CN105267299A - Perilla flavone and perilla flavone derivative extraction method - Google Patents
Perilla flavone and perilla flavone derivative extraction method Download PDFInfo
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Abstract
The present invention discloses a perilla flavone and perilla flavone derivative extraction method comprising the following steps: (1) selecting perilla leaves, grinding and breaking with a mortar, and sieving; (2) putting the ground and sieved perilla leaves into an ultrasonic extraction machine tank, and adding 30-80% by mass of ethanol, wherein the solid-liquid ratio is 1: 30-50, the water bath temperature is 40-80 DEG C, water bath time is 0.5-2.5 hours, and ultrasound time is 20-60min, (3) centrifuging the mixture obtained by the step 1to2 at 12000g, and removing the precipitate to obtain a perilla flavone and perilla flavone derivative solution. The yield of the perilla flavone and perilla flavone derivative extracted by the method is 78.2mg / g. The obtained perilla flavone has broad-spectrum antimicrobial activity, has good gram negative bacteria and gram positive bacteria antibacterial activity, has the 24h inhibition rate reaching 90% or more, and has broad prospects for development.
Description
Technical field
The present invention relates to technical field of chemistry, be specifically related to the extracting method of flavone and derivant thereof in Folium Perillae.
Background technology
Folium Perillae (Perillafrutescens), has another name called fragrant Soviet Union, is Labiatae herbaceous plant, has the cultivation history of more than 2000 year in China, is one of first batch of traditional drugs, food dual purpose plant [1-3] that health ministry is promulgated.Research display, multiple positions of Folium Perillae are all containing abundant nutrient substance and active substance.Wherein, 8 kinds of essential amino acids are contained in Folium Perillae stem, leaf.Fructus Perillae oil content is 30%-51% and mostly is unsaturated fatty acid, and wherein alpha-linolenic acid content is 64.73%, linoleic acid is 15.43%, oleic acid is 12.01%, is a kind of desirable edible oil raw material.Folium perillae extract has inflammation-inhibiting reaction and antipruritic effect.
At present, extract about Folium Perillae active substance and get more and more with the research of application, but at present perilla oil aspect is mainly concentrated on to the research of Folium Perillae, and relatively less about the report of perilla flavone.
In order to solve above-mentioned technical problem, the present invention comes therefrom.
Summary of the invention
First aspect technical problem to be solved by this invention is to provide the extracting method of flavone and derivant thereof in a kind of Folium Perillae.
In order to solve above-mentioned technical problem, the invention provides the extracting method of flavone and derivant thereof in a kind of Folium Perillae, it is characterized in that, it comprises the steps:
(1) Folium Perillae blade is chosen, broken by mortar grinder, sieve;
(2) Folium Perillae that grinding is sieved is put into the groove of ultrasonic extractor, adding mass percent is 30-80% ethanol, and solid-liquid ratio is 1:30-50, bath temperature 40 ~ 80 DEG C, water bath time 0.5-2.5 hour, ultrasonic time 20-60min,
(3) mixture 12000g abovementioned steps obtained is centrifugal, removes precipitation, obtains the solution of flavone and derivant thereof.
In the inventive solutions, in described step (2), adding mass percent is 30-50% ethanol.
In the inventive solutions, in described step (2), adding mass percent is 40% ethanol.
In the inventive solutions, in described step (2), after adding ethanol, solid-liquid ratio is 1:40.
In the inventive solutions, in described step (2), bath temperature 50 ~ 70 DEG C, water bath time 0.5-1.0 hour.
In the inventive solutions, in described step (2), ultrasonic time 30-50min.
In the inventive solutions, in described step (2), ultrasonic time 40min.
In the inventive solutions, in described step (3), the mixture 12000g obtained after step (2) being processed is centrifugal.
