CN105211555A - One utilizes edible fungi residue fermenting and producing cow protein feed new method - Google Patents
One utilizes edible fungi residue fermenting and producing cow protein feed new method Download PDFInfo
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Abstract
The present invention relates to one and utilize edible fungi residue fermenting and producing cow protein feed new method, comprise the steps: the preparation of (1) seed liquor: by saccharomycete 1, Aspergillus 1, lactic acid bacteria 1, in the culture medium comprising carbon source, nitrogenous source and limiting nutrient source, carry out expansion respectively cultivate, obtain seed liquor; (2) process of edible fungi residue: get dry mushroom bacterium slag 30-40 part, water 45-55 part, wheat bran 1-5 part, sugared 0.5-1 part (wet bacteria slag does not need to add water), stirs after mixing; (3) be inoculated in edible fungi residue by the seed liquor that step (1) obtains, total inoculum concentration is 5-10%, and at 35 DEG C, quiescent culture 5-7d, to obtain final product.Utilizing microorganism to ferment to edible fungi residue makes the protein content of bacterium slag be significantly improved, and make crude fibre change into the fiber that can be utilized by milk cow, make the waste material in Edible Fungi obtain good utilization, create corresponding economic worth and resource is obtained and well recycle.
Description
Technical field
The invention belongs to biological technical field, particularly one utilizes edible fungi residue fermenting and producing cow protein feed new method.
Background technology
China is the country of protein feed resources shortage, the annual breach of current protein feed about 1,500 ten thousand tons, therefore the industry of China's development protein feed must walk the road of applicable China's national situation, and namely select cheap, the raw material of wide material sources and the technique of low cost produce protein feed.
As Edible Fungi big country, national edible mushroom total output in 2013 reaches 3.21 × 10 according to statistics
7ton, the output value is more than 1,400 hundred million.Developing rapidly along with mushroom industry, solid medium discarded after fruit body of edible fungi of gathering, namely the quantity of bacterium slag gets more and more.Investigation display is often produced 1kg edible mushroom and is about producing strains slag quantity and reaches 3kg.But how effective utilization is carried out to the bacterium slag of these suitability for industrialized production and be never well solved, bacterium slags a large amount of is every year dropped and is even burned, and causes certain waste and environmental pollution.Measure according to relevant department, crude protein 10.2% is contained, crude fibre 9.32%, crude fat 1.2%, calcium 3.2%, phosphorus 2.1%, lysine 1.2%, tryptophan 0.8%, methionine plus cystine 1.2% in the bacterium slag particle face of producing country edible mushroom.Therefore, it is possible to utilize edible fungi residue as the raw materials for production of protein feed, be significant.
Summary of the invention:
For the problems referred to above, the present invention utilizes microorganism to ferment to edible fungi residue the protein content of bacterium slag is significantly improved again, and make crude fibre change into the fiber that can be utilized by milk cow, make the waste material in Edible Fungi obtain good utilization, create corresponding economic worth and resource is obtained and well recycle.The method has simply, efficient, the processing time is short, applied widely, alternate is mutually beneficial between bacterial classification, mutually the advantage of not antagonism
To achieve these goals, the present invention adopts following technical scheme:
One utilizes edible fungi residue fermenting and producing cow protein feed new method, candida utili, aspergillus albicans, Lactobacillus plantarum is adopted to carry out mixed culture fermentation to edible fungi residue, crude protein in bacterium slag, crude fibre are changed into further by the nutriment of milk cow digestibility and utilization, to obtain final product.
Preferably, described edible mushroom be flat mushroom, one or more in pleurotus eryngii, asparagus or white beech mushroom.
Above-mentioned method specifically comprises the steps:
(1) preparation of primary seed solution: by candida utili, aspergillus albicans, Lactobacillus plantarum, carries out expansion respectively and cultivates, obtain seed liquor;
(2) preparation of secondary seed solution: the primary seed solution of candida utili, aspergillus albicans, Lactobacillus plantarum is inoculated in maize powder medium according to 1-3%:1-3%:2-5%, first aerobic cultivation 18h, then static gas wave refrigerator 24h, is secondary seed solution.
The process of edible fungi residue: get dry mushroom bacterium slag 30-40 part, water 45-55 part, wheat bran 1-5 part, sugared 0.5-1 part, stirs after mixing;
(3) be inoculated in edible fungi residue by the secondary seed solution that step (2) obtains, total inoculum concentration is 5-10%, and at 35 DEG C, quiescent culture 5-7d, to obtain final product.
