CN105306215A - Unattended transformer station - Google Patents
Unattended transformer station Download PDFInfo
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- CN105306215A CN105306215A CN201510726085.6A CN201510726085A CN105306215A CN 105306215 A CN105306215 A CN 105306215A CN 201510726085 A CN201510726085 A CN 201510726085A CN 105306215 A CN105306215 A CN 105306215A
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Abstract
The invention relates to an unattended transformer station. The unattended transformer station comprises a transformer station end and a key end, and is characterized in that the transformer station end comprises a verification apparatus, a decryption apparatus and a transformer station end wireless apparatus, and the key end comprises an encryption apparatus and a key end wireless apparatus. According to the technical scheme provided by the embodiment of the invention, identify information is verified by use of a micro-fluidic chip, randomized numbers are obtained by use of a hardware mechanism and encryption is carried out by use of an image mechanism, such that the security is quite high, the operation speed is also very fast, and certain error codes can also be tolerated.
Description
Technical field
The application relates to power domain, particularly relates to a kind of unattended operation transformer station.
Background technology
Transformer station, changes the place of voltage.In order to the power delivery that power plant is sent is to place far away, voltage must be raised, become high-tension electricity, on demand voltage be reduced near user again, the work of this buck/boost has come by transformer station.The capital equipment of transformer station is switch and transformer.Different by scale, little is called electric substation.Transformer station is greater than electric substation.Transformer station is voltage of transformation in electric power system, acceptance and distribution electric energy, the flow direction of control electric power and the electric power facility of adjustment voltage, and it is got up by the grid contact of its transformer by each step voltage.
At present, the transformer station in electric power system achieves unattended substantially, and the operation maintenance of transformer station have employed centralized control center and adds unwatched pattern.Transformer station's distribution often compares dispersion, and the door lock of its gate, control room and switch room etc. adopts mechanical lock, the numerous use of key and difficult management usually.
Summary of the invention
For overcoming Problems existing in correlation technique, the application provides a kind of unattended transformer station.
In the embodiment of the application, provide a kind of unattended operation transformer station, it comprises transformer substation end and key end, and transformer substation end comprises demo plant, decryption device and transformer substation end wireless device, and key end comprises encryption device and key end wireless device;
Encryption device comprises:
Micro-fluidic chip detection module, for obtaining initial data string A
0={ a
n, wherein, a is initial data string A
0in character, n is the figure place of a;
Micro-fluidic chip detection module is a kind of DNA detection module, comprising: transducing signal detection zone, transducing signal mixed zone and transducing signal output area.
Transducing signal detection zone is mainly used to obtain the primary sample of user, and transducing signal mixed zone is mainly used in nano-sensor and detects and fully the mixing of sample, and enters into transducing signal detection zone after mixing is complete; Transducing signal detection zone is mainly used in the detection of the fluorescence signal produced after nano-sensor detects target gene; Detect complete data and obtain initial data string A
0,
Described micro-fluidic chip, overall dimensions is: wide 30mm, high 20mm, dark 90 μm, the wide 24mm in structural core district, high 16mm; Micro-fluidic chip forms by 4 layers, ground floor substrate: glass, second and third layer: ultra-violet curing glue, 4th layer: mask, each interlayer adhesive: NOA81 optical cement, adopt plasma oxidation bonding method to make substrate and the sealing-in of ground floor structure, the chip structure of each layer is made up of photoetching process;
Matrixing module, for by initial data string A
0matrixing obtains matrix A={ a
ijah × aw, specifically comprise: to { a
nfrom head of the queue get successively i ?1 character, when odd-times gets character, the character string obtained string head mend 10, supply as a line a
i, when even-times gets character, mend 11 at the string tail of the character string obtained, supply as a line a
i, for the character string obtained for the last time, then first open from string and begin to mend 10 every a character, until supply as a line a
i, by all a obtained
imatrix A={ a is obtained according to the pseudo-random sequence arrangement preset
ijah × aw, wherein, ah is the height of matrix A, and aw is the height of matrix A;
First noise module, the last position of the amplitude that the shortwave recording key end wireless device monitors predeterminated frequency in real time obtains forms the first noise data sequence C T1 by odd number fetch bit;
First scrambling module, for from first element of matrix A, gets the first random function R (1) individual element successively and is inserted in matrix A and obtains matrix B={ b from the first noise data sequence C T1
stbh × bw,
Wherein, bh is the height of matrix B, and bw is the height of matrix B, first random function R (1)=CT1
s%64, CT1
sfor the number obtained successively from the first noise data sequence C T1;
The figure place of noise data makes s be 32 times of i, and t is 32 times of j;
3rd noise module, the last position of the amplitude that the shortwave recording key end wireless device monitors predeterminated frequency in real time obtains forms the 3rd noise data sequence C T3 by even number fetch bit;
3rd scrambling module, for matrix B and the 3rd noise data sequence C T3 are carried out XOR, obtains enciphered data: Matrix C={ c
stch × cw, ch be the height of Matrix C, cw is the height of Matrix C;
Transformer substation end wireless device and key end wireless device all for monitoring the wireless short wave of identical predeterminated frequency, to obtain the first noise sequence CT1 and the 3rd noise sequence CT3;
Decryption device is used for the noise data from transformer substation end wireless device with from the second noise sequence CT2 that transformer substation end prestores, and the enciphered data from encryption device is performed to the inverse operation of the cryptographic calculation of above-mentioned encryption device;
Demo plant is used for decryption device to compare to the initial data that the enciphered data from encryption device carries out data that inverse operation obtains and transformer substation end, if comparison coincidence rate exceedes preset value, then confirms as and is verified.