Chromocor derivative or flavone compound make a general reference a series of phenolic compounds that two phenyl ring with phenolic hydroxyl group are linked by center thricarbon atom, and its basic parent nucleus is 2-phenyl chromone.The functional groups such as phenolic hydroxyl group, methyl, methoxyl group, isopentene group are often connected with in chromocor derivative structure.In addition, it also normal and sugar be combined into glycosides.Research herein finds that perilla flavone and derivant thereof have significant scavenging action to hydroxy radical and oxygen-derived free radicals, and Scavenging activity is all proportionate with concentration.When the concentration of flavone in Folium Perillae is 2.00mg/ml, reaching 82.5% and 75.2% respectively to the clearance rate of hydroxy radical and oxygen-derived free radicals, is that the Scavenging activity of the ascorbic acid Vc solution of 0.75mg/ml is suitable with concentration.In Folium Perillae, flavone compound also has good scavenging action to DPPH, but not as ascorbic acid successful.Free radical is the compound produced in human metabolism's process, has strong oxidizing property, and body self has radical scavenging activity.But the free radical once body accumulates too much and eliminating activity intravenousY declines, and the tissue of body and cell will suffer damage, and then cause disease and aging.Result of study herein shows that perilla flavone compounds has the activity of scavenging free radicals, illustrates that Folium Perillae can be developed as the antidotal natural activity medicine of one.
In the present invention, total flavones refers to and comprises flavone and chromocor derivative.
The extracting method of flavone and derivant thereof in Folium Perillae of the present invention, when extraction process is concentration of alcohol 40%, solid-liquid ratio is 1:30, ultrasonic time 40min, bath temperature 70 DEG C, and the yield extracting flavone and derivant thereof in Folium Perillae under this condition is 78.2mg/g.In prior art, usual perilla flavone yield is 50-60mg/g.The present invention adopts ultrasonic extraction, and selects Folium Perillae to extract flavone wherein, improves flavone yield.
In recent years, antibiotics resistance sex chromosome mosaicism is more and more outstanding.Development of new antibiotic formulations is particularly important for solution drug resistance problems.Flavonoid and there is the Antibacterial Mechanism different from conventional antibiotic.Flavone compound can play antibacterial action by the virulence factor of direct bacteriostasis, anti-bacteria and with antibiotic synergism.Study discovery herein, perilla flavone has broad spectrum antibiotic activity, and can all have good bacteriostatic activity to gram negative bacteria and gram-positive bacterium, 24h bacteriostasis rate can reach more than 90%.Perilla flavone compounds is a kind of natural antibacterial medicine, has wide DEVELOPMENT PROSPECT.
Accompanying drawing explanation
Fig. 1 is rutin standard curve.
Fig. 2 is the influence curve figure of concentration of alcohol to total flavones extraction ratio.
Fig. 3 is the influence curve figure of solid-liquid ratio to total flavones extraction ratio.
Fig. 4 is the influence curve figure of ultrasonic time to total flavones extraction ratio.
Fig. 5 is the influence curve figure of bath temperature to total flavones extraction ratio.
Fig. 6 is the influence curve figure of water bath time to total flavones extraction ratio.
Fig. 7 is DPPH. clearance curve figure.
Fig. 8 is Hydroxyl radical-scavenging curve chart.
Fig. 9 is the clearance curve figure of oxygen-derived free radicals.
Figure 10 is that flavone is to escherichia coli fungistatic effect figure.
Figure 11 is that flavone is to staphylococcus aureus fungistatic effect figure.
Detailed description of the invention
Below in conjunction with drawings and Examples, the present invention will be described in more detail.
According to following example item, can better understand the present invention.But those skilled in the art is easier to understand, concrete material ratio, process conditions and result thereof described in enforcement only for illustration of the present invention, and should can not limit the present invention described in detail in claim yet.
Folium Perillae, rutin are purchased from Jian Zhong source, Jiangsu Chinese medicine company limited.Reagent used in test is domestic analytical pure.
Rutin standard curve
Get rutin standard substance 5.3mg, the rutin titer of configuration 0.212mg/ml.With concentration (C) for abscissa, with absorbance (A) for vertical coordinate, drawing standard curve (Fig. 1), obtains regression equation: y=0.8149x-0.0037, R2=0.9802.Result shows, rutin is good linear relationship at 0.01-0.1mg/ml.