In step (1), the culture medium of described candida utili bacterium and aspergillus candidus is murphy juice fluid nutrient medium: potato 200g, and add water 1000ml, boiling water boiling half an hour, filters and adds 20g sucrose, 121 DEG C of sterilizing 20min.
The modified culture medium of Lactobacillus plantarum is MRS culture medium: peptone 5g, yeast extract 5g, dipotassium hydrogen phosphate 2.3g, sodium acetate 2g, lactose 2g, glucose 10g, corn flour 10g, epsom salt 0.5g, manganese sulfate 0.2g, water 1000ml, regulate PH6.2-6.4,121 DEG C of sterilizing 20min.
Maize powder medium in step (2) is: corn flour 20g (crossing 80 mesh sieves), brown sugar 10g, lactose 1g, beancake powder 5g, water 1000ml, 121 DEG C of sterilizing 20min.
Protein feed prepared by said method is feeding the application in milk cow.
The effect that the present invention is useful is:
1. the present invention utilizes waste material of edible mushroom fermentation production of protein feedstuff, and domestic research in this is also few.This patent mainly utilizes microorganism to ferment to edible fungi residue the protein content of bacterium slag is significantly improved, and make crude fibre change into the fiber that can be utilized by milk cow, make the waste material in Edible Fungi obtain good utilization, create corresponding economic worth and resource is obtained and well recycle
2. in the present invention, before and after fermentation, protein content is significantly improved, and unavailable cellulose is significantly declined, and crude fat also declines to some extent, improves like this have great importance for feed quality
Detailed description of the invention
The present invention will be further elaborated below.Should be noted that following explanation is only to explain the present invention, its content not being limited.
Embodiment 1:
Below by instantiation, the present invention will be further elaborated, should be noted that following explanation is only to explain the present invention, not limiting its content.
1. fermentation bacterial strain:
Aspergillus albicans (Aspergilluscandidus) is the bacterial strain being numbered CICC40217 of Chinese industrial Microbiological Culture Collection administrative center; Be rich in cellulase, carbohydrase and protease.
Candida utili (Candidautilis) is the bacterial strain being numbered CICC1314 of Chinese industrial Microbiological Culture Collection administrative center; Thalline be good protein, Cobastab content high, pentose and hexose can be utilized.
Lactobacillus plantarum (lactobacillusplantarum) is the bacterial strain being numbered CICC20764 of Chinese industrial Microbiological Culture Collection administrative center; Tool is anticorrosion, promote absorption of nutrient ingredients, maintain intestinal flora balanced action.
2. culture medium:
Murphy juice fluid nutrient medium: potato 200g, add water 1000ml, boiling water boiling half an hour, filters and add 20g sucrose, 121 DEG C of sterilizing 20min.
Modified MRS culture medium is culture medium: peptone 5g, yeast extract 5g, dipotassium hydrogen phosphate 2.3g, sodium acetate 2g, lactose 2g, glucose 10g, corn flour 10g, epsom salt 0.5g, manganese sulfate 0.2g, water 1000ml, regulate PH6.2-6.4,121 DEG C of sterilizing 20min.
Maize powder medium is: corn flour 20g (crossing 80 mesh sieves), brown sugar 10g, lactose 1g, beancake powder 5g, water 1000ml, 121 DEG C of sterilizing 20min.
3. edible fungi residue: suitability for industrialized production flat mushroom, pleurotus eryngii, asparagus, white beech mushroom discarded (dry, wet) bacterium slag
4.3,5-dinitrosalicylic acid
5. preparation method
(1) preparation of primary seed solution: by candida utili bacterium, aspergillus candidus, Lactobacillus plantarum, carries out expansion respectively and cultivates, obtain seed liquor in liquid PDA and MRS culture medium;
(2) preparation of secondary seed solution: the primary seed solution of candida utili bacterium, aspergillus candidus, Lactobacillus plantarum is inoculated in maize powder medium according to 1%:1%:2%, first aerobic cultivation 18h, then static gas wave refrigerator 24h, is secondary seed solution.
The process of edible fungi residue: get dry mushroom bacterium slag 45 parts, 45 parts, water, 1 part, wheat bran, sugar 0.5 part, stirs after mixing;
(3) be inoculated in edible fungi residue by the secondary seed solution that step (2) obtains, total inoculum concentration is 5%, and at 35 DEG C, quiescent culture 5-7d, to obtain final product.
