CN105300932A - Surface plasma resonance rapid detection method of dibutyl phthalate - Google Patents
Surface plasma resonance rapid detection method of dibutyl phthalate Download PDFInfo
- Publication number
- CN105300932A CN105300932A CN201510778754.4A CN201510778754A CN105300932A CN 105300932 A CN105300932 A CN 105300932A CN 201510778754 A CN201510778754 A CN 201510778754A CN 105300932 A CN105300932 A CN 105300932A
- Authority
- CN
- China
- Prior art keywords
- dbp
- solution
- chip
- golden
- detection method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention provides a surface plasma resonance (SPR) rapid detection method of dibutyl phthalate (DBP). According to the surface plasma resonance rapid detection method, gold film surfaces of gold chips are loaded with DBP monoclonal antibodies via modification; the modified gold chips are delivered into a SPR detector; flowing of a bovine serum albumin DBP-BSA mixed liquid of the target compound DBP and high molecular competition compound DBP through the gold films as a mobile phase is carried out, so that competitive binding of the target compound DBP and the high molecular competition compound DBP-BSA with antibodies on the surfaces of the gold films is realized; a SPR detector is used for measuring gold chip surface light length change; and when the concentration of the high molecular competition compound DBP-BSA is constant, the concentration of the target compound DBP with the signals detected by the SPR detector are inversely associated, so that indirect measuring of the DBP content on the surfaces of the gold chips can be realized. The surface plasma resonance rapid detection method is high in sensitivity, accurate in detection results, short in detection time, and high in working efficiency.
Description
Technical field
The invention belongs to light textile consumer goods poisonous and harmful residues detection field, relating to and adopt surface plasma body resonant vibration (SPR) technology, particularly relating to a kind of surface plasma resonance rapid detection method that a kind of surplus by detecting butyl phthalate (DBP) antibody in sample extraction liquid detects DBP content in extract.
Background technology
Butyl phthalate (DBP) is that one uses phthalic ester plasticizer widely.In the light textile such as textile garment and the toy consumer goods, phthalic ester plasticizer is mainly used in, in Polyvinylchloride (PVC), leatheret, coating, ink jet printing and hot pressing applique product, can effectively reducing dyestuff viscosity, improves coating elasticity.Phthalic ester also can be used as composite component in textile auxiliary, because it is the non-ionic compound containing ester and benzene ring structure, so close dacron, thus level dyeing effect when being reached disperse dyeing by slow dye, it is one of component of levelling agent, also can be used as dyeing carrier, there is plasticization.
" mist generating agent " event with the outburst of TaiWan, China food service industry of constantly recalling of the outlet consumer goods (textile garment and toy), again brings phthalic ester into the popular visual field, causes the huge concern of society.Scientific research shows that phthalate (PAEs) is a kind of environment incretion interferent, has estrogen effect, is combined after it enters human body with corresponding hormone receptor, interfering bodies internal hormone normal level, thus affects reproduction, growth.Particularly serious on the impact of male children.In order to reduce the harm risk of PAEs to human body, the countries and regions such as European Union, the U.S., Canada, Brazil have put into effect relevant laws regulation all, have made clear and definite limitation requirement to its use.At present, phthalic ester is comprised to the research of the detection method of DBP both at home and abroad, mainly utilize gas chromatography, gas chromatograph-mass spectrometer, liquid chromatography, liquid chromatography-mass spectrography series connection etc. as analysis means.Therefore, set up SPR detection method that is sensitive, DBP efficiently, have important practice significance for the new opplication of exploration SPR technique in consumer goods residues detection.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of surface plasma resonance rapid detection method of highly sensitive, detection time is short, work efficiency is high butyl phthalate.
