CN105217683B - Application of silver vanadate serving as mimic enzyme - Google Patents

Application of silver vanadate serving as mimic enzyme Download PDF

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CN105217683B
CN105217683B CN201510662605.1A CN201510662605A CN105217683B CN 105217683 B CN105217683 B CN 105217683B CN 201510662605 A CN201510662605 A CN 201510662605A CN 105217683 B CN105217683 B CN 105217683B
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silver vanadate
application
vanadate
solution
silver
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CN105217683A (en
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相振波
张盾
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Institute of Oceanology of CAS
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Institute of Oceanology of CAS
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    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01G—COMPOUNDS CONTAINING METALS NOT COVERED BY SUBCLASSES C01D OR C01F
    • C01G31/00—Compounds of vanadium
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J23/00—Catalysts comprising metals or metal oxides or hydroxides, not provided for in group B01J21/00
    • B01J23/38—Catalysts comprising metals or metal oxides or hydroxides, not provided for in group B01J21/00 of noble metals
    • B01J23/54—Catalysts comprising metals or metal oxides or hydroxides, not provided for in group B01J21/00 of noble metals combined with metals, oxides or hydroxides provided for in groups B01J23/02 - B01J23/36
    • B01J23/66—Silver or gold
    • B01J23/68—Silver or gold with arsenic, antimony, bismuth, vanadium, niobium, tantalum, polonium, chromium, molybdenum, tungsten, manganese, technetium or rhenium
    • B01J23/682—Silver or gold with arsenic, antimony, bismuth, vanadium, niobium, tantalum, polonium, chromium, molybdenum, tungsten, manganese, technetium or rhenium with vanadium, niobium, tantalum or polonium
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2523/00—Constitutive chemical elements of heterogeneous catalysts
    • B01J2523/10—Constitutive chemical elements of heterogeneous catalysts of Group I (IA or IB) of the Periodic Table
    • B01J2523/18—Silver
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2523/00—Constitutive chemical elements of heterogeneous catalysts
    • B01J2523/50—Constitutive chemical elements of heterogeneous catalysts of Group V (VA or VB) of the Periodic Table
    • B01J2523/55—Vanadium
    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01P—INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS
    • C01P2002/00—Crystal-structural characteristics
    • C01P2002/70—Crystal-structural characteristics defined by measured X-ray, neutron or electron diffraction data
    • C01P2002/72—Crystal-structural characteristics defined by measured X-ray, neutron or electron diffraction data by d-values or two theta-values, e.g. as X-ray diagram
    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01P—INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS
    • C01P2004/00—Particle morphology
    • C01P2004/01—Particle morphology depicted by an image
    • C01P2004/03—Particle morphology depicted by an image obtained by SEM
    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01P—INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS
    • C01P2004/00—Particle morphology
    • C01P2004/01—Particle morphology depicted by an image
    • C01P2004/04—Particle morphology depicted by an image obtained by TEM, STEM, STM or AFM
    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01P—INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS
    • C01P2004/00—Particle morphology
    • C01P2004/10—Particle morphology extending in one dimension, e.g. needle-like
    • C01P2004/17—Nanostrips, nanoribbons or nanobelts, i.e. solid nanofibres with two significantly differing dimensions between 1-100 nanometer

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Materials Engineering (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Inorganic Chemistry (AREA)
  • Inorganic Compounds Of Heavy Metals (AREA)
  • Catalysts (AREA)

Abstract

The invention relates to a technology of mimic enzymes, in particular to an application of silver vanadate serving as a mimic enzyme. According to the application of silver vanadate serving as the mimic enzyme, silver vanadate is obtained through hydro-thermal synthesis. Silver vanadate has excellent HRP (horseradish peroxidase) catalytic activity, has potential application prospect in the fields such as immunoassay and the like and is cheap and easy to obtain.

