portable version of device or lamp can adopt battery-operated or can again charge, and can be equipped with cable or not be equipped with cable.Power supply, such as battery, can be included in the cradle that can be connected with lamp, or in lamp body.

In an embodiment of portable hand-held lamp, the diameter of the circular surface of emission of lamp holder is such as about 5cm to about 10cm.In this case, in order to process the diameter treatment sites larger than beam diameter, adopt the LED that the angle of flare is wider, in order to the region larger than emitting surface diameter to diameter provides light, the distance of portable lamp and treatment sites should keep enough far away.In one embodiment, the density of light is about 75 to about 120mW/cm ².Wavelength is about 420-490nm, and peak wavelength is about 460-470nm.

On the other hand, the invention provides a kind of device with at least one light source, launch and the fluorescence that activated chromophore is launched or the substantially corresponding light of phosphorescence.In one embodiment, the band of the light of light source transmitting is wider than about 15nm, is greater than about 20nm, is greater than about 25nm, is greater than about 30nm.Such as, bandwidth can be about 15nm extremely about 100nm, approximately 25nm to about 80nm, approximately 30nm to about 70nm, or about 20nm to about 50nm.In certain embodiments, arrange described device, thus regulate transmitting illuminant during device light emission, such as, allow transmitting power density decline in time.In certain embodiments, at least one light source emission peak wavelength is the light of about 400nm to about 750nm, approximately 480nm to about 700nm, approximately 500nm to about 660nm, approximately 540nm to about 640nm.In one embodiment, light source produces overall power density is 0.005 to about 10mW/cm ², about 0.01 to 0.1mW/cm ², about 0.01 to about 2mW/cm ², about 0.01 to about 3mW/cm ², about 0.5 to about 5mW/cm ²light.

Power density can decline by any speed, such as, often to irradiate a minute about 0.002mW/cm ²to often irradiating a minute about 0.1mW/cm ², about 0.005mW/cm per minute ², about 0.006mW/cm per minute ²or about 0.012mW/cm per minute ²velocity of variation decline.

On the other hand, the invention provides the purposes that said apparatus or lamp use together with light activatable compositions.In certain embodiments, light activating agent can be comprised by photoactivatable composition.Light activating agent can be fluorescent agent, such as dyestuff.Light activating agent can absorb or launch at least one light in electromagnetic spectrum green glow, gold-tinted, orange light or red light portion.In one embodiment, light activating agent is a kind of optical medium being applied to tissue or being placed in adjacent tissue, common peak excitation wavelength (in water or in ethanol) is about 400 to about 700nm, approximately 420-565nm, approximately 420-540nm, approximately 470-535nm, or about 450nm to about 700nm.In certain embodiments, can photoactivatable composition comprise in eosin W or W S, fluorescein, rose-red, erythrosine, phloxine B, chlorophyll a, chlorophyll b, CHLOROPHYLLINE one or more.

On the other hand, the invention provides the beautifying use of said apparatus or lamp or tissue beauty therapeutic purposes (such as skin renewal, skin condition, skin care, reduce or eliminate cicatrix, eliminate tatoo, make the colour of skin evenly, reduce pore, increase tissue intensity, reduce or eliminate cicatrix), medical application (such as wound healing), treatment inflammation, treatment antibacterial, virus or fungal infection, the purposes for the treatment of dermatosis (such as acne, acne erythematosa, psoriasis, eczema, dermatitis) and/or diagnostic uses.Described device and/or lamp can use anywhere, are included in the uses such as family, hospital, clinic, scene.

In some embodiments of such use, described device can be movable fixture, such as hand-held computing device, smart phone, or band display screen or flash lamp are as the mobile phone of emitting surface, such as samsung in these embodiments, as of the present invention, can use and launch that have can to can photoactivatable composition " application " of emission spectra that activate, within the scope that they are included in the present patent application.Equally, the display screen of desktop computer or TV is applicable to launching that have can to can the light of emission spectra that activates of photoactivatable composition.Therefore, therapentic part only need be placed near the light emission surface of mobile device or display screen by user, from light and can be benefited the therapeutic effect of photoactivatable composition.TV, computer display or mobile device may have a light source, this light source by the irradiation of about 15 minutes, 30 minutes, 45 minutes, 1 hour, 2 hours, 3 hours, 4 hours, 5 hours, 6 hours, 7 hours or 8 hours to carrying out photoactivation by photoactivatable composition, and/or this light source can launch emission spectra with can the absorption spectrum of light activating agent is overlapping in photoactivatable composition light.

Device of the present invention and lamp can be placed in the objects such as mobile device, TV, computer, bathtub, shower, bed, sun tan bed, clothing apparel, blanket, overcover.

On the other hand, the invention provides a kind of for organizing the beauty method that processes or medical procedures, described method comprises: with have defined above (device in describing the present invention and lamp launch light time involved) the photo-irradiation treatment position of emission spectra.Such as, two kinds of light that treatment sites can be different with the emission spectra in electromagnetic spectrum blue light and/or purple light district irradiate.

On the other hand, the invention provides and a kind ofly organize beauty treatment or the method for Medical Treatment, described method comprises: adopt have can to can the first illumination of the first peak wavelength of activating of photoactivatable composition penetrate be applied in above organized processing position or neighbouring can photoactivatable composition or irradiate the treatment sites of tissue; Adopt the second illumination penetrate identical or different on organized processing position can photoactivatable composition or irradiate the treatment sites of described tissue, the second light has the second peak wavelength, second peak wavelength is different from the first peak wavelength, and wherein the first peak wavelength and the second peak wavelength are all in blue light and/or the purple light district of electromagnetic spectrum.

On the other hand, the invention provides a kind of method organizing beauty treatment or Medical Treatment, described method comprises: adopt the first illumination with the first peak wavelength to penetrate described tissue; Adopt the second illumination with the second peak wavelength to penetrate described tissue, the second peak wavelength is different from the first peak wavelength, and wherein the first peak wavelength is in blue light and/or the purple light district of electromagnetic spectrum.

On the other hand, the invention provides a kind of method organizing beauty treatment or Medical Treatment, described method comprises: adopt the incoherent light from the first light source, the about 430nm of peak wavelength to about 500nm to irradiate tissue; The incoherent light from secondary light source, the about 400nm of peak wavelength to about 430nm is adopted to irradiate tissue; Wherein said tissue overall power density about 4 is to about 75mW/cm ², or about 55mW/cm ²to about 150mW/cm ²light irradiate.

In certain embodiments, use on organized processing position or near treatment sites before being included in further and irradiating tissue can the compositions of photoactivation for described method.

First peak wavelength is about 430 to about 500nm, about 440 to about 500nm, about 450 to about 500nm, about 430 to about 475nm, approximately 440nm to about 460nm, approximately 445nm to about 455nm, approximately 435nm to about 470nm, approximately 440nm, approximately 450nm, approximately 460nm or about 470nm.The peak wavelength of the second light is about 400nm to about 500nm, approximately 400nm to about 475nm, approximately 400nm to about 450nm, approximately 400nm to about 430nm, or approximately 410nm to about 420nm or approximately 415nm.Such as, the first peak wavelength may be about 410nm to about 430nm, and the second peak wavelength may be about 440nm to about 470nm.

In certain embodiments, a kind of bandwidth of light is had at least to be equal to or less than about 20nm or about 19nm ± 5nm in the first light and the second light.The bandwidth of the second light is about 15nm extremely about 100nm, approximately 25nm to about 80nm, approximately 30nm to about 70nm, or about 20nm to about 50nm.The bandwidth of the second light can wide than the first light.Such as, the bandwidth of the first light can be about 15nm to about 25nm, and the bandwidth of the second light is about 20nm to about 100nm.

The first light and the power density that the second is photoemissive and/or treatment sites receives can be about 4 to about 75mW/cm ², about 15 to about 75mW/cm ², about 10 to about 200mW/cm ², about 10 to about 150mW/cm ², about 20 to about 130mW/cm ², about 55 to about 130mW/cm ², about 90 to about 140mW/cm ², about 100 to about 140mW/cm ², or about 110 to about 135mW/cm ².

The first light and the power density that the second is photoemissive and/or treatment sites receives can be about 4 to about 75mW/cm ², about 15 to about 75mW/cm ², about 10 to about 85mW/cm ², about 10 to about 75mW/cm ², about 30 to about 70mW/cm ², about 40 ²to about 70mW/cm ², about 55 to about 65mW/cm ², or about 55 to about 85mW/cm ².

In certain embodiments, the average power density of the second light is lower than the average power density of the first light.The average power density of the second light is about 0.1% of the first light to about 90%, about 0.1% to about 80%, about 0.1% to about 70%, about 0.1% to about 60%, about 0.1% to about 50%, about 0.1% to about 40%, about 0.1% to about 30%, about 0.1% to about 20%, about 0.1% to about 10%, about 0.1% to about 9%, about 0.1% to about 8%, about 0.1% to about 7%, about 0.1% to about 6%, about 0.1% to about 5%, about 0.1% to about 4%, about 0.1% to about 3%, about 0.1% to about 2%, about 0.1% to about 1%.

During single treatment, the energy density of the light that treatment sites receives can be greater than about 4J/cm ², be greater than about 10J/cm ², be greater than about 15J/cm ², be greater than about 30J/cm ², be greater than about 50J/cm ², be no more than 60J/cm ².During single treatment, the energy density of the light that treatment sites receives is about 4J/cm ²to about 60J/cm ², about 10J/cm ²to about 60J/cm ², about 10J/cm ²to about 50J/cm ², about 10J/cm ²to about 40J/cm ², about 10J/cm ²to about 30J/cm ², about 20J/cm ²to about 40J/cm ², about 15J/cm ²to about 25J/cm ², or about 10J/cm ²to about 20J/cm ².

In certain embodiments, single treatment comprises irradiates tissue or can be no more than about 90 minutes by photoactivatable composition, 80 minutes, 70 minutes, 60 minutes, 50 minutes, 40 minutes, 30 minutes, about 1 to about 30 minutes, about 1 to about 25 minutes, about 1 to about 20 minutes, about 1 to about 19 minutes, about 1 to about 18 minutes, about 1 to about 17 minutes, about 1 to about 16 minutes, about 1 to about 15 minutes, about 1 to about 14 minutes, about 1 to about 13 minutes, about 1 to about 12 minutes, about 1 to about 11 minutes, about 1 to about 10 minutes, about 1 to about 9 minutes, about 1 to about 8 minutes, about 1 to about 7 minutes, about 1 to about 6 minutes, about 1 to about 5 minutes, about 1 to about 4 minutes, about 1 to about 3 minutes.Light can use by mode that is continuous or pulsed exposure.

In certain embodiments, secondary light source is transferred to the energy density for the treatment of sites lower than the first light source transmitting or the energy density being transferred to treatment sites.The energy density that each process secondary light source is transferred to treatment sites is about 0.1% to about 90% of the first light source transmitting or the energy density being transferred to treatment sites, about 0.1% to about 80%, about 0.1% to about 70%, about 0.1% to about 60%, about 0.1% to about 50%, about 0.1% to about 40%, about 0.1% to about 30%, about 0.1% to about 20%, about 0.1% to about 10%, about 0.1% to about 9%, about 0.1% to about 8%, about 0.1% to about 7%, about 0.1% to about 6%, about 0.1% to about 5%, about 0.1% to about 4%, about 0.1% to about 3%, about 0.1% to about 2%, about 0.1% to about 1%.

In certain embodiments, the band of the second light is wider than the first light, and average power density is lower than the first light.The energy density of the second light may lower than the first light.

In certain embodiments, the peak power at the second peak wavelength place is lower than the peak power at the first peak wavelength place.

Described method changes the emission spectra of the first light and/or the second light during can being included in single treatment further, wherein the emission spectra of the first light and the second light comprises one or more emission spectra parameters being selected from bandwidth, peak wavelength, power density, launch time and energy density.

On the other hand, the invention provides a kind of method organizing beauty treatment or Medical Treatment, comprise and adopt peak wavelength about 400 to about 750nm, power density about 4 is to about 75mW/cm ², or about 55mW/cm ²to about 150mW/cm ²the first illumination penetrate tissue.

On the other hand, provide a kind of method organizing beauty treatment or Medical Treatment, comprise and adopt peak wavelength about 400 to about 750nm, tissue is penetrated in the first illumination of the about 19nm of bandwidth ± about 5nm.

On the other hand, a kind of method organizing beauty treatment or Medical Treatment is provided, comprise and adopt peak wavelength about 400 to about 750nm, the about 15nm of bandwidth is about 100nm, approximately 25nm to about 80nm, approximately 30nm to about 70nm extremely, or tissue is penetrated in the first illumination of about 20nm to about 50nm.

On the other hand, a kind of method organizing beauty treatment or Medical Treatment is provided, comprise and adopt peak wavelength about 400 to the first illumination of about 750nm to penetrate tissue, during irradiated tissue, regulate at least one parameter in the peak wavelength of the first light, bandwidth, power density or energy density.

On the other hand, provide a kind of method organizing beauty treatment or Medical Treatment, comprise and adopt peak wavelength about 400 to about 750nm, during single treatment, energy density about 4 is to about 60J/cm ², about 10 to about 60J/cm ², about 10 to about 50J/cm ², about 10 to about 40J/cm ², about 10 to about 30J/cm ², about 20 to about 40J/cm ², about 15J/cm ²to 25J/cm ², about 10 to about 20J/cm ²the first illumination penetrate tissue.

Described method comprises and adopts peak wavelength about 400 to the first illumination of about 750nm to penetrate tissue, regulates at least one parameter in the peak wavelength of the first light, bandwidth, power density or energy density during irradiated tissue.In one embodiment, described method is included in illumination and penetrates the maximum power density that the light that at least one light source is launched is reduced or improved to period.The light of Different Light can carry out different adjustments in the different time or simultaneously.Will be appreciated that and may only regulate the light of a light source within the time of a total irradiation time part, or all regulate in whole irradiation time.In certain embodiments, power density can raise by any speed or decline, and such as, speed often irradiates a minute about 0.002mW/cm ²to often irradiating a minute about 0.1mW/cm ², about 0.005mW/cm ²/ minute, about 0.006mW/cm ²/ minute, or about 0.012mW/cm ²/ minute.

The multiple light sources that treatment sites can adopt single source or have a different qualities simultaneously or irradiate at different time.Irradiate light and can have any character described when relating to device and lamp above.

In certain embodiments, irradiate the fluorescence within one or more optical ranges in only electromagnetic spectrum in green glow, gold-tinted, orange light, HONGGUANG and infrared portions or phosphorescence, such as, peak wavelength is within about 490nm to about 720nm scope.In certain embodiments, the wavelength irradiating light is about 400nm to about 700nm, approximately 480nm to about 700nm, approximately 500nm to about 660nm, approximately 540nm to about 640nm.In another embodiment, irradiating the power density of light is 0.005 to about 10mW/cm ², about 0.5 to about 5mW/cm ².In certain embodiments, the bandwidth of irradiating light is about 15nm extremely about 100nm, approximately 25nm to about 80nm, approximately 30nm to about 70nm, or about 20nm to about 50nm.The light source irradiating light can be light activating agent, such as the fluorescent dye of emitting fluorescence by the first light source or other light source any activation.Such as, or irradiating light can be the light that the electronic equipments of simulating fluorescence or phosphorescence spectrum produces, LED, laser instrument etc.

In certain embodiments, average power density or the overall power density of irradiating light are about 0.01mW/cm ²to about 200mW/cm ², 0.02mW/cm ²to about 150mW/cm ², 0.02mW/cm ²to about 135mW/cm ², 0.02mW/cm ²to about 75mW/cm ², 0.02mW/cm ²to about 60mW/cm ², about 0.02mW/cm ²to about 50mW/cm ², about 0.02mW/cm ²to about 30mW/cm ², about 0.02mW/cm ²to about 15mW/cm ².

