CN105199954A - Shading positioner suitable for DNA sequencing instrument reaction cabin - Google Patents
Shading positioner suitable for DNA sequencing instrument reaction cabin Download PDFInfo
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- CN105199954A CN105199954A CN201510713311.7A CN201510713311A CN105199954A CN 105199954 A CN105199954 A CN 105199954A CN 201510713311 A CN201510713311 A CN 201510713311A CN 105199954 A CN105199954 A CN 105199954A
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- spring
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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Abstract
The invention relates to a shading positioner suitable for a DNA sequencing instrument reaction cabin. The shading positioner comprises a slidable positioning and locking block and a turnover pedestal assembly, and the slidable positioning and locking block and the turnover pedestal assembly are elastically connected and are used for opening and closing a reaction chip mounting seat and a reaction cabin pedestal in a reaction cabin assembly; the slidable positioning and locking block is provided with a locking jaw, and after mounting is completed, the upper end surface of the slidable positioning and locking block is in contact with the inner side surface of the reaction chip mounting seat, and the locking jaw is hooked on the outer side end portion of the reaction chip mounting pedestal; and a shading positioning assembly is fixed to the reaction cabin pedestal, and the slidable positioning and locking block is outward pressed when the reaction chip mounting seat is opened. The shading positioner suitable for the DNA sequencing instrument reaction cabin comprises two symmetrically arranged composition parts, and the reaction cabin assembly can be conveniently opened or closed through a spring assembly between the two parts and a hooking structure, so a chip can be simply and easily replaced, and the shading positioner has the advantages of simple operation and stable structure.
Description
Technical field
The present invention relates to DNA sequencing field, particularly relate to a kind of shading locating device being applicable to DNA sequencer reaction warehouse.
Background technology
In DNA sequencing technical field, reaction chip assembly is as the core of tester, and the accessibility of its operation and the compactedness of structure are important index.
Chinese patent of the prior art " reaction warehouse for DNA sequencer " discloses a kind of reaction warehouse structure, it comprises two parts cooperatively interacted, comprise reaction tank body and mount pad, reaction tank body is provided with annular recesses, mount pad is provided with through installation cavity, installation cavity is provided with multiple locating slot, being provided with in each locating slot slides stretches in installation cavity to hold the slip hyoplastron of sequence testing chip; The first elastomeric element is provided with between reaction tank body and base.
When installing and change reaction chip, because reaction tank body adopts the mode of screw and dead bolt to be connected with the dismounting of mount pad, need unclamp slip hyoplastron and dismantle, can not realize operate continuously, dismounting be inconvenient.
In view of above-mentioned defect, creator of the present invention obtains this creation finally through long research and practice.
Summary of the invention
The object of the present invention is to provide a kind of shading locating device being applicable to DNA sequencer reaction warehouse, in order to overcome above-mentioned technological deficiency.
For achieving the above object, the invention provides a kind of shading locating device being applicable to DNA sequencer reaction warehouse, it comprises slidably positioning and locking block and turnable basement assembly, and both elasticity connect, in order to by the reaction chip mount pad in reaction warehouse assembly and reaction warehouse base open and close;
Described slidably positioning and locking block arranges locking jaw, and after installation is complete, the upper surface of described slidably positioning and locking block contacts with the medial surface of reaction chip mount pad, and locking jaw is hooked on the outboard end of reaction chip mount pad;
Described shading positioning component and reaction warehouse base are fixed together, and when opening reaction chip mount pad, outwards press slidably positioning and locking block, under the action of the spring, slidably positioning and locking block is outward-dipping, and locking jaw and reaction warehouse mount pad depart from;
Described slidably positioning and locking block comprises the elastic locking assembly of pressing plate and the bottom homonymy being arranged on top, wherein,
Described spring is horizontally installed on slidably in positioning and locking block, and described locking jaw is vertically arranged on the end of pressing plate;
Described slidably positioning and locking block and turnable basement assembly are connected, elastic locking assembly comprises symmetrically arranged first spring support, the second spring support, is arranged on the first recess between two spring supports, spring support and the first recess are formed by the isolation of continuous print vertical baffle, and both sides arrange vertical rib;
The centre of described turnable basement is provided with the first projection, and itself and the first recess snap together; First projection both sides are provided with the space that can hold vertical baffle.
Further, in two spring supports, be respectively arranged with spring assembly described, spring assembly comprises spring card slot, lower spring draw-in groove, and upper and lower two spring card slots surround spring eye, and spring eye is shoulder hole, is provided with spring in it;
Wherein, described upper spring card slot is provided with external part, it is arranged hook claw, in order to be connected with the end of described pressing plate.
