CN105191800A - Virus-free tissue culturing rapid propagation method for trichosanthes - Google Patents
Virus-free tissue culturing rapid propagation method for trichosanthes Download PDFInfo
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- CN105191800A CN105191800A CN201510646356.7A CN201510646356A CN105191800A CN 105191800 A CN105191800 A CN 105191800A CN 201510646356 A CN201510646356 A CN 201510646356A CN 105191800 A CN105191800 A CN 105191800A
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Abstract
The invention provides a virus-free tissue culturing rapid propagation method for trichosanthes and belongs to the technical field of agricultural propagation. The method mainly comprises the following technical steps for operation and treatment: (1) basic culturing: in April, taking the axillary buds of the trichosanthes as explants, cleaning, sterilizing and disinfecting, and treating for 65min at 42 DEG C with hot water, and then passivating the viruses in the materials, and performing basic culturing on the root sections of the trichosanthes; (2) virus-free culturing: taking the axillary buds of the trichosanthes after the basic culturing, performing the virus-free culturing, performing heat treatment on the acquired tissue cultured seedlings for 30 days at 40 DEG C, and then picking off 0.2cm stem tips, and taking the acquired stem tip test tube plantlets as the virus-free seedlings of the trichosanthes; (3) performing multiplying tissue culturing rapid propagation cultivation on the virus-free seedlings of the trichosanthes; and (4) performing rooting culturing by performing rooting propagation on the virus-free seedlings of the trichosanthes after the cultivation. The virus-free tissue culturing rapid propagation method for trichosanthes provided by the invention is fit for industrial seedling breeding; according to the method, the survival rate of the seedlings is greatly increased and the happening of diseases is effectively prevented; and the method is economical and practical.
Description
Technical field
The present invention relates to agriculture raising technology field, particularly relate to a kind of method of snakegourd detoxication and tissue culture rapid propagation.
Background technology
Snakegourd another name balsam pear (Leqing), hang melon (Wenzhou literary composition become), crow melon (Wenzhou).The perennial climbing herb of Curcurbitaceae snake gourd, snakegourd have antipyreticly to quench the thirst, diuresis, the effect such as kobadrin.Seed is containing fat flower fat oil; Fruit is containing triterpenoid saponin, organic acid, resin, carbohydrate, pigment; Root is containing protein, saponin(e, acids.China's most area all has distribution.The method efficiency of tradition seedling fostering is lower, is unfavorable for the popularization of improved seeds, and pole easily infected virus.
Summary of the invention
The object of the present invention is to provide a kind of method of snakegourd detoxication and tissue culture rapid propagation, to solve the problem.
Technical problem to be solved by this invention realizes by the following technical solutions:
A method for snakegourd detoxication and tissue culture rapid propagation, is characterized in that, mainly comprises following operational processes processing step:
(1), basis is cultivated:
April, get the axillalry bud of snakegourd as outside shade, cleaning, sterilizing, after 42 DEG C of hot water treatment 65min, make the virus inactivation in material, above-mentioned snakegourd root segment is carried out basis to cultivate, the formula constituent of basal medium is: MS+6-BA0.7mg/L, NAA0.12mg/L, maltose 15g/L, agar 6g/L;
(2), virus-free culture:
Get the snakegourd axillalry bud after the cultivation of above-mentioned basis, carry out virus-free culture, after the plantlet in vitro obtained heat-treats 30d at 40 DEG C, strip the stem apex of 0.2cm, gained stem apex test-tube plantlet is snakegourd detoxic seedling; The formula constituent of virus-free culture base is MS+6-BA0.7mg/L, NAA0.2mg/L, maltose 15g/L, inositol 80mg/L, burnt ground rice 3g/L, and described burnt ground rice is that under vacuum condition, high temperature makes grain of rice coking, and coking rate is 85%, pulverizes and obtain burnt ground rice;
(3), breed tissue-culturing rapid propagation to cultivate:
Carry out propagation tissue-culturing rapid propagation to snakegourd detoxic seedling to cultivate, the formula constituent of the propagation tissue-culturing rapid propagation medium of snakegourd detoxic seedling is: MS+6-BA1.0mg/L, IBA0.6mg/L, glycine 1.5mg/L, VB1 are 0.8mg/L, VB6 is 0.6mg/L, sweet potato powder 0.2mg/L, inositol 80mg/L, nicotinic acid 0.3mg/L, calcium powder 0.2mg/L;
(4), culture of rootage:
Intensity of illumination is 2000Lx, when environmental temperature is 28 DEG C, under 1/2MS culture medium condition, growth hormone is: IBA1.3mg/L, NAA0.14mg/L, IAA0.3mg/L, proliferation and subculture through 3-5 generation is cultivated, and carries out rooting to the snakegourd group training detoxic seedling after cultivating.
