CN105288720A - Binding profile for vascular anastomosis - Google Patents
Binding profile for vascular anastomosis Download PDFInfo
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- CN105288720A CN105288720A CN201510675300.4A CN201510675300A CN105288720A CN 105288720 A CN105288720 A CN 105288720A CN 201510675300 A CN201510675300 A CN 201510675300A CN 105288720 A CN105288720 A CN 105288720A
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- vascular anastomosis
- section bar
- tack coat
- bonding section
- volume fraction
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Abstract
The scheme relates to a binding profile for vascular anastomosis. The binding profile comprises an absorbent layer and a binding layer, wherein the absorbent layer consists of a polylactic acid-glycolic acid copolymer, albumin and sterile water; the binding layer consists of albumin, chitosan, hydroxypropyl methyl cellulose, gelatin, sterile water and an optical absorbing dye; and the optical absorbing dye is selected from any combinations of two of the following materials: indocyanine green, methylene blue, carbon black, a cyanine dye CY7.5 and Alexa Fluor 790. According to the scheme, the two optical absorbing dyes, which are different in absorption wavelength, are organically combined, so that the combination of the optical absorbing dyes, in matching with double-wavelength laser, can be applied to the vascular anastomosis; and through a synergistic effect between the two dyes, energy from the laser can be well utilized for the vascular anastomosis on a position which is the closest to an anastomotic stoma on the basis of the principle that two different lasers are different in penetration depth.
Description
Technical field
The present invention relates to a kind of jointing material for vascular anastomosis, particularly a kind of bonding section bar for laser vascular anastomosis operation coordinating dual-wavelength laser to use.
Background technology
Vascular anastomosis refers to and fracture ends of vessels is connected, and makes it again to recover unobstructed operation method.In recent years, along with the increase gradually of vasodilation disorders and the sickness rate such as vascular occlusive disease and blood vessel injury, the technology of blood vessel transplantation, vascular suture and repairing is had higher requirement.Thin vessels and microvascular anastomosis, can the histoorgan being directly connected to blood vessel far-end survive, and occupies critical role in the field such as vascular surgery and microsurgery.Along with the development of microsurgery, the requirement of vascular anastomosis technology is being improved constantly, not only will have good instant patency rate, also will have better long-term patency rate and anti-tractive tension force etc.
The common method that clinical vascular coincide is sewing, and this method is applicable to large and mediumsize vessel, but when sewing up diameter and being less than the tiny blood vessels of lmm, not only difficulty is large, and amount is many, time-consuming, greatly have impact on the success rate of operation and applies widely.People to operation process can be simplified, shorten the mis instruments equipment of operating time and technical method with keen interest, and then there is series of new vascular anastomosis method.Such as, blood vessel anastomosis clamp/skill of handling needles, tissue glue's method, tiretube process and laser vascular anastomosis method etc.
At the end of the seventies, occurred laser vascular anastomosis art, attracting attention immediately appears in this technology one.When adopting laser to carry out vascular anastomosis, the heat effect of laser can make albuminous degeneration solidification in broken ends of fractured bone vascular cell, and then is attached on together.The penetration depth of laser is more shallow, can reduce the damage to tunica intima, is beneficial to the Reparation and Reconstruction of blood vessel.Although it is different that people carry out the technical equipment of experimentation, experimental design, object of study, observation detection means and result, obtain consistent approval.Namely laser vascular anastomosis art is that a kind of minimally-invasive vascular coincide method, and its potential clinical value is huge.
Utilize laser to carry out vascular anastomosis to have the following advantages compared with traditional suture: first, operation is simple, and the tissue ischemia time is short.The time of welding needed for every bar blood vessel is about the 1/3-1/4 of hands stitch, is easy to operation; Secondly, little to blood vessel injury, be beneficial to the post-operative recovery of blood vessel.Decrease suture to use, make to reduce the damage of blood vessel, blood vessel foreign body reaction degree alleviates greatly simultaneously, is beneficial to the recovery of post-surgical vascular; Finally, vascular patency is high, effectively can reduce granulation tissue hyperplasia, greatly reduces the probability that anastomotic stoma local anomaly blood flow is formed, and is conducive to keeping blood vessel unobstructed for a long time.
