CN1051938A - The microbiological sensor of rapid determination biological oxygen demand - Google Patents
The microbiological sensor of rapid determination biological oxygen demand Download PDFInfo
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- CN1051938A CN1051938A CN 90106109 CN90106109A CN1051938A CN 1051938 A CN1051938 A CN 1051938A CN 90106109 CN90106109 CN 90106109 CN 90106109 A CN90106109 A CN 90106109A CN 1051938 A CN1051938 A CN 1051938A
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Abstract
The present invention is the microbiological sensor of a rapid determination biological oxygen demand, adopts volt-ampere formula three-electrode system.Wherein working electrode adopts gold (or brass gold filled) disk electrode, and the utmost point is adopted the titanium cylinder electrode, and reference electrode adopts silver/silver chloride electrode.Gold electrode covers microbial film outward, and used bacterial classification can be lichengermium.This sensor electrical electrode potential is stable, and the surface is difficult for passivation, corrosion, and greatly prolong work-ing life.Can be widely used in the environmental monitoring evaluation to water quality.
Description
The invention belongs to the microbiological sensor field, is a kind of microbiological sensor of rapid determination biological oxygen demand.
Biological oxygen demand (Bioc hemical Oxygen Demand, be called for short BOD) is the respiration when being bred by aerobic bacteria, the amount of the oxygen that is consumed when the organism in the water is assimilated.Usually the numerical value of representing with PPM unit through the amount of 5 days time loss oxygen at 20 ℃ is called the BOD value.The BOD value is higher in the water, and expression water quality is contaminated more serious.Therefore, the BOD value is to one of the water quality assessment essential important indicator of measuring in the environmental monitoring.
Classic methods is measured the BOD value and is taken 5 days, can not make correct evaluation at short notice to the pollution level of sewage effluent, therefore also just can't in time control discharging.For this reason, international and domestic all development can rapid determination BOD value method and instrument.Nineteen eighty-two, Suzuki was developed into platinum one plumbous galvanic cell formula BOD microbiological sensor (UP4350763(1982) Monday), its working electrode (anode) is the platinum garden disc electrode of diameter 1cm, to the utmost point (negative electrode) is garden tubular lead electrode, fills 0.1N sodium hydroxide in the battery.Measure the BOD value and only need 10～15 minutes.Because electrode area is bigger, electric current is more stable.Its weak point is the easy passivation of platinum electrode surface.Thereby circulation ratio is relatively poor; Lead electrode corrodes easily, and work-ing life is not long, often needs to change.China Zhang Yusheng etc. levy the volt-ampere formula two electrode system microbiological sensors that the husband proposes according to the light portion of Japan in 1977, having designed with ready-made oxygen measuring instrument is basic instrument, mix the biological response film of immobilized microorganism, make BOD determinator (" environmental science ", 1989 the 10th the 6th phases of volume, P53).The weak point of this design is that diameter is that platinum electrode and large-area microbial film (diameter is 15mm) size about 0.5mm differs greatly, thereby electrode is representative not enough, can not reflect oxygen consumption degree on the whole microbial film.
The object of the present invention is to provide a kind of electrode current good stability, electrode surface is difficult for the burn into purifying, thereby the microbiological sensor of the rapid determination biological oxygen demand of long service life.
BOD microbiological sensor and existing BOD transmitter that the present invention proposes are all inequality, be a kind of be the three-electrode system volt-ampere formula BOD microbiological sensor of working electrode with the big area gold electrode.Wherein gold electrode is coiled in working electrode (negative electrode) employing garden, and diameter is 12～16mm, and thickness is 0.3～0.5mm, and the utmost point (anode) is adopted garden dish type titanium electrode, and being curled by the titanium sheet of length and width in 20～40mm forms.Reference electrode can be with common silver/silver chloride electrode, and the filamentary silver diameter is 0.3～0.7mm, the also available mercurous chloride electrode of reference electrode.Fill 0.05～0.2N Repone K in the electrode.The outer poly tetrafluoroethylene that covers of gold electrode, a topped again immobilized microorganism film, used bacterial classification can be lichengermium, and immobilized method is thalline to be added calcium chloride with sodium alginate make it fixedly film forming.
Accompanying drawing is a structure iron of the present invention, wherein 1 is working electrode, be garden dish gold electrode, 2 is to the utmost point, is garden tubular titanium electrode, 3 is reference electrode, be silver/silver chloride electrode, 4 for covering the poly tetrafluoroethylene outside the gold electrode, and 5 is the immobilized microorganism film, 6 is cellulose acetate film, 7 is synthetic glass working electrode seat, and 8 for filling the electrolytic solution in electrode, generally uses Klorvess Liquid, 9 is the sleeve pipe of working electrode 1 outlet line, adopt nylon material, 10 is the synthetic glass electrode body, and 11 is the synthetic glass turnbuckle, 11 combine with 10 two portions screw threads, 12 is the working electrode outlet line, and 13 is the reference electrode Glass tubing, and 14 is to utmost point outlet line, 15 is unglazed porcelain in the reference electrode, and 16 is the synthetic glass overcoat of electrode.