Those of ordinary skill in the art are noted that the combination of the embodiment that also can adopt irradiation light mentioned above.Such as, simultaneously or different time adopt the photo-irradiation treatment position with different emission spectra.In one embodiment, the about 440-470nm of peak wavelength is adopted, the about 20nm ± 2nm of bandwidth, overall power density or the about 60-150mW/cm of average power density ²the light of the first light source; The about 540nm of peak wavelength to about 640nm, power density about 0.005 is to about 10mW/cm ², about 0.5 to about 5mW/cm ², the about 20nm of bandwidth is about 100nm, approximately 25nm to about 80nm, approximately 30nm to about 70nm extremely, or the photo-irradiation treatment position of the secondary light source of about 20nm to about 50nm.

On the other hand, a kind of system processing tissue is provided, described system comprises: the light source at least one treatment with irradiation position, at least one light source described can launch the irradiation light of the emission spectra with following one or more characteristic: (i) produces antibacterial effect in the light-struck treatment sites of transmitting, (ii) blood flow for the treatment of sites is regulated, and/or (iii) activation comprise chromophore can the compositions of photoactivation, then the latter can launch the light with therapeutic effect and maybe can discharge above biology-therapeutic activity kind to treatment sites, the inside or near, thus can and/or cell can not will be made irritated by cell internalizing by the light activating agent in photoactivatable composition.The multiple light sources that treatment sites can adopt single source or have a different qualities simultaneously or irradiate at different time.Irradiate light and can have any character described when relating to device, lamp and/or system above.

On the other hand, provide a kind of system processing tissue, described system comprises: lamp mentioned above or device, and comprise activator can photoactivatable composition.

In certain embodiments, the peak wavelength of light that at least one light source is launched be about 400nm to about 720nm, 400nm to about 550nm, approximately 450nm to about 500nm, about 440 to about 475nm, about 450, about 446, about 464nm or about 470nm.Now, light source can be at least one LED or any LED array.

In certain embodiments, irradiate the fluorescence within one or more optical ranges in only electromagnetic spectrum in green glow, gold-tinted, orange light, HONGGUANG and infrared portions or phosphorescence, such as, peak wavelength is within about 520nm to about 720nm scope.In this case, described system comprises the light activating agent as light source.In certain embodiments, the wavelength irradiating light is about 400nm to about 700nm, approximately 480nm to about 700nm, approximately 500nm to about 660nm, approximately 540nm to about 640nm.In another embodiment, irradiating the power density of light is 0.005 to about 10mW/cm ², about 0.5 to about 5mW/cm ².In certain embodiments, the bandwidth of irradiating light is about 20nm extremely about 100nm, approximately 25nm to about 80nm, approximately 30nm to about 70nm, or about 20nm to about 50nm.The light source irradiating light can be light activating agent, such as the fluorescent dye of emitting fluorescence by the first light source or other light source any activation.Or light source can be the LED array of substantially simulating fluorescence or phosphorescence spectrum.

In certain embodiments, light source can produce the about 400nm of peak wavelength to about 500nm, optional approximately 400nm to about 475nm, optional approximately 400nm to about 450nm, optional approximately 410nm to about 420nm, or the light of about 415nm to about 418nm in certain embodiments.Now, light source can be at least one LED or any LED array.

Those of ordinary skill in the art are noted that the combination of one or more light source embodiment mentioned above also may belong to the scope of system of the present invention.Such as, described system can comprise the about 450nm of peak wavelength, bandwidth about 20nm or lower, the average or about 130-150mW/cm of overall power density ²the first light source; And secondary light source, secondary light source be can emitting fluorescence or phosphorescence can photoactivatable composition.In one embodiment, the peak wavelength of fluorescence is about 540nm to about 640nm, and power density is 0.005 to about 10mW/cm ², about 0.5 to about 5mW/cm ², bandwidth is about 20nm extremely about 100nm, approximately 25nm to about 80nm, approximately 30nm to about 70nm, or about 20nm to about 50nm.

In certain embodiments, during described system comprises process, regulate the controller irradiating light, such as, regulate in the processing time and irradiate the average of light or maximum power density or regulate bandwidth or wave-length coverage.In one embodiment, described method is included in illumination and penetrates maximum power density or the average power density that the light that at least one light source is launched is reduced or improved to period.The light of Different Light can carry out different adjustments in the different time or simultaneously.Any radiative power density can decline by any speed, and such as, fall off rate often irradiates a minute about 0.002mW/cm ²to often irradiating a minute about 0.1mW/cm ², about 0.005mW/cm ²/ minute, about 0.006mW/cm ²/ minute, or about 0.012mW/cm ²/ minute.Will be appreciated that can only regulating power density in the portion of time of total irradiation time, or all regulate in whole irradiation time.

On the other hand, the invention provides a kind of beauty treatment or the medical procedures that process tissue, described method comprises the first illumination of employing and penetrates organized processing position, and the power density of the first light declines within the time at least partially of total irradiation time.Optional, described method comprises penetrates same treatment sites with the second illumination, and the power density of the second light increases, remains unchanged or decline within the time at least partially of total irradiation time.In one embodiment, the first light has one or more that many-kind of color bandwidth sum comprises in green, yellow, orange or red light.In one embodiment, the wave-length coverage of the second light is within the scope of monochromatic light, comprises blue light or purple light.In certain embodiments, the wavelength of the first light and/or the second light and/or bandwidth are also changes within the time at least partially of total irradiation time.In another embodiment, the first is only produced by least one fluorescent dye in treatment sites or near treatment sites in compositions.Described method can comprise the step of the power density regulating the first light or the second light further, this adjustment is the thickness by changing compositions, the distance between change light source and treatment sites, change the concentration of fluorescent dye in compositions, or in fluorescent dye, add that chemical seed (such as halogenide) strengthens fluorescence to realize.

On the other hand, the invention provides a kind of system processing tissue, described system comprises the first light source, and the first light source can produce the first light declined in the internal power density of time at least partially of total irradiation time.Optional, described system comprises secondary light source, and secondary light source can produce the second light raising in the internal power density of time at least partially of total irradiation time, remain unchanged or decline.In one embodiment, the first light has one or more that many-kind of color bandwidth sum comprises in green, yellow, orange or red light.In one embodiment, the wave-length coverage of the second light is within the scope of monochromatic light, comprises blue light or purple light.In certain embodiments, the wavelength of the first light and/or the second light and/or bandwidth are also changes within the time at least partially of total irradiation time.In another embodiment, the first light source comprises at least one fluorescent dye in treatment sites or near treatment sites in compositions.

The tissue that wavelength that is independent and combination, bandwidth, transmitting power density and intensity are considered to irradiate for it above has certain therapeutic effect supplemented, but, be not limited to this.Inventor finds, the light of the about 410-490nm of peak wavelength has blood flow accommodation property to treatment sites, may have the characteristic of antiinflammatory and adjustment collagen protein.The present inventor also finds, the blue light of the about 410-490nm of peak wavelength, the about 15-25nm of bandwidth can green, yellow and orange above photoactivation tissue or in adjacent tissue optical medium, makes them send fluorescence.According to Stokes shift, the peak wavelength of emitting fluorescence is longer than activation light, and wavelength is longer, and tissue infiltration is darker.The fluorescence launched can be many colors bandwidth, and this bandwidth is considered to strengthen its potential therapeutic effect.Therefore, we think, the fluorescence of transmitting also has therapeutic effect to its organizing of irradiating, and the therapeutic effect of the light that this effect and light source are launched complements each other.In the presence of oxygen, photoactivation can also produce the reactive oxygen kind with therapeutic effect.

definition

It must be noted that, just as used in this specification and the appended claims like that, unless the clear display of context, otherwise singulative " ", " one " and " being somebody's turn to do " comprise plural referents.

The word " approximately " used when providing numerical value or scope in the present invention refers to the difference of numerical value or scope and the numerical value provided or scope within 20%, preferably within 10% and more preferably within 5%.

Use in the present invention " and/or " referring to each feature or composition of specifically disclosing two kinds of regulation features or composition, both can have concurrently simultaneously or have separately.Such as, " A and/or B " refers to and discloses (i) A, (ii) B and (iii) A and B tri-kinds of situations, and often kind of situation is all independently.

" antibacterial effect " refers to and can kill or the microorganism such as anti-bacteria, fungus and protozoon.It comprises bactericidal effect and fungistatic effect.

" emission spectra " refers to the frequency spectrum of the electromagnetic radiation of definition utilizing emitted light character, usually represents with wavelength, power density and bandwidth.

" light emitting diode " refers to any light emitting diode, comprises Organic and polymer materials, solid-state and RGB light emitting diode." light source " refers to any light source of the light that can export the characteristic with expectation, such as light emitting diode; Incandescent lamp bulb; Electronic stimulation light source; The glimmering luminescent material of electricity, the glimmering material of electrostrictive polymer of such as electric glimmering line and plate, electric field induce; Gas discharge bulb, such as fluorescent lamp, cathode modulation, neon lamp and argon lamp, plasma lamp, xenon flash lamp; High-intensity discharge lamp, such as metal-halide lamp, laser diode, fibre laser, arc discharge lamp or other light source.Light source can emission spectra concentrate or the pulsed light wave in (i.e. broadband) of spectral dispersion or continuous light wave.In the present invention, a kind of light source can be also regarded as by radiative light activating agent.Light source can be understood as and also can comprise one group and have similar characteristics, one or more light-emitting devices of such as similar wavelength.

The emission spectra that the emission spectra with " peak value " refers to light is narrow bandwidth spectrum or quasi-monochromatic light spectrum.

" peak wavelength " refers to wavelength when light has peak power or intensity, usually in units of nm.

" power ", " intensity ", " power density ", " mean power " refer to the radiancy of light, and be defined as per unit surface area radiant power, usual unit is W/cm ²or mW/cm ².Radiant power is power/unit interval, and unit is watt (W).

" peak wavelength place peak power " refers to the peak power value at the peak wavelength place of light, and usual unit is mW/cm ²/ nm.

" general power at peak ", " general power " or " average power density " of light refers to the power at all wavelengths place at this peak, and usual unit is mW/cm ².

" bandwidth " refers to the spectral bandwidth of maximum intensity one half, i.e. FWHM (full width at half maximum).

" energy density " refers to unit are radiant power (being also called " power " or " intensity " or " power density ") and is multiplied by the time.The unit normally J/cm of energy density ².Energy density is also called dosage.

The compositions of photoactivation " can " refers to any medium comprising chromophore, and wherein the molecule of chromophore can radiant energy in absorbing medium, causes launching the light absorbed, such as, emitting fluorescence, or make chromophore molecule transit to excited state, then with other interaction of molecules.Use " excited state " this noun herein, it and " light activated " or " photoactivation " can exchange use.

Word " chromophore ", " photoactivated reagents " and " light activating agent " can exchange use.Chromophore refers to can light absorbing chemical molecular or compound when illumination is penetrated.

According to detailed description below, other suitable application area of device of the present invention, lamp, purposes and method will be apparent.It should be understood that detailed description and instantiation, while showing specific embodiment, its object is just for illustrating the present invention, instead of the scope of restriction the present invention or any claim.

Accompanying drawing explanation

With reference to accompanying drawing, and according to hereafter further instruction, will more fully understand above and other objects of the present invention and advantage.These embodiments described are interpreted as exemplary instead of restrictive.

Fig. 1 is the schematic diagram that certain embodiments of the invention comprise the light-emitting device of the first light source and secondary light source.

Fig. 2 is the exemplary physical layout drawings of array of source of Fig. 1 certain embodiments of the invention device.

Fig. 3 is the radiative spectrogram that Fig. 1 some embodiments of the invention device distance light source 5cm place measures.

Fig. 4 a-Fig. 4 d is the alternative exemplary physical layout drawings (Fig. 4 a-Fig. 4 d) of some embodiments of the invention array of source.

Fig. 5 is the radiative spectrogram that Fig. 1 some embodiments of the invention another kind of device for carrying out said distance light source 5cm place measures.

Fig. 6 is to perform the exemplary block diagram of the computing equipment of any function according to some embodiments of the present invention.

Fig. 7 a is one and launches spectrogram, indicates some embodiments of the invention (example 1) power density and changes, for evaluating vascularization with the optical processing time be administered on cell; Fig. 7 b is the enlarged drawing of a part for Fig. 7 a emission spectra.

Fig. 8 shows that average power density declined with launch time, and the power density decline situation at the light source different distance place apart from the fluorescence launched by the compositions of the device of some embodiments in the present invention or the photoactivation of system (example 3) institute.

Fig. 9 shows that average power density increased with launch time, and the power density at the light source different distance place apart from the fluorescence launched by the compositions of the device of some embodiments in the present invention or the photoactivation of system (example 3) institute increases situation.

Figure 10 a is one and launches spectrogram, indicates some embodiments of the invention (example 3) power density with using

Change, for evaluating vascularization to the optical processing time on cell; Figure 10 b is Figure 10 a emission spectra

The enlarged drawing of a part.

Figure 11 a indicates the impact (example 4) of light on bacterial growth of device described in some embodiments of the invention.

Figure 11 b indicates the impact (example 4) of light on bacterial growth of device described in some embodiments of the invention.

Detailed description of the invention

In order to understand device described herein, lamp, system and method, now some exemplary embodiment will be described.For the purpose of clarifying and illustrate, embodiment herein relates generally to light emitting diode (LED) light source.But those of ordinary skill in the art will be appreciated that in apparatus of the present invention, lamp, system and method also can use other light source, they can be applicable to the present invention through change and transformation.Such increase and transformation do not deviate from scope of the present invention.

According to the aspect of first broad sense of the present invention, the invention provides a kind of light-emitting device 100, described device 100 is a kind of lamps, comprises the first light source and secondary light source.Particularly, the first light source is first group of LED102 in one embodiment, and its emission spectra can induce the treatment sites of light source irradiation or region to produce therapeutic effect.Therapeutic effect comprises antibacterial effect and/or effect of stimulation, such as, increase the blood flow for the treatment of sites at the beginning, then reduces inflammation and produces collagen protein.Secondary light source is second group of LED104 in one embodiment, its emission spectra can activate be applied in above treatment sites or near treatment sites can photoactivatable composition, or topical blood flow to be regulated or collagen protein has reinvented impact.

Light activating agent can be generally included by photoactivatable composition, when it is activated, by treating wavelength and treating intensity emitting fluorescence, launching then can further in activated compositions or the energy of the light activating agent for the treatment of sites, and/or excite its molecule to exciting singlet, then, with other molecular reaction, produce reactive oxygen etc., thus for therapentic part provides therapeutic effect.It is believed that, the reactive oxygen of low content has therapeutic effect to tissue.Can the example of photoactivatable composition apply on November 9th, 2006 U.S. Patent Application Publication No.2007/0128132, the open No.WO/2010/051636 of PCT of application on November 6th, 2009, application on November 6th, 2009 the PCT application No.PCT/CA/2010/001134 to apply for 19, on July of the open No.WO/2010/051641 and 2010 of PCT in be described, the content of these patents is incorporated in this patent by reference.

Or what the emission spectra of the light that two kinds of light sources are launched all was applicable to that activation comprises one or more light activating agents can photoactivatable composition.Therefore, the activation energy of broader bandwidth, more effectively photoactivation light activating agent can be provided for light activating agent.Use incessantly a kind of there is the light activating agent of different absorption characteristic time, provide at least two kinds of light sources can these light activating agents of photoactivation.In addition, some light activating agents comprise multiple peak wavelength at its absorption spectrum.Therefore, provide two or more light source can in its whole absorption spectrum ranges complete activation light activator, thus from its photoactivation characteristic be benefited.

