CN105325432A - Application of berberine in preparing marine fouling organism preventing and removing agent - Google Patents
Application of berberine in preparing marine fouling organism preventing and removing agent Download PDFInfo
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- CN105325432A CN105325432A CN201510922203.0A CN201510922203A CN105325432A CN 105325432 A CN105325432 A CN 105325432A CN 201510922203 A CN201510922203 A CN 201510922203A CN 105325432 A CN105325432 A CN 105325432A
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- berberine
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Abstract
The invention discloses application of berberine in preparing a marine fouling organism preventing and removing agent. The structural formula of berberine is shown as the formula (I) in the specification. When a solid surface is coated with berberine at a low dosage, an obvious effect of inhibiting barnacle and mussel larvae from adhering can be achieved, and therefore berberine can be applied to preparing the marine fouling organism preventing and removing agent. In addition, berberine is a naturally existing organic compound and will not pollute the water environment, the situation that berberine is transferred through a food chain to be enriched in organisms will not occur, berberine is environmentally friendly and high in safety, and contains no heavy metal element like copper or tin while marine organisms are effectively inhibited from adhering, and therefore berberine has good social benefits on the perspective of environmental protection. Furthermore, the source of berberine is wide, a separation and preparation means is easy and convenient to implement, and therefore berberine is suitable for large-scale production and large in popularization potential and has good application prospects in preventing and removing marine fouling organisms.
Description
Technical field:
The invention belongs to natural product field, be specifically related to jamaicin and preparing the application in preventing and removing marine fouling organisms agent.
Background technology:
Marine fouling organism refers to set or perches in boats and ships and Artificial facilities underwater sites, all animals that economical activities of mankind is had a negative impact, plant and microorganism, its harm mainly increases boats and ships running resistance, increases fuel consumption, reduce the speed of a ship or plane, affect mobility and the fighting capacity on naval vessel; Blocking waterpipe, hinders and supplies water or lower cooling effect; Change Process of Metallic Corrosion, cause local corrosion or pitting corrosion; Reduce the sensitivity of instrument and rotatable parts, interference Instrument equipment normally runs; Increase the deadweight of marine facility and improve its center of gravity, enlarging markedly the dynamic load(loading) effect caused by wave and ocean current; Cultivate object such as the biologies such as shellfish with economy and fight for adherance and bait, block the mesh of breeding facility, hinder internal and external environment water body to exchange, be even attached directly on cultivation object and disturb its growth and development process and its quality of reduction.
Antifouling paint is in the past released to main path with poison material, by forming concentration of poisons layer algae spores and invertebrate larva to toxic action at body surface, realize fouling organism and prevent and kill off object, therefore unavoidably there is risk and the drawback of the harm marine eco-environment, even also can be healthy by food chain harm humans.Therefore, development of new, efficient, low toxicity, environment-friendly antifouling agent have become current urgent problem and study hotspot.
Because marine fouling organism is made up of animal, plant and microorganism, wherein endanger kind that is comparatively large and that be difficult to remove and mainly have calcium carbonate shell, the stockless cirrus class (barnacle) of battalion's set life and a bivalve (mussel and oyster).Therefore, the work of anti-fouling compound filler test is tested object mainly with stockless cirrus class and Bivalve.
At the torrid zone, subtropics littoral sea, reticulate pattern barnacle is the Typical Representative of stockless cirrus class, and is the kind had comparative advantage in fouling community; Perna viridis is then the common bivalve in the East Sea and the South Sea, is also to be attached to the important fouling organism kind on boats and ships, boat (floating) mark and aquaculture installation.Demonstration test adopts reticulate pattern barnacle and Perna viridis as experimental subjects, and the achievement in research obtained will have representative widely.
Fouling organism is divided into two life stages usually, and deviating from egg membrane from larva and be developed to explore off and on till body surface prepares settlement and metamorphosis, is the life stage that swims; Once larva chooses colonization sites, after adherance surface attachment, the abnormal formation young, then it is set or epiphytism stage.From stained angle, its to the mankind produce harm start from set or attachment after.If effectively suppress the settlement and metamorphosis of larva, the object of preventing and kill off just can be reached.Therefore, adopt the halobiontic larva of this two class be experimental subjects to check the anti-fouling effect of compound, there is scientific rationality and representative meaning.