When applying laser and carrying out vascular anastomosis, except the laser used, a certain amount of identical agent is also needed to coordinate.These identical agent major parts are biological adhesive, such as fibrin, Fibrinogen etc., but the effect of these binding agents is not ideal enough, in order to the effect making laser vascular anastomosis reach best, also require accurate alignment anastomotic stoma edge.And blood vessel is flexible structure, cannot realize accurate docking, this can directly affect the quality of coincideing.Therefore, adopt necessary Ink vessel transfusing supporting construction, the quality promoting vascular anastomosis is necessary.But when only utilizing the laser of single wavelength to carry out vascular anastomosis, when optical maser wavelength long (more than 1 μm), the heat effect of laser is fairly obvious, and penetration depth is too shallow, be unfavorable for coincideing of blood vessel deep tissues; And if use short wavelength laser (such as 532nm) is although its penetration depth is deep, not obvious for superficial vein action effect, therefore, both are carried out organically combining just being provided with meaning.
Summary of the invention
For the deficiencies in the prior art part, the object of the invention is to for a bonding section bar supporting with it of dual-wavelength laser angiostomy exploitation.
For achieving the above object, the present invention is achieved through the following technical solutions:
For a bonding section bar for vascular anastomosis, comprise absorbed layer and tack coat;
Wherein, described absorbed layer includes Poly(D,L-lactide-co-glycolide, albumin and sterilized water;
Described tack coat comprises albumin, chitosan, hydroxypropyl emthylcellulose, gelatin, sterilized water and optical absorption dyestuff, and this optical absorption dyestuff is selected from any two kinds of combinations in indole cyanogen chlorine, methylene blue, white carbon black, flower cyanines uniformly dyeing material CY7.5 and AlexaFluor790.
Preferably, the described bonding section bar for vascular anastomosis, wherein, in described absorbed layer, the quality volume fraction of Poly(D,L-lactide-co-glycolide is 10 ~ 15%.
Preferably, the described bonding section bar for vascular anastomosis, wherein, in described absorbed layer, albuminous quality volume fraction is 30 ~ 45%.
Preferably, the described bonding section bar for vascular anastomosis, wherein, in described tack coat, albuminous quality volume fraction is 48 ~ 54%.
Preferably, the described bonding section bar for vascular anastomosis, wherein, in described tack coat, the quality volume fraction of chitosan is 3 ~ 8%.
Preferably, the described bonding section bar for vascular anastomosis, wherein, in described tack coat, the quality volume fraction of hydroxypropyl emthylcellulose is 1 ~ 2.5%.
Preferably, the described bonding section bar for vascular anastomosis, wherein, in described tack coat, the quality volume fraction of gelatin is 1 ~ 1.5%.
Preferably, the described bonding section bar for vascular anastomosis, wherein, in described tack coat, the quality volume fraction of optical absorption dyestuff is 0.25 ~ 3%.
The invention has the beneficial effects as follows: the optical absorption dyestuff of two kinds of different absorbing wavelength is carried out organic assembling by this case, make it that dual-wavelength laser can be coordinated to carry out vascular anastomosis, two kinds of different principles of the different laser penetration degree of depth are used, pressing close to most anastomotic stoma position, the energy that two kinds of dyestuff synergism more can utilize laser to provide preferably carries out vascular anastomosis.
Accompanying drawing explanation
Fig. 1 is the structural representation of the bonding section bar for vascular anastomosis.
Detailed description of the invention
Below in conjunction with accompanying drawing, the present invention is described in further detail, can implement according to this with reference to description word to make those skilled in the art.
Refer to Fig. 1, this case provides the bonding section bar for vascular anastomosis of an embodiment, comprises absorbed layer 1 and tack coat 2; The concrete wavelength chooses of this case to dual-wavelength laser does not limit, and can select according to actual needs, the present embodiment with wavelength be the laser of 808nm and 1064nm as a reference example.