(4) determining the protein quantity in bacterium slag: micro-Kjeldahl, hemicellulose enzyme activity determination: DNS method, crude fat content measures: soxhlet extraction methods, and amylase activity measures: 3,5-dinitrosalicylic acid system
(5) milk cow feeding trial: from cattle farm, select body weight, age, parity, the lactation moon, lactating cow 20 that daily milk yield is basically identical or close with physiological status, be divided into two groups at random, often organize 10, it is test group (zymophyte slag content is 25%) that basal diet of feeding adds a group of fermentation mushroom bacteria chaff, what feed former basal diet is control group, measures its body weight and milk production.
Embodiment 2: multi-functional bacterium compoiste fermented protease feed index of correlation is surveyed and detected
Edible fungi residue prepared by the embodiment of the present invention 1 carry out the crude protein of zymophyte slag again, crude fat, crude fibre, etc. index detect and milk cow feeding trial with unfermentable feed as a comparison, the results are shown in Table 1-4.
Determining the protein quantity in bacterium slag before and after table 1 ferments
Bacterium slag crude fiber content before and after table 2 ferments
Bacterium slag crude fat content before and after table 3 ferments
Be significantly improved by table 1,2, the 3 front and back protein content that can ferment, unavailable cellulose is significantly declined, and crude fat also declines to some extent, improves like this have great importance for feed quality.
Table 4 is fed fermented feed and is contrasted with basal diet and basal diet galactopoiesis
Can test combination control group output of milk difference by table 4 little, and the experimental group output of milk and body weight evolution want a little higher than control group, therefore replace feed a part to have feasibility by the edible fungi residue after fermenting.
Embodiment 2
Treatment step is identical with embodiment 1, and difference is, described edible mushroom is flat mushroom.
Embodiment 3
Treatment step is identical with embodiment 1, and difference is, described edible mushroom is pleurotus eryngii.
Embodiment 4
Treatment step is identical with embodiment 1, and difference is, described edible mushroom is asparagus.
Embodiment 5
Described edible mushroom is white beech mushroom.
Embodiment 6
Treatment step is identical with embodiment 1, difference is, in step (2), the preparation of secondary seed solution: the primary seed solution of candida utili bacterium, aspergillus candidus, Lactobacillus plantarum is inoculated in maize powder medium according to 2%:2%:4%, first aerobic cultivation 18h, then static gas wave refrigerator 24h, is secondary seed solution.
Embodiment 7
Treatment step is identical with embodiment 1, difference is, in step (2), the preparation of secondary seed solution: the primary seed solution of candida utili bacterium, aspergillus candidus, Lactobacillus plantarum is inoculated in maize powder medium according to 3%:3%:5%, first aerobic cultivation 18h, then static gas wave refrigerator 24h, is secondary seed solution.
Embodiment 8
Treatment step is identical with embodiment 1, and difference is, in step (2), and the process of edible fungi residue: get dry mushroom bacterium slag 35 parts, 50 parts, water, 3 parts, wheat bran, sugar 0.8 part, stirs after mixing;
Embodiment 9
Treatment step is identical with embodiment 1, and difference is, in step (2), and the process of edible fungi residue: get dry mushroom bacterium slag 40 parts, 55 parts, water, 5 parts, wheat bran, sugar 1 part, stirs after mixing;
Embodiment 10
Treatment step is identical with embodiment 1, and difference is, in step (3), be inoculated in edible fungi residue by the secondary seed solution that step (2) obtains, total inoculum concentration is 8%, and at 35 DEG C, quiescent culture 5-7d, to obtain final product.
Embodiment 11
Treatment step is identical with embodiment 1, and difference is, in step (3), be inoculated in edible fungi residue by the secondary seed solution that step (2) obtains, total inoculum concentration is 10%, and at 35 DEG C, quiescent culture 5-7d, to obtain final product.
The feed adopting the method for embodiment of the present invention 3-11 to produce all can meet the standard-required of cow protein feed.
Although above-mentioned, the specific embodiment of the present invention is described; but not limiting the scope of the invention; one of ordinary skill in the art should be understood that; on the basis of technical scheme of the present invention, those skilled in the art do not need to pay various amendment or distortion that creative work can make still within protection scope of the present invention.
Claims (10)
1. one kind utilizes edible fungi residue fermenting and producing cow protein feed new method, it is characterized in that, adopt candida utili bacterium, aspergillus candidus, Lactobacillus plantarum to carry out mixed culture fermentation to edible fungi residue, make the crude protein in bacterium slag, crude fibre changes into the nutriment that can be utilized by milk cow.
2. the method for claim 1, is characterized in that, described edible mushroom is one or more in flat mushroom, pleurotus eryngii, asparagus or white beech mushroom.
