CN105232407A - Bromelain whitening lotion and preparation method thereof - Google Patents

Abstract

The invention provides bromelain whitening lotion and a preparation method thereof. The lotion is prepared from the following components in parts by weight: 0.5-1 part of bromelain, 2-10 parts of hyaluronic acid, 50-70 parts of humectant, 2-20 parts of carbomer, 10-20 parts of polyoxyethylene sorbitan monolaurate, 2-20 parts of n-hexyl glucoside, 5-20 parts of triethanolamine, 2-10 parts of oleic alcohol polyoxyethylene ether, 1-2 parts of preservative, 10-20 parts of herbal extract, 5-20 parts of antioxidant and 840-880 parts of deionized water. The preparation method comprises the steps of preparing bromelain, preparing a base solution, preparing an emulsion and compounding. The lotion is prepared by using natural substances as whitening active ingredients, has the advantages of light and thin texture and good absorption, and cannot easily cause skin allergy.

Description

A kind of bromelain tender white skin softening water and preparation method thereof

technical field

The invention relates to the technical field of skin care products, in particular to a bromelain whitening and softening lotion and a preparation method thereof.

Background technique

Bromelain (Bromelain, referred to as bromelain, also known as bromelain or bromelain) is a pure natural product extracted from pineapple stems, leaves, and skins, refined, purified, concentrated, enzyme immobilized, and freeze-dried. plant protease. Its appearance is light gray powder, its molecular weight is 33000, and its isoelectric point is 9.55. Bromelain is soluble in water but insoluble in ethanol, chloroform and ether. Bromelain has excellent effects of skin rejuvenation, whitening and speckle removal. Basic principle of action: bromelain can act on the aging stratum corneum of human skin to promote its degradation, decomposition and removal, promote skin metabolism, reduce skin color darkening caused by sun exposure, and make the skin maintain a good white and tender state.

Contents of the invention

The object of the present invention is to provide a bromelain whitening and softening water with skin rejuvenation and whitening effect.

In order to achieve the above object, the technical scheme adopted in the present invention is as follows:

A bromelain whitening and softening lotion is made of the following components by weight: 0.5-1 weight part bromelain, 2-10 weight parts hyaluronic acid, 50-70 weight parts moisturizing agent, 2-20 parts by weight of carbomer, 10-20 parts by weight of polyoxyethylene sorbitan monolaurate, 2-20 parts by weight of n-hexyl glucoside, 5-20 parts by weight of triethanolamine, 2- 10 parts by weight of polyoxyethylene oleyl ether, 1-2 parts by weight of preservatives, 10-20 parts by weight of plant extracts, 5-20 parts by weight of antioxidants and 840-880 parts by weight of deionized water;

Described humectant is any one or its mixture in propylene glycol, butylene glycol, glycerin, polyethylene glycol; Described preservative is methylparaben and/or propylparaben; Described anti- The oxidant is one of tea polyphenols, rosemanol, bamboo leaf flavonoids and lycopene;

The plant extract is any one of rose extract, kiwi fruit extract, lemon extract and papaya extract.

As preferably, described bromelain is made by the following steps:

a) Juicing: Wash the pineapple leftovers, squeeze out the juice with a press, and filter to get the filtrate;

b) Adsorption: adding anhydrous calcium chloride to the filtrate obtained in step a), stirring and adsorbing, removing the supernatant to obtain the adsorbate;

c) Elution: adjust the pH value of the adsorbate to 7.0 with 1M dilute hydrochloric acid, add EDTA for elution, centrifuge, and collect the eluate;

d) Salting out: adjust the pH value of the eluent to 5.0 with 1M dilute hydrochloric acid, add ammonium sulfate powder, wait for complete dissolution, salt out at 4°C, and centrifuge to collect the precipitated salted out product, that is, the crude enzyme;

f) Refining: Dissolve the crude enzyme, adjust the pH to 7.0-7.5 with phosphate buffer, filter and stir to collect the filtrate, adjust the pH value to 4.0 with 1M dilute hydrochloric acid, let the enzyme precipitate out, centrifuge to take the precipitate, -80 Freeze-dry at ℃ to get bromelain.

