CN105251006B - Purposes of the TLR3 inhibitor in the drug for preparing treatment cocaine habituation - Google Patents

Abstract

The invention discloses purposes of the TLR3 inhibitor in the drug for preparing treatment dependence producing drug habituation.The invention also discloses a kind of drugs for treating cocaine habituation, it is using TLR3 inhibitor as active constituent, in addition the preparation that pharmaceutically acceptable auxiliary material or complementary ingredient are prepared.TLR3 inhibitor of the present invention can effectively weaken the dependence and craving to cocaine, weaken the locomotor sensitivity effect of cocaine induction, can treat cocaine habituation, potential applicability in clinical practice is good.

Description

Purposes of the TLR3 inhibitor in the drug for preparing treatment cocaine habituation

Technical field

The present invention relates to purposes of the TLR3 inhibitor in the drug for preparing treatment cocaine habituation.

Background technology

Cocaine (Cocaine) is also known as cocaine, the entitled benzoyl-methyl-ecgonine (methyl of chemistry Benzoylecgonine), general white lenticular, it is odorless, bitter and it is numb, be used for local anaesthesia earliest and treat asthma, It is strongest natural central stimulant, causes to abuse because of the excitation of its Central nervous system, as generation from 1985 One of main drugs of criticality.

Cocaine habituation is a kind of chronic recurrent brain diseases, belongs to pharmacological dependence (drug dependence) class disease Disease, cocaine habituation can cause the Changes of Plasticity of brain structure and function, related brain areas to include nucleus accumbens septi, corpus straitum, forehead Cortex, hippocampus and ventral tegmental area, health are also disorderly by various harm, including mental deterioration, personality defect, intelligence function Disorderly, concurrent corresponding infection complication and drug addict seek and are induced using drugs various delinquent work at all adventures It is dynamic.

The main feature of cocaine habituation is shown as even if patient after knowing the serious consequence of medication, still forces sex cords Take and use with meet desire, to drug seek and ask for it is out of hand, lose interest to things, Drug addiction it is very deep It carves, after the withdrawal and treatment several years, contacts stimulation related with habituation (such as poison friend, environment related with medication in the past Deng) all may induce relapse.

It is very big in view of cocaine habituation harm, suitable therapy target and drug are found, treatment cocaine habituation is compeled in eyebrow Eyelash.

Invention content

To solve the above-mentioned problems, the present invention provides the drugs of a kind for the treatment of cocaine habituation.

TLR3：Toll-like receptor 3.

TLR3 inhibitor：Inhibit the activity of Toll-like receptor 3 or the substance of expression.

Present invention firstly provides purposes of the TLR3 inhibitor in the drug for preparing treatment cocaine habituation.

Preferably, the drug of the treatment cocaine habituation is the drug for reducing pIKB α and NF-kB expressions.

Preferably, the TLR3 inhibitor is compound I, and structural formula is as follows：

Entitled (R) -2- (3-Chloro-6- fluorobenzo[b]thiophene-2-carboxamido)-3-phenylpropanoic acid。

The present invention also provides a kind of drugs for treating cocaine habituation, it is added using TLR3 inhibitor as active constituent The preparation that upper pharmaceutically acceptable auxiliary material or complementary ingredient are prepared.

Preferably, the drug of the treatment cocaine habituation is the drug for reducing pIKB α and NF-kB expressions.

Preferably, the TLR3 inhibitor is compound I, and structural formula is as follows：

Preferably, the preparation is ejection preparation.

TLR3 inhibitor can effectively weaken the dependence and craving to cocaine, weaken the locomotor sensitivity effect of cocaine induction It answers, cocaine habituation can be treated, potential applicability in clinical practice is good.

The specific implementation mode of form by the following examples makees further specifically the above of the present invention It is bright.But the range that this should not be interpreted as to the above-mentioned theme of the present invention is only limitted to embodiment below.It is all to be wanted based on right of the present invention The technology that the content that secretary carries is realized is asked to all belong to the scope of the present invention.

Description of the drawings

Fig. 1 pReceiver-Lv201 carrier figures

Fig. 2 mouse Conditioned place preference casees

Fig. 3 self administration systems.

Fig. 4 spontaneous activity devices.

Fig. 5 nucleus accumbens septi anatomical atlas

The Conditioned place halo effect of Fig. 6 various dose cocaines.*p<0.05, there is statistics poor compared with the control group It is different.20mg/kg：Cocaine 20mg groups；10mg/kg：Cocaine 10mg groups；3mg/kg：Cocaine 3mg/kg groups；Sa:Physiology salt Water control group

The cocaine CPP behaviouristics effects of Fig. 7 TLR3 knock out mice, MyD88 knock out mice.Cocaine (20mg/kg) gives genotype mice CPP effect figures.*p<0.05, there is significant difference compared with the control group.WT：It is wild Type mouse group；TLR3KO：TLR3 knock out mice groups；MyD88KO：MyD88KO knock out mice groups.

The cocaine CPP behaviouristics effects of Fig. 8 TLR3 knock out mice, MyD88 knock out mice.Cocaine (10mg/kg) gives genotype mice CPP effect figure figures.*p<0.05, there is significant difference compared with the control group.WT：It is wild Raw type mouse group；TLR3KO：TLR3 knock out mice groups；MyD88KO：MyD88 knock out mice groups.

The cocaine CPP behaviouristics effects of Fig. 9 TLR3 knock out mice, MyD88 knock out mice.Cocaine (3mg/kg) gives genotype mice CPP effect figures.*p<0.05, there is significant difference compared with the control group.WT：It is wild Type mouse group；TLR3KO：TLR3 knock out mice groups；MyD88KO：MyD88 knock out mice groups.

Figure 10 TLR3 knock out mice and wild-type mice spontaneous activity.Cocaine (20mg/kg) is given wild respectively Type (WT) is organized and TLR3KO groups, 9 time point locomotor activations and baseline ratio after administration.*p<0.05, with control group ratio Relatively there is significant difference.TLR3KO：TLR3 knock out mice groups；WT：Wild-type mice group.

Figure 11 TLR3 knock out mice and wild-type mice spontaneous activity.Cocaine (10mg/kg) is given wild respectively Type (WT) is organized and TLR3KO groups, 9 time point locomotor activations and baseline ratio after administration.*p<0.05, with control group ratio Relatively there is significant difference.TLR3KO：TLR3 knock out mice groups；WT：Wild-type mice group.

Figure 12 TLR3 knock out mice and wild-type mice spontaneous activity.Cocaine (3mg/kg) is given wild respectively Type (WT) is organized and TLR3KO groups, 9 time point locomotor activations and baseline ratio after administration.*p<0.05, with control group ratio Relatively there is significant difference.TLR3KO：TLR3 knock out mice groups；WT：Wild-type mice group.

Figure 13 TLR3 knock out mice and wild-type mice self administration cocaine nose touch number and compare figure.*p< 0.05, there is significant difference compared with the control group.TLR3KO-CO Active：The effective nose of TLR3 knock out mice cocaines touches Number；TLR3KO-CO inactive：The invalid nose of TLR3 knock out mice cocaines touches number；WT-CO Active：It is wild The effective nose of type mouse cocaine touches number；WT-CO inactive：The invalid nose of wild-type mice cocaine touches number.