In order to solve the problems of the technologies described above, technical scheme of the present invention is to provide a kind of surface plasma resonance rapid detection method of butyl phthalate, it is characterized in that: butyl phthalate DBP monoclonal antibody in the golden film finishing of golden chip, golden chip after modifying is placed in surface plasma body resonant vibration SPR detector, make the mixed liquor of the bovine serum albumin(BSA) DBP-BSA of object DBP and macromolecule competitor DBP flow through golden film with mobile phase, then object DBP and macromolecule competitor DBP-BSA competition binding are on the antibody on golden film surface; The long change of golden chip surface light is detected by SPR detector; When the timing of macromolecule competitor DBP-BSA concentration one, object DBP concentration is lower, and the signal that SPR detector detects is higher; Object DBP concentration is higher, and the signal that SPR detector detects is lower; Therefore, the signal that object DBP concentration and SPR detector detect is inverse relation, thus indirect determination gold chip surface DBP content.
Preferably, concrete steps are as follows:
Step 1: the modification of sensing gold chip
1.1 cleanings: by ammoniacal liquor, hydrogen peroxide and deionized water wiring solution-forming, heating water bath emits bubble to solution, place golden chip and soak in this solution, use deionized water rinsing immediately, then dry up golden chip surface with nitrogen after then taking out;
1.2 self assembly bifunctional molecules: cleaned golden chip 11-Mercaptoundecanoic acid ethanolic solution submergence, soaked overnight; By the covalently bound self assembly bifunctional molecule of Au-S key; The golden chip aqua sterilisa of soaked overnight is cleaned, uses N
2dry up;
1.3 bifunctional molecule activation: first drip EDC solution at golden chip surface, then drip NHS solution, room temperature leaves standstill;
1.4 modify monoclonal antibody: golden chip washing dried up, drip DBP monoclonal antibody solution, be placed in constant temperature oven at golden chip surface;
1.5 close avtive spots: golden chip is taken out and wash dry up, and at golden chip surface dropping ethanolamine hydrochloric salt solution, room temperature is standing; Golden chip is cleaned and dries up for subsequent use, or be positioned in PBS solution and store;
Step 2:DBP standard model measures
2.1 baseline balances: the golden chip of modified is put into surface plasma body resonant vibration SPR detector, and conditioning instrumentation temperature, passes into PBS solution, regulates flow velocity, starts experiment after the signal stabilization that SPR detector detects; Experiment is carried out under Condition with fixed angle;
The determination of 2.2 competitor concentration: load competitor standard solution by quantitative loop, after the attached balance of adsorption/desorption to be achieved, pass into PBS solution and clean golden chip, wash away non-specific adsorption sample, carry out object analysis by the amount of specific adsorption sample; With resonance response units of delta RU value for ordinate, with the competitor concentration of standard solution added for horizontal ordinate curve plotting, the concentration that curve reaches balance is suitable competitor concentration;
Wherein, competitor standard solution is: take a certain amount of DBP-BSA, adds PBS solution constant volume, and the concentration configuring serial DBP-BSA standard solution is: 5 ~ 70ppm;
The foundation of 2.3 typical curves: by quantitative loop load sample standard solution, after the attached balance of adsorption/desorption to be achieved, passes into PBS solution and cleans golden chip; With resonance response units of delta RU value for ordinate, with the concentration of DBP in the sample standard solution added for horizontal ordinate curve plotting;
Wherein sample standard solution is the DBP mixed solution (x=5n, n ∈ [0,9]) of DBP-BSA and the xppm of competitor concentration suitable described in step 2.2;
2.4 sample tests: according to calibration curve method test sample standard solution, according to its Δ RU value, typical curve reads DBP content;
2.5 chip regeneration: detected sample standard solution, passed into glycine-HCI regenerated liquid and carry out golden chip regeneration; Finally, pass into PBS solution and clean golden chip.
Preferably, in described step 1.2,11-Mercaptoundecanoic acid ethanol solution concentration is 1mmol/L, and temperature is 4 DEG C.
Preferably, in described step 1.3, EDC solution and NHS solution are 150 μ L; Room temperature time of repose is 1h.