User is in advance with the identity information of the micro-fluidic chip detection module registration oneself of saliva in encryption device of oneself, later in routine use process, when user is near transformer station, user only need use key end collection self saliva a small amount of, authorization information is sent to transformer substation end after the micro-fluidic chip effect identity information of key end, after comparison confirms, identity can be confirmed, enter transformer station smoothly, namely adopt the transformer station of the program can uniquely determine user's identity according to user DNA, there is very large fail safe.
The technical scheme that the embodiment of the application provides have employed micro-fluidic chip to verify identity information, adopt hardware mechanisms to obtain random number, have employed image mechanism to be encrypted, thus existing very high fail safe, there is again very fast arithmetic speed, and certain error code can also be allowed.
The aspect that the application adds and advantage will part provide in the following description, and part will become obvious from the following description, or be recognized by the practice of the application.Should be understood that, it is only exemplary and explanatory that above general description and details hereinafter describe, and can not limit the application.
Accompanying drawing explanation
Accompanying drawing to be herein merged in specification and to form the part of this specification, shows embodiment according to the invention, and is used from specification one and explains principle of the present invention.
Fig. 1 is the device block diagram of a kind of unattended transformer station according to an exemplary embodiment;
Fig. 2 is the device block diagram of the encryption device according to an exemplary embodiment;
Fig. 3 is the device block diagram of the encryption device according to another exemplary embodiment;
Fig. 4 be based on the nano biological sensor of graphene oxide to the gene principle of the specific detection of gene: after the single strand dna probe being modified with fluorophor FAM contacts with graphene oxide, graphene oxide absorption single stranded DNA, and cause FAM Quenching of fluorescence; After adding target gene, due to base pair complementarity principle, form double-stranded DNA, and come off from surface of graphene oxide, recover fluorescence, thus reach the object detecting target gene;
Fig. 5 is the course of work schematic diagram of automation micro-fluidic chip of the present invention;
Fig. 6 is the structural representation of automation micro-fluidic chip of the present invention.
Embodiment
Here will be described exemplary embodiment in detail, its sample table shows in the accompanying drawings.When description below relates to accompanying drawing, unless otherwise indicated, the same numbers in different accompanying drawing represents same or analogous key element.Execution mode described in following exemplary embodiment does not represent all execution modes consistent with the present invention.On the contrary, they only with as in appended claims describe in detail, the example of apparatus and method that aspects more of the present invention are consistent.
Disclosing hereafter provides the different structure that many different embodiments or example are used for realizing the application.In order to simplify disclosing of the application, hereinafter the parts of specific examples and setting are described.Certainly, they are only example, and object does not lie in restriction the application.In addition, the application can in different example repeat reference numerals and/or letter.This repetition is to simplify and clearly object, itself just institute the relation between various embodiment and/or setting is discussed.In addition, this application provides the example of various specific technique and material, but those of ordinary skill in the art can recognize the applicability of other techniques and/or the use of other materials.In addition, fisrt feature described below Second Eigenvalue " on " structure can comprise the embodiment that the first and second features are formed as directly contact, also can comprise other feature and be formed in embodiment between the first and second features, such first and second features may not be direct contacts.
In the description of the application, it should be noted that, unless otherwise prescribed and limit, term " installation ", " being connected ", " connection " should be interpreted broadly, such as, can be mechanical connection or electrical connection, also can be the connection of two element internals, can be directly be connected, also indirectly can be connected by intermediary, for the ordinary skill in the art, the concrete meaning of above-mentioned term can be understood as the case may be.
Fig. 1 is the device block diagram of a kind of unattended transformer station according to an exemplary embodiment, it comprises transformer substation end 100 and key end 200, transformer substation end 100 comprises demo plant 130, decryption device 120 and transformer substation end wireless device 110, and key end 200 comprises encryption device 220 and key end wireless device 210.
Fig. 2 is the device block diagram of the encryption device 220 according to an exemplary embodiment, and encryption device 220 comprises:
Micro-fluidic chip detection module 12, for obtaining initial data string A
0={ a
n, wherein, a is initial data string A
0in character, n is the figure place of a;
Micro-fluidic chip detection module is a kind of DNA detection module, comprising: transducing signal detection zone, transducing signal mixed zone and transducing signal output area.
