CN105301869A - Cell plate clone formation image acquisition instrument - Google Patents
Cell plate clone formation image acquisition instrument Download PDFInfo
- Publication number
- CN105301869A CN105301869A CN201510884035.0A CN201510884035A CN105301869A CN 105301869 A CN105301869 A CN 105301869A CN 201510884035 A CN201510884035 A CN 201510884035A CN 105301869 A CN105301869 A CN 105301869A
- Authority
- CN
- China
- Prior art keywords
- image capture
- clone formation
- formation image
- cell
- cell plates
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03B—APPARATUS OR ARRANGEMENTS FOR TAKING PHOTOGRAPHS OR FOR PROJECTING OR VIEWING THEM; APPARATUS OR ARRANGEMENTS EMPLOYING ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ACCESSORIES THEREFOR
- G03B15/00—Special procedures for taking photographs; Apparatus therefor
- G03B15/02—Illuminating scene
- G03B15/03—Combinations of cameras with lighting apparatus; Flash units
- G03B15/05—Combinations of cameras with electronic flash apparatus; Electronic flash units
-
- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03B—APPARATUS OR ARRANGEMENTS FOR TAKING PHOTOGRAPHS OR FOR PROJECTING OR VIEWING THEM; APPARATUS OR ARRANGEMENTS EMPLOYING ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ACCESSORIES THEREFOR
- G03B15/00—Special procedures for taking photographs; Apparatus therefor
- G03B15/02—Illuminating scene
- G03B15/06—Special arrangements of screening, diffusing, or reflecting devices, e.g. in studio
Landscapes
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to an image acquisition instrument with lighting and image acquisition functions, and specifically relates to an image acquisition instrument for image acquisition after a cell plate clone experiment for research of cytobiological function and tumor cell function. The image acquisition instrument mainly solves the problems that if only a digital camera is used by manpower to take pictures after cell clone formation, the image acquisition angle is unsuitable, and the image quality is low and the result analysis is influenced. The cell plate clone formation image acquisition instrument includes an acquisition house, wherein one part of the bottom and one part of the side wall of the acquisition house are open-mouthed structures; a light panel is arranged at the bottom of the acquisition house; a plurality of lights are arranged above the light panel; a light source backboard is arranged above the lights and at the middle lower part of the acquisition house; a lens hole is formed in a top board of the acquisition house; and a sample placing plate is movably arranged on the light source backboard.
Description
Technical field
The present invention is the image capture instrument with illumination and image collecting function, is specifically related to the image collecting device after gathering cell plates cloning experimentation in the research such as cell biological function, tumour cell function.
Background technology
Cell plates cloning experimentation is adherent surviving and form the quantity of cloning after the inoculation of reflection cell.Cell after adherent is not necessarily each can breed and be formed clone, and the cell forming clone must be the adherent cell with there being proliferation activity.Clone formation then can reflect cell colony dependence and multiplication capacity two important characters, is assessment cell, the especially Classic Experiments of the pernicious biological characteristics of tumour cell.But, the instrument that after plate clone, the collection of image does not adapt, classic method only uses digital camera artificial lighting, and the method exists following shortcoming: 1, shooting process is artificial, and often image can not level obtain; 2, shooting process needs suitable light, can keep the sun off or the irradiation of daylight lamp, cause shooting shade, effect diagram tablet quality during shooting; 3, adopt artificial light or camera flash lantern festival to bring the reflective of dull and stereotyped ware, also affect picture quality, have a strong impact on the interpretation of result after picked-up picture.
Summary of the invention
The present invention mainly for artificial simple using on the mode of taking a picture after cell clonal formation, digital camera exists that image capturing angle is improper, poor image quality and affect the problem of interpretation of result, a kind of cell plates Clone formation image capture instrument is provided.
The present invention is the technical scheme taked that solves the problem:
A kind of cell plates Clone formation image capture instrument, comprise and gather storehouse, wherein: described collection storehouse is a part for bottom and sidewall is uncovered structure, a tabula rasa is provided with in the bottom gathering storehouse, multiple lamp is provided with above tabula rasa, be positioned at above lamp in the middle and lower part gathering storehouse and be provided with light source backboard, the top board gathering storehouse offers camera aperture, light source backboard is movably equipped with sample and places plate.
Further, described collection storehouse is anterior and bottom is uncovered cube structure, and four columns gathering storehouse are Telescopic rod structure.
A LED light band is respectively had above the sidewall of the left and right sides gathering storehouse.