The antifouling mechanism of action of marine organisms native chemical can be the nuisanceless anti-soil technology of exploitation and offers reference, and marine organisms itself also should be the important sources of novel antifouling agent, but the relevant comparision contents of native compound in marine organism is few, and structure relative complex, be not easy to deep exploitation and extensive use.Therefore, from of a great variety, widely distributed, the terrestrial plant resource easily of drawing materials, find the high-efficiency environment friendly anti-fouling compound being more suitable for applying, will be a kind of brand-new trial, exploration and selection, and there is important theory and realistic meaning.
Jamaicin, also known as berberine, is a kind of common morphinane alkaloid, is extensively present in many plants of four sections ten genus such as Berberidaceae, is obtained first as far back as 1826 by Xia Waliai and Pei Ertan from Xanthoxylonclava bark.Jamaicin used in the present invention extracts from the Ranunculaceae coptis plant coptis (Coptischinensis).Jamaicin has very strong antibacterial action, has multiple pharmacological function.But, in preventing and removing marine fouling organisms field, have no any and prevent and kill off aspect about report is disclosed its anti-attachment effect and uses it for fouling organism.
Jamaicin, English name Berberine is yellow needles, and molecular formula is C
20h
18nO
4 +, molecular weight is 336.36, and chemical structural formula is such as formula shown in (I).
Formula (I).
Summary of the invention:
The object of this invention is to provide jamaicin and prepare the application in preventing and removing marine fouling organisms agent.
Jamaicin of the present invention is 10 μ g/cm at coated weight
2time, the adhesive rate of kentrogon after 72 hours is 22.1%, far below 87.7% of control group, shows that jamaicin produces obvious inhibitory action to kentrogon.Be 1 μ g/cm at jamaicin coated weight
2time, the adhesive rate of Mytilus Edulis Larva is only 5.2%, and well below control group, difference extremely significantly (p<0.01), shows that jamaicin can suppress the attachment of Perna viridis larva effectively.The larva of jamaicin to cirrus class and bivalve is attached with good inhibitory action as can be seen here.
Therefore, the invention provides jamaicin and prepare the application in preventing and removing marine fouling organisms agent.
Described preventing and removing marine fouling organisms agent is preferably kentrogon agent for preventing and eliminating or Mytilus Edulis Larva agent for preventing and eliminating.
Compared with prior art, the present invention has following beneficial effect:
Jamaicin of the present invention, when low dosage is coated on the surface of solids, namely has the effect significantly suppressing marine organisms attachment, therefore, it is possible to for the preparation of preventing and removing marine fouling organisms agent.And jamaicin is naturally occurring organic compound; can not polluted-water environment and cause its enrichment in organism by food chain transmission; environmentally friendly; safety is high; while effectively suppressing marine organisms attachment; the not heavy metal element such as cupric and tin; from environmental protection angle, there is good social benefit; and jamaicin source is very extensive; it is easy that approach is prepared in separation; be suitable for large-scale production, promotion potential is large, has a good application prospect in preventing and removing marine fouling organisms.
Embodiment:
Following examples further illustrate of the present invention, instead of limitation of the present invention.
Embodiment 1:
Experimental group: dissolved by jamaicin distilled water, compound concentration is the solution of 28.26 μ g/ml.Be add this solution of 1ml in the culture dish of 6cm at diameter, and make bottom its uniform fold culture dish.After solvent volatilizees completely, the dosage being coated on the jamaicin bottom culture dish is 10 μ g/cm
2.Add 10ml seawater.
Control group: add 1ml distilled water, makes it be uniformly distributed in bottom culture dish, to be evaporatedly adds 10ml seawater completely again.
Blank group: add 10ml seawater.
Experimental group, blank group and control group respectively establish 5 Duplicate Samples.30 reticulate pattern barnacle cyprids are added respectively in each sample.Being placed in temperature is cultivate in dark surrounds in the constant incubator of about 29 DEG C.Observed once every 24 hours.Cultivate attachment final to each group of larva after 72 hours and death state carries out statistical analysis.
Table 1 lists attachment and the lethality of experimental group, control group and blank group cyprids.Visible, cultivate in constant incubator after 72 hours, the larva adhesive rate of blank group is 89.8%, control group 87.7%, the adhesive rate of blank group and control group cyprids, without significant difference (p>0.05), can not leave over the harmful substance affecting cyprids activity after showing the distilled water volatilization as solvent.As for the experimental group covering jamaicin bottom culture dish, the adhesive rate of its cyprids is 22.1%, is far smaller than control group, and difference extremely significantly (p<0.01), shows that jamaicin is at 10 μ g/cm
2dosage under effectively can suppress the attachment of reticulate pattern barnacle cyprids.