First group of LED102 and second group LED104 can provide complementary phototherapy process for therapentic part, thus pass through the therapeutic effect of second group of LED104, such as, can the therapeutic combination of photoactivation by activation, the therapeutic effect of first group of LED102 is enhanced and supplements.In certain embodiments, this can photoactivatable composition launch fluorescence be less than about 75mW/cm in the power density of skin surface ², be less than about 50mW/cm ², be less than about 10mW/cm ², be less than about 5mW/cm ², be less than about 2.5mW/cm ², or be less than about 2mW/cm ².Maximum power density can be about 0.02mW/cm ²to about 75mW/cm ², about 0.02mW/cm ²to about 50mW/cm ², about 0.02mW/cm ²to about 10mW/cm ², about 0.02mW/cm ²to about 5mW/cm ², or about 0.02mW/cm ²to about 10mW/cm ².

Now, with reference to an embodiment of figure 1 shown device 100, wherein first group of LED102 and second group LED104 is all placed in device head 106, and head can be connected by adjustable ground with the body 108 with base 110.Locking mechanism can be equipped with, for the position of locking head, base or body.LED can be arranged on (not shown) above the plate in lamp housing as array, from emitting surface 112 utilizing emitted light of head 106.Device 100 can comprise the wheel (not shown) that can lock at base position, thus device freely can be moved to the place of requirement by user, and is locked by setting position.

Optional is, according to power and the processing time of the LED adopted, in order to reduce heating, head 100 can comprise one or more cooling system (not shown), such as, air vent, Control device of liquid cooling etc. in radiator, one or more fan (not shown), lamp housing, for cooling first group and/or second group of LED.Cooling system can comprise the barrier absorbing heat.

Device also can comprise the controller 114 be connected with LED electrical, for controlling radiative various parameter, and such as, in opening/closing one group of LED single led and control different LED groups.Other parameter that controller can control has: the maximum power density of the light that each LED launches, bandwidth, peak emission wavelength, light emission time, utilizing emitted light power density with the change of light emission time, wavelength with the change of light emission time.Device operator are enable to require customization phototherapy processing scheme according to the treatment of each experimenter like this.

Head 106 can remove from the bearing of device 100, can change.In certain embodiments, for convenience's sake, lamp comprises tumbler (not shown), makes head 106 at any angle towards any direction, and can be locked in that position.In certain embodiments, lamp holder (106) can remove from device seat, uses as portable hand-held lamp.In this case, lamp holder can comprise one or more parts of controller and optionally can comprise power supply.In certain embodiments, provide a spacing probe (not shown), it extends on lamp holder 106, for detecting the distance of the surface of emission 112 and treatment sites.This can ensure the light transmitting suitable dose, the dosage of light and distance dependent.Spacing probe can be a kind of measuring device, and the ruler be such as of convenient length, maybe can comprise the sensing system utilizing sound or light detecting distance.

In certain embodiments, also provide a work surface, such as plate, basket or net, work surface is connected with the body of device 108, can remove.

The exemplary actual array space of first group of LED102 and second group LED104 is arranged as shown in Figure 2.Described array comprises 40 LED arranging layout by 8 row 5, and first group has 6 LED, and second group has 34 LED.Those of ordinary skill in the art will be appreciated that the LED of other quantity any of other array configurations any is also fine.In another embodiment, LED array comprises installation at least 36 LED onboard altogether, at least 40 LED, at least 46 LED or at least 184 LED.In one embodiment, LED array comprises 184 LED arranging layout by 8 row 23.In another embodiment, LED comprises 36 LED arranging layout by 6 row 6.Those of ordinary skill in the art will be appreciated that LED array can, with any deployment arrangements, can be any size or shape, such as rectangle, circle or other shape any.The surface of plate can be gradual change, thus some LED than other LED closer to treatment sites.Such as, can be rectangular slab, first surface comprise second group of LED, first group of LED installs, with first surface by certain spacing around first surface girth.

In the embodiment of fig. 2, first group of LED is different with the peak emission wavelength of second group of LED.The peak wavelength of first group of LED is about 410nm to about 420nm, bandwidth is about 13-15nm, and expect that it is to some antibacterial, such as propionibacterium acnes has antibacterial effect, the peak wavelength of second group of LED is about 440nm to about 470nm, and bandwidth is about 20nm ± 2nm.Fig. 3 is the exemplary emission spectra figure that this device embodiment measures at 5cm place, adopts SP-100 spectroradiometer (SP-100, ORBOptronix) to measure, to measure the relation of power density spectrogram and wavelength.Usually, total transmitting power density of this device embodiment 5cm distance is about 60 to 150mW/cm ², the gross energy launched for 5 minutes is about 20 to 50J/cm ².

In different embodiment (not shown)s, LED is arranged in flexible substrate, and flexible substrate forms dressing, veil or any other and can be administered to a part for skin, hair, fingernail or other structural material or be included within them.

Device head 106 can comprise multiple plate (not shown) that can connect, and LED installed by plate.Can be that any amount of plate links together, such as 2,3,4,5,6 or 7 blocks of plates.These plates can link together, thus its surface of emission 112 each other at an angle.Like this, can to the different side-irradiation light of the bending of experimenter or irregular treatment sites (such as face, arm or lower limb).The size and dimension of plate depends on pending position.Such as, process facial, can use single piece of bent plate, or is linked together by the plate that polylith is bending.Plate is connected to each other, but can move.There is provided a kind of locking mechanism, for plate being fixed on the position of expectation.

LED array can also comprise the 3rd group of LED, its peak wavelength of light launched or power density or bandwidth different from first group and/or second group of LED.In certain embodiments, the 3rd group of LED launches the light (such as, 630nm-700nm) being in visible electromagnetic spectrum red light portion.In other embodiments, the 3rd group of LED transmitting is in the light (such as, 635nm-590nm) that visible electromagnetic composes orange light part.In other embodiments, the 3rd group of LED launches the light (such as, 590nm-560nm) being in visible electromagnetic spectrum yellow part.In other embodiments, the 3rd group of light source launches the light (such as, 800nm-1000nm) being in electromagnetic spectrum infrared portions.The LED that alternate embodiment comprises radiative wavelength and power density is different from mentioned above, have different treatment effects.

Other exemplary array of first group of LED102 and second group LED104 as shown in Figure 4.The LED of second group of LED is dispersed between the LED of first group of LED.The ratio often organizing the quantity of LED and the quantity of both LED in first group of LED and second group LED can be different.The power density of launching due to different LED is different, can also according to the ratio of the quantity of the power density ratio definition LED of first group of LED and second group LED.Can require that the therapeutic effect realized customizes quantity or power density ratio according to device 100.Such as, for the skin infected, the relative power that first group of LED launches can be increased, and for cosmetic treatments, the relative power that second group of LED launches can be increased.

In certain embodiments, LED is equidistant placement.Spacing distance on plate between each LED can be any distance, such as, and 4 to 7mm, about 4 to 10mm, about 10 to 15mm, or about 15 to 20mm.In certain embodiments, LED at the about 10-12mm in interval, a direction, at the about 13-18mm in another interval, direction.If claimed apparatus 100 provides higher power, LED can arrange tightr.In some other embodiment, LED is not equidistant placement.

Device 100 optionally comprises dismountable veil (lid), to reduce the size of head 100 launch site.Device can be configured for transmission light, makes light in use or does not use the exposed surface of veil to be substantially uniformly distributed.Head can be the suitable shape of U-shape, circle or other applicable lamp holder any.Device 100 also can comprise wave filter, for filtering the light that emitting surface 112 is launched, thus launches the light of suitable wavelength, bandwidth or power density.Wave filter can comprise ultraviolet filter, collimator, reflector or adjusting device 100 other means any of utilizing emitted light shape.

Fig. 5 is the emission spectra of the different embodiment of device, measuring method is identical with Fig. 3, the place different from embodiment mentioned above is, the peak wavelength of second group of LED is higher, about 450 to about 480nm, head comprises three blocks of plates, is surrounded with the body part of profile, a part for such as face, arm and lower limb or whole part.In this embodiment, the average power density processing distance is less than about 75mW/cm ², or about 30 to 150mW/cm ².

In another embodiment of device, the peak wavelength of the light of the first light source is about 430 to about 500nm, about 440 to about 500nm, about 450 to about 500nm, about 430 to about 475nm, approximately 435nm to about 470nm, approximately 440nm to about 460nm, approximately 445nm to about 455nm, approximately 440nm, approximately 450nm, approximately 460nm or about 470nm.

The peak wavelength of the light of secondary light source is about 400nm to about 500nm, approximately 400nm to about 475nm, approximately 400nm to about 450nm, approximately 400nm to about 430nm, or approximately 410nm to about 420nm or approximately 415nm.

Or, second peak wavelength can be about 480 to about 760nm, approximately 480nm to about 700nm, approximately 480nm to about 650nm, approximately 480nm to about 620nm, about 500 to about 700nm, approximately 520nm to about 700nm, approximately 500nm to about 660nm, approximately 540nm to about 640nm, approximately 540nm to about 580nm, approximately 500nm to about 570nm, approximately 570nm to about 590nm, approximately 590nm to about 610nm, or about 610nm to about 760nm.In certain embodiments, compared with the light of the first light source, the light of secondary light source has the average power density that wider bandwidth sum is lower.

Another embodiment of device is with the difference of embodiment above, it is not the first light source and the secondary light source that adopt the light launching different peak wavelength, but providing at least one to launch the light source of certain light (such as white light), this light is converted to required spectrum by one or more wave filter.At least one light source described is the light source of LED array or other transmitting white.Wave filter comprises coloured lamella lucida, the lamella lucida that such as stage illumination, lighting uses, and can eliminate unwanted light, only allows useful light arrive treatment sites.In another embodiment, wave filter can comprise a kind of fluorescent agent.

With reference now to controller 114, by controller, different process parameters can be set in advance as a kind of tupe, or can be customized by device operator.Such as, controller 114 enables user select specific peak wavelength, such as HONGGUANG, gold-tinted, blue light and/or infrared light wavelength, or their combination, processes different situations, such as dermatosis or wound.Also the light of other color type can be used.Like this, according to the peak wavelength expected, device comprises different LED groups.Controller 114 can optionally comprise display screen (not shown), and assisting users is selected and control treatment pattern, intervalometer and other functional characteristics.

Controller 114 can or be opened within the different time simultaneously has different emission spectra, the different LED group of such as peak wavelength, or other light source.May need to activate different light sources simultaneously, thus obtain the radiation response of different light sources simultaneously.In addition, the combination of two or more emission spectra can bring cooperative effect, makes treatment effect than once adopting any one emission spectra single administration more effective.Or, may need once to activate a kind of emission spectra.If expection operates several emission spectra and can't produce cooperative effect simultaneously, if or determined to do like this there is ill effect, then in single emission spectra, operate phototherapy lamp more satisfactory.May also need to be used alternatingly between two or more emission spectra.Such as, in the weekly treatment of acne, being used alternatingly two kinds of wave-length coverages (such as 633nm (HONGGUANG) and 415nm (purple light)) can be more effective than being used alone the treatment of a kind of wave-length coverage.

Tupe can store on a storage device with machine readable form (description as relevant to the device in Fig. 6) or in machine data storehouse.Such as, user can select one or more in need dermatosis to be processed or disease inventory.When tupe is stored in memory device or data base, lamp controller can read the operating parameter treating the phototherapy lamp corresponding to processing with concrete lamp.These parameters can comprise dosage (defined by processing time, mean power, process distance, peak wavelength and bandwidth), can be inputted by device manufacturer or programmer, also can carry out adjustment operation parameter by user according to the phototherapy handling procedure of customization.

The circuit of device comprises the switch for select operating mode.Switch can be implemented in hardware, software, firmware or their combination.Different operator schemes specifies the different parameters of produced light, such as wave-length coverage, bandwidth, peak wavelength or other light source parameters any.In addition, light source can configure, and produces light pulse to allow it.If light-pulse generator, identical energy to be transmitted within the time identical with non-pulse light source, need to adopt higher light intensity.Therefore, may need to adopt the light that burst transmissions intensity is higher in the short time, to improve therapeutic efficiency.Now, different patterns can comprise different pulse parameters, such as pulse duration, pulse frequency, pulse strength, number of pulses or other pulse parameter any.In some cases, may need to keep pulse parameter constant during whole process, thus repeatedly transmit identical pulse.In other cases, the one or more pulse parameters changing several pulse in same period in time may be needed.Such as, may need to operate according to the pattern of " pulse strength increases with every subpulse ".In another example, may require that each pulse replaces between two or more wave-length coverage.The parameter (non-pulse or pulse) of the light produced can regulate, to realize any amount of pattern.

Different operation modes can also be included in different time and irradiate different lamp source groups, such as LED.Such as, in a kind of pattern, only irradiate wherein one group of LED, or select the LED of quantity in irradiating one group.If need position to be processed (such as wound) less, thus open all LED illumination the position of process can be made to be greater than requirement, therefore, above-mentioned way is more satisfactory.For example, the skin processing ocular vicinity position may be needed, but, may cause damage to eyes transmission light.In this case, adopt a light source subgroup, thus control the size and dimension at pending position suitably, will be helpful.In another example, when a kind of pattern comprise use pulse time, two groups of light source subgroups alternately can open and close pulse, thus at any given time in only have one group of light source irradiating.When expecting to treatment sites transmission high strength transient state light, this pattern can be selected.

Device operator can directly select a kind of operator scheme, or device can comprise user interface, allow user to select one or more target, and device can configure, to select suitable pattern according to the selection of user.Such as, in user interface, device operator can represent that it expects to adopt a kind of pattern to carry out Acne treatment, a kind of suitable pattern can be selected, such as, the first transmitting maximum peak when non-pulse transmitting, approximately 14nm full width at half maximum bandwidth is about 400nm to about 430nm, second maximum peak is about 440 to about 470nm, or other suitable acne treatment pattern any.

Can be met in scope that any national legislation requires carry out suitable adjustment in safety by device circuit or the pattern selected by operator.

In some embodiments, device comprises the sensor and feedback mechanism that communicate with controller, thus the various parameters that the output of device and sensor detect are associated.Sensor can be light, heat or biometric sensors etc.Such as, if the heat sensor temperature sensed near treatment sites or treatment sites has reached or close to reaching predetermined temperature, device is by radiative for corresponding adjustment parameter, such as, reduce power density by (i) and extend the processing time, to transmit same dose, (ii) dosage is reduced, (iii) shutoff device, (iv) warning is started, v () starts cooling system (if having cooling system), around cooling processing position or treatment sites inside, or (vi) regulates the output of cooling system (if having cooling system), such as, cooldown rate or cooling degree.

Sensor can be optical pickocff, for checkout gear irradiate can the light output of photoactivatable composition, for the utilizing emitted light detected, device is passable, such as (i) when can photoactivatable composition be finished (photobleaching) time close lamp, (ii) regulate the emission spectra of light irradiating compositions, with the change of the spacing of device and compositions, or the thickness of compositions is relevant.This can be considered as self-regulatory mechanism, changes the feature of lamp according to the transmitting of photoactivatable composition.

In certain embodiments, sensor is used for photoactivatable composition and device matching.Such as, lamp only just starts when it " sees " certain optical signals from light activatable compositions, or just starts when it " sees " particular bar from composition container.This also can be regarded as a safety feature.

Fig. 6 can comprise in said device, for performing the block diagram of the computing equipment of any process of the present invention.Each parts of these systems can be implemented in one or more computing equipment 400.In some aspects, multiple parts of these systems can be included in a computing equipment 400.In some implementations, parts and storage facilities can be implemented in several computing equipment 400.

Computing equipment 400 comprises at least one communications interface unit, i/o controller 410, a system storage, and one or more data storage device.System storage comprises at least one random access memory (RAM402) and at least one read only memory (ROM404).All these elements all communicate with CPU (CPU406), facilitate the operation of computing equipment 400.Computing equipment 400 can configure by many different modes.Such as, computing equipment 400 can be traditional stand-alone computer, or the function of computing equipment 400 can be distributed in several department of computer science and unify in framework.In the diagram, computing equipment 400 is connected with other server or system by network or localized network.