One, the extracting method of perilla flavone and derivant thereof
Embodiment 1:
According to the technical scheme extracting method of summary of the invention, take ethanol as solvent, investigate ethanol contend number (30%, 40%, 50%, 60%, 70%, 80%), impact on chromocor compound yield respectively.The mensuration of flavone concentration adopts rutin standard curve to coordinate absorbance to carry out.Chromocor compound yield computing formula is:
Folium Perillae total flavones yield=(in crude extract flavone total amount/Folium Perillae quality) × 100%, following account form is same.
As shown in Figure 2, concentration of alcohol and the curved relation of flavone yield, and the ethanol extraction flavone yield of variable concentrations difference is larger.Concentration of alcohol flavone yield 30% ~ 40% time sharply increases; Yield relatively stable (difference is only 0.06%) when concentration is 40% ~ 60%; Concentration is 60% ~ 80% extraction ratio rapid drawdown.Therefore concentration 40% ~ 60% ethanol is selected to carry out orthogonal experiment.
Embodiment 2
According to the technical scheme extracting method of summary of the invention, take ethanol as solvent, investigate ethanol solid-liquid ratio (1:20,1:30,1:40,1:50) respectively to the impact of chromocor compound yield.Computing formula and computational methods the same.As shown in Figure 3, along with the increase of solid-liquid ratio, flavone yield progressively improves.When solid-liquid ratio is 1:40, total flavones has most high yield pulp1 to reach 3.32%; When solid-liquid ratio is 1:50, extraction ratio slightly reduces.So choosing 1:30,1:40,1:50 is the condition that orthogonal test is investigated.
Embodiment 3
According to the technical scheme extracting method of summary of the invention, select different ultrasonic treatment time (20,30,40,50,60min) investigate impact on chromocor compound yield.
As shown in Figure 4, during ul-trasonic irradiation time 20-40, flavone yield increases gradually.Because hyperacoustic cavitation and oscillation action are conducive to the stripping of intracellular matter.But when sonication treatment time starts to decline more than extraction ratio during 40min, this may be because long-time supersound process destroys structure and the biological activity of flavone.Therefore ultrasonic time 30 ~ 50min is selected to carry out orthogonal experiment.
Embodiment 4
According to the technical scheme extracting method of summary of the invention, select the different impact of bath temperature (40,50,60,70,80 DEG C) investigation on chromocor compound yield.
As shown in Figure 5, along with the rising of extraction temperature, extracting flavonoids rate progressively rises, extraction comprises the processes such as infiltration, dissolving and diffusion, and raising flavone dissolubility in ethanol with temperature increases, and lixiviating solution viscosity reduces, diffusion coefficient increases, and impels leaching rate to accelerate.When temperature reaches 70 DEG C, total flavones extraction rate reached is to maximum, and temperature raises total flavones extraction ratio again and declines to some extent on the contrary.When temperature is too high, thermal energy consumption is large, and easily cause solvent volatilization loss, the active component in extracting solution is also easily destroyed, and the stripping quantity of impurity increases, and makes troubles to subsequent operation, and cost increases.Therefore, from total flavones biological activity, minimizing solvent volatilizees and the angle of reduction energy consumption considers, and temperature during extraction preferably controls at 50 ~ 70 DEG C.
Embodiment 5
According to the technical scheme extracting method of summary of the invention, select different water bath time (0.5,1.0,1.5,2.0,2.5h) investigate impact on chromocor compound yield.
As shown in Figure 6, the growth of extraction time is conducive to flavone and diffuses out fully, total flavones extraction ratio increases gradually, after 1h, when diffusion reaches balance, time lengthening can make extracting section flavone out be adsorbed by blade once again, and in lixiviating solution, flavone concentration is basic reaches balance with flavone concentration in blade, the impact reduction of extracting effect.From the viewpoint of saving time, reducing energy consumption and the volatilization of minimizing solvent, extraction time should control at 1h.
Embodiment 6
In order to carry out deep research to ultrasound assisted extraction technique, on the basis of single factor experiment, select concentration of alcohol, solid-liquid ratio, ultrasonic time, bath temperature as investigation factor, each selecting factors 3 levels, establishment L9 (34) orthogonal table carries out testing (table 1), with flavone yield for inspection target.
