CN105296562A - Glutamic acid fermentation culture medium using betaine phosphate to substitute betaine hydrochloride - Google Patents
Glutamic acid fermentation culture medium using betaine phosphate to substitute betaine hydrochloride Download PDFInfo
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- CN105296562A CN105296562A CN201510733729.4A CN201510733729A CN105296562A CN 105296562 A CN105296562 A CN 105296562A CN 201510733729 A CN201510733729 A CN 201510733729A CN 105296562 A CN105296562 A CN 105296562A
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- betaine
- phosphoric acid
- glutamic acid
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- hydrochloride
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Abstract
The invention discloses a glutamic acid fermentation culture medium using betaine phosphate to substitute betaine hydrochloride, and belongs to the technical field of biological fermentation. The glutamic acid fermentation culture medium comprises the following components by mass percent: 0.12 to 0.17 percent of potassium chloride, 0.05 to 0.1 percent of magnesium sulfate, 2 to 10 percent of initial sugar, 0.25 to 0.6 percent of corn steep liquor, 0.2 to 1 percent of soybean meal hydrolysate, 0.5 to 2 percent of phosphoric acid, 0.2 to 0.45 percent of betaine phosphate and the balance of water. According to the glutamic acid fermentation culture medium using the betaine phosphate to substitute the betaine hydrochloride, the betaine phosphate is used for substituting the betaine hydrochloride in the original culture medium, the chelating reaction of the phosphoric acid and the betaine hydrochloride is avoided, and the problem that the chelate precipitate damages a fermentation tank and is difficult to clear can be avoided; phosphorus elements necessary for the fermentation are contained in the betaine phosphate, so that the consumption of the phosphoric acid is reduced, and the cost is reduced.
Description
Technical field
The invention belongs to technical field of biological fermentation, be specifically related to a kind of glutamic acid fermentation substratum replacing betaine hydrochloride with phosphoric acid betaine.
Background technology
Glutamic acid fermentation is industrial at present to be applied often, and industrial employing glutamic acid fermentation produces mushroom, mainly contains corynebacterium glutamicum, brevibacterium lactofermentum, loose branch tyrothricin, brevibacterium flavum, bites ammonia tyrothricin etc.The current kind of total fermentation capacity of state's L-glutamic acid, close to 1,600,000 tons, accounts for 75% of global L-glutamic acid production capacity; And the L-glutamic acid production capacity of Japan, namely comprise Japanese Ajincomoto Co., Inc in native country and overseas branch office's production capacity in interior total only less than 600,000 tons; The glutamic acid fermentation ability of Korea S is at 200,000 ~ 250,000 tons.Add the glutamic acid fermentation ability of China Taiwan, can think, Asia L-glutamic acid manufacturer has dominated international L-glutamic acid market substantially.
Produce the fermentation of the L-glutamic acid of monosodium glutamate L-glutamic acid and so on, being different from traditional wine brewing and antibiotic fermentation, is a kind of metabolic control fermentation changing microbial metabolism.Glutamic acid fermentation substratum comprises carbon source, nitrogenous source, inorganic salt, somatomedin and water etc.Fermention medium be not only supply thalli growth breeding required for nutrition and energy, and be form L-glutamic acid carbon skeleton source.
Glutamic acid fermentation process needs certain substratum composition, and in the substratum that prior art uses, betaine hydrochloride is important integral part, and phosphoric acid is the important supplier of phosphoric in inorganic salt.Aborning, phosphoric acid easily and trimethyl-glycine chelating form magnesium ammonium phosphate and precipitate, certain difficulty is caused to the cleaning of equipment, the damage of quickening equipment, in addition because form the consumption that inner complex consumes trimethyl-glycine and phosphoric acid, add the usage quantity of trimethyl-glycine and phosphoric acid, add production cost.
Summary of the invention
The object of this invention is to provide a kind of glutamic acid fermentation substratum replacing betaine hydrochloride with phosphoric acid betaine, to solve the problem.