Description

A kind of silver vanadate is as the application of analogue enztme
Technical field
The present invention relates to analogue enztme technology, a kind of silver vanadate is as the application of analogue enztme.
Background technology
Peroxidase (Horseradish peroxidase is called for short HRP) is by microorganism or plant A produced class oxidoreductase, is the enzyme aoxidized for electron acceptor catalytic substrate with hydrogen peroxide. HRP is one of toolenzyme the most commonly used in immunoenzymatic technique, is usually used in traget antibody.
Document (Nature Nanotechnology, 2007,2,577-583) reported first Fe3O4 Magnetic nanoparticle have HRP catalysis activity, the specificity of its catalytic efficiency, mechanism and substrate all with HRP is identical.And compare with HRP, also have that stability is high, preparation technology simple and reusable edible Advantage.At Fe3O4Afterwards, graphene oxide, CNT, cerium oxide, oxygen are the most in succession reported Change the nano materials such as manganese and also there is HRP catalysis activity, obtain at immunoassay and field of biosensors Extensively quote.
Silver vanadate, due to electronics, photon and the magnetism characteristic of its uniqueness, has become as research in recent years Focus.Silver vanadate has the crystal structure of uniqueness, and it is not yet developed at the application potential of biological field.
Summary of the invention
It is an object of the invention to provide the application as analogue enztme of a kind of silver vanadate.
For achieving the above object, the technical solution used in the present invention is:
A kind of silver vanadate is as the application of analogue enztme, and described silver vanadate is as the application of analogue enztme.
Described silver vanadate is as the HRP enzyme that substrate carries out catalytic oxidation-reduction reaction.
Described silver vanadate is obtained by Hydrothermal Synthesis, and for a length of 0.5 to several microns, width is 100 About nm ribbon.
Described silver vanadate is by AgNO3Aqueous solution drops to NH4VO3Aqueous solution in, drip ammonia the most again, Regulation pH value of solution is 5-9；Solution crystallization 24-48 hour under the conditions of 160-200 DEG C after regulation, brilliant Wash after change, filter, filter cake is dried 24 hours at 40-60 DEG C, obtains AgVO3。
Specifically:
1) by NH4VO3It is configured to the aqueous solution that concentration is 0.033mol/L, then by AgNO3It is configured to Concentration is the aqueous solution of 0.067mol/L, AgNO3Solution is added dropwise to NH4VO3In solution, subsequently Being added dropwise over ammonia again, regulation pH value of solution is 7；
2) by above-mentioned mixed solution crystallization 24-48 hour under the conditions of 160-200 DEG C, after crystallization successively By deionized water, washing with alcohol, filter, filter cake is dried 24 hours at 40-60 DEG C, obtains chemistry Consist of AgVO3Silver vanadate analogue enztme material.
The had the advantages that gained analogue enztme material of the present invention of the present invention has good HRP enzyme Catalysis activity, has potential application foreground in fields such as immunoassays, and cheap and easy to get.
Accompanying drawing explanation
XRD spectra (wherein abscissa-the angle of the analogue enztme material that Fig. 1 provides for the embodiment of the present invention Spending 2 θ, unit is ° (spending)；Vertical coordinate-intensity, unit is a.u. (absolute unit)).
TEM and the SEM figure of the analogue enztme material that Fig. 2 provides for the embodiment of the present invention.
The analogue enztme material that Fig. 3 provides for embodiment of the present invention color change (A) in different systems With ultraviolet-visible absorption spectroscopy (B) (a:H2O2+AgVO3；b:TMB+AgVO3；c:TMB+H2O2；d: TMB+H2O2+AgVO3；TMB:3,3', 5,5'-tetramethyl benzidine, for the typical peroxidase of one Catalytic substrate)
Detailed description of the invention
Embodiment 1
Silver vanadate preparation process:
By NH4VO3It is configured to the aqueous solution that concentration is 0.033mol/L, then by AgNO3It is configured to concentration For the aqueous solution of 0.067mol/L, AgNO3Solution is added dropwise to NH4VO3In solution, the most again by Being added dropwise to ammonia, regulation pH value of solution is 7；By above-mentioned mixed solution crystallization under the conditions of 160-200 DEG C 24-48 hour, successively by deionized water, washing with alcohol after crystallization, filter, by filter cake at 40-60 DEG C Being dried 24 hours, obtaining chemical composition is AgVO3Silver vanadate (Fig. 1 and 2).
Being a length of 0.5 to several microns by product synthesized seen from Fig. 1 and 2, width is 100nm The banding silver vanadate of left and right Nano grade.
Embodiment 2
Utilize typical catalysis oxidation substrates TMB and H2O2Measure the class peroxide of the silver vanadate of synthesis Enzymatic activity.
The silver vanadate Mimetic enzyme effect to substrate TMB: take a little centrifuge tube, adds 800 μ L phosphate buffer (100mM, pH 5.0), is then respectively adding 8mM TMB and 5mM H2O2 As substrate, it is eventually adding 300 μ g mL-1AgVO3Nano belt (nanobelts).Work as AgVO3 After nanobelts joins reaction system, it is possible to the system that detects by an unaided eye color changes.All of reaction System absorbance all passes through Hitachi U-2900 type ultraviolet spectrophotometer and uses time sweep pattern at wavelength Carry out under the conditions of 652nm detecting (seeing Fig. 3).
By Fig. 3 (A), it can be observed that TMB solution colour change under different system catalysis, (a)H2O2+AgVO3Nanobelts system, solution water white transparency, (b) TMB+AgVO3nanobelts System, solution water white transparency, (c) TMB+H2O2System, solution water white transparency, (d) H2O2+TMB+AgVO3 Nanobelts system, solution colour has obvious blueness, illustrates that silver vanadate catalysis TMB generates chemistry Reaction.Fig. 3 (B) is the ultraviolet-visible absorption spectroscopy figure that three kinds of reaction systems are corresponding, and 652nm is oxidation The characteristic absorption wavelength of state TMB, system a-c is at 652nm almost without absworption peak, and system d is also It is exactly H2O2+TMB+AgVO3Nanobelts system has obvious absworption peak, and peak at 652nm Shape is preferable；Illustrate that silver vanadate catalysis TMB generates the TMB of oxidation state.Conclusion as can be drawn from Figure 3: vanadium Acid silver can be catalyzed TMB and H2O2Reaction generates blue oxide state TMB, illustrates that silver vanadate has class peroxide Compound enzymatic activity.

