CN105236586A - Method for absorbing tetracycline antibiotic pollutants in water through ageratina adenophora - Google Patents
Method for absorbing tetracycline antibiotic pollutants in water through ageratina adenophora Download PDFInfo
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- CN105236586A CN105236586A CN201510777197.4A CN201510777197A CN105236586A CN 105236586 A CN105236586 A CN 105236586A CN 201510777197 A CN201510777197 A CN 201510777197A CN 105236586 A CN105236586 A CN 105236586A
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Abstract
The invention provides a method for absorbing tetracycline antibiotic pollutants in water through ageratina adenophora and belongs to the technical field of environment pollution treatment. Soil on roots of ageratina adenophora plants is removed, and the roots are fixed to floating objects and arranged in tap water for culture; when the fibrous root system of ageratina adenophora grows to 10-15 cm, for a static polluted water source, the floating objects on which ageratina adenophora is planted are transferred into the polluted water source for 5-10 days, and in each cubic meter of water, the plant density ranges from 40 to 100; for dynamic blow-off water, the floating objects on which ageratina adenophora is planted are transferred into a settling pond without a drain outlet, in each cubic meter of the water, the plant density ranges from 120 to 240, germinated new buds are removed every other 7-15 days, it is guaranteed that the plants have no apical dominance, and the floating objects on which ageratina adenophora is planted are replaced every other 45-50 days. Most of the tetracycline antibiotic pollutants in the water can be removed, and the treatment difficulty and cost of the tetracycline antibiotic pollutants are reduced.
Description
Technical field
The invention belongs to technical field of environment pollution control.
Background technology
Antibiotic use is even abused and is caused to physical environment the pollution that can not be ignored, and produces potential hazard to human body health.Tetracycline antibiotics is the class Broad spectrum antibiotics produced by streptomycete, comprise duomycin, terramycin, tsiklomitsin etc., because it can prevent and treat human and animal's disease caused by the bacteria, growth stimulant can be used as again to accelerate growth of animal simultaneously, at home and abroad be widely used at present.Tetracycline antibiotics residence time in water body, soil is longer, has become serious potential pollution source in recent years.Such microbiotic is by mode enrichments in natural water source fast such as medicine company factory effluent, hospital wastewater, agriculture production waste water, sanitary wastewaters, and the water pollution problem caused is particularly outstanding.The tetracycline medication how processed in water body pollutes the problem having become and be badly in need of solving.
Application number be 201310011876 Chinese patent document disclose " a kind of plant restoration method to tsiklomitsin pollutant effluents ", floating bed is made with porous plastics, punch and implant Herba pteridis vittatae, pending tsiklomitsin pollutant effluents is arranged in tank, floating bed is placed in tank and carries out water remediation.The method can only for the treatment of the waste water in tank, and application limitations is large.
Application number be 201210128457 Chinese patent document disclose a kind of Ageratina adenophora water culture method, the object of the method is not water pollution control, but be convenient to obtain consistent and high-quality Rooted Cuttings, for the research of the aspects such as the invasion mechanism of Hemp Eupatorium, chemistry and biological control, molecular biology and physiological ecological provides reliable material resources.
Although people have found that exotic plant Eupatorium adenophorum Spreng has good absorption and accumulation and turn-over capacity to the heavy metal in contaminated soil, can be used for the soil remediation in heavy metal contamination area, but do not absorb any report of tetracycline antibiotics pollutent in water body with Hemp Eupatorium so far.
Summary of the invention
The object of the invention is to utilize the tetracyclines pollutent in Hemp Eupatorium efficiently concentrating absorption sewage, a kind of method absorbing tetracycline antibiotics pollutent in water body with Hemp Eupatorium is provided, reduce intractability and the cost of tetracyclines pollution.
The inventive method is according to the following steps:
1, choose the plant that plant height is Hemp Eupatorium 20 ~ 50 centimetres, its root is removed soil, is fixed on floating matter, be placed in tap water and cultivate.
