CN105213462A - A kind of can the preparation method extracting total saponins in Radix Platycodonis of suitability for industrialized production - Google Patents
A kind of can the preparation method extracting total saponins in Radix Platycodonis of suitability for industrialized production Download PDFInfo
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- CN105213462A CN105213462A CN201510602440.9A CN201510602440A CN105213462A CN 105213462 A CN105213462 A CN 105213462A CN 201510602440 A CN201510602440 A CN 201510602440A CN 105213462 A CN105213462 A CN 105213462A
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- exchange column
- macroporous type
- total saponins
- basic anion
- radix platycodonis
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Abstract
The invention discloses a kind of can the preparation method extracting total saponins in Radix Platycodonis of suitability for industrialized production, comprise following concrete technology step: 1) get Radix Platycodonis medical material, being shattered by Radix Platycodonis medical material is 10 ~ 100 object powder; 2) Platycodon Root of step 1) gained is added water or 50 ~ 85% ethanol, extract again after solution being adjusted to pH value 10 ~ 12, obtain extracting solution; 3) macroporous type basic anion exchange column on extracting solution, first use aqueous alkali and pure water rinsing, flushing liquor discards, then uses 50 ~ 85% ethanol elutions, and eluent concentrates, and obtains Radix Platycodi total saponins; Employing the preparation mode of suitability for industrialized production can carry out the extraction preparation of Radix Platycodi total saponins, effectively can improve the income rate of extraction efficiency and total saponins.
Description
Technical field
The present invention relates to biological pharmacy technical field, specifically, be a kind of can the preparation method extracting total saponins in Radix Platycodonis of suitability for industrialized production.
Background technology
Radix Platycodonis (BalloonFlower), another name burden flower, Radix Platycodonis, mitral flower, be herbaceos perennial, stem is high 20 ~ 120 centimetres, usually without hair, even close by undercoat, not branch, few top branch.Leaf is all verticillate, and part is verticillate to whole alternate, stockless or have extremely short handle, and blade is avette, and ovum shape is oval to lanceolar, the avette or ovum shape lanceolar of leaf, and flower skipper or dark violet white, can do ornamental flower; Its root can be used as medicine, and has the effect such as eliminating phlegm and stopping cough, lung qi dispersing, evacuation of pus, traditional Chinese medical science common medicine.Often pickled as brined vegetable in Northeast Area of China, be used in the Korea peninsula make Pickles, what local folk rhyme " Radix Platycodonis ballad " was described is exactly this kind of plant.Radix Platycodonis is the dry root of campanulaceae (Campanulaceae) Platycodon grandiflouorum Platycodongrandiflorum (Jacq.) A.DC., for tradition commonly uses one of antisussive and expectorant agent.Modern pharmacology research shows: Radix Platycodonis has the wide applications such as eliminating phlegm and relieving cough, antiinflammatory, gastric acid secretion inhibiting and anti-gastric-ulcer, calmness, antipyretic, analgesia, blood sugar lowering, blood fat reducing and cholesterol reducing, thus core be applied to various prescription and clinical in.In addition Radix Platycodonis is again medicinal and edible plant. so market demand is very large.The main active of Radix Platycodonis is platycodin, and traditional platycodin preparation method cost is high, need consume the reagent such as a large amount of acetone and ether, complicated operation, be unfavorable for suitability for industrialized production, be therefore necessary to make it the extraction separation method that searching one is new to be suitable for suitability for industrialized production.
Summary of the invention
The object of the invention is to design a kind of can the preparation method extracting total saponins in Radix Platycodonis of suitability for industrialized production, adopt and the preparation mode of suitability for industrialized production can carry out the extraction preparation of Radix Platycodi total saponins, effectively can improve the income rate of extraction efficiency and total saponins.
The present invention is achieved through the following technical solutions: a kind of can the preparation method extracting total saponins in Radix Platycodonis of suitability for industrialized production, comprise following concrete technology step:
1) get Radix Platycodonis medical material, being shattered by Radix Platycodonis medical material is 10 ~ 100 object powder;
2) Platycodon Root of step 1) gained is added water or 50 ~ 85% ethanol, extract again after solution being adjusted to pH value 10 ~ 12, obtain extracting solution;
3) macroporous type basic anion exchange column on extracting solution, first use aqueous alkali and pure water rinsing, flushing liquor discards, then uses 50 ~ 85% ethanol elutions, and eluent concentrates, and obtains Radix Platycodi total saponins;
Wherein, step 2) described macroporous type basic anion exchange column be selected from macroporous type weakly-basic anion D392 exchange column, macroporous type weakly-basic anion D380 exchange column, macroporous type weakly-basic anion D382 exchange column, macroporous type weakly-basic anion D371 exchange column or macroporous type strong alkalinity anion D201 exchange column one or more; Described aqueous alkali is selected from 2%NaOH, 2%KOH or 2%Na
2cO
3.