Computing equipment 400 can configure by distributed structure/architecture, and wherein data base and processor are placed in independently in unit or place.Some unit performs main processing capacity, comprises at least one general purpose controller or processor and system storage.In distributed structure/architecture is implemented, each unit of these unit can be connected with communication hub or port (not shown) by communications interface unit 408, and communication hub or port are used as to be connected with the Primary communication of other server, client or subscriber computer and Other related equipment.Communication hub or port itself can have minimum disposal ability, are mainly used as communications router.Various communication protocol can be a part for system, include, but are not limited to following these: Ethernet, SAP, SAS ^tM, ATP, BLUETOOTH ^tM, GSM and TCP/IP.

CPU406 comprises processor, such as one or more conventional microprocessor and one or more supplementary coprocessor, and such as math co-processor, for unloading the workload of CPU406.CPU406 communicates with communications interface unit 408 and i/o controller 410, and by them, CPU406 communicates with miscellaneous equipment (such as other server, user terminal or equipment).Communications interface unit 408 and i/o controller 410 can comprise multiple communication port, for communicating with such as other processor, server or client simultaneously.

CPU406 also communicates with data storage device.Data storage device can comprise the appropriately combined of magnetic, light or semiconductor memory, also can comprise, such as, and RAM402, ROM404, flash disc drives, CD (such as fine groove disk) or hard disk or driver.CPU406 and data storage device passable, such as, entirety is positioned within single computer or other computing equipment; Such as, or by telecommunication media, USB port, serial-port cables, coaxial cable, Ethernet cable, telephone wire, RF transceiver or other similar wireless or cable medium or more combination are connected to each other.Such as, CPU406 can be connected with data storage device by communications interface unit 408.CPU406 can configure and perform one or more concrete processing capacity.

Data storage device can store, such as, and the operating system 412 of (i) computing equipment 400; (ii) one or more application 414, (such as, computer program code or computer program), is applicable to according to system and method for the present invention, particularly according to the process wizard CPU406 described in detail when relating to CPU406; Or (iii) is adjusted to be applicable to the data base 416 for storage information, for the information of storage program.

Operating system 412 and application 414 with such as compressing, not compiling and the form such as encryption stores, can comprise computer program code.The instruction of program can be read in the main storage of processor from the computer-readable media (such as from ROM404 or from RAM402) except data storage device.When in program, the execution of job sequence causes CPU406 to perform program step of the present invention, hard-wired circuitry can be used for replacing software instruction, or is combined with software instruction, for implementing program of the present invention.Therefore, system and method for the present invention is not limited to the concrete combination of any hardware and software.

Suitable computer program code can being provided, relating to for performing one or more function selecting operator scheme of the present invention.Program also can comprise program unit, such as operating system 412, data base management system and " device driver ", make processor be connected with computer peripheral equipment (such as video display units, keyboard, computer mouse etc.) by i/o controller 410.

Word " computer-readable media " refers to any non-transitory media, and described media provide to the processor (or other processor any of device of the present invention) of computing equipment 400 or participate in providing instruction.This media can have many forms, include but not limited to non-volatile media and volatile media.Non-volatile media comprises CD, disk or light-disk, or integrated circuit storage (such as flash memory) etc.Volatile media comprises the dynamic random access memory (DRAM) usually forming main storage.The common form of computer-readable media comprises, such as, floppy disk, floppy disc, hard disk, tape, other magnetic medium any, CD-ROM, DVD, other optical media any, punched card, paper tape, other physical medium with holes any, RAM, PROM, EPROM or EEPROM (EEPROM), FLASH-EEPROM, other memory chip any or tape, or the non-transitory media of other embodied on computer readable any.

The various forms of computer-readable media can to relate to one or more sequence transmission of one or more instructions to CPU406 (or other processor any of device of the present invention) to perform.Such as, instruction can be stored on the disk of remote computer (not shown) at the beginning.Instruction can be loaded in its dynamic memory by remote computer, and connected by Ethernet, cable or even utilize modem to send instruction by telephone wire.The local communication device (such as server) of computing equipment 400 can receive the data on each communication line, and data is placed on the system bus of processor.System bus transfers data to main storage, and processor is undertaken retrieving and performing instruction by main storage.The instruction that main storage receives can optionally be stored in memorizer before or after execution by a processor.In addition, instruction can be received with electricity, electromagnetic or optical signal by COM1, and these signals transmit the radio communication of various information type or the example forms of data flow.

In certain embodiments, device can be implemented or be integrated in existing article (such as mobile device), such as hand-held computing device, smart phone, or band display screen or flash lamp are as the mobile phone of emitting surface, such as samsung such as, such as, or desk computer, TV, bathtub, shower, sun tan bed, health overcover, blanket can be implemented or be integrated into device, clothes article, in the display screen of robe, glove etc.Method

The invention also discloses process subjects skin, wound, damage or other dermopathic method.Light to the treatment benefit of subjects skin and wavelength radiative in the processing time and power density and total transmitting power relevant.

In certain embodiments, described method comprises with the about 4mW/cm of average power density ²to about 150mW/cm ²illumination penetrate the skin of experimenter.In certain embodiments, when adopting the LED of two or more sets different wavelength range, the power density of one group of LED is restricted to lower than the threshold value of another group LED.Such as, the power density of one group of LED is restricted to and is less than 10% of another group LED power density, or is less than other suitable threshold value any.

In other respects, described method comprise adopt the first illumination to penetrate to be applied in above organized processing position or neighbouring can photoactivatable composition, or irradiate organized processing position, the first light has can to can the first peak wavelength of activating of photoactivatable composition; With the second illumination penetrate same or different can photoactivatable composition or irradiate the treatment sites of described tissue, the second light has the second peak wavelength, second peak wavelength is different from the first peak wavelength, and wherein the first and second peak wavelengths are all in blue light and/or the purple light district of electromagnetic spectrum.

Other aspects, described method comprises: adopt the first illumination with the first peak wavelength to penetrate described tissue; Adopt the second illumination to penetrate described tissue, the second light has second peak wavelength different from the first peak wavelength, and wherein the first peak wavelength is in blue light and/or the purple light district of electromagnetic spectrum.

Other aspects, described method comprises employing peak wavelength about 400 to about 750nm, and power density about 4 is to about 75mW/cm ², or about 55mW/cm ²to about 150mW/cm ²the first illumination penetrate tissue.

Other aspects, described method comprises employing peak wavelength about 400 to about 750nm, and tissue is penetrated in the first illumination of the about 19nm of bandwidth ± about 5nm.

Other aspects, described method comprises employing peak wavelength about 400 to about 750nm, and the about 15nm of bandwidth is about 100nm, approximately 25nm to about 80nm, approximately 30nm extremely about 70nm extremely, or tissue is penetrated in the first illumination of about 20nm to about 50nm.

Other aspects, described method comprises and adopts peak wavelength about 400 to the first illumination of about 750nm to penetrate tissue, regulates at least one parameter in the peak wavelength of the first light, bandwidth, power density or energy density during irradiated tissue.

Other aspects, described method comprises and adopts peak wavelength about 400 to the first illumination of about 750nm to penetrate tissue, and during single treatment, energy density is about 4 to about 60J/cm ², about 10 to about 60J/cm ², about 10 to about 50J/cm ², about 10 to about 40J/cm ², about 10 to about 30J/cm ², about 20 to about 40J/cm ², about 15J/cm ²to 25J/cm ², about 10 to about 20J/cm ².

In certain embodiments, light is applied to therapentic part 1 second to 30 minute.In certain embodiments, the time of application of light is 1-30 second, 15-45 second, 30-60 second, 0.75-1.5 minute, 1-2 minute, 1.5-2.5 minute, 2-3 minute, 2.5-3.5 minute, 3-4 minute, 3.5-4.5 minute, 4-5 minute, 4-6 minute, 5-7 minute, 6-8 minute, 7-9 minute, 8-10 minute, 9-11 minute, 10-12 minute, 11-13 minute, 12-14 minute, 13-15 minute, 14-16 minute, 15-17 minute, 16-18 minute, 17-19 minute, 18-20 minute, 19-21 minute, 20-22 minute, 21-23 minute, 22-24 minute, 23-25 minute, 24-26 minute, 25-27 minute, 26-28 minute, 27-29 minute, or 28-30 minute.Processing time can be no more than about 90 minutes, about 80 minutes, about 70 minutes, about 60 minutes, about 50 minutes, about 40 minutes or about 30 minutes.Processing time depends on total joule of number of the luminous energy being transferred to treatment sites, and therefore, radiative power density is higher, and required time is shorter, and vice versa.

In certain embodiments, the time of application of light is no more than about 90 minutes, about 80 minutes, about 70 minutes, about 60 minutes, about 50 minutes, about 40 minutes or about 30 minutes, about 1 minute to about 30 minutes, about 1 minute to about 25 minutes, about 1 minute to about 20 minutes, about 1 minute to about 19 minutes, about 1 minute to about 18 minutes, about 1 minute to about 17 minutes, about 1 minute to about 16 minutes, about 1 minute to about 15 minutes, about 1 minute to about 14 minutes, about 1 minute to about 13 minutes, about 1 minute to about 12 minutes, about 1 minute to about 11 minutes, about 1 minute to about 10 minutes, about 1 minute to about 9 minutes, about 1 minute to about 8 minutes, about 1 minute to about 7 minutes, about 1 minute to about 6 minutes, about 1 minute to about 5 minutes, about 1 minute to about 4 minutes, about 1 minute to about 3 minutes.

In certain embodiments, the method of process experimenter's tissue (such as skin, wound, damage or other dermatosis) is first used to tissue before being included in the light irradiating a certain wavelength and power density (such as, apparatus of the present invention 100 embodiment) further can the compositions of photoactivation.

Describedly can comprise one or more compositionss by photoactivatable composition.Such as, describedly the light activating agent component (" light activating agent ") that can be activated by the light of specific wavelength (i.e. actinic light) can be comprised by photoactivatable composition.Light activating agent component comprise one or more by actinic light activation and can accelerate luminous energy dispersion light activating agent molecule, cause light activating agent itself to have therapeutic effect, or cause other reagent (when there is this compound in compositions) with therapeutic effect (such as accelerating the catabolic process of the oxidant of peroxide and so on) comprised in compositions that photochemical activation occurs.Included light activating agent irradiates with the photon of certain wavelength, and can be excited to higher energy state.When the excitation electron of light activating agent turns back to lower energy state, they release the lower photon of energy level, thus launch the longer light of wavelength (Stokes shift).In suitable environment, many energy be transferred to can photoactivatable composition other composition or be directly transferred to therapentic part.

Suitable light activating agent can be fluorescent dye (or coloring agent), although also can use other dye set or dyestuff (biology and histology's dyestuff, food coloring, carotenoid).Increase light absorption in conjunction with light activating agent by combination dye molecule, and improve the selectivity of absorption and light-biological regulation.Energy also can be caused to transmit between light activating agent in conjunction with light activating agent.This just creates several probabilities producing new heliosensitivity and/or selective light activating mixtures.Suitable light activating agent comprises:

Chlorophyll dyestuff

Exemplary chlorophyll dyestuff includes but not limited to chlorophyll a; Chlorophyll b; The molten chlorophyll of oil; Bacteriochlorophyll a; Bacteriochlorophyll b; Bacteriochlorophyll c; Bacteriochlorophyll d; Protochlorophyll; Protochlorophyll a; Amphiphilic phyllins 1; Amphiphilic phyllins 2.

Xanthene derivant

Exemplary xanthene dye includes but not limited to Eosin B (bromo-2', the 7'-dinitro-fluorescein dianion of 4', 5'-bis-); Eosin W or W S; Eosin W or W S (the bromo-fluorescein dianion of 2', 4', 5', 7'-tetra-); Eosin (the bromo-fluorescein dianion of 2', 4', 5', 7'-tetra-); Eosin (the bromo-fluorescein dianion of 2', 4', 5', 7'-tetra-) methyl ester; Eosin (the bromo-fluorescein monovalent anion of 2', 4', 5', 7'-tetra-) p-isopropyl benzyl ester; Eosin derivatives (the bromo-fluorescein dianion of 2', 7'-bis-); Eosin derivatives (the bromo-fluorescein dianion of 4', 5'-bis-); Eosin derivatives (the chloro-fluorescein dianion of 2', 7'-bis-); Eosin derivatives (the chloro-fluorescein dianion of 4', 5'-bis-); Eosin derivatives (the iodo-fluorescein dianion of 2', 7'-bis-); Eosin derivatives (the iodo-fluorescein dianion of 4', 5'-bis-); Eosin derivatives (tribromo fluorescein dianion); Eosin derivatives (2', 4', 5', 7'-tetrachloro-fluorescein dianion); Eosin; Eosin dicetyl yl pyridines chloride ion pair; Erythrosin B (the iodo-fluorescein dianion of 2', 4', 5', 7'-tetra-); Erythrosine; Erythrosine dianion; Erythrosin B; Fluorescein; Fluorescein dianion; Phloxin B (bromo-3,4,5, the 6-tetrachloro-fluorescein dianions of 2', 4', 5', 7'-tetra-); Phloxin B (four chloro-tetrabromo-fluoresceins); Phloxin B; Rose-red (3,4,5,6-tetra-chloro-2', 4', 5', 7'-tetraiodofluorescein dianion); Pyronine G, pyronine J, pyronine Y; Rhodamine, as rhodamine comprises the bromo-rhodamine methyl ester of 4,5-bis-; The positive butyl ester of the bromo-rhodamine of 4,5-bis-; Rhodamine 101 methyl ester; Rhodamine 123; Rhodamine 6G; The own ester of rhodamine 6G; Four bromo-Rhodamine 123s; And tetramethyl-rhodamine ethyl ester.

Methylene blue dye

Exemplary methylene blue derivatives thing includes but not limited to that 1-methylmethylene is blue; 1,9-Dimethylmethylene blue; Methylene blue; Methylene blue (16.mu.M); Methylene blue (14.mu.M); Methylene-violet; Bromine methylene violet; 4-iodine methylene violet; 1,9-dimethyl-3-dimethyl-amino-7-diethyl-amino-phenothiazine; And 1,9-dimethyl-3-diethylamino-7-dibutyl-amino-phenothiazine.

Azo dye

Exemplary azo (or two azos-) dyestuff includes but not limited to crystal violet, dimethyl diaminophenazine chloride, para red (paratonere 1), amaranth (Azorubin S), Xylene Red (azorubine, Food Red 3, azogeramine 4), lures red AC (FD & C40), tartrazines (FD & C Huang 5), orange G (acid orange 10), ponceau 4R (Food Red 7), C.I. 13020. (Xylene Red 2) and murexide-ammonium purpurate.