Further, be provided with the second recess of the spring support described in accommodation in the zygomorphy of the first described projection, the second recess is surrounded by continuous print vertical edge;
Said structure is arranged on integrated second flat board; In use, by spring assembly by being pressed in spring support, slip positioning and locking block and turnable basement assembly are fastened.
Further, be lower convex joint face at the middle portion of described pressing plate, it coordinates with described turnable basement assembly.
Further, described elastic locking assembly is arranged on pressing plate on integrated first flat board, and it is vertical with pressing plate is arranged.
Compared with prior art beneficial effect of the present invention is: the shading locating device being applicable to DNA sequencer reaction warehouse of the present invention, comprise symmetrically arranged two integral parts, by the structure of spring assembly between the two and hook, easily reaction warehouse assembly is opened or close, chip can be changed easily, simple to operate, stabilized structure.
Accompanying drawing explanation
Fig. 1 a is the structural representation of reaction chip assembly of the present invention;
Fig. 1 b is the structural representation of reaction chip mount pad of the present invention;
Fig. 1 c is the structural representation of the medial surface of reaction warehouse base of the present invention;
Fig. 1 d is the structural representation of the outer side of reaction warehouse base of the present invention;
Fig. 1 e is the structural representation of temperature control assembly cover plate of the present invention;
Fig. 2 a is the structural representation of shading positioning component of the present invention;
Fig. 2 b is the first structural representation of slidably positioning and locking block of the present invention;
Fig. 2 c is the second structural representation of slidably positioning and locking block of the present invention;
Fig. 2 d is the structural representation of turnable basement assembly of the present invention;
Fig. 2 e is the structural representation of spring assembly of the present invention;
Fig. 3 a is the sectional structure schematic diagram along liquid inlet and outlet of reaction chip assembly of the present invention;
Fig. 3 b is the sectional structure schematic diagram of turning reaction warehouse assembly of the present invention along self-adaptative adjustment part;
Fig. 3 c is the sectional structure schematic diagram of turning reaction warehouse assembly of the present invention along tail rod;
Fig. 4 a is the structural representation of sealing-ring of the present invention;
Fig. 4 b is sealing-ring sectional structure schematic diagram of the present invention;
Fig. 4 c is the using state schematic diagram that the present invention observes the sealing-ring of right side reaction chip.
Embodiment
Below in conjunction with accompanying drawing, to above-mentioned being described in more detail with other technical characteristic and advantage of the present invention.
Refer to Fig. 1 a to show, the shading positioning component 128 that reaction chip assembly of the present invention comprises reaction chip mount pad 127, reaction warehouse base assembly 126 and reaction warehouse base assembly 126 and reaction chip mount pad 127 locked, opens; In order to take out reaction chip easily and close, reaction chip mount pad 127 can rotate relatively with reaction warehouse base assembly 126.
Refer to Fig. 1 b to show, it is the structural representation of reaction chip mount pad of the present invention, is provided with reaction chip at the middle portion of reaction chip mount pad 127, and reaction chip is provided with cover plate 1277; In the both sides of reaction chip, chute pressing plate 1279 is installed, in order to be fixed reaction chip.A side portion of reaction chip mount pad 127 is provided with mount pad supporting component, its in order to take out or chip placement time, the reaction chip mount pad described in support; It comprises torsional-spring supported portion 1273, be arranged on mount pad, in order to support described torsion spring, described torsional-spring supported portion 1273 is one with the recess of protruding end 1274, when the reaction chip mount pad 127 described in rotating, protruding end 1274 contacts with reaction warehouse base 1261 at first.In the present embodiment, described protruding end 1274 is arranged on the middle part at the edge of recess and chip installation position; Adopt the structure of mount pad itself to support reaction chip mount pad, better reliability, ensure that chip can take out safely and place.
Described mount pad supporting component also comprises torsion spring 1272, be connected with torsion spring 1272 and be arranged on the tension axle 1271 at torsion spring two ends, described tension axle 1271 is arranged on lug boss 1275, when starting reaction chip mount pad, described torsion spring 1272 supports mount pad, and the final location relying on protruding end 1274, realize supporting.
The first rotary shaft hole 1276 is also provided with, in order to be connected with reaction warehouse base 1261 in the end of described lug boss 1275.Locating slot 1278 is also provided with at the sidepiece of reaction chip mount pad.
