CN105177057A - Method for regulating pH of high-concentration substrate in pretreatment and enzymolysis process - Google Patents
Method for regulating pH of high-concentration substrate in pretreatment and enzymolysis process Download PDFInfo
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- CN105177057A CN105177057A CN201510734430.0A CN201510734430A CN105177057A CN 105177057 A CN105177057 A CN 105177057A CN 201510734430 A CN201510734430 A CN 201510734430A CN 105177057 A CN105177057 A CN 105177057A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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Abstract
The invention relates to a method for regulating pH of a high-concentration substrate in a pretreatment and enzymolysis process, which comprises the following steps: (1) sampling the high-concentration substrate to be subjected to pH regulation, adding water for dilution, regulating the diluted sample to the target pH, and recording the volume v of the reagent used by regulating the diluted sample to the target pH; substituting in the formula disclosed in the specification, and calculating the volume V of the reagent used by regulating the high-concentration substrate to be subjected to pH regulation to the target pH; adding the reagent with the corresponding amount capable of regulating the high-concentration substrate to be subjected to pH regulation to the target pH into the high-concentration substrate to be subjected to pH regulation, and sufficiently and uniformly mixing, thereby completing the pH regulation of the high-concentration substrate. The acid/alkali amount required by the known small amount of diluted high-concentration substrate is utilized to calculate the acid/alkali amount required by the total sample; and the required amount of acid/alkali and the high-concentration substrate to be regulated can be directly mixed and sufficiently stirred, so that the whole system is uniform.
Description
Technical field
The present invention relates to a kind of method regulating high concentration substrate pH in pre-treatment, enzymolysis process.
Background technology
For making s-generation alcohol fuel, there is Economic competitive, the ethanol obtaining high density (>5%) is a precondition, reach high quality, the ethanol of high density carries out high concentration substrate process with regard to needs in pre-treatment and enzymic hydrolysis stage, in whole operating process, pH needs to regulate in different steps.
But liquid or low-concentration fluid are easy to carry out acid-alkali accommodation.But the system of high density is then difficult to.Especially the lignocellulose of more than 25% (w/v), water-absorbent is very strong, almost not free liquid, mass transfer is very difficult, cause pH regulator to be difficult to carry out under the dense condition of height, and consider from Financial cost, it is a lot of again that the lignocellulose of high density operates in field of biological energy source, how to regulate the pH of high dense substrate to be a key issue, the adjustment of pH is related to the success or failure of pre-treatment and enzymolysis.
Summary of the invention
For the deficiencies in the prior art, the invention provides a kind of method regulating high concentration substrate pH in pre-treatment, enzymolysis process.
Technical scheme of the present invention is as follows:
Regulate a method of high concentration substrate pH in pre-treatment, enzymolysis process, step is as follows:
(1) the high concentration substrate sampling of adjusting pH will be waited, and record sampling amount m, the sample thin up of taking-up, being diluted to mass concentration is 5 ~ 15wt%, sample after dilution is adjusted to target pH, and the sample after record dilution is adjusted to the volume v of target pH agents useful for same;
(2) survey the total mass waiting the high concentration substrate adjusting pH, by the volume of sampling amount m, target pH agents useful for same in step (1), wait that the total mass number of the high concentration substrate adjusting pH is according to substitution formula:
calculate and wait to adjust the high concentration substrate of pH to be adjusted to the volume V of target pH agents useful for same;
In formula, W is the total mass waiting the high concentration substrate adjusting pH, unit: g
V waits to adjust the high concentration substrate of pH to be adjusted to the volume of target pH agents useful for same, volume unit, ml
Sample after v=dilution is adjusted to the volume of target pH agents useful for same, volume unit, ml
M is sampling amount, unit: g
(3) what step (2) calculated waits that the amount adjusting the high concentration substrate of pH to be adjusted to target pH agents useful for same adds in the high concentration substrate waiting to adjust pH, is fully uniformly mixed, namely completes the adjustment of high concentration substrate pH.
According to the present invention, preferably, in step (1), sampling amount m is 5 ~ 10g.
According to the present invention, preferably, the substrate mass concentration in step (1) after dilution is 5 ~ 10wt%.Prove there is free fluid in this concentration range through numerous experiments, regulate pH to be easier to.
According to the present invention, preferably, step (1) middle and high concentration substrate mass concentration is 25 ~ 40wt%.
According to the present invention, preferably, being adjusted to target pH agents useful for same is sodium hydroxide, sodium bicarbonate, hydrochloric acid or sulfuric acid.
The present invention is preferred, and described h 2 so 4 concentration is 70 ~ 98wt%.
Preferred according to the present invention, be that 11 ~ 12 to be transferred to pH be 5 ~ 5.5 by high concentration substrate from pH, agents useful for same is the sulfuric acid of 72%.