Of the present invention in some, one or more light activating agent can independently selected from following arbitrary chromophore: acid black 1, acid blue 22, acid blue 93, acid fuchsin, acid green, acid green 1, acid green 5, acetocarmine, acid orange 10, Acid red 26, Xylene Red 29, Xylene Red 44, CI 45430, Xylene Red 66, acid red 87, eosin scarlet, acid red 92, Xylene Red 94, azogeramine 01, azogeramine 03, acid fuchsin, acid fuchsin, acid violet 19, Indian yellow 1, Indian yellow 9, acid yellow 23, Indian yellow 24, quinoline yellow 6, Indian yellow 73, Indian yellow S, acridine orange, acriflavinium chloride, alcian blue, Alcian yellow, the molten eosin of alcohol, alizarin, alizarin blue 2RC, alizarin-carmine, alizarin cyanine BBS, alizarin cyanine R, alizarin red S, alizarin purple, aluminon, amide black 10b, amino black, aniline blue WS, anthracene blue SWR, auramine O, AzocannineB, azocarmine G, azo diazonium (Azoicdiazo) 5, azo diazonium 48, azure A, aB, aC, alkali blue 8, alkali blue 9, alkali blue 12, alkali blue 15, alkali blue 17, alkali blue 20, alkali blue 26, bismarck brown 1, basic fuchsin, Viride Nitens 4, Basic Orange 14, alkalescence red 2, alkalescence red 5, alkalescence red 9, alkalescence purple 2, alkaline purple 3, alkalescence purple 4, alkaline purple 10, basic violet 14, basic yellow 1, basic yellow 2, biebrich red, bismark brown Y, the scarlet 6R of bright crystallization, calred, carmine, carminic acid (acid 4), celestine blue B, Chinese blue, dried female insect, Coelestine is blue, chrome violet CG, Chromotrope 2R, Chromoxane cyanine R, the Congo corinth, Congo red, cotton blue, cotton red, Croceine is scarlet, crocin, crystal ponceau 6R, crystal violet, large beautiful purple, diamond green B, direct blue 14, sun blue 58, directly red, directly red 10, directly red 28, directly red 80, direct yellow 7, Eosin B, blue eosin, eosin, eosin W or W S, yellowish eosin, Eosinol, erie garnet B, eriochrome cyanine R, Erythrosin B, ethyl eosin, ethyl green, ethyl violet, azovan blue, fast blue B, fast green FCF, Fast Red B, fast yellow, fluorescein, Food Green 3, pyrogaelol phthalein, gallamine is blue, Gallocyanine, Gentian Violet, hematein, hematoxylin, haematoxylin, the strong pinkish red BBL of daylight, first is blue, hematein, hematoxylin, haematoxylin, hoffmann's violet, imperial red, indocyanine green, A Li Xinlan, A Li Xinlan 1, A Lixin Huang 1, INT, fuchsin, kermesic acid, Kernechtrot, lac, lac acid, Lauth's violet, pale green, Lissamine green SF, Luxol fast blue, pinkish red 0, pinkish red I, pinkish red II, pinkish red III, peacock green, manchester brown, Manchester yellow, merbromin, mercurochrome, metanil yellow, methylene azure A, methylene aB, methylene aC, methylene blue, methylene is green, crystal violet, methyl violet 2B, crystal violet 10B, mordant dyeing indigo plant 3, mordant dyeing indigo plant 10, mordant dyeing indigo plant 14, mordant dyeing indigo plant 23, mordant dyeing indigo plant 32, mordant dyeing indigo plant 45, mordant rouge 3, mordant rouge 11, mordant dyeing purple 25, mordant dyeing purple 39, naphthol blue black, naphthol green B, Citronin A, natural black 1, neutral red, neutral red 3, neutral red 4, neutral red 8, neutral red 16, neutral red 25, neutral red 28, natural yellow 6, NBT, neutral red, New Fuchsine, Niagara blue 3B, night is blue, Nile blue, Nile blue A, Nile red, nile blue sulfate, Nile red, nitro BT, nitroblue tetrazolium, Kernechrot, oil red O, orange G, orcein, paramagenta, phloxine B, picric acid, ponceau 2R, Ponceaux 6R, ponceau B, xylidine ponceau, Ponceau S, Flos Primulae Vittatae, alizarinopurpurin, phycocyanobilin, rhodophyll.Phycoerythrocyanin (pec) (PEC), phthalocyanine, pyronine B, pyronine G, pyronine Y, rhodamine B, rosaniline, rose-red, safranine, Safranin O, scarlet R, scarlet, scarlet R, lac, sirius red F3B, solochrome cyanina R, soluble blue, solvent black 3, solvent blue 38, solvent red 23, solvent red 24, solvent red 27, solvent red 45, solvent yellow 94, the molten eosin of alcohol, soudan III, Sudan IV, Sudan black B, sulfur S, Swiss blue, tartrazines, thioflavine S, thioflavine T, 3,7-Diaminophenothiazine., toluidine blue, toluidine red, aniline yellow G, acriflavinium chloride, trypan blue, fluorescein sodium, victoria blue 4R, victoria blue B, victoria green B, water blue I, water-soluble eosin, xylidine ponceau or yellowish eosin.

Can comprise other compound by photoactivatable composition, such as oxygen-rich agent, containing oxygen reagent, pH controlling agent (such as sodium acetate, sodium hydroxide), optical diffraction agent (such as porcelain crystal, hydroxyapatite), healing factor (such as hyaluronic acid, glycosamine), chelating agen (such as EDTA, EGTA), steatolysis stimulant (such as caffeine), and/or hydrophilic gellant (such as glucose, cellulose).

When in time can using together with photoactivatable composition, described device is particularly useful in a more than peak wavelength luminescence.Such as, when the light source is an led, described device comprises the LED of a more than type, and each LED is luminous at different wavelength.Such as, each LED can emission wavelength with can the overlapping or light that mates of the absorption band of one or more chromophore in photoactivatable composition.The LED of every type can open and close separately.

In certain embodiments, described method comprises that i) use to subjects skin can photoactivatable composition, ii) illumination wavelength with use can the overlapping light of the absorption spectrum of photoactivatable composition, wherein the irradiation time of light is until can there is significant photobleaching by photoactivatable composition.

In certain embodiments, described method comprises that i) use to subjects skin can photoactivatable composition, ii) illumination wavelength with use can overlapping the first light of the absorption spectrum of photoactivatable composition, wherein the irradiation time of the first light is until can there is significant photobleaching by photoactivatable composition, and iii) the second light that illumination wavelength is different from the first light.In the particular embodiment, described method comprises that i) use to subjects skin can photoactivatable composition, wherein can photoactivation compositions absorb visible electromagnetic spectrum blue light components light, ii) to the skin exposure blue light of experimenter, until significant photobleaching can occur photoactivatable composition, and iii) execute according to HONGGUANG to subjects skin.

In certain embodiments, described method comprises and adopts peak wavelength about 400 to the first illumination of about 750nm to penetrate tissue, regulates at least one parameter in the peak wavelength of the first light, bandwidth, power density or energy density during irradiated tissue.In one embodiment, described method is included in illumination and penetrates the maximum power density that the light that at least one light source is launched is reduced or improved to period.The light of Different Light can carry out different adjustments in the different time or simultaneously.Will be appreciated that and may only regulate the light of a light source within the time of a total irradiation time part, or all regulate in whole irradiation time.In certain embodiments, power density can raise by any speed or decline, and such as, speed often irradiates a minute about 0.002mW/cm ²to often irradiating a minute about 0.1mW/cm ², about 0.005mW/cm ²/ minute, about 0.006mW/cm ²/ minute, or about 0.012mW/cm ²/ minute.

In certain embodiments, the maximum power density irradiating light is about 0.01mW/cm ²to about 200mW/cm ², 0.02mW/cm ²to about 150mW/cm ², 0.02mW/cm ²to about 135mW/cm ², 0.02mW/cm ²to about 75mW/cm ², 0.02mW/cm ²to about 60mW/cm ², about 0.02mW/cm ²to about 50mW/cm ²about 0.02mW/cm ²to about 30mW/cm ², about 0.02mW/cm ²to about 15mW/cm ².

In some other embodiment, the only pulse that LED array or other light source produce.In certain embodiments, the pulse duration of the light of generation is about 10ms to about 300ms.But, according to application, the longer and shorter pulse duration can be adopted.In certain embodiments, the pulse duration of the light of generation is about 20ms to about 100ms.In certain embodiments, the pulse duration of the light of generation is about 20ms to about 60ms.In certain embodiments, the pulse duration of illumination beam is about 20ms to about 40ms.In certain embodiments, the pulse duration of the light of generation is about 40ms to about 60ms.In certain embodiments, the pulse duration of the light of generation is about 40ms.In certain embodiments, the pulse duration of the light of generation is greater than about 40ms.In certain embodiments, the pulse duration of the light of generation is less than 1ms, is preferably less than 500ns.In addition, the pulse duration depends on other feature of the light of generation, such as amplitude, wavelength, bandwidth or their combination.

Before the light that described method also can be included in transmitting arrives tissue site (such as skin), filtered by one or more optical element, decay, amplify, polarization or change it.Such as, certain wave filter can eliminating the light of a certain bandwidth can be adopted to filter (such as ultraviolet filter) light.Can also calibrate light.

In certain embodiments, phototherapy device also can with can use together with the barrier of light launched of the LED of effective baffling device.Such as, when only a part of skin surface needs to process experimenter, barrier can be adopted to prevent the illumination of launching to be mapped to and not need skin part to be processed.

Purposes

System of the present invention, apparatus and method make phototherapy technology can be used in various beauty treatment, health and medical application.It has been found that, it is useful that the cytoactive light of being induced by light regulates in skin treating or processing method.System of the present invention, apparatus and method can be used for Wound healing and bone regeneration and tissue repair, skin condition, skin renewal, skin care and acute inflammation.

" skin renewal " refer to minimizing, eliminate, delay or the process of one or more symptoms that reversing skin is aging.Such as, the common sympton of skin aging includes but not limited to occur microgroove or wrinkle, skin becomes thin and transparent, subcutaneous fat runs off (cause cheek sink, eye socket is deeply recessed and hand is obviously lost with cervical region compactness), osteoporosis (thus be separated with skin because osteoporosis causes skeleton to shrink, cause Dermatochalasia), xerosis cutis (may itch), antiperspirant cannot all flow out and cool skin, face becomes mildewed, freckle, senile plaque, spider-like vein, pachylosis and keratin skin, microgroove, Dermatochalasia or the long speckle that can disappear when pulling open.One or more symptoms of skin aging mentioned above can be reduced, eliminate, delay or even be reversed by device of the present invention, method, purposes and system.

" dermatosis " includes but not limited to erythema, telangiectasis, photochemical telangiectasis, psoriasis, skin carcinoma, pemphigus, sunburn, dermatitis, actinic keratosis, eczema, acne, measles, impetigo, lichen chronicus simplex, hypertrophic rosacea, Perioral Dermatitis, diffusibility sebaceous hyperplasia, other steatosis, collagen protein-Related Skin Diseases (connective tissue disease), other sweat gland disease, granuloma dermatosis, vascular lesion, optimum pigmentosa pathological changes, hair disease and some skin infection, slow life and acute inflammation, pseudofolliculitis barbae, drug eruption, erythema multiforme, erythema nodosum, granuloma annulare, actinic keratosis, purpura, speckle is bald, aphthous stomatitis, dermatitis medicamentosa, dry skin, neoplastic disease, chap, axersis, ichthyosis vulgaris, fungal infection, herpes simplex, intertrigo, scar tumor, seborrheic keratosis, milium, molluscum contagiosum, pityriasis rosea, pruritus, urticaria and hemangioma and various deformity.Dermatitis comprises contact dermatitis, atopic dermatitis, seborrheic dermatitis, nummular dermatitis, whole body exfoliative dermatitis and nature static dermatitis.Skin carcinoma comprises melanoma, basal cell carcinoma and squamous cell carcinoma.

Such as, some types of acne, comprise acne vulgaris, acne cystica, acne atrophica, bromine acne, chloracne, acne conglobata, esthetics acne, acne detergicans, popular acne, epidemic acne, acne estivalis, acne fulminans, acne halogen, sclerancne, iodine acne, acne cheloidique, acne mechanica, acne papulosa, pomade acne, menstruation front stall skin ulcer, pustular acne, acne scorbutica, acne scrofulosorum, acne urticata, acne varioliformis, acne venenata, propanoic acid acne, acne artificialis, Gram-negative acne, steroid acne and tuberosity acne cystica.

Some dermatosis has different symptoms, comprise rubescent, redden, causalgia, desquamation, pimple, pimple, pustule, acne, speckle, joint knot, capsule, blister, telangiectasis, spider-like vein, skin ulcer, surface stimulation or pain, itch, inflammation, red, purple or locus coeruleus or variable color, nevus and/or tumor.

" wound " refers to the damage of any tissue, comprise acute, subacute, unresolved or be difficult to heal wound and chronic wounds etc.The example of wound comprises open wound and closure wound.Wound comprises burn, otch, excision, lacerated wound, wear and tear, stab or penetrating wound, surgical wound, scratch, hematoma, weigh wounded, wound (periodontal inflammation) that skin ulcer (such as decubital ulcer), ulcer, periodontitis cause etc.Ulcer comprises diabetic foot ulcers, pressure ulcer, amputation, varicose ulcer, chronic ulcer and/or is classified as any wound of I level to IV level wound.

" acute inflammation " show as pain, heating, rubescent, swelling and loss of function.It comprises those symptoms allergic that insect bite etc. causes, and as mosquito, Apis, wasp bites, poison ivy is poisoning, rear ablation etc.

According to instruction of the present invention, identify that suitable device, method and purposes are that those skilled in the art are familiar with, needs just carry out some routine tests.According to following example, more fully will understand the present invention, the use that these examples only lay down a definition, and in no case should be considered as limiting the present invention.

Example

Example is below for explaining the enforcement of various embodiments of the present invention.They are not intended to restriction or define four corner of the present invention.

the angiogenesis potentiality of the optical processing of the example 1 – embodiment of the present invention

In order to the angiogenesis potentiality of the light launched apparatus of the present invention, system and method are evaluated, establish human body skin model.Briefly, adopt the system irradiation of one embodiment of the invention containing fibroblast and Keratinocytic human body skin model 5 minutes, the light that described system provides has the characteristic shown in Fig. 7 a and 7b, adopt spectroradiometer (SP-100 of ORBOptronix) to measure, indicate the power density in the optical processing time of cell reception in this example skin model.

Particularly, the light that skin model receives comprises wave-length coverage about 400 to about 470nm, the first peak of the about 450nm of peak wavelength, and the about 520nm of wave-length coverage to about 620nm, second peak of the about 540-580nm of peak wavelength.At process distance (apart from skin model 10cm), the peak power at first peak peak wavelength place is about 1.5-2mW/cm ²/ nm, the peak power at the second peak-to-peak value wavelength place is about 0.01mW/cm ²/ nm (being about 0.5% of first peak).During process in 5 minutes, the general power of first peak is about 35-55mW/cm ²(about 38-45mW/cm ²).The general power at the second peak is about 0.05-0.6mW/cm ²(about 0.4-0.5mW/cm ²), be no more than about 0.1-1.5% (about 1%) of first peak general power.The bandwidth at the second peak is about 20-60nm, approximately 40-60nm, wider than the bandwidth (about 20nm) of first peak.Within 5 minute processing time, the energy density that skin model receives is about 10-20J/cm ², about 15J/cm ².In 5 minute processing time, the second main peak is about 0.3-1% (about 0.6%) of the total energy density of the light that skin model receives.The power density of light that skin model receives compare (ratio of activation light and fluorescence) change during processing.

The system providing illumination to penetrate skin model comprises the lamp of transmitting " activation " blue light (similar shown in emission characteristics and Fig. 3) and the compositions containing fluorescent agent (such as eosin), and compositions is activated by lamp, launches fluorescence.Particularly, lamp has two groups of LED of peak wavelength 410-420nm and 440-470nm.At distance LED10cm place, the peak wavelength of activation light is about 450nm, and the power density at peak wavelength place is about 2-3mW/cm ²/ nm (about 2.5mW/cm ²/ nm), mean power is about 55-65mW/cm ², within 5 minutes, irradiation time self-energy density is about 15-25J/cm ²(about 16-20J/cm ²).

The nylon wire in 20 microns, aperture is utilized skin model and bio-photon compositions to be separated.Skin model irradiates with activation light and fluorescence substantially simultaneously.