Preferably, in described step 1.4, DBP monoclonal antibody solution is 200 μ L; Be placed in 37 DEG C of constant temperature oven 3h.
Preferably, in described step 1.5, ethanolamine hydrochloric salt solution is 200 μ L, 1mol/L, and room temperature time of repose is 1h; PBS solution is 4 DEG C.
Preferably, in described step 2.1, conditioning instrumentation temperature makes it lower than room temperature 1 ~ 2 DEG C, regulates PBS solution flow velocity to be 30 μ L/min.
Preferably, in described step 2.5, the glycine-HCI regenerated liquid 6min passing into pH=2.2 carries out chip regeneration.
Preferably, described DBP-BSA is DBP coupling protein;
Described EDC solution is 1-(3-the dimethylamino-propyl)-3-ethyl carbodiimide salt solusion of 0.4mol/L;
Described NHS solution is the N-hydroxy-succinamide solution of 0.1mol/L;
Described PBS solution is the phosphate buffer of pH=7.4.
Compared to existing technology, the present invention has following beneficial effect:
1, adopt indirect SPR detection method to measure the content of DBP, by modifying DBP monoclonal antibody, detecting the concentration that the content remaining DBP-BSA macromolecule competitor in sample solution carrys out indirect determination DBP, effectively improve sensitivity; Detection time is short, and can detect result in general 15min, amount of samples is few;
2, by SPR instrument record, decrease the artificial subjectivity judged, chip can realize batch and modify, and work efficiency is high.
Embodiment
For making the present invention become apparent, hereby with a preferred embodiment, be described in detail below.
The invention provides a kind of surface plasma resonance rapid detection method of butyl phthalate, utilize the SPR detector with surface plasma resonance sensor, to detect the change of fixed angles light intensity, the content of DBP in indirect determination sample.Main technical schemes is: DBP monoclonal antibody (DBP-6C6) in golden film finishing, and the mobile phase flowing through golden film is the mixed liquor of the bovine serum albumin(BSA) (DBP-BSA) of object DBP and macromolecule competitor DBP.Object DBP and the macromolecule competitor DBP-BSA competition binding added are on the antibody of chip surface, and when the timing of macromolecule competitor DBP-BSA concentration one, object DBP concentration is lower, and signal is higher; DBP concentration is higher, and signal is lower.Therefore, object DBP concentration and signal are certain inverse relation.DBP-BSA not combined in solution is detected by SPR detector, thus DBP content in indirect determination testing sample.Concrete operation step is as follows:
Step 1: the modification of sensing chip
1.1 cleanings: the ammoniacal liquor (NH of 1 part 30%
4hydrogen peroxide (the H of OH) and 1 part 30%
2o
2), add the deionized water of 5 parts, heating water bath slightly emits bubble to solution, now temperature about 80 ~ 90 DEG C.Golden chip is placed and soaks 10 minutes in this solution, use deionized water rinsing immediately after taking-up, then dry up chip surface with nitrogen, and start experiment as early as possible.When use brand-new golden chip, can be reduced to: with deionized water, chip surface is cleaned, after dry up with nitrogen again.
1.2 self assembly bifunctional molecules: 11-Mercaptoundecanoic acid (MUA) the ethanolic solution submergence of cleaned gold plaque 1mmol/L, 4 DEG C of soaked overnight.By the covalently bound self assembly bifunctional molecule of Au-S key.The chip aqua sterilisa of soaked overnight is cleaned, dries up with N2.
1.3 bifunctional molecule activation: the EDC solution first dripping 150 μ L at chip surface, then the NHS solution dripping 150 μ L, room temperature leaves standstill 1h.
1.4 modify monoclonal antibody: chip washing dried up, drip DBP monoclonal antibody (DBP-6C6) solution of 200 μ L, be placed in 37 DEG C of constant temperature oven 3h at chip surface.