Refer to Fig. 1 c to show, it is the structural representation of the medial surface of reaction warehouse base of the present invention, reaction tank body 1266 is provided with in the medial surface of reaction warehouse base, at reaction tank body 1266 middle portion, reaction tank 1267 is set, the surrounding 1706 of reaction tank 1267 is provided with sealing-ring, prevents reaction reagent from leaking.One end of reaction warehouse base 1261 is provided with raised line 1705, in order to install after cover plate locate.The other end is provided with the torsional-spring supported structure corresponding with reaction chip mount pad, and it comprises symmetrically arranged two protruding ends 1268, is provided with the second rotary shaft hole 1269 thereon, and it is coaxial with the first rotary shaft hole 1276, and turning axle is successively through holes; Also being provided with the region 1260 for torsion spring activity and locating area 1707 in this end, being provided with in this region in order to the threaded hole 1712 by installing reaction warehouse cover plate.
Refer to Fig. 1 d to show, it is the structural representation of the outer side of reaction warehouse base of the present invention, the middle portion of the outer side of reaction warehouse base is provided with temperature control assembly, the middle portion of reaction warehouse base is provided with temperature sensor mounting groove 1262, the bottom of mounting groove 1262 is provided with nichrome wire mounting groove 1704, places nichrome wire and temperature sensor respectively.In mounting groove, be also provided with a positioning boss 1703, locate when it is in order to install with temperature control assembly cover plate 1263.As shown in fig. le, temperature control assembly cover plate 1263 is provided with the positioning recess 1702 corresponding with positioning boss 1703.
The surrounding of temperature sensor mounting groove 1262 is provided with four tail rod open holess 1742, upper and lower sides is provided with self-adaptation spring mounting hole 1741; Cover plate 1263 is also provided with guiding rod aperture 1701.
Reaction warehouse base is also provided with shading positioning component mounting groove 1265, in order to install described shading positioning component 128, is provided with installation base 1744 within it.
Refer to shown in Fig. 2 a, it is the structural representation of shading positioning component of the present invention, it comprises slidably positioning and locking block 1281 and turnable basement assembly 1283, wherein, described slidably positioning and locking block 1281 is provided with locking jaw 1282, after installation is complete, the upper surface of described slidably positioning and locking block 1281 contacts with the medial surface of reaction chip mount pad 127, and locking jaw 1282 is hooked on the outboard end of reaction chip mount pad 127; Described turnable basement assembly 1283 is arranged on the medial end of reaction warehouse base, is provided with threaded hole 1830.
After installation is complete, described shading positioning component 128 is fixed together with reaction warehouse base, when opening reaction chip mount pad, outside pressing is positioning and locking block 1281 slidably, and under the action of the spring, slidably positioning and locking block 1281 is outward-dipping, locking jaw 1282 and reaction warehouse mount pad depart from, under the effect of the torsion spring of reaction warehouse mount pad end, reaction warehouse mount pad overturns, and can change reaction chip.
In embodiments of the present invention, spring arranged transversely, locking jaw 1282 is vertically arranged, when slidably positioning and locking block described in laterally driving, locking jaw 1282 and reaction chip mount pad 127 depart from, and under the action of the spring, slidably positioning and locking block 1281 slides in the horizontal direction and tilts, so, open slidably positioning and locking block 1281.
Shown in Fig. 2 b, described slidably positioning and locking block 1281 comprises and is arranged on the pressing plate 1823 on top and the elastic locking assembly of bottom homonymy, be lower convex joint face 1821 at the middle portion of pressing plate 1823, it coordinates with described turnable basement assembly 1283.
Shown in Fig. 2 c, described elastic locking assembly comprises symmetrically arranged first spring support 1822, second spring support 1828, be arranged on the first recess 1827 between two spring supports; In two spring supports, be respectively arranged with spring assembly, spring assembly comprises the upper spring card slot 821 shown in figure, lower spring draw-in groove 823, and upper and lower two spring card slots surround spring eye 1771, and spring eye is shoulder hole, is provided with spring 1820 in it; Wherein, upper spring card slot is provided with external part, it is arranged hook claw, in order to be connected with the end of described pressing plate 1823.
In embodiments of the present invention, spring support and the first recess are formed by continuous print vertical baffle 1826 isolation, and both sides arrange vertical rib 1829; Elastic locking assembly is arranged on pressing plate on integrated first flat board 1825, and it is vertical with pressing plate 1823 is arranged.