Because the contact of bio-photon compositions and cell is limited, fibroblast and keratinocyte are mainly exposed to the fluorescence that activation light and bio-photon compositions are launched.Then, to plating before at Matrigel ^tMin the upper and ill microvascular endothelial cells of the human aortic endothelial cells of diabetics on apply conditioned medium from treated human body 3D skin model.By microscopic examination and the formational situation of monitoring endotheliocyte pipe after 24 hours.The conditioned medium of the 3D skin model of photo-irradiation treatment of hanging oneself induces leather hose formation in vitro in two kinds of cell types, show that optical processing (having the light that peak wavelength is in two peaks in blue and green spectral range) is by producing cytokine by fibroblast and keratinocyte, and to vascularization, there are remote-effects.Adopt from the Nostoc commune Vanch liquid of undressed skin samples and conditioned medium in contrast, they all do not have the formation of inducing endothelial pipe.

example 2-protein secreting and gene expression profile

Adopt the injured potentiality triggering obvious protein secreting and gene expression profile with the light that unscathed 3D human body skin model (EpiDermFT, MatTekCorporation) coming evaluates apparatus of the present invention, system and method is launched.Briefly, will (adopt somatomedin, 50% somatomedin and not adopt somatomedin) similar illumination shown in injured and unscathed 3D human body skin model characteristic and Fig. 7 a and 7b that cultivate to penetrate 2 minutes under different conditions.Irradiate the system of skin model comprise transmittings " activation " blue light lamp and contain two kinds of fluorescent agents (eosin and erythrosine), can when being activated by lamp the compositions of emitting fluorescence.

The nylon wire in 20 microns, aperture is utilized skin model and compositions to be separated, the about 10cm of lamp distance skin model.Skin model irradiates with activation light and fluorescence substantially simultaneously.Control sample forms by not adopting light-struck 3D skin model.

Gene expression profile and protein secreting spectrum are measured in illumination after penetrating 24 hours.By the secretion of antibody array (RayBioHumanCytokine antibody array) the analysis of cells factor, by PCR array (PAHS-013A, SABioscience) analyzing gene is expressed, and measures cytotoxicity by GAPDH and LDH release.Result (table 1 and table 2) shows, on compromised skin Insert Fragment, in non-starvation, optical processing can improve protein secreting and the gene expression dose in wound healing Preliminary Irritation stage.Interestingly, on a cellular level, optical processing far below its impact on compromised skin Insert Fragment, shows that optical processing has the impact on cellular level on the impact of non-compromised skin model.Seem to regulate the amboceptor relevant with inflammation.Owing to lacking other cell type, such as, macrophage in 3D skin model, does not occur that antiinflammatory feeds back, and this seems to may be used for the reason explaining that wound closure postpones.Cytotoxicity is not observed in optical processing.

Table 1 – is the 3rd day time, and the secretion between treated sample and unprocessed control sample is than the inventory of protein with statistically-significant difference.Double-head arrow represents that secretion ratio is more than 2 times.

24 hours periods of table 2 – first treated sample and the expression ratio of unprocessed control sample there is the inventory of the gene of statistically-significant difference.Double-head arrow represents that secretion ratio is more than 2 times.

example 3 – in time and distance change power density

Fig. 8 and Fig. 9 indicates how according to the embodiment of the present invention, is changed general power or the energy density of emission peak by the distance changed between light source and treatment sites, selects suitable medical science or beauty therapeutic optical processing or system schema.In addition, if there are two light sources with two peaks, the relative power of each light source can change in time, and Fig. 8 indicates the decline situation of general power within the processing time of 5 minutes.Fig. 9 indicates general power to be increased in time.In one embodiment, the system that Fig. 8 and Fig. 9 can comprise emission characteristics lamp as shown in Figure 3 and a kind of fluorescent composition by one produced, Fig. 8 shows that the general power of the fluorescence that compositions is launched declines in time, and Fig. 9 shows the increase of the power of the lamp transmitted by bio-photon compositions.

example 3-collagen protein is formed

Use up and irradiate corium human fibroblasts, and in the formation of collagen protein, it is evaluated.By corium human fibroblasts plating at the bottom of glass with holes culture dish ( ) in.About 4000 cells are had in every hole.After 48 hours, culture dish at the bottom of glass is turned around, following process is carried out to the cell of glass bottom: irradiation that (A) is unglazed (contrast), (B) about 13 minutes of sun exposure (contrast) is utilized at noon, (C) add the about 550-570nm of peak wavelength (the about 480-620nm of wave-length coverage) with daylight, peak wavelength place peak power about 0.01 is to about 0.065mW/cm ²/ nm, the about 3-5mW/cm of total peak power ², the light of the about 50-70nm of bandwidth irradiates, and (D) irradiates with the light comprising two kinds of emission peaks, and the peak wavelength at first peak is about 440-460nm, the about 18-23nm of bandwidth, the about 25-95mW/cm of total peak power ², peak wavelength place peak power about 1 is to about 3.5mW/cm ²/ nm, the about 550-570nm of peak wavelength at second peak, the about 50-70nm of bandwidth, the about 3-5mW/cm of total peak power ², peak wavelength place peak power about 0.01 is to about 0.065mW/cm ²/ nm (as as-shown-in figures 10 a and 10b).

After treatment, washed cell, and cultivate 48 hours in Nostoc commune Vanch liquid.Then, adopt picrosirius-red method, collagen assay is carried out to the supernatant.This comprises and is added in supernatant by the picric acid solution of Picro-Sirius red dyestuff, shaken cultivation 30 minutes gently, then centrifugalize, forms granule.First use 0.1NHCl, then use 0.5NNaOH washing granule, remove free dye.After centrifugalize, concerning I-type collagen, read the reading of suspension at 540nm place.The results are shown in Table 3.

The concentration of I-type collagen in the corium human fibroblasts supernatant be exposed in above-mentioned A, B, C and D processing method has been carried out qualitative contrast by table 3 –.++ represent collagen content be+twice, +++ represent collagen content be+three times.

The collagen content irradiating (C) light and (D) light compares existence marked difference statistically with unglazed with only sun-exposed control sample.

The generation of collagen protein indicates the potentiality of tissue repair, comprises stablizing of granulation tissue and diminishing of wound size.Also reduce with microgroove, pore shrinks, the improved texture of intact skin and the improvement of hot strength be associated.

Can rational expectation, adopt light emission characteristic and system class described in example 1-3 seemingly or suitable system or device, same or similar effect can be provided.Such as, system can comprise a lamp and a kind of luminophor.

example 4 – antibacterial effect

The bacterial suspension of propionibacterium acnes (p.acnes) is placed in 12 orifice plates, in anaerobic room, cultivates about 24h at about 35 DEG C.Following light irradiation inoculum by the embodiment of the present invention: (A) first lamp, launches the unimodal of about 440-470nm, the about 18-22nm of bandwidth, apart from the about 60-150mW/cm of suspension 5cm place general power ², after irradiating about 5 minutes, the total energy density irradiating suspension is about 18-39J/cm ².The about 400-500nm of total emission wavelength.There is no ultraviolet light; And (B) second lamp, emission characteristics and Fig. 3 similar, launching the peak wavelength of first peak is about 440-470nm, and bandwidth is about 18-22nm, and peak wavelength about 410-420nm, the 5cm place general power at the second peak is about 60-150mW/cm ²(about 125mW/cm ²), after 5 minutes, total energy density is about 18-39J/cm ²(about 35J/cm ²).The about 400-500nm of total emission wavelength.Do not launch any ultraviolet light.

Be no more than the optical density of the bacterial suspension of 2 hours by measurement pre-irradiation and irradiation, bacterial growth level is evaluated.By result with contrast (do not carry out light-struck bacterial suspension, and not containing the medium of antibacterial) and compare.Result is as shown in Figure 11 a and Figure 11 b.Figure 11 a shows, compared with the bacterial growth situation not connecing irradiated reference substance, irradiates 5 minutes, have impact after irradiation at least 2 hours on the growth of propionibacterium acnes with lamp (A).Figure 11 b shows, compared with the bacterial growth situation not connecing irradiated reference substance, irradiates 5 minutes, have impact after irradiation at least 2 hours on the growth of propionibacterium acnes with lamp (B).Observe in addition, when adopting lamp A and lamp B to irradiate, illumination approximately can continue 20-25 hour after irradiation on the impact of the activity of propionibacterium acnes.These results show, after irradiating, have bacteriostasis at least in a short time to propionibacterium acnes with lamp A and lamp B, within the longer time, have bactericidal action to propionibacterium acnes.

Also demonstrate lamp A and lamp B can affect the growth of other Gram-positive and gram negative strain (such as at least in a short time, antibacterial), include but not limited to staphylococcus aureus, methicillin-resistant staphylococcus aureus, clostridium difficile, Klebsiella Pneumoniae and Pseudomonas aeruginosa.These lamps also affect some bacterial strains in (such as bactericidal effect) these bacterial strains and other bacterial strain within the longer time, the activity of such as staphylococcus aureus and staphylococcus epidermidis.

These lamps are used together with the bio-photon compositions containing fluorescent dye, shows, to bacterial strains such as propionibacterium acness, there is antibacterial and sterilization complementary effect.

Will be appreciated that aforementioned explanation is only exemplary, be not limited to various details described herein.Although there is provided herein several embodiments of the present invention, will be appreciated that system disclosed herein, apparatus and method and parts thereof, can implement with other many concrete form, only otherwise deviate from scope of the present invention.

To those skilled in the art, various change and transformation can be made to the present invention after reading the present invention.Feature disclosed herein can be implemented with any combination and point combination (comprising the combination of several dependency and point combination) with other features one or more described herein.Various feature recited above, comprises their any parts, can combine or be integrated in other system.In addition, some feature can be omitted or not implement.

Those of ordinary skill in the art can change, replace and change these examples, only otherwise deviate from scope of the present invention.The equal by reference entirety of all lists of references cited herein is incorporated in the application, and forms a application's part.

Claims

1. a phototherapy device, comprising:

First light source, described first light source can launch the first light with the first peak wavelength, for activate be applied in above treatment sites or neighbouring can photoactivatable composition; And

Secondary light source, described secondary light source can launch the second light with the second peak wavelength, and the second peak wavelength is different from the first peak wavelength, and wherein the first peak wavelength and the second peak wavelength are in blue light and/or the purple light district of electromagnetic spectrum.

2. a phototherapy device, comprising:

First light source, described first light source can launch the first light with the first peak wavelength;

Secondary light source, described secondary light source can launch the second light with the second peak wavelength, and the second peak wavelength is different from the first peak wavelength, and wherein the first peak wavelength is in blue light and/or the purple light district of electromagnetic spectrum.

3. device according to claim 1 and 2, wherein the first peak wavelength be about 430 to about 500nm, about 440 to about 500nm, about 450 to about 500nm, about 430 to about 475nm, approximately 435nm to about 470nm, approximately 440nm to about 460nm, approximately 445nm to about 455nm, approximately 440nm, approximately 450nm, approximately 460nm or about 470nm.

4. the device according to claim 1 or any one of claim 3, wherein the second peak wavelength is about 400nm to about 500nm, approximately 400nm to about 475nm, approximately 400nm to about 450nm, approximately 400nm to about 430nm or approximately 410nm to about 420nm or approximately 415nm.

5. device according to claim 1, wherein the second peak wavelength is about 410nm to about 430nm, and the peak wavelength of the first light is about 440nm to about 470nm.

6. according to claim 2 or device according to claim 3, wherein the second peak wavelength be about 480 to about 760nm, approximately 480nm to about 700nm, approximately 480nm to about 650nm, approximately 480nm to about 620nm, about 500 to about 700nm, approximately 520nm to about 700nm, approximately 500nm to about 660nm, approximately 540nm to about 640nm, approximately 540nm to about 580nm, approximately 500nm to about 570nm, approximately 570nm to about 590nm, approximately 590nm to about 610nm, or about 610nm to about 760nm.

7., according to claim 2 or device according to claim 3, wherein the second peak wavelength is in the infrared region of electromagnetic spectrum.

8. the device according to any one of claim 1 to 7, wherein in the first light and the second light, the bandwidth of at least one light is equal to or less than about 20nm.

9. the device according to any one of claim 1 to 7, wherein in the first light and the second light, the bandwidth of at least one light is about 19nm ± 5nm.

10. the device according to any one of claim 1 to 7, wherein the bandwidth of the second light is about 15nm extremely about 100nm, approximately 25nm to about 80nm, approximately 30nm to about 70nm, or about 20nm to about 50nm.

11. devices according to any one of claim 1 to 10, wherein the band of the first light of bandwidth ratio of the second light is wide.

12. devices according to claim 11, wherein the bandwidth of the first light is about 15nm to about 25nm, and the bandwidth of the second light is about 20nm to about 100nm.

13. devices according to any one of claim 1 to 12, that wherein said device is launched and/or treatment sites is about 4 to about 75mW/cm from the average power density that described device receives ², about 15 to about 75mW/cm ², about 10 to about 200mW/cm ², about 10 to about 150mW/cm ², about 20 to about 130mW/cm ², about 55 to about 130mW/cm ², about 90 to about 140mW/cm ², about 100 to about 140mW/cm ², or about 110 to about 135mW/cm ².

14. devices according to any one of claim 1 to 12, that wherein said device is launched and/or treatment sites is about 4 to about 75mW/cm from the average power density that described device receives ², about 15 to about 75mW/cm ², about 10 to about 85mW/cm ², about 10 to about 75mW/cm ², about 30 to about 70mW/cm ², about 40mW/cm ²to about 70mW/cm ², about 55 to about 65mW/cm ², or about 55 to about 85mW/cm ².

15. devices according to any one of claim 1 to 14, wherein the average power density of the second light is lower than the average power density of the first light.

16. devices according to claim 15, wherein the average power density of the second light is about 0.1% of the first light to about 90%, about 0.1% to about 80%, about 0.1% to about 70%, about 0.1% to about 60%, about 0.1% to about 50%, about 0.1% to about 40%, about 0.1% to about 30%, about 0.1% to about 20%, about 0.1% to about 10%, about 0.1% to about 9%, about 0.1% to about 8%, about 0.1% to about 7%, about 0.1% to about 6%, about 0.1% to about 5%, about 0.1% to about 4%, about 0.1% to about 3%, about 0.1% to about 2%, about 0.1% to about 1%.

17. devices according to any one of claim 1 to 16, wherein device described in single treatment launch and/or the energy density of light that treatment sites receives from described device be greater than about 4J/cm ², be greater than about 10J/cm ², be greater than about 15J/cm ², be greater than about 30J/cm ², be greater than about 50J/cm ², be no more than about 60J/cm ².

18. devices according to any one of claim 1 to 16, wherein device described in single treatment launch and/or the energy density of light that treatment sites receives from described device be about 4J/cm ²to about 60J/cm ², about 10J/cm ²to about 60J/cm ², about 10J/cm ²to about 50J/cm ², about 10J/cm ²to about 40J/cm ², about 10J/cm ²to about 30J/cm ², about 20J/cm ²to about 40J/cm ², about 15J/cm ²to about 25J/cm ², or about 10J/cm ²to about 20J/cm ².

19. devices according to any one of claim 1 to 18, wherein secondary light source is launched or the energy density that is transferred to treatment sites is launched lower than the first light source or is transferred to the energy density for the treatment of sites.

20. devices according to claim 19, wherein process at every turn secondary light source launch or the energy density that is transferred to treatment sites be the first light source launch or be transferred to treatment sites energy density about 0.1% to about 90%, about 0.1% to about 80%, about 0.1% to about 70%, about 0.1% to about 60%, about 0.1% to about 50%, about 0.1% to about 40%, about 0.1% to about 30%, about 0.1% to about 20%, about 0.1% to about 10%, about 0.1% to about 9%, about 0.1% to about 8%, about 0.1% to about 7%, about 0.1% to about 6%, about 0.1% to about 5%, about 0.1% to about 4%, about 0.1% to about 3%, about 0.1% to about 2%, about 0.1% to about 1%.

21. devices according to any one of claim 1 to 7, wherein the first light of bandwidth ratio of the second light is wide, and average power density is lower than the first light.

22. devices according to claim 21, wherein the energy density of the second light is lower than the first light.

23. devices according to any one of claim 1 to 22, wherein the peak power of the second light at the second peak wavelength place is lower than the peak power of the first light at the first peak wavelength place.