1.5 close avtive spot: chip washing dried up, drip the 1mol/L ethanolamine hydrochloric salt solution of 200 μ L at chip surface, room temperature leaves standstill 1h.Chip is cleaned and dries up for subsequent use, or be positioned in PBS solution, 4 DEG C of storages.
Step 2:DBP standard model measures
2.1 baseline balances: the chip of modified is put into SPR instrument, and conditioning instrumentation temperature makes, lower than room temperature 1 ~ 2 DEG C, to pass into PBS solution, regulates flow velocity to be 30 μ L/min, starts experiment after curve is stable.Experiment is carried out under Condition with fixed angle.
The determination of 2.2 competitor concentration: load competitor standard solution by quantitative loop, after the attached balance of adsorption/desorption to be achieved, passes into PBS solution cleaning chip, washes away non-specific adsorption sample, carry out order analysis by the amount of specific adsorption.With resonance response units of delta RU value for ordinate, with the competitor concentration of standard solution added for horizontal ordinate curve plotting, the concentration that curve reaches balance is suitable competitor concentration, as 40ppm.
Wherein competitor standard solution is: take a certain amount of DBP-BSA, adds PBS solution constant volume, and the concentration configuring serial DBP-BSA standard solution is: 5 ~ 70ppm.
The foundation of 2.3 typical curves: by quantitative loop load sample standard solution, after the attached balance of adsorption/desorption to be achieved, passes into PBS solution cleaning chip.With resonance response units of delta RU value for ordinate, with the concentration of DBP in the sample standard solution added for horizontal ordinate curve plotting.
Wherein sample standard solution is: the DBP mixed solution (x=5n, n ∈ [0,9]) of DBP-BSA and the xppm of 40ppm.
2.4 sample tests: according to calibration curve method test DBP sample solution, according to its Δ RU value, typical curve reads BPA content.
2.5 chip regeneration: the solution having detected sample, glycine-HCI regenerated liquid about the 6min passing into pH=2.2 carries out chip regeneration, and in order to prevent the damage of chip decorative layer, the recovery time is unsuitable long.Finally, PBS solution cleaning chip is passed into.
Described DBP-6C6 monoclonal antibody takes from the mouse source antibody of DBP.
Described DBP-BSA macromolecule competitor is DBP coupling protein.
Described EDC solution is 1-(3-the dimethylamino-propyl)-3-ethyl carbodiimide salt solusion of 0.4mol/L.
Described NHS solution is the N-hydroxy-succinamide solution of 0.1mol/L.
Described PBS solution is the phosphate buffer of pH=7.4.
Claims (10)
1. the surface plasma resonance rapid detection method of a butyl phthalate, it is characterized in that: butyl phthalate DBP monoclonal antibody in the golden film finishing of golden chip, golden chip after modifying is placed in surface plasma body resonant vibration SPR detector, make the mixed liquor of the bovine serum albumin(BSA) DBP-BSA of object DBP and macromolecule competitor DBP flow through golden film with mobile phase, then object DBP and macromolecule competitor DBP-BSA competition binding are on the antibody on golden film surface; The long change of golden chip surface light is detected by SPR detector; When the timing of macromolecule competitor DBP-BSA concentration one, the signal that object DBP concentration and SPR detector detect is inverse relation, thus indirect determination gold chip surface DBP content.