Another object of the present invention is to provide the preparation method of the above-mentioned bromelain whitening and softening water, comprising the following steps:

1) prepare bromelain: prepare bromelain with pineapple leftovers;

2) Prepare the base solution:

Add 2-10 parts by weight of hyaluronic acid into 200 parts by weight of deionized water, heat to 90-95°C, keep the temperature for 20-30 minutes, and keep stirring until the hyaluronic acid is completely dissolved; then add 2-20 parts by weight 1 part of carbomer, 200 parts by weight of deionized water, heated and stirred, and kept at a temperature of 90-95°C for 60-90min, until the carbomer was completely dissolved to obtain a base solution;

3) Preparation of emulsion: add 50-70 parts by weight of humectant, 10-20 parts by weight of polyoxyethylene sorbitan monolaurate, and 5-20 parts by weight of triethanolamine to the base solution in step 2) , 2-20 parts by weight of n-hexyl glucoside, 2-10 parts by weight of polyoxyethylene oleyl ether, 1-2 parts by weight of preservatives and the rest of water, heating and stirring until all ingredients are completely dissolved and emulsified evenly, Cool to 70-80°C to obtain emulsion;

4) Compounding: Add 10-20 parts by weight of plant extracts and 5-20 parts by weight of antioxidants to the emulsion prepared in step 3), stir evenly, let stand, and wait until the temperature drops to 50°C, add 0.5 - 1 part by weight of bromelain, stirred to dissolve it, and after the bromelain is completely dissolved, let it stand for 24 hours to obtain the bromelain whitening and softening lotion.

As preferably, in step 1), preparing bromelain with pineapple leftovers comprises the following steps:

a) Juicing: Wash the pineapple leftovers, squeeze out the juice with a press, and filter to get the filtrate;

b) Adsorption: adding anhydrous calcium chloride to the filtrate obtained in step 1), stirring and adsorbing, removing the supernatant to obtain an adsorbate;

c) Elution: adjust the pH value of the adsorbate to 7.0 with 1M dilute hydrochloric acid, add EDTA for elution, centrifuge, and collect the eluate;

d) Salting out: adjust the pH value of the eluent to 5.0 with 1M dilute hydrochloric acid, add ammonium sulfate powder, wait for complete dissolution, salt out at 4°C, and centrifuge to collect the precipitated salted out product, that is, the crude enzyme;

f) Refining: Dissolve the crude enzyme, adjust the pH to 7.0-7.5 with phosphate buffer, filter and stir to collect the filtrate, adjust the pH value to 4.0 with 1M dilute hydrochloric acid, let the enzyme precipitate out, centrifuge to take the precipitate, -80 Freeze-dry at ℃ to get bromelain.

Preferably, in step 2), the moisturizing agent is composed of 20 parts by weight of propylene glycol, 20 parts by weight of glycerin, 10-20 parts by weight of polyethylene glycol, and 0-20 parts by weight of butanediol.

Compared with the prior art, the present invention has the following technical effects:

1. In the present invention, the extracted pure natural bromelain is applied to the skin softening water, so that the skin softening water is more easily absorbed by the skin, and its whitening effect is remarkable;

2. The softening lotion provided by the present invention is generally in the form of a transparent liquid, usually after washing off the dirt adhering to the skin with facial cleanser, etc., it is used to replenish moisture and moisturizing ingredients to the stratum corneum of the skin, so as to make the skin soft and adjust the skin texture. Cosmetics used for physiological purposes;

3. The softening water provided by the present invention has less oil content, has a comfortable feeling, has a good whitening effect, and has a wide range of applications, and is suitable for various skin types;

4. Compared with traditional cosmetics, cosmetics formulated with plant extracts as active ingredients have higher safety performance, and natural components are easier to be absorbed by the skin, which makes the skin rejuvenation and whitening effect of the product more significant.

The present invention will be further described in detail below in combination with specific embodiments.

detailed description

The invention provides a bromelain whitening and softening water, which is made of the following components in parts by weight: 0.5-1 parts by weight of bromelain, 2-10 parts by weight of hyaluronic acid, 50-70 parts by weight of Moisturizing agent, carbomer in 2-20 parts by weight, polyoxyethylene sorbitan monolaurate in 10-20 parts by weight, n-hexyl glucoside in 2-20 parts by weight, triethanolamine in 5-20 parts by weight , 2-10 parts by weight of polyoxyethylene oleyl ether, 1-2 parts by weight of preservatives, 10-20 parts by weight of plant extracts, 5-20 parts by weight of antioxidants and 840-880 parts by weight of deionized water;

Described humectant is any one or its mixture in propylene glycol, butylene glycol, glycerin, polyethylene glycol; Described preservative is methylparaben and/or propylparaben; Described anti- The oxidant is one of tea polyphenols, rosemanol, bamboo leaf flavonoids and lycopene.