Figure 14 TLR3 knock out mice and wild-type mice self administration physiological saline nose touch number.*p<0.05, with Control group relatively has significant difference.TLR3KO-Saline Active：The effective nose of TLR3 knock out mice physiological saline touches Number；TLR3KO-Saline Inactive：The invalid nose of TLR3 knock out mice physiological saline touches number；WT-Saline Active：The effective nose of wild-type mice physiological saline touches number；WT-Saline Inactive：Wild-type mice physiological saline without It imitates nose and touches number.

Figure 15 TLR3 knock out mice and wild-type mice self administration cocaine intake compare figure.*p<0.05, There is significant difference compared with the control group.TLR3KO：TLR3 knock out mice groups；WT：Wild-type mice group.

Figure 16 nucleus accumbens septi locating injection slow virus carrier figures

It is inclined that Figure 17 TLR3 knock out mice intracerebral injections TLR3 expression slow virus carriers restore cocaine Conditioned place Good effect.*p<0.05, there is significant difference compared with the control group.LV-TLR3+CO：TLR3 slow virus carrier cocaine groups；LV- Mock+CO：TLR3 slow virus compares cocaine group；LV-TLR3+Sa：TLR3 slow virus carrier physiological saline groups；LV-Mock+ Sa：TLR3 slow virus carrier cocaine groups.

The spontaneous activity of Figure 18 TLR3 knock out mice intracerebral injections TLR3 expression slow virus carrier cocaine (activity away from From).*p<0.05, there is significant difference compared with the control group.LV-TLR3+CO：TLR3 slow virus carrier cocaine groups；LV- Mock+CO：TLR3 slow virus compares cocaine group；LV-TLR3+SA：TLR3 slow virus carrier physiological saline groups；LV-Mock+ SA：TLR3 slow virus carrier cocaine groups.

Figure 19 TLR3 knock out mice intracerebral injections TLR3 expression slow virus carriers enhance cocaine locomotor sensitivity effect (with baseline ratio).*p<0.05, there is significant difference compared with the control group.LV-TLR3+CO：TLR3 slow virus carrier cocaines Group；LV-Mock+CO：TLR3 slow virus compares cocaine group；LV-TLR3+SA：TLR3 slow virus carrier physiological saline groups；LV- Mock+SA：TLR3 slow virus carrier cocaine groups.

Figure 20 C57 mouse nucleus accumbens septi locating injection TLR3 inhibitor inhibits cocaine Conditioned place halo effect.p< 0.05, there is significant difference compared with the control group.DMSO+CO：Solvent control cocaine group；Inhibitor+CO：TLR3 inhibits Agent cocaine group；DMSO+CO：Solvent control physiological saline group；Inhibitor+CO：TLR3 inhibitor physiological saline groups.

Figure 21 C57 mouse nucleus accumbens septi locating injection TLR3 inhibitor weakens cocaine spontaneous activity (displacement distance).*p< 0.05, there is significant difference compared with the control group.(DMSO+CO：Solvent control cocaine group；Inhibitor+CO：Inhibitor can Cacaine group；DMSO+CO：Solvent control physiological saline group；Inhibitor+CO：Inhibitor physiological saline group)

Figure 22 C57 mouse nucleus accumbens septi locating injection TLR3 inhibitor weakens cocaine locomotor sensitivity effect (with baseline ratio Value).*p<0.05, there is significant difference compared with the control group.DMSO+CO：Solvent control cocaine group；Inhibitor+CO：Suppression Preparation cocaine group；DMSO+CO：Solvent control physiological saline group；Inhibitor+CO：Inhibitor physiological saline group.

IKK β up-regulated expressions in nucleus accumbens septi after Figure 23 C57 mouse cocaine multiple dosings.A:Western blot results； B:IOD analyzes statistical chart.*p<0.05, there is significant difference compared with the control group.Cocaine：Cocaine group；Saline：Physiology Brine group.

PIkB alpha expressions raise in nucleus accumbens septi after Figure 24 C57 mouse cocaine multiple dosings.A:Western blot results； B:IOD analyzes statistical chart.*p<0.05, there is significant difference compared with the control group.Cocaine：Cocaine group；Saline：Physiology Brine group.

Nucleus accumbens septi endochylema NF- κ B are expressed after Figure 25 C57 mouse cocaine multiple dosings.A:Western blot results；B: IOD analyzes statistical chart.*p<0.05, there is significant difference compared with the control group.Cocaine：Cocaine group；Saline：Physiology salt Water group.

NF- κ B are expressed in nucleus accumbens septi after Figure 26 C57 mouse cocaine multiple dosings.A:Western blot results；B: IOD analyzes statistical chart.*p<0.05, there is significant difference compared with the control group.Cocaine：Cocaine group；Saline：Physiology salt Water group.

Influence of Figure 27 C57 mouse brains locating injection TLR3 inhibitor to cocaine mouse nucleus accumbens septi pIKB alpha expressions.*p< 0.05, there is significant difference compared with the control group.Inhibitor+Cocaine：Inhibitor brain locating injection cocaine group； Inhibitor+Saline：Inhibitor brain locating injection physiological saline group；DMSO+Cocaine：Solvent control brain locating injection can Cacaine group；DMSO+Saline：Solvent control brain locating injection physiological saline group.

The shadow that Figure 28 C57 mouse brains locating injection TLR3 inhibitor expresses NF- κ B in cocaine mouse nucleus accumbens septi core It rings.*p<0.05, there is significant difference compared with the control group.Inhibitor+Cocaine：Inhibitor brain locating injection cocaine Group；Inhibitor+Saline：Inhibitor brain locating injection physiological saline group；DMSO+Cocaine：Solvent control brain positioning note Penetrate cocaine group；DMSO+Saline：Solvent control brain locating injection physiological saline group.

Figure 29 TLR3 mouse brain locating injections TLR3 expresses slow virus carrier to pIKB α in cocaine mouse nucleus accumbens septi core The influence of expression.*p<0.05, there is significant difference compared with the control group.LV-TLR3+Cocaine：TLR3 expresses slow virus brain Locating injection cocaine group；LV-TLR3+Saline：TLR3 expresses slow virus brain locating injection physiological saline group；LV-Mock+ Cocaine：Slow virus compares brain locating injection cocaine group；LV-Mock+Saline：Slow virus compares brain locating injection physiology Brine group.

Figure 30 TLR3 mouse brain locating injections TLR3 expresses slow virus carrier to NF- κ B in cocaine mouse nucleus accumbens septi core The influence of expression.*p<0.05, there is significant difference compared with the control group.LV-TLR3+Cocaine：TLR3 expresses slow virus brain Locating injection cocaine group；LV-TLR3+Saline：TLR3 expresses slow virus brain locating injection physiological saline group；LV-Mock+ Cocaine：Slow virus compares brain locating injection cocaine group；LV-Mock+Saline：Slow virus compares brain locating injection physiology Brine group.

Specific implementation mode

1 TLR3 inhibitor for treating cocaine habituations of experimental example

1 foreword

In our current research, we utilize gene Knockout, slow virus carrier from science of heredity and pharmacology angularly Structure and micromolecular inhibitor etc. carry out multi-angle confirmation, find that TLR3 participates in adjusting the mistake of mouse cocaine habituation for the first time Journey.During the experiment, joint CPP models, self administration model and spontaneous activity model, prove indirectly from another angle clear TLR3 inhibitor can more To treat cocaine habituation.

2 experiment materials and method

2.1 drug

Cocainehydrochloride：Identify that institute, white powder, purity are more than 99% purchased from Chinese pharmaceutical biological product, product batch number： 171210-200803。

Physiological saline：With Kelun Pharm Ind Co., Ltd., Sichuan, authentication code：Chinese medicines quasi-word H51021158, production batch Number：M12101307.

