CN105267440A - Preparation technology of high EGCG low caffeine tea extract - Google Patents
Preparation technology of high EGCG low caffeine tea extract Download PDFInfo
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- CN105267440A CN105267440A CN201510653982.9A CN201510653982A CN105267440A CN 105267440 A CN105267440 A CN 105267440A CN 201510653982 A CN201510653982 A CN 201510653982A CN 105267440 A CN105267440 A CN 105267440A
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Abstract
The invention relates to a preparation technology of a high EGCG low caffeine tea extract. The technology specifically includes the steps of: (1) material selection: putting green tea sheets into a distillation kettle; (2) water extraction: conducting water extraction at 80-85DEG C for 30-40min; (3) subjecting the product to solid-liquid separation; (4) cooling the separated product to 30-35DEG C at room temperature; (5) filtering: conducting filtering with a 1000-mesh strainer to obtain a filtration product; (6) washing the filtered product with a resin loaded upper column; (7) first ethanol elution: adding 2-3 times volume of water into the washed product to conduct dilution, then adding ethanol that is 4-5 times the weight of the product to perform elution 4-5 times; (8) second ethanol elution: adding ethanol into the product obtained by the first ethanol elution treatment to conduct elution 2-3 times; and (9) carrying out concentration and drying on the product of the second ethanol elution to obtain a final product. The invention adopts a high EGCG low caffeine tea extract preparation technology, the processing process has no residue and high efficiency, and the product has obvious efficacy.
Description
Technical field
The present invention relates to a kind of high EGCG low-caffeine Folium Camelliae sinensis extract preparation technology.
Background technology
Catechin in Folium Camelliae sinensis is as an important class natural active matter, and particularly epigallocatechin gallate (EGCG) EGCG has more obvious blood fat reducing, blood sugar lowering, antioxidation, anticancer function, and food and medicine industry requirement amount increases year by year in recent years.The tradition technology of obtaining through refining mainly contains: organic solvent extraction: the method utilizes the dissolubility that in Folium Camelliae sinensis, different compound is different in organic solvent to carry out extraction and isolation.The method operation requirements is strict, have dissolvent residual in complex process, product.Precipitation by metallic ion method: the method utilizes tea polyphenols to generate crystalline deposit with some complexing of metal ion under certain condition, thus separate from lixiviating solution.The method three-protection design difficulty is large, easily causes environmental pollution, has metal residual in product.Supercritical fluid extraction method: utilize carbon dioxide to present liquids and gases state under the pressure and temperature of critical point, extract tea polyphenols in a liquid state.There is apparatus expensive, the low problem of yield in the method.Resin adsorption method: utilize resin with tea polyphenols, adsorbing characteristic can occur and carry out tea polyphenols and other component separating.Safe, nontoxic, efficient, the sustainable productive prospecting of the method.But traditional handicraft fails the effective catechin of disposable separation and caffeine, and after normally utilizing the method to make crude product tea polyphenols, then carry out the purification of effective catechin, the process-cycle is long, and production cost is high.
Summary of the invention
The deficiency that the present invention is directed to above problem provides a kind of technical scheme, utilizes resin absorption principle, adopts special technique, the disposable goods making high EGCG low caffeine content, course of processing noresidue, efficient, the peculiar effect of product is obvious, can be widely used in food and medicine industry.
Preparation process of the present invention is:
(1) select materials: green tea tablet is put into distillating still;
(2) flooding: add 80-85 DEG C of water, at this temperature flooding 30-40 minute in distillating still;
(3) product obtained above is carried out solid-liquid separation;
(4) at room temperature the product after separation is cooled to 30-35 DEG C;
(5) filter: filter with 1000 mesh filter screens, obtain the filtration product removing solid residue;
(6) by the upper prop washing that resin is housed of the product after filtration, water flow velocity is 5-7 m/h;
(7) first time ethanol elution: in the product after water washed, add 2-3 times of volume water dilution, then add ethanol elution 4-5 time of 4 ~ 5 times of products weight;
(8) second time ethanol elution: the product obtained after first time ethanol elution process adds certain density ethanol elution 2-3 time again;
(9) concentrate drying is carried out to the product after second time ethanol elution and obtain end product.
As improvement, the adsorbent resin in described step (6) can adopt H103, H1020, NKA-2, NKA-9.
As improvement, described first time the concentration of alcohol of ethanol elution be 20% ~ 30%.