In the present invention, bromelain, hyaluronic acid and plant extracts are used as the main effective ingredients for whitening and rejuvenating skin, together with basic moisturizing agent and thickener, and the biological activity of bromelain is promoted by adding n-hexyl glucoside and triethanolamine Solubility and stability of ingredients to prepare stable bromelain whitening and softening lotion with whitening and rejuvenating functions.

The plant extract is any one of rose extract, kiwi fruit extract, lemon extract, papaya extract, and the plant extract has a certain effect of beautifying and moisturizing the skin, lightening dark spots, making the skin fair and tender Effect. These plant extracts work synergistically with bromelain and hyaluronic acid in softening water to whiten and soften the skin.

The preparation method of this bromelain whitening skin softening water comprises the following steps:

1) prepare bromelain: prepare bromelain with pineapple leftovers;

2) Prepare the base solution: Add 2-10 parts by weight of hyaluronic acid into 200 parts by weight of deionized water, heat to 90-95°C, keep the temperature for 20-30min, and keep stirring until the hyaluronic acid is completely dissolved; Then add 2-20 parts by weight of carbomer and 200 parts by weight of deionized water, heat and stir, and keep the temperature of 90-95°C for 60-90min until the carbomer is completely dissolved to obtain the basic solution;

3) Preparation of emulsion: add 50-70 parts by weight of humectant, 2-10 parts by weight of polyoxyethylene oleyl ether, 5-20 parts by weight of triethanolamine, 2-20 parts by weight of Parts of n-hexyl glucoside, 2-10 parts by weight of polyoxyethylene oleyl ether, 1-2 parts by weight of preservatives and the rest of water, heat and stir until all ingredients are completely dissolved and emulsified evenly, and cool to 70-80 ℃, to obtain emulsion;

4) Compounding: Add 10-20 parts by weight of plant extracts and 5-20 parts by weight of antioxidants to the emulsion prepared in step 3), stir evenly, let stand, and wait until the temperature drops to 50°C, add 0.5 - 1 part by weight of bromelain, stirred to dissolve it, and after the bromelain is completely dissolved, let it stand for 24 hours to obtain the bromelain whitening and softening lotion.

In the following examples, the phosphate buffer is a sodium hydroxide solution of potassium dihydrogen phosphate, and the pH of the phosphate buffer is 7.2.

Example 1

The present embodiment provides the preparation method of bromelain, comprises the following steps:

a) Juicing: Wash the pineapple leftovers, squeeze out the juice with a press, and filter to get the filtrate;

Described pineapple leftovers include as pineapple peel, pineapple leaf;

b) Adsorption: adding anhydrous calcium chloride to the filtrate obtained in step 1), stirring and adsorbing, removing the supernatant to obtain an adsorbate;

Take 1L of the filtrate obtained in step a) and add 50 g of anhydrous calcium chloride, stir for adsorption, and remove the supernatant to obtain the adsorbate;

c) Elution: adjust the pH value of the adsorbate to 7.0 with 1M dilute hydrochloric acid, add EDTA for elution, centrifuge, and collect the eluate;

d) Salting out: adjust the pH value of the eluent to 5.0 with 1M dilute hydrochloric acid, add ammonium sulfate powder of 1/4 the mass of the eluent, after it is completely dissolved, salt out at 4°C, and centrifuge to collect the precipitated salted out product, namely Crude enzyme;

f) Refining: Dissolve the crude enzyme, adjust the pH to 7.0-7.5 with phosphate buffer, filter and stir to collect the filtrate, adjust the pH value to 4.0 with 1M dilute hydrochloric acid, let the enzyme precipitate out, centrifuge to take the precipitate, -80 Freeze-dry at ℃ to get bromelain.