2. the surface plasma resonance rapid detection method of a kind of butyl phthalate as claimed in claim 1, is characterized in that: concrete steps are as follows:
Step 1: the modification of sensing gold chip
1.1 cleanings: by ammoniacal liquor, hydrogen peroxide and deionized water wiring solution-forming, heating water bath emits bubble to solution, place golden chip and soak in this solution, use deionized water rinsing immediately, then dry up golden chip surface with nitrogen after then taking out;
1.2 self assembly bifunctional molecules: cleaned golden chip 11-Mercaptoundecanoic acid ethanolic solution submergence, soaked overnight; By the covalently bound self assembly bifunctional molecule of Au-S key; The golden chip aqua sterilisa of soaked overnight is cleaned, uses N
2dry up;
1.3 bifunctional molecule activation: first drip EDC solution at golden chip surface, then drip NHS solution, room temperature leaves standstill;
1.4 modify monoclonal antibody: golden chip washing dried up, drip DBP monoclonal antibody solution, be placed in constant temperature oven at golden chip surface;
1.5 close avtive spots: golden chip is taken out and wash dry up, and at golden chip surface dropping ethanolamine hydrochloric salt solution, room temperature is standing; Golden chip is cleaned and dries up for subsequent use, or be positioned in PBS solution and store;
Step 2:DBP standard model measures
2.1 baseline balances: the golden chip of modified is put into surface plasma body resonant vibration SPR detector, and conditioning instrumentation temperature, passes into PBS solution, regulates flow velocity, starts experiment after the signal stabilization that SPR detector detects; Experiment is carried out under Condition with fixed angle;
The determination of 2.2 competitor concentration: load competitor standard solution by quantitative loop, after the attached balance of adsorption/desorption to be achieved, pass into PBS solution and clean golden chip, wash away non-specific adsorption sample, carry out object analysis by the amount of specific adsorption sample; With resonance response units of delta RU value for ordinate, with the competitor concentration of standard solution added for horizontal ordinate curve plotting, the concentration that curve reaches balance is suitable competitor concentration;
Wherein, competitor standard solution is: take a certain amount of DBP-BSA, adds PBS solution constant volume, and the concentration configuring serial DBP-BSA standard solution is: 5 ~ 70ppm;
The foundation of 2.3 typical curves: by quantitative loop load sample standard solution, after the attached balance of adsorption/desorption to be achieved, passes into PBS solution and cleans golden chip; With resonance response units of delta RU value for ordinate, with the concentration of DBP in the sample standard solution added for horizontal ordinate curve plotting;
Wherein sample standard solution is the DBP mixed solution (x=5n, n ∈ [0,9]) of DBP-BSA and the xppm of competitor concentration suitable described in step 2.2;
2.4 sample tests: according to calibration curve method test sample standard solution, according to its Δ RU value, typical curve reads DBP content;
2.5 chip regeneration: detected sample standard solution, passed into glycine-HCI regenerated liquid and carry out golden chip regeneration; Finally, pass into PBS solution and clean golden chip.
3. the surface plasma resonance rapid detection method of a kind of butyl phthalate as claimed in claim 2, is characterized in that: in described step 1.1, by the hydrogen peroxide of the ammoniacal liquor of 1 part 30% and 1 part 30%, adds the deionized water wiring solution-forming of 5 parts; When heating water bath to solution emits bubble, solution temperature is 80 ~ 90 DEG C; Gold chip is placed and is soaked 10 minutes in this solution;
When using brand-new golden chip, described step 1.1 can be reduced to: clean golden chip surface with deionized water, then dry up with nitrogen.
4. the surface plasma resonance rapid detection method of a kind of butyl phthalate as claimed in claim 2, it is characterized in that: in described step 1.2,11-Mercaptoundecanoic acid ethanol solution concentration is 1mmol/L, temperature is 4 DEG C.
5. the surface plasma resonance rapid detection method of a kind of butyl phthalate as claimed in claim 2, is characterized in that: in described step 1.3, EDC solution and NHS solution are 150 μ L; Room temperature time of repose is 1h.
6. the surface plasma resonance rapid detection method of a kind of butyl phthalate as claimed in claim 2, is characterized in that: in described step 1.4, and DBP monoclonal antibody solution is 200 μ L; Be placed in 37 DEG C of constant temperature oven 3h.
7. the surface plasma resonance rapid detection method of a kind of butyl phthalate as claimed in claim 2, is characterized in that: in described step 1.5, and ethanolamine hydrochloric salt solution is 200 μ L, 1mol/L, and room temperature time of repose is 1h; PBS solution is 4 DEG C.
