CN105267348A - Extraction method of plant composition containing radix polygoni multiflori and application - Google Patents
Extraction method of plant composition containing radix polygoni multiflori and application Download PDFInfo
- Publication number
- CN105267348A CN105267348A CN201510817094.6A CN201510817094A CN105267348A CN 105267348 A CN105267348 A CN 105267348A CN 201510817094 A CN201510817094 A CN 201510817094A CN 105267348 A CN105267348 A CN 105267348A
- Authority
- CN
- China
- Prior art keywords
- parts
- polygoni multiflori
- radix polygoni
- extraction
- vegetable composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides an extraction method of a plant composition containing radix polygoni multiflori. The plant composition is prepared from 100 parts of the radix polygoni multiflori, 50 parts of amphibious polygonum, 60 parts of herba sedi, 80 parts of galium verum and 70 parts of redflower peashrub roots through a microwave extraction method. The content is greatly increased, and dosage is reduced. The invention further provides application of the plant composition containing the radix polygoni multiflori in preparation of a drug for restraining human ovary adenocarcinoma SK-OV-3 cell proliferation.
Description
Technical field
The present invention relates to technical field of traditional Chinese medicine preparation, be specifically related to a kind of vegetable composition extracting method containing Radix Polygoni Multiflori and application.
Background technology
Containing the vegetable composition of Radix Polygoni Multiflori by Radix Polygoni Multiflori 100 parts, Herba Polygoni Amphibii 50 parts, careless Mus tooth half 60 parts, Herba galii veri 80 parts, Radix Caraganae Roseae 70 parts are made as crude drug, current extracting method decocts with water secondary, each 2-3 hour, collecting decoction, filter, be condensed into thick paste, granulate, dry, sugar coating, to obtain final product.
In prior art, not yet there is the vegetable composition containing Radix Polygoni Multiflori extracting the report adopting microwave technology in preparation, and adopt the method for soak by water, technique is coarse, backward, and impurity is many, causes patient's consumption excessive, be inconvenient to take, had a strong impact on this product and applied clinically.
Summary of the invention
Goal of the invention: in order to solve the problem, the object of the present invention is to provide a kind of vegetable composition extracting method containing Radix Polygoni Multiflori.
Another object of the present invention is to provide a kind of application of vegetable composition in preparation suppression people adenocarcinoma ovaries SK-OV-3 cell proliferation containing Radix Polygoni Multiflori.
Technical scheme: the object of the invention is by following scheme realize:
A kind of vegetable composition extracting method containing Radix Polygoni Multiflori, vegetable composition is by Radix Polygoni Multiflori 100 parts, Herba Polygoni Amphibii 50 parts, grass Mus tooth half 60 parts, Herba galii veri 80 parts, Radix Caraganae Roseae 70 parts is made as crude drug, preparation method is made up of the following step: get above-mentioned raw materials medicine, pulverize, add 70% ethanol of 2L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power is 400-600W, extract 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collect 5 times amount column volume eluents, decompression recycling ethanol, concentrated and dry, obtain microwave extraction thing, add customary adjuvant and be prepared into tablet, capsule or granule.
Above-mentioned a kind of vegetable composition extracting method containing Radix Polygoni Multiflori, in preparation method, microwave extracting power is 500W, each extraction 6 minutes.
A kind of vegetable composition Radix Polygoni Multiflori 100 parts containing Radix Polygoni Multiflori, Herba Polygoni Amphibii 50 parts, grass Mus tooth half 60 parts, Herba galii veri 80 parts, Radix Caraganae Roseae 70 parts is made as crude drug, preparation method is made up of the following step: get above-mentioned raw materials medicine, pulverize, add 70% ethanol of 2L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power is 400-600W, extract 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collect 5 times amount column volume eluents, decompression recycling ethanol, concentrated and dry, obtain microwave extraction thing, add customary adjuvant and be prepared into tablet, capsule or granule.
Above-mentioned a kind of vegetable composition containing Radix Polygoni Multiflori suppresses the application in people's adenocarcinoma ovaries SK-OV-3 cell proliferation in preparation, the power of microwave extracting described in preparation method is 500W, each extraction 6 minutes.
In prior art, the every sheet 0.4g of vegetable composition containing Radix Polygoni Multiflori, each 4,3 times on the one, adopt the every sheet 0.4g of vegetable composition containing Radix Polygoni Multiflori that the present invention is prepared into, but the medical material amount contained is original 2 times, therefore only needs 2 at every turn, within 1st, take 3 times, under the condition with more active component, greatly reduce dose.
Detailed description of the invention
Form by the following examples, foregoing of the present invention is described in further detail again, but this should be interpreted as that the scope of the above-mentioned theme of the present invention is only limitted to following example, all technology realized based on foregoing of the present invention all belong to scope of the present invention.