Transducing signal detection zone is mainly used to obtain the primary sample of user, and transducing signal mixed zone is mainly used in nano-sensor and detects and fully the mixing of sample, and enters into transducing signal detection zone after mixing is complete; Transducing signal detection zone is mainly used in the detection of the fluorescence signal produced after nano-sensor detects target gene; Detect complete data and obtain initial data string A
0,
Described micro-fluidic chip, overall dimensions is: wide 30mm, high 20mm, dark 90 μm, the wide 24mm in structural core district, high 16mm; Micro-fluidic chip forms by 4 layers, ground floor substrate: glass, second and third layer: ultra-violet curing glue, 4th layer: mask, each interlayer adhesive: NOA81 optical cement, adopt plasma oxidation bonding method to make substrate and the sealing-in of ground floor structure, the chip structure of each layer is made up of photoetching process;
Matrixing module 14, for by initial data string A
0matrixing obtains matrix A={ a
ijah × aw, specifically comprise: to { a
nfrom head of the queue get successively i ?1 character, when odd-times gets character, the character string obtained string head mend 10, supply as a line a
i, when even-times gets character, mend 11 at the string tail of the character string obtained, supply as a line a
i, for the character string obtained for the last time, then first open from string and begin to mend 10 every a character, until supply as a line a
i, by all a obtained
imatrix A={ a is obtained according to the pseudo-random sequence arrangement preset
ijah × aw, wherein, ah is the height of matrix A, and aw is the height of matrix A;
First noise module 22, the last position of the amplitude that the shortwave that record key end wireless device 210 monitors predeterminated frequency in real time obtains forms the first noise data sequence C T1 by odd number fetch bit;
First scrambling module 24, for from first element of matrix A, gets the first random function R (1) individual element successively and is inserted in matrix A and obtains matrix B={ b from the first noise data sequence C T1
stbh × bw,
Wherein, bh is the height of matrix B, and bw is the height of matrix B, first random function R (1)=CT1
s%64, CT1
sfor the number obtained successively from the first noise data sequence C T1;
The figure place of noise data makes s be 32 times of i, and t is 32 times of j;
3rd noise module 52, the last position of the amplitude that the shortwave that record key end wireless device 210 monitors predeterminated frequency in real time obtains forms the 3rd noise data sequence C T3 by even number fetch bit;
3rd scrambling module 54, for matrix B and the 3rd noise data sequence C T3 are carried out XOR, obtains enciphered data: Matrix C={ c
stch × cw, ch be the height of Matrix C, cw is the height of Matrix C.
Transformer substation end wireless device 110 and key end wireless device 210 all for monitoring the wireless short wave of identical predeterminated frequency, to obtain the first noise sequence CT1 and the 3rd noise sequence CT3;
Decryption device 120, for the noise data from transformer substation end wireless device 110 with from the second noise sequence CT2 that transformer substation end prestores, performs the inverse operation of the cryptographic calculation of above-mentioned encryption device 220 to the enciphered data from encryption device 220;
Demo plant 130, for being compared to the initial data that the enciphered data from encryption device 220 carries out data that inverse operation obtains and transformer substation end by decryption device 120, if comparison coincidence rate exceedes preset value, is then confirmed as and is verified.
Encryption mechanism more complicated of the present invention, realizes random sequence by hardware, therefore has the fail safe of height.
DNA is as the basic genetic material of biology, and the detection of DNA has major application to be worth in fields such as biotechnology, environmental protection, life sciences.The research of DNA sensor is at home and abroad paid close attention to widely.Surface plasmon resonance (SurfacePlasmonResonance, SPR) be metal surface phasmon Wave coupling irradiate light produce a kind of covibration, the change of metal film surfaces dielectric constant and thickness can affect the change of SPR resonance curve, thus realizes the sensing of metal surface media environment change.Have in real time, original position and exempt from mark detect, detect quick, highly sensitive, the features such as detectable limit (limitedofdetection, LOD) is low, are mainly used in the research application in the fields such as medicine, biology, chemistry, food at present, mark can be exempted from and detect protein, the materials such as DNA.But the method due to traditional detection single stranded DNA is at SPR chip surface stationary probe single stranded DNA (ssDNA) molecule, utilizes base sequence matching process to catch target single stranded DNA in sample solution, thus realize testing goal.
Graphene within 2004, finds and a kind of new material developed rapidly, is widely used in the fields such as medicine, biology, chemistry, electronics, physics.Graphene is oxidized can form graphene oxide (GrapheneOxide, GO), surface is with the oxygen-containing functional group such as carboxyl, hydroxyl, can be water-soluble, can be interacted and stable bond by hydrogen bond and pi-pi bond with ssDNA, adhesion is much larger than the adhesion of double-stranded DNA (dsDNA) and GO.
The design of micro-fluidic chip of the present invention is mainly for some consideration following:
Biology sensor is the analysis device using biomaterial or derivatives thereof as molecular recognition elements, has the advantages such as selectivity is strong, highly sensitive, analytic process is easy; Microfluid based Lab on a chip is the unified platform with laboratory operation comprehensive functions such as mixing, separation, reaction, detections built on the chip of several square centimeters, has the advantages such as microminiaturization, integrated, automation, the low consumption of the reagent energy.
Microfluid based Lab on a chip, be also called micro-fluidic chip (MicrofluidicChip) or chip lab (LabonaChip), refer to the microminiaturization built on the chip of several square centimeters or less, integrated, the chemistry of automation, biological experiment platform figure.Micro-fluidic chip rises yardstick manipulation fluid at microlitre or receive, and makes interaction between substances, thus realizes the transmission of material, energy, information, reach required physics, chemistry or biological effect.