8. the surface plasma resonance rapid detection method of a kind of butyl phthalate as claimed in claim 2, is characterized in that: in described step 2.1, and conditioning instrumentation temperature makes it lower than room temperature 1 ~ 2 DEG C, regulates PBS solution flow velocity to be 30 μ L/min.
9. the surface plasma resonance rapid detection method of a kind of butyl phthalate as claimed in claim 2, is characterized in that: in described step 2.5, and the glycine-HCI regenerated liquid 6min passing into pH=2.2 carries out chip regeneration.
10. the surface plasma resonance rapid detection method of a kind of butyl phthalate as claimed in claim 1 or 2, is characterized in that:
Described DBP-BSA is DBP coupling protein;
Described EDC solution is 1-(3-the dimethylamino-propyl)-3-ethyl carbodiimide salt solusion of 0.4mol/L;
Described NHS solution is the N-hydroxy-succinamide solution of 0.1mol/L;
Described PBS solution is the phosphate buffer of pH=7.4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510778754.4A CN105300932B (en) | 2015-11-13 | 2015-11-13 | A kind of surface plasma resonance rapid detection method of butyl phthalate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510778754.4A CN105300932B (en) | 2015-11-13 | 2015-11-13 | A kind of surface plasma resonance rapid detection method of butyl phthalate |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105300932A true CN105300932A (en) | 2016-02-03 |
| CN105300932B CN105300932B (en) | 2018-06-15 |
Family
ID=55198442
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510778754.4A Expired - Fee Related CN105300932B (en) | 2015-11-13 | 2015-11-13 | A kind of surface plasma resonance rapid detection method of butyl phthalate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105300932B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106568744A (en) * | 2016-10-08 | 2017-04-19 | 浙江警察学院 | Method for detecting cocaine based on SPR technology |
| CN106568743A (en) * | 2016-10-08 | 2017-04-19 | 浙江警察学院 | SPR technique-based ketamine (K powder) detection method |
| CN106896086A (en) * | 2016-10-08 | 2017-06-27 | 浙江警察学院 | A kind of morphine detection method based on SPR technique |
| CN107037008A (en) * | 2016-10-08 | 2017-08-11 | 浙江警察学院 | A kind of crystal methamphetamine based on SPR technique（Methamphetamine）Detection method |
| CN107478613A (en) * | 2017-08-02 | 2017-12-15 | 杭州晶百检测技术有限公司 | A kind of preparation method of a variety of drug testing chips based on SPR |
| CN108593601A (en) * | 2018-04-11 | 2018-09-28 | 珠海国际旅行卫生保健中心 | The SPRi biochip preparing methods of quick detection HIV-1 a kind of and its application |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101929956A (en) * | 2010-07-29 | 2010-12-29 | 浙江大学 | Surface plasmon resonance and bio-sensing-based water chip |
| CN103323429A (en) * | 2013-05-30 | 2013-09-25 | 中南大学 | Surface plasmon resonance (SPR) sensor chip for detection of melamine content of milk and its preparation method and detection method |
| CN103792211A (en) * | 2014-01-27 | 2014-05-14 | 暨南大学 | Surface plasma resonance biochip as well as preparation method and application thereof |
-
2015
- 2015-11-13 CN CN201510778754.4A patent/CN105300932B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101929956A (en) * | 2010-07-29 | 2010-12-29 | 浙江大学 | Surface plasmon resonance and bio-sensing-based water chip |
| CN103323429A (en) * | 2013-05-30 | 2013-09-25 | 中南大学 | Surface plasmon resonance (SPR) sensor chip for detection of melamine content of milk and its preparation method and detection method |
| CN103792211A (en) * | 2014-01-27 | 2014-05-14 | 暨南大学 | Surface plasma resonance biochip as well as preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 刘珊珊等: "《牛血清蛋白-三聚氰胺偶联物修饰的表面等离子体激元共振芯片检测三聚氰胺含量》", 《分析化学》 * |