2, when Hemp Eupatorium fibrous root system grows to 10 ~ 15 centimetres:
For the polluted source of static state, be transferred in polluted source by planting the floating matter being implanted with Hemp Eupatorium, plant spacing is 40 ~ 100 strains/cubic meter water body; To be transferred on polluted source 5 ~ 10 days, i.e. the tetracycline antibiotics pollutent of the overwhelming majority in Absorbable rod water;
For dynamic sewer, be transferred in the settling tank without sewage draining exit by planting the floating matter being implanted with Hemp Eupatorium, plant spacing is 120 ~ 240 strains/cubic meter water body, the sprouting sent is beaten at interval of 7 ~ 15 days, ensure that plant is without apical dominance, changed the floating matter of once planting and being implanted with Hemp Eupatorium at interval of 45 ~ 50 days, most of tetracycline antibiotics pollutent in water body can be removed.
3, used Hemp Eupatorium is focused on, should prevent Hemp Eupatorium from blossoming and bearing fruit during use, escape or cause secondary pollution.
The invention has the beneficial effects as follows the method providing first and absorb tetracycline antibiotics pollutent in water body with Hemp Eupatorium, reduce intractability and the cost of tetracyclines pollution.
Embodiment
Embodiment 1: the Hemp Eupatorium plant choosing plant height about 20 ~ 50 centimetres, its root is removed soil, be fixed on porous plastics, be placed in tap water to cultivate, when Hemp Eupatorium fibrous root system grows to 10 ~ 15 centimetres, transfer them to tsiklomitsin (Biotopped) concentration and be respectively 0.02mg/ml, the water planting liquid of 0.05mg/ml and 0.10mg/ml (uses the preparation of 1g/L macroelement water-soluble fertilizer, ShenFang,SiChuan dolantin Industrial Co., Ltd. combines manufacture with PLANTA fertilizer company of the U.S.) and blank without in the nutrient solution of tsiklomitsin, the strain number of Hemp Eupatorium is 70 strains/m
3.After Hemp Eupatorium grows 10 days in water planting liquid, utilize supper-fast liquid chromatograph to analyse tsiklomitsin content that (PromineneceUFLC, Shimadzu, LC-20AD) measures enrichment in the stem of Hemp Eupatorium and leaf, result is as table 1.
the stem of table 1 Hemp Eupatorium and the situation of leaf enrichment tsiklomitsin
Embodiment 2: the Hemp Eupatorium plant choosing plant height about 20 ~ 50 centimetres, its root is removed soil, be fixed on porous plastics, be placed in tap water to cultivate, when Hemp Eupatorium fibrous root system grows to 10 ~ 15 centimetres, transfer them in the nutrient solution and blank nutrient solution that chlortetracycline concentration is respectively 0.02mg/ml, 0.05mg/ml and 0.10mg/ml, the strain number of Hemp Eupatorium is 50 strains/m
3.After Hemp Eupatorium grows 12 days in water liquid, utilize supper-fast liquid chromatograph to analyse duomycin content that (PromineneceUFLC, Shimadzu, LC-20AD) measures enrichment in the stem of Hemp Eupatorium and leaf, result is as table 2.
the stem of table 2 Hemp Eupatorium and the situation of leaf Concentration of Gold mycin
Embodiment 3: the Hemp Eupatorium plant choosing plant height about 20 ~ 50 centimetres, its root is removed soil, be fixed on porous plastics, be placed in tap water to cultivate, when Hemp Eupatorium fibrous root system grows to 10 ~ 15 centimetres, transfer them in 1 cubic metre of detention tank of simulation, plant spacing is 100 strains/cubic meter water body; In settling tank water, tsiklomitsin (Biotopped) concentration is 0.05 ~ 0.1mg/ml; Plant and remove after 10 days, utilize supper-fast liquid chromatograph to analyse in (PromineneceUFLC, Shimadzu, LC-20AD) dynamic measurement water body tsiklomitsin data as table 3.