Refer to shown in Fig. 2 d, the slidably positioning and locking block 1281 in the embodiment of the present invention can symmetrical coordinate with turnable basement assembly 1283, and be provided with the first projection 1832 in the centre of turnable basement, itself and the first recess 1827 snap together; First projection 1832 both sides are provided with the space that can hold vertical baffle 1826; Accordingly, the second recess 1833, second recess of the spring support be provided with described in accommodation in the first projection 1832 zygomorphy is surrounded by continuous print vertical edge 1834; Said structure is arranged on integrated second flat board 1831; In use, by spring assembly by being pressed in spring support, slip positioning and locking block 1281 and turnable basement assembly 1283 are snapped together.
The embodiment of the present invention, arranges shading positioning component, locking reaction chip that not only can be stable, and when changing reaction chip, simple to operate, only need press slip positioning and locking block 1281 and open, and without the need to dismantling reaction chip mount pad, save time.
Refer to shown in Fig. 3 a and 3b, the middle part of reaction warehouse base is provided with electrical heating wire 1754, it is arranged in the nichrome wire mounting groove 1704 of base; In the temperature sensor mounting groove 1262 on electrical heating wire top, be provided with temperature sensor 1720 and control unit, in order to detection reaction pond temperature and according to setting threshold value regulate; Also water at temperature sensor 1720 and control unit 1753 and have sealing member 1263, it is encapsulating resin.
Be respectively arranged with Reagent Tube at the two ends of reaction tank and threaded hole 1721 be installed, in order to be connected with Reagent Tube; The end installing threaded hole 1721 at Reagent Tube is provided with fluid inlet 1723 and liquid outlet 1713, and liquid inlet and outlet communicates with the two ends of reaction tank, in order to cycle detection reagent.
The avris of the interior grooves of reaction tank is provided with sealing-ring 1713.Be provided with reaction chip 04 in the centre of reaction chip mount pad, reagent flows through reaction chip and reacts.
In embodiments of the present invention, Reagent Tube 02 is by the Reagent Tube open holes 1788 of turning warehouse, and the centre being arranged on turning warehouse is provided with the first cavity 1785 holding reagent pipeline, final ligation pond body, reagent cartridge is through the sealing of various cavity, and stability is high, not fragile.
In embodiments of the present invention, in order to ensure that turning reaction warehouse assembly and reaction chip assembly can coaxially movements, convenient adjustment, also be provided with tail rod 1758 between the two, refer to shown in Fig. 3 c, reaction warehouse base arranges guiding rod aperture 1742, turning warehouse arranges tail rod open holes, tail rod 1742 is successively through holes.Described tail rod 1758 comprises reaction warehouse locating flange 1757, in order to locate with guiding rod aperture 1742; Located by nut 1755 end face with tail rod open holes at the other end, between be also provided with pad 1756.
In embodiments of the present invention, in order to test the different positions of reaction chip, adopt the sealing-ring 1713 that can change test area; Refer to shown in Fig. 4 a-4c, described sealing-ring 1713 is hexagon, and it comprises outer rim 1716 and be arranged on the first blend stop 1714 of opposite side and the second blend stop 1715, first blend stop 1714 and the second blend stop 1715 and reagent is divided into three parts.In an embodiment, sidepiece rib and blend stop upper end be respectively first protruding 1718 and the second projection 1719, two projections ranged flow that reagent is being limited, reagent liquid inlet and outlet is arranged in the chamfering between two blend stops.When not using blend stop, it is inner that reagent flows into whole sealing-ring by the chamfering between two blend stops, now, can observe the reaction of whole reaction chip; When state as shown in fig. 4 a, the region of the reaction chip between two blend stops can only be observed; When state as illustrated in fig. 4 c, can only region on the right side of observing response chip.
The embodiment of the present invention adopts foldable sealing-ring, substantially increases the handiness of reaction test, can sample to reaction chip as required.
In embodiments of the present invention, when described reaction warehouse assembly 12 works, above-mentioned reaction chip assembly is arranged in turning reaction warehouse, installs tail rod 1758 and self-adaptative adjustment part 1751, and packaged back shroud; After turnable basement assembly 122 installation, lock turning reaction warehouse and door frame by electronic lock assembly and pressing piece, and the reaction warehouse assembly 12 of installation is arranged on platform assembly 13.
Above-mentioned detailed description is illustrating for one of them possible embodiments of the present invention, and this embodiment is also not used to limit the scope of the claims of the present invention, and the equivalence that all the present invention of disengaging do is implemented or changed, and all should be contained in the scope of technical solution of the present invention.
