CN105168255A - Zymolytic tuna blood oral liquid for antithrombotic and thrombolytic therapies and preparation method thereof - Google Patents
Zymolytic tuna blood oral liquid for antithrombotic and thrombolytic therapies and preparation method thereof Download PDFInfo
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Abstract
The invention discloses zymolytic tuna blood oral liquid for antithrombotic and thrombolytic therapies and a preparation method thereof. Tuna blood zymolyte extract is taken as a main raw material, and every 100mL of the oral liquid is prepared from the following components: 80 to 90 mL of zymolytic tuna blood concentrated liquor; 1 to 1.8g of citric acid; the balance of sterile water. The oral liquid prepared from the zymolytic tuna blood concentrated liquor is good in antithrombotic and thrombolytic effects, convenient and fast in application, low in price and safe and reliable, and is capable of eliminating the pain of a patient suffering from cardiovascular and cerebrovascular diseases of thromboembolism. The citric acid is capable of further improving the effect and result as well as the mouthfeel. Therefore, the zymolytic tuna blood oral liquid has the advantages of good antithrombotic and thrombolytic effects, convenience and quickness in application, low cost, safety and reliability and high productivity.
Description
Technical field
The present invention relates to a kind of thromboembolism preventing Thrombolytic agent and preparation method thereof, particularly relate to enzymolysis tuna blood thromboembolism preventing thrombolytic oral liquid and preparation method thereof.
Background technology
The mortality rate of world's cardiovascular and cerebrovascular disease occupy the first two position always, and the number of such disease is died from every year more than 1,500 ten thousand in the whole world, and wherein thromboembolia type cardiovascular and cerebrovascular disease M & M is the highest.According to Ministry of Public Health statistics, China dies from about 2,000,000 of cardiovascular and cerebrovascular disease every year, exceedes whole dead patient's 50%.In cardiovascular and cerebrovascular disease, significant proportion is thrombotic disease, and the highest with the sickness rate of thrombotic disease, disability rate and mortality rate, and its cause of disease is the formation of thrombosis.The most effective way for the treatment of of thrombotic disorders is thromboembolism treatment, and the mankind need every year for such disease pays huge expenses for medicine.
Though cause Intravascular Thrombus to be formed have many factors, the blood coagulation that thrombin brings out is thrombotic main trigger mechanism.Thrombosis produces the flowing of blood and hinders, and makes the thromboembolism that corresponding tissue, organ occur in various degree, afterwards can for want of oxygen and nutritional labeling and cause the appearance of a series of clinical symptoms:
If blood vessel embolism occurs in cerebral arteries, brain will because of the unexpected aphasia hemiplegia of infraction, even death; If blood vessel embolism occurs in cardiovascular significant points, heart will stop beating because blood supply interrupts, and causes sudden death etc.Thrombosis relevant disease onset is hidden, and fall ill hurried, the fatality rate that disables is high, and thrombosis is that cardiovascular and cerebrovascular disease causes dead, the residual arch-criminal of people.Current, treatment thromboembolia type cardiovascular and cerebrovascular disease mainly uses heparin series products both at home and abroad.But the curative effect of heparin is undesirable and side effect large, be badly in need of the product substituted.
Hirudin is class relative molecular mass about 7000 polypeptide in Hirudo salivary gland, research finds, the special effect inhibitor of hirudin thrombin, it is rich in the active site specific binding of basic amino acid mainly through the active site be made up of 3-4 acidic amino acid held near peptide chain C-and thrombin.Experimental result shows, hirudin can prevent new thrombosis, can also make established thromboembolism, all has fabulous preventive and therapeutic action to multiple thrombotic disease disease.But because hirudin is hydrolyzed by patient's gastrointestinal tract, can lose the effect of thromboembolism preventing, thrombolytic, although drug administration by injection curative effect is fine, registration can bring very large misery and inconvenience to patient repeatedly, is difficult to adapt to the needs that most of chronic height coagulates state patient long-term anticoagulant therapy.And Hirudo feeding cost is higher, hirudin extraction process is complicated, equipment investment is large, and injection price is higher, and the economic pressures that patient bears are large.
Tuna is one of important operation fingerling of world's deep-sea fishing, and according to FAO's statistics, world's tuna annual production, more than 6,000,000 tons, accounts for high sea fishery total output more than 70%, produces a large amount of tuna blood in the tuna course of processing.Research shows that in tuna blood, crude protein content is also higher, is good protein source.But the processed and applied situation of China's tuna blood is not only as people's will, and more than 90% is still dropped, and causes the significant wastage of tuna resources, also brings larger pressure to ecological environment.Therefore comprehensive high-efficiency utilizes tuna blood to have great importance.
