CN105211512A - A kind of fermentable that utilizes is to the method for cotton cake dregs detoxification - Google Patents
A kind of fermentable that utilizes is to the method for cotton cake dregs detoxification Download PDFInfo
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- CN105211512A CN105211512A CN201510791633.3A CN201510791633A CN105211512A CN 105211512 A CN105211512 A CN 105211512A CN 201510791633 A CN201510791633 A CN 201510791633A CN 105211512 A CN105211512 A CN 105211512A
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- cake dregs
- cotton cake
- filter residue
- fermentation
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Abstract
The invention discloses a kind of fermentable that utilizes to the method for cotton cake dregs detoxification, belong to cotton cake dregs field.The present invention first carries out autoclaving with cotton cake dregs, mix with fresh epipremnum aureum juice again, filter, the nutriment such as filter residue and peptone is mixed and made into culture medium, respectively aspergillus niger and yeast strain are cultivated, ferment again after cultivation, filter residue is filtered to obtain after fermentation, by filter residue respectively with dry after acetone and water cleaning, the present invention adopts aspergillus niger and yeast microbes to cultivate, fermentation, free gossypol is combined with gal4 amino acid and forms bound gossoypol, not only reach the object of detoxification, and add protein content and quality in cotton cake dregs, accessory substance can not be produced, and production technology is simple, cost is low.
Description
Technical field
The invention discloses a kind of fermentable that utilizes to the method for cotton cake dregs detoxification, belong to cotton cake dregs field.
Background technology
China is Chan Mian big country main in the world, produces cottonseed more than 800 ten thousand t per year.Wherein cotton benevolence accounts for 50% ~ 55%, and cottonseed kernel protein content is 35 ~ 38%, and the crude protein content carrying the cotton cake dregs after oil can reach 36 ~ 44%.But due to wherein containing the free gossypol to the toxic effect of livestock and poultry, make rich in protein resource fail to be fully used.The utilization rate of current China cotton cake dregs feed is less than 30%.
The poison-removing method of cotton cake dregs is a lot.Wherein, heat treating process is that the free gossypol major part adopting high-temperature and high-pressure conditions to make toxic action strong is converted into nontoxic bound gossoypol; Conventional chemical detoxication method adds sulfate (ferrous sulfate, copper sulphate), and the metal ion in salt and the free gossypol in cotton dregs form complex compound, aldehyde radical and hydroxyl in gossypol are lost activity, reaches detoxification object.Extraction is after employing 2 kinds of solvent mixing, extracts oil and gossypol respectively, to reach the object of removing techniques from cottonseed.Bioanalysis utilizes microbial degradation that gossypol is converted into other innocuous substance, reaches dephenolize object.The research of current this respect is the most active at home.Its detoxification efficiency of different poison-removing methods is widely different, selects suitable dephenolization method to reach better detoxification efficiency, can improve the efficiency of feed utilization of cotton cake dregs.The suitable industrialized preparing process of research removes or free gossypol in passivation cotton cake dregs, makes it the protein feed becoming high-quality, to promoting that the development of feed industry has great showing
Sincere justice.Fermentable detoxicity method is to realize the conversion of gossypol, degradation and detoxification by fermentation process.The successful key of this method selects suitable bacterial classification.Current domestic more existing R&D institutions achieve more significant achievement in the cultivation and screening of virus-free strain.The yeast and mold of screening carries out detoxification test, and the total virus elimination rate of bacterial strain of Highly effected degrading cotton phenol is more than 80%.
Summary of the invention
The technical problem that the present invention mainly solves: contain toxicant free gossypol in current cotton cake dregs, general chemistry, physics dephenolization treating method operability is poor, cost is high, albumen quality after detoxification in cotton cake dregs is poor, accessory substance is many, have a strong impact on the problem of cotton cake dregs application, provide a kind of fermentable that utilizes to the method for cotton cake dregs detoxification, the present invention first carries out autoclaving with cotton cake dregs, mix with fresh epipremnum aureum juice again, filter, the nutriment such as filter residue and peptone is mixed and made into culture medium, respectively aspergillus niger and yeast strain are cultivated, ferment again after cultivation, filter residue is filtered to obtain after fermentation, by filter residue respectively with dry after acetone and water cleaning, the present invention adopts aspergillus niger and yeast microbes to cultivate, fermentation, free gossypol is combined with gal4 amino acid and forms bound gossoypol, not only reach the object of detoxification, and add protein content and quality in cotton cake dregs, accessory substance can not be produced, and production technology is simple, cost is low.