Table 1 orthogonal test factor level designs
Can be obtained from table 2 range analysis, the primary and secondary order that each factor affects total flavones extraction ratio is D (bath temperature) >A (concentration of alcohol) >B (solid-liquid ratio) >C (ultrasonic time), and A1B3C2D3 extracting flavonoids rate is the highest.In view of only having bath temperature to there is horizontal appreciable impact to extraction effect, and concentration of alcohol, ultrasonic time and solid-liquid ratio have no significant effect extraction effect, and the impact of solid-liquid ratio on extraction effect is minimum.From reducing costs consideration, B3 is changed B1, determine that extraction process is A1B1C2D3 thus, namely optimum extraction process is concentration of alcohol 40%, and solid-liquid ratio is 1:30, ultrasonic time 40min, bath temperature 70 DEG C, and under this condition, the yield of Folium Perillae flavone is 3.59%.
Table 2 orthogonal and result
Two, antibacterial, the antioxidant activity analysis of perilla flavone
2.1 perilla flavones are to the Scavenging activity of DPPH
Ethanol with 60% prepare respectively mass concentration 0.25,0.50,1.00, the flavonoids solution of 2.00mg/ml.The each strength solution 2.0mL of accurate absorption is in test tube, add the DPPH solution 2.0ml of 2 × 10-4mol/L, shake up, leave standstill 30min, at 517nm place, the method measuring the absorbance A c of the alcohol mixeding liquid of the absorbance A i of sample, the absorbance A j of 2.0ml sample and 2.0ml60% alcohol mixeding liquid, 2.0mlDPPH. solution and 2.0ml60% respectively same measures ascorbic acid (V) to the clearance rate of DPPH.Often organize and 3 repetitions be all set, average and be calculated as follows the clearance rate of DPPH:
Clearance rate=[1-(Ai-Aj)/Ac] × 100%
In formula: Ai is the absorbance of sample; Aj is the absorbance of sample and alcohol mixeding liquid;
Ac is the absorbance of DPPH solution and alcohol mixeding liquid
As seen from Figure 7, perilla flavone has good scavenging action to DPPH, and when flavone concentration is less than 1mg/mL, increase with concentration, scavenging action enlarges markedly; But after flavone concentration is greater than 1mg/ml, each group removes the ability of DPPH all not as ascorbic acid Vc.
2.2 perilla flavones are to the Scavenging activity of hydroxy radical
Ethanol with 60% prepare respectively mass concentration be 0.25,0.50,1.00, the flavonoids solution of 2.00mg/ml.Getting concentration is that 0.75mmol/L orthophenanthroline solution 1ml is in test tube, add 2ml0.2mol/L phosphate buffer (pH7.40), 1ml distilled water, the FeSO4 solution of 1ml0.75mmol/L, 1ml mass fraction is the H2O2 of 0.12%, mixing, 37 DEG C of water-bath 90min, measuring absorbance in 536nm place is Ap, and replacing 1mlH2O2 to measure absorbance with 1ml distilled water is Ab; Replace the distilled water of 1ml to measure absorbance with sample solution to be As, often to organize parallel assay and average for 3 times, be calculated as follows the clearance rate of sample to hydroxyl, replace sample as a control group with variable concentrations Vc solution.
Clearance rate=(As-Ap)/(Ab-Ap) × 100%
In formula: Ap measures absorbance for adding H2O2; Ab is that distilled water replaces H2O2 to measure absorbance;
As is that sample solution replaces distilled water to measure absorbance.
Fenton reaction produces hydroxy radical: H2O2+Fe3+ → OH+OH-+Fe3+, the F2+ in system and orthophenanthroline generates red complex, has obtained the maximum absorption at 536nm place.Antioxidant inhibits Fe2+ to be oxidized, thus decreases the generation of OH, and changes the extent of reaction of Fe2+ orthophenanthroline, causes absorbance to change.As seen from Figure 8, perilla flavone compounds and ascorbic acid (Vc) have significant scavenging action to hydroxy radical, Scavenging activity strengthens with the increase of perilla flavone concentration, when in Folium Perillae, chromocor compound mass concentration is 1.00mg/mL, can reach 60% to the clearance rate of hydroxy radical, be that the Scavenging activity of 0.7mg/ml ascorbic acid solution is substantially suitable with concentration.