China is still in the blank stage for the research of tuna blood thromboembolism preventing, thrombolytic, has no report at present for practicable enzymolysis process and separation and purification.And the Trionyx sinensis Wiegmann, mussel, mussel etc. reported at present for raw material prepare thromboembolism preventing, thrombolytic method and be not suitable for tuna blood for raw material prepares thromboembolism preventing, thrombolytic.
Summary of the invention
The object of the invention is to the enzymolysis tuna blood thromboembolism preventing thrombolytic oral liquid providing a kind of antithrombotic for prior art and thrombus is effective, convenient to use, cost is low, safe and reliable.
The present invention solves the problems of the technologies described above adopted technical scheme: enzymolysis tuna blood thromboembolism preventing thrombolytic oral liquid and preparation method thereof, comprise and adopt tuna blood enzymatic hydrolyzed extract to be primary raw material, wherein: the every 100mL of oral liquid is containing, for example lower component: the tuna hemoconcentration liquid 80-90mL after enzymolysis; Citric acid 1-1.8g; Sterilized water surplus.Oral liquid antithrombotic prepared by the tuna hemoconcentration liquid after enzymolysis and thrombus is effective, convenient to use, price is low, safe and reliable, thromboembolia type Patients with Cardiovascular/Cerebrovascular Diseases can be eliminated painful.Citric acid can further improving effect effect and mouthfeel.
For optimizing technique scheme, the measure taked also comprises: the every 100mL of oral liquid is also containing cyclamate 0.1-0.3g.Cyclamate, for improving mouthfeel, can avoid the negative effect to blood glucose simultaneously.Tuna hemoconcentration liquid after enzymolysis is prepared in the following way:
1), after tuna blood being removed raw meat, pH is regulated to be 9-11 with NaOH, for subsequent use after heat denatured;
2) will after step 1) process tuna blood HCl regulate pH to be 7-8, enzymolysis deactivation under certain condition, after being cooled to room temperature, centrifugal, vacuum concentration, obtains the tuna hemoconcentration liquid after enzymolysis.By regulating pH, can well effective ingredient be extracted, and simple to operate, with low cost.Step 1) removal agent of raw meat smell is No. 6 light gasoline; The volume ratio of removal agent of raw meat smell and tuna blood is 4-6:1; Step 2) in vacuum concentration be that enzymolysis clear liquid to be condensed under the vacuum condition of 85-90Kpa vigor be 390-400 antithrombase unit of activity.No. 6 light gasoline can retain the activity of finished product small-molecular peptides, play the effect improving effect.Step 1) regulates the process of pH to be 9-11 by the food stage NaOH solution adjusted to ph of 0.1-0.5mol/L, obtains the tuna hemodilution liquid of high ph-values; The process of heat denatured is by the tuna hemodilution liquid agitating heating 88-90 DEG C of high ph-values, maintains 25-35min, is cooled to room temperature.The use of too much NaOH should be avoided as far as possible, otherwise can affect the effectiveness of end-product.The cooperation of suitable acid extraction can obtain the high yield finished product with practical function.Step 2) regulate the process of pH to be use food stage HCl solution to be adjusted to pH value for 7-8 the tuna blood after step 1) process; Step 2) centrifugal condition is rotating speed 8000r/min-10000r/min, time 10-15min, temperature is 4-10 DEG C.Reply neutral semi-finished product, after lower temperature is centrifugal, relatively large effective ingredient can be obtained.
The present invention also provides a kind of productive rate high, simple to operate, with low cost, the preparation method of manufactured goods antithrombotic and effective, the safe and reliable enzymolysis tuna blood thromboembolism preventing thrombolytic oral liquid of thrombus.