DNA biosensor
To refer to biomaterial and derivative thereof as molecular recognition elements, by inverting element, specified measuring range is converted to device or the device A biology sensor of usable output signal.
DNA biosensor is exactly the molecular recognition elements using molecule as biology sensor, and molecular recognition behaviorism theory is the device of the detectable signal such as optics, electrochemistry by inverting element.According to the difference of molecular recognition process, be referred to as sense stricto biology sensor by what undertaken identifying by base pair complementarity principle, be mainly used in the detection of gene molecule; The oligonucleotide chain (aptamer) being carried out specific recognition by space multistory conformation is referred to as aptamer biology sensor, the detection of chemical small molecule, protein and other, even cell can be used for.
FRET technology
FRET (Fluorescenceresonanceenergytransfer, FRET) be a kind of Fluorescence-quenching relying on distance, reason is in two different fluorescence molecules, there is overlapping phenomenon in the wavelength of the absorption spectra of the emission spectra of one of them fluorescence molecule and another fluorescence molecule, when reaching suitable distance therebetween, be in the energizing quantity illuminophore of excitation state, by radiationless dipolar interaction, energy transferring be subject to energy group to suitable.Mainly there are two kinds of mechanisms of action in FRET: the Resonance energy transfer one, between the singlet state and the singlet state being subject to energy body of energizing quantity body, and the Main Function mechanism being, plays a leading role in the distance of 1nm to 10nm at the two; Two, the Resonance energy transfer between the triplet and the singlet state being subject to energy body of energizing quantity body, effective range is 1nm to 1.5nm.
There are demand fulfillment four conditions in FRET: one, energy donor group and energy acceptor group distance are no more than 10nm; Two, the emission spectra wave-length coverage of energy donor group and the excitation spectrum wave-length coverage of energy acceptor group can be effectively overlapping; Three, the fluorescence quantum yield of energy donor is enough high; Four, the dipole that energy donor group and acceptor are rolled into a ball has suitable relative bearing orientation.
Nano biological sensor
Broadly, natural or the artificial device that nanoscale plays bio-sensing function all can be classified as nano biological sensor, as the bio-sensing process etc. that the natural transducer of the various nanoscale structures in organism and molecule machine, the molecular beacon of engineer, various nano material participate in.In the narrow sense, nano biological sensor refer to nano material and biomaterial jointly complete identify, the analysis device of the quantitative output procedure of transducing, signal.
Aptamer
Aptamer (Aptamer) refers to utilization index enrichment Fas lignand system evolution (SystematicEvolutionofLigandsbyExponentialenrichment, SELEX) technology, from the oligonucleotide library of Prof. Du Yucang screening obtain can with the oligonucleotide chain of target molecule specific bond.Aptamer can pass through G-C, A-T (or A-U) base pairing and form the multiple space structures such as stem ring, hair clip, pocket, G-tetra-serobila.When interacting with target molecule, can be changed by adaptive induced-fit and fold, form stable compound with target molecule.The identification combination of aptamer and target molecule contains the complementation of conformation shape, the sintering action of aromatic, the electrostatic interaction of charged group and hydrogen bond action etc.
Fig. 4 illustrates nano biological sensor based on graphene oxide to the gene principle of the specific detection of gene: after the single strand dna probe being modified with fluorophor FAM contacts with graphene oxide, graphene oxide absorption single stranded DNA, and cause FAM Quenching of fluorescence; After adding target gene, due to base pair complementarity principle, form double-stranded DNA, and come off from surface of graphene oxide, recover fluorescence, thus reach the object detecting target gene.
The molecular mechanism of this process is electronic structure based on the uniqueness of graphene oxide and chemical property: one, graphene oxide has strong suction-operated to single stranded DNA, but but very weak to the absorption of double-strand.This is because graphene oxide π-pi-conjugated effect has strong suction-operated to base and aromatic compound, and then absorption is produced to single stranded DNA; In contrast, the membership that adds of complementary dna chain changes the conformation of strand, and has a large amount of negative electrical charges due to dsdna epitope mask, produces and repels, thus be unfavorable for the absorption of graphene oxide to DNA double chain with the same graphene oxide covering negative electrical charge.Two, graphene oxide is to the quenching effect of fluorescence molecule.Graphene oxide owing to having the domain of sp2 hydridization, when its with fluorescence molecule in conjunction with time, there is FRET (FRET), thus quench fluorescence.
Primary sample mainly takes from the body fluid of user, such as saliva etc.DNA technique and Graphene technology use in the middle of encryption by the present embodiment, achieve authentication, achieve unexpected effect in reinforcing security, and on encryption efficiency, also allow people unexpected.
The present invention first noise sequence and the 3rd noise sequence all come from hardware interface, substantially do not need computing to generate, so very save operational capability, make encrypting and decrypting have very fast arithmetic speed.
For the embodiment of Fig. 2, inventor improves, and devises following preferred embodiment.