Example 2

A preparation method of bromelain whitening and softening water, comprising the following steps:

1) prepare bromelain: adopt the method for embodiment 1 to prepare bromelain from pineapple leftovers;

2) Prepare the basic solution: add 1g of hyaluronic acid to 20g of deionized water, heat to 90-95°C, keep the temperature for 20-30min, and keep stirring until the hyaluronic acid is completely dissolved; then add 0.2g of carbomer 1. 20g deionized water, heat and stir, keep the temperature at 90-95°C for 60-90min, until the carbomer is completely dissolved to obtain the basic solution;

3) Preparation of emulsion: Add 2g propylene glycol, 2g glycerin, 1g polyethylene glycol, 1g polyoxyethylene sorbitan monolaurate, 0.5g triethanolamine, 1g n-hexylglucose to the base solution in step 2). Glycoside, 0.5g polyoxyethylene oleyl ether, 0.05g methylparaben and 0.05g propylparaben, add water to make up to 100g, heat and stir until all ingredients are completely dissolved and emulsified uniformly, cool to 70-80°C, get emulsion;

4) Compounding: Add 1.5g of rose extract and 1g of tea polyphenols to the emulsion prepared in step 3), stir evenly, let stand, wait until the temperature drops to 50°C, add 0.05g of bromelain, stir to make it Dissolve, and after the bromelain is completely dissolved, let it stand for 24 hours to obtain the bromelain whitening and softening lotion.

Example 3

A preparation method of bromelain whitening and softening water, comprising the following steps:

1) prepare bromelain: adopt the method for embodiment 1 to prepare bromelain from pineapple leftovers;

2) Prepare the base solution: Add 1g of hyaluronic acid into 20g of deionized water, heat to 90-95°C, keep the temperature for 20-30min, and keep stirring until the hyaluronic acid is completely dissolved; then add 1g of carbomer, 20g deionized water, heat and stir, keep the temperature at 90-95°C for 60-90min, until the carbomer is completely dissolved to obtain the basic solution;

3) Preparation of emulsion: add 2g propylene glycol, 2g glycerin, 1g polyethylene glycol, 2g polyoxyethylene sorbitan monolaurate, 2g triethanolamine, 2g n-hexyl glucoside to the base solution in step 2) , 0.2g polyoxyethylene oleyl ether and 0.1g methylparaben, add water to make up to 100g, heat and stir until all ingredients are completely dissolved and emulsified evenly, cool to 70-80°C to obtain an emulsion;

4) Compounding: Add 1 g of kiwi fruit extract and 1.5 g of rosmanol to the emulsion prepared in step 3), stir evenly, let stand, wait until the temperature drops to 50°C, add 0.1 g of bromelain, and stir to make it Dissolve, and after the bromelain is completely dissolved, let it stand for 24 hours to obtain the bromelain toner.

Example 4

A preparation method of bromelain whitening and softening water, comprising the following steps:

1) prepare bromelain: adopt the method for embodiment 1 to prepare bromelain from pineapple leftovers;

2) Prepare the base solution: add 0.5g of hyaluronic acid to 20g of deionized water, heat to 90-95°C, keep the temperature for 20-30min, and keep stirring until the hyaluronic acid is completely dissolved; then add 0.5g of Carbopol Carbomer, 20g deionized water, heat and stir, keep the temperature of 90-95°C for 60-90min, until the carbomer is completely dissolved to obtain the basic solution;

3) Preparation of emulsion: add 2g propylene glycol, 2g glycerin, 2g polyethylene glycol, 1g polyoxyethylene sorbitan monolaurate, 2g triethanolamine, 0.5g n-hexylglucose to the base solution in step 2). Glycoside, 0.5g polyoxyethylene oleyl alcohol ether and 0.1g propylparaben, add water to make up to 100g, heat and stir until all ingredients are completely dissolved and emulsified uniformly, cool to 70-80°C to obtain an emulsion;

4) Compounding: Add 2g of lemon extract and 0.5g of bamboo leaf flavonoids to the emulsion prepared in step 3), stir evenly, let it stand still, wait until the temperature drops to 50°C, add 0.08g of bromelain, stir to dissolve After the bromelain is completely dissolved, let it stand for 24 hours to obtain the bromelain toner.