If need total wooden cellulose amount to be processed greatly can with the vitriol oil of 98%.PH5 ~ 5.5 are the Optimal pH of cellulase and hemicellulase hydrolyzes.
According to the present invention, preferably, in order to make pH regulator full and uniform, step (3), is uniformly mixed laggard row and carries out standing 4 ~ 7h.
Beneficial effect of the present invention is as follows:
Soda acid amount needed for after the present invention utilizes the dense substrate of the height of known a small amount of to dilute is to calculate the soda acid amount required for total sample.Then directly the dense substrate of height that required soda acid and needs regulate directly can be mixed, fully stir and mix completely, make whole system homogeneous.PH regulator can be made like this to arrive object value.The feasible Another reason of this law starts to only have part substrate hydrolysis, but along with the release of liquid, solids concn reduces gradually, and mass transfer problem is more and more less.
Embodiment
Below by specific embodiment, the present invention will be further described, but be not limited thereto.
Embodiment 1
Regulate a method of high concentration substrate pH in pre-treatment, enzymolysis process, step is as follows:
(1) will wait the high concentration substrate sampling of adjusting pH, high concentration substrate mass concentration is 25 ~ 40wt%.And record sampling amount m, sampling amount m is 5g, the sample thin up taken out, being diluted to mass concentration is 6wt%, sample after dilution is adjusted to target pH, be that 11.2 to be transferred to pH be 5.2 by high concentration substrate from pH, agents useful for same is the sulfuric acid of 72%, and the sample after record dilution is adjusted to the volume v of target pH agents useful for same (sulfuric acid of 72%);
(2) survey the total mass waiting the high concentration substrate adjusting pH, by the volume of sampling amount m, target pH agents useful for same in step (1), wait that the total mass number of the high concentration substrate adjusting pH is according to substitution formula:
calculate and wait to adjust the high concentration substrate of pH to be adjusted to the volume V of target pH agents useful for same;
In formula, W is the total mass waiting the high concentration substrate adjusting pH, unit: g
V waits to adjust the high concentration substrate of pH to be adjusted to the volume of target pH agents useful for same, volume unit, ml
Sample after v=dilution is adjusted to the volume of target pH agents useful for same, volume unit, ml
M is sampling amount, unit: g
(3) what step (2) calculated waits that the amount adjusting the high concentration substrate of pH to be adjusted to target pH agents useful for same adds in the high concentration substrate waiting to adjust pH, is fully uniformly mixed, leaves standstill 6h, namely complete the adjustment of high concentration substrate pH.
Embodiment 2
Regulate a method of high concentration substrate pH in pre-treatment, enzymolysis process, step is as follows:
(1) will wait the high concentration substrate sampling of adjusting pH, high concentration substrate mass concentration is 25 ~ 40wt%.And record sampling amount m, sampling amount m is 10g, the sample thin up taken out, being diluted to mass concentration is 8wt%, sample after dilution is adjusted to target pH, be that 11.2 to be transferred to pH be 5.2 by high concentration substrate from pH, agents useful for same is the sulfuric acid of 72%, and the sample after record dilution is adjusted to the volume v of target pH agents useful for same (sulfuric acid of 72%);
(2) survey the total mass waiting the high concentration substrate adjusting pH, by the volume of sampling amount m, target pH agents useful for same in step (1), wait that the total mass number of the high concentration substrate adjusting pH is according to substitution formula:
calculate and wait to adjust the high concentration substrate of pH to be adjusted to the volume V of target pH agents useful for same;
In formula, W is the total mass waiting the high concentration substrate adjusting pH, unit: g
V waits to adjust the high concentration substrate of pH to be adjusted to the volume of target pH agents useful for same, volume unit, ml
Sample after v=dilution is adjusted to the volume of target pH agents useful for same, volume unit, ml
M is sampling amount, unit: g
(3) what step (2) calculated waits that the amount adjusting the high concentration substrate of pH to be adjusted to target pH agents useful for same adds in the high concentration substrate waiting to adjust pH, is fully uniformly mixed, leaves standstill 7h, namely complete the adjustment of high concentration substrate pH.
Regulate a method of high concentration substrate pH in pre-treatment, enzymolysis process, step is as follows:
(1) the high concentration substrate sampling of adjusting pH will be waited, and record sampling amount m, the sample thin up of taking-up, being diluted to mass concentration is 5 ~ 15wt%, sample after dilution is adjusted to target pH, and the sample after record dilution is adjusted to the volume v of target pH agents useful for same;
(2) survey the total mass waiting the high concentration substrate adjusting pH, by the volume of sampling amount m, target pH agents useful for same in step (1), wait that the total mass number of the high concentration substrate adjusting pH is according to substitution formula:
calculate and wait to adjust the high concentration substrate of pH to be adjusted to the volume V of target pH agents useful for same;
In formula, W is the total mass waiting the high concentration substrate adjusting pH, unit: g
V waits to adjust the high concentration substrate of pH to be adjusted to the volume of target pH agents useful for same, volume unit, ml
Sample after v=dilution is adjusted to the volume of target pH agents useful for same, volume unit, ml
M is sampling amount, unit: g
(3) what step (2) calculated waits that the amount adjusting the high concentration substrate of pH to be adjusted to target pH agents useful for same adds in the high concentration substrate waiting to adjust pH, is fully uniformly mixed, namely completes the adjustment of high concentration substrate pH.