Preferably, described micro-fluidic chip forms by 4 layers, and ground floor is substrate, prevents leakage; The second layer by mixed zone, feed liquor district, go out liquid zone composition, third layer is detection zone and the trapezoidal shape connector being connected each region, and the 4th layer is mask; Plasma oxidation bonding method is adopted to make substrate and the sealing-in of ground floor structure; The chip structure of each layer is made up of photoetching process.
Preferably, described micro-mixer district has micro-mixer passage, and it is configured to W shape passage; The width of described W shape passage is 350 μm, and the angle at turning is 90 degree, totally 6 corner unit; The width of convex ridge is 50 μm, and the angle at turning is 120 degree, and in Z-shaped passage, each straight passage portion has 5 parallel convex ridges, is staggered at the convex ridge of adjacency channel.
Preferably, detection liquid is simultaneously joined micro-fluidic chip sample introduction zone from different injection port by miniflow pump from sample liquid; Two kinds of solution are mixed fully by micro-mixer, detect after hatching 20min; After detection, waste liquid is discharged by outlet, completes whole testing process; Utilize fluorescence microscope CCD lighting apparatus to carry out collection and the record of fluorescence signal, after image processing software ImageJ process, obtain the luminous intensity quantitative data of fluorescence.
Preferably, described detection liquid comprises amination graphene oxide concentration and DNA molecular probe, also comprises POSSFF; Further preferably, the malic acid of 0.5g/L is also comprised; Described DNA molecular probe is: Multi-Objective Genetic fragment-specific primer synthesizes dsDNA by gene clone technology, dsDNA fragment, with sybergreen non-covalent labeling phycoerythrin (PE).In the middle of practical application of the present invention, the conveniently identity of authentication of user, DNA detection can be carried out with the body fluid of user (such as saliva) in advance and realize identity registration, also can register the DNA information of several user in advance, thus facilitate several user's communal substation.
Above preferred embodiment all improves the structure of micro-fluidic chip and manufacture craft, thus obtained fluidic chip detecting system sensitivity is very high.
Fig. 3 is the device block diagram of the encryption device 220 according to an exemplary embodiment, and encryption device 220 comprises:
Micro-fluidic chip detection module 12, for obtaining initial data string A
0={ a
n, wherein, a is initial data string A
0in character, n is the figure place of a;
Micro-fluidic chip detection module is a kind of DNA detection module, comprising: transducing signal detection zone, transducing signal mixed zone and transducing signal output area.
Transducing signal detection zone is mainly used to obtain the primary sample of user, and transducing signal blender district is mainly used in nano-sensor and detects and fully the mixing of sample, and enters into transducing signal detection zone after mixing is complete; Transducing signal detection zone is mainly used in the detection of the fluorescence signal produced after nano-sensor detects target gene; Detect complete data and obtain initial data string A
0,
Described micro-fluidic chip, overall dimensions is: wide 30mm, high 20mm, dark 90 μm, the wide 24mm in structural core district, high 16mm; Micro-fluidic chip forms by 4 layers, ground floor substrate: glass, second and third layer: ultra-violet curing glue, 4th layer: mask, each interlayer adhesive: NOA81 optical cement, adopt plasma oxidation bonding method to make substrate and the sealing-in of ground floor structure, the chip structure of each layer is made up of photoetching process;
Matrixing module 14, for by initial data string A
0matrixing obtains matrix A={ a
ijah × aw, specifically comprise: to { a
nfrom head of the queue get successively i ?1 character, when odd-times gets character, the character string obtained string head mend 10, supply as a line a
i, when even-times gets character, mend 11 at the string tail of the character string obtained, supply as a line a
i, for the character string obtained for the last time, then first open from string and begin to mend 10 every a character, until supply as a line a
i, by all a obtained
imatrix A={ a is obtained according to the pseudo-random sequence arrangement preset
ijah × aw, wherein, ah is the height of matrix A, and aw is the height of matrix A;
First noise module 22, the last position of the amplitude that the shortwave that record key end wireless device 210 monitors predeterminated frequency in real time obtains forms the first noise data sequence C T1 by odd number fetch bit;
First scrambling module 24, for from first element of matrix A, gets the first random function R (1) individual element successively and is inserted in matrix A and obtains matrix B={ b from the first noise data sequence C T1
stbh × bw,
Wherein, bh is the height of matrix B, and bw is the height of matrix B, first random function R (1)=CT1
s%64, CT1
sfor the number obtained successively from the first noise data sequence C T1;
The figure place of noise data makes s be 32 times of i, and t is 32 times of j;
Color separation module 32, for matrix B being considered as the data bitmap of 8 YMG color spaces, decomposes the gray-scale map matrix Y obtaining Y space, the gray-scale map matrix M in M space, the gray-scale map matrix G in G space;