Wherein, absorbed layer 1 includes Poly(D,L-lactide-co-glycolide, albumin and sterilized water;
Tack coat 2 comprises albumin, chitosan, hydroxypropyl emthylcellulose, gelatin, sterilized water and optical absorption dyestuff, and this optical absorption dyestuff is selected from any two kinds of combinations in indole cyanogen chlorine, methylene blue, white carbon black, flower cyanines uniformly dyeing material CY7.5 and AlexaFluor790.Such as, optical absorption dyestuff can be the combination of indole cyanogen chlorine and white carbon black, also can be the combination of white carbon black and Hua Jing uniformly dyeing material CY7.5, but the accounting of two kinds of dye combinations can not be limited, because both ratios can be carried out freely regulated according to the actual needs of angiostomy, the adjustment done is just a kind of adaptive adjustment also.
Although all contain water in absorbed layer 1 and tack coat 2, seeming is a kind of solution, in fact due to the existence of polymer, makes its entirety be rendered as a kind of semisolid paste state, and based on this, it just can be made as a kind of bonding section bar.Optical absorption dyestuff in tack coat 2 mainly take part in absorbing laser energy, and is translated into heat energy, for the anastomosis procedures of blood vessel provides the most direct heat energy.Produce heat energy so that albumin, chitosan, hydroxypropyl emthylcellulose, gelatin can be made to form a kind of network structure of complexity and produce certain stickiness, for the identical of blood vessel provides adhesion, the phenomenon preventing the blood vessel generation seepage after coincideing or break.Therefore, albumin, chitosan, hydroxypropyl emthylcellulose and gelatin work in coordination with existence each other, indivisible organic whole, plays an important role to the identical intensity of angiostomy.Absorbed layer 1 main component is Poly(D,L-lactide-co-glycolide and albuminous mixture, and these two kinds of complex have rigidity more, and its function supports protective layer as one, can prevent fracture and the distortion of tack coat.
In order to improve the effect of above-mentioned formula to vascular anastomosis further, need to limit wherein each constituent content.
In the above-described embodiments, in absorbed layer 1, the quality volume fraction (m/v × 100%) of Poly(D,L-lactide-co-glycolide is preferably 10 ~ 15%.If exceed this scope, the semi-solid state of absorbed layer will be affected, affect its docile effect to blood vessel.
In the above-described embodiments, in absorbed layer 1, albuminous quality volume fraction is preferably 30 ~ 45%.If exceed this scope, the rigidity of absorbed layer 1 will be affected, make it lose protective effect.
In the above-described embodiments, in tack coat 2, albuminous quality volume fraction is preferably 48 ~ 54%.This albumin can be preferably bovine serum albumin.If albuminous concentration exceeds preferred scope, reducing causing with the synergistic effect of chitosan, hydroxypropyl emthylcellulose and gelatin, stickiness being reduced, also cannot form effectively firm network structure.
In the above-described embodiments, in tack coat 2, the quality volume fraction of chitosan is preferably 3 ~ 8%.If the concentration of chitosan exceeds preferred scope, reducing causing with the synergistic effect of albumin, hydroxypropyl emthylcellulose and gelatin, stickiness being reduced, also cannot form effectively firm network structure.
In the above-described embodiments, in tack coat 2, the quality volume fraction of hydroxypropyl emthylcellulose is preferably 1 ~ 2.5%.If the concentration of hydroxypropyl emthylcellulose exceeds preferred scope, reducing causing with the synergistic effect of albumin, chitosan and gelatin, stickiness being reduced, also cannot form effectively firm network structure.
In the above-described embodiments, in tack coat 2, the quality volume fraction of gelatin is preferably 1 ~ 1.5%.If the concentration of gelatin exceeds preferred scope, reducing causing with the synergistic effect of albumin, chitosan and hydroxypropyl emthylcellulose, stickiness being reduced, also cannot form effectively firm network structure.
In the above-described embodiments, in tack coat 2, the quality volume fraction of optical absorption dyestuff is preferably 0.25 ~ 3%.If its content is on the low side, then cannot produce enough heats in the formula system at its place; If content is higher, heat will be caused excessive, blood vessel surface tissue will be had a negative impact.