And preparation method thereof.There are following steps:
1), after tuna blood being removed raw meat, pH is regulated to be 9-11 with NaOH, for subsequent use after heat denatured;
2) will after step 1) process tuna blood HCl regulate pH to be 7-8, enzymolysis deactivation under certain condition, after being cooled to room temperature, centrifugal, vacuum concentration, obtains the tuna hemoconcentration liquid after enzymolysis;
3) by step 2) enzymolysis after tuna hemoconcentration liquid add appropriate cyclamate, citric acid and sterilized water, after sterilizing and get final product.This method is simple to operate, with low cost.Antithrombase viability examination display finished product has good antithrombotic, thrombolytic effect.Removal agent of raw meat smell is No. 6 light gasoline; The volume ratio of removal agent of raw meat smell and tuna blood is 4-6:1; Step 1) regulates the process of pH to be 9-11 by the food stage NaOH solution adjusted to ph of 0.5mol/L, obtains the tuna hemodilution liquid of high ph-values; The process of heat denatured is by the tuna hemodilution liquid agitating heating 88-90 DEG C of high ph-values, maintains 25-35min, is cooled to room temperature; Step 2) regulate the process of pH to be use food stage HCl solution to be adjusted to pH value for 7-8 the tuna blood after step 1) process; Step 2) centrifugal condition is rotating speed 8000r/min-10000r/min, time 10-15min, temperature is 4-10 DEG C.Step 2) enzyme that adopts of enzymolysis is pepsin or trypsin or papain or its combination; The ratio of tuna blood and enzyme amount is 50-100mL:1g; During enzymolysis, condition is temperature 50-60 DEG C, time 2.5-3.5h; Deactivation condition is temperature 95-100 DEG C, time 10-15min.
Owing to present invention employs the every 100mL of oral liquid containing, for example lower component: the tuna hemoconcentration liquid 80-90mL after enzymolysis; Citric acid 1-1.8g; Sterilized water surplus.Oral liquid antithrombotic prepared by the tuna hemoconcentration liquid after enzymolysis and thrombus is effective, convenient to use, price is low, safe and reliable, thromboembolia type Patients with Cardiovascular/Cerebrovascular Diseases can be eliminated painful.Citric acid can further improving effect effect and mouthfeel.Thus the advantage that the present invention has antithrombotic and thrombus is effective, convenient to use, cost is low, safe and reliable, productive rate is high.
Detailed description of the invention
Below in conjunction with attached embodiment, the present invention is described in further detail.
Embodiment 1: enzymolysis tuna blood thromboembolism preventing thrombolytic oral liquid and preparation method thereof, comprises and adopts tuna blood enzymatic hydrolyzed extract to be primary raw material, wherein: the every 100mL of oral liquid is containing, for example lower component: the tuna hemoconcentration liquid 80-90mL after enzymolysis; Citric acid 1-1.8g; Sterilized water surplus.Oral liquid antithrombotic prepared by the tuna hemoconcentration liquid after enzymolysis and thrombus is effective, convenient to use, price is low, safe and reliable, thromboembolia type Patients with Cardiovascular/Cerebrovascular Diseases can be eliminated painful.Citric acid can further improving effect effect and mouthfeel.The every 100mL of oral liquid is also containing cyclamate 0.1-0.3g.Cyclamate, for improving mouthfeel, can avoid the negative effect to blood glucose simultaneously.Tuna hemoconcentration liquid after enzymolysis is prepared in the following way:
1), after tuna blood being removed raw meat, pH is regulated to be 9-11 with NaOH, for subsequent use after heat denatured;
2) will after step 1) process tuna blood HCl regulate pH to be 7-8, enzymolysis deactivation under certain condition, after being cooled to room temperature, centrifugal, vacuum concentration, obtains the tuna hemoconcentration liquid after enzymolysis.By regulating pH, can well effective ingredient be extracted, and simple to operate, with low cost.Step 1) removal agent of raw meat smell is No. 6 light gasoline; The volume ratio of removal agent of raw meat smell and tuna blood is 4-6:1; Step 2) in vacuum concentration be that enzymolysis clear liquid to be condensed under the vacuum condition of 85-90Kpa vigor be 390-400 antithrombase unit of activity.No. 6 light gasoline can retain the activity of finished product small-molecular peptides, play the effect improving effect.Step 1) regulates the process of pH to be 9-11 by the food stage NaOH solution adjusted to ph of 0.1-0.5mol/L, obtains the tuna hemodilution liquid of high ph-values; The process of heat denatured is by the