CN105177061A - Method for increasing acid production of municipal sludge through fermentation by adding external source protease - Google Patents
Method for increasing acid production of municipal sludge through fermentation by adding external source protease Download PDFInfo
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- CN105177061A CN105177061A CN201510613945.5A CN201510613945A CN105177061A CN 105177061 A CN105177061 A CN 105177061A CN 201510613945 A CN201510613945 A CN 201510613945A CN 105177061 A CN105177061 A CN 105177061A
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Abstract
The invention discloses a method for increasing fatty acid generation by municipal sludge through fermentation. The method comprises the steps that thermal alkali pretreatment is conducted on diluted dehydrated residual sludge; the pretreated sludge is transferred into an anaerobic fermentation tank to be subjected to fermentation to generate the fatty acid; when the concentration of the fatty acid in the fermentation tank increases slowly, protease is directly cast into the fermentation tank or supernate is extracted after the sludge in the fermentation tank is centrifuged, centrifugation is conducted again after the pH of the supernate is regulated until the supernate is acid, sediment is recycled and dissolved with water, then the protease is added into the sediment, and anaerobic fermentation is conducted continually to produce the fatty acid.
Description
Technical field
The invention belongs to field of solid waste resource utilization.More specifically, the present invention relates to a kind of method improving municipal sludge fermentation and acid by adding exogenous protease.
Background technology
Sludge fermentation produce acid for realizing reducing urban sludge, recycling provides a new thinking.Cheapness organic matter in mud is produced and is converted into high added value biochemical products, reduce excess sludge production; And, change the organism of anaerobic digester system into organic acid, instead of GHG carbon dioxide and methane, have great significance to realizing carbon emission reduction.In current alkaline fermentation process, dissolved organic matters still residual a large amount of in the slag of natural pond, as: the protein accounting for total SCOD about 30%; The carbohydrate of 10%, nutritive substance itself can bioavailability may be the major cause causing alkaline fermentation productive rate still lower.Sludge pretreatment is the effective technology improving mud biodegradability.As usually adopted ultrasonic, the pretreatment process such as hot acid, thermokalite, enzyme process.After ultrasonic-alkaline purification, the stripping of SCOD can improve 52%, and after thermokalite and ultrasonic-oxygenation pretreatment, the stripping of VS is more than 60%, and after sludge hot hydrolysis, voltaile fatty acid (VFAs) output can improve 2-5 doubly.Heat or ultraviolet pre-treatment can improve the utilization ratio 30%-40% of protein.But early stage, the research about sludge pretreatment was for before sludge fermentation, broken wall, the dissolving of raising nutrition etc., make organism fully discharge dissolving, can by the easier contact of acid-producing microorganisms and utilization.Utilize enzyme process pretreating sludge to improve in mud biodegradability, the organism amount of stripping is few, and the transformation efficiency of substrate is close with utilizing the transformation efficiency of other pretreatment processs.And for the protein that difficult for biological degradation residual after fermentation utilizes, the organism such as carbohydrate are hydrolyzed further, utilize and also there is no related art method.
The heavy metal ion stabilization that the people such as Sun Xiangzhang (CN102010109A) utilize biological enzyme to make in mud, and the macromole in mud is changed into small molecules.The present invention utilizes proteolytic enzyme to improve sludge fermentation to produce the output of acid and the transformation efficiency of substrate.The people such as Luo Kun (CN102211842A) utilize prozyme to carry out pre-treatment to municipal sludge, and object is the organism in stripping mud.The present invention is then the solvability protein thus the further output improving voltaile fatty acid that remain after utilizing proteolytic enzyme to transform sludge anaerobic fermentation.It is high that this method for sludge treatment has organism utilization rate, the features such as production of organic acids is high, simple to operation; For finally realizing the resource utilization of excess sludge, minimizing and innoxious, provide a kind of new method and thinking.
Summary of the invention
The object of the invention is to provide a kind of method improving municipal sludge fermentation and acid by adding exogenous protease, concrete method content is: after city dehydrated sludge is diluted to finite concentration, regulate pH to 10-12, more than 70 DEG C, stir more than pre-treatment 1h subsequently.Transferred in anaerobic fermentation tank by pretreated mud subsequently and carry out fermentation and acid, the pH maintaining fermentation system is 8-11.Protein, polysaccharide, lipid acid in every day monitoring reactor.When the fatty acid concentration in reactor increasess slowly, directly to adding in reactor >=proteolytic enzyme of 10mg/L, such as Sumizyme MP or trypsinase.Or after mud centrifugal segregation, in supernatant liquor, add hydrochloric acid, by pH regulator to recentrifuge after acid, reclaim throw out, be the residual organic matter of extraction, then add by water dissolution >=proteolytic enzyme of 10mg/L proceeds anaerobically fermenting and produces lipid acid.
More specifically, the invention provides the following:
1. improve the method that lipid acid is produced in municipal sludge fermentation, described method comprises: carry out alkaline Pretreatment to excess sludge; Pretreated mud is transferred in anaerobic fermentation tank and carry out fermentation product lipid acid; And, when the fatty acid concentration in described fermentor tank increasess slowly, proteolytic enzyme is added directly to described fermentor tank, or after centrifugal for the mud in described fermentor tank, get supernatant liquor, by the pH regulator of described supernatant liquor to acid rear recentrifuge, reclaim throw out, described throw out water dissolution is also added wherein proteolytic enzyme and proceed anaerobically fermenting product lipid acid.
