A kind of is the treatment of purpose Isolated Tooth and the method for storing to recycle
Technical field
The present invention relates to a kind of processing method of autologous tooth, it is more particularly to a kind of to recycle for the treatment of purpose Isolated Tooth and
The method of storage.
Background technology
The labyrinth that tooth is made up of organic principles such as the inorganic constituents and collagen based on phosphorite crystal,
Composition with bone tissue is closely similar.Domestic and international numerous studies prove that tooth bud is sent out to maxilla and related cartilage, the growth of nerve
The source educated is cranial neural crest.The 96% of ripe enamel weight is inorganic matter, and 4% is organic matter and water;And in dentine
Both ratios are 65:35;Its ratio is 45~50 in cementum:50~55;Alveolar bone is then by 65% inorganic matter and 35%
Organic matter and water are constituted.So, the constituent of tooth, especially dentine, the composition with bone tissue are closely similar.
Early in 1967, there is researcher to find to fill rabbit off ore deposit dentine and mineralising dentine and ground one's teeth in sleep extraction tooth in rabbit
At groove nest, new bone was produced at 4 weeks and 8~12 weeks respectively, it was demonstrated that dentine has induction bone and a chondrogenetic effect.21 generation
Record just, scholars confirm tooth by having induction alveolar bone new bone formation after treatment, preparation by animal experiment study
Ability.In recent years, the tooth induction clinically pulled out and discarded using patient promotes jawbone, maxillary sinus or extraction
Knitting and new bone formation at nest, with preferable clinical therapeutic efficacy.
According to the difference of patient itself sufferer situation, some patientss are carried out to teeth in vitro immediately after tooth is pulled out
Prepared by treatment, reuse, and carries out the treatment such as bone grafting.Another part patient is then needed after the several years using having pulled out
Teeth in vitro is treated again, and the teeth in vitro of this some patients then needs to be stored in tooth bank, and ensures its tooth
Physicalchemical structure stabilization and aseptic guarantee.
Not yet there is a set of standardized medical system the country and pulls out tooth archives, treatment for setting up patient, stores at present
And safety detection.As the tooth pulled out using patient itself carries out pushing away for clinical treatment as itself bone grafting material
Extensively, tooth bank set up can not only systematization managing patient tooth record achieve, it is often more important that after to pulling out
The treatment and storage of teeth in vitro, ensure that patient needs to reuse tooth when being treated, the physicalchemical structure of tooth with
And the function of induction bon e formation bone remoulding keeps its stabilization, can be used for treating again, it is ensured that the success rate of operation.
The content of the invention
The present invention is to overcome above-mentioned defect in the prior art, there is provided it is a kind of to recycle for the treatment of purpose Isolated Tooth and
The method of storage.
A kind of is the treatment of purpose Isolated Tooth and the method for storing to recycle, and is carried out in accordance with the following steps:
(1) patient's extraction archives are set up, the relevant information of tooth is recorded and is stored;
(2) tooth of the patient without treatment or reserve value is pulled out;
(3) tooth that will be pulled up soaks 0.5-2min in being put into protection liquid;
(4) the dental caries bad part of teeth in vitro, remaining soft tissue and dental pulp are removed, and ultrasonic vibration is washed, and is left normal
Enamel, dentine and cementum;
(5) by tooth storage container, storage solutions or frozen stock solution, Cord blood are added.
The extraction archives include patient's name, and sex pulls out the tooth position of tooth, date of birth, the time performed the operation, connection
It is mode, auxiliary examination result and X-ray film.
The protection liquid is made up of the component of following parts by weight:Penicillin 0.1-0.5 parts, metronidazole 0.01-0.05 parts,
0.2-0.5 parts of the acetoxyl group -9- carbonyls Herba Lycopi's ketone of 2- deoxidations -2, hydroxymethyl cellulose 10-15 parts, riboflavin 0.1-0.2 parts, nothing
900-1000 parts of bacterium water.Above-mentioned protection liquid has the effect of cleaning, sterilization for subject ex vivo's tooth, it is ensured that using tooth enamel
The tissue integrity and aseptic of matter, dentine and cementum.
The step of dental caries bad part of the removal teeth in vitro, remaining soft tissue and dental pulp, is manual using dental high-speed cell phone
Curettage instrument is used in combination.
The step (5) if in add storage solutions, within preserving 6 years under the conditions of 0-18 DEG C;If adding frozen stock solution,
Within then preserving 30 years.