Cocainehydrochloride normal saline dilution to required concentration.

The TLR3 inhibitor that this experiment uses is compound (R) -2- (3-Chloro-6-fluorobenzo [b] Thiophene-2-carboxamido) -3-phenylpropanoic acid, structural formula：

Purchased from Merck Millipore Corp. (614310 | TLR3/dsRNA Complex Inhibitor-Calbiochem, catalogue number 614310-10MGCN).

2.2 experimental animal

Male and healthy sexal maturity (10~12 weeks) C57BL/6J mouse, 20~22g of weight are tested purchased from Shanghai City Si Laike Animal Co., Ltd provides (production licence number：SCXK (Shanghai) 2007-0005), 20~22g of weight.

TLR3 knock out mice strains are B6N.129S1-Tlr3tm1Flv/J, number：009675；MyD88 clpp genes Except mouse species are B6.129P2 (SJL)-Myd88tm1Defr/J, number：008888.Both the above Gene Knock-Out Animal Model is purchased from The U.S. laboratories Jackson are presented by biological therapy National Key Laboratory of Sichuan University.Two kinds of animals all have fertility energy Power.

All animal feeding conditions：Center SPF grades of animal house of national Chengdu new drug safety evaluatio, 20~25 DEG C of temperature, Relative humidity 55~65%, feeding environment meet national standard GB14925-2001, experimental animal licensing：SCXK (river) 2003-01.

All zoopery operations of this project meet International Laboratory Animal feeding management assessment and approve association (AAALAC) it requires.In whole experiment process, animal freely ingests and drinks water, and normally breeds experimental animals certain time before experiment To be familiar with and adapt to environment.

2.3 key instrument

Electronic analytical balance (Shanghai balance equipment factory)

Clean bench (Medical Equipment Plant of Shanghai Boxun Industrial Co., Ltd.)

DK-8D type electric heating constant temperatures sink (Shanghai gloomy reliable test Instrument Ltd.)

High speed low temperature centrifugal machine (German eppendorf company MR1812)

Electric heating constant temperature sink (Shanghai gloomy reliable test Instrument Ltd.)

Mouse stainless steel metabolism cage tool (Shanghai is same to educate medical anatomy model manufacturing company)

Rats and mice Place Preference case (Huaibei Zhenghua Biological Instrument Co., Ltd.)

Self administration system (An Lai Instrument Ltd.)

Electrophoresis tank (Bio-Rad companies of the U.S.)

Liquid-transfering gun (German eppendorf companies)

Liquid nitrogen container (Chengdu liquid nitrogen container factory)

2.4 experiment reagent

100% ethyl alcohol (Solution on Chemical Reagents in Shanghai Co., Ltd)

75% ethyl alcohol (is prepared) with the water that DEPC is handled

0.01M EDTA (Huamei Bio-Engrg Co.)

Formaldehyde (Solution on Chemical Reagents in Shanghai Co., Ltd)

Formaldehyde (Ambion)

Gold View dyestuffs (match Bai Sheng gene technology Co., Ltd in Shanghai)

Anti- beta-actin antibody (CST companies of the U.S.)

Anti- NF- kappa B antibodies (CST companies of the U.S.)

Anti- pIKB Alpha antibodies (CST companies of the U.S.)

Anti- IKK β antibody (CST companies of the U.S.)

Remaining reagent is that domestic analysis is pure.

2.5TLR3 expresses the structure of slow virus carrier

2.5.1 implementation sequence

1) carrier information：

Carrier is pReceiver-Lv201, and Fig. 1 is carrier figure.Target gene fragment TLR3 primers, upstream：5’-atg aacggt gtt cctctt atc taa tgt act cct ttg c-3’；Downstream：5’-ggg gac ttg tcc cta tgg att ctt ctg gtg tct ctag-3’。

2) competent cell and conversion are prepared

CaCl₂Method prepares competent cell progress transformation experiment, and steps are as follows：(1) each competent cell suspension is respectively taken 200 μ L are transferred in sterile eppendorf tubes, and often connection 10 μ L of liquid are added in pipe, gently rotate mixing, then set in ice and place 30min.Competent cell is prepared, the ability of intake exogenous DNA is made it have.(2) 42 DEG C of heat shock 90s, quickly by centrifuge tube It is transferred to cooling cell 1-2min in ice bath.Often 800 μ L LB culture mediums are added in pipe, and water-bath is heated up to 37 DEG C, then places shaking table 45min is incubated with recovery bacterium.(3) the LB agar that 150 μ L transformed competence colibacillus cells are transferred to AMP (100 μ g/mL) resistance is trained It supports on base.Tablet is placed in room temperature until liquid is absorbed.Then it is inverted plate, is placed in 37 DEG C of incubators and cultivates 16h.(4) Clone carries out follow-up PCR identifications.

2.5.2 construction of recombinant plasmid

PCR product is connected is shown in Table 1 into linearisation expression vector reaction system, reaction condition:25℃ 30min；42℃ 15min。

1 PCR reaction systems of table

X：The volume number of linearized vector DNA；Y：PCR product fragmental volumes number after purification.

2.5.3 virus packaging

293T cells are digested, it is to have 1.2X10 per 20mL to adjust its density⁷A cell, inoculating cell are put in culture dish 37 DEG C are set, 5%CO₂It is cultivated in incubator for 24 hours, can be used for cell transfecting when cell density reaches 70%-80%.Cell state pair It is most important in virus packaging, it needs to ensure good cell state and less passage number.2h will contain tire ox blood before transfection Clear culture medium is changed to serum free medium.Be added into centrifuge tube preparation each DNA solution (20 μ g of pGC-LV carriers, 15 μ g of pHelper1.0 carriers, 2.0 pHelper carrier, 10 μ g) it is uniformly mixed with respective volume culture medium, adjustment total volume is 2.5mL incubates 5min at room temperature.Lipofectamine2000 reagents are softly shaken up, 100 μ L are taken Lipofectamine2000 reagents are mixed in another Guan Zhongyu 2.4mL Opti-MEM culture mediums, incubate 5min at room temperature.Dilute DNA after releasing is mixed with the Lipofectamine2000 after dilution, and mixing of gently running avoids vibrating, and must be mixed in 5min It closes.20min is incubated after mixing at room temperature, then mixed liquor is transferred in 293T cell culture fluids, is uniformly mixed, places 37 DEG C, 5%CO₂It is cultivated in incubator.Go culture medium, every bottle of cell that 20mL phosphate buffers (PBS) are added after culture 8h, with The remaining mixed liquor of washing, removes mixed liquor.It is added in every bottle of cell and contains 10% fetal calf serum culture medium 25mL, 37 DEG C of placement, 5% CO₂Continue culture 2 days in incubator.

2.5.4 titer determination

1) sample preparation

293T cells pass on, and are added 1 × 10 in 24 holes per hole⁵A cell, volume are 500 μ L；Next day preparation 10 is sterile Ep is managed, per 90 μ L culture mediums of Guan Zhongjia；It takes 10 μ L of virus stock solution used to be measured to be added in first pipe, is uniformly mixed, take mixing equal Even 10 μ L of the first pipe liquid, which are added in second pipe, continues an identical operation to the last pipe；Cell hole needed for choosing is inhaled Remove 90 μ L culture mediums.Add the viral solution diluted, places 37 DEG C, 5%CO₂Incubator culture；After 1 day, fresh cultured is added 500 μ L of base.Careful operation, extracting RNA after 4 days.