To achieve these goals, the technical solution used in the present invention is:
The present invention replaces the glutamic acid fermentation substratum of betaine hydrochloride with phosphoric acid betaine, comprises the component of following mass percent:
Repone K 0.12-0.17%, magnesium sulfate 0.05-0.1%, first sugared 2-10%, corn steep liquor 0.25-0.6%, soybean meal hydrolysate 0.2-1%, phosphoric acid 0.5-2%, phosphoric acid betaine 0.2-0.45%, all the other are water.
The described glutamic acid fermentation substratum replacing betaine hydrochloride with phosphoric acid betaine, comprises the component of following mass percent:
Repone K 0.14-0.15%, magnesium sulfate 0.075-0.085%, first sugared 4-8%, corn steep liquor 0.35-0.5%, soybean meal hydrolysate 0.4-0.75%, phosphoric acid 0.8-1.2%, phosphoric acid betaine 0.25-0.35%, all the other are water.
The present invention replaces the glutamic acid fermentation substratum of betaine hydrochloride with phosphoric acid betaine, also comprises Methionin 0.05-0.1%.
The described glutamic acid fermentation substratum replacing betaine hydrochloride with phosphoric acid betaine, comprises the component of following mass percent:
Repone K 0.14%, magnesium sulfate 0.08%, just sugar 6%, corn steep liquor 0.4%, soybean meal hydrolysate 0.6%, phosphatase 11 %, phosphoric acid betaine 0.3%, Methionin 0.08%, water 91.4%.
Beneficial effect of the present invention is: the present invention replaces the glutamic acid fermentation substratum of betaine hydrochloride with phosphoric acid betaine, employing phosphoric acid betaine substitutes the betaine hydrochloride in original substratum, avoid the chelatropic reaction of phosphoric acid and betaine hydrochloride, avoid inner complex precipitation to the damage of fermentor tank and the problem being difficult to cleaning; Implication in phosphoric acid betaine is fermented necessary phosphoric, and then decreases the usage quantity of phosphoric acid, reduces cost.
Embodiment
Embodiment 1
Replace the glutamic acid fermentation substratum of betaine hydrochloride with phosphoric acid betaine, comprise the component of following mass percent:
Repone K 0.17%, magnesium sulfate 0.1%, just sugar 10%, corn steep liquor 0.6%, soybean meal hydrolysate 1%, phosphoric acid 2%, phosphoric acid betaine 0.45%, water 85.68%.
Embodiment 2
Replace the glutamic acid fermentation substratum of betaine hydrochloride with phosphoric acid betaine, comprise the component of following mass percent:
Repone K 0.12%, magnesium sulfate 0.05%, just sugar 2%, corn steep liquor 0.25%, soybean meal hydrolysate 0.2%, phosphoric acid 0.5%, phosphoric acid betaine 0.2%, water 96.68%.
Embodiment 3
Replace the glutamic acid fermentation substratum of betaine hydrochloride with phosphoric acid betaine, comprise the component of following mass percent:
Repone K 0.14%, magnesium sulfate 0.075%, just sugar 4%, corn steep liquor 0.35%, soybean meal hydrolysate 0.4%, phosphoric acid 0.8%, phosphoric acid betaine 0.25%, water 93.985%.
Embodiment 4
Replace the glutamic acid fermentation substratum of betaine hydrochloride with phosphoric acid betaine, comprise the component of following mass percent:
Repone K 0.15%, magnesium sulfate 0.085%, just sugar 8%, corn steep liquor 0.5%, soybean meal hydrolysate 0.75%, phosphatase 11 .2%, phosphoric acid betaine 0.35%, water 88.965%.
Embodiment 5
Replace the glutamic acid fermentation substratum of betaine hydrochloride with phosphoric acid betaine, comprise the component of following mass percent:
Repone K 0.14%, magnesium sulfate 0.08%, just sugar 6%, corn steep liquor 0.4%, soybean meal hydrolysate 0.6%, phosphatase 11 %, phosphoric acid betaine 0.3%, Methionin 0.08%, water 91.4%.
