CN105158233B - A kind of method stable based on the real-time Raman spectrum of External reference thing method and photobleaching - Google Patents
A kind of method stable based on the real-time Raman spectrum of External reference thing method and photobleaching Download PDFInfo
- Publication number
- CN105158233B CN105158233B CN201510638194.2A CN201510638194A CN105158233B CN 105158233 B CN105158233 B CN 105158233B CN 201510638194 A CN201510638194 A CN 201510638194A CN 105158233 B CN105158233 B CN 105158233B
- Authority
- CN
- China
- Prior art keywords
- optical fiber
- external reference
- measurement
- photobleaching
- raman spectrum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a kind of method stable based on the real-time Raman spectrum of External reference thing method and photobleaching；The first step, system building, second step, to product to be tested photobleaching before measurement；3rd step, static measurement and dynamic measure；The method stable based on the real-time Raman spectrum of External reference thing method and photobleaching of the present invention, by optical fiber multiplexing method, using wavelength-division multiplex technique, simultaneous transmission two-way raman spectral signal in a fiber；By two-way optical fiber, realize that real-time Raman spectrum is stable by External reference method as External reference signal as measurement signal, another way all the way；Before measuring, by additional laser irradiation device, by sample irradiation certain time, make the fluorescent weakening of sample simultaneously；Improve spectrum-stable；Finally detect when in measurement process mobile example or laser, to improve the heat transfer of sample；Realize the spectrum-stable in detection process.
Description
Technical field
It is more particularly to a kind of based on External reference thing method and photobleaching the present invention relates to a kind of Raman spectrum antihunt means
The stable method of real-time Raman spectrum, belongs to raman spectroscopy measurement technical field.
Background technology
Raman spectrum is a kind of scattering spectrum；Raman spectrum analysis method is found based on India's scientist's C.V. Ramans
Raman scattering effect, information in terms of a pair scattering spectrum different from incident light frequency is analyzed to obtain molecular vibration, rotation,
And applied to a kind of analysis method of molecular structure research, a kind of analysis means of testing of the Raman spectrum as material molecular structure
And be widely used, after nineteen sixty First laser comes out, the introducing of LASER Light Source, the raising of Technique of Weak Signal Detection
And computer application, Raman spectrum analysis is obtained very big progress in many application fields；Raman spectra detection process exists
The problem run into material detection is easily interfered in measurement process, easily occurs distortion after being disturbed, simultaneously meeting
Cause raman spectral signal weak, material can not be identified for the Raman spectrum measured.
The content of the invention
(1) technical problems to be solved
To solve the above problems, the present invention proposes a kind of real-time Raman spectrum based on External reference thing method and photobleaching
Stable method, it is combined by fiber multiplex and external reference method, realizes signal stabilization.
(2) technical scheme
The method stable based on the real-time Raman spectrum of External reference thing method and photobleaching of the present invention, methods described include
Following steps：
The first step, system building, screen pipe is introduced by the way that optical fiber and External reference optical fiber will be measured, is installed in screen pipe
One clear glass and a reference member；Measurement optical fiber is coordinated with clear glass and installed；Reference member and External reference optical fiber are coordinated and pacified
Dress；And screen pipe is placed in a container；Then measurement optical fiber and the External reference optical fiber other end are incorporated into Fu by joint
Leaf transformation-Raman spectrometer；Then one additional laser irradiation device, and additional laser irradiation dress are installed in container top or outside
Put face product to be tested；Finally, one high-precision head is installed in container bottom；
Second step, to product to be tested photobleaching before measurement；
Additional laser irradiation device is opened, and ensures its launch point face product to be tested, its bleaching process is no less than 5s；
3rd step, static measurement and dynamic measure,
(1) continuously the Raman spectrum of determinand is measured to gather two groups by measuring optical fiber and External reference optical fiber
Multiple raman spectral signals；
(2) fiber multiplex is used, using wavelength-division multiplex technique, simultaneous transmission two-way Raman spectrum is believed in a fiber
Number；
(3) two groups of raman spectral signals of feeding are pre-processed by Fourier transform-Raman spectra instrument；
(4) using the signal that External reference optical fiber is sent into as reference, measurement optical fiber is sent into signal as actual measurement Raman spectrum letter
Number proofreaded and signal transacting；
(5) determinand is identified the process signal completed based on previous step；
(6) head is opened, it is changed an angle and continues to detect.
Further, the measurement optical fiber is identical with External reference collecting fiber condition.
Further, the dynamic measurement forms discrete magnitude datagram by multigroup static measurement and formed.
Beneficial effect
Compared with prior art, the side stable based on the real-time Raman spectrum of External reference thing method and photobleaching of the invention
Method, by optical fiber multiplexing method, using wavelength-division multiplex technique, simultaneous transmission two-way raman spectral signal in a fiber；Pass through two
Road optical fiber, realize that real-time Raman spectrum is steady by External reference method as External reference signal as measurement signal, another way all the way
It is fixed；Before measuring, by additional laser irradiation device, by sample irradiation certain time, make the fluorescent weakening of sample simultaneously；Improve
Spectrum-stable；Finally detect when in measurement process mobile example or laser, to improve the heat transfer of sample；Realize detection process
In spectrum-stable.
Embodiment
The present invention is included such as based on the stable method of the real-time Raman spectrum of External reference thing method and photobleaching, methods described
Lower step：
Methods described comprises the following steps：
The first step, system building, screen pipe is introduced by the way that optical fiber and External reference optical fiber will be measured, is installed in screen pipe
One clear glass and a reference member；Measurement optical fiber is coordinated with clear glass and installed；Reference member and External reference optical fiber are coordinated and pacified
Dress；And screen pipe is placed in a container；Then measurement optical fiber and the External reference optical fiber other end are incorporated into Fu by joint
Leaf transformation-Raman spectrometer；Then one additional laser irradiation device, and additional laser irradiation dress are installed in container top or outside
Put face product to be tested；Finally, one high-precision head is installed in container bottom；
Second step, to product to be tested photobleaching before measurement；
Additional laser irradiation device is opened, and ensures its launch point face product to be tested, its bleaching process is no less than 5s；
3rd step, static measurement and dynamic measure,
(1) continuously the Raman spectrum of determinand is measured to gather two groups by measuring optical fiber and External reference optical fiber
Multiple raman spectral signals；
(2) fiber multiplex is used, using wavelength-division multiplex technique, simultaneous transmission two-way Raman spectrum is believed in a fiber
Number；
(3) two groups of raman spectral signals of feeding are pre-processed by Fourier transform-Raman spectra instrument；
(4) using the signal that External reference optical fiber is sent into as reference, measurement optical fiber is sent into signal as actual measurement Raman spectrum letter
Number proofreaded and signal transacting；
(5) determinand is identified the process signal completed based on previous step；
(6) head is opened, it is changed an angle and continues to detect.
Wherein, the measurement optical fiber is identical with External reference collecting fiber condition；The dynamic measurement is by multigroup static measurement
Form discrete magnitude datagram composition.
The method stable based on the real-time Raman spectrum of External reference thing method and photobleaching of the present invention, passes through optical fiber multiplexing
Method, using wavelength-division multiplex technique, simultaneous transmission two-way raman spectral signal in a fiber；Pass through two-way optical fiber, all the way conduct
Measurement signal, another way realize that real-time Raman spectrum is stable as External reference signal by External reference method；Measuring simultaneously
Before, by additional laser irradiation device, by sample irradiation certain time, make the fluorescent weakening of sample；Improve spectrum-stable；Finally
During detection in measurement process mobile example or laser, to improve the heat transfer of sample；Realize the spectrum-stable in detection process.
The above-described embodiments are merely illustrative of preferred embodiments of the present invention, not to the structure of the present invention
Think and scope is defined.On the premise of design concept of the present invention is not departed from, technology of the ordinary people in the field to the present invention
The all variations and modifications that scheme is made, all should drop into protection scope of the present invention, the claimed technology contents of the present invention,
All record in detail in the claims.