2) total serum IgE extracts

Cell supernatant is gone, 1mL Trizol are added per hole, piping and druming is stored at room temperature 5min, is transferred to another new 1.5mL In Ep pipes.Often pipe plus 200 μ L chloroforms, firmly shake 15s, are stored at room temperature 15min.120000r/min centrifuges 15min at 4 DEG C.From Often in pipe in Aspirate supernatant to another new 1.5mL Ep pipes.The isopropanol of isometric -20 DEG C of precoolings is added, -20 DEG C after mixing Precipitate 10min.120000r/min centrifuges 10min at 4 DEG C, removes supernatant.75% ethyl alcohol of 1mL4 DEG C of precooling, washing is added Precipitation and centrifugation tube wall.10000r/min centrifuges 5min at 4 DEG C, and supernatant is abandoned in shifting.10000r/min is centrifuged again at 4 DEG C 5min sucks raffinate, dries at room temperature, is not required to be completely dried.Add 20 μ L without RNA enzyme (RNase) water to being completely dissolved, ultraviolet point Analysis measures institute's extracting RNA concentration.

3) RNA reverse transcriptions obtain cDNA

PCR tubules, coke booster diethyl carbonate (DEPC) is added in 1 μ L Oligo dT (0.5 μ g/ μ L) and 2.0 μ g total serum IgEs Water is to 9 μ L.It is uniformly mixed, centrifugation, 70 DEG C of warm bath 10min.And then it is inserted into 0 DEG C of ice-water bath.According to the form below ratio, according to anti- Should pipe figure out required amount of reagent.M-MLV enzymes etc. are uniformly mixed so as to obtain reverse transcription reaction liquid on ice.Add 11 in each reaction tube μ L reverse transcription reaction liquid, centrifuges after mixing.Wherein, 11 μ L reverse transcription reaction liquid containing 5 × RT Buffer, 4 μ L, 10mmol/LdNTPs2 μ L, 0.5 μ L of RNA inhibitor, M-MLV-RTase1 μ L, 3.5 μ L of DEPC water.It is inverse that 1h completions are carried out at 42 DEG C Responsive transcription, it is rear so that reverse transcriptase is inactivated with 70 DEG C of processing 10min.Reverse transcription reaction product cDNA can be used for PCR, also can -80 DEG C long-term preservation.

4) real-time quantitative PCR detects

Configure reaction system：Often pipe be added SYBRpremix ex Taq10 μ L, sense primer (5 μm of ol/L) 1.0 μ L, under Swim primer (5 μm of ol/L) 1.0 μ L, cDNA1.0 μ L, ddH₂O 7.5μL.Setting program is two-step method real-time quantitative PCR.Pre-degeneration 95℃ 5s；95 DEG C of 5s are denaturalized, anneal 60 DEG C of 30s, extends 60 DEG C of 30s, 40 cycles.Extending stage reading extinction every time Value, for making melting curve.After PCR, 1min is denaturalized at 95 DEG C.55 DEG C are subsequently cooled to, keeps DNA double chain fully poly- It closes.To 95 DEG C since 55 DEG C, each step increases by 0.5 DEG C, keeps 30s, while reading light absorption value.By set point after two cycles Increase 0.5 DEG C.

2.5.5 the harvest of slow virus and concentration

Collect the 293T cell supernatants of 2d after transfecting, 4 DEG C, 4000 × g centrifugation 10min, to remove cell fragment.With In 0.45 μm of filter filtering supernatant to 40mL ultracentrifugation pipes.Viral crude extract sample is added in filter cup.It will filtering Cup is inserted into filtered solution collecting pipe, then 4000 × g is centrifuged to required viral concentration volume, time 15min.It is taken out after centrifugation Centrifugal device separates filter cup and filtered solution collection cups.Filter cup is tipped upside down on sample collection cup, centrifugal force is no more than 1000 × g, time 2min.Excessive speeds can make sample loss.Filter cup is removed from sample collection cup.In sample collection cup As viral concentration liquid.

2.6 nucleus accumbens septi brain area pipe layings

Brain area positioning pipe laying is the direct approach and common technology of brain area site-specific delivery of drugs.10% hydration chlorine is used in this research Postanesthetic mouse is fixed on stereotaxic apparatus by aldehyde (0.4g/kg weight) anesthetized mice, overhead shaving, after fixed Head is adjusted to horizontality, medicinal alcohol sterilizes skin, and surgical exposure skull removes skull surface with cotton balls and eye scissors Meninx, find bregma position, marking pen label；Stereotaxic instrument is adjusted, injection catheter (Shenzhen Rui Wode biotechnologies are disposed Co., Ltd, #62003, OD 0.48mm X ID 0.34mm), it returns to zero in bregma；Using bregma position as origin, reference location Coordinate (nucleus accumbens septi：AP+1.6；ML+1.5) injection needle is moved to the surface of injection site, injection needle is made just to have been connect with skull It touches, return to zero Y coordinate, slowly moves downward injection needle to 4.4mm depths, unclamps coordinate arm and remove, by dentistry powder and dilution tune To starchiness fixed sleeving lower part, it is connected with skull and achievees the purpose that fixed catheter.It is inserted into conduit cap (Shenzhen Rui Wode biologies section Skill Co., Ltd, #62102, OD 0.30mm), prevent catheter blockage, sewing-end top wound.After operation, mouse is protected Temperature is then placed in reviving in clean rearging cage, single single cage raising.Since hand second day after operation, daily rear leg muscle Benzylpenicillin sodium salt solution (160000U/ml, 0.1ml/ mouse) is injected, and loosens conduit cap every other day, it is ensured that clearness of catheter, mouse The convalescence after Miles operation be 7 days.Animal by this operation injects TLR3 inhibitor for studying nucleus accumbens septi.

2.7 nucleus accumbens septi locating injection slow virus

Postanesthetic mouse is fixed on brain and stood by 10% chloraldurate (0.4g/kg weight) anesthetized mice, overhead shaving On body position indicator, head is adjusted to horizontality, medicinal alcohol sterilizes skin, surgical exposure skull, with cotton balls and eye scissors The meninx for removing skull surface finds bregma position, marking pen label；Stereotaxic instrument is adjusted, according to the elements of a fix (AP+ 1.6；ML±1.5；DV-4.4), sick slowly to inject 0.5 μ l in 0.1 μ l/min rate bilateral nucleus accumbens septis using 33 type injection needles Poison after injection, slowly removes injection needle, sewing-end top skin.After operation, then heat preservation mouse is put to reviving Enter in clean rearging cage, single single cage raising.Since hand second day after operation, daily rear leg intramuscular injection Benzylpenicillin sodium salt solution The convalescence after Miles operation of (160000U/ml, 0.1ml/ mouse), continuous injection 3 days, mouse is 7 days.By the animal of this operation Inhibit PRMT1 expression slow virus regulation and control cocaine Conditioned place preference behaviouristics detections and spontaneous work for studying nucleus accumbens septi injection Dynamic behaviouristics detection.

2.8 mouse jugular veins intubation is detained art

The outer venous cannula of neck, which is detained art, is the basis of jugular vein self administration model foundation, and determines the model foundation One committed step of success or not.Operation consent, experimental animal adapt to 3-5 days at ambient conditions, and daily with experiment people Member's contact, stress reaction is generated to avoid experimental animal in operation and experimentation.Jugular vein intubation is hard using import silica gel Pipe (outer diameter 0.48mm, internal diameter 0.40mm, length about 5mm) and matched silica gel hose (length about 3mm) are prepared, hose For hard tube front end, when operation, is conducive to intubation.