24. devices according to any one of claim 1 to 23, comprise the controller with the first light source and secondary light source electronic communication further, for the second emission spectra of the first emission spectra and/or the second light that change the first light, wherein the first emission spectra and the second emission spectra comprise the one or more emission spectra parameters being selected from bandwidth, peak wavelength, power density, launch time and energy density.

25. devices according to claim 24, wherein said controller can control separately one or more emission spectra parameters of often kind of light in the first light and the second light.

26. devices according to claim 24 or 25, wherein said controller regulates one or more emission spectra parameters of the first light and the second light according to the processing time.

27. devices according to any one of claim 1 to 26, comprise the 3rd light source further, wherein the 3rd light source can the about 500nm of emission peak wavelength to about 750nm, about 630 to about 750nm, or the third light of about 750nm to about 1mm.

28. devices according to any one of claim 1 to 27, wherein the first light source and secondary light source can launch incoherent light.

29. devices according to any one of claim 1 to 28, wherein first and/or secondary light source comprise one or more light emitting diode (LED).

30. devices according to any one of claim 1 to 29, wherein the first light source and secondary light source by arranged in arrays at least one block of plate.

31. devices according to claim 30, wherein the first light source and secondary light source are spaced about 4 to 7mm, about 4 to 10mm, about 10 to 15mm, or about 15 to 20mm.

32. devices according to claim 30 or 31, comprise the plate that polylith can connect, these plates can move relative to each other.

33. devices according to claim 32, comprise the plate controller with these plate electronic communications, the independent light emission controlling these plates.

34. devices according to any one of claim 30 to 33, wherein secondary light source is dispersed in the first light source in an array.

35. devices according to any one of claims 1 to 34, wherein the first light source and secondary light source equidistant placement substantially.

36. devices according to any one of claims 1 to 35, comprise the one or more wave filter filtering the first light and/or the second light further.

37. devices according to any one of claims 1 to 36, comprise the collimator for calibrating the first light and/or the second light further.

38. devices according to any one of claims 1 to 37, wherein the first light source and secondary light source are positioned on the surface of emission of described device, and described device comprises further and stretches out from the surface of emission spacing probe being used to indicate process distance.

39. devices according to any one of claims 1 to 38, comprise at least one air vent for cooling described device further at the shell of carrying first and/or secondary light source.

40. 1 kinds of phototherapy devices, comprising:

First light source, described first light source can about 400 to the 750nm of emission peak wavelength, and average power density about 4 is to about 75mW/cm ², or about 55mW/cm ²to about 150mW/cm ²the first light.

41. 1 kinds of phototherapy devices, comprising:

First light source, described first light source can emission peak wavelength about 400 to the first light of the 5nm of about 750nm, the about 19nm of bandwidth ± approximately.

42. 1 kinds of phototherapy devices, comprising:

Comprise and launch the first light source of the first light, the peak wavelength of the first light is about 400 to about 750nm, and bandwidth is about 15nm to about 100nm, approximately 25nm to about 80nm, approximately 30nm to about 70nm, or about 20nm to about 50nm.

43. 1 kinds of phototherapy devices, comprising:

Launch the first light source of the first light, the peak wavelength of the first light is about 400 to about 750nm, and single treatment energy density is about 4 to about 60J/cm ², about 10 to about 60J/cm ², about 10 to about 50J/cm ², about 10 to about 40J/cm ², about 10 to about 30J/cm ², about 20 to about 40J/cm ², about 15J/cm ²to 25J/cm ², or about 10 to about 20J/cm ².

44. devices according to any one of claim 41 to 44, wherein the average power density of the first light is about 4 to about 75mW/cm ², or about 55mW/cm ²to about 150mW/cm ².

45. devices according to any one of claim 40 to 42, wherein the energy density of the first light single treatment is about 4 to about 60J/cm ², about 10 to about 60J/cm ², about 10 to about 50J/cm ², about 10 to about 40J/cm ², about 10 to about 30J/cm ², about 20 to about 40J/cm ², about 15J/cm ²to 25J/cm ², or about 10 to about 20J/cm ².

46. devices according to any one of claim 40,41 or 43, wherein the bandwidth of the first light be about 400 to about 750nm, the about 15nm of bandwidth to about 100nm, approximately 25nm to about 80nm, approximately 30nm to about 70nm, or about 20nm to about 50nm.

47. devices according to any one of claim 40,42 or 43, wherein the bandwidth of the first light is about 19nm ± approximately 5nm.

48. devices according to any one of claim 40 to 47, wherein the first light source comprises light emitting diode matrix (LED).

49. the device according to any one of claim 40 to 48, wherein said peak wavelength is about 400nm to about 450nm, approximately 450nm to about 490nm, approximately 490nm to about 560nm, approximately 560nm to about 590nm or approximately 590nm to about 635nm or approximately 635nm to about 720nm.

50. devices according to any one of claim 40 to 50, comprise controller further, for changing the power density of the first light, bandwidth, peak wavelength, time or energy density during the first light emission.

51. devices according to claim 50, wherein said controller can reduce the power density of the first light during the first light emission of device.

52. devices according to any one of claim 48 to 51, wherein the first light source and secondary light source are spaced about 4 to 7mm, about 4 to 10mm, about 10 to 15mm, or about 15 to 20mm.

53. devices according to claim 48 or 52, wherein LED array is placed on above plate, and described device comprises the plate of one or more removable connection each other.

54. devices according to claim 53, comprise the plate controller with one or more plate electronic communication described, control one or more plate described for independent.

55. devices according to any one of claim 40 to 54, comprise the one or more wave filter filtering the first light further.

56. devices according to any one of claim 40 to 55, comprise the collimator for calibrating the first light and/or the second light further.

57. devices according to any one of claim 40 to 56, wherein the first light source is positioned on the surface of emission of described device, and described device comprises spacing probe that stretch out from the surface of emission, that be used to indicate process distance further.

58. devices according to any one of claim 40 to 57, comprise at least one air vent for cooling further at the shell of carrying first light source.

59. devices according to any one of claim 40 to 58, comprise the secondary light source launched the second light, have peak wavelength further, wherein the second light has at least one feature in following characteristics:

F) band is wider than the bandwidth of the first light,

G) average power density is lower than the average power density of the first light,

H) power at peak wavelength place is lower than the power at the first peak wavelength place,

I) peak wavelength is longer than the peak wavelength of the first light, or

J) during process, energy density is lower than the first light.

60. devices according to claim 59, wherein the average power density of the second light is about 0.1% of the first light to about 90%, about 0.1% to about 80%, about 0.1% to about 70%, about 0.1% to about 60%, about 0.1% to about 50%, about 0.1% to about 40%, about 0.1% to about 30%, about 0.1% to about 20%, about 0.1% to about 10%, about 0.1% to about 9%, about 0.1% to about 8%, about 0.1% to about 7%, about 0.1% to about 6%, about 0.1% to about 5%, about 0.1% to about 4%, about 0.1% to about 3%, about 0.1% to about 2%, about 0.1% to about 1%.

61. devices according to claim 59, wherein process at every turn secondary light source launch or the energy density that is transferred to treatment sites be the first light source launch or be transferred to treatment sites energy density about 0.1% to about 90%, about 0.1% to about 80%, about 0.1% to about 70%, about 0.1% to about 60%, about 0.1% to about 50%, about 0.1% to about 40%, about 0.1% to about 30%, about 0.1% to about 20%, about 0.1% to about 10%, about 0.1% to about 9%, about 0.1% to about 8%, about 0.1% to about 7%, about 0.1% to about 6%, about 0.1% to about 5%, about 0.1% to about 4%, about 0.1% to about 3%, about 0.1% to about 2%, about 0.1% to about 1%.

62. devices according to claim 59, wherein the band of the second light is wider than 20nm, approximately 20-100nm, approximately 20-80nm, approximately 20-60nm, approximately 20-40nm.

63. devices according to claim 59, wherein the power at the second peak wavelength place is about 0.1% of the first light to about 90%, about 0.1% to about 80%, about 0.1% to about 70%, about 0.1% to about 60%, about 0.1% to about 50%, about 0.1% to about 40%, about 0.1% to about 30%, about 0.1% to about 20%, about 0.1% to about 10%, about 0.1% to about 9%, about 0.1% to about 8%, about 0.1% to about 7%, about 0.1% to about 6%, about 0.1% to about 5%, about 0.1% to about 4%, about 0.1% to about 3%, about 0.1% to about 2%, about 0.1% to about 1%.

64. 1 lamps, comprise

A lamp holder, has multiple light emitting diode by arranged in arrays, and described array comprises at least the first group LED and second group LED, and the often group LED wherein in first group of LED and second group LED comprises at least one LED;

Lamp controller, is connected with lamp holder, has the circuit controlling and operate LED;

Wherein first group of LED can produce the first incoherent light of the about 430nm of peak wavelength to about 500nm;

Wherein second group of LED can produce the second incoherent light of the about 400nm of peak wavelength to about 430nm;

Wherein lamp holder can produce or the power density of light that treatment surface can receive be about 4 to about 75mW/cm ², or about 55mW/cm ²to about 150mW/cm ².

65. lamps according to claim 64, wherein first group of LED can the roomy about 19nm ± 5nm of generating strap, or the light of about 13 to about 26nm.

66. lamps according to claim 64 or 65, wherein second group of LED can the light of the roomy about 13nm of generating strap to about 20nm.

67. lamps according to any one of claim 64 to 66, comprise the 3rd group of LED further, and wherein the 3rd group of LED produces the incoherent light of the about 500nm to 750nm of peak wavelength.

68. lamps according to any one of claim 64 to 67, can arrange controller, with the one or more parameters allowing it change first and/or the second light, this one or more parameter is selected from power density, bandwidth, wavelength, integral energy and launch time.

69. lamps according to any one of claim 64 to 68, wherein in array, first group of LED and second group LED is spaced about 4 to 7mm, about 4 to 10mm, about 10 to 15mm, or about 15 to 20mm.

70. lamps according to any one of claim 64 to 69, wherein first group of LED and second group LED dispersed placement each other.

71. lamps according to any one of claim 64 to 70, wherein first group of LED and second group LED equidistant placement substantially in an array.

72. lamps according to any one of claim 64 to 71, wherein said lamp holder comprises the plate that polylith places LED, and described plate can move relative to each other.

73. according to the lamp described in claim 72, and wherein said lamp controller can control separately the LED of every block plate.

74. lamps according to any one of claim 64 to 73, comprise the one or more wave filter filtering the first light and/or the second light further.

75. lamps according to any one of claim 64 to 74, comprise the collimator for calibrating the first light and/or the second light further.

76. lamps according to any one of claim 64 to 75, wherein first group of LED and second group LED array is positioned on the surface of emission of described lamp, and described lamp comprises further and stretches out from the surface of emission spacing probe being used to indicate process distance.

77. lamps according to any one of claim 64 to 76, comprise at least one air vent for cooling further at lamp holder.

78. lamps according to any one of claim 64 to 77, wherein during single treatment, lamp can be launched or to treatment surface transmission about 4 to about 60J/cm ², about 10 to about 60J/cm ², about 10 to about 50J/cm ², about 10 to about 40J/cm ², about 10 to about 30J/cm ², about 20 to about 40J/cm ², about 15J/cm ²to 25J/cm ², or about 10 to about 20J/cm ²energy density.

79. lamps according to any one of claim 64 to 78, wherein said power density is about 10 to about 75mW/cm ², about 15 to about 75mW/cm ², about 10 to about 150mW/cm ², about 20 to about 130mW/cm ², about 55 to about 130mW/cm ², about 90 to about 140mW/cm ², about 100 to about 140mW/cm ², about 110 to about 135mW/cm ², about 10 to about 85mW/cm ², about 30 to about 70mW/cm ², about 40 to about 70mW/cm ², about 55 to about 65mW/cm ², or about 55 to about 85mW/cm ².

80. 1 lamps, comprise a lamp holder, have multiple light emitting diode by arranged in arrays, and described array comprises at least the first group LED and second group LED, and the often group LED wherein in first group of LED and second group LED comprises at least one LED;

Lamp controller, is connected with lamp holder, has the circuit controlling and operate LED; Wherein first group of LED can produce peak value

The incoherent light of the about 400nm of wavelength to about 500nm; Wherein second group of LED can produce the light of peak wavelength about 500 to about 760nm;

The power density of the light that wherein said lamp can produce is 4 to about 75mW/cm ², or about 55 to about 150mW/cm ².

81. lamps according to Claim 8 described in 0, wherein first group of LED can the light of the roomy about 17nm of generating strap to about 23nm.

82. lamps according to Claim 8 described in 0 or 81, wherein second group of LED can the roomy about 20nm of generating strap to about 100nm, approximately 25nm to about 80nm, approximately 30nm to about 70nm, or the light of about 20nm to about 60nm.

83. lamps according to Claim 8 described in 0 to 82 any one, wherein power density ratio second group of LED of light of producing of first group of LED is high.

84. lamps according to Claim 8 described in 0 to 83 any one, wherein said controller is for changing one or more parameters of first and/or the second light, and one or more parameter is selected from power density, bandwidth, wavelength, integral energy and launch time.

85. lamps according to Claim 8 described in 0 to 84 any one, wherein in array, first group of LED and second group LED is spaced about 4 to 7mm, about 4 to 10mm, about 10 to 15mm, or about 15 to 20mm.

86. lamps according to Claim 8 described in 0 to 85 any one, wherein first group of LED and second group LED dispersed placement each other.

87. lamps according to Claim 8 described in 0 to 86 any one, wherein first group of LED and second group LED equidistant placement substantially in an array.

88. lamps according to Claim 8 described in 0 to 87 any one, wherein said lamp holder comprises the plate that polylith places LED, and described plate can move relative to each other.

89. lamps according to Claim 8 described in 8, wherein said lamp controller can control separately the LED of every block plate.

90. lamps according to Claim 8 described in 0 to 89 any one, comprise the one or more wave filter filtering the first light and/or the second light further.

91. lamps according to Claim 8 described in 0 to 90 any one, comprise the collimator for calibrating the first light and/or the second light further.

92. lamps according to Claim 8 described in 0 to 91 any one, wherein first group of LED and second group LED array is positioned on the surface of emission of described lamp, and described lamp comprises spacing probe that stretch out from the surface of emission, that be used to indicate process distance further.

93. lamps according to Claim 8 described in 0 to 92 any one, comprise at least one air vent for cooling further at lamp holder.

94. lamps according to Claim 8 described in 0 to 93 any one, wherein during single treatment, lamp can be launched or to treatment surface transmission about 4 to about 60J/cm ², about 10 to about 60J/cm ², about 10 to about 50J/cm ², about 10 to about 40J/cm ², about 10 to about 30J/cm ², about 20 to about 40J/cm ², about 15J/cm ²to 25J/cm ², or about 10 to about 20J/cm ²energy density.

95. lamps according to Claim 8 described in 0 to 94 any one, wherein said power density is about 10 to about 75mW/cm ², about 15 to about 75mW/cm ², about 10 to about 150mW/cm ², about 20 to about 130mW/cm ², about 55 to about 130mW/cm ², about 90 to about 140mW/cm ², about 100 to about 140mW/cm ², about 110 to about 135mW/cm ², about 10 to about 85mW/cm ², about 30 to about 70mW/cm ², about 40 to about 70mW/cm ², about 55 to about 65mW/cm ², or about 55 to about 85mW/cm ².

96., by lamp described in device described in any one of claim 1 to 63 or any one of claim 64 to 95, can use together with photoactivatable composition with one.

97. according to the purposes described in claim 96, wherein saidly can comprise at least one light activating agent by photoactivatable composition, the peak excitation wavelength of described light activating agent is about 400 to about 700nm, about 420 to about 540nm, about 420 to about 590nm, or about 450nm to about 700nm.