The component composition of the following parts by weight of storage solutions:Poly-D-lysine 1-5 parts, dextran 2-4 parts, glycerine
5-15 parts, hyclone 5-10 parts, trehalose 1-5 parts, deionized water 25-85 parts.
The frozen stock solution is liquid nitrogen.
Compared with prior art, the present invention has the advantages that:The method of the present invention is low to subject ex vivo's tooth
Temperature storage, it is ensured that the stability of its tooth physicalchemical structure, retains the ability of original promotion knitting and bone remoulding, it is ensured that
Patient carried out for example carrying out bone grafting etc. as bone grafting material controlling curative effect in treatment using pulling out tooth in 10 years
Fruit and success rate of operation.Tooth stocking system of the invention, Collection and conservation patient itself pulls out tooth, is used on suitable opportunity
Patient itself treats again.This theory by discarded medical resource cycling and reutilization, meets the development of present medical idea.This
Invention is perspective with certain science, and the tooth of storage carries out bone by being substituted for other bone grafting materials after preparation
Transplantation, and with other bone grafting materials without advantage.With the development of medical technology, autologous tooth in scientific research and
Clinical application will all the more extensively, and the effect of tooth banking system also will be more significantly.Additionally, the temperature stored in the experiment
If degree room temperature, storage time most long is 3 years.The present invention uses low-temperature storage, with its storage time of the reduction of storage temperature
Can greatly prolong.If taking liquid nitrogen cryogenics storing method, the storage time that patient pulls out tooth can extend to more than 10 years.Therefore,
According to the state of an illness of patient and self-demand can pull out the personalized storage of tooth.
Specific embodiment
Specific embodiment of the invention is described in detail with reference to specific embodiment, it is to be understood that of the invention
Protection domain do not limited by specific embodiment.
Embodiment 1
Wang, 34 years old, needs to pull out, because Dental implantion Bone mineral change need to go increase bone amount after the longitudinal direction jackknifing of upper right front tooth wound
Bone grafting.
In accordance with the following steps for it sets up dental procedure and storage:
(1) patient's extraction archives are set up, the basic personal considerations' information of collection patient includes patient's name, sex, date of birth
Phase and contact method, carry out Orhopantomography and CBCT is checked, it is determined that treatment plan after pulling out the tooth position of tooth and having tooth pulled out.
Explanation patient needs to pull out tooth, and carries out bone grafting using the tooth pulled out, and obtains patient's agreement, signs and agrees to
Book.
(2) pull out and suffer from tooth, the tooth that will be pulled up soaks 1min in being put into protection liquid;The protection liquid is by following parts by weight
Component composition:0.3 part of penicillin, 0.04 part of metronidazole, 0.3 part of the acetoxyl group -9- carbonyl Herba Lycopi ketone of 2- deoxidations -2, methylol
12 parts of cellulose, 0.15 part of riboflavin, 980 parts of sterilized water.
(3) with dental high-speed cell phone, the removal of manual curettage instrument pull out tooth dental caries are bad, remaining soft tissue, remove dental pulp,
And ultrasonic vibration is washed, and only leaves normal enamel, dentine and cementum.
(4) by tooth storage container, storage solutions are added;The component composition of the following parts by weight of storage solutions:
3 parts of poly-D-lysine, 3 parts of dextran, 8 parts of glycerine, 6 parts of hyclone, 2 parts of trehalose, 55 parts of deionized water.
Patient carries out the tooth of bone grafting, X-ray film display patient bone graft area and surrounding using the autologous tooth of storage 3 months
Groove bone density is close, bone graft area sclerotin is uniform, it is ensured that tooth implantation is smoothed out.
Embodiment 2
Zhang, 38 years old, upper right molar molar need pull out, because Dental implantion Bone mineral change need to go increase bone amount bone collection
Art.
In accordance with the following steps for it sets up dental procedure and storage:
(1) patient's extraction archives are set up, the basic personal considerations' information of collection patient includes patient's name, sex, date of birth
Phase and contact method, carry out Orhopantomography and CBCT is checked, it is determined that treatment plan after pulling out the tooth position of tooth and having tooth pulled out.
Explanation patient needs to pull out tooth, and carries out bone grafting using the tooth pulled out, and obtains patient's agreement, signs and agrees to
Book.