When operation, chloraldurate (10%, 10ml/kg) anesthetized animal is injected intraperitoneally according to mouse weight, rejects back shoulder blade Osseous part and neck left collarbone position mouse hair, mouse head is towards experimenter and carries out supine posture and fixes, in left clavicle upper limb A longitudinal direction about 1cm notch is opened at position, and blunt separation subcutaneous tissue, vein, whole process can drip on a small quantity the left neck that dissociates outside Physiological saline prevents drying.Surgical thread ligatures vein distal end, and eye scissors cut diagonal cut joint on vein, with tweezers by before Vein is inserted at the cannula hose end of making, is knotted for surgical thread and is fixed intubation.A subcutaneous tunnel is subcutaneously made a call to from back channel neck Road makes the intubation other end be pierced by and fix from back to cervical incision, to be connect with drug delivery device line.A small amount of heparin sodium aqua (30U/ml) and antibiotic (160000U/ml, 0.1ml/ mouse) are injected in vivo through carrying on the back neck intubation, and physiological saline is used in combination to rush Washing prevents from remaining, it is ensured that after clearness of catheter, is sleeved on cannula distal end with duct cover, sutures back and neck wound.After operation, Mouse is kept the temperature to revival, then mouse is put into clean cage, single single cage raising.Since hand second day after operation, daily A small amount of heparin sodium and antibiotic are injected through intubation, ensures that intubation is unobstructed, prevents wound infection, the convalescence after Miles operation of mouse is 7 days. It is used for cocaine self administration habituation model by this postoperative animal.

2.9 model foundation

2.9.1 the foundation of cocaine condition position preference pattern

All operations meet AAALAC requirements in zoopery, and mouse Place Preference case is as shown in Figure 2：

1) start before testing 3-5 days, mouse is stroked by same operating personnel daily, animal is allowed to have one to operator Fixed adaptation.

2) mouse is put into CPP babinets, acclimatization training 3 days, number of the data that third day measures as pretest According to.Adaptation training and testing time are 15 minutes, when animal is when side residence time is more than 600 seconds, knock-out experiment group.

3) using animal residence time longer side as nature preference case, intraperitoneal injection is then carried out, gives medicament Amount is following (table 2).

4) administration number of times is alternating delivery three times.Administration group is put into nature preference case when giving physiological saline；Give hydrochloric acid When cocaine, it is put into non-natural preference case, every time training 15 minutes.Control group gives physiological saline and is put into corresponding babinet every time It is interior.

2 cocaine administration approach of table, dosage, administered volume summary sheet

5) CPP tests are carried out within second day after being administered, mouse is sequentially placed into intermediate transition case, allows its freedom in item It shuttles 15 minutes in part position preference case, and records each babinet residence time.

6) points for attention：Animal training should act light and slow；End after training should clean babinet, eliminate dynamic The influence of object smell.

7) time of non-preference case is subtracted by the time of preference case come statistical result, is analyzed using SPSS statistical softwares real It tests as a result, difference is indicated with mean ± standard deviation, compares cocaine administration group and saline control group with t check analysis methods Difference, p<0.05 is to have significant difference.

2.9.2 the foundation of cocaine self administration model

FRI programs are selected to be trained in this research, the Experiment Training time (Session Time) is 120 minutes, instruction Maximum injection number is 50 times, per injection cocaine 0.75mg/kg in white silk, and self administration system is as shown in Figure 3.Experiment is set It is set to:The invalid nose of experimental animal touching, which touches, not to be responded to, and is touched effective nose and is touched acquisition drug, carries out drug injection, while nose is tactile Lamp is lighted closes with room lamp, and the fan in self administration case is always on during the experiment.Modeling is divided into 3 stages, i.e., in advance Standby phase, surgery recovery phase, cocaine self administration phase.

1) probationary period

Before starting experiment, natural feeding experimental animal 3-5 days, experimenter strokes animal daily, reduces because that stress make With and interference caused by experimental result.

2) operation and convalescence

Animal receive jugular vein set pipe operation after, daily carry out heparin sodium washing pipe, ensure administration pipeline it is unobstructed.Restore 5-7 After it, chooses the good animal of recovery and carry out into a group experiment.

3) the cocaine self administration phase

Experimental animal is randomly divided into two groups, cocaine group (n=16) and physiological saline group (n=8).

Check whether drug in nose tentaculum, transfusion system and syringe is enough etc. before experiment.When experiment, animal is put Enter self administration case, neck conduit is connect with transfusion system, the door of babinet is closed, animal is allowed to be adapted in case 15-20 minutes Afterwards, then start training experiment.Experimental animal Behavior Monitor System automatically records and preserves experimental result, records effective nose tactile time Several, invalid nose touches number, cocaine injection number.

The successful standard of habituation model foundation:

1) formation operation formula conditioned reflex；

2) cocaine injection frequency stabilization；

3) the continuous three days laxative numbers of same group of animal, within 10% range of its average value.

2.9.3 the foundation of cocaine spontaneous activity model

Spontaneous activity in mice device is as shown in Figure 4：

1) start before testing 3-5 days, experimenter strokes mouse, and animal is allowed to adapt to operator.

2) mouse is put into spontaneous activity box, acclimatization training 3 days, adaptation training and testing time are 30 minutes.

3) after acclimatization training, animal is divided into cocaine group and control group, is administered before detection daily, administration time Number is 7 times.

4) points for attention：Animal training should act light and slow；End after training should clean babinet, eliminate dynamic The influence of object smell.

5) result analyzes experimental result using SPSS statistical softwares, and difference is indicated with mean ± standard deviation, p<0.05 is to have Significant difference.

2.10 nucleus accumbens septis are drawn materials and are prepared

Quick sacrificed by decapitation C57 mouse in 30 minutes, are then rapidly separated brain after Behavior test, with 4 DEG C of lifes After reason normal saline washing 3 times, is detached according to brain anatomical atlas and take out nucleus accumbens septi (Fig. 5.And it is put into liquid nitrogen flash freezer, it will after the completion of sampling Sample is stored in -80 DEG C of environment.

2.11 micro-injection process

Mouse carries out Naoliqing capsule, and head is picked hair and sterilized, and skin of head, coordinate setting injection part are cut off with scissors Position, syringe needle puncture cranium, and injection needle is pierced into mouse brain according to coordinate, then notes inhibitor or viral vectors It is mapped to nucleus accumbens septi.The injection of slow virus is with 0.1 l/ minutes speed injections of μ, 10 minutes, total l μ l.Micro-injection after injection Needle is placed in original position 3-5 minutes, so that injected material is fully spread at the tip of injection needle.