98. purposes according to claim 96 or 97, wherein said light activating agent is fluorescent agent.

Lamp described in device described in 99. any one of claim 1 to 63 or any one of claim 64 to 95, the purposes in the cosmetic treatments of tissue.

100. according to the purposes described in claim 99, and wherein said cosmetic treatments is selected from skin renewal, skin condition, reduces pore, increases tissue intensity, reduces or eliminates and organize cicatrix.

Lamp described in device described in 101. any one of claim 1 to 63 or any one of claim 64 to 95, the purposes in treating skin disease.

102. according to the purposes described in claim 101, and wherein said dermatosis comprises acne, psoriasis, eczema, dermatitis, inflammation and inflammatory dermatosis.

Lamp described in device described in 103. any one of claim 1 to 63 or any one of claim 64 to 95, the purposes in wound healing.

Lamp described in device described in 104. any one of claim 1 to 63 or any one of claim 64 to 95, the purposes in treatment antibacterial, virus or fungal infection.

Lamp described in device described in 105. any one of claim 1 to 63 or any one of claim 64 to 95, in the purposes providing antibacterial and/or in bactericidal action.

106. mobile devices activation can the purposes of photoactivatable composition, wherein mobile device can launch emission spectra with can the absorption spectrum of light activating agent is overlapping in photoactivatable composition light.

107. television sets or computer display activation can the purposes of photoactivatable composition, wherein the emission spectra of light that sends of display screen with can in photoactivatable composition the absorption spectrum of light activating agent overlapping.

108. purposes according to claim 106 or 107, wherein said mobile device or display screen have light source, and described light source can after about 15 minutes, about 30 minutes, about 45 minutes, 1 hour, 2 hours, 3 hours, 4 hours, 5 hours, 6 hours, 7 hours or 8 hours, photoactivation can photoactivatable composition in irradiation.

109. purposes according to any one of claim 106 to 108, wherein saidly can comprise at least one light activating agent by photoactivatable composition, the peak excitation wavelength of described light activating agent is about 400 to about 700nm, about 420 to about 540nm, approximately 540nm to about 590nm, about 590 to about 635nm, or about 635 to about 700nm.

110. purposes according to any one of claim 106 to 108, wherein said mobile device, television set or computer display comprise the device described in any one of claim 1 to 63; Or the lamp described in any one of claim 64 to 95.

Organize beauty treatment or the method for Medical Treatment for 111. one kinds, described method comprises:

Adopt have can to can the first illumination of the first peak wavelength of activating of photoactivatable composition penetrate be applied in above organized processing position or neighbouring can photoactivatable composition or irradiate structural treatment sites;

Adopt the second illumination penetrate identical or different on organized processing position can photoactivatable composition or irradiate the treatment sites of described tissue, the second light has the second peak wavelength, second peak wavelength is different from the first peak wavelength, and wherein the first peak wavelength and the second peak wavelength are all in blue light and/or the purple light district of electromagnetic spectrum.

Organize beauty treatment or the method for Medical Treatment for 112. one kinds, described method comprises:

The first illumination with the first peak wavelength is adopted to penetrate described tissue;

Adopt the second illumination with the second peak wavelength to penetrate described tissue, the second peak wavelength is different from the first peak wavelength, and wherein the first peak wavelength is in blue light and/or the purple light district of electromagnetic spectrum.

113. according to the method described in claim 112, is included in that use on organized processing position or near treatment sites before irradiating tissue can the compositions of photoactivation further.

114. methods according to any one of claim 111 to 113, wherein the first peak wavelength be about 430 to about 500nm, about 440 to about 500nm, about 450 to about 500nm, about 430 to about 475nm, approximately 440nm to about 460nm, approximately 445nm to the about 435nm of about 455nm to about 470nm, approximately 440nm, approximately 450nm, approximately 460nm or about 470nm.

115. the method according to claim 111 or any one of claim 113-114, wherein the peak wavelength of the second light is about 400nm to about 500nm, approximately 400nm extremely about 475nm, approximately 400nm to about 450nm, approximately 400nm to about 430nm or about 410nm to about 420nm, approximately 415nm.

116. according to the method described in claim 111, and wherein the first peak wavelength is about 410nm to about 430nm, and the second peak wavelength is about 440nm to about 470nm.

117. methods according to any one of claim 112 to 114, wherein the second peak wavelength be about 480 to about 760nm, approximately 480nm to about 700nm, approximately 480nm to about 650nm, approximately 480nm to about 620nm, about 500 to about 700nm, approximately 520nm to about 700nm, approximately 500nm to about 660nm, approximately 540nm to about 640nm, approximately 540nm to about 580nm, approximately 500nm to about 570nm, approximately 570nm to about 590nm, approximately 590nm to about 610nm, or about 610nm to about 760nm.

118. methods according to any one of claim 112 to 114, wherein the second peak wavelength is in the infrared region of electromagnetic spectrum.

119. methods according to any one of claim 111 to 118, wherein in the first light and the second light, the bandwidth of at least one light is equal to or less than about 20nm.

120. methods according to any one of claim 111 to 119, wherein in the first light and the second light, the bandwidth of at least one light is about 19nm ± 5nm.

121. methods according to any one of claim 111 to 118, wherein the bandwidth of the second light is about 15nm to about 100nm, approximately 25nm to about 80nm, approximately 30nm to about 70nm, or about 20nm to about 50nm.

122. methods according to any one of claim 111 to 121, wherein the band of the first light of bandwidth ratio of the second light is wide.

123. according to the method described in claim 122, and wherein the bandwidth of the first light is about 15nm to about 25nm, and the bandwidth of the second light is about 20nm to about 100nm.

124. methods according to any one of claim 111 to 123, the first light wherein said and the average power density that the second is photoemissive and/or treatment sites receives are about 4 to about 75mW/cm ², about 15 to about 75mW/cm ², about 10 to about 200mW/cm ², about 10 to about 150mW/cm ², about 20 to about 130mW/cm ², about 55 to about 130mW/cm ², about 90 to about 140mW/cm ², about 100 to about 140mW/cm ², or about 110 to about 135mW/cm ².

125. methods according to any one of claim 111 to 123, the first light wherein said and the average power density that the second is photoemissive and/or treatment sites receives are about 4 to about 75mW/cm ², about 15 to about 75mW/cm ², about 10 to about 85mW/cm ², about 10 to about 75mW/cm ², about 30 to about 70mW/cm ², about 40mW/cm ²to about 70mW/cm ², about 55 to about 65mW/cm ², or about 55 to about 85mW/cm ².

126. methods according to any one of claim 111 to 125, wherein the average power density of the second light is lower than the average power density of the first light.

127. according to the method described in claim 126, wherein the average power density of the second light is about 0.1% of the first light to about 90%, about 0.1% to about 80%, about 0.1% to about 70%, about 0.1% to about 60%, about 0.1% to about 50%, about 0.1% to about 40%, about 0.1% to about 30%, about 0.1% to about 20%, about 0.1% to about 10%, about 0.1% to about 9%, about 0.1% to about 8%, about 0.1% to about 7%, about 0.1% to about 6%, about 0.1% to about 5%, about 0.1% to about 4%, about 0.1% to about 3%, about 0.1% to about 2%, about 0.1% to about 1%.

128. methods according to any one of claim 111 to 126, wherein during single treatment, the energy density of the light that treatment sites receives can be greater than about 4J/cm ², be greater than about 10J/cm ², be greater than about 15J/cm ², be greater than about 30J/cm ², be greater than about 50J/cm ², be no more than 60J/cm ².

129. methods according to any one of claim 111 to 126, wherein during single treatment, the energy density of the light that treatment sites receives is about 4J/cm ²to about 60J/cm ², about 10J/cm ²to about 60J/cm ², about 10J/cm ²to about 50J/cm ², about 10J/cm ²to about 40J/cm ², about 10J/cm ²to about 30J/cm ², about 20J/cm ²to about 40J/cm ², about 15J/cm ²to about 25J/cm ², or about 10J/cm ²to about 20J/cm ².

130. methods according to any one of claim 128 or 129, wherein single treatment comprise irradiate tissue or light activatable compositions be no more than about 30 minutes, about 1 minute to about 30 minutes, about 1 minute to about 25 minutes, about 1 minute to about 20 minutes, about 1 minute to about 19 minutes, about 1 minute to about 18 minutes, about 1 minute to about 17 minutes, about 1 minute to about 16 minutes, about 1 minute to about 15 minutes, about 1 minute to about 14 minutes, about 1 minute to about 13 minutes, about 1 minute to about 12 minutes, about 1 minute to about 11 minutes, about 1 minute to about 10 minutes, about 1 minute to about 9 minutes, about 1 minute to about 8 minutes, about 1 minute to about 7 minutes, about 1 minute to about 6 minutes, about 1 minute to about 5 minutes, about 1 minute to about 4 minutes, about 1 minute to about 3 minutes.

131. methods according to any one of claim 111 to 130, the energy density that wherein secondary light source is transferred to treatment sites is launched lower than the first light source or is transferred to the energy density for the treatment of sites.

132. according to the method described in claim 131, wherein process at every turn energy density that secondary light source is transferred to treatment sites be the first light source launch or be transferred to treatment sites energy density about 0.1% to about 90%, about 0.1% to about 80%, about 0.1% to about 70%, about 0.1% to about 60%, about 0.1% to about 50%, about 0.1% to about 40%, about 0.1% to about 30%, about 0.1% to about 20%, about 0.1% to about 10%, about 0.1% to about 9%, about 0.1% to about 8%, about 0.1% to about 7%, about 0.1% to about 6%, about 0.1% to about 5%, about 0.1% to about 4%, about 0.1% to about 3%, about 0.1% to about 2%, about 0.1% to about 1%.

133. methods according to any one of claim 111 to 132, wherein the first light of bandwidth ratio of the second light is wide, and average power density is lower than the first light.

134. according to the method described in claim 133, and wherein the energy density of the second light is lower than the first light.

135. methods according to any one of claim 111 to 134, wherein the peak power of the second light at peak wavelength place is lower than the peak power of the first light at peak wavelength place.

136. methods according to any one of claim 111 to 135, change the emission spectra of the first light and/or the second light during being included in single treatment further, wherein the emission spectra of the first light and the second light comprises one or more emission spectra parameters being selected from bandwidth, peak wavelength, power density, launch time and energy density.

137. methods according to any one of claim 111 to 136, wherein the first light and/or the second are only produced by light emitting diode (LED).

Organize beauty treatment or the method for Medical Treatment for 138. one kinds, described method comprises and adopts peak wavelength about 400 to about 750nm, and power density about 4 is to about 75mW/cm ², or about 55mW/cm ²to about 150mW/cm ²the first illumination penetrate tissue.

Organize beauty treatment or the method for Medical Treatment for 139. one kinds, described method comprises and adopts peak wavelength about 400 to about 750nm, and tissue is penetrated in the first illumination of 5nm of the about 19nm of bandwidth ± approximately.

Organize beauty treatment or the method for Medical Treatment for 140. one kinds, described method comprises employing peak wavelength about 400 to about 750nm, the about 15nm of bandwidth is about 100nm, approximately 25nm to about 80nm, approximately 30nm to about 70nm extremely, or tissue is penetrated in the first illumination of about 20nm to about 50nm.

Organize beauty treatment or the method for Medical Treatment for 141. one kinds, described method comprises and adopts peak wavelength about 400 to the first illumination of about 750nm to penetrate tissue, regulates at least one parameter in the peak wavelength of the first light, bandwidth, power density or energy density during irradiated tissue.

Organize beauty treatment or the method for Medical Treatment for 142. one kinds, described method comprises and adopts the first illumination to penetrate tissue, and the peak wavelength of the first light is about 400 to about 750nm, and during single treatment, energy density is about 4 to about 60J/cm ², about 10 to about 60J/cm ², about 10 to about 50J/cm ², about 10 to about 40J/cm ², about 10 to about 30J/cm ², about 20 to about 40J/cm ², about 15J/cm ²to 25J/cm ², about 10 to about 20J/cm ².

143. methods according to any one of claim 139 to 141, wherein the average power density of the first light is about 4 to about 75mW/cm ², or about 55mW/cm ²to about 150mW/cm ².

144. methods according to any one of claim 138 to 142, wherein the energy density of the first light single treatment is about 4 to about 60J/cm ², about 10 to about 60J/cm ², about 10 to about 50J/cm ², about 10 to about 40J/cm ², about 10 to about 30J/cm ², about 20 to about 40J/cm ², about 15J/cm ²to 25J/cm ², or about 10 to about 20J/cm ².

145. methods according to any one of claim 138,140 or 142, wherein the bandwidth of the first light is about 15nm to about 100nm, approximately 25nm to about 80nm, approximately 30nm to about 70nm, or about 20nm to about 50nm.

146. methods according to any one of claim 138 to 145, wherein said peak wavelength is about 400nm to about 450nm, approximately 450nm to about 490nm, approximately 490nm to about 560nm, approximately 560nm to about 590nm, or approximately 590nm to about 635nm or approximately 635nm to about 720nm.

147. methods according to any one of claim 142 or 144, wherein single treatment comprise irradiate tissue or light activatable compositions be no more than about 30 minutes, about 1 minute to about 30 minutes, about 1 minute to about 25 minutes, about 1 minute to about 20 minutes, about 1 minute to about 19 minutes, about 1 minute to about 18 minutes, about 1 minute to about 17 minutes, about 1 minute to about 16 minutes, about 1 minute to about 15 minutes, about 1 minute to about 14 minutes, about 1 minute to about 13 minutes, about 1 minute to about 12 minutes, about 1 minute to about 11 minutes, about 1 minute to about 10 minutes, about 1 minute to about 9 minutes, about 1 minute to about 8 minutes, about 1 minute to about 7 minutes, about 1 minute to about 6 minutes, about 1 minute to about 5 minutes, about 1 minute to about 4 minutes, about 1 minute to about 3 minutes.

Organize beauty treatment or the method for Medical Treatment for 148. one kinds, described method comprises:

The incoherent light from the first light source, the about 430nm of peak wavelength to about 500nm is adopted to irradiate tissue;

The incoherent light from secondary light source, the about 400nm of peak wavelength to about 430nm is adopted to irradiate tissue; The overall power density of wherein irradiating the light of tissue is about 4 to about 75mW/cm ², or about 55mW/cm ²to about 150mW/cm ².

149. according to the method described in claim 147, is included in that use organizationally before illumination is penetrated can photoactivatable composition further.

150. methods according to claim 148 or 149, wherein during single treatment, the energy density of the first light and the second light is about 4 to about 60J/cm ², about 10 to about 60J/cm ², about 10 to about 50J/cm ², about 10 to about 40J/cm ², about 10 to about 30J/cm ², about 20 to about 40J/cm ², about 15J/cm ²to 25J/cm ², or about 10 to about 20J/cm ².

151. according to the method described in claim 150, wherein single treatment comprise irradiate tissue or light activatable compositions be no more than about 30 minutes, about 1 minute to about 30 minutes, about 1 minute to about 25 minutes, about 1 minute to about 20 minutes, about 1 minute to about 19 minutes, about 1 minute to about 18 minutes, about 1 minute to about 17 minutes, about 1 minute to about 16 minutes, about 1 minute to about 15 minutes, about 1 minute to about 14 minutes, about 1 minute to about 13 minutes, about 1 minute to about 12 minutes, about 1 minute to about 11 minutes, about 1 minute to about 10 minutes, about 1 minute to about 9 minutes, about 1 minute to about 8 minutes, about 1 minute to about 7 minutes, about 1 minute to about 6 minutes, about 1 minute to about 5 minutes, about 1 minute to about 4 minutes, about 1 minute to about 3 minutes.

152. one kinds of that be substantially described with reference to accompanying drawing and/or device shown in the drawings or lamps.

153. one kinds substantially with reference to that be described and/or the method shown in the drawings of accompanying drawing.