Collection storehouse inwall posts reflective sheeting.
Plate placed by described light source backboard and sample is all white printing opacity acrylic board, and described sample placement plate is two, and a sample is placed on plate and evenly offered multiple double dish groove, and another sample is placed on plate and offered cell board slot.
The cold light source LED of described lamp to be colour temperature be natural light, and multiple lamp arranges in " returning " shape.
The cold light source LED of described light belt to be colour temperature be natural light.
The circular port of described camera aperture to be diameter be 10cm.
The circular groove of described double dish groove to be diameter be 40mm, described cell board slot for long be 13cm, wide is the rectangular slot of 9cm.
Described reflective sheeting is frosted tinfoil.
The present invention adopts technique scheme, arranges the LED of " returning " shape arrangement in the bottom gathering storehouse, and respectively has a light belt above the sidewall of the left and right sides gathering storehouse, ensures uniform light when taking a picture; Described in employing as frosted tinfoil reflective sheeting, ensure that the soft reflections of light; Two samples are placed plate and can be selected according to actual conditions; Diameter is that the circular lens hole of 10cm compatiblely can count slr camera diameter of lens absolutely greatly, ensures that camera is stable, vertical hanging is placed, is beneficial to shooting image; Four is the height in the adjustable collection storehouse of column of Telescopic rod structure, reaches the object of adjusting focal length.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention;
Fig. 2 is the structural representation that inventive samples places plate.
Embodiment
Embodiment 1
Cell clonal formation is tested:
With the cell of collected by trypsinisation difference group after cell transfecting or medication 48h, count with 1mL nutrient culture media re-suspended cell.Blow and beat into single cell suspension, cell count, cell dilution is become 1 × 10
4individual/milliliter, then be diluted to 1 × 10
3individual/milliliter is inoculated in porous plate or double dish, inhales 50uL inoculation and 50cell/ hole, inhales 100uL inoculation and 100cell/ hole, inhales 200uL inoculation and 200cell/ hole.Inoculate complete, each hole supplemented medium is to 300uL/ hole.Horizontal position left-right and front-back rocks double dish or porous plate, makes hole inner cell be evenly distributed as far as possible.Cultivate after 7 days, suck each hole nutrient culture media, PBS or physiological saline rinsing 2 times, each hole adds 200uL violet staining liquid fully at the bottom of coverage hole, should be placed in PBS wash buffer by porous plate or double dish plate after 20min, notices that current are wanted slowly, slightly dry after flushing, treat image acquisition and analysis of accounts.
Embodiment 2
A kind of cell plates Clone formation image capture instrument, as depicted in figs. 1 and 2, comprise and gather storehouse 1, wherein: described collection storehouse 1 is anterior and bottom is uncovered cube structure, and four columns 7 gathering storehouse 1 are Telescopic rod structure, a tabula rasa 2 is provided with in the bottom gathering storehouse 1, the cold light source LED 3 that colour temperature is natural light is provided with above tabula rasa 2, and the arrangement in " returning " shape of multiple lamp 3, the light source backboard 4 being provided with above lamp 3 and being made up of white printing opacity acrylic board is positioned in the middle and lower part gathering storehouse 1, the top board gathering storehouse 1 offers camera aperture 5, the circular port of camera aperture 5 to be diameters be 10cm, light source backboard 4 is movably equipped with the sample be made up of white printing opacity acrylic board and places plate 6, and sample placement plate 6 is two, a sample is placed on plate 6 and is offered two parallel row's double dish grooves 8, often arrange 3, be total to consistency from top to bottom and equally spaced two rows, another sample is placed on plate 6 and is offered cell board slot 9, above the sidewall of the left and right sides gathering storehouse 1, respectively there is a colour temperature to be the cold light source LED 10 of natural light, collection storehouse 1 inwall posts the reflective sheeting 11 that frosted tinfoil is made, described double dish groove 8 for diameter be the circular groove of 40mm, described cell board slot 9 is long is 13cm, wide is the rectangular slot of 9cm.
Corresponding sample is selected to place plate 6 according to the double dish after cell clonal formation or porous culture plate, sample is placed plate 6 and is positioned on light source backboard 4, then put double dish or porous plate successively, open LED 3 and LED 10, the height of four columns 7 is adjusted according to actual conditions, digital camera lens is stretched into the camera aperture 5 on top board, adjusting focal length carries out image capture, then analyzes.