2.12 prepared by frozen section

Heart, by left ventricle quick needle insertion, right auricle of heart and the right side are exposed after 10% chloraldurate intraperitoneal anesthesia of animal rapidly Atrium portion cuts off notch, and successively the PBS with physiological saline and containing 4% paraformaldehyde is successively perfused rapidly through body circulation.It takes out small Mouse brain, after fixed (4 DEG C, 2-4h) after the PBS containing 4% paraformaldehyde, be successively soaked in 20% (4 DEG C, 20-24h) and It is dehydrated in the sucrose solution of 30% (4 DEG C, 24-48h), until mouse brain is sunk to the bottom.Dewatered brain is flat in forebrain with coronal-plane Face cuts 10 μ m-thick brain sections with freezing microtome, and -20 DEG C are stored in protection liquid (containing 30% sucrose and 30% ethylene glycol PBS)。

2.13 Western Blotting

2.13.1 nucleoprotein is extracted and is quantified with plasmosin

It is said according to Nuclear extract and suppressor proteins extraction agent box (green skies bio tech ltd, P0027) Bright book extraction nucleus accumbens septi nucleoprotein and slurry albumen.Key step includes：Dissection materials first few minutes, which are prepared, contains 1mM PMSF eggs The extraction agent A liquid of white enzyme inhibitor, fresh nucleus accumbens septi tissue is put into PBS (1mM PMSF) solution of 500ml, is used 200 μ l pipette tips blow and beat tissue repeatedly, and disrupting tissue to fine particle is then centrifuged for, and collect tissue precipitation, and albumen is then added and takes out Put forward reagent A and the mixed liquor (20 of B:1 ratio mixes, 1mM PMSF) 100 μ l, most high speed is acutely vortexed 5 seconds, and cell precipitation is complete It exposes the wealth and scatter entirely, ice bath 15min, most high speed is acutely vortexed 5 seconds, ice bath 1min, and most high speed is acutely vortexed 5 seconds, 4 DEG C 13, 000g centrifuges 5min, draws immediately after in supernatant to the plastic tube of a precooling, the suppressor proteins as extracted freeze (pay attention in -80 DEG C：When shifting supernatant, precipitation must not be touched, the upper of minimum volume can be retained in the top of precipitation Clearly, precipitation is touched on one side).

For precipitation, remaining supernatant (if the pollution of suppressor proteins can be brought by not exhausting supernatant) is exhausted completely, is added Enter the Nuclear extract extraction agent that 30 μ l are added to PMSF.Most high speed is acutely vortexed 30 seconds, and cell precipitation is suspended simultaneously completely It scatter, then puts back in ice bath, high speed is acutely vortexed 30 seconds each 2min again, total 30min；4 DEG C of 13,000g centrifugations 10min is drawn immediately in supernatant to the plastic tube of a precooling, and the Nuclear extract as extracted freezes in -80 DEG C.

2.13.1 total protein extraction and quantitative

(1) tissue frozen after dissection is taken, RAPA lysates are added, is placed in and cracks 5 minutes on ice.If it is tissue extraction RIPA is added according to tissue size in albumen, and the RIPA amounts that nucleus accumbens septi tissue is added are about 100 μ l or so.

(2) ultrasound 10 times, 2 seconds/time (set on ice), it is therefore an objective to clasmatosis.

(3) 13,000g, 15 minutes, after 4 DEG C of centrifugations, supernatant is taken out, is put on ice.

(4) protein quantification

A BSA) is diluted to 1,0.5,0.4,0.3,0.2,0.1,0.05,0mg/ml concentration gradient (table 3).

B the protein liquid of extraction) is diluted 10-20 times.BSA and protein sample inhale 10 μ l and other hole are added respectively.

C the Coomassie brilliant blue G250 of 200 μ l) will be added in the hole for adding BSA and protein sample.

D) 595nm measures absorbance, linear dependence R2>0.99, calculate corresponding albumen concentration.

E on the basis of) using Cmin, suitable RIPA is added in remaining sample, is diluted to same concentration.

F) 5 × loading buffer (mercaptoethanol containing beta-) are added in each sample, and it is the 1/5 of albumen volume to measure.

(5) it boils sample 5 minutes, dispenses, volume and concentration calculate final quantity as 30 μ g or so.

Points for attention：Whole operation carries out on ice.It is accurate when protein quantification, after Coomassie brilliant blue G250 is added, to the greatest extent Amount is protected from light.

BSA dilution ratios table when 3 protein quantification of table

2.13.2 preparative separation glue (table 2-2)

4 separation gel of table is prepared with tabulation

Pay attention to：10%APS and TEMED is finally rapidly joined successively, is mixed well but not generated bubble.Encapsulating immediately, Every piece of glue needs about 5ml or so；Then deionized water is added along glass plate and completely cuts off air.Setting time is 15-30 minutes.

2.13.3 concentration glue (table 5) is prepared

Table 5 concentrates glue and prepares with tabulation

Pay attention to：10%APS and TEMED is finally rapidly joined successively, is mixed well but not generated bubble.Concentration glue is added Enter in glass plate, plug comb, solidifies 15-30 minutes.

2.13.4 electrophoresis prepares

The glass plate for having solidified glue is put into electrophoresis tank, 30% acrylamide/methene acrylamide is added.

30% acrylamide/methene acrylamide is prepared：Acrylamide：29g；Methene acrylamide：1g

First 50ml distilled waters is used to dissolve, stirring knows that solution is transparent, adds distilled water to final volume 100ml, filtration sterilization, Investigate the solution PH<7.0,4 DEG C of preservations of brown bottle.

2.13.5 point sample

It is about 20 μ l, 1mm point sample amounts is about 30 μ l that the protein content that each hole is added, which is about 30 μ g, 0.75mm point samples,.

2.13.6 electrophoresis

Concentrate glue voltage 80V, albumen to concentration glue separation gel boundary at, voltage adds as 120V, until albumen run through (according to Albumen size determines), the time is about 60 minutes.

2.13.7 transferring film

By in transferring film liquid everywhere iron pan, glue is put into separation gel in transferring film liquid.Transferring film folder puts sponge and respectively puts filter up and down Paper one is opened, and the glue cut is put on filter paper after complete wetting.It is another to prepare a culture dish methanol is added, it would be desirable to pvdf membrane It is soaked into wherein, then covers on glue.Transferring film voltage is set as 100V, and the time is depending on albumen size.

2.13.8 closing

5% skim milk, 25ml/ block glue are prepared with TBST.It is put into plate, by pvdf membrane complete wetting in milk In, it is placed on shaking table and shakes 1 hour.

2.13.9 incubating primary antibody

By the dilution proportion of primary antibody as required, sealed with hybrid belt, 4 DEG C overnight or 37 DEG C, 1-2 hours.Use TBST Wash film three times, it is 5-10 minutes each.

2.13.10 incubating secondary antibody

With 1：5000 are diluted in antibody in skim milk, 37 DEG C of shaking tables 1-2 hours, secondary, each 5- that washes film with TBST 10 minutes, it is primary then film to be washed with TBS, 5-10 minutes.

2.13.11 preparing luminescent solution and tabletting

Pvdf membrane is placed in luminescent solution in darkroom and is reacted 1 minute.Pvdf membrane is taken out, extra shine is blotted with filter paper Pvdf membrane is put into magazine by liquid, and face-up and film contact by film, compressing exposure, (time length determines that band is bright Degree), it develops a film.

2.13.12 gray value acquires

Immunoblotting film 6.0 systems of Image-Pro Plus are read into gray value, with beta-actin band gray scales Value is standardized as internal reference to be compared.

2.14 data analysis

All data are all made of standard error analysis, and ANOVA is used for the statistical significance of prediction data.All analyses are all made of SPSS11.5 softwares, p<0.05 is statistically significant for data.