Claims (4)
1. replace a glutamic acid fermentation substratum for betaine hydrochloride with phosphoric acid betaine, it is characterized in that, comprise the component of following mass percent:
Repone K 0.12-0.17%, magnesium sulfate 0.05-0.1%, first sugared 2-10%, corn steep liquor 0.25-0.6%, soybean meal hydrolysate 0.2-1%, phosphoric acid 0.5-2%, phosphoric acid betaine 0.2-0.45%, all the other are water.
2. the glutamic acid fermentation substratum replacing betaine hydrochloride with phosphoric acid betaine according to claim 1, is characterized in that, comprise the component of following mass percent:
Repone K 0.14-0.15%, magnesium sulfate 0.075-0.085%, first sugared 4-8%, corn steep liquor 0.35-0.5%, soybean meal hydrolysate 0.4-0.75%, phosphoric acid 0.8-1.2%, phosphoric acid betaine 0.25-0.35%, all the other are water.
3. the glutamic acid fermentation substratum replacing betaine hydrochloride with phosphoric acid betaine according to claim 1 and 2, is characterized in that, also comprise Methionin 0.05-0.1%.
4. the glutamic acid fermentation substratum replacing betaine hydrochloride with phosphoric acid betaine according to claim 3, is characterized in that, comprise the component of following mass percent:
Repone K 0.14%, magnesium sulfate 0.08%, just sugar 6%, corn steep liquor 0.4%, soybean meal hydrolysate 0.6%, phosphatase 11 %, phosphoric acid betaine 0.3%, Methionin 0.08%, water 91.4%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510733729.4A CN105296562A (en) | 2015-10-31 | 2015-10-31 | Glutamic acid fermentation culture medium using betaine phosphate to substitute betaine hydrochloride |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510733729.4A CN105296562A (en) | 2015-10-31 | 2015-10-31 | Glutamic acid fermentation culture medium using betaine phosphate to substitute betaine hydrochloride |
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| CN105296562A true CN105296562A (en) | 2016-02-03 |
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| CN201510733729.4A Pending CN105296562A (en) | 2015-10-31 | 2015-10-31 | Glutamic acid fermentation culture medium using betaine phosphate to substitute betaine hydrochloride |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101235401A (en) * | 2007-02-02 | 2008-08-06 | 上海祥韦思化学品有限公司 | Fermentation method for preparing L-amino acid |
| CN101457243A (en) * | 2009-01-06 | 2009-06-17 | 天津科技大学 | Novel process for improving L-glutamic acid fermentation production rate |
| CN103045668A (en) * | 2012-12-21 | 2013-04-17 | 菱花集团有限公司 | Glutamic acid fermentation medium replacing lysine with lysine fermentation liquid |
| CN103243131A (en) * | 2013-05-28 | 2013-08-14 | 山东祥维斯生物科技有限公司 | Method for preparing L-glutamic acid by fermentation |
| CN103243132A (en) * | 2013-05-28 | 2013-08-14 | 山东祥维斯生物科技有限公司 | Method for producing glutamic acid through double-feeding fermentation optimization of corn steep liquor and glucose |
-
2015
- 2015-10-31 CN CN201510733729.4A patent/CN105296562A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101235401A (en) * | 2007-02-02 | 2008-08-06 | 上海祥韦思化学品有限公司 | Fermentation method for preparing L-amino acid |
| CN101457243A (en) * | 2009-01-06 | 2009-06-17 | 天津科技大学 | Novel process for improving L-glutamic acid fermentation production rate |
| CN103045668A (en) * | 2012-12-21 | 2013-04-17 | 菱花集团有限公司 | Glutamic acid fermentation medium replacing lysine with lysine fermentation liquid |
| CN103243131A (en) * | 2013-05-28 | 2013-08-14 | 山东祥维斯生物科技有限公司 | Method for preparing L-glutamic acid by fermentation |
| CN103243132A (en) * | 2013-05-28 | 2013-08-14 | 山东祥维斯生物科技有限公司 | Method for producing glutamic acid through double-feeding fermentation optimization of corn steep liquor and glucose |
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Application publication date: 20160203 |