| 康丹妮: "《鲤鱼雌激素受体的分离纯化及其与邻苯二甲酸酯的相互作用研究》", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106568744A (en) * | 2016-10-08 | 2017-04-19 | 浙江警察学院 | Method for detecting cocaine based on SPR technology |
| CN106568743A (en) * | 2016-10-08 | 2017-04-19 | 浙江警察学院 | SPR technique-based ketamine (K powder) detection method |
| CN106896086A (en) * | 2016-10-08 | 2017-06-27 | 浙江警察学院 | A kind of morphine detection method based on SPR technique |
| CN107037008A (en) * | 2016-10-08 | 2017-08-11 | 浙江警察学院 | A kind of crystal methamphetamine based on SPR technique（Methamphetamine）Detection method |
| CN107478613A (en) * | 2017-08-02 | 2017-12-15 | 杭州晶百检测技术有限公司 | A kind of preparation method of a variety of drug testing chips based on SPR |
| CN108593601A (en) * | 2018-04-11 | 2018-09-28 | 珠海国际旅行卫生保健中心 | The SPRi biochip preparing methods of quick detection HIV-1 a kind of and its application |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105300932B (en) | 2018-06-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105300932A (en) | Surface plasma resonance rapid detection method of dibutyl phthalate | |
| Uludag et al. | Lab-on-a-chip based biosensor for the real-time detection of aflatoxin | |
| Yu et al. | Molecularly imprinted polymeric microspheres for determination of bovine serum albumin based on flow injection chemiluminescence sensor | |
| IL170730A (en) | Method and arrangement for detecting harmful substances | |
| Xiong et al. | Molecularly imprinted on-line solid-phase extraction combined with flow-injection chemiluminescence for the determination of tetracycline | |
| CN106018347B (en) | A kind of surface plasmon resonance sensing chip and its preparation method and application | |
| US20100297671A1 (en) | Method and Device for the Quantitative Determination of Analytes in Liquid Samples | |
| CN104132934A (en) | Preparation for molecularly-imprinted electroluminescent paper chip for detecting multiple sample pesticide residues | |
| CN105277520B (en) | A kind of preparation and application of Ratio-type fluorescence the oxygen sensing film | |
| EP1649049A1 (en) | Improvements in sensor chips | |
| CN110095596A (en) | Chemiluminescence based on Fe-MOFs-fluorescence double-bang firecracker answers immunosensor | |
| CN106383110A (en) | OTA chemiluminiscence detecting method based on nano-gold label aptasensor | |
| CN110305106A (en) | Related substance of a kind of amber love song Ge Lieting and preparation method thereof, analysis method and application | |
| CN107192709A (en) | A kind of heavy metal nickel ion quick detection test paper and its detection method | |
| Yuan et al. | Sensitive determination of rose bengal in brown sugar by a molecularly imprinted solid-phase extraction monolithic capillary column coupled with capillary electrophoresis | |
| CN106442480B (en) | OTA chemical luminescence detection method based on HRP label aptamer sensor | |
| CN110779912B (en) | Biotin-avidin or streptavidin microsphere-free homogeneous chemiluminescence system | |
| CN106353494A (en) | Detection test strip for dibutyl phthalate and preparation method thereof | |
| CN105891159A (en) | Molecularly imprinted-SPR sensing method based on histamine rapid detection | |
| CN110940814A (en) | Test strip and kit for quantitatively detecting KL-6 | |
| CN103543180A (en) | Immune impedance biosensor detection method and device for quick pesticide residue detection | |
| CN106645055A (en) | Melamine testing strip and preparation method thereof | |
| CN109283165B (en) | Preparation method and application of fluorescent test strip for analyzing trace water in organic reagent | |
| CN101799457B (en) | Method for measuring betacyclodextrin content of fabrics | |
| CN113125421B (en) | Optical fiber biosensor and application thereof in homogeneous phase chemiluminescence biological detection |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180615 Termination date: 20211113 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |