CN105158146B - A kind of flow cytometer and DPCR instrument addressable LED illumination array - Google Patents
A kind of flow cytometer and DPCR instrument addressable LED illumination array Download PDFInfo
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Abstract
The invention discloses a kind of flow cytometer and DPCR instrument addressable LED illumination array, including illumination unit, for sending exciting light at the flow chamber outlet port to flow cytometer or at the filter of DPCR instrument, excitation tested cell, particle or nucleic acid molecules send fluorescence；Bonding units, electric connection is established by using bonding method between illumination unit and driving unit；Driving unit, for being scanned under the control of the control unit according to clock frequency and driving illumination unit to shine；Control unit, for storing and performing the control program of illumination unit set in advance, control unit, which performs control program, makes illumination unit send exciting light according to the requirement of control program set in advance.
Description
Technical field
The present invention relates to cell and genetic engineering, especially, is related to a kind of flow cytometer and DPCR instrument addressables LED
Irradiate array.
Background technology
At present, made mostly using halogen lamp, laser, LED (light emitting diode) in many biomedical instrument courses of work
Sample is excited for light source, so as to achieve the purpose that tracing detection cell or particle.Existing Flow Cytometry and DPCR (numeral
PCR) technology is just respectively generally using laser beam and halogen lamp as excitation source.
Halogen lamp is a kind of typical thermal light source, and principle is the injection halogen gas such as iodine or bromine in light bulb, at high temperature,
The tungsten filament of distillation carries out chemical action with halogen, and tungsten after cooling can be set on tungsten filament again, form the circulation of balance.Halogen
Although lamp is wide as excitation source spectral region, brightness is high, and electro-optical efficiency is low, and (generally 30%, remainder is with warm
Energy form is scattered and disappeared), energy consumption.Halogen lamp is made of tungsten filament, is enclosed in the higher quartz glass shell of a small fusing point, and
Since quartz glass cannot obstruct the ultraviolet and infrared ray that are sent during halogen lamp work, therefore halogen lamp is as biomedical instrument
Usually it is required for configuring corresponding optical filtering during light source works.
Laser is the electric light source to be shone using excited state particle under stimulated radiation effect, since the T.H. in the nineteen sixty U.S.
Since ruby laser is made in Mei Man, the kinds of all kinds of laser light sources is up to hundreds of, and output wavelength scope is from short wavelength UV
Until far infrared.Although monochromaticjty, directionality, coherence are good, brightness is high, and cost and maintenance cost are extremely expensive, and needs
Strictly to control its security.
For flow cytometer in the prior art and DPCR instrument excitation source cannot take into account light conversion efficiency, durability degree,
The problem of cost and spectral region, there has been no effective solution at present.
The content of the invention
For flow cytometer in correlation technique and DPCR instrument excitation source cannot take into account light conversion efficiency, durability degree,
The problem of cost and spectral region, it is an object of the invention to propose that a kind of flow cytometer shines with DPCR instrument addressables LED
Array is penetrated, the high level of light conversion efficiency, durability degree, cost and spectral region can be taken into account, is reached in relatively low cost level
To the requirement of the working index of flow cytometer and DPCR instrument excitation sources.
Based on above-mentioned purpose, technical solution provided by the invention is as follows：
According to an aspect of the invention, there is provided a kind of flow cytometer and DPCR instrument addressable LED illumination array,
Including：
Illumination unit, illumination unit is placed in flow cytometer and in DPCR instrument, illumination unit is in positive flow cytometer
Flow chamber outlet port at or the filter of DPCR instrument at be fixedly installed, illumination unit be used at the flow chamber outlet port of flow cytometer,
Or exciting light is sent at the filter of DPCR instrument, excitation tested cell, particle or nucleic acid molecules send fluorescence；
Bonding units, bonding units are electrically connected to illumination unit and driving unit, and bonding units are by using bonding method
Establish and be electrically connected between illumination unit and driving unit；
Driving unit, driving unit are electrically connected to bonding units and control unit, and driving unit is used in control unit
Control under scanned according to clock frequency and drive illumination unit to shine；
Control unit, control unit are electrically connected to driving unit, and control unit is used to store and perform set in advance
The control program of illumination unit, control unit, which performs control program, makes requirement of the illumination unit according to control program set in advance
Send exciting light.
Wherein, illumination unit includes：
Supporting substrate, supporting substrate are a transparent thin board, flow chamber outlet port or DPCR of the supporting substrate towards flow cytometer
The filter of instrument is set, and supporting substrate is used for the position for fixing LED array；
LED array, LED array include multiple LED units, and multiple LED units are fixed on supporting substrate towards fluidic cell
The side of the flow chamber outlet port of instrument or the filter of DPCR instrument, multiple LED units are arranged in matrix in supporting substrate, irradiation
Unit sends exciting light for sending multiple LED units that exciting light is illumination unit, and each LED unit the independently-controlled can be sent out
Light；
A plurality of conducting wire, a plurality of arrangement of conductors include a plurality of row conductor and a plurality of row in the both sides of supporting substrate, a plurality of conducting wire
Conducting wire, a plurality of row conductor are distributed in the side of supporting substrate, and a plurality of column wire is distributed in the opposite side of supporting substrate；
Multiple through holes, multiple through holes are arranged on supporting substrate, and the quantity of multiple through holes is equal to the number of multiple LED units
Amount, each through hole is corresponding with a LED unit, and each through hole has a LED unit to extend there through, and multiple through holes are used for
Multiple LED units are made to establish electric connection between a plurality of row conductor and a plurality of column wire.