Embodiment 1
Get Radix Polygoni Multiflori 100 parts, Herba Polygoni Amphibii 50 parts, careless Mus tooth half 60 parts, Herba galii veri 80 parts, Radix Caraganae Roseae 70 parts, pulverize, add 70% ethanol of 2L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power is 400-600W, extract 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collects 5 times amount column volume eluents, decompression recycling ethanol, concentrated and dry, obtain microwave extraction thing, add customary adjuvant and be prepared into tablet, every sheet 0.4g.
Embodiment 2
Get Radix Polygoni Multiflori 100 parts, Herba Polygoni Amphibii 50 parts, careless Mus tooth half 60 parts, Herba galii veri 80 parts, Radix Caraganae Roseae 70 parts, pulverize, add 70% ethanol of 2L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power is 400-600W, extract 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collect 5 times amount column volume eluents, decompression recycling ethanol, concentrated and dry, obtain microwave extraction thing, add customary adjuvant and be prepared into capsule.
Embodiment 3
Get Radix Polygoni Multiflori 100 parts, Herba Polygoni Amphibii 50 parts, careless Mus tooth half 60 parts, Herba galii veri 80 parts, Radix Caraganae Roseae 70 parts, pulverize, add 70% ethanol of 2L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power is 400-600W, extract 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collect 5 times amount column volume eluents, decompression recycling ethanol, concentrated and dry, obtain microwave extraction thing, add customary adjuvant and be prepared into granule.
Herba Polygoni Amphibii is Polygonaceae arsesmart Herba Polygoni Amphibii PolygonumamphibiumL., with all herbal medicine.Xia Qiu gathers, and dries.Clearing away heat-damp and promoting diuresis.For dysentery; Furuncle is controlled in external.
Grass Mus tooth half is the herb of Crassulaceae sengreen Herba Stellariae Alsines SedumlineareThunb..The four seasons can adopt, and clean, using fresh herb or dry.This Fructus Adinae is Rosaceae avens geum aleppicum GeumaleppicumJacq., with all herbal medicine.
Herba galii veri is Rubiaceae clivers Herba galii veri GaliumverumL.var.asiaticumNakai, is used as medicine with herb and root.Xia Qiucai herb, autumn uproots.Clean chopping, using fresh herb or dry.Heat-clearing and toxic substances removing, promoting the circulation of blood, antipruritic, dampness removing; Treating the liver is scorching, throat moth is swollen and ache, treat that furuncle is swollen, paddy field dermatitis, urticaria, phlebitis, traumatic injury, women's vim and vigour pain etc.
Radix Caraganae Roseae is the root of leguminous plant Radix Caraganae Roseae.Spleen invigorating, kidney tonifying, stimulates the menstrual flow, diuresis, treats deficient consumptive fever, cough, stranguria with turbid discharge, sexual impotence, metrorrhagia, leucorrhea, and breast is few, uterine prolapse.Radix Polygoni Multiflori is pharmacopeia kind.
Embodiment 4: the vegetable composition containing Radix Polygoni Multiflori suppresses the experimentation data of people's adenocarcinoma ovaries SK-OV-3 cell proliferation
1 experiment material
1.1 experiment cell strains
People's adenocarcinoma ovaries SK-OV-3 cell, Nanjing Medical University's Experimental Animal Center laboratory cell storehouse, DMEM+10%FBS cellar culture.
1.2 Experimental agents
Drugs: the present invention is containing the vegetable composition of Radix Polygoni Multiflori: prepare by embodiment 1 method.
Medicinal liquid liquid storage: take 100mg containing the vegetable composition of Radix Polygoni Multiflori, be dissolved in 5ml dehydrated alcohol, 0.2 μm of frit, 500 μ ldoff pipe subpackages ,-20 DEG C of storage, 0.2 μm of frit dehydrated alcohol prepares against the use of matched group simultaneously.