The invention has the beneficial effects as follows:
The method of snakegourd detoxication and tissue culture rapid propagation prepared by the present invention, is applicable to industrialization nursery, greatly improves the survival rate of nursery, and effectively prevent the generation of disease, method is economical and practical.
Embodiment
The technological means realized to make the present invention, creation characteristic, reaching object and effect is easy to understand, below in conjunction with specific embodiment, set forth the present invention further, but following embodiment being only the preferred embodiments of the present invention, and not all.Based on the embodiment in embodiment, those skilled in the art under the prerequisite not making creative work obtain other embodiment, all belong to protection scope of the present invention.
Embodiment 1
A method for snakegourd detoxication and tissue culture rapid propagation, is characterized in that, mainly comprises following operational processes processing step:
(1), basis is cultivated:
April, get the axillalry bud of snakegourd as outside shade, cleaning, sterilizing, after 42 DEG C of hot water treatment 65min, make the virus inactivation in material, above-mentioned snakegourd root segment is carried out basis to cultivate, the formula constituent of basal medium is: MS+6-BA0.7mg/L, NAA0.12mg/L, maltose 15g/L, agar 6g/L;
(2), virus-free culture:
Get the snakegourd axillalry bud after the cultivation of above-mentioned basis, carry out virus-free culture, after the plantlet in vitro obtained heat-treats 30d at 40 DEG C, strip the stem apex of 0.2cm, gained stem apex test-tube plantlet is snakegourd detoxic seedling; The formula constituent of virus-free culture base is MS+6-BA0.7mg/L, NAA0.2mg/L, maltose 15g/L, inositol 80mg/L, burnt ground rice 3g/L, and described burnt ground rice is that under vacuum condition, high temperature makes grain of rice coking, and coking rate is 85%, pulverizes and obtain burnt ground rice;
(3), breed tissue-culturing rapid propagation to cultivate:
Carry out propagation tissue-culturing rapid propagation to snakegourd detoxic seedling to cultivate, the formula constituent of the propagation tissue-culturing rapid propagation medium of snakegourd detoxic seedling is: MS+6-BA1.0mg/L, IBA0.6mg/L, glycine 1.5mg/L, VB1 are 0.8mg/L, VB6 is 0.6mg/L, sweet potato powder 0.2mg/L, inositol 80mg/L, nicotinic acid 0.3mg/L, calcium powder 0.2mg/L;
(4), culture of rootage:
Intensity of illumination is 2000Lx, when environmental temperature is 28 DEG C, under 1/2MS culture medium condition, growth hormone is: IBA1.3mg/L, NAA0.14mg/L, IAA0.3mg/L, proliferation and subculture through 3-5 generation is cultivated, and carries out rooting to the snakegourd group training detoxic seedling after cultivating.
Test material and method:
Test material is excellent snakegourd seedling kind, and have drawn from Agricultural University Of Anhui's snakegourd seedling cultivation base.
Test adopts method for tissue culture to cultivate explant, then adopts and carry out detoxic seedling cultivation to plantlet in vitro stem apex heating means.
| Method | Inoculation number (individual) | Become bud number (individual) | Become bud rate (%) |
| Embodiment 1 | 200 | 122 | 61 |
More than show and describe general principle of the present invention, principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and specification is only preference of the present invention; be not used for limiting the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof.