And：
The sum of line number that the quantity of a plurality of conducting wire is arranged in matrix for multiple LED units and columns, wherein, a plurality of row
The quantity of conducting wire is the columns that is arranged in matrix of multiple LED units, and the quantity of a plurality of column wire is multiple LED units with square
The line number of formation formula arrangement；
Each LED unit in LED array includes a light emitting diode, and each LED unit in LED array has two
A pin, wherein, a plurality of row of supporting substrate side is electrically connected to by through hole with the pin that light emitting diode cathode is connected
Conducting wire, a plurality of column wire of supporting substrate opposite side is electrically connected to the pin that light emitting diode anode is connected by through hole.
Also, the LED unit of LED array shines as with one kind in ultraviolet light, infrared light, X-ray, visible ray or more
Kind；The constituent material of supporting substrate is quartz, the one or more in monocrystalline silicon piece, plastics, metal.
Meanwhile a plurality of conducting wire is a plurality of metal or the conductive material conducting wire of 0~0.5mm thickness；Through hole is dry ecthing
Or wet etching hole.
Meanwhile bonding units are a pcb board, include array interface and high-purity bonding line on pcb board, array on pcb board
The quantity of interface and high-purity bonding line is equal to the quantity of a plurality of conducting wire of supporting substrate；By using bonding method illumination unit with
Establish and be electrically connected between driving unit, the array interface being electrically connected to for high-purity bonding line one end on pcb board, the other end
It is electrically connected to corresponding every conducting wire.
Also, illumination unit is further included with the connection of bonding units and is fixedly connected, the supporting substrate of illumination unit is backwards to stream
Fixed at the flow chamber outlet port of formula cell instrument or at the filter of DPCR instrument between the pcb board of bonding units by adhesion.
Meanwhile driving unit includes constant-current LED driver and latches Source drive with serial input, wherein, constant-current LED driving
Device is electrically connected to the negative pin of each LED unit, and constant-current LED driver is used for according to the clock frequency of control unit to sweep
The mode retouched latches the cathode that Source drive is electrically connected to each LED unit to each LED unit constant current output, serial input
Pin, serial input latch Source drive and carry out data latch in a manner of serial input-parallel exports.
Also, constant-current LED driver is a MAX6971 chip, and it is two MIC5891 that serial input, which latches Source drive,
Chip, wherein, two MIC5891 chips are electrically connected to support by the array interface on pcb board and high-purity bonding line
The a plurality of row conductor of substrate side, a MAX6971 chip are electrically connected by the array interface on pcb board and high-purity bonding line
It is connected to a plurality of column wire of supporting substrate opposite side.
Also, control unit is FPGA plates, FPGA plates are used to store and perform control program set in advance, and FPGA plates are held
Row control one MAX6971 chip of programme-control and two MIC5891 chip drives LED arrays are according to control journey set in advance
The requirement of sequence sends exciting light；Multiple LED units have identical or a variety of different wave lengths, identical or a variety of different capacities shine
Diode, control program set in advance are used to control whether each LED unit in LED array to shine and luminous intensity size.
From the above it can be seen that technical solution provided by the invention is by using illumination unit replacing halogen lamp and swashs
Light irradiation excitation tested cell, particle or nucleic acid molecules, improve light conversion efficiency relative to halogen lamp and reduce energy consumption, phase
Cost is considerably reduced for laser and improves security, has accomplished to take into account light conversion efficiency, durability degree, cost and light
The high level of spectral limit, the working index that flow cytometer and DPCR instrument excitation sources are reached in relatively low cost level will
Ask.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to institute in embodiment
Attached drawing to be used is needed to be briefly described, it should be apparent that, drawings in the following description are only some implementations of the present invention
Example, for those of ordinary skill in the art, without creative efforts, can also obtain according to these attached drawings
Obtain other attached drawings.
Fig. 1 is the part-structure figure of flow cytometer in the prior art；
Fig. 2 is the part-structure figure of DPCR instrument in the prior art；
Fig. 3 is the structural frames of the flow cytometer according to the embodiment of the present invention and DPCR instrument addressable LED illumination arrays
Figure；
Fig. 4 is single with being irradiated in DPCR instrument addressable LED illumination arrays according to the flow cytometer of the embodiment of the present invention
The structure diagram of member；
Fig. 5 be according to the flow cytometer of the embodiment of the present invention with it is single led in DPCR instrument addressable LED illumination arrays
The structure principle chart of unit；
Fig. 6 is the flow cytometer according to the embodiment of the present invention and a LED in DPCR instrument addressable LED illumination arrays
The structure chart of array；
Fig. 7 is single with being bonded in addressable LED illumination array with DPCR instrument for the flow cytometer according to the embodiment of the present invention
The structure chart of first pcb board.
Embodiment
For the object, technical solutions and advantages of the present invention are more clearly understood, below in conjunction with the embodiment of the present invention
Attached drawing, the technical solution in the embodiment of the present invention is further carried out it is clear, complete, describe in detail, it is clear that it is described
Embodiment is only part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this area
Those of ordinary skill's all other embodiments obtained, belong to the scope of protection of the invention.
At present, made mostly using halogen lamp, laser, LED (light emitting diode) in many biomedical instrument courses of work
Sample is excited for light source, so as to achieve the purpose that tracing detection cell or particle.Existing Flow Cytometry and DPCR (numeral
PCR) technology is just respectively generally using laser beam and halogen lamp as excitation source.