Claims (5)
1. one kind is applicable to the shading locating device of DNA sequencer reaction warehouse, it is characterized in that, it comprises slidably positioning and locking block and turnable basement assembly, and both elasticity connect, in order to by the reaction chip mount pad in reaction warehouse assembly and reaction warehouse base open and close;
Described slidably positioning and locking block arranges locking jaw, and after installation is complete, the upper surface of described slidably positioning and locking block contacts with the medial surface of reaction chip mount pad, and locking jaw is hooked on the outboard end of reaction chip mount pad;
Described shading positioning component and reaction warehouse base are fixed together, and when opening reaction chip mount pad, outwards press slidably positioning and locking block, under the action of the spring, slidably positioning and locking block is outward-dipping, and locking jaw and reaction warehouse mount pad depart from;
Described slidably positioning and locking block comprises the elastic locking assembly of pressing plate and the bottom homonymy being arranged on top, wherein,
Described spring is horizontally installed on slidably in positioning and locking block, and described locking jaw is vertically arranged on the end of pressing plate;
Described slidably positioning and locking block and turnable basement assembly are connected, elastic locking assembly comprises symmetrically arranged first spring support, the second spring support, is arranged on the first recess between two spring supports, spring support and the first recess are formed by the isolation of continuous print vertical baffle, and both sides arrange vertical rib;
The centre of described turnable basement is provided with the first projection, and itself and the first recess snap together; First projection both sides are provided with the space that can hold vertical baffle.
2. the shading locating device being applicable to DNA sequencer reaction warehouse according to claim 1, it is characterized in that, in two spring supports, spring assembly is respectively arranged with described, spring assembly comprises spring card slot, lower spring draw-in groove, upper and lower two spring card slots surround spring eye, spring eye is shoulder hole, is provided with spring in it;
Wherein, described upper spring card slot is provided with external part, it is arranged hook claw, in order to be connected with the end of described pressing plate.
3. the shading locating device being applicable to DNA sequencer reaction warehouse according to claim 2, is characterized in that, be provided with the second recess of the spring support described in accommodation in the zygomorphy of the first described projection, the second recess is surrounded by continuous print vertical edge;
Said structure is arranged on integrated second flat board; In use, by spring assembly by being pressed in spring support, slip positioning and locking block and turnable basement assembly are fastened.
4. the shading locating device being applicable to DNA sequencer reaction warehouse according to claim 3, is characterized in that, be lower convex joint face at the middle portion of described pressing plate, it coordinates with described turnable basement assembly.
5. the shading locating device being applicable to DNA sequencer reaction warehouse according to claim 3, is characterized in that, described elastic locking assembly is arranged on pressing plate on integrated first flat board, and it is vertical with pressing plate is arranged.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510713311.7A CN105199954B (en) | 2015-10-28 | 2015-10-28 | A kind of shading positioner being applicable to DNA sequencer reaction warehouse |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510713311.7A CN105199954B (en) | 2015-10-28 | 2015-10-28 | A kind of shading positioner being applicable to DNA sequencer reaction warehouse |
Publications (2)
| Publication Number | Publication Date |
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| CN105199954A true CN105199954A (en) | 2015-12-30 |
| CN105199954B CN105199954B (en) | 2016-09-14 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510713311.7A Expired - Fee Related CN105199954B (en) | 2015-10-28 | 2015-10-28 | A kind of shading positioner being applicable to DNA sequencer reaction warehouse |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102703311A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | Reaction bin for adaptive regulation of DNA (Deoxyribose Nucleic Acid) sequencer |
| CN102703312A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | DNA (Deoxyribose Nucleic Acid) sequencer |
| CN102703302A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | Installing seat for automatically clamping sequencing chip |
| CN102766574A (en) * | 2012-05-24 | 2012-11-07 | 中国科学院北京基因组研究所 | Reaction chamber for DNA sequenator |
-
2015
- 2015-10-28 CN CN201510713311.7A patent/CN105199954B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102703311A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | Reaction bin for adaptive regulation of DNA (Deoxyribose Nucleic Acid) sequencer |
| CN102703312A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | DNA (Deoxyribose Nucleic Acid) sequencer |
| CN102703302A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | Installing seat for automatically clamping sequencing chip |
| CN102766574A (en) * | 2012-05-24 | 2012-11-07 | 中国科学院北京基因组研究所 | Reaction chamber for DNA sequenator |
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| Publication number | Publication date |
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| CN105199954B (en) | 2016-09-14 |
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