3 experimental results

3.1 cocaines induce C57 mouse Conditioned place preferences

C57 mouse carry out acclimatization training in 3 days and the training of 6 days Conditioned place preferences, 3 cocaine dose group (n =12) (20mg/kg, 10mg/kg and 3mg/kg) and saline control group (n=12) condition position halo effect such as Fig. 6 institutes Show.After the Conditioned place preference training of cocaine, mouse significantly increases (P in cocaine training babinet residence time< 0.05), this shows that animal has generated pharmacological dependence, forms the conditioned reflex that cocaine award can be obtained in a certain environment, Wherein 20mg/kg cocaine doses group CPP effects are most strong.

3.2 TLR3 knock out mice cocaine Conditioned place preferences weaken

TLR3 knock out mice and MyD88 knock out mice carry out cocaine CPP detections, and cocaine dose is respectively 20mg/kg, 10mg/kg and 3mg/kg, control group are wild-type mice (WT) (Fig. 7-Fig. 9).It can be seen that with control group phase Than the CPP of TLR3 knock out mice and MyD88 knock out mice in various dose cocaine condition position preference modeling Effect is variant, and wherein TLR3 knock out mice induces case residence time more than wild-type mice in cocaine preference Short, TLR3 knock out mice conditions position halo effect significantly reduces, and has statistical significance (p compared with wild-type mice< 0.05).Under 20mg/kg cocaine doses, halo effect reduction in TLR3 knock out mice conditions position becomes apparent.The above knot Fruit shows that TLR3 knock out mice significantly reduces the habituation effect of cocaine, has prompted TLR3 may be in cocaine habituation Certain effect is played in the formation of process.

In the experiment of 3 various doses, for MyD88 groups, the cocaine condition preferences location effect compared with wild type Decrease, but equal no difference of science of statistics between two groups in all experiments.Above the experimental results showed that, MyD88 missing pair The condition position halo effect caused by cocaine does not make a significant impact, and the drug reward effect that cocaine generates may be with The association of TLRs/MyD88 Dependents is little, therefore, experiment backward we ground for the non-dependent approach of TLR3/MyD88 Study carefully.

3.3 TLR3 knock out mice cocaine spontaneous activities weaken

We continue to use effect of the spontaneous activity experimental study TLR3 genes in cocaine habituation behavior.TLR3 genes Knock-out mice acclimatization training 3 days, daily 30 minutes.The activity of 3 ten minutes animals was as baseline before the test same day, and the 4th Before starting within a ten minutes mouse peritoneal injection give 3,10,20mg/kg cocaines, then continuous 10min, 20min after surveying injection Until 9 time points such as 90min, each time point interval 10min.

After test result (Figure 10-Figure 12) shows cocaine injection, wild-type mice behavioral activity amount is gradually increasing, It peaks within 20-30 minutes after administration, gradually decreases later.TLR3 knock out mice locomotor activation after giving cocaine Also increase, but be less than wild-type mice group in part-time point locomotor activation, and have significant difference (p<0.05).Wherein, Under 20mg/kg cocaine doses, the reduction of TLR3 knock out mice locomotor activations becomes apparent.The above result shows that TLR3 Knock out mice reduces cocaine locomotor sensitivity effect, has prompted work of the TLR3 genes in participating in cocaine habituation benefit With.

3.4 TLR3 knock out mice cocaine self administration behaviors weaken

Experiment starts first 3 days, and animal carries out self administration system environments adaptation, daily 30min, to reduce because environment causes Experimental error.It tests the 1st day, there is no difference between four indexs, show to act on initial stage habituation effect not yet in cocaine It is formed, it is the exploratory behavior of itself that the nose of animal, which touches behavior, with the propulsion of subsequent experimental, finds the effective nose of animal cocaine Tactile number is continuously increased (Figure 13), and the effective nose of physiological saline group touches number during the experiment that significant changes (figure does not occur 14.After training 3-4 days, compared with TLR3 knock out mice, the effective nose of wild-type mice cocaine touches number and dramatically increases (p< 0.05), invalid nose touches number and initial level indifference, illustrates that wild-type mice has discrimination under the action of cocaine The ability of effective nose tentaculum and invalid nose tentaculum, it is established that operant conditioned reflex, on the contrary, this exactly illustrates TLR3 gene knockouts Mouse is substantially reduced by touching effective nose tentaculum to obtain the behavior of drug reward.In the experiment of physiological saline group animal, Effective nose of TLR3 knock out mice and wild-type mice touches number and invalid nose touches number and do not have in entire experiment process Notable difference illustrates both distinguish effective nose tentaculum and invalid nose tentaculum (Figure 15).In addition, since experiment the 5th day, The effective nose of wild-type mice cocaine touches number (drug injection number) and is maintained at a more stable level, illustrate itself to Medicine is trained so that cocaine touches effective nose tentaculum to animal is reinforced and consolidated to obtain drug reward this behavior, so And physiological saline is then without playing above-mentioned effect.It is obtained by Figure 15, TLR3 knock out mice and wild-type mice are to cocaine Demand show the trend gradually increased, started to tend to be steady by the 5th day, plateau occur, (same group of animal connects Continue three days laxative numbers within 10% range of its average value), still, compared with wild-type mice, TLR3 gene knockouts are small The intake of mouse cocaine significantly reduces (p<0.05).The above experimental result illustrates that TLR3 gene knockouts cause mouse to cocaine Demand weaken.

Enhance cocaine CPP effects after 3.5 TLR3 knock out mice intracerebral injections TLR3 expression slow virus

TLR3 knock out mice intracerebral locating injections TLR3 expresses slow virus carrier, and injection point is bilateral nucleus accumbens septi.It is dynamic After object wound is restored, heart perfusion and fixed brain, frozen section, fluorescence microscopy microscopic observation virus injection position (Figure 16). Animal carries out acclimatization training in 3 days, daily 15-20min.It is detected by the Conditioned place preference of cocaine, with intracerebral injection The animal of viral vectors control is compared, and the mouse for having injected TLR3 slow virus carriers is aobvious in cocaine induction babinet residence time It writes and increases (P<0.05) after, this illustrates intracerebral injection TLR3 expression slow virus, the cocaine habituation effect of TLR3 knock-out mices is bright Aobvious enhancing, the result (Figure 17) show：After TLR3 knock-out mice nucleus accumbens septis TLR3 is expressed again, cocaine habituation can be restored Behavior effect.

3.6 TLR3 knock out mice intracerebral injections TLR3 expression slow virus increase cocaine spontaneous activity

TLR3 knock out mice intracerebral bilateral nucleus accumbens septis inject TLR3 and express slow virus carrier, after wounds in animals is restored, It is grouped and carries out environment adaptation training, each 30min, to reduce influence of the environment to experimental result at random.Formally start to test It surveys spontaneous activity baseline within first 3 days, tests the 4th day beginning abdominal cavity and give cocaine.As seen from Figure 18, work in 3 days before four groups of animals Momentum is in same level, and basic activity does not have notable difference, but since being administered the 4th day, the TLR3 expression of brain locating injection The locomotor activation of slow virus carrier animal has apparent increase, and has compared with the animal of injecting lentivirus vehicle Control Significant statistical significance (P<0.05).To keep experimental result more objective, eliminate because each group animal basis activity difference is right Influence caused by experimental result, daily locomotor activation makees ratio with own foundation activity before administration after every group of animal is administered Value, as shown in figure 19, the locomotor activation and injecting lentivirus carrier pair of brain locating injection TLR3 expression slow virus carrier animals According to animal compared to dramatically increasing, and equally there is statistical significance (P<0.05).Above the experimental results showed that：TLR3 clpp genes After TLR3 is expressed in nucleus accumbens septi region again in animal brain, the spontaneous activity of animal increases, locomotor sensitivity caused by cocaine It is more obvious.