Example 5

A preparation method of bromelain whitening and softening water, comprising the following steps:

1) prepare bromelain: adopt the method for embodiment 1 to prepare bromelain from pineapple leftovers;

2) Prepare the basic solution: Add 0.2g of hyaluronic acid into 20g of deionized water, heat to 90-95°C, keep the temperature for 20-30min, and keep stirring until the hyaluronic acid is completely dissolved; then add 2g of carbomer 1. 20g deionized water, heat and stir, keep the temperature at 90-95°C for 60-90min, until the carbomer is completely dissolved to obtain the basic solution;

3) Preparation of emulsion: Add 2g propylene glycol, 2g butanediol, 2g glycerin, 1g polyethylene glycol, 1g polyoxyethylene sorbitan monolaurate, 1g triethanolamine, 0.2 g to the base solution in step 2). g n-hexyl glucoside, 1g polyoxyethylene oleyl ether and 0.2g methyl paraben, add water to make up to 100g, heat and stir until all ingredients are completely dissolved and emulsified evenly, cool to 70-80°C to obtain an emulsion;

4) Compounding: add 1g of papaya extract and 2g of lycopene to the emulsion prepared in step 3), stir evenly, let stand, wait until the temperature drops to 50°C, add 0.1g of bromelain, stir to dissolve, After the bromelain is completely dissolved, let it stand for 24 hours to obtain the bromelain skin softening water.

Detection example

1. Physical and chemical properties

The physical and chemical properties of the bromelain softening lotion in Examples 2-5 were detected, and the bromelain softening lotion obtained in Example 2 was a transparent liquid without impurities, with a very light rose fragrance and a pH value of 7.2; Example 3 obtained The bromelain softening lotion obtained in Example 4 is a transparent liquid without impurities and has a very light rosemary aroma, and the pH value is 7.0; the bromelain softening lotion obtained in Example 4 is a transparent liquid without impurities and has a very light lemon aroma. The pH value is 7.0; the bromelain skin softening water obtained in Example 5 is a transparent liquid without impurities, has a very light papaya fragrance, and has a pH value of 6.8.

2. Experience test

A total of 12 subjects, numbered 1-12, were all women, and their facial skin had different degrees of pigmentation, dullness, etc., and each used the bromelain toner provided by the invention once in the morning and evening. Among them, 1-3 No. No. uses the product of Example 2, No. 4-6 uses the product of Example 3, No. 7-9 uses the product of Example 4, and No. 10-12 uses the product of Example 5. Clean the skin before use, then pour the product of the present invention on a cotton pad, let the toner fully soak the cotton pad, about 3mL each time, to the extent that the cotton pad is fully soaked without dripping. Gently wipe the skin of the face to achieve the effect of cleansing again. Drop another 1 cent coin-sized lotion onto the palm of your hand, rub your palms to raise the temperature of your palms, press and gently pat the skin on your face to accelerate and promote the penetration of the lotion. One month after the test, the comprehensive effect of the subject's facial skin whitening effect was rated after the test. No effect was grade I, slight effect was grade II, effect was grade III, and effect was significantly grade IV. The results are shown in Table 1,

Table 1 Experience test results

product Example 2 Example 3 Example 4 Example 5 Effect III IV III IV

During the test, the subjects did not experience adverse skin reactions, and the facial skin had different degrees of whitening and lightening of spots.

The above-mentioned embodiment is only a preferred embodiment of the present invention, and cannot be used to limit the protection scope of the present invention. Any insubstantial changes and substitutions made by those skilled in the art on the basis of the present invention belong to the scope of the present invention. Scope of protection claimed.

Claims (5)