Table 1: reticulate pattern barnacle cyprids attachment
Embodiment 2
Experimental group: dissolved by jamaicin distilled water, compound concentration is the solution of 28.26 μ g/ml.Be add this solution of 1ml in the culture dish of 6cm and make bottom its uniform fold culture dish at diameter.After solvent volatilizees completely, the content of berberine be coated on bottom culture dish is 1.0 μ g/cm
2.Add 10ml seawater.
Control group: add 1ml distilled water, makes dissolution homogeneity be distributed in bottom culture dish, treats solvent volatilization completely, then adds 10ml seawater.
Blank group: add 10ml seawater.
The determination of larva quantity: get the water body containing Perna viridis veliger from nursery pond and concentrate with bolting silk.Point respectively get 50ml three times, instillation 1-2 drips formalin solution and kills in counted under microscope after larva, and averaging of income value is the density of larva in water body.
Experimental group, blank group and control group all establish 5 Duplicate Samples, add about 30 Perna viridis larvas in each sample.Be about in temperature in the incubator of 26 DEG C and cultivate under dark surrounds.Observed once every 24 hours.Cultivate attachment final to each group of Perna viridis larva after 72 hours and death state carries out statistical analysis.
Table 2 lists experimental group, control group and blank attachment and the lethality organizing Perna viridis larva.Visible, cultivate in constant incubator after 72 hours, the larva adhesive rate of blank group is about 36.5%, control group about 35.2%, the adhesive rate of blank group and control group veliger, without significant difference (p>0.05), can not leave over the harmful substance affecting veliger activity after showing to imitate volatilization as the distilled water of solvent.And through the experimental group of jamaicin process, the adhesive rate of its larva is only 5.2%, well below control group, difference extremely significantly (p<0.01), shows that this compound can suppress the attachment of Perna viridis larva effectively.Table 2: Perna viridis veliger attachment
| Group | Proof load | Adhesive rate % |
| Blank group | － | 36.5 |
| Control group | － | 35.2 |
| Experimental group | 1.0μg/cm 2 | 5.2 |
Claims (2)
1. the application in preventing and removing marine fouling organisms agent prepared by jamaicin, and the structural formula of described jamaicin is such as formula shown in (I):
2. application according to claim 1, is characterized in that, described preventing and removing marine fouling organisms agent is kentrogon agent for preventing and eliminating or Mytilus Edulis Larva agent for preventing and eliminating.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108056113A (en) * | 2017-12-28 | 2018-05-22 | 中国科学院南海海洋研究所 | Sargassum henslowianum acetic acid ethyl ester extract separation component E and preparation method thereof and the application in preventing and removing marine fouling organisms |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102715175A (en) * | 2012-07-04 | 2012-10-10 | 天津农学院 | Berberine nanoemulsion algicide and preparation method of berberine nanoemulsion algicide |
| CN102771484A (en) * | 2012-07-04 | 2012-11-14 | 天津农学院 | Berberine.artemisinin composite algicide |
| US20140114231A1 (en) * | 2012-10-20 | 2014-04-24 | Bertha Rostro | Formulations and Compositions of Hydrophobic Extracts of Skin Tree |
| CN104145992A (en) * | 2014-07-24 | 2014-11-19 | 温文 | Algae-killing composition for water body |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102715175A (en) * | 2012-07-04 | 2012-10-10 | 天津农学院 | Berberine nanoemulsion algicide and preparation method of berberine nanoemulsion algicide |
| CN102771484A (en) * | 2012-07-04 | 2012-11-14 | 天津农学院 | Berberine.artemisinin composite algicide |
| US20140114231A1 (en) * | 2012-10-20 | 2014-04-24 | Bertha Rostro | Formulations and Compositions of Hydrophobic Extracts of Skin Tree |
| CN104145992A (en) * | 2014-07-24 | 2014-11-19 | 温文 | Algae-killing composition for water body |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108056113A (en) * | 2017-12-28 | 2018-05-22 | 中国科学院南海海洋研究所 | Sargassum henslowianum acetic acid ethyl ester extract separation component E and preparation method thereof and the application in preventing and removing marine fouling organisms |
| CN108056113B (en) * | 2017-12-28 | 2020-05-19 | 中国科学院南海海洋研究所 | Component E separated from sargassum henryi ethyl acetate extract, preparation method thereof and application of component E in marine fouling organism prevention and removal |
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