As improvement, the concentration of alcohol of described second time ethanol elution is 60% ~ 70%.
As another improvement, effective catechin of described end product reaches more than 50%, and effective ratio more than 95%, content of caffeine is lower than 1%.
As another improvement, the concentrate drying method in described step (9) is spraying dry.
As further improving, the ethanol waste liquid after described eluting reaches concentration 70% recyclable recycling afterwards through concentrated.
Beneficial effect of the present invention: the invention provides a kind of high EGCG low-caffeine Folium Camelliae sinensis extract preparation technology, the effective catechin of product reaches more than 50%, effective ratio more than 95%, and content of caffeine is lower than 1%; The recyclable recycling of ethanol 70%, resin reproducible utilization, environmental protection; Can technical scaleization produce, fully the value of the Chinese low-grade tea of exploitation.
Detailed description of the invention
Embodiment 1
(1) select materials: green tea tablet is put into distillating still;
(2) flooding: add 80C water in distillating still, at this temperature flooding 30 minutes;
(3) product obtained above is carried out solid-liquid separation;
(4) at room temperature the product after separation is cooled to 30C;
(5) filter: filter with 1000 mesh filter screens, obtain the filtration product removing solid residue;
(6) by the upper prop washing that resin is housed of the product after filtration, water flow velocity is 5 ms/h; Adsorbent resin adopts H103;
(7) first time ethanol elution: in the product after water washed, add 2 times of volume water dilutions, then add the ethanol elution 4 times of 4 times of products weight; Concentration of alcohol is 20%;
(8) second time ethanol elution: the product obtained after first time ethanol elution process adds certain density ethanol elution 2 times again; Concentration of alcohol is 60%;
(9) carry out concentrate drying to the product after second time ethanol elution to obtain effective catechin and reach 60%, effective ratio 95%, the end product of content of caffeine 0.8%.
Embodiment 2
(1) select materials: green tea tablet is put into distillating still;
(2) flooding: add 83 DEG C of water in distillating still, at this temperature flooding 35 minutes;
(3) product obtained above is carried out solid-liquid separation;
(4) at room temperature the product after separation is cooled to 33C;
(5) filter: filter with 1000 mesh filter screens, obtain the filtration product removing solid residue;
(6) by the upper prop washing that resin is housed of the product after filtration, water flow velocity is 6 ms/h; Adsorbent resin adopts H103;
(7) first time ethanol elution: in the product after water washed, add 2.5 times of volume water dilutions, then add the ethanol elution 5 times of 5 times of products weight; Concentration of alcohol is 25%;
(8) second time ethanol elution: the product obtained after first time ethanol elution process adds certain density ethanol elution 3 times again; Concentration of alcohol is 65%;
(9) carry out concentrate drying to the product after second time ethanol elution to obtain effective catechin and reach 65%, effective ratio 96%, the end product of content of caffeine 0.7%.
Embodiment 3
(1) select materials: green tea tablet is put into distillating still;
(2) flooding: add 85 DEG C of water in distillating still, at this temperature flooding 40 minutes;
(3) product obtained above is carried out solid-liquid separation;
(4) at room temperature the product after separation is cooled to 35 DEG C;
(5) filter: filter with 1000 mesh filter screens, obtain the filtration product removing solid residue;
(6) by the upper prop washing that resin is housed of the product after filtration, water flow velocity is 7 ms/h; Adsorbent resin adopts H103;
(7) first time ethanol elution: in the product after water washed, add 3 times of volume water dilutions, then add the ethanol elution 5 times of 5 times of products weight; Concentration of alcohol is 30%;
(8) second time ethanol elution: the product obtained after first time ethanol elution process adds certain density ethanol elution 3 times again; Concentration of alcohol is 70%;
(9) carry out concentrate drying to the product after second time ethanol elution to obtain effective catechin and reach 70%, effective ratio 97%, the end product of content of caffeine 0.66%.
Claims (7)
1. a high EGCG low-caffeine Folium Camelliae sinensis extract preparation technology, is characterized in that, specifically comprise the steps:
(1) select materials: green tea tablet is put into distillating still;
(2) flooding: add 80-85 DEG C of water, at this temperature flooding 30-40 minute in distillating still;
(3) product obtained above is carried out solid-liquid separation;
(4) at room temperature the product after separation is cooled to 30-35 DEG C;
(5) filter: filter with 1000 mesh filter screens, obtain the filtration product removing solid residue;
(6) by the upper prop washing that resin is housed of the product after filtration, water flow velocity is 5-7 m/h;
(7) first time ethanol elution: in the product after water washed, add 2-3 times of volume water dilution, then add ethanol elution 4-5 time of 4 ~ 5 times of products weight;
(8) second time ethanol elution: the product obtained after first time ethanol elution process adds certain density ethanol elution 2-3 time again;
(9) concentrate drying is carried out to the product after second time ethanol elution and obtain end product.