Second noise module 42, for reading the second noise sequence CT2 that key end prestores;
Second scrambling module 44, for performing following Scrambling Operation to matrix Y, M, G respectively,
(1) for matrix Y, if bin
1i ()=1, then perform
Ex1 (u, v)=(mod ((a+u), M)+1, v), otherwise remains unchanged,
Obtain matrix Y ';
(2) for matrix M, if bin
2i ()=1, then perform
Ex2 (v, u)=(u, mod ((b+v), N)+1), otherwise remain unchanged,
Obtain matrix M ';
(3) for matrix G, be first divided into multiple sub-block and numbered, if bin
3i ()=1, then perform
Ex3 (w)=mod ((c+w), M × N/t)+1, otherwise remain unchanged,
Obtain matrix G ';
Wherein,
U, v are abscissa and the ordinate of pixel in matrix Y, M respectively, and matrix G is divided into multiple sub-block equably, w is the numbering of each sub-block, and t is the size of each sub-block, M and N is the wide and high of matrix Y, M, G respectively, a and b is default parameter, and i is the numbering of current pixel in matrix Y, M, bin
ki () is the i-th number obtained from the second noise sequence CT2, k=1,2,3;
Merge module 34, for by matrix Y ' according to preset the second random function R (2) be inserted into matrix M ' in obtain matrix Y ' M ', then matrix G ' is inserted in matrix Y ' M ' according to the 3rd random function R (3) preset and obtains matrix S, wherein
Second random function R (2)=CT2
s, CT2
sfor the number obtained successively from matrix Y ', if CT2
s=0, then by CT2
sbe inserted into matrix M ' middle CT2
sbefore the number of correspondence position, if CT2
s=1, then by CT2
sbe inserted into matrix M ' middle CT2
safter the number of correspondence position,
3rd random function R (3)=CT3
s, CT3
sfor the number obtained successively from matrix G ', if CT3
s=0, then by CT3
sbe inserted into CT3 in matrix Y ' M '
safter the number of correspondence position × 2, if CT3
s=1, then by CT3
sbe inserted into matrix M ' middle CT3
sbefore the number of correspondence position × 2;
3rd noise module 52, the last position of the amplitude that the shortwave that record key end wireless device 210 monitors predeterminated frequency in real time obtains forms the 3rd noise data sequence C T3 by even number fetch bit;
3rd scrambling module 54, for matrix S and the 3rd noise data sequence C T3 are carried out XOR, obtains enciphered data: Matrix C={ c
stch × cw, ch be the height of Matrix C, cw is the height of Matrix C.
Transformer substation end wireless device 110 and key end wireless device 210 all for monitoring the wireless short wave of identical predeterminated frequency, to obtain the first noise sequence CT1 and the 3rd noise sequence CT3;
Decryption device 120, for the noise data from transformer substation end wireless device 110 with from the second noise sequence CT2 that transformer substation end prestores, performs the inverse operation of the cryptographic calculation of above-mentioned encryption device 220 to the enciphered data from encryption device 220;
Demo plant 130, for being compared to the initial data that the enciphered data from encryption device 220 carries out data that inverse operation obtains and transformer substation end by decryption device 120, if comparison coincidence rate exceedes preset value, is then confirmed as and is verified.
For the embodiment of Fig. 3, inventor improves, and devises following preferred embodiment.
Preferably, described micro-fluidic chip forms by 4 layers, and ground floor is substrate, prevents leakage; The second layer by mixed zone, feed liquor district, go out liquid zone composition, third layer is detection zone and the trapezoidal shape connector being connected each region, and the 4th layer is mask; Plasma oxidation bonding method is adopted to make substrate and the sealing-in of ground floor structure; The chip structure of each layer is made up of photoetching process.
Preferably, described micro-mixer district has micro-mixer passage, and it is configured to W shape passage; The width of described W shape passage is 350 μm, and the angle at turning is 90 degree, totally 6 corner unit; The width of convex ridge is 50 μm, and the angle at turning is 120 degree, and in Z-shaped passage, each straight passage portion has 5 parallel convex ridges, is staggered at the convex ridge of adjacency channel.
Preferably, detection liquid is simultaneously joined micro-fluidic chip sample introduction zone from different injection port by miniflow pump from sample liquid; Two kinds of solution are mixed fully by micro-mixer, detect after hatching 20min; After detection, waste liquid is discharged by outlet, completes whole testing process; Utilize fluorescence microscope CCD lighting apparatus to carry out collection and the record of fluorescence signal, after image processing software ImageJ process, obtain the luminous intensity quantitative data of fluorescence.
Preferably, described detection liquid comprises amination graphene oxide concentration and DNA molecular probe, also comprises POSSFF; Further preferably, the malic acid of 0.5g/L is also comprised; Described DNA molecular probe is: Multi-Objective Genetic fragment-specific primer synthesizes dsDNA by gene clone technology, dsDNA fragment, with sybergreen non-covalent labeling phycoerythrin (PE).
Above preferred embodiment all improves the structure of micro-fluidic chip and manufacture craft, thus obtained fluidic chip detecting system sensitivity is very high.
Inventor goes back PRACTICE OF DESIGN following examples in the design of micro-fluidic chip.
