CN105176796B - Vibration equipment of cell culture solution - Google Patents
Vibration equipment of cell culture solution Download PDFInfo
- Publication number
- CN105176796B CN105176796B CN201510622544.6A CN201510622544A CN105176796B CN 105176796 B CN105176796 B CN 105176796B CN 201510622544 A CN201510622544 A CN 201510622544A CN 105176796 B CN105176796 B CN 105176796B
- Authority
- CN
- China
- Prior art keywords
- cell culture
- culture fluid
- vibrating diaphragm
- vibratory equipment
- accommodating case
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000004113 cell culture Methods 0.000 title abstract 5
- 239000012930 cell culture fluid Substances 0.000 claims description 48
- 239000012528 membrane Substances 0.000 claims description 5
- 235000015097 nutrients Nutrition 0.000 claims description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims 2
- 229910052742 iron Inorganic materials 0.000 claims 1
- 230000035945 sensitivity Effects 0.000 abstract description 6
- 238000001514 detection method Methods 0.000 description 3
- 239000012530 fluid Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
Landscapes
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a vibrating device of cell culture solution, wherein the vibrating device of the cell culture solution comprises a signal generator and a loudspeaker electrically connected with the signal generator, the loudspeaker comprises a magnet, a basin frame and a vibrating diaphragm, and the vibrating diaphragm promotes the cell culture solution to vibrate. In the invention, the vibration of the vibrating diaphragm of the loudspeaker is utilized to promote the cell culture solution to vibrate, the sensitivity is higher, and the applicable frequency range is wider.
Description
Technical field
The present invention relates to a kind of vibratory equipment of cell culture fluid.
Background technology
Existing cell analysis technology, generally use detect fluid impedance value to analyze liquid, by analyzing fluid impedance
Change detect the growth conditions of cell.But same frequency of oscillation may correspond to two different impedance values.And if outer
When adding a frequency, detection is error-prone.And when impressed frequency is more than liquid resonant frequency, impedance value may be can't detect, survey
It is inadequate to measure sensitivity.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of vibratory equipment of cell culture fluid, the cell culture fluid
Vibratory equipment sensitivity is higher, and the scope of application is wider.
The present invention is to solve technical scheme used by above-mentioned technical problem：
A kind of vibratory equipment of cell culture fluid, wherein the vibratory equipment of the cell culture fluid include signal generator,
The loudspeaker electrically connected with the signal generator, the loudspeaker include magnet, frame and vibrating diaphragm, and the vibrating diaphragm promotes described
Cell culture fluid vibrates.
The invention has the advantages that vibration of membrane is shaken to promote cell culture fluid to vibrate using loudspeaker, sensitivity
It is higher, and it is wider to be applicable frequency range.
Preferably, the vibratory equipment of the cell culture fluid also includes being used for the accommodating case for containing the cell culture fluid,
The connector of the vibrating diaphragm is arranged at, the connector supports the accommodating case that the vibration of membrane of shaking is passed into the appearance
Cell culture fluid in case.
Preferably, the signal frequency range that the signal generator is sent is 20-6000 hertz.
Preferably, the vibratory equipment of the cell culture fluid also includes being used for the accommodating case for containing the cell culture fluid,
It is arranged at the connector of the vibrating diaphragm, the connector stretches into the accommodating case and contacts the cell culture fluid with by institute
State vibration of membrane of shaking and pass to cell culture fluid in the accommodating case.
Preferably, the connector is provided with a plurality of vibration heads, and the vibration head is fully inserted into the cell culture fluid
It is interior.
Preferably, the signal frequency range that the signal generator is sent is 6000-20000 hertz.
Preferably, the signal frequency that the signal generator is sent is 20-20000 hertz.
Brief description of the drawings
Purpose, technical scheme and the beneficial effect of invention described above can realize this hair by following
The detailed description of bright specific embodiment, describe in combination with accompanying drawing and clearly obtain.
Identical label and symbol in accompanying drawing and specification are used to represent identical or equivalent element.
Fig. 1 shows the schematic diagram of the vibratory equipment of cell culture fluid provided in an embodiment of the present invention.
Wherein：8. signal generator, 10. first loudspeakers, 12. first magnet, 14. first frame, 16. first shake
Film, 17. first accommodating cases, 18. first connectors, 19. second loudspeakers, 20. second magnet, 21. second frame,
22. the second vibrating diaphragm, 23. second accommodating cases, 24. second connectors, 26. vibration heads.
Embodiment
As shown in figure 1, a kind of vibratory equipment of cell culture fluid, including signal generator 8, it is electrically connected with signal generator 8
The first loudspeaker 10 connect, the first loudspeaker 10 include the first magnet 12, the first frame 14 and the first vibrating diaphragm 16, the first vibrating diaphragm 16
Cell culture fluid is promoted to vibrate.Signal generator 8 produces the signal of each frequency band, is delivered to the first loudspeaker 10, and first raises one's voice
First vibrating diaphragm 16 of device 10 vibrates, so as to promote cell culture fluid to vibrate.
The invention has the advantages that promote cell culture fluid using the vibration of the first vibrating diaphragm 16 of the first loudspeaker 10
Vibration, sensitivity is higher, and so as to which accuracy of detection is higher, and it is wider to be applicable frequency range.
In this preferred embodiment, vibration caused by the signal of low frequency, intermediate frequency and each frequency band of high frequency can be detected to thin
The influence of born of the same parents' nutrient solution.Specifically, the signal frequency that signal generator 8 is sent is 20-20000 hertz.