2.3 perilla flavones are to the Scavenging activity of oxygen-derived free radicals
Ethanol with 60% prepare respectively mass concentration be 0.25,0.50,1.00, the flavone compound solution of 2.00mg/ml.Get the Tris-HCI buffer 4.5ml of 0.05mol/LpH8.2, be placed in 25 DEG C of water-bath preheating 20min, add the pyrogallol solution of 1.0ml sample solution and 0.4ml25mmol/L respectively, mixing, 25 DEG C of water-bath 5min, add 8mo/lHCl1.0ml cessation reaction, make reference with Tris-HC1 buffer, measure absorbance in 325nm place, calculate clearance rate.Blank group replaces sample solution with 1.0ml alcoholic solution.Often organize parallel assay to average for 3 times, calculation sample, to the clearance rate of ultra-oxygen anion free radical, replaces sample as a control group with variable concentrations Vc solution.
Clearance rate (%)=(A1-A2)/A1 × 100%
In formula: A1 is blank mean light absorbency, and A2 is the mean light absorbency of sample
Pyrogallol A oxidation Decomposition in weak alkaline medium produces O2-, utilizes O2-scavenger that mouse thymus cells product can be made to be suppressed this feature at the absworption peak at 325nm place and measures.
As seen from Figure 9, in Folium Perillae, flavone and ascorbic acid (Vc) have good scavenging action to oxygen-derived free radicals, along with the increase of flavone concentration, scavenging action is strengthened, when in Folium Perillae, chromocor compound mass concentration is 2.00mg/mL, can reach flavone sample and concentration in the Folium Perillae of 75.2%, 1.00mg/ml to the clearance rate of oxygen-derived free radicals is that the Scavenging activity of the ascorbic acid Vc solution of 0.5mg/mL is suitable.
2.4 perilla flavone bacteriostatic activities are analyzed
With the perilla flavone solution of LB culture fluid configuration variable concentrations, concentration is respectively 1.0,0.5,0.25,0.125,0.0625mg/ml, ultraviolet sterilization.The perilla flavone solution getting variable concentrations joins in the escherichia coli and staphylococcus aureus bacterium liquid being in exponential phase, is 0.1 to OD600.Getting 100 μ L above-mentioned dilution bacterium liquid is spread evenly across on LB flat board, is inverted cultivation 24 hours at 37 DEG C; The bacterium liquid 100 μ L separately getting LB dilution coats on LB flat board as blank.
IR=[(matched group viable bacteria bacterium amount one test group viable bacteria bacterium amount)/matched group viable bacteria bacterium amount] × 100%
Antibacterial result display, perilla flavone all has good fungistatic effect for gram-positive bacterium and gram negative bacteria, and there is obvious concentration-dependent relation, along with the increase fungistatic effect of perilla flavone concentration more obvious (Figure 10,11), may be because flavone concentration is larger, larger with the contact surface of bacterium liquid, therefore fungistatic effect is better.The minimal inhibitory concentration of perilla flavone to escherichia coli and staphylococcus aureus is respectively 0.25mg/ml and 0.5mg/ml (table 3).
Table 3 perilla flavone is to escherichia coli and the dull and stereotyped antibacterial result of staphylococcus aureus
More than show and describe ultimate principle of the present invention, principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not by the restriction of above-mentioned example; what describe in above-mentioned example and description just illustrates principle of the present invention; the present invention also has various changes and modifications without departing from the spirit and scope of the present invention, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof.
Claims (8)
1. the extracting method of flavone and derivant thereof in Folium Perillae, it is characterized in that, it comprises the steps:
(1) Folium Perillae blade is chosen, broken by mortar grinder, sieve;
(2) Folium Perillae that grinding is sieved is put into the groove of ultrasonic extractor, adding mass percent is 30-80% ethanol, and solid-liquid ratio is 1:30-50, bath temperature 40 ~ 80 DEG C, water bath time 0.5-2.5 hour, ultrasonic time 20-60min,
(3) mixture 10000-15000g abovementioned steps obtained is centrifugal, removes precipitation, obtains the solution of flavone and derivant thereof.
2. the extracting method of flavone and derivant thereof in Folium Perillae according to claim 1, it is characterized in that, in described step (2), adding mass percent is 30-50% ethanol.