Embodiment 1 builds fluidic chip detecting system:
Fluidic chip detecting system, this system detecting device is micro-fluidic chip, and it comprises:
Eight passage automatic injection pump mixer districts, transducing signal detection zone and go out liquid zone;
Nano-sensor is detected liquid and sample liquid adding on micro-fluidic chip, with eight passage automatic injection pumps for feed liquor power enters into micro-mixer district through microchannel;
Micro-mixer district is mainly used in nano-sensor and detects fully mixing of liquid and sample, enters into transducing signal detection zone after mixing is complete;
Transducing signal detection zone is mainly used in the detection of the fluorescence signal produced after nano-sensor detects target gene, enters fluid district after having detected;
Detect complete sample and discharge micro-fluidic chip with detection liquid waste liquid from Chu Yang district, so far whole testing process terminates;
Micro-fluidic chip overall dimensions is less, wide 30mm, high 20mm, dark 90 μm, the wide 24mm in structural core district, high 16mm, injection port outlet diameter is 700 μm, the microchannel width in sample introduction zone, Ji Chuyang district of microring array district is 350 μm, and detection zone microchannel width is 230 μm.
Described micro-fluidic chip forms by 4 layers, and ground floor is substrate, prevents leakage; The second layer by mixed zone, feed liquor district, go out liquid zone composition, third layer is detection zone and the trapezoidal shape connector being connected each region, and the 4th layer is mask;
Ground floor substrate: glass
Second and third layer: ultra-violet curing glue
4th layer: mask
Each interlayer adhesive: NOA81 optical cement
Plasma oxidation bonding method is adopted to make substrate and the sealing-in of ground floor structure
The chip structure of each layer is made up of photoetching process;
Described micro-mixer channels designs becomes W shape passage, and the passage of this configuration, owing to there is a lot of turning, can increase and also stay the backflow along for channel direction to affect, promote the mixing of solution after phegma cross-current, strengthens the mixing efficiency of micro-fluidic chip solution;
The width of described W shape passage is 350 μm, and the angle at turning is 90 degree, totally 6 corner unit; The width of convex ridge is 50 μm, and the angle at turning is 120 degree, and in Z-shaped passage, each straight passage portion has 5 parallel convex ridges, is staggered at the convex ridge of adjacency channel;
Embodiment 2 prepares native system detection zone detection liquid used and molecular probe:
Native system detection zone detection liquid used comprises amination graphene oxide concentration and molecular probe, also comprises POSSFF; Further preferably, the malic acid of 0.5g/L is also comprised.
The optimum that the present invention determines nano biological sensor amination graphene oxide and fluorescent molecular probe forms, increase amino group, thus increase the water-soluble of organic carrier, and increase carrier surface negative charge amount, single strand dna probe is combined with carrier more stable, increases sensitivity and the stability of reaction.
Detect in liquid and utilize POSSFF to improve the detectability detecting liquid, POSSFF (Blue ?fluorescentconjugated ?oligomersubstitutedpolyhedraloligomericsilsesquioxane) is that Cong dimer is the arm star compound of arm with POSS (Polyhedral Oligomeric sesquialter silane) for core.
POSSFF has very strong fluorescence in aqueous, its fluorescence quantum yield is about 0.85, can as signal amplifier, by FRET (FRET) effect, RNA and DNA is distinguished, the Fluorescent signal of dsDNA or ssRNA being marked with fluorophor can be amplified 52 times.Its advantage is the generation being conducive to colleges and universities FRET process, thus improves detection sensitivity; Secondly, the water-soluble of amino group Graphene is strengthened.Comprise the ssDNA probe that is marked with fluorescence molecule and can with the liquid conjugated compound amino group Graphene of ssDNA probe generation FRET; Detection liquid and detected liquid inject micro-fluidic chip by automatic injection pump, through hatching, carrying out fluoroscopic examination, thus carrying out qualitative and quantitative analysis to the biological heredity species in detected liquid.
The present invention has prepared the detection liquid of 6 samples, has formula as shown in table 1 below:
Table 1
Its preparation method is: under lucifuge condition, amination graphene oxide is mixed with fluorescence probe equal because of, jointly hatch 3min, (add malic acid again and hatch 5min), so obtained transducer detected liquid.
Described DNA molecular probe is:
Multi-Objective Genetic fragment (16s or 18sDNA) Auele Specific Primer synthesizes dsDNA by gene clone technology, dsDNA fragment, with sybergreen non-covalent labeling phycoerythrin (PE).
Embodiment 3 is based on the testing process of micro-fluidic chip to gene:
Detection liquid is simultaneously joined micro-fluidic chip sample introduction zone from different injection port by miniflow pump from sample liquid; Two kinds of solution are mixed fully by micro-mixer, detect after hatching 20min; After detection, waste liquid is discharged by outlet, completes whole testing process.Utilize fluorescence microscope CCD lighting apparatus to carry out collection and the record of fluorescence signal, after image processing software ImageJ process, obtain the luminous intensity quantitative data of fluorescence.
Carry out conventional saliva, sweat, blood testing by described system, its detectable limit is as shown in table 2 below:
Table 2
| Saliva | Sweat | Blood | |
| Sample 1 | 8 | 10 | 10 |
| Sample 3 | 10 | 11 | 9 |
| Sample 4 | 3 | 2 | 5 |
| Sample 5 | 9 | 9 | 11 |
| Sample 6 | 9 | 10 | 10 |
Encryption mechanism more complicated of the present invention, realizes random sequence by hardware, therefore has the fail safe of height.
The present invention first noise sequence and the 3rd noise sequence all come from hardware interface, and the second noise sequence comes from memory, substantially do not need computing to generate, so very save operational capability, make encrypting and decrypting have arithmetic speed soon.