Table 3 different concns tsiklomitsin static cultivation tetracycline concentration in nutrient solution after 10 days
Example 4: the Hemp Eupatorium plant choosing plant height about 20 ~ 50 centimetres, its root is removed soil, be fixed on porous plastics, be placed in tap water to cultivate, when Hemp Eupatorium fibrous root system grows to 10 ~ 15 centimetres, shifted and be fixed in flowing water system, water body flow speed is not more than 1m/s, and plant spacing is 200 strains/cubic meter water body; The sprouting newly sent is beaten at interval of 12 days, ensure that plant is without apical dominance, plant and remove after 48 days, supper-fast liquid chromatograph is utilized to analyse (PromineneceUFLC, Shimadzu, LC-20AD) dynamic measurement plant different sites tetracycline medication total content data are as table 4, table 5, table 6.
The dynamic content of tsiklomitsin in the old blade of table 4
The dynamic content of tsiklomitsin in table 5 stem
The dynamic content of tsiklomitsin in table 6 young leaves and bud
Claims (1)
1. absorb a method for tetracycline antibiotics pollutent in water body with Hemp Eupatorium, it is characterized in that according to the following steps:
(1), choose the plant that plant height is Hemp Eupatorium 20 ~ 50 centimetres, its root is removed soil, is fixed on floating matter, be placed in tap water and cultivate;
(2), when Hemp Eupatorium fibrous root system grows to 10 ~ 15 centimetres:
For the polluted source of static state, be transferred in polluted source by planting the floating matter being implanted with Hemp Eupatorium, plant spacing is 40 ~ 100 strains/cubic meter water body, to be transferred on polluted source 5 ~ 10 days;
For dynamic sewer, be transferred in the settling tank without sewage draining exit by planting the floating matter being implanted with Hemp Eupatorium, plant spacing is 120 ~ 240 strains/cubic meter water body, the sprouting sent is beaten at interval of 7 ~ 15 days, ensure that plant is without apical dominance, changes the floating matter of once planting and being implanted with Hemp Eupatorium at interval of 45 ~ 50 days;
(3), by used Hemp Eupatorium focus on, should prevent Hemp Eupatorium from blossoming and bearing fruit during use, escape or cause secondary pollution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510777197.4A CN105236586B (en) | 2015-11-14 | 2015-11-14 | Method for absorbing tetracycline antibiotic pollutants in water through ageratina adenophora |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510777197.4A CN105236586B (en) | 2015-11-14 | 2015-11-14 | Method for absorbing tetracycline antibiotic pollutants in water through ageratina adenophora |
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| CN105236586A true CN105236586A (en) | 2016-01-13 |
| CN105236586B CN105236586B (en) | 2017-05-03 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS57145816A (en) * | 1981-03-06 | 1982-09-09 | Wakunaga Yakuhin Kk | Assistant for absorption of drug |
| CN101602527A (en) * | 2009-07-14 | 2009-12-16 | 杨云 | High-efficiency plant sewage-treating agent and with its method of disposing of sewage |
| US20100151029A1 (en) * | 2004-02-27 | 2010-06-17 | Hydromer, Inc. | Anti-infectious hydrogel compositions |
| CN103043801A (en) * | 2013-01-14 | 2013-04-17 | 山东大学 | Plant restoration method of tetracycline-polluted wastewater |
| CN103848502A (en) * | 2012-12-05 | 2014-06-11 | 中国科学院城市环境研究所 | Aquatic plant filter system for purifying breeding wastewater containing antibiotics |
-
2015
- 2015-11-14 CN CN201510777197.4A patent/CN105236586B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS57145816A (en) * | 1981-03-06 | 1982-09-09 | Wakunaga Yakuhin Kk | Assistant for absorption of drug |
| US20100151029A1 (en) * | 2004-02-27 | 2010-06-17 | Hydromer, Inc. | Anti-infectious hydrogel compositions |
| CN101602527A (en) * | 2009-07-14 | 2009-12-16 | 杨云 | High-efficiency plant sewage-treating agent and with its method of disposing of sewage |
| CN103848502A (en) * | 2012-12-05 | 2014-06-11 | 中国科学院城市环境研究所 | Aquatic plant filter system for purifying breeding wastewater containing antibiotics |
| CN103043801A (en) * | 2013-01-14 | 2013-04-17 | 山东大学 | Plant restoration method of tetracycline-polluted wastewater |
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| CN105236586B (en) | 2017-05-03 |
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Granted publication date: 20170503 Termination date: 20171114 |