CN105267135A - Method using BacillusamyloliquefaciensH47 for making mulberry leaf apocynum venetum leaf enzyme hair care cream - Google Patents
Method using BacillusamyloliquefaciensH47 for making mulberry leaf apocynum venetum leaf enzyme hair care cream Download PDFInfo
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- CN105267135A CN105267135A CN201510575206.1A CN201510575206A CN105267135A CN 105267135 A CN105267135 A CN 105267135A CN 201510575206 A CN201510575206 A CN 201510575206A CN 105267135 A CN105267135 A CN 105267135A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/12—Preparations containing hair conditioners
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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Abstract
The present invention provides a method using BacillusamyloliquefaciensH47 for making mulberry leaf apocynum venetum leaf enzyme hair care cream, the characteristics of the process are that: mulberry leaf and apocynum venetum leaf are used as main raw materials, Bacillus amyloliquefaciens H47 is used as a fermentation strain, the Bacillus amyloliquefaciens H47 is preserved in CCTCC(China Center For Type Culture Collection) on June 24, 2013, the accession number is CCTCC NO: M2013283, the mulberry leaf and apocynum venetum leaf, the Bacillus amyloliquefaciens H47 and the pure water are added and evenly mixed in the volume ratio of 2-5:1-3:7-2 to prepare a mulberry leaf apocynum venetum leaf fermentation raw liquid, a Bacillus amyloliquefaciens H47 and lactic acid bacteria seed liquid is inoculated into the fermentation raw liquid at the temperature of 25 DEG C-35 DEG C according to the proportion of 1-5% for fermenting for 6-14d under the condition of pH value of 5.0-8.0 to obtain a mulberry leaf apocynum venetum leaf enzyme beverage crude product; and large particle residues are preliminarily screened out by coarse filtration with a 20-32 mesh filtration sieve, and small particle residues are further filtered by fine filtration by use of a 80 to 100 mesh filtration sieve to obtain the mulberry leaf apocynum venetum leaf enzyme hair care cream.
Description
Technical field
The present invention relates to the production method of ferment, especially the fermentation process of Folium Mori Folium Cannabis hair ointment, belongs to technical field of bioengineering.
Background technology
Ferment and Japanese, to a kind of call of enzyme (enzyme), were referred to as ferment the initial bio-active products obtained through fermentable afterwards.But because many producers do not note the use of fermentative microorganism and wild functional material, ferment product development and application are subject to its side effect and functional not strong impact.The present invention, by a large amount of screenings and experiment, obtains the ferment manufacture method of a kind of successful, green health.
Folium Mori taste cold in nature bitter in the mouth, sweet, return lung meridian, Liver Channel.There is the effects such as liver heat removing nourishing the liver, dispelling wind and heat pathogens, lung heat clearing, improving eyesight, cure mainly anemopyretic cold, pathogenic wind-warm from the beginning of, fever and headache, perspiration and aversion to wind, cough chest pain, dryness of the lung dry cough without phlegm, dry throat and mouth, wind heat and liver-yang are disturbed, conjunctival congestion and swelling pain.Record according to " Bencao Jingshu ", Folium Mori are sweet so beneficial blood, cold so removing heat from blood, sweetly coldly to meet, therefore the therapeutic method to keep the adverse QI flowing downwards and tonifying YIN, are with can main deficiency of YIN cold and heat and perspiring because of interior-heat.Its property is held concurrently dry, therefore can remove beriberi edema again, and sharp intestine and small intestine, except wind.Respectful through frost Weihe of then holding concurrently, therefore again can improving eyesight and quenching the thirst.After originator blood also, beneficial blood therefore again can long hair, removing heat from blood therefore emesis blood again.Platform carbuncle mouth, stays and wears the palm, and treat soup fire, all refrigerant merit of enriching blood also.Therefore Folium Mori can promote natural on-off cycles of hair growth, and what hair was become is pitch-black beautiful.
Folium Apocyni Veneti sweet in the mouth cool in nature, hardship, return Liver Channel; There is suppressing liver-YANG, the effects such as clearing away heat and promoting diuresis.The merit of the existing suppressing liver-YANG of Folium Apocyni Veneti, has again the effect of the eliminating pathogen in the liver heat clearly, therefore can treat having a dizzy spell that excessive rising of liver-YANG and liver-fire are attacked, irritated insomnia etc.It also has good clearing away heat and promoting diuresis effect, harnesses the river swollen, dysuria and have hot person.This technology with fresh Folium Mori Folium Cannabis for main material, probiotics fermention in application nectar source microorganism prepares ferment, not only the originally function of new fresh mulberry leaf Folium Cannabis will be kept, also the benefit materials of the resisting pathogenic microbes that its probiotic bacteria produces will be given, there is provided the various prebioticses of probiotic bacteria, for the healthy of consumer provides new functional product.