3.7 C57 mouse TLR3 inhibitor intracerebral injection cocaine CPP effects weaken

C57 mouse are carried out TLR3 inhibitor brain locating injections after wounds in animals is restored to be grouped at random and carry out cocaine CPP is trained.For two groups of animals of cocaine, brain locating injection TLR3 inhibitor mouse conditions position halo effect is injected intraperitoneally It is apparent to weaken (P<0.05) (Figure 20)；However, the unconditional position preference effect of two groups of animals of intraperitoneal injection of saline.More than The experimental results showed that：After C57 mouse brain locating injection TLR3 inhibitor, animal induces the residence time of case significantly to subtract in cocaine Few, demand of the animal to cocaine and the apparent decrease of dependence prompt TLR3 genes to play a role in cocaine habituation is formed.

3.8 C57 mouse TLR3 inhibitor intracerebral injection cocaine spontaneous activities reduce

Nucleus accumbens septi injects TLR3 inhibitor in C57 mouse brains, carries out spontaneous activity detection.As seen from Figure 21, before 4 groups of animals 3 days activities are in same level, and Activities quantitative baseline does not have notable difference.Since the 4th day, nucleus accumbens septi locating injection The cocaine administration mouse activity of TLR3 inhibitor, which increases, is significantly lower than solvent control group, and two groups have significant difference (P< 0.05).The result explanation：After inhibiting TLR3, cocaine locomotor sensitivity effect can be weakened.To keep experimental result more objective, disappear Except because of each group animal basis activity difference experimental result caused by influence, daily spontaneous activity after every group of animal is administered Amount makees ratio with own foundation activity before administration, as shown in figure 22, the spontaneous activity of brain locating injection TLR3 inhibitor animals Incrementss are significantly lower than the animal of control group, and equally have statistical significance (P<0.05).

3.8 cocaine multiple dosings activate mouse IKB α/NF- κ B signal accesses

C57 mouse after cocaine CPP training, put to death, and takes out nucleus accumbens septi (NAc) brain area, extracts albumen by the neck that breaks, with anti- IKK β, pIKB α and NF- kappa B antibodies carry out Western blot detections.As a result, it has been found that after cocaine CPP training, IKK in endochylema (P is obviously raised in the expression of β, pIKB α and NF- κ B<0.05) (Figure 23 -- Figure 25).NF- κ B in nucleoprotein are detected, are found NF- κ B expression quantity equally raises (Figure 26) (P in core<0.05), illustrate that NF- κ B are detached with phosphorylation I κ B and entered core in endochylema Interior startup downstream signal transduction.The above result shows that cocaine CPP training makes inside and outside IKK β in mouse brain, pIkB α and nucleus NF- κ B expression quantity change, cocaine CPP training can activate NF-IkB accesses.Above the experimental results showed that TLR3 is participated in The process of cocaine habituation is adjusted, and prompts it possibly via TLR3/NF-kB approach to realize.

3.9 inhibit or knock out TLR3 decrease IKB α/NF- κ B signals transductions

The adjusting that mouse cocaine habituation process is participated in for confirmation TLR3, presses down C57 mouse nucleus accumbens septi locating injections TLR3 Preparation simultaneously carries out cocaine CPP detections.The disconnected neck of mouse is put to death, and is taken out nucleus accumbens septi, albumen is extracted, with anti-pIKB α and NF- kappa B antibodies Carry out Western blot detections.As a result, it has been found that after cocaine CPP training, in endochylema the expression of pIKB α obviously raise (Figure 27) (P<0.05), while in core NF- κ B expression quantity raises and has statistical significance (Figure 28) (P<0.05).The above result shows that suppression TLR3 can lower the expression quantity of key protein in IKB α/NF- κ B accesses in nucleus accumbens septi processed, that is, inhibit TLR3 that can weaken IKB α/NF- The signal transduction of κ B.

TLR3 knock out mice nucleus accumbens septi locating injections TLR3 expresses slow virus, and carries out cocaine CPP detections, takes out Nucleus accumbens septi sample carries out Western blot detections with the antibody of anti-pIKB α and NF- κ B.As a result, it has been found that cocaine CPP training Afterwards, (Figure 29) (P is obviously raised in the expression of pIKB α in the animal endochylema of brain locating injection TLR3 expression slow virus<0.05), together When, the equally up-regulation of NF- κ B expression quantity and there is statistical significance, (Figure 30) (P in core<0.05), the above result shows that TLR3 bases After TLR3 genes restore expression in knock-out mice nucleus accumbens septi, the table of NF- κ B in pIKB α and core has been raised in cocaine CPP training It reaches, illustrates that TLR3 genes restore cocaine CPP after expressing and have activated IKB α/NF- κ B accesses.

4 conclusions

This project mainly uses animal behavioral study and the means such as science of heredity and pharmacological intervention to probe into TLR3 and can block in mouse Because of the effect in habituation mechanism.

It was found that TLR3 knock out mice cocaine CPP effects significantly reduce, show that it subtracts the dependence of cocaine It is weak.Compared with wild-type mice, TLR3 knock out mice weakens the locomotor sensitivity effect that cocaine induces.In self administration In experiment, TLR3 knock out mice self administration numbers are considerably less than, and are shown to crave for cocaine and be weakened.

We construct TLR3 expression slow virus carriers, and in the TLR3 knock out mice nucleus accumbens septi locating injections virus Then carrier carries out related neural Behavior Test to restore TLR3 expression.Test result shows：TLR3 knock-out mice nucleus accumbens septis Cocaine addiction is significantly increased after injection TLR3 slow virus carriers.

We continue using TLR3 inhibitor locating injection in C57 mouse nucleus accumbens septis, then carry out Addictive Behaviors evaluation. As a result, it has been found that TLR3 inhibitor can weaken C57 mouse to the additive of cocaine.

Finally, the key protein of habituation mouse TLR3/IKK β/NF- κ B accesses is detected.As a result, it has been found that cocaine CPP training makes the equal up-regulated expression of NF- κ B expressions inside and outside IKK β in mouse brain, pIkB α and nucleus.But work as C57 mouse brains After locating injection TLR3 inhibitor, NF-kB lowers expression in endochylema pIKB α and core, in addition, TLR3 knock out mice passes through After brain locating injection slow virus carrier restores expression TLR3, NF- κ B up-regulated expressions in endochylema pIKB α and core.It these results suggest that TLR3 participates in cocaine habituation process, thereby increases and it is possible to be to participate in reconciling by TLR3/IKB α/NF- κ B accesses.

To sum up, TLR3 inhibitor can effectively weaken the dependence and craving to cocaine, weaken the behavior of cocaine induction Sensitizing effect can treat cocaine habituation.

Claims (4)

1. Purposes of the 1.TLR3 inhibitor in the drug for preparing treatment cocaine habituation；The TLR3 inhibitor is compound I, Structural formula is as follows：
2. 2. purposes according to claim 1, it is characterised in that：The drug of the treatment cocaine habituation is to reduce pIKB α With the drug of NF-kB expressions.
3. 3. purposes according to claim 1, it is characterised in that：The drug is added using TLR3 inhibitor as active constituent The preparation that upper pharmaceutically acceptable auxiliary material or complementary ingredient are prepared.
4. 4. purposes according to claim 3, it is characterised in that：The preparation is ejection preparation.