Although embodiment of the present invention are open as above, but it is not restricted to listed in description and embodiment utilization, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other amendment, therefore do not deviating under the general concept that claim and equivalency range limit, the present invention is not limited to specific details and illustrates here and the legend described.
Claims (8)
1., for a bonding section bar for vascular anastomosis, comprise absorbed layer and tack coat;
Wherein, described absorbed layer includes Poly(D,L-lactide-co-glycolide, albumin and sterilized water;
Described tack coat comprises albumin, chitosan, hydroxypropyl emthylcellulose, gelatin, sterilized water and optical absorption dyestuff, and this optical absorption dyestuff is selected from any two kinds of combinations in indole cyanogen chlorine, methylene blue, white carbon black, flower cyanines uniformly dyeing material CY7.5 and AlexaFluor790.
2. the bonding section bar for vascular anastomosis according to claim 1, is characterized in that, in described absorbed layer, the quality volume fraction of Poly(D,L-lactide-co-glycolide is 10 ~ 15%.
3. the bonding section bar for vascular anastomosis according to claim 2, is characterized in that, in described absorbed layer, albuminous quality volume fraction is 30 ~ 45%.
4. the bonding section bar for vascular anastomosis according to claim 1, is characterized in that, in described tack coat, albuminous quality volume fraction is 48 ~ 54%.
5. the bonding section bar for vascular anastomosis according to claim 4, is characterized in that, in described tack coat, the quality volume fraction of chitosan is 3 ~ 8%.
6. the bonding section bar for vascular anastomosis according to claim 5, is characterized in that, in described tack coat, the quality volume fraction of hydroxypropyl emthylcellulose is 1 ~ 2.5%.
7. the bonding section bar for vascular anastomosis according to claim 6, is characterized in that, in described tack coat, the quality volume fraction of gelatin is 1 ~ 1.5%.
8. the bonding section bar for vascular anastomosis according to claim 7, is characterized in that, in described tack coat, the quality volume fraction of optical absorption dyestuff is 0.25 ~ 3%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510675300.4A CN105288720B (en) | 2015-10-19 | 2015-10-19 | Bonding proximate matter for vascular anastomosis |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510675300.4A CN105288720B (en) | 2015-10-19 | 2015-10-19 | Bonding proximate matter for vascular anastomosis |
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| CN105288720A true CN105288720A (en) | 2016-02-03 |
| CN105288720B CN105288720B (en) | 2018-07-27 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1935269A (en) * | 1998-06-18 | 2007-03-28 | 澳大利亚微研究基金会 | Compound for tissue repair and application thereof |
| US20090001122A1 (en) * | 2007-06-27 | 2009-01-01 | Megan Prommersberger | Buttress and surgical stapling apparatus |
| CN102238971A (en) * | 2008-09-19 | 2011-11-09 | 宾夕法尼亚大学董事会 | Solder formulation and use in tissue welding |
| CN102989035A (en) * | 2011-09-10 | 2013-03-27 | 浙江海正药业股份有限公司 | Hydrogel applicable to angiostomy and preparation method thereof |
-
2015
- 2015-10-19 CN CN201510675300.4A patent/CN105288720B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1935269A (en) * | 1998-06-18 | 2007-03-28 | 澳大利亚微研究基金会 | Compound for tissue repair and application thereof |
| US20090001122A1 (en) * | 2007-06-27 | 2009-01-01 | Megan Prommersberger | Buttress and surgical stapling apparatus |
| CN102238971A (en) * | 2008-09-19 | 2011-11-09 | 宾夕法尼亚大学董事会 | Solder formulation and use in tissue welding |
| CN102989035A (en) * | 2011-09-10 | 2013-03-27 | 浙江海正药业股份有限公司 | Hydrogel applicable to angiostomy and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 尹焕才等: "激光血管吻合技术研究进展", 《现代生物医学进展》 * |
| 范恒华等: "血管直接粘接吻合方法的实验研究", 《中华显微外科杂志》 * |
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| CN105288720B (en) | 2018-07-27 |
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