Summary of the invention
The deposit number that the present invention is stored in Wuhan, China Wuhan University China typical culture collection center with on June 24th, 2013 is: the bacillus amyloliquefaciens of the BacillusamyloliquefaciensH47 of CCTCCNO:M2013283 is for fermentation strain.Provide a kind of preparation method that can keep Folium Mori Folium Cannabis ferment nutritive and health protection components to greatest extent, solve in existing ferment production process the pollution that can not ensure without pathogen, fermentation time is long, and the problem such as effectively enzyme activity cannot to be retained for a long time.
For solving the problems of the technologies described above, the technical solution used in the present invention is:
(1) selected material: select the Folium Mori Folium Cannabis that full maturity, natural ferment content are high to be fermentation main material.
(2) process of material: first use tap water Folium Mori Folium Cannabis surface, re-use a century that the century-old still diseases caused by retention of fluid product company limited in Wuxi provides still water clear water wash twice, finally invade steep 1-5 minute by the NaCl solution of 0.9% again, drain for subsequent use.
(3) spawn culture: a century using the century-old still diseases caused by retention of fluid product company limited in Wuxi to provide still water preparation LB culture medium, by former preservation bacterial strain activation, cultivates the bacterium liquid obtained for 48 hours for subsequent use;
(4) inoculation fermentation: according to volume ratio Folium Mori Folium Cannabis: green molasses: pure water is 2-5: 1-3: 7-2 add rear mix homogeneously and namely obtain Folium Mori Folium Cannabis fermenation raw liquid, according to the ratio of 1-5%, nectar source bacterium and lactobacillus solution are accessed fermenation raw liquid, each seed liquor concentration is 10
6cFU/ml, fermentation 6-14d, pH value 5.0-8.0, temperature 25 DEG C-35 DEG C
(5) post-processed makes: coarse filtration, tentatively screens out bulky grain residue, avoids blade to put for a long time easily causing becoming sour, filtercondition: sieves number is 20 ~ 32 orders.Finly filtration: filter small particle residue further, makes liquid state present limpider state, filtercondition: sieves number is 80 ~ 100 orders, both natural Folium Mori Folium Cannabis ferment beverage.
In step (1), the preparation method of Folium Mori Folium Cannabis ferment raw material comprises the steps:
(1.1) instrument used, utensil all want sterilizing, without greasy.
(1.2) Folium Mori hemp leaf beetle pure water is cleaned, then drain with the saline soak of pure water or 0.9%.
(1.3) again Folium Mori Folium Cannabis is placed in sterilized container, after add the century-old Shang Shui that the century-old still diseases caused by retention of fluid product company limited in green molasses and Wuxi provides, wherein volume ratio Folium Mori Folium Cannabis in fermenation raw liquid: green molasses: pure water=2 ~ 5: 1 ~ 3: 7 ~ 2, obtains Folium Mori Folium Cannabis fermenation raw liquid.
Beneficial effect of the present invention:
1. effectively reduce the loss of ferment nutritional labeling under low temperature, remain enzyme activity to greatest extent.
2. having accessed dominant strain inhibits other miscellaneous bacterias to grow, and makes constant product quality.
3. the total time of fermentation is about 10 days, and the time is short to be avoided polyoxygenated, was conducive to the ferment material keeping Folium Mori Folium Cannabis itself to contain.
Detailed description of the invention
The following stated is only preferred embodiments of the present invention, not in order to limit the present invention, all within thinking of the present invention and principle, and any amendment made, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Embodiment 1
To ferment the method for Folium Mori Folium Cannabis ferment, comprise the following steps:
(1) fresh Folium Mori Folium Cannabis is selected to make each 500g for fermentation primary raw material.
(2) pure water clear water washes twice, and finally soak by the NaCl solution of 0.9%, cold sterilization, then drains again, and load in the cleaned container of pure water, probably account for 30% of volume, adding the green molasses accounting for total liquid volume 10% is that fermenation raw liquid is for subsequent use.
(3) prepare LB culture medium, by former preservation bacterial strain activation, cultivate the zymocyte liquid obtained for about 48 hours for subsequent use;
(4) according to inoculative proportion 5%, nectar source bacterium, lactobacillus are got access fermenation raw liquid respectively, fermentation 7d, pH value 7.0, temperature 25 DEG C.
(5) first carry out coarse filtration, sieves number is 20 ~ 32 orders.Then finly filtration, sieves number, for being 80 ~ 100 orders, obtains the natural Folium Mori Folium Cannabis ferment of clarification.