In order to solve the problems of the technologies described above, the technical solution adopted in the present invention is:
(1) get the cotton cake dregs of 500 ~ 600g, added in pulverizer and pulverize, sieve and filter out 50 ~ 60
Object particle, adds water by solid-liquid mass ratio 1:1 and mixes, put it in high-pressure sterilizing pot after stirring in cotton cake dregs powder, boiling 20 ~ 30 DEG C at being arranged on 100 ~ 115 DEG C, controlled pressure, at 0.2 ~ 0.4Mpa, filters after boiling, obtains filter residue solid;
(2) get the fresh epipremnum aureum leaf of 500 ~ 600g, first carried out chopped, then squeezed the juice obtain fresh green
Trailing plants juice, adds above-mentioned cotton cake dregs filter residue and 3 ~ 5g ferrous sulfate heptahydrate, carries out ultrasonic disperse, ultrasonic 10 ~ 15min, obtain even suspension in epipremnum aureum juice;
(3) the above-mentioned suspension of 150 ~ 200mL and 10 ~ 15g peptone is got, 9 ~ 12g beef extract, 15 ~
20g glucose mixes, 2 ~ 2.2L distilled water and 3 ~ 5g Triammonium citrate is added wherein after stirring, continue to be uniformly mixed, the pH of suspension is regulated to be 6 ~ 7 with the oxalic acid solution of mass fraction 15%, suction filtration is carried out after adjustment, the solid obtained by suction filtration is sterilizing 20 ~ 25min at 120 ~ 130 DEG C, can obtain solid medium;
(4) 3 strain Aspergillus niger strains are got and 9 saccharomycete bacterial strains are inoculated in above-mentioned culture medium respectively, be placed in 25 ~
30 DEG C of constant-temperature tables are cultivated respectively, controlled humidity is 50 ~ 60%, aspergillus niger cultivates 16 ~ 24h, 36 ~ 48h cultivated by saccharomycete, is being poured into fermentation tank, and adds the water of 100 ~ 120mL after cultivation in tank, carrying out low temperature, sealed environment bottom fermentation, fermentation temperature is 20 ~ 35 DEG C, and fermentation time is 4 ~ 5 days, filters to obtain filter residue after fermentation;
(5) get 100 ~ 200g filter residue and put into 500mL beaker, add 50 ~ 100mL methanol solution, be placed on magnetic stirrer and stir 25 ~ 30min with the rotating speed of 300 ~ 400r/min, filter afterwards, filter residue is soaked 10 ~ 20min in clear water, moves into centrifugal separator after leaving standstill 5 ~ 8min, carry out centrifugal treating with the rotating speed of 6000 ~ 7000r/min, separated and collected precipitates, and sediment is put into the dry cotton cake dregs that can obtain fermentable detoxification of baking oven of 60 ~ 70 DEG C.
Application process of the present invention: the cotton cake dregs after fermentable detoxification of the present invention is made rich proteinaceous chicken feed, feeding chick, the every day of each interpolation sooner or later once, after 10 ~ 14 days, chick weightening finish raising 15 ~ 17%, in meat, protein content improves 20 ~ 25%, effectively raises the content of protein in chicken.
The invention has the beneficial effects as follows:
(1) the present invention adopts aspergillus niger and yeast microbes to carry out cultivating, fermenting, and free gossypol is combined with gal4 amino acid and forms bound gossoypol, not only reach the object of detoxification, and add protein content and quality in cotton cake dregs;
(2) can not produce accessory substance after utilizing method process cotton cake dregs of the present invention, and production technology is simple, cost is low.