2. the method for the 1st, wherein carries out described alkaline Pretreatment: as follows by the city dehydrated sludge pH regulator of dilution to 10-12, more than stir process 1h more than 70 DEG C subsequently.
3. the method for the 2nd, wherein said alkaline Pretreatment pH10-12,90 DEG C carry out.
4. the method for the 1st, wherein said proteolytic enzyme is Sumizyme MP.
5. the method for the 1st, wherein said proteolytic enzyme is trypsinase.
6. the method for the 1st, wherein adds described proteolytic enzyme with the concentration of >=10mg/L (such as 20mg/L).
7. the method for the 1st, wherein produces in lipid acid process in described fermentation and maintains that pH is 8-11, temperature is 37 DEG C.
8. the method for the 1st, wherein in described fermentation product lipid acid process, maintaining pH is 10.
9. the method for the 1st, the pH of wherein said supernatant liquor is adjusted to 3.3.
10. the method for the 1st, the excess sludge that wherein will carry out described alkaline Pretreatment is the dehydration excess sludge of dilution, and the concentration of the dehydration excess sludge of described dilution is 75g/L.
Accompanying drawing explanation
Fig. 1 is pretreating sludge alkaline fermentation substrate situation map.
Fig. 2 is that the organism such as protein extracted adds protein and fermenting substrate acid yield changing trend diagram after enzyme.
Fig. 3 be add lytic enzyme after produce sour situation map and degradation of substrates figure.
Embodiment
Below by specific embodiment, the present invention will be further described, but the present invention is not limited thereto.
Experimental technique described in following embodiment, if no special instructions, is ordinary method; Described reagent and material, if no special instructions, all can obtain from commercial channels.
" excess sludge (excessactivatedsludge) " that use in the present invention comes from Sludge System from the active sludge secondary sedimentation basins (or settling region) discharge system.After alkaline Pretreatment, mud organic substance composition and content are shown in Fig. 1.
Embodiment 1
Regulate pH to 12 with hydrochloric acid or sodium hydroxide/calcium hydroxide after the concentration of the excess sludge that dewaters (from Wuxi new city sewage work) being diluted with water to 75g/L, stir pre-treatment 2h at 90 DEG C subsequently.Subsequently the mud after process is added 3L anaerobic fermentation tank (Pr-, Zhenjiang Oriental Bio-engineering Technology Co., Ltd), regulating in fermenting process and maintaining pH is 10, temperature is 37 DEG C, protein in every day monitoring reactor, polysaccharide, the concentration of lipid acid, (ferment after 7 days when the concentration of VFA increasess slowly, the concentration of VFA maintains 11895mg/L) (Fig. 2), carry out three groups of experiments, respectively: in fermentation system, add 20mg/L Sumizyme MP (10millionU/g, AmanoEnzymeManufacturingCo., Ltd., and adjust pH=10 Japan), 20mg/L trypsin 5millionU/g is added, AmanoEnzymeManufacturingCo., Ltd., Japan in fermentation system) and adjust pH=8, and, blank assay (not adding proteolytic enzyme, adjustment pH=10).Continue fermentation and acid 6 days, the organic acid concentration of first two groups is respectively 18540 and 13500mg/L.This sample result shows, and the protein transform rate that interpolation Sumizyme MP and trypsinase make sludge anaerobic ferment respectively improves 44.4% and 75% than control group, and in sludge hydrolytic liquid, VFA output improves 55.7% and 133.7% respectively.
Embodiment 2
Regulate pH to 12 with hydrochloric acid or sodium hydroxide/calcium hydroxide after the concentration of the excess sludge that dewaters (from Wuxi new city sewage work) being diluted with water to 75g/L, stir pre-treatment 2h at 90 DEG C subsequently.Subsequently the mud after process is added 3L anaerobic fermentation tank (Pr-, Zhenjiang Oriental Bio-engineering Technology Co., Ltd), regulate in fermenting process and maintain pH10, temperature 37 DEG C.Protein in every day monitoring reactor, polysaccharide, the concentration of lipid acid, (ferment after 7 days when organic acid concentration increasess slowly, organic acid concentration rises to 11895mg/L), the centrifugal fermented sludge 30min of 1000 × g, get supernatant liquor and with hydrochloric acid or sodium hydroxide/calcium hydroxide by its pH regulator to 3.3, stir 1000Xg after 30 minutes centrifugal, get precipitation water dissolution and add 20mg/L Sumizyme MP (10millionU/g wherein, AmanoEnzymeManufacturingCo., Ltd., and adjust pH=10 Japan), or add trypsin 5millionU/g, AmanoEnzymeManufacturingCo., Ltd., and adjust pH=8 Japan), or do not having in the organism situation needed for fermentation, adjustment pH=10 (as blank assay), proceed fermentation and acid 5-6 days, VFAs concentration is respectively 8000 and 7292, 1000mg/L.As shown in Figure 3, after implementing this method, protein transform rate has brought up to 77% and 79.9% respectively, and in sludge hydrolytic liquid, VFA output improves 67.3% and 61.3% respectively.