Claims (8)
1. regulate a method of high concentration substrate pH in pre-treatment, enzymolysis process, step is as follows:
(1) the high concentration substrate sampling of adjusting pH will be waited, and record sampling amount m, the sample thin up of taking-up, being diluted to mass concentration is 5 ~ 15wt%, sample after dilution is adjusted to target pH, and the sample after record dilution is adjusted to the volume v of target pH agents useful for same;
(2) survey the total mass waiting the high concentration substrate adjusting pH, by the volume of sampling amount m, target pH agents useful for same in step (1), wait that the total mass number of the high concentration substrate adjusting pH is according to substitution formula:
calculate and wait to adjust the high concentration substrate of pH to be adjusted to the volume V of target pH agents useful for same;
In formula, W is the total mass waiting the high concentration substrate adjusting pH, unit: g
V waits to adjust the high concentration substrate of pH to be adjusted to the volume of target pH agents useful for same, volume unit, ml
Sample after v=dilution is adjusted to the volume of target pH agents useful for same, volume unit, ml
M is sampling amount, unit: g
(3) what step (2) calculated waits that the amount adjusting the high concentration substrate of pH to be adjusted to target pH agents useful for same adds in the high concentration substrate waiting to adjust pH, is fully uniformly mixed, namely completes the adjustment of high concentration substrate pH.
2. according to the method regulating high concentration substrate pH in the pre-treatment described in claim 1, enzymolysis process, it is characterized in that, in step (1), sampling amount m is 5 ~ 10g.
3. according to the method regulating high concentration substrate pH in the pre-treatment described in claim 1, enzymolysis process, it is characterized in that, the substrate mass concentration in step (1) after dilution is 5 ~ 10wt%.
4. according to the method regulating high concentration substrate pH in the pre-treatment described in claim 1, enzymolysis process, it is characterized in that, step (1) middle and high concentration substrate mass concentration is 25 ~ 40wt%.
5. according to the method regulating high concentration substrate pH in the pre-treatment described in claim 1, enzymolysis process, it is characterized in that, being adjusted to target pH agents useful for same is sodium hydroxide, sodium bicarbonate, hydrochloric acid or sulfuric acid.
6. according to the method regulating high concentration substrate pH in the pre-treatment described in claim 5, enzymolysis process, it is characterized in that, described h 2 so 4 concentration is 70 ~ 98wt%.
7. according to the method regulating high concentration substrate pH in the pre-treatment described in claim 6, enzymolysis process, it is characterized in that, be that 11 ~ 12 to be transferred to pH be 5 ~ 5.5 by high concentration substrate from pH, agents useful for same is the sulfuric acid of 72%.
8. according to the method regulating high concentration substrate pH in the pre-treatment described in claim 1, enzymolysis process, it is characterized in that, in order to make pH regulator full and uniform, step (3), is uniformly mixed laggard row and carries out standing 4 ~ 7h.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510734430.0A CN105177057A (en) | 2015-11-02 | 2015-11-02 | Method for regulating pH of high-concentration substrate in pretreatment and enzymolysis process |
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| CN201510734430.0A CN105177057A (en) | 2015-11-02 | 2015-11-02 | Method for regulating pH of high-concentration substrate in pretreatment and enzymolysis process |
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| CN105177057A true CN105177057A (en) | 2015-12-23 |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1328275A (en) * | 1999-12-15 | 2001-12-26 | 康景轩 | Method and device for automatically regulating pH valve |
| CN104651429A (en) * | 2015-02-03 | 2015-05-27 | 湖南农业大学 | Method for realizing high substrate concentration enzyme hydrolysis of lignocellulose by irradiation pretreatment and batch feeding |
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- 2015-11-02 CN CN201510734430.0A patent/CN105177057A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1328275A (en) * | 1999-12-15 | 2001-12-26 | 康景轩 | Method and device for automatically regulating pH valve |
| CN104651429A (en) * | 2015-02-03 | 2015-05-27 | 湖南农业大学 | Method for realizing high substrate concentration enzyme hydrolysis of lignocellulose by irradiation pretreatment and batch feeding |
Non-Patent Citations (1)
| Title |
|---|
| 佟昀等: "《机织试验与设备实训》", 31 January 2015, 中国纺织出版社 * |
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Application publication date: 20151223 |