Detailed description of the invention
First get the cotton cake dregs of 500 ~ 600g, added in pulverizer and pulverize, sieve and filter out 50 ~ 60
Object particle, adds water by solid-liquid mass ratio 1:1 and mixes, put it in high-pressure sterilizing pot after stirring in cotton cake dregs powder, boiling 20 ~ 30 DEG C at being arranged on 100 ~ 115 DEG C, controlled pressure, at 0.2 ~ 0.4Mpa, filters after boiling, obtains filter residue solid; Get the fresh epipremnum aureum leaf of 500 ~ 600g, first carried out chopped, then squeezed the juice and obtain fresh epipremnum aureum juice, in epipremnum aureum juice, add above-mentioned cotton cake dregs filter residue and 3 ~ 5g ferrous sulfate heptahydrate, carry out ultrasonic disperse, ultrasonic 10 ~ 15min, obtain even suspension; Get the above-mentioned suspension of 150 ~ 200mL and 10 ~ 15g peptone, 9 ~ 12g beef extract, 15 ~ 20g glucose mixes, 2 ~ 2.2L distilled water and 3 ~ 5g Triammonium citrate is added wherein after stirring, continue to be uniformly mixed, regulate the pH of suspension to be 6 ~ 7 with the oxalic acid solution of mass fraction 15%, after adjustment, carry out suction filtration, the solid obtained by suction filtration is sterilizing 20 ~ 25min at 120 ~ 130 DEG C, can obtain solid medium; Get 3 strain Aspergillus niger strains and 9 saccharomycete bacterial strains are inoculated in above-mentioned culture medium respectively, be placed in 25 ~ 30 DEG C of constant-temperature tables to cultivate respectively, controlled humidity is 50 ~ 60%, and aspergillus niger cultivates 16 ~ 24h, and 36 ~ 48h cultivated by saccharomycete, fermentation tank is being poured into after cultivation, and in tank, add the water of 100 ~ 120mL, carrying out low temperature, sealed environment bottom fermentation, fermentation temperature is 20 ~ 35 DEG C, fermentation time is 4 ~ 5 days, filters to obtain filter residue after fermentation; Get 100 ~ 200g filter residue and put into 500mL beaker, add 50 ~ 100mL methanol solution, be placed on magnetic stirrer and stir 25 ~ 30min with the rotating speed of 300 ~ 400r/min, filter afterwards, filter residue is soaked 10 ~ 20min in clear water, moves into centrifugal separator after leaving standstill 5 ~ 8min, carry out centrifugal treating with the rotating speed of 6000 ~ 7000r/min, separated and collected precipitates, and sediment is put into the dry cotton cake dregs that can obtain fermentable detoxification of baking oven of 60 ~ 70 DEG C.
Example 1
First the cotton cake dregs of 500g is got, added in pulverizer and pulverized, sieve and filter out 50 object particles, add water by solid-liquid mass ratio 1:1 in cotton cake dregs powder and mix, put it into after stirring in high-pressure sterilizing pot, boiling 20 DEG C at being arranged on 100 DEG C, controlled pressure is at 0.2Mpa, filter after boiling, obtain filter residue solid; Get the fresh epipremnum aureum leaf of 500g, first carried out chopped, then squeezed the juice and obtain fresh epipremnum aureum juice, in epipremnum aureum juice, add above-mentioned cotton cake dregs filter residue and 3g ferrous sulfate heptahydrate, carry out ultrasonic disperse, ultrasonic 10min, obtain even suspension; Get the above-mentioned suspension of 150mL and 10g peptone, 9g beef extract, 15g glucose mixes, 2L distilled water and 3g Triammonium citrate is added wherein after stirring, continue to be uniformly mixed, regulate the pH of suspension to be 6 with the oxalic acid solution of mass fraction 15%, after adjustment, carry out suction filtration, the solid obtained by suction filtration is sterilizing 20min at 120 DEG C, can obtain solid medium; Get 3 strain Aspergillus niger strains and 9 saccharomycete bacterial strains are inoculated in above-mentioned culture medium respectively, be placed in 25 DEG C of constant-temperature tables to cultivate respectively, controlled humidity is 50%, and aspergillus niger cultivates 16h, and 36 ~ 48h cultivated by saccharomycete, fermentation tank is being poured into after cultivation, and in tank, add the water of 100mL, carrying out low temperature, sealed environment bottom fermentation, fermentation temperature is 20 DEG C, fermentation time is 4 days, filters to obtain filter residue after fermentation; Get 100g filter residue and put into 500mL beaker, add 50mL methanol solution, be placed on magnetic stirrer and stir 25min with the rotating speed of 300r/min, filter afterwards, filter residue is soaked 10min in clear water, moves into centrifugal separator after leaving standstill 5min, carry out centrifugal treating with the rotating speed of 6000r/min, separated and collected precipitates, and sediment is put into the dry cotton cake dregs that can obtain fermentable detoxification of baking oven of 60 DEG C.