Although the present invention with preferred embodiment openly as above; but it is also not used to limit the present invention, any person skilled in the art, without departing from the spirit and scope of the present invention; all can do various changes and modification, what therefore protection scope of the present invention should define with claims is as the criterion.
Claims (10)
1. improve the method that lipid acid is produced in municipal sludge fermentation, described method comprises: carry out alkaline Pretreatment to excess sludge; Pretreated mud is transferred in anaerobic fermentation tank and carry out fermentation product lipid acid; And, when the fatty acid concentration in described fermentor tank increasess slowly, proteolytic enzyme is added directly to described fermentor tank, or after centrifugal for the mud in described fermentor tank, get supernatant liquor, by the pH regulator of described supernatant liquor to acid rear recentrifuge, reclaim throw out, described throw out water dissolution is also added wherein proteolytic enzyme and proceed anaerobically fermenting product lipid acid.
2. the process of claim 1 wherein and carry out described alkaline Pretreatment as follows: by the city dehydrated sludge pH regulator of dilution to 10-12, more than stir process 1h more than 70 DEG C subsequently.
3. the method for claim 2, wherein said alkaline Pretreatment pH10-12,90 DEG C carry out.
4. the process of claim 1 wherein that described proteolytic enzyme is Sumizyme MP.
5. the process of claim 1 wherein that described proteolytic enzyme is trypsinase.
6. the process of claim 1 wherein and add described proteolytic enzyme with the concentration of >=10mg/L (such as 20mg/L).
7. the process of claim 1 wherein to produce in lipid acid process in described fermentation and maintain that pH is 8-11, temperature is 37 DEG C.
8. the process of claim 1 wherein that in described fermentation product lipid acid process, maintain pH is 10.
9. the process of claim 1 wherein that the pH of described supernatant liquor is adjusted to 3.3.
10. the process of claim 1 wherein that the excess sludge that will carry out described alkaline Pretreatment is the dehydration excess sludge of dilution, the concentration of the dehydration excess sludge of described dilution is 75g/L.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109336348A (en) * | 2018-11-16 | 2019-02-15 | 江南大学 | A kind of kitchen garbage strengthens aspergillus oryzae and lays eggs white enzyme and the method that promotes excess sludge anaerobism digest production biogas altogether |
| CN109355215A (en) * | 2018-11-28 | 2019-02-19 | 同济大学 | Dewatered sludge prepares the method and its application of oleaginous yeast fermentation medium |
| CN112280768A (en) * | 2020-10-26 | 2021-01-29 | 林小丽 | Alkaline protease low-temperature mutant and application thereof in sludge treatment |
| CN112458070A (en) * | 2020-10-26 | 2021-03-09 | 林小丽 | Enzyme preparation for sludge treatment |
| CN114410700A (en) * | 2022-02-15 | 2022-04-29 | 苏州美淼环保科技有限公司 | Sludge hydrolysate secondary anaerobic fermentation acid production stimulation method for avoiding and promoting combination |
| CN114958812A (en) * | 2022-06-27 | 2022-08-30 | 上海交通大学 | Method for promoting sludge feed conversion by using complex enzyme |
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| CN104862346A (en) * | 2015-05-04 | 2015-08-26 | 同济大学 | Method for increasing yield of short chain fatty acid produced with excess sludge through enzyme and alkali combined pretreatment |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104862346A (en) * | 2015-05-04 | 2015-08-26 | 同济大学 | Method for increasing yield of short chain fatty acid produced with excess sludge through enzyme and alkali combined pretreatment |
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| FLORIAN MONLAU等: "Two-Stage Alkaline−Enzymatic Pretreatments To Enhance Biohydrogen Production from Sunflower Stalks", 《ENVIRON. SCI. TECHNOL.》 * |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109336348A (en) * | 2018-11-16 | 2019-02-15 | 江南大学 | A kind of kitchen garbage strengthens aspergillus oryzae and lays eggs white enzyme and the method that promotes excess sludge anaerobism digest production biogas altogether |
| CN109355215A (en) * | 2018-11-28 | 2019-02-19 | 同济大学 | Dewatered sludge prepares the method and its application of oleaginous yeast fermentation medium |
| CN112280768A (en) * | 2020-10-26 | 2021-01-29 | 林小丽 | Alkaline protease low-temperature mutant and application thereof in sludge treatment |
| CN112458070A (en) * | 2020-10-26 | 2021-03-09 | 林小丽 | Enzyme preparation for sludge treatment |
| CN114410700A (en) * | 2022-02-15 | 2022-04-29 | 苏州美淼环保科技有限公司 | Sludge hydrolysate secondary anaerobic fermentation acid production stimulation method for avoiding and promoting combination |
| CN114958812A (en) * | 2022-06-27 | 2022-08-30 | 上海交通大学 | Method for promoting sludge feed conversion by using complex enzyme |
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Application publication date: 20151223 |