Further, the vibratory equipment of cell culture fluid also includes being used for the first accommodating case 17 for containing cell culture fluid,
The first connector 18 of the first vibrating diaphragm 16 is arranged at, the first connector 18 supports the first accommodating case 17 with shaking the first vibrating diaphragm 16
The dynamic cell culture fluid passed in the first accommodating case 17.Now, the first loudspeaker 10 is located at the lower section of cell culture fluid.For
Detect influence of the vibration to cell culture fluid caused by the signal of low and medium frequency.Specifically, the letter that now signal generator 8 is sent
Number frequency range is 20-6000 hertz.
In addition, to make the frequency segment limit that the equipment detects wider, the vibratory equipment of cell culture fluid is also raised including second
Sound device 19, the second loudspeaker 19 include the second magnet 20, the second frame 21 and the second vibrating diaphragm 22, the vibratory equipment of cell culture fluid
Also include being used for the second accommodating case 23 for containing cell culture fluid, be arranged at the second connector 24 of the second vibrating diaphragm 22, second connects
Fitting 24 stretches into 23 interior and exposing cell nutrient solution of the second accommodating case so that the vibration of the second vibrating diaphragm 22 directly is passed into the second appearance
Cell culture fluid in case 23.Specifically, the second connector 24 is provided with a plurality of vibration heads 26, vibration head 26 is fully inserted into
In cell culture fluid.
Now, the second loudspeaker 19 is located at the top of cell culture fluid.Shaken for detecting caused by high-frequency signal
The dynamic influence to cell culture fluid.Specifically, the signal frequency range that now signal generator 8 is sent is 6000-20000 hertz.
Because vibration is smaller caused by high-frequency signal, is lost to reduce, is accurately detected caused by certain high-frequency signal and shakes
The dynamic influence to cell culture fluid, vibration head 26 is directly protruding into cell culture fluid so that sensitivity is higher, and detection is more
Accurately.
The above example only present invention therein one, two kinds of embodiments, its description is more specific and detailed, but not
Therefore the limitation to the scope of the claims of the present invention can be interpreted as.It should be pointed out that come for one of ordinary skill in the art
Say, on the premise of the inventive concept of the present invention is not departed from, various modifications and improvements can be made, these belong to the present invention
Protection domain.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (5)
- A kind of 1. vibratory equipment of cell culture fluid, it is characterised in that：The vibratory equipment of the cell culture fluid is sent out including signal Raw device, the first loudspeaker and the second loudspeaker electrically connected with the signal generator, first loudspeaker include the first magnetic Iron, the first frame and the first vibrating diaphragm, second loudspeaker include the second magnet, the second frame and the second vibrating diaphragm, the cell The vibratory equipment of nutrient solution also includes being used for the first accommodating case for containing the cell culture fluid, is arranged at first vibrating diaphragm First connector, first connector support the accommodating case to pass to described first and hold the described first vibration of membrane of shaking Cell culture fluid in case, first vibrating diaphragm promote the cell culture fluid in first accommodating case to vibrate, the cell The vibratory equipment of nutrient solution also includes being used for the second accommodating case for containing the cell culture fluid, is arranged at second vibrating diaphragm Second connector, second connector stretch into second accommodating case and contact the cell culture fluid with by described the Two vibration of membranes of shaking pass to cell culture fluid in second accommodating case.
- 2. the vibratory equipment of cell culture fluid according to claim 1, it is characterised in that：What the signal generator was sent Signal frequency range is 20-6000 hertz.
- 3. the vibratory equipment of cell culture fluid according to claim 1, it is characterised in that：Second connector is provided with A plurality of vibration heads, the vibration head are fully inserted into the cell culture fluid of second accommodating case.
- 4. the vibratory equipment of cell culture fluid according to claim 1, it is characterised in that：What the signal generator was sent Signal frequency range is 6000-20000 hertz.
- 5. the vibratory equipment of cell culture fluid according to claim 1, it is characterised in that：What the signal generator was sent Signal frequency is 20-20000 hertz.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510622544.6A CN105176796B (en) | 2015-09-28 | 2015-09-28 | Vibration equipment of cell culture solution |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510622544.6A CN105176796B (en) | 2015-09-28 | 2015-09-28 | Vibration equipment of cell culture solution |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105176796A CN105176796A (en) | 2015-12-23 |
| CN105176796B true CN105176796B (en) | 2018-02-02 |
Family
ID=54899230
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510622544.6A Expired - Fee Related CN105176796B (en) | 2015-09-28 | 2015-09-28 | Vibration equipment of cell culture solution |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105176796B (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006119932A1 (en) * | 2005-05-09 | 2006-11-16 | Orion Diagnostica Oy | Sonication of a medium |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10108799A1 (en) * | 2001-02-19 | 2002-09-05 | Laser & Med Tech Gmbh | Method and device for the ultrasonic vaccination of biological cell material |
| US6739531B2 (en) * | 2001-10-04 | 2004-05-25 | Cepheid | Apparatus and method for rapid disruption of cells or viruses |
| CN204145742U (en) * | 2014-09-05 | 2015-02-04 | 寻乌县爱馨泰电子商贸有限公司 | A kind of motor-operated horn with vibration transmission mechanism |
-
2015
- 2015-09-28 CN CN201510622544.6A patent/CN105176796B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006119932A1 (en) * | 2005-05-09 | 2006-11-16 | Orion Diagnostica Oy | Sonication of a medium |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105176796A (en) | 2015-12-23 |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180202 |