Application process of the present invention: the cotton cake dregs after fermentable detoxification of the present invention is made rich proteinaceous chicken feed, feeding chick, the every day of each interpolation sooner or later once, after 10 days, chick weightening finish raising 15%, in meat, protein content improves 20%, effectively raises the content of protein in chicken.
Example 2
First the cotton cake dregs of 550g is got, added in pulverizer and pulverized, sieve and filter out 55 object particles, add water by solid-liquid mass ratio 1:1 in cotton cake dregs powder and mix, put it into after stirring in high-pressure sterilizing pot, boiling 25 DEG C at being arranged on 108 DEG C, controlled pressure is at 0.3Mpa, filter after boiling, obtain filter residue solid; Get the fresh epipremnum aureum leaf of 550g, first carried out chopped, then squeezed the juice and obtain fresh epipremnum aureum juice, in epipremnum aureum juice, add above-mentioned cotton cake dregs filter residue and 4g ferrous sulfate heptahydrate, carry out ultrasonic disperse, ultrasonic 13min, obtain even suspension; Get the above-mentioned suspension of 170mL and 13g peptone, 10g beef extract, 17g glucose mixes, 2.1L distilled water and 4g Triammonium citrate is added wherein after stirring, continue to be uniformly mixed, regulate the pH of suspension to be 6.5 with the oxalic acid solution of mass fraction 15%, after adjustment, carry out suction filtration, the solid obtained by suction filtration is sterilizing 23min at 125 DEG C, can obtain solid medium; Get 3 strain Aspergillus niger strains and 9 saccharomycete bacterial strains are inoculated in above-mentioned culture medium respectively, be placed in 27 DEG C of constant-temperature tables to cultivate respectively, controlled humidity is 55%, and aspergillus niger cultivates 20h, and 40h cultivated by saccharomycete, fermentation tank is being poured into after cultivation, and in tank, add the water of 110mL, carrying out low temperature, sealed environment bottom fermentation, fermentation temperature is 27 DEG C, fermentation time is 4.5 days, filters to obtain filter residue after fermentation; Get 150g filter residue and put into 500mL beaker, add 75mL methanol solution, be placed on magnetic stirrer and stir 27min with the rotating speed of 350r/min, filter afterwards, filter residue is soaked 15min in clear water, moves into centrifugal separator after leaving standstill 6min, carry out centrifugal treating with the rotating speed of 6500r/min, separated and collected precipitates, and sediment is put into the dry cotton cake dregs that can obtain fermentable detoxification of baking oven of 65 DEG C.
Application process of the present invention: the cotton cake dregs after fermentable detoxification of the present invention is made rich proteinaceous chicken feed, feeding chick, the every day of each interpolation sooner or later once, after 12 days, chick weightening finish raising 16%, in meat, protein content improves 23%, effectively raises the content of protein in chicken.
Example 3
First the cotton cake dregs of 600g is got, added in pulverizer and pulverized, sieve and filter out 60 object particles, add water by solid-liquid mass ratio 1:1 in cotton cake dregs powder and mix, put it into after stirring in high-pressure sterilizing pot, boiling 30 DEG C at being arranged on 115 DEG C, controlled pressure is at 0.4Mpa, filter after boiling, obtain filter residue solid; Get the fresh epipremnum aureum leaf of 600g, first carried out chopped, then squeezed the juice and obtain fresh epipremnum aureum juice, in epipremnum aureum juice, add above-mentioned cotton cake dregs filter residue and 5g ferrous sulfate heptahydrate, carry out ultrasonic disperse, ultrasonic 15min, obtain even suspension; Get the above-mentioned suspension of 200mL and 15g peptone, 12g beef extract, 20g glucose mixes, 2.2L distilled water and 5g Triammonium citrate is added wherein after stirring, continue to be uniformly mixed, regulate the pH of suspension to be 7 with the oxalic acid solution of mass fraction 15%, after adjustment, carry out suction filtration, the solid obtained by suction filtration is sterilizing 25min at 130 DEG C, can obtain solid medium; Get 3 strain Aspergillus niger strains and 9 saccharomycete bacterial strains are inoculated in above-mentioned culture medium respectively, be placed in 30 DEG C of constant-temperature tables to cultivate respectively, controlled humidity is 60%, and aspergillus niger cultivates 24h, and 48h cultivated by saccharomycete, fermentation tank is being poured into after cultivation, and in tank, add the water of 120mL, carrying out low temperature, sealed environment bottom fermentation, fermentation temperature is 35 DEG C, fermentation time is 5 days, filters to obtain filter residue after fermentation; Get 200g filter residue and put into 500mL beaker, add 100mL methanol solution, be placed on magnetic stirrer and stir 30min with the rotating speed of 400r/min, filter afterwards, filter residue is soaked 20min in clear water, moves into centrifugal separator after leaving standstill 8min, carry out centrifugal treating with the rotating speed of 7000r/min, separated and collected precipitates, and sediment is put into the dry cotton cake dregs that can obtain fermentable detoxification of baking oven of 70 DEG C.