tuna hemodilution liquid agitating heating 88-90 DEG C of high ph-values, maintains 25-35min, is cooled to room temperature.The use of too much NaOH should be avoided as far as possible, otherwise can affect the effectiveness of end-product.The cooperation of suitable acid extraction can obtain the high yield finished product with practical function.Step 2) regulate the process of pH to be use food stage HCl solution to be adjusted to pH value for 7-8 the tuna blood after step 1) process; Step 2) centrifugal condition is rotating speed 8000r/min-10000r/min, time 10-15min, temperature is 4-10 DEG C.Reply neutral semi-finished product, after lower temperature is centrifugal, relatively large effective ingredient can be obtained.There are following steps:
1), after tuna blood being removed raw meat, pH is regulated to be 9-11 with NaOH, for subsequent use after heat denatured;
2) will after step 1) process tuna blood HCl regulate pH to be 7-8, enzymolysis deactivation under certain condition, after being cooled to room temperature, centrifugal, vacuum concentration, obtains the tuna hemoconcentration liquid after enzymolysis;
3) by step 2) enzymolysis after tuna hemoconcentration liquid add appropriate cyclamate, citric acid and sterilized water, after sterilizing and get final product.This method is simple to operate, with low cost.Antithrombase viability examination display finished product has good antithrombotic, thrombolytic effect.Removal agent of raw meat smell is No. 6 light gasoline; The volume ratio of removal agent of raw meat smell and tuna blood is 4-6:1; Step 1) regulates the process of pH to be 9-11 by the food stage NaOH solution adjusted to ph of 0.5mol/L, obtains the tuna hemodilution liquid of high ph-values; The process of heat denatured is by the tuna hemodilution liquid agitating heating 88-90 DEG C of high ph-values, maintains 25-35min, is cooled to room temperature; Step 2) regulate the process of pH to be use food stage HCl solution to be adjusted to pH value for 7-8 the tuna blood after step 1) process; Step 2) centrifugal condition is rotating speed 8000r/min-10000r/min, time 10-15min, temperature is 4-10 DEG C.Step 2) enzyme that adopts of enzymolysis is pepsin or trypsin or papain or its combination; The ratio of tuna blood and enzyme amount is 50-100mL:1g; During enzymolysis, condition is temperature 50-60 DEG C, time 2.5-3.5h; Deactivation condition is temperature 95-100 DEG C, time 10-15min.Introduce an example below to be further described:
(1) depletion marlin blood 100mL, after No. 6 light gasoline adding 400mL remove raw meat, the food stage NaOH adding 0.5% raises pH to 9, obtains the tuna blood under high pH;
(2) be heated to 88 DEG C by the tuna blood under high pH and heating tank, maintain 25min, make the abundant degeneration of albumen;
(3) use the food stage HCl solution of 0.5% to regulate pH to 7 the high pH tuna blood of fully degeneration, obtain degeneration tuna diluent;
(4) get above-mentioned tuna diluent and add 1g trypsin, at 55 DEG C after enzymolysis 3h, rapid temperature increases to 95 DEG C, maintains 10min;
(5) be 8000r/min by enzymolysis solution at rotating speed, temperature is centrifugal 10min at 4 DEG C, gets supernatant;
(6) enzymolysis supernatant is concentrated under the vacuum condition of 90Kpa, obtain thromboembolism preventing, thrombolytic oral liquid mother solution;
(7) in mother solution, add the cyclamate of 0.1g respectively, the citric acid of 1g and the sterilized water of 10mL, obtain enzymolysis tuna blood thromboembolism preventing, thrombolytic oral liquid.
Embodiment 2:
(1) depletion marlin blood 100mL, after No. 6 light gasoline adding 400mL remove raw meat, the food stage NaOH adding 0.5% raises pH to 10, obtains the tuna blood under high pH;
(2) be heated to 90 DEG C by the tuna blood under high pH and heating tank, maintain 30min, make the abundant degeneration of albumen;
(3) use the food stage HCl solution of 0.5% to regulate pH to 7 the high pH tuna blood of fully degeneration, obtain degeneration tuna diluent;
(4) get above-mentioned tuna diluent and add 0.5g trypsin, at 55 DEG C after enzymolysis 3h, rapid temperature increases to 95 DEG C, maintains 10min;
(5) be 8000r/min by enzymolysis solution at rotating speed, temperature is centrifugal 10min at 4 DEG C, gets supernatant;
(6) enzymolysis supernatant is concentrated under the vacuum condition of 90Kpa, obtain thromboembolism preventing, thrombolytic oral liquid mother solution;
(7) in mother solution, add the cyclamate of 0.1g respectively, the citric acid of 1g and the sterilized water of 10mL, obtain enzymolysis tuna blood thromboembolism preventing, thrombolytic oral liquid.