For better realizing the present invention further, in described step 1), adopt NaOH, KOH, Na
2cO
3or NaHCO
3regulate solution ph.
For better realizing the present invention further, the preparation technology of extracting solution described in described step 1) is:
1-1) joined by Platycodon Root in water or 50 ~ 85% ethanol, obtain solution, the ratio of described powder and water or 50 ~ 85% ethanol is 1:3 ~ 5 by weight;
1-2) solution is adjusted to the alkaline solution of pH value 10 ~ 12, by alkaline solution heating or supersound extraction 2 ~ 4 times, merge extractive liquid, filters; The further remove impurity of filtrate, aqueous extract alcohol precipitating method remove impurity, alcohol extract then uses the remove impurity of water precipitating method, obtains the clear liquor after remove impurity.
For better realizing the present invention further, the concrete technology of described step 3) is:
One in macroporous type weakly-basic anion D392 exchange column, macroporous type weakly-basic anion D380 exchange column, macroporous type weakly-basic anion D382 exchange column, macroporous type weakly-basic anion D371 exchange column or macroporous type strong alkalinity anion D201 exchange column on clear liquor is the more planted, first uses 2%NaOH, 2%KOH or 2%Na
2cO
3rinse, then use pure water rinsing, flushing liquor discards, and then uses 50 ~ 85% ethanol elutions, and eluent concentrates, dry, obtains Radix Platycodi total saponins.
The present invention compared with prior art, has the following advantages and beneficial effect:
The present invention adopts and the preparation mode of suitability for industrialized production can carry out the extraction preparation of Radix Platycodi total saponins, effectively can improve the income rate of extraction efficiency and total saponins.
It is high that application of the present invention has separation efficiency, and Radix Platycodi total saponins yield high, gained Radix Platycodi total saponins can keep higher physiologically active, and is easy to industrialized great production.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
Embodiment 1:
Can the preparation method extracting total saponins in Radix Platycodonis of suitability for industrialized production, comprise following concrete technology step:
1) get Radix Platycodonis medical material, being shattered by Radix Platycodonis medical material is 10 ~ 100 object powder;
2) Platycodon Root of step 1) gained is added water or 50 ~ 85% ethanol, extract again after solution being adjusted to pH value 10 ~ 12, obtain extracting solution;
3) macroporous type basic anion exchange column on extracting solution, first use aqueous alkali and pure water rinsing, flushing liquor discards, then uses 50 ~ 85% ethanol elutions, and eluent concentrates, and obtains Radix Platycodi total saponins;
Wherein, step 2) described macroporous type basic anion exchange column be selected from macroporous type weakly-basic anion D392 exchange column, macroporous type weakly-basic anion D380 exchange column, macroporous type weakly-basic anion D382 exchange column, macroporous type weakly-basic anion D371 exchange column or macroporous type strong alkalinity anion D201 exchange column one or more; Described aqueous alkali is selected from 2%NaOH, 2%KOH or 2%Na
2cO
3.
Embodiment 2:
The present embodiment is at the enterprising one-step optimization in the basis of above-described embodiment, for better realizing the present invention further, in described step 1), adopts NaOH, KOH, Na
2cO
3or NaHCO
3regulate solution ph.
Embodiment 3:
The present embodiment is at the enterprising one-step optimization in the basis of above-mentioned any embodiment, and for better realizing the present invention further, the preparation technology of extracting solution described in described step 1) is:
1-1) joined by Platycodon Root in water or 50 ~ 85% ethanol, obtain solution, the ratio of described powder and water or 50 ~ 85% ethanol is 1:3 ~ 5 by weight;
1-2) solution is adjusted to the alkaline solution of pH value 10 ~ 12, by alkaline solution heating or supersound extraction 2 ~ 4 times, merge extractive liquid, filters; The further remove impurity of filtrate, aqueous extract alcohol precipitating method remove impurity, alcohol extract then uses the remove impurity of water precipitating method, obtains the clear liquor after remove impurity.