Application process of the present invention: the cotton cake dregs after fermentable detoxification of the present invention is made rich proteinaceous chicken feed, feeding chick, the every day of each interpolation sooner or later once, after 14 days, chick weightening finish raising 17%, in meat, protein content improves 25%, effectively raises the content of protein in chicken.
Claims (1)
1. utilize fermentable to a method for cotton cake dregs detoxification, it is characterized in that concrete preparation process is:
(1) get the cotton cake dregs of 500 ~ 600g, added in pulverizer and pulverize, sieve and filter out 50 ~ 60
Order particle, add water by solid-liquid mass ratio 1:1 in cotton cake dregs powder and mix, put it in high-pressure sterilizing pot after stirring, boiling 20 ~ 30 DEG C at being arranged on 100 ~ 115 DEG C, controlled pressure, at 0.2 ~ 0.4Mpa, filters after boiling, obtains filter residue solid;
(2) get the fresh epipremnum aureum leaf of 500 ~ 600g, first carried out chopped, then squeezed the juice obtain fresh green
Trailing plants juice, adds above-mentioned cotton cake dregs filter residue and 3 ~ 5g ferrous sulfate heptahydrate, carries out ultrasonic disperse, ultrasonic 10 ~ 15min, obtain even suspension in epipremnum aureum juice;
(3) the above-mentioned suspension of 150 ~ 200mL and 10 ~ 15g peptone is got, 9 ~ 12g beef extract, 15 ~
20g glucose mixes, 2 ~ 2.2L distilled water and 3 ~ 5g Triammonium citrate is added wherein after stirring, continue to be uniformly mixed, the pH of suspension is regulated to be 6 ~ 7 with the oxalic acid solution of mass fraction 15%, suction filtration is carried out after adjustment, the solid obtained by suction filtration is sterilizing 20 ~ 25min at 120 ~ 130 DEG C, can obtain solid medium;
(4) 3 strain Aspergillus niger strains are got and 9 saccharomycete bacterial strains are inoculated in above-mentioned culture medium respectively, be placed in 25 ~
30 DEG C of constant-temperature tables are cultivated respectively, controlled humidity is 50 ~ 60%, aspergillus niger cultivates 16 ~ 24h, 36 ~ 48h cultivated by saccharomycete, is being poured into fermentation tank, and adds the water of 100 ~ 120mL after cultivation in tank, carrying out low temperature, sealed environment bottom fermentation, fermentation temperature is 20 ~ 35 DEG C, and fermentation time is 4 ~ 5 days, filters to obtain filter residue after fermentation;
(5) get 100 ~ 200g filter residue and put into 500mL beaker, add 50 ~ 100mL methanol solution, be placed on magnetic stirrer and stir 25 ~ 30min with the rotating speed of 300 ~ 400r/min, filter afterwards, filter residue is soaked 10 ~ 20min in clear water, moves into centrifugal separator after leaving standstill 5 ~ 8min, carry out centrifugal treating with the rotating speed of 6000 ~ 7000r/min, separated and collected precipitates, and sediment is put into the dry cotton cake dregs that can obtain fermentable detoxification of baking oven of 60 ~ 70 DEG C.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109170144A (en) * | 2018-11-05 | 2019-01-11 | 扬州市职业大学 | Method based on multi-cultur es cooperative fermentation preparation yellow serofluid and Cottonseed Meal mixed type biology feed |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109170144A (en) * | 2018-11-05 | 2019-01-11 | 扬州市职业大学 | Method based on multi-cultur es cooperative fermentation preparation yellow serofluid and Cottonseed Meal mixed type biology feed |
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Application publication date: 20160106 |