Claims (10)
1. a cell plates Clone formation image capture instrument, comprise and gather storehouse (1), it is characterized in that: described collection storehouse (1) for a part for bottom and sidewall be uncovered structure, a tabula rasa (2) is provided with in the bottom gathering storehouse (1), multiple lamp (3) is provided with in the top of tabula rasa (2), the top being positioned at lamp (3) in the middle and lower part gathering storehouse (1) is provided with light source backboard (4), the top board gathering storehouse (1) offers camera aperture (5), light source backboard (4) is movably equipped with sample and places plate (6).
2. cell plates Clone formation image capture instrument according to claim 1, it is characterized in that: described collection storehouse (1) is anterior and bottom is uncovered cube structure, and four columns (7) gathering storehouse (1) are Telescopic rod structure.
3. according to the cell plates Clone formation image capture instrument in claim 1 or 2 described in any one, it is characterized in that: above the sidewall of the left and right sides gathering storehouse (1), respectively have a light belt (10).
4. according to the cell plates Clone formation image capture instrument in claim 1 or 2 described in any one, it is characterized in that: on collection storehouse (1) inwall, post reflective sheeting (11).
5. cell plates Clone formation image capture instrument according to claim 1, it is characterized in that: plate (6) placed by described light source backboard (4) and sample is all white printing opacity acrylic board, and described sample placement plate (6) is two, a sample is placed on plate (6) and is evenly offered multiple double dish groove (8), and another sample is placed on plate (6) and offered cell board slot (9).
6. cell plates Clone formation image capture instrument according to claim 1, is characterized in that: described lamp (3) for colour temperature be the cold light source LED of natural light, and multiple lamp (3) in " returning " shape arrange.
7. cell plates Clone formation image capture instrument according to claim 3, is characterized in that: described light belt (10) for colour temperature be the cold light source LED of natural light.
8. cell plates Clone formation image capture instrument according to claim 1, is characterized in that: described camera aperture (5) for diameter be the circular port of 10cm.
9. cell plates Clone formation image capture instrument according to claim 5, is characterized in that: described double dish groove (8) for diameter be the circular groove of 40mm, described cell board slot (9) for long be 13cm, wide is the rectangular slot of 9cm.
10. cell plates Clone formation image capture instrument according to claim 4, is characterized in that: described reflective sheeting (7) is frosted tinfoil.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510884035.0A CN105301869A (en) | 2015-12-04 | 2015-12-04 | Cell plate clone formation image acquisition instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510884035.0A CN105301869A (en) | 2015-12-04 | 2015-12-04 | Cell plate clone formation image acquisition instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105301869A true CN105301869A (en) | 2016-02-03 |
Family
ID=55199302
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510884035.0A Pending CN105301869A (en) | 2015-12-04 | 2015-12-04 | Cell plate clone formation image acquisition instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105301869A (en) |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0625569A1 (en) * | 1992-08-27 | 1994-11-23 | Iul, S.A. | Contrast chamber for spotlighting bacterial colonies with respect to the culture medium thereof |
| CN2724084Y (en) * | 2004-09-02 | 2005-09-07 | 郭振和 | Color data read-in device for computer microbial biochemical reaction |
| CN201221714Y (en) * | 2008-06-30 | 2009-04-15 | 瑞安市博业激光应用技术有限公司 | Backlight source of CCD camera in laser equipment |
| CN201233492Y (en) * | 2008-07-07 | 2009-05-06 | 林璧光 | Telescopic image taking box for portable articles |
| JP2012529025A (en) * | 2009-06-02 | 2012-11-15 | コミッサリアータ レネルジー アトミック エ オゼネルジー アルテルナティーブ | Microlens imaging system and sample detection system attached device |
| CN103502422A (en) * | 2011-03-04 | 2014-01-08 | Lbt创新有限公司 | Image capture and lighting apparatus |
| CN203419930U (en) * | 2013-09-09 | 2014-02-05 | 大庆麦伯康生物技术有限公司 | Video recording light wave and illumination-adjustable incubator |
| CN203552653U (en) * | 2013-11-12 | 2014-04-16 | 郑州航空工业管理学院 | Animation showing device |