Claims (3)

1. a silver vanadate is as the application of analogue enztme, it is characterised in that:
Described silver vanadate is as the HRP enzyme that substrate carries out catalytic oxidation-reduction reaction；Described silver vanadate For AgVO3。
2. the silver vanadate as described in claim 1 is as the application of analogue enztme, it is characterised in that: described Silver vanadate is obtained by Hydrothermal Synthesis.
3. the silver vanadate as described in claim 2 is as the application of analogue enztme, it is characterised in that: described The preparation method of silver vanadate is: by AgNO3Aqueous solution drops to NH4VO3Aqueous solution in, drip the most again Adding ammonia, regulation pH value of solution is 5-9；Solution crystallization 24-48 under the conditions of 160-200 DEG C after regulation Hour, wash after crystallization, filter, filter cake is dried 24 hours at 40-60 DEG C, obtains AgVO3。
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Publication number Priority date Publication date Assignee Title
CN106219606B (en) * 2016-07-12 2018-07-10 陕西科技大学 A kind of nanometer of flower ball-shaped Ag3VO4Preparation method
CN107376911A (en) * 2017-07-27 2017-11-24 中国科学院海洋研究所 A kind of application of α silver vanadates as analogue enztme
CN109420497A (en) * 2017-08-21 2019-03-05 中国科学院海洋研究所 A kind of InVO4-AgVO3 hetero-junctions catalyst and its preparation method and application
CN111439783B (en) * 2020-03-07 2023-02-21 温州医科大学 Silver vanadate, preparation method thereof and application of silver vanadate as peroxidase-like enzyme to detection of uric acid
CN114441458B (en) * 2021-05-24 2023-06-09 中国科学院海洋研究所 Application of ZIF material in inhibition of mimic enzyme
CN113277558B (en) * 2021-06-17 2022-05-17 湘潭大学 Alpha' -AgVO3Preparation method of nano material
CN115490264B (en) * 2022-09-15 2023-10-20 山东黄海科技创新研究院 Preparation method of silver vanadate mimic enzyme and antibacterial application of silver vanadate mimic enzyme

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