Working electrode area of the present invention is big, and the even action of microorganism layer is representational. But the oxygen electrode area of volt-ampere formula is big, and electric current is also big, causes potential drop easily and makes the current potential instability, and therefore, the present invention adopts three-electrode system, can obtain stable electrode potential, thereby obtains stable Reduction of oxygen electric current, thereby data are reliable. Because working electrode is gold electrode, compares with platinum electrode, the surface is difficult for passivation. Because the utmost point is adopted large tracts of land titanium electrode, and prolonged use can not corroded, and greatly prolonged electrode life. Reference electrode is because current flowing not, as long as very thin filamentary silver is made silver chloride electrode.
In order to save gold, reduce cost, working electrode of the present invention can be done the basis by brass, outsourcing layer of gold (thickness is generally 0.1-0.2mm).
The three electrode volt-ampere that the present invention the proposes biosensor that declines, can rapid determination water in BOD value, only measured once need 10～15 minutes.But widespread use the evaluation of environmental monitoring to water quality.
Embodiment: with reference to the accompanying drawings, the size of each parts of the present invention is got following numerical value, working electrode 1 adopts gold electrode, and diameter is 14mm, and thickness is 0.5mm, the utmost point 2 is adopted the titanium sheet of 30 * 30mm, be rolled into tubular and become the titanium electrode, reference electrode 3 adopts the Glass tubing of φ 3mm, 0.5mm filamentary silver, gold electrode seat 7 adopts synthetic glass, diameter is 18mm, and electrolytic solution 8 adopts the Repone K (Kcl) of 0.1N, and synthetic glass electrode body 10 diameters are 30mm, highly be 90mm, internal diameter is 20mm, and the inside thread height is 14mm, and synthetic glass thread bush 11 diameters are 20mm, electrode jacket 16 adopts synthetic glass, and diameter is 30mm.Manufacture craft comprises that the preparation technology of microbial film makes identical with common BOD electrode.
Accompanying drawing is BOD microbiological sensor structure chart.
Claims (2)
1, a kind of microbiological sensor of rapid determination biological oxygen demand, adopt volt-ampere formula three-electrode system, reference electrode adopts silver/silver chloride electrode or mercurous chloride electrode, and working electrode is the garden disk shape, and the outside is coated with the immobilized microorganism film, it is characterized in that working electrode adopts gold copper-base alloy, diameter is 12～16mm, and thickness is 0.3～0.5mm, and the utmost point is adopted the titanium material, be shaped as round shape, being curled by the titanium sheet of length in 20-40mm forms.
2, microbiological sensor according to claim 1 is characterized in that its garden dish type working electrode is with brass work basis, the gold of outsourcing one deck 0.1～0.2mm thickness.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 90106109 CN1051938A (en) | 1990-12-29 | 1990-12-29 | The microbiological sensor of rapid determination biological oxygen demand |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 90106109 CN1051938A (en) | 1990-12-29 | 1990-12-29 | The microbiological sensor of rapid determination biological oxygen demand |
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| Publication Number | Publication Date |
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| CN1051938A true CN1051938A (en) | 1991-06-05 |
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| CN 90106109 Pending CN1051938A (en) | 1990-12-29 | 1990-12-29 | The microbiological sensor of rapid determination biological oxygen demand |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101261242B (en) * | 2008-04-21 | 2010-12-22 | 同济大学 | Water quality toxicity biosensor microorganism electrode preparation method |
| CN101206191B (en) * | 2006-12-20 | 2011-12-14 | 株式会社东芝 | Biosensor type exception water quality monitoring apparatus |
| CN102817037A (en) * | 2011-06-10 | 2012-12-12 | 中国石油天然气股份有限公司 | Oil gas pipeline potential measurement permanent reference electrode |
| CN104807864A (en) * | 2015-01-08 | 2015-07-29 | 北京神州瑞霖环保科技有限公司 | Digital electrode for measuring content of perfluorocapylic acid and measuring apparatus |
-
1990
- 1990-12-29 CN CN 90106109 patent/CN1051938A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101206191B (en) * | 2006-12-20 | 2011-12-14 | 株式会社东芝 | Biosensor type exception water quality monitoring apparatus |
| CN101261242B (en) * | 2008-04-21 | 2010-12-22 | 同济大学 | Water quality toxicity biosensor microorganism electrode preparation method |
| CN102817037A (en) * | 2011-06-10 | 2012-12-12 | 中国石油天然气股份有限公司 | Oil gas pipeline potential measurement permanent reference electrode |
| CN102817037B (en) * | 2011-06-10 | 2014-11-26 | 中国石油天然气股份有限公司 | Oil gas pipeline potential measurement permanent reference electrode |
| CN104807864A (en) * | 2015-01-08 | 2015-07-29 | 北京神州瑞霖环保科技有限公司 | Digital electrode for measuring content of perfluorocapylic acid and measuring apparatus |
| CN104807864B (en) * | 2015-01-08 | 2018-07-13 | 西藏神州瑞霖环保科技股份有限公司 | A kind of digital electrode and measurement device measuring perfluoro caprylic acid content |
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