3. the method for claim 1, is characterized in that, specifically comprises the steps:
(1) candida utili bacterium, aspergillus candidus, Lactobacillus plantarum are carried out respectively expansion to cultivate, obtain corresponding primary seed solution;
(2) preparation of secondary seed solution: the primary seed solution of candida utili bacterium, aspergillus candidus, Lactobacillus plantarum is inoculated in maize powder medium according to inoculum concentration 1-3%, 1-3%, 2-5%, first aerobic cultivation 18h, then static gas wave refrigerator 24h, is secondary seed solution;
The process of edible fungi residue: get dry mushroom bacterium slag 30-40 part, water 45-55 part, wheat bran 1-5 part, sugared 0.5-1 part, stirs after mixing;
(3) be inoculated in edible fungi residue by the secondary seed solution that step (2) obtains, total inoculum concentration is 5-10%, and at 35 DEG C, quiescent culture 5-7d, to obtain final product.
4. method as claimed in claim 3, it is characterized in that, in step (1), the culture medium of described candida utili, aspergillus candidus is PDA murphy juice fluid nutrient medium.
5. method as claimed in claim 4, it is characterized in that, the preparation method of described PDA murphy juice fluid nutrient medium: potato 200g, add water 1000ml, boiling water boiling half an hour, and filter and add 20g sucrose, 121 DEG C of sterilizing 20min, to obtain final product.
6. method as claimed in claim 3, it is characterized in that, in step (1), the culture medium of described Lactobacillus plantarum is modified MRS culture medium.
7. method as claimed in claim 6, is characterized in that, the preparation method of described modified MRS culture medium: peptone 5g, yeast extract 5g, dipotassium hydrogen phosphate 2.3g, sodium acetate 2g, lactose 2g, glucose 10g, corn flour 10g, epsom salt 0.5g, manganese sulfate 0.2g, water 1000ml, adjustment pH value is 6.2-6.4,121 DEG C of sterilizing 20min, to obtain final product.
8. method as claimed in claim 3, it is characterized in that, in step (2), the preparation method of described maize powder medium: corn flour 20g, brown sugar 10g, lactose 1g, beancake powder 5g, water 1000ml, 121 DEG C of sterilizing 20min, to obtain final product.
9. method as claimed in claim 8, it is characterized in that, 80 mesh sieves crossed by described corn flour.
10. the protein feed prepared of claim 1-6 either method.
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Cited By (6)
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| CN105265769A (en) * | 2015-10-22 | 2016-01-27 | 李德舜 | Multifunctional bacterium fermented compound enzyme feed and preparation method thereof |
| CN106260605A (en) * | 2016-08-15 | 2017-01-04 | 中国农业科学院麻类研究所 | A kind of production method of Hypocrea virens residue fermented feed |
| CN106937704A (en) * | 2017-04-25 | 2017-07-11 | 灌南县畜牧兽医站 | A kind of composite probiotics ferment pleurotus eryngii edible fungi residue feed and its production method |
| CN107535724A (en) * | 2017-09-11 | 2018-01-05 | 广东省农业科学院蚕业与农产品加工研究所 | Needle mushroom pin fermentate and application thereof |
| WO2019046782A3 (en) * | 2017-09-01 | 2019-05-16 | Wild Earth, Inc. | Food product compositions and methods for producing the same |
| US10973242B2 (en) | 2017-09-01 | 2021-04-13 | Wild Earth, Inc. | Protein-containing compositions |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105265769A (en) * | 2015-10-22 | 2016-01-27 | 李德舜 | Multifunctional bacterium fermented compound enzyme feed and preparation method thereof |
| CN105265769B (en) * | 2015-10-22 | 2019-12-10 | 李德舜 | Multifunctional bacterium fermented compound enzyme feed and preparation method thereof |
| CN106260605A (en) * | 2016-08-15 | 2017-01-04 | 中国农业科学院麻类研究所 | A kind of production method of Hypocrea virens residue fermented feed |
| CN106937704A (en) * | 2017-04-25 | 2017-07-11 | 灌南县畜牧兽医站 | A kind of composite probiotics ferment pleurotus eryngii edible fungi residue feed and its production method |
| WO2019046782A3 (en) * | 2017-09-01 | 2019-05-16 | Wild Earth, Inc. | Food product compositions and methods for producing the same |
| US10973242B2 (en) | 2017-09-01 | 2021-04-13 | Wild Earth, Inc. | Protein-containing compositions |
| US11259546B2 (en) | 2017-09-01 | 2022-03-01 | Wild Earth, Inc. | Protein-containing compositions |
| CN107535724A (en) * | 2017-09-11 | 2018-01-05 | 广东省农业科学院蚕业与农产品加工研究所 | Needle mushroom pin fermentate and application thereof |
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