(2) pull out and suffer from tooth, the tooth that will be pulled up soaks 1min in being put into protection liquid;The protection liquid is by following parts by weight
Component composition:0.1 part of penicillin, 0.05 part of metronidazole, 0.5 part of the acetoxyl group -9- carbonyl Herba Lycopi ketone of 2- deoxidations -2, methylol
10 parts of cellulose, 0.2 part of riboflavin, 900 parts of sterilized water.
(3) with dental high-speed cell phone, the removal of manual curettage instrument pull out tooth dental caries are bad, remaining soft tissue, remove dental pulp,
And ultrasonic vibration is washed, and only leaves normal enamel, dentine and cementum.
(4) by tooth storage container, storage solutions are added;The component composition of the following parts by weight of storage solutions:
5 parts of poly-D-lysine, 2 parts of dextran, 5 parts of glycerine, 10 parts of hyclone, 1 part of trehalose, 85 parts of deionized water.
Patient carries out the tooth of bone grafting, X-ray film display patient bone graft area and surrounding using the autologous tooth of storage 3 months
Groove bone density is close, bone graft area sclerotin is uniform, it is ensured that tooth implantation is smoothed out.
Embodiment 3
Zhang, 30 years old, molar molar in upper left needed to pull out, because Dental implantion Bone mineral change need to go the bone collection for increasing bone amount
Art.
In accordance with the following steps for it sets up dental procedure and storage:
(1) patient's extraction archives are set up, the basic personal considerations' information of collection patient includes patient's name, sex, date of birth
Phase and contact method, carry out Orhopantomography and CBCT is checked, it is determined that treatment plan after pulling out the tooth position of tooth and having tooth pulled out.
Explanation patient needs to pull out tooth, and carries out bone grafting using the tooth pulled out, and obtains patient's agreement, signs and agrees to
Book.
(2) pull out and suffer from tooth, the tooth that will be pulled up soaks 1min in being put into protection liquid;The protection liquid is by following parts by weight
Component composition:0.1 part of penicillin, 0.05 part of metronidazole, 0.5 part of the acetoxyl group -9- carbonyl Herba Lycopi ketone of 2- deoxidations -2, methylol
10 parts of cellulose, 0.2 part of riboflavin, 950 parts of sterilized water.
(3) with dental high-speed cell phone, the removal of manual curettage instrument pull out tooth dental caries are bad, remaining soft tissue, remove dental pulp,
And ultrasonic vibration is washed, and only leaves normal enamel, dentine and cementum.
(4) by tooth storage container, liquid nitrogen storage is added.
Patient carries out the alveolus of bone grafting, X-ray film display patient bone graft area and surrounding using the autologous tooth of storage 3 years
Bone density is close, bone graft area sclerotin is uniform, it is ensured that tooth implantation is smoothed out.
Embodiment 4
Choose 15 fresh patients stored in tooth and 15 tooth banks that pull out and pull out tooth (using embodiment 1
Treatment and storage method, storage time be 3 years) be analyzed, that is, after preparing as after bone grafting material to its physics
Chemical composition and structure are detected.Teeth in vitro calcium-phosphorus ratio and organic components in result display tooth bank with it is new
It is fresh to pull out tooth there was no significant difference, and sample dentinal tubule in scanning electron microscopy analysis shows tooth bank it is clog-free,
Sexually revised without constriction, collagenous fibres are without gelation without the release and new bone formation battalion for being denatured-showing rush bone remoulding growth factor
The maintenance of the abilities such as foster supply.Bacteria Detection structure shows teeth in vitro its bacterium value of storage within normal range (NR).On
State testing result to show, teeth in vitro its physicalchemical structure stabilization stored in tooth bank, still with stronger promotion bone
Healing and the ability of osteanagenesis.
Embodiment 5
Choose 15 fresh patients stored in tooth and 15 tooth banks that pull out and pull out tooth (using embodiment 3
Treatment and storage method, storage time be 8 years) be analyzed, that is, after preparing as after bone grafting material to its physics
Chemical composition and structure are detected.Teeth in vitro calcium-phosphorus ratio and organic components in result display tooth bank with it is new
It is fresh to pull out tooth there was no significant difference, and sample dentinal tubule in scanning electron microscopy analysis shows tooth bank it is clog-free,
Sexually revised without constriction, collagenous fibres are without gelation without the release and new bone formation battalion for being denatured-showing rush bone remoulding growth factor
The maintenance of the abilities such as foster supply.Bacteria Detection structure shows teeth in vitro its bacterium value of storage within normal range (NR).On
State testing result to show, teeth in vitro its physicalchemical structure stabilization stored in tooth bank, still with stronger promotion bone
Healing and the ability of osteanagenesis.