Claims (3)
- A kind of 1. method stable based on the real-time Raman spectrum of External reference thing method and photobleaching, it is characterised in that：The side Method comprises the following steps：The first step, system building, a screen pipe is introduced by the way that optical fiber and External reference optical fiber will be measured, it is saturating that one is installed in screen pipe Bright glass and a reference member；Measurement optical fiber is coordinated with clear glass and installed；Reference member and External reference optical fiber are coordinated and installed；And Screen pipe is placed in a container；Then measurement optical fiber and the External reference optical fiber other end are incorporated into Fourier by joint to become Change-Raman spectrometer；Then one additional laser irradiation device is installed in container top or outside, and additional laser irradiation device is just To product to be tested；Finally, one high-precision head is installed in container bottom；Second step, to product to be tested photobleaching before measurement；Additional laser irradiation device is opened, and ensures its launch point face product to be tested, its bleaching process is no less than 5s；3rd step, static measurement and dynamic measure,(1) by measure optical fiber and External reference optical fiber continuously the Raman spectrum of determinand is measured it is multiple to gather two groups Raman spectral signal；(2) fiber multiplex is used, using wavelength-division multiplex technique, simultaneous transmission two-way raman spectral signal in a fiber；(3) two groups of raman spectral signals of feeding are pre-processed by Fourier transform-Raman spectra instrument；(4) using the signal that External reference optical fiber is sent into as reference, measurement optical fiber is sent into signal and entered as actual measurement raman spectral signal Row check and correction and signal transacting；(5) determinand is identified the process signal completed based on previous step；(6) head is opened, it is changed an angle and continues to detect.
- 2. the method stable based on the real-time Raman spectrum of External reference thing method and photobleaching according to claim 1, its It is characterised by：The measurement optical fiber is identical with External reference collecting fiber condition.
- A kind of 3. side stable based on the real-time Raman spectrum of External reference thing method and photobleaching according to claim 1 Method, it is characterised in that dynamic measurement forms discrete magnitude datagram by multigroup static measurement and formed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510638194.2A CN105158233B (en) | 2015-09-24 | 2015-09-24 | A kind of method stable based on the real-time Raman spectrum of External reference thing method and photobleaching |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510638194.2A CN105158233B (en) | 2015-09-24 | 2015-09-24 | A kind of method stable based on the real-time Raman spectrum of External reference thing method and photobleaching |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105158233A CN105158233A (en) | 2015-12-16 |
| CN105158233B true CN105158233B (en) | 2017-12-29 |
Family
ID=54799166
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510638194.2A Expired - Fee Related CN105158233B (en) | 2015-09-24 | 2015-09-24 | A kind of method stable based on the real-time Raman spectrum of External reference thing method and photobleaching |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105158233B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111337446A (en) * | 2020-05-08 | 2020-06-26 | 宁波大学 | Biosensor based on chalcogenide glass optical fiber and preparation method thereof |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1149334A (en) * | 1994-05-27 | 1997-05-07 | 伊斯特曼化学公司 | Raman spectrometry apparatus and method |
| US6205265B1 (en) * | 1998-05-29 | 2001-03-20 | Litton Systems, Inc. | Apparatus and method for isolated photobleaching fiber optic rotation sensor coils |
| CN1105296C (en) * | 1996-08-22 | 2003-04-09 | 伊斯曼化学公司 | On-line quantitative analysis of chemical compositions by raman sepectrometry |
| CN1842696A (en) * | 2003-06-27 | 2006-10-04 | 坎米马吉公司 | Wide field method for detecting pathogenic microorganisms |
| CN101453948A (en) * | 2006-06-16 | 2009-06-10 | C8麦迪森瑟斯公司 | Reduction in scattering from a turbid medium by photo-bleaching |
| CN102062649A (en) * | 2010-11-26 | 2011-05-18 | 中国计量学院 | Dual wavelength light source self-correcting distributed optical fiber Raman temperature sensor for optical fiber Raman frequency shifter |
| CN102121902A (en) * | 2011-03-15 | 2011-07-13 | 南京工业大学 | Online Raman spectrometer correction device and correction method thereof |
| CN201909687U (en) * | 2010-12-23 | 2011-07-27 | 上海华魏光纤传感技术有限公司 | Double-end double-wavelength self-compensating distributed optical fiber temperature sensor |