| CN103775951A (en) * | 2012-10-19 | 2014-05-07 | 上海铭源数康生物芯片有限公司 | Light-emitting diode (LED) array based standard plane light source |
| CN104542495A (en) * | 2014-07-01 | 2015-04-29 | 上海交通大学医学院附属新华医院 | Novel caenorhabditis elegans culture and real-time dynamic observation device |
| CN104633521A (en) * | 2013-11-06 | 2015-05-20 | 富泰华精密电子（郑州）有限公司 | Backlight module |
| CN205121130U (en) * | 2015-12-04 | 2016-03-30 | 山西医科大学第一医院 | Dull and stereotyped clone form images of cell gathers appearance |
-
2015
- 2015-12-04 CN CN201510884035.0A patent/CN105301869A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0625569A1 (en) * | 1992-08-27 | 1994-11-23 | Iul, S.A. | Contrast chamber for spotlighting bacterial colonies with respect to the culture medium thereof |
| CN2724084Y (en) * | 2004-09-02 | 2005-09-07 | 郭振和 | Color data read-in device for computer microbial biochemical reaction |
| CN201221714Y (en) * | 2008-06-30 | 2009-04-15 | 瑞安市博业激光应用技术有限公司 | Backlight source of CCD camera in laser equipment |
| CN201233492Y (en) * | 2008-07-07 | 2009-05-06 | 林璧光 | Telescopic image taking box for portable articles |
| JP2012529025A (en) * | 2009-06-02 | 2012-11-15 | コミッサリアータ レネルジー アトミック エ オゼネルジー アルテルナティーブ | Microlens imaging system and sample detection system attached device |
| CN103502422A (en) * | 2011-03-04 | 2014-01-08 | Lbt创新有限公司 | Image capture and lighting apparatus |
| CN103775951A (en) * | 2012-10-19 | 2014-05-07 | 上海铭源数康生物芯片有限公司 | Light-emitting diode (LED) array based standard plane light source |
| CN203419930U (en) * | 2013-09-09 | 2014-02-05 | 大庆麦伯康生物技术有限公司 | Video recording light wave and illumination-adjustable incubator |
| CN104633521A (en) * | 2013-11-06 | 2015-05-20 | 富泰华精密电子（郑州）有限公司 | Backlight module |
| CN203552653U (en) * | 2013-11-12 | 2014-04-16 | 郑州航空工业管理学院 | Animation showing device |
| CN104542495A (en) * | 2014-07-01 | 2015-04-29 | 上海交通大学医学院附属新华医院 | Novel caenorhabditis elegans culture and real-time dynamic observation device |
| CN205121130U (en) * | 2015-12-04 | 2016-03-30 | 山西医科大学第一医院 | Dull and stereotyped clone form images of cell gathers appearance |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107680129B (en) | Portable bacterial colony automatic counting method based on smart phone | |
| CN208126078U (en) | A kind of fruit is taken pictures acquisition device | |
| ES2745507T3 (en) | Lighting apparatus and image capture | |
| CN107828654A (en) | Based on the cytoactive no-mark monitoring apparatus and method being imaged without Lenses Diffractive | |
| CN202330966U (en) | Shadowless diffuse-light image acquisition device | |
| CN209330687U (en) | A kind of small plant root culture and detection device | |
| CN201708860U (en) | Outdoor plant image collecting device | |
| CN218847950U (en) | A phenotype image collection system for seed culture and germination period | |
| CN112595367A (en) | Rice root system property nondestructive measurement device based on intelligent robot | |
| CN205121130U (en) | Dull and stereotyped clone form images of cell gathers appearance | |
| CN105301869A (en) | Cell plate clone formation image acquisition instrument | |
| US10815313B2 (en) | Method for preparing a cellulose sponge | |
| CN105861302A (en) | Uniform lighting device for alga culture based on aquatic organism ecotoxicity evaluation | |
| CN209784139U (en) | Flow type counting system for scanning and focusing plankton | |
| CN113960044A (en) | Visual acquisition equipment for jade processing degree inspection and use method thereof | |
| CN209215836U (en) | A kind of multi-functional observing system of zebra fish culture | |
| CN206791297U (en) | A kind of device of the growth of potato test tube seedling induced with laser and its monitoring | |
| CN203259474U (en) | System for automatically measuring adhesiveness between asphalt and aggregate | |
| CN202631840U (en) | Small inverted biological microscope | |
| CN216748407U (en) | Device for measuring leech shape | |
| CN206244813U (en) | A kind of cell culture tube and Cell culture rack | |
| CN205682217U (en) | A kind of diamondback moth imago breeding device | |
| CN205408745U (en) | Make more even flower planting light filling device of flowers photic | |
| CN208801797U (en) | A kind of illuminated three-dimensional peasant picture art work | |
| CN218675616U (en) | Infrared lamp combined arrangement structure |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160203 |
|
| RJ01 | Rejection of invention patent application after publication |