Claims (5)
1. make a method for Folium Mori Folium Cannabis ferment hair ointment of nectar source bacterium, be characterized in fresh Folium Mori Folium Cannabis for main material, application nectar source bacterium fermentation is for ferment hair ointment, and the method comprises the following steps:
(1) selected material: select fresh Folium Mori Folium Cannabis for fermentation main material;
(2) process of material: first use tap water Folium Mori Folium Cannabis surface, re-use a century that the century-old still diseases caused by retention of fluid product company limited in Wuxi provides still water clear water wash twice, finally soak 1-5 minute by the NaCl solution of 0.9% again, make after draining starch for subsequent use;
(3) spawn culture: a century using the century-old still diseases caused by retention of fluid product company limited in Wuxi to provide still water preparation LB culture medium, by former preservation bacterial strain activation, cultivates the seed liquor for subsequent use obtained for about 48 hours;
(4) inoculation fermentation: according to volume ratio Folium Mori Folium Cannabis: green molasses: pure water is 2-5: 1-3: 7-2 add rear mix homogeneously and namely obtain Folium Mori Folium Cannabis fermenation raw liquid, according to the ratio of 1-5% by nectar source bacterium seed liquor access fermenation raw liquid, seed liquor concentration is 10
6cFU/ml, fermentation 6-14d, pH value 5.0-8.0, temperature 25 DEG C-35 DEG C;
(5) post-processed makes: coarse filtration: tentatively screen out bulky grain residue, and avoid blade to put for a long time and form precipitation, sieves number is 20-32 order; Finly filtration: filter small particle residue further, makes liquid state present limpider state, and sieves number is 80-100 order, namely obtains Folium Mori Folium Cannabis ferment hair ointment.
2. a kind of nectar source according to claim 1 bacterium makes the method for Folium Mori Folium Cannabis ferment hair ointment, and it is characterized in that: Folium Mori refer to the blade of Moraceae Morus Mulberry kind, Folium Cannabis refers to that Folium Cannabis is the blade of apocynaceae plant Herba Apocyni veneti.
3. a kind of nectar source according to claim 1 bacterium makes the method for Folium Mori Folium Cannabis ferment hair ointment, it is characterized in that: nectar source bacterium refers to that the deposit number being stored in Wuhan University's China typical culture collection center on June 24th, 2013 is the Bacillus amyloliquefaciens strain of the BacillusamyloliquefaciensH47 of CCTCCNO:M2013283, and addition is 1%-5%.
4. a kind of nectar source according to claim 1 bacterium makes the method for Folium Mori Folium Cannabis ferment hair ointment, it is characterized in that: at pH value 5.0-8.0, the condition bottom fermentation incubation time 6-14d of temperature 25 DEG C-35 DEG C.
5. a kind of nectar source according to claim 1 bacterium makes the method for Folium Mori Folium Cannabis ferment hair ointment, it is characterized in that: fermentation finished product is through coarse filtration and finly filtration two steps, and coarse filtration sieves number is 20-32 order; Finly filtration sieves number is 80-100 order.
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| CN201510575206.1A CN105267135A (en) | 2015-09-11 | 2015-09-11 | Method using BacillusamyloliquefaciensH47 for making mulberry leaf apocynum venetum leaf enzyme hair care cream |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106616973A (en) * | 2016-11-10 | 2017-05-10 | 江苏丹枫神源生物科技有限公司 | Preparation method of acer truncatum pterodium living bacteria enzymes stock solution |
| CN108653073A (en) * | 2018-05-04 | 2018-10-16 | 江苏欧亚立日化有限公司 | A kind of long-acting moisturizing shampoo of softness and preparation method thereof |
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| WO2010108781A1 (en) * | 2009-03-23 | 2010-09-30 | Wacker Chemie Ag | Process for making stable high viscosity amino silicone emulsion |
| CN103451128A (en) * | 2013-07-12 | 2013-12-18 | 江南大学 | Bacillus amyloliquefaciens and application thereof in preventing and controlling peach bleeding disease |
| CN103876155A (en) * | 2014-04-01 | 2014-06-25 | 田雷 | Composite plant enzyme containing probiotics and application of composite plant enzyme |
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2015
- 2015-09-11 CN CN201510575206.1A patent/CN105267135A/en active Pending
Patent Citations (3)
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| WO2010108781A1 (en) * | 2009-03-23 | 2010-09-30 | Wacker Chemie Ag | Process for making stable high viscosity amino silicone emulsion |
| CN103451128A (en) * | 2013-07-12 | 2013-12-18 | 江南大学 | Bacillus amyloliquefaciens and application thereof in preventing and controlling peach bleeding disease |
| CN103876155A (en) * | 2014-04-01 | 2014-06-25 | 田雷 | Composite plant enzyme containing probiotics and application of composite plant enzyme |
Non-Patent Citations (1)
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| 杜杰慧: "《美容我要纯天然》", 31 March 2013, 中国妇女出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106616973A (en) * | 2016-11-10 | 2017-05-10 | 江苏丹枫神源生物科技有限公司 | Preparation method of acer truncatum pterodium living bacteria enzymes stock solution |
| CN108653073A (en) * | 2018-05-04 | 2018-10-16 | 江苏欧亚立日化有限公司 | A kind of long-acting moisturizing shampoo of softness and preparation method thereof |
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Application publication date: 20160127 |