1. A bromelain whitening and softening water, is characterized in that, is made up of the following components by weight: the bromelain of 0.5-1 weight part, the hyaluronic acid of 2-10 weight part, 50-70 Moisturizing agent in parts by weight, carbomer in 2-20 parts by weight, polyoxyethylene sorbitan monolaurate in 10-20 parts by weight, n-hexyl glucoside in 2-20 parts by weight, 5-20 parts by weight triethanolamine, 2-10 parts by weight of polyoxyethylene oleyl ether, 1-2 parts by weight of preservatives, 10-20 parts by weight of plant extracts, 5-20 parts by weight of antioxidants and 840-880 parts by weight of deionized water; Described humectant is any one or its mixture in propylene glycol, butylene glycol, glycerin, polyethylene glycol; Described preservative is methylparaben and/or propylparaben; Described anti- The oxidant is one of tea polyphenols, rosemanol, bamboo leaf flavonoids and lycopene; The plant extract is any one of rose extract, kiwi fruit extract, lemon extract and papaya extract. 2. bromelain tender white skin softening water as claimed in claim 1, is characterized in that, described bromelain is made by the following steps: a) Juicing: Wash the pineapple leftovers, squeeze out the juice with a press, and filter to get the filtrate; b) Adsorption: adding anhydrous calcium chloride to the filtrate obtained in step a), stirring and adsorbing, removing the supernatant to obtain the adsorbate; c) Elution: adjust the pH value of the adsorbate to 7.0 with 1M dilute hydrochloric acid, add EDTA for elution, centrifuge, and collect the eluate; d) Salting out: adjust the pH value of the eluent to 5.0 with 1M dilute hydrochloric acid, add ammonium sulfate powder, wait for complete dissolution, salt out at 4°C, and centrifuge to collect the precipitated salted out product, that is, the crude enzyme; f) Refining: Dissolve the crude enzyme, adjust the pH to 7.0-7.5 with phosphate buffer, filter and stir to collect the filtrate, adjust the pH value to 4.0 with 1M dilute hydrochloric acid, let the enzyme precipitate out, centrifuge to take the precipitate, -80 Freeze-dry at ℃ to get bromelain. 3. the preparation method of the bromelain whitening skin softening water as described in any one of claim 1-2, comprises the following steps: 1) prepare bromelain: prepare bromelain with pineapple leftovers; 2) Prepare the base solution: Add 2-10 parts by weight of hyaluronic acid into 200 parts by weight of deionized water, heat to 90-95°C, keep the temperature for 20-30min, and keep stirring until the hyaluronic acid is completely dissolved; Then add 2-20 parts by weight of carbomer and 200 parts by weight of deionized water, heat and stir, and keep the temperature of 90-95°C for 60-90min until the carbomer is completely dissolved to obtain the basic solution; 3) Preparation of emulsion: add 50-70 parts by weight of humectant, 10-20 parts by weight of polyoxyethylene sorbitan monolaurate, and 5-20 parts by weight of triethanolamine to the base solution in step 2) , 2-20 parts by weight of n-hexyl glucoside, 2-10 parts by weight of polyoxyethylene oleyl ether, 1-2 parts by weight of preservatives and the rest of water, heating and stirring until all ingredients are completely dissolved and emulsified evenly, Cool to 70-80°C to obtain emulsion; 4) Compounding: Add 10-20 parts by weight of plant extracts and 5-20 parts by weight of antioxidants to the emulsion prepared in step 3), stir evenly, let stand, and wait until the temperature drops to 50°C, add 0.5 - 1 part by weight of bromelain, stirred to dissolve it, and after the bromelain is completely dissolved, let it stand for 24 hours to obtain the bromelain whitening and softening lotion. 4. method as claimed in claim 3 is characterized in that, step 1) in, preparing bromelain with pineapple leftovers comprises the following steps: a) Juicing: Wash the pineapple leftovers, squeeze out the juice with a press, and filter to get the filtrate; b) Adsorption: adding anhydrous calcium chloride to the filtrate obtained in step a), stirring and adsorbing, removing the supernatant to obtain the adsorbate; c) Elution: adjust the pH value of the adsorbate to 7.0 with 1M dilute hydrochloric acid, add EDTA for elution, centrifuge, and collect the eluate; d) Salting out: adjust the pH value of the eluent to 5.0 with 1M dilute hydrochloric acid, add ammonium sulfate powder, wait for complete dissolution, salt out at 4°C, and centrifuge to collect the precipitated salted out product, that is, the crude enzyme; f) Refining: Dissolve the crude enzyme, adjust the pH to 7.0-7.5 with phosphate buffer, filter and stir to collect the filtrate, adjust the pH value to 4.0 with 1M dilute hydrochloric acid, let the enzyme precipitate out, centrifuge to take the precipitate, -80 Freeze-dry at ℃ to get bromelain. 5. The method according to claim 3, characterized in that, in step 2), the moisturizing agent is composed of 20 parts by weight of propylene glycol, 20 parts by weight of glycerin and polyethylene glycol of 10-20 parts by weight, 0- The butanediol composition of 20 weight parts.