1.3 experiment reagent
DMEM (GIBCO company Cat.No.12100-061Lot.No.758137); Hyclone (Tian Hang bio tech ltd, Zhejiang Lot.No.100419); NaHCO3 (Shanghai hundred million chemical reagent company limited Cat.No.11810-033Lot.No.1088387 of a specified duration); Trypsin (AMRESCO company lot number: 2010/04); EDTA (AMRESCO company lot number: 2009/10); PenicillinGSodiumSalt (AMRESCO company lot number: 2010242); StreptomycinSulfate (AMRESCO company lot number: 2010382); Dehydrated alcohol (Nanjing Chemistry Reagent Co., Ltd.'s lot number: 080310182); MTT (Biosharp lot number: 0793); PBS (laboratory autogamy);
1.4 experiment equipment
Lycra inverted microscope (German Leica model: DM1L); Visible-ultraviolet light microwell plate detector (MD company of U.S. model: SPECTRAMAX190); CO2 incubator (FORMA model: 3111); Super-clean bench (safe and sound Inc. of Su Jing group moulding number: SW-CJ-ZFD); Pure water instrument (Spring company of U.S. model: S/N020579); Accurate pipettor (French Gilson Inc model: P2); Electronic balance (German Sai Duolisi company limited model: BT323S); Full-automatic high-pressure autoclave (Japanese SANYO company model: MLS-3020); Table electrothermal air dry oven (the accurate experimental facilities company in Shanghai model: DHG9123A); Refrigerator (Siemens Company's model: KG18V21TI); Liquid nitrogen container (CBS model: 2001); Low speed centrifuge (Anting Scientific Instrument Factory, Shanghai's model: KA-1000); 0.2 μm of filter (MILLIPORE model: SLGP033RB); 10cm culture dish (NEST company), 96 well culture plates (NEST company); Cell counting count board; Centrifuge tube, pipet, Tips are some.
2 experimental techniques
1) people's adenocarcinoma ovaries SK-OV-3 cell DMEM+10%FBS in 37 DEG C, 5%CO2 carries out cellar culture (10cm culture dish), when Growth of Cells is to logarithmic (log) phase, collecting cell, discards culture fluid, PBS fine laundering 3 times, add 3ml0.25% trypsin-0.04%EDTA, after 37 DEG C of digestion 2min, add 5ml complete medium neutralization reaction wherein, proceeded in centrifuge tube after piping and druming cell, the centrifugal 5min of 1000rpm, adjustment concentration of cell suspension 3 × 10
4individual/ml.
2) enter in 96 well culture plates by cell kind, every hole adds cell suspension 180 μ l, and culture plate puts into cell culture incubator (37 DEG C, 5%CO2) cellar culture.
3) according to cell growth status, generally grow to 50%-70%, add the vegetable composition solution containing Radix Polygoni Multiflori, continue to cultivate 24h.
4) add 20 μ lMTT solution (5mg/ml, i.e. 0.5%MTT) after 24h, continue to cultivate 4h.
5) after 4h, buckle method removes supernatant, pats dry gently with absorbent paper, and every hole adds 200 μ l dimethyl sulfoxide, puts low-speed oscillation 10min on shaking table, crystal is fully dissolved.The light absorption value in each hole is measured at enzyme-linked immunosorbent assay instrument 490nm place.
6) background (do not add cell, only add culture fluid) is set, control wells (the medicine dissolution medium of cell, same concentrations, culture fluid, MTT, dimethyl sulfoxide) simultaneously, often organizes the multiple hole of setting 6.
7) result represents with the suppression ratio of medicine to cell:
Cell proliferation suppression ratio (%)=(control wells OD value-dosing holes OD value)/control wells OD value × 100%.Experiment repetition 3 times.
3 statistical dispositions
Adopt the correlation analysis in MicrosoftExcel2003 software and Studentt inspection, data represent with mean ± S.D..
4 experimental results
Statistical result showed after mtt assay experiment, compare with matched group, when dosage reaches 5mg/ml, to people's adenocarcinoma ovaries SK-OV-3 cell inhibitory effect variant (P<0.05), dosage this difference when 10mg/ml has significance (P<0.01), has pole significant difference (P<0.001) when dosage reaches 15-20mg/ml.
Table 1 contains the vegetable composition of Radix Polygoni Multiflori to people's adenocarcinoma ovaries SK-OV-3 cell inhibitory effect influence research (X ± SD)
Note: compare with matched group, * P<0.01; * P<0.001
5 experiment conclusion
Vegetable composition containing Radix Polygoni Multiflori can suppress people's adenocarcinoma ovaries SK-OV-3 cell proliferation, and reduce the Growth of Cells number of people's adenocarcinoma ovaries SK-OV-3 cell, this effect is dose dependent.
Claims (4)
1. the vegetable composition extracting method containing Radix Polygoni Multiflori, it is characterized in that vegetable composition is by Radix Polygoni Multiflori 100 parts, Herba Polygoni Amphibii 50 parts, grass Mus tooth half 60 parts, Herba galii veri 80 parts, Radix Caraganae Roseae 70 parts is made as crude drug, preparation method is made up of the following step: get above-mentioned raw materials medicine, pulverize, add 70% ethanol of 2L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power is 400-600W, extract 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collect 5 times amount column volume eluents, decompression recycling ethanol, concentrated and dry, obtain microwave extraction thing, add customary adjuvant and be prepared into tablet, capsule or granule.
2. a kind of vegetable composition extracting method containing Radix Polygoni Multiflori according to claim 1, is characterized in that in preparation method, microwave extracting power is 500W, each extraction 6 minutes.