Data transaction is become view data to process by the present invention, in proof procedure, only require that contrast coincidence rate exceedes preset value, it is very accurate that this makes the transfer of data between key end and transformer substation end not require, noise sequence may not be very accurate, thus allow certain error code, This further reduces hardware cost, and be suitable for the very complicated place of wireless environment.
In sum, the technical scheme that the embodiment of the application provides have employed hardware mechanisms to obtain random number, have employed image mechanism to be encrypted, thus existing very high fail safe, there is again very fast arithmetic speed, and certain error code can also be allowed.
Those skilled in the art, at consideration specification and after putting into practice invention disclosed herein, will easily expect other embodiment of the present invention.The application is intended to contain any modification of the present invention, purposes or adaptations, and these modification, purposes or adaptations are followed general principle of the present invention and comprised the undocumented common practise in the art of the application or conventional techniques means.Specification and embodiment are only regarded as exemplary, and true scope of the present invention and spirit are pointed out by claim below.
Should be understood that, the present invention is not limited to precision architecture described above and illustrated in the accompanying drawings, and can carry out various amendment and change not departing from its scope.Scope of the present invention is only limited by appended claim.
Claims (1)
1. a unattended operation transformer station, it comprises transformer substation end and key end, is characterised in that, transformer substation end comprises demo plant, decryption device and transformer substation end wireless device, and key end comprises encryption device and key end wireless device;
Encryption device comprises:
Micro-fluidic chip detection module, for obtaining initial data string A
0={ a
n, wherein, a is initial data string A
0in character, n is the figure place of a;
Micro-fluidic chip detection module is a kind of DNA detection module, comprising: transducing signal detection zone, transducing signal mixed zone and transducing signal output area.
Transducing signal detection zone is mainly used to obtain the primary sample of user, and transducing signal mixed zone is mainly used in nano-sensor and detects and fully the mixing of primary sample, and enters into transducing signal detection zone after mixing is complete; Transducing signal detection zone is mainly used in the detection of the fluorescence signal produced after nano-sensor detects target gene; Detect complete data and obtain initial data string A
0,
Described micro-fluidic chip, is characterized in that, overall dimensions is: wide 30mm, high 20mm, dark 90 μm, the wide 24mm in structural core district, high 16mm; Micro-fluidic chip forms by 4 layers, ground floor substrate: glass, second and third layer: ultra-violet curing glue, 4th layer: mask, each interlayer adhesive: NOA81 optical cement, adopt plasma oxidation bonding method to make substrate and the sealing-in of ground floor structure, the chip structure of each layer is made up of photoetching process;
Matrixing module, for by initial data string A
0matrixing obtains matrix A={ a
ijah × aw, specifically comprise: to { a
ni-1 character is got successively from head of the queue, when odd-times gets character, mend 10 at the string head of the character string obtained, supply as a line a
i, when even-times gets character, mend 11 at the string tail of the character string obtained, supply as a line a
i, for the character string obtained for the last time, then first open from string and begin to mend 10 every a character, until supply as a line a
i, by all a obtained
imatrix A={ a is obtained according to the pseudo-random sequence arrangement preset
ijah × aw, wherein, ah is the height of matrix A, and aw is the height of matrix A;
First noise module, the last position of the amplitude that the shortwave recording key end wireless device monitors predeterminated frequency in real time obtains forms the first noise data sequence C T1 by odd number fetch bit;
First scrambling module, for from first element of matrix A, gets the first random function R (1) individual element successively and is inserted in matrix A and obtains matrix B={ b from the first noise data sequence C T1
stbh × bw,
Wherein, bh is the height of matrix B, and bw is the height of matrix B, first random function R (1)=CT1
s%64, CT1
sfor the number obtained successively from the first noise data sequence C T1;
The figure place of noise data makes s be 32 times of i, and t is 32 times of j;
3rd noise module, the last position of the amplitude that the shortwave recording key end wireless device monitors predeterminated frequency in real time obtains forms the 3rd noise data sequence C T3 by even number fetch bit;
3rd scrambling module, for matrix B and the 3rd noise data sequence C T3 are carried out XOR, obtains enciphered data: Matrix C={ c
stch × cw, ch be the height of Matrix C, cw is the height of Matrix C;
Transformer substation end wireless device and key end wireless device all for monitoring the wireless short wave of identical predeterminated frequency, to obtain the first noise sequence CT1 and the 3rd noise sequence CT3;
Decryption device is used for the noise data from transformer substation end wireless device with from the second noise sequence CT2 that transformer substation end prestores, and the enciphered data from encryption device is performed to the inverse operation of the cryptographic calculation of above-mentioned encryption device;
Demo plant is used for decryption device to compare to the initial data that the enciphered data from encryption device carries out data that inverse operation obtains and transformer substation end, if comparison coincidence rate exceedes preset value, then confirms as and is verified.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106154885A (en) * | 2016-08-26 | 2016-11-23 | 张振堂 | A kind of intelligent substation security protection system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106154885A (en) * | 2016-08-26 | 2016-11-23 | 张振堂 | A kind of intelligent substation security protection system |
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