Comparative example 1
Money, 34 years old, needs to pull out, because Dental implantion Bone mineral change need to go increase bone amount after the longitudinal direction jackknifing of upper right front tooth wound
Bone grafting.
In accordance with the following steps for it sets up dental procedure and storage:
(1) patient's extraction archives are set up, the basic personal considerations' information of collection patient includes patient's name, sex, date of birth
Phase and contact method, carry out Orhopantomography and CBCT is checked, it is determined that treatment plan after pulling out the tooth position of tooth and having tooth pulled out.
Explanation patient needs to pull out tooth, and carries out bone grafting using the tooth pulled out, and obtains patient's agreement, signs and agrees to
Book.
(2) pull out and suffer from tooth, the tooth that will be pulled up soaks 1min in being put into protection liquid;The protection liquid is by following parts by weight
Component composition:0.3 part of penicillin, 0.04 part of metronidazole, 12 parts of hydroxymethyl cellulose, 0.15 part of riboflavin, sterilized water 980
Part.
(3) with dental high-speed cell phone, the removal of manual curettage instrument pull out tooth dental caries are bad, remaining soft tissue, remove dental pulp,
And ultrasonic vibration is washed, and only leaves normal enamel, dentine and cementum.
(4) by tooth storage container, storage solutions are added;The component composition of the following parts by weight of storage solutions:
3 parts of poly-D-lysine, 3 parts of dextran, 8 parts of glycerine, 6 parts of hyclone, 2 parts of trehalose, 55 parts of deionized water.
Patient carries out the tooth of bone grafting, X-ray film display patient bone graft area and surrounding using the autologous tooth of storage 3 months
Groove bone density is variant, and bone graft area sclerotin is uniform, and alveolus is subject to bacterium infection, by sterilizing again, it is ensured that tooth implantation
Carry out.
Comparative example 2
Wang, 30 years old, molar molar in upper left needed to pull out, because Dental implantion Bone mineral change need to go the bone collection for increasing bone amount
Art.
In accordance with the following steps for it sets up dental procedure and storage:
(1) patient's extraction archives are set up, the basic personal considerations' information of collection patient includes patient's name, sex, date of birth
Phase and contact method, carry out Orhopantomography and CBCT is checked, it is determined that treatment plan after pulling out the tooth position of tooth and having tooth pulled out.
Explanation patient needs to pull out tooth, and carries out bone grafting using the tooth pulled out, and obtains patient's agreement, signs and agrees to
Book.
(2) pull out and suffer from tooth, the tooth that will be pulled up soaks 1min in being put into protection liquid;The protection liquid is by following parts by weight
Component composition:0.1 part of penicillin, 0.05 part of metronidazole, 0.5 part of the acetoxyl group -9- carbonyl Herba Lycopi ketone of 2- deoxidations -2, riboflavin
0.2 part, 950 parts of sterilized water.
(3) with dental high-speed cell phone, the removal of manual curettage instrument pull out tooth dental caries are bad, remaining soft tissue, remove dental pulp,
And ultrasonic vibration is washed, and only leaves normal enamel, dentine and cementum.
(4) by tooth storage container, storage solutions are added;The component composition of the following parts by weight of storage solutions:
3 parts of poly-D-lysine, 3 parts of dextran, 8 parts of glycerine, 6 parts of hyclone, 2 parts of trehalose, 55 parts of deionized water.
Patient carries out the alveolus of bone grafting, X-ray film display patient bone graft area and surrounding using the autologous tooth of storage 3 years
Bone density is variant, and bone graft area sclerotin is uneven, it is impossible to ensure the carrying out of tooth implantation.
Comparative example 3
Wang, 38 years old, upper right molar molar need pull out, because Dental implantion Bone mineral change need to go increase bone amount bone collection
Art.
In accordance with the following steps for it sets up dental procedure and storage:
(1) patient's extraction archives are set up, the basic personal considerations' information of collection patient includes patient's name, sex, date of birth
Phase and contact method, carry out Orhopantomography and CBCT is checked, it is determined that treatment plan after pulling out the tooth position of tooth and having tooth pulled out.
Explanation patient needs to pull out tooth, and carries out bone grafting using the tooth pulled out, and obtains patient's agreement, signs and agrees to
Book.