Embodiment 3:
(1) depletion marlin blood 100mL, after No. 6 light gasoline adding 500mL remove raw meat, the food stage NaOH adding 0.5% raises pH to 11, obtains the tuna blood under high pH;
(2) be heated to 88 DEG C by the tuna blood under high pH and heating tank, maintain 25min, make the abundant degeneration of albumen;
(3) use the food stage HCl solution of 0.5% to regulate pH to 7 the high pH tuna blood of fully degeneration, obtain degeneration tuna diluent;
(4) get above-mentioned tuna diluent and add 1g pepsin, at 55 DEG C after enzymolysis 3h, rapid temperature increases to 95 DEG C, maintains 10min;
(5) be 9000r/min by enzymolysis solution at rotating speed, temperature is centrifugal 10min at 4 DEG C, gets supernatant;
(6) enzymolysis supernatant is concentrated under the vacuum condition of 90Kpa, obtain thromboembolism preventing, thrombolytic oral liquid mother solution;
(7) in mother solution, add the cyclamate of 0.1g respectively, the citric acid of 1g and the sterilized water of 10mL, obtain enzymolysis tuna blood thromboembolism preventing, thrombolytic oral liquid.
Embodiment 4:
(1) depletion marlin blood 100mL, after No. 6 light gasoline adding 400mL remove raw meat, the food stage NaOH adding 0.5% raises pH to 9, obtains the tuna blood under high pH;
(2) be heated to 88 DEG C by the tuna blood under high pH and heating tank, maintain 25min, make the abundant degeneration of albumen;
(3) use the food stage HCl solution of 0.5% to regulate pH to 7 the high pH tuna blood of fully degeneration, obtain degeneration tuna diluent;
(4) get above-mentioned tuna diluent and add 1g trypsin, at 60 DEG C after enzymolysis 3.5h, rapid temperature increases to 100 DEG C, maintains 10min;
(5) be 8000r/min by enzymolysis solution at rotating speed, temperature is centrifugal 10min at 4 DEG C, gets supernatant;
(6) enzymolysis supernatant is concentrated under the vacuum condition of 90Kpa, obtain thromboembolism preventing, thrombolytic oral liquid mother solution;
(7) in mother solution, add the cyclamate of 0.1g respectively, the citric acid of 1g and the sterilized water of 10mL, obtain enzymolysis tuna blood thromboembolism preventing, thrombolytic oral liquid.
Embodiment 5:
(1) depletion marlin blood 100mL, after No. 6 light gasoline adding 400mL remove raw meat, the food stage NaOH adding 0.5% raises pH to 9, obtains the tuna blood under high pH;
(2) be heated to 88 DEG C by the tuna blood under high pH and heating tank, maintain 25min, make the abundant degeneration of albumen;
(3) use the food stage HCl solution of 0.5% to regulate pH to 7 the high pH tuna blood of fully degeneration, obtain degeneration tuna diluent;
(4) get above-mentioned tuna diluent and add 0.8g trypsin, at 55 DEG C after enzymolysis 3.5h, rapid temperature increases to 100 DEG C, maintains 15min;
(5) be 10000r/min by enzymolysis solution at rotating speed, temperature is centrifugal 15min at 4 DEG C, gets supernatant;
(6) enzymolysis supernatant is concentrated under the vacuum condition of 90Kpa, obtain thromboembolism preventing, thrombolytic oral liquid mother solution;
(7) in mother solution, add the cyclamate of 0.1g respectively, the citric acid of 1g and the sterilized water of 15mL, obtain enzymolysis tuna blood thromboembolism preventing, thrombolytic oral liquid.
Thromboembolism preventing, thrombolytic oral liquid Performance Detection: carry out antithrombase viability examination according to the experimental technique that People's Republic of China's standard health (functional) food universal standard (GB16740-1997) adopts to enzymolysis tuna blood thromboembolism preventing, thrombolytic oral liquid, detailed results is as shown in the table;
| Embodiment | The mass fraction (%) of hydrolase polypeptide thing total amount | Antithrombase vigor (ATU/mL) |
| 1 | 93.58 | 375.17 |
| 2 | 95.67 | 372.43 |
| 3 | 94.32 | 379.2 |
| 4 | 92.11 | 378.14 |
| 5 | 94.12 | 376.57 |
Although describe the present invention in conjunction with preferred embodiment; so itself and be not used to limit the present invention; any those skilled in the art; without departing from the spirit and scope of the present invention; can implement various change, the displacement of coordinate and amendment here to the theme listed, therefore protection scope of the present invention be as the criterion when the scope limited depending on proposed claim.
Claims (9)
1. enzymolysis tuna blood thromboembolism preventing thrombolytic oral liquid, comprises and adopts tuna blood enzymatic hydrolyzed extract to be primary raw material, it is characterized in that: the every 100mL of described oral liquid is containing, for example lower component:
Tuna hemoconcentration liquid 80-90mL after enzymolysis;
Citric acid 1-1.8g;
Sterilized water surplus.