2. a kind of high EGCG low-caffeine Folium Camelliae sinensis extract preparation technology as claimed in claim 1, it is characterized in that, the adsorbent resin in described step (6) can adopt H103, H1020, NKA-2, NKA-9.
3. a kind of high EGCG low-caffeine Folium Camelliae sinensis extract preparation technology as claimed in claim 1, is characterized in that: described first time the concentration of alcohol of ethanol elution be 20% ~ 30%.
4. a kind of high EGCG low-caffeine Folium Camelliae sinensis extract preparation technology as claimed in claim 1, is characterized in that: the concentration of alcohol of described second time ethanol elution is 60% ~ 70%.
5. a kind of high EGCG low-caffeine Folium Camelliae sinensis extract as claimed in claim 1 is prepared the effective catechin of work and is reached more than 50%, and effective ratio more than 95%, content of caffeine is lower than 1%.
6. a kind of high EGCG low-caffeine Folium Camelliae sinensis extract preparation technology as claimed in claim 1, is characterized in that: the concentrate drying method in described step (9) is spraying dry.
7. a kind of high EGCG low-caffeine Folium Camelliae sinensis extract preparation technology as claimed in claim 1, is characterized in that: the ethanol waste liquid after described eluting reaches concentration 70% recyclable recycling afterwards through concentrated.
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| CN201510653982.9A CN105267440A (en) | 2015-10-10 | 2015-10-10 | Preparation technology of high EGCG low caffeine tea extract |
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| CN201510653982.9A CN105267440A (en) | 2015-10-10 | 2015-10-10 | Preparation technology of high EGCG low caffeine tea extract |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109965049A (en) * | 2019-04-09 | 2019-07-05 | 汤臣倍健股份有限公司 | A kind of preparation method of the separation and Extraction effective component from green tea |
| CN111869769A (en) * | 2020-06-24 | 2020-11-03 | 浙江天草生物科技股份有限公司 | Production process of high-yield high-ester catechin green tea extract |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1939134A (en) * | 2005-09-30 | 2007-04-04 | 信阳市龙潭茶叶有限公司 | Low-cassaine cheese tea, low-cassaine cheese tea capsules thereof and preparation |
| CN101986855A (en) * | 2009-07-30 | 2011-03-23 | 温尧林 | Method for preparing low-caffeine high-tea polyphenol tea powder |
| CN103819444A (en) * | 2014-02-26 | 2014-05-28 | 成都华高生物制品有限公司 | Method for extracting monomeric EGCG from fresh tea |
| CN104171133A (en) * | 2014-08-06 | 2014-12-03 | 镇江市丹徒区茗缘茶叶专业合作社 | Method for preparing apple-flavored low-coffeine black tea |
-
2015
- 2015-10-10 CN CN201510653982.9A patent/CN105267440A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1939134A (en) * | 2005-09-30 | 2007-04-04 | 信阳市龙潭茶叶有限公司 | Low-cassaine cheese tea, low-cassaine cheese tea capsules thereof and preparation |
| CN101986855A (en) * | 2009-07-30 | 2011-03-23 | 温尧林 | Method for preparing low-caffeine high-tea polyphenol tea powder |
| CN103819444A (en) * | 2014-02-26 | 2014-05-28 | 成都华高生物制品有限公司 | Method for extracting monomeric EGCG from fresh tea |
| CN104171133A (en) * | 2014-08-06 | 2014-12-03 | 镇江市丹徒区茗缘茶叶专业合作社 | Method for preparing apple-flavored low-coffeine black tea |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109965049A (en) * | 2019-04-09 | 2019-07-05 | 汤臣倍健股份有限公司 | A kind of preparation method of the separation and Extraction effective component from green tea |
| CN111869769A (en) * | 2020-06-24 | 2020-11-03 | 浙江天草生物科技股份有限公司 | Production process of high-yield high-ester catechin green tea extract |
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Application publication date: 20160127 |