(2) pull out and suffer from tooth, the dental caries for pulling out tooth with dental high-speed cell phone, the removal of manual curettage instrument are bad, remaining soft tissue,
Removal dental pulp, and ultrasonic vibration washing, only leave normal enamel, dentine and cementum.
(3) by tooth storage container, storage solutions are added;The component composition of the following parts by weight of storage solutions:
5 parts of poly-D-lysine, 2 parts of dextran, 5 parts of glycerine, 10 parts of hyclone, 1 part of trehalose, 85 parts of deionized water.
Patient carries out the tooth of bone grafting, X-ray film display patient bone graft area and surrounding using the autologous tooth of storage 3 months
Groove bone density is differed greatly, and bone graft area sclerotin is uneven, and alveolar bone has largely infected bacterium, and tooth implantation cannot be carried out.
Comparative example 4
Leaf, 38 years old, upper right molar molar need pull out, because Dental implantion Bone mineral change need to go increase bone amount bone collection
Art.
In accordance with the following steps for it sets up dental procedure and storage:
(1) patient's extraction archives are set up, the basic personal considerations' information of collection patient includes patient's name, sex, date of birth
Phase and contact method, carry out Orhopantomography and CBCT is checked, it is determined that treatment plan after pulling out the tooth position of tooth and having tooth pulled out.
Explanation patient needs to pull out tooth, and carries out bone grafting using the tooth pulled out, and obtains patient's agreement, signs and agrees to
Book.
(2) pull out and suffer from tooth, the tooth that will be pulled up soaks 1min in being put into protection liquid;The protection liquid is by following parts by weight
Component composition:0.1 part of penicillin, 0.05 part of metronidazole, 0.5 part of the acetoxyl group -9- carbonyl Herba Lycopi ketone of 2- deoxidations -2, methylol
10 parts of cellulose, 0.2 part of riboflavin, 900 parts of sterilized water.
(3) with dental high-speed cell phone, the removal of manual curettage instrument pull out tooth dental caries are bad, remaining soft tissue, remove dental pulp,
And ultrasonic vibration is washed, and only leaves normal enamel, dentine and cementum.
(4) submergence of 75% medicinal alcohol will in tooth storage container, be added.
Patient carries out the tooth of bone grafting, X-ray film display patient bone graft area and surrounding using the autologous tooth of storage 3 months
Groove bone density is close, and bone graft area sclerotin is slightly uneven, and tooth implantation can be carried out, and planting effect is poor.
Comparative example 5
Wang, 38 years old, upper right molar molar need pull out, because Dental implantion Bone mineral change need to go increase bone amount bone collection
Art.
In accordance with the following steps for it sets up dental procedure and storage:
(1) patient's extraction archives are set up, the basic personal considerations' information of collection patient includes patient's name, sex, date of birth
Phase and contact method, carry out Orhopantomography and CBCT is checked, it is determined that treatment plan after pulling out the tooth position of tooth and having tooth pulled out.
Explanation patient needs to pull out tooth, and carries out bone grafting using the tooth pulled out, and obtains patient's agreement, signs and agrees to
Book.
(2) pull out and suffer from tooth, the tooth that will be pulled up soaks 1min in being put into protection liquid;The protection liquid is by following parts by weight
Component composition:0.1 part of penicillin, 0.05 part of metronidazole, 0.5 part of the acetoxyl group -9- carbonyl Herba Lycopi ketone of 2- deoxidations -2, methylol
10 parts of cellulose, 0.2 part of riboflavin, 900 parts of sterilized water.
(3) with dental high-speed cell phone, the removal of manual curettage instrument pull out tooth dental caries are bad, remaining soft tissue, remove dental pulp,
And ultrasonic vibration is washed, and only leaves normal enamel, dentine and cementum.
(4) submergence of 75% medicinal alcohol will in tooth storage container, be added.
Patient carries out the alveolus of bone grafting, X-ray film display patient bone graft area and surrounding using the autologous tooth of storage 3 years
Bone density is not close, and bone graft area sclerotin is uneven, and tooth implantation cannot be carried out.
The above, the only present invention preferably specific embodiment, but protection scope of the present invention is not limited thereto,
Any one skilled in the art in the technical scope of present disclosure, technology according to the present invention scheme and its
Inventive concept is subject to equivalent or change, should all be included within the scope of the present invention.