2. enzymolysis tuna blood thromboembolism preventing thrombolytic oral liquid according to claim 1, is characterized in that: the every 100mL of described oral liquid is also containing cyclamate 0.1-0.3g.
3. enzymolysis tuna blood thromboembolism preventing thrombolytic oral liquid according to claim 1, is characterized in that: the tuna hemoconcentration liquid after described enzymolysis is prepared in the following way:
1), after tuna blood being removed raw meat, pH is regulated to be 9-11 with NaOH, for subsequent use after heat denatured;
2) will after step 1) process tuna blood HCl regulate pH to be 7-8, enzymolysis deactivation under certain condition, after being cooled to room temperature, centrifugal, vacuum concentration, obtains the tuna hemoconcentration liquid after enzymolysis.
4. enzymolysis tuna blood thromboembolism preventing thrombolytic oral liquid according to claim 3, is characterized in that: the removal agent of raw meat smell described in step 1) is No. 6 light gasoline; The volume ratio of described removal agent of raw meat smell and described tuna blood is 4-6:1; Step 2) described in vacuum concentration be that enzymolysis clear liquid to be condensed under the vacuum condition of 85-90Kpa vigor be 390-400 antithrombase unit of activity.
5. enzymolysis tuna blood thromboembolism preventing thrombolytic oral liquid according to claim 3, is characterized in that: the process of the adjustment pH described in step 1) is be 9-11 by the food stage NaOH solution adjusted to ph of 0.1-0.5mol/L, obtains the tuna hemodilution liquid of high ph-values; The process of described heat denatured is by the tuna hemodilution liquid agitating heating 88-90 DEG C of described high ph-values, maintains 25-35min, is cooled to room temperature.
6. the preparation method of enzymolysis tuna blood thromboembolism preventing thrombolytic oral liquid, is characterized in that: step 2) described in the process of adjustment pH be use food stage HCl solution to be adjusted to pH value for 7-8 the tuna blood after step 1) process; Step 2) described in centrifugal condition be rotating speed 8000r/min-10000r/min, time 10-15min, temperature is 4-10 DEG C.
7. the preparation method of enzymolysis tuna blood thromboembolism preventing thrombolytic oral liquid according to claim 6, is characterized in that: have following steps:
1), after tuna blood being removed raw meat, pH is regulated to be 9-11 with NaOH, for subsequent use after heat denatured;
2) will after step 1) process tuna blood HCl regulate pH to be 7-8, enzymolysis deactivation under certain condition, after being cooled to room temperature, centrifugal, vacuum concentration, obtains the tuna hemoconcentration liquid after enzymolysis;
3) by step 2) enzymolysis after tuna hemoconcentration liquid add appropriate cyclamate, citric acid and sterilized water, after sterilizing and get final product.
8. the preparation method of enzymolysis tuna blood thromboembolism preventing thrombolytic oral liquid according to claim 7, is characterized in that: described removal agent of raw meat smell is No. 6 light gasoline; The volume ratio of described removal agent of raw meat smell and tuna blood is 4-6:1; The process of the adjustment pH described in step 1) is be 9-11 by the food stage NaOH solution adjusted to ph of 0.5mol/L, obtains the tuna hemodilution liquid of high ph-values; The process of described heat denatured is by the tuna hemodilution liquid agitating heating 88-90 DEG C of described high ph-values, maintains 25-35min, is cooled to room temperature; Step 2) described in the process of adjustment pH be use food stage HCl solution to be adjusted to pH value for 7-8 the tuna blood after step 1) process; Step 2) described in centrifugal condition be rotating speed 8000r/min-10000r/min, time 10-15min, temperature is 4-10 DEG C.
9. the preparation method of enzymolysis tuna blood thromboembolism preventing thrombolytic oral liquid according to claim 7, is characterized in that: step 2) described in the enzyme that adopts of enzymolysis be pepsin or trypsin or papain or its combination; Described tuna blood and the ratio of enzyme amount are 50-100mL:1g; During described enzymolysis, condition is temperature 50-60 DEG C, time 2.5-3.5h; Described deactivation condition is temperature 95-100 DEG C, time 10-15min.
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| CN104878063A (en) * | 2015-06-10 | 2015-09-02 | 广西盟展鳄鱼科技开发有限公司 | Crocodilian serum protein with antineoplastic function and preparation method thereof |
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Application publication date: 20151223 |