Claims (1)
1. a method for snakegourd detoxication and tissue culture rapid propagation, is characterized in that, mainly comprises following operational processes processing step:
(1), basis is cultivated:
April, get the axillalry bud of snakegourd as outside shade, cleaning, sterilizing, after 42 DEG C of hot water treatment 65min, make the virus inactivation in material, above-mentioned snakegourd root segment is carried out basis to cultivate, the formula constituent of basal medium is: MS+6-BA0.7mg/L, NAA0.12mg/L, maltose 15g/L, agar 6g/L;
(2), virus-free culture:
Get the snakegourd axillalry bud after the cultivation of above-mentioned basis, carry out virus-free culture, after the plantlet in vitro obtained heat-treats 30d at 40 DEG C, strip the stem apex of 0.2cm, gained stem apex test-tube plantlet is snakegourd detoxic seedling; The formula constituent of virus-free culture base is MS+6-BA0.7mg/L, NAA0.2mg/L, maltose 15g/L, inositol 80mg/L, burnt ground rice 3g/L, and described burnt ground rice is that under vacuum condition, high temperature makes grain of rice coking, and coking rate is 85%, pulverizes and obtain burnt ground rice;
(3), breed tissue-culturing rapid propagation to cultivate:
Carry out propagation tissue-culturing rapid propagation to snakegourd detoxic seedling to cultivate, the formula constituent of the propagation tissue-culturing rapid propagation medium of snakegourd detoxic seedling is: MS+6-BA1.0mg/L, IBA0.6mg/L, glycine 1.5mg/L, VB1 are 0.8mg/L, VB6 is 0.6mg/L, sweet potato powder 0.2mg/L, inositol 80mg/L, nicotinic acid 0.3mg/L, calcium powder 0.2mg/L;
(4), culture of rootage:
Intensity of illumination is 2000Lx, when environmental temperature is 28 DEG C, under 1/2MS culture medium condition, growth hormone is: IBA1.3mg/L, NAA0.14mg/L, IAA0.3mg/L, proliferation and subculture through 3-5 generation is cultivated, and carries out rooting to the snakegourd group training detoxic seedling after cultivating.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105706935A (en) * | 2016-03-14 | 2016-06-29 | 龙岩市禾康生物科技有限公司 | Method for cultivating passion fruit virus-free seedlings |
| CN107155886A (en) * | 2017-05-24 | 2017-09-15 | 四川省苗源生态农业科技有限公司 | A kind of cultural method of virus-free snakegourd |
| CN107593461A (en) * | 2017-11-14 | 2018-01-19 | 李正美 | A kind of tissue culture rapid propagating technology of root of Chinese trichosanthes |
| CN108293878A (en) * | 2018-03-09 | 2018-07-20 | 贵州省生物研究所 | A kind of tissue culture method of snakegourd tender leaf |
| CN108812311A (en) * | 2018-06-06 | 2018-11-16 | 周庆友 | A kind of method of snakegourd quick reproduction technique |
| CN108935099A (en) * | 2018-06-27 | 2018-12-07 | 芜湖东源新农村开发股份有限公司 | The method for tissue culture of Snakegourd Fruit |
| CN108967194A (en) * | 2018-08-07 | 2018-12-11 | 遵义华富生物科技有限公司 | A kind of Chinese medicine melon withers the method for tissue-culturing rapid propagation seedling |
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| CN101699989A (en) * | 2009-11-20 | 2010-05-05 | 杨保成 | Method for tissue culture and rapid propagation of Trichosanthes kirilowii Maxim |
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- 2015-09-30 CN CN201510646356.7A patent/CN105191800A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101699989A (en) * | 2009-11-20 | 2010-05-05 | 杨保成 | Method for tissue culture and rapid propagation of Trichosanthes kirilowii Maxim |
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| 丁茁荑等: "栝蒌脱毒苗组培快繁技术体系研究", 《湖南农业科学》 * |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105706935A (en) * | 2016-03-14 | 2016-06-29 | 龙岩市禾康生物科技有限公司 | Method for cultivating passion fruit virus-free seedlings |
| CN107155886A (en) * | 2017-05-24 | 2017-09-15 | 四川省苗源生态农业科技有限公司 | A kind of cultural method of virus-free snakegourd |
| CN107593461A (en) * | 2017-11-14 | 2018-01-19 | 李正美 | A kind of tissue culture rapid propagating technology of root of Chinese trichosanthes |
| CN108293878A (en) * | 2018-03-09 | 2018-07-20 | 贵州省生物研究所 | A kind of tissue culture method of snakegourd tender leaf |
| CN108812311A (en) * | 2018-06-06 | 2018-11-16 | 周庆友 | A kind of method of snakegourd quick reproduction technique |
| CN108935099A (en) * | 2018-06-27 | 2018-12-07 | 芜湖东源新农村开发股份有限公司 | The method for tissue culture of Snakegourd Fruit |
| CN108967194A (en) * | 2018-08-07 | 2018-12-11 | 遵义华富生物科技有限公司 | A kind of Chinese medicine melon withers the method for tissue-culturing rapid propagation seedling |
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Application publication date: 20151230 |