3. one kind is being suppressed the application in people's adenocarcinoma ovaries SK-OV-3 cell proliferation containing the vegetable composition of Radix Polygoni Multiflori, it is characterized in that the vegetable composition containing Radix Polygoni Multiflori is by Radix Polygoni Multiflori 100 parts, Herba Polygoni Amphibii 50 parts, grass Mus tooth half 60 parts, Herba galii veri 80 parts, Radix Caraganae Roseae 70 parts is made as crude drug, preparation method is made up of the following step: get above-mentioned raw materials medicine, pulverize, add 70% ethanol of 2L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power is 400-600W, extract 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collect 5 times amount column volume eluents, decompression recycling ethanol, concentrated and dry, obtain microwave extraction thing, add customary adjuvant and be prepared into tablet, capsule or granule.
4. a kind of vegetable composition containing Radix Polygoni Multiflori, suppressing the application in people's adenocarcinoma ovaries SK-OV-3 cell proliferation, is characterized in that the power of microwave extracting described in preparation method is 500W, each extraction 6 minutes according to claim 3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510817094.6A CN105267348A (en) | 2015-11-23 | 2015-11-23 | Extraction method of plant composition containing radix polygoni multiflori and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510817094.6A CN105267348A (en) | 2015-11-23 | 2015-11-23 | Extraction method of plant composition containing radix polygoni multiflori and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105267348A true CN105267348A (en) | 2016-01-27 |
Family
ID=55137745
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510817094.6A Withdrawn CN105267348A (en) | 2015-11-23 | 2015-11-23 | Extraction method of plant composition containing radix polygoni multiflori and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105267348A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103860802A (en) * | 2014-03-14 | 2014-06-18 | 严白双 | Preparation method and application of Jieguqili tablet |
-
2015
- 2015-11-23 CN CN201510817094.6A patent/CN105267348A/en not_active Withdrawn
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103860802A (en) * | 2014-03-14 | 2014-06-18 | 严白双 | Preparation method and application of Jieguqili tablet |
Non-Patent Citations (2)
| Title |
|---|
| 程道海等: "广西产何首乌GXSW AQ-1对卵巢癌耐药细胞SKOV-3/CBP的耐药逆转作用研究", 《广西医科大学学报》 * |
| 黄志杰: "《中医抗癌古今验方精选》", 31 August 2009, 湖北科学技术出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103784738B (en) | A kind of vegetable composition extracting method containing Lepidinm meyenii Walp and application | |
| CN103816368B (en) | A kind of vegetable composition extracting method containing Radix Ginseng and application | |
| CN105030868A (en) | Extraction method for plant composition containing silybum marianum and application of plant composition | |
| CN103599292B (en) | A kind of preparation method of Guishaoliujun Pill and application | |
| CN103656600B (en) | A kind of preparation method of 12 WENJING WAN and application | |
| CN105055805A (en) | Extraction method and application of herbal composition containing herba andrographitis | |
| CN105125897A (en) | Extracting method and application of plant composite containing gastrodia elata | |
| CN105267348A (en) | Extraction method of plant composition containing radix polygoni multiflori and application | |
| CN105194002A (en) | Method for extracting plant composition containing echinacea and application of plant composition | |
| CN105250373A (en) | Extraction method and application of plant composition containing salvia miltiorrhiza | |
| CN103585615B (en) | A kind of preparation method of Dingkou Lizhong Wan and application | |
| CN105055587A (en) | Extraction method and application of plant composition containing ginkgo leaves | |
| CN105147869A (en) | Extracting method and application of plant composition containing green tea | |
| CN105106570A (en) | Extraction method and application of garlic-containing plant combination | |
| CN105267681A (en) | Extraction method and application of plant composition containing ginseng | |
| CN103656016B (en) | A kind of preparation method of antiasthmatic pill of perilla fruit and application | |
| CN105106264A (en) | Extraction method and application of plant composition containing shiitake | |
| CN103655923B (en) | A kind of preparation method of Auricularia SHUJIN WAN and application | |
| CN105343288A (en) | Extraction method and application of plant composition containing Cortex Cinnamomi | |
| CN103610857B (en) | A kind of preparation method and application of returning red pill for traumatic injuries | |
| CN105106331A (en) | Extraction method and application of plant composition containing oenothera biennis | |
| CN105147842A (en) | Extracting method and application of plant composition containing radix pseudostellariae | |
| CN105168433A (en) | Extracting method and application of plant composition containing gynostemma pentaphylla | |
| CN105147843A (en) | Method for extracting plant composition containing angelica and application of plant composition | |
| CN105125989A (en) | Extracting method and application of plant composite containing hawthorns |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20160127 |
|
| WW01 | Invention patent application withdrawn after publication |