3. the extracting method of flavone and derivant thereof in Folium Perillae according to claim 1, it is characterized in that, in described step (2), adding mass percent is 40% ethanol.
4. the extracting method of flavone and derivant thereof in Folium Perillae according to claim 1, it is characterized in that, in described step (2), after adding ethanol, solid-liquid ratio is 1:40.
5. the extracting method of flavone and derivant thereof in Folium Perillae according to claim 1, is characterized in that, in described step (2), and bath temperature 50 ~ 70 DEG C, water bath time 0.5-1.0 hour.
6. the extracting method of flavone and derivant thereof in Folium Perillae according to claim 1, is characterized in that, in described step (2), and ultrasonic time 30-50min.
7. the extracting method of flavone and derivant thereof in Folium Perillae according to claim 1, is characterized in that, in described step (2), and ultrasonic time 40min.
8. the extracting method of flavone and derivant thereof in Folium Perillae according to claim 1, is characterized in that, in described step (3), the mixture 12000g obtained after step (2) being processed is centrifugal.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106975240A (en) * | 2017-05-09 | 2017-07-25 | 福建农林大学 | A kind of integrated approach that flavones is extracted from purple perilla |
| CN109744453A (en) * | 2019-03-20 | 2019-05-14 | 金华职业技术学院 | A kind of preparation method of extractive of perilla freeze-dried powder, compounding purple perilla antioxidant and its application |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101278970A (en) * | 2008-05-23 | 2008-10-08 | 昆明理工大学 | Method for preparing Elsholtzia bodinieri flavonoid and applications |
| CN101544991A (en) * | 2008-03-28 | 2009-09-30 | 湖北中烟工业有限责任公司 | Natural antioxidant of perilla flavone for cigarette and method for preparing same |
| CN103110834A (en) * | 2012-12-19 | 2013-05-22 | 盐城工学院 | Process for extracting partina anglica general flavone through supercritical CO2 method |
| CN104173438A (en) * | 2014-09-05 | 2014-12-03 | 南京泽朗生物科技有限公司 | Preparation method of general flavone of purple perilla |
| CN104523767A (en) * | 2014-12-16 | 2015-04-22 | 南京化工职业技术学院 | Method for extracting flavones substances in cacumen biotae |
| CN104887723A (en) * | 2015-06-18 | 2015-09-09 | 天津理工大学 | Method for extracting flavonoids from ginkgo biloba leaves with deep eutectic solvent |
-
2015
- 2015-09-22 CN CN201510608476.8A patent/CN105267299A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101544991A (en) * | 2008-03-28 | 2009-09-30 | 湖北中烟工业有限责任公司 | Natural antioxidant of perilla flavone for cigarette and method for preparing same |
| CN101278970A (en) * | 2008-05-23 | 2008-10-08 | 昆明理工大学 | Method for preparing Elsholtzia bodinieri flavonoid and applications |
| CN103110834A (en) * | 2012-12-19 | 2013-05-22 | 盐城工学院 | Process for extracting partina anglica general flavone through supercritical CO2 method |
| CN104173438A (en) * | 2014-09-05 | 2014-12-03 | 南京泽朗生物科技有限公司 | Preparation method of general flavone of purple perilla |
| CN104523767A (en) * | 2014-12-16 | 2015-04-22 | 南京化工职业技术学院 | Method for extracting flavones substances in cacumen biotae |
| CN104887723A (en) * | 2015-06-18 | 2015-09-09 | 天津理工大学 | Method for extracting flavonoids from ginkgo biloba leaves with deep eutectic solvent |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106975240A (en) * | 2017-05-09 | 2017-07-25 | 福建农林大学 | A kind of integrated approach that flavones is extracted from purple perilla |
| CN109744453A (en) * | 2019-03-20 | 2019-05-14 | 金华职业技术学院 | A kind of preparation method of extractive of perilla freeze-dried powder, compounding purple perilla antioxidant and its application |
| CN109744453B (en) * | 2019-03-20 | 2022-06-07 | 金华职业技术学院 | Preparation method of perilla extract freeze-dried powder, compound perilla antioxidant and application of compound perilla antioxidant |
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Application publication date: 20160127 |