Embodiment 4:
The present embodiment is at the enterprising one-step optimization in the basis of above-described embodiment, and for better realizing the present invention further, the concrete technology of described step 3) is:
One in macroporous type weakly-basic anion D392 exchange column, macroporous type weakly-basic anion D380 exchange column, macroporous type weakly-basic anion D382 exchange column, macroporous type weakly-basic anion D371 exchange column or macroporous type strong alkalinity anion D201 exchange column on clear liquor is the more planted, first uses 2%NaOH, 2%KOH or 2%Na
2cO
3rinse, then use pure water rinsing, flushing liquor discards, and then uses 50 ~ 85% ethanol elutions, and eluent concentrates, dry, obtains Radix Platycodi total saponins.
The above is only preferred embodiment of the present invention, and not do any pro forma restriction to the present invention, every any simple modification, equivalent variations done above embodiment according to technical spirit of the present invention, all falls within protection scope of the present invention.
Claims (4)
1. can the preparation method extracting total saponins in Radix Platycodonis of suitability for industrialized production, it is characterized in that: comprise following concrete technology step:
1) get Radix Platycodonis medical material, being shattered by Radix Platycodonis medical material is 10 ~ 100 object powder;
2) Platycodon Root of step 1) gained is added water or 50 ~ 85% ethanol, extract again after solution being adjusted to pH value 10 ~ 12, obtain extracting solution;
3) macroporous type basic anion exchange column on extracting solution, first use aqueous alkali and pure water rinsing, flushing liquor discards, then uses 50 ~ 85% ethanol elutions, and eluent concentrates, and obtains Radix Platycodi total saponins;
Wherein, step 2) described macroporous type basic anion exchange column be selected from macroporous type weakly-basic anion D392 exchange column, macroporous type weakly-basic anion D380 exchange column, macroporous type weakly-basic anion D382 exchange column, macroporous type weakly-basic anion D371 exchange column or macroporous type strong alkalinity anion D201 exchange column one or more; Described aqueous alkali is selected from 2%NaOH, 2%KOH or 2%Na
2cO
3.
2. according to claim 1 a kind of can the preparation method extracting total saponins in Radix Platycodonis of suitability for industrialized production, it is characterized in that: in described step 1), adopt NaOH, KOH, Na
2cO
3or NaHCO
3regulate solution ph.
3. according to claim 1 and 2 a kind of can the preparation method extracting total saponins in Radix Platycodonis of suitability for industrialized production, it is characterized in that: the preparation technology of extracting solution described in described step 1) is:
1-1) joined by Platycodon Root in water or 50 ~ 85% ethanol, obtain solution, the ratio of described powder and water or 50 ~ 85% ethanol is 1:3 ~ 5 by weight;
1-2) solution is adjusted to the alkaline solution of pH value 10 ~ 12, by alkaline solution heating or supersound extraction 2 ~ 4 times, merge extractive liquid, filters; The further remove impurity of filtrate, aqueous extract alcohol precipitating method remove impurity, alcohol extract then uses the remove impurity of water precipitating method, obtains the clear liquor after remove impurity.
4. according to claim 3 a kind of can the preparation method extracting total saponins in Radix Platycodonis of suitability for industrialized production, it is characterized in that: the concrete technology of described step 3) is:
One in macroporous type weakly-basic anion D392 exchange column, macroporous type weakly-basic anion D380 exchange column, macroporous type weakly-basic anion D382 exchange column, macroporous type weakly-basic anion D371 exchange column or macroporous type strong alkalinity anion D201 exchange column on clear liquor is the more planted, first uses 2%NaOH, 2%KOH or 2%Na
2cO
3rinse, then use pure water rinsing, flushing liquor discards, and then uses 50 ~ 85% ethanol elutions, and eluent concentrates, dry, obtains Radix Platycodi total saponins.
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|---|---|---|---|
| CN201510602440.9A CN105213462A (en) | 2015-09-21 | 2015-09-21 | A kind of can the preparation method extracting total saponins in Radix Platycodonis of suitability for industrialized production |
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|---|---|---|---|
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101084971A (en) * | 2006-06-08 | 2007-12-12 | 天津天士力之骄药业有限公司 | Method for preparing total saponins of platycodon grandiflorum |
| CN102327310A (en) * | 2011-11-04 | 2012-01-25 | 安徽中医学院 | Preparation method of high-purity platycodon root total saponin |
-
2015
- 2015-09-21 CN CN201510602440.9A patent/CN105213462A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101084971A (en) * | 2006-06-08 | 2007-12-12 | 天津天士力之骄药业有限公司 | Method for preparing total saponins of platycodon grandiflorum |
| CN102327310A (en) * | 2011-11-04 | 2012-01-25 | 安徽中医学院 | Preparation method of high-purity platycodon root total saponin |
Non-Patent Citations (1)
| Title |
|---|
| 《吉林农业大学学报》 * |
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Application publication date: 20160106 |