| CN103411675A (en) * | 2013-07-18 | 2013-11-27 | 华中科技大学 | Excited Brillouin scattering gain spectrum measuring method and system thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009011565A1 (en) * | 2007-07-17 | 2009-01-22 | Erasmus University Medical Center Rotterdam | Method for typing and identification of micro-organisms |
-
2015
- 2015-09-24 CN CN201510638194.2A patent/CN105158233B/en not_active Expired - Fee Related
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1149334A (en) * | 1994-05-27 | 1997-05-07 | 伊斯特曼化学公司 | Raman spectrometry apparatus and method |
| CN1105296C (en) * | 1996-08-22 | 2003-04-09 | 伊斯曼化学公司 | On-line quantitative analysis of chemical compositions by raman sepectrometry |
| US6205265B1 (en) * | 1998-05-29 | 2001-03-20 | Litton Systems, Inc. | Apparatus and method for isolated photobleaching fiber optic rotation sensor coils |
| CN1842696A (en) * | 2003-06-27 | 2006-10-04 | 坎米马吉公司 | Wide field method for detecting pathogenic microorganisms |
| CN101453948A (en) * | 2006-06-16 | 2009-06-10 | C8麦迪森瑟斯公司 | Reduction in scattering from a turbid medium by photo-bleaching |
| CN102062649A (en) * | 2010-11-26 | 2011-05-18 | 中国计量学院 | Dual wavelength light source self-correcting distributed optical fiber Raman temperature sensor for optical fiber Raman frequency shifter |
| CN201909687U (en) * | 2010-12-23 | 2011-07-27 | 上海华魏光纤传感技术有限公司 | Double-end double-wavelength self-compensating distributed optical fiber temperature sensor |
| CN102121902A (en) * | 2011-03-15 | 2011-07-13 | 南京工业大学 | Online Raman spectrometer correction device and correction method thereof |
| CN103411675A (en) * | 2013-07-18 | 2013-11-27 | 华中科技大学 | Excited Brillouin scattering gain spectrum measuring method and system thereof |
Non-Patent Citations (2)
| Title |
|---|
| Is photobleaching necessary for Raman imaging of bone tissue using a green laser;Kurtulus Golcuk et al.;《Biochemica et Biophysica Acta-Biomembranes》;20060731;第1758卷(第7期);第868-873页 * |
| Photobleaching as a useful technique in reducing of fluorescence in Raman spectra of blue automobile paint samples;Janina Ziebe-Palus et al.;《Vibrational Spectroscopy》;20140930;第74卷;第6-12页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105158233A (en) | 2015-12-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Le Losq et al. | Determination of water content in silicate glasses using Raman spectrometry: Implications for the study of explosive volcanism | |
| CN105181672B (en) | A kind of Raman spectrum wave number and intensity real-time correction method | |
| CN101813623B (en) | Scatterer multi-dimensional spectral measuring unit and method | |
| WO2018018838A1 (en) | Raman spectrum analysis-based method for quickly detecting quality of coal | |
| CN101793678A (en) | Spectrum measuring device and method of scattering substance of sample cell with isosceles triangle cross section | |
| CN205607531U (en) | Portable dual wavelength fluorescence raman spectrum detection system that disappears | |
| CN102033048A (en) | Measuring method of absorption spectrum for eliminating ambient stray light interference | |
| CN102121902B (en) | Online Raman spectrometer correction device and correction method thereof | |
| CN104122363B (en) | A kind of assay method of methylcobalamin tablet related substance | |
| CN106500844B (en) | A kind of six passages divide amplitude high speed Stokes polarimeter and its measurement method of parameters | |
| CN205139006U (en) | Dew point measurement device based on laser spectrum absorbs detection | |
| CN105158233B (en) | A kind of method stable based on the real-time Raman spectrum of External reference thing method and photobleaching | |
| CN107991270A (en) | Blood species discrimination system based on coherent antistockes Raman spectroscopy | |
| CN101477051A (en) | Raman spectrum method and reagent kit used for on-site fast detection of melamine in liquid milk | |
| CN107831133A (en) | A kind of large-scale melon and fruit inside quality near infrared spectrum on-line detecting system and implementation method | |
| CN110535526A (en) | A kind of fiber-optic communications traffic loss test method | |
| CN105548057A (en) | Flue gas analysis and measurement method implemented through ultraviolet spectrum | |
| Choi et al. | Highly sensitive and accurate estimation of bloodstain age using smartphone | |
| CN215115874U (en) | Near-infrared quality detection system of different size fruits of self-adaptation | |
| CN207457076U (en) | A kind of tealeaves heavy metal analysis device | |
| Li et al. | Effects of temperature and wavelength choice on in-situ dissolution test of Cimetidine tablets | |
| CN106989905A (en) | A kind of multi-functional detection method and apparatus of luminescent panel | |
| Azan et al. | Monitoring the molecular composition of live cells exposed to electric pulses via label-free optical methods | |
| Yang et al. | Low-frequency vibrational modes of dl-homocysteic acid and related compounds | |
| CN103163579B (en) | Liquid identifying device and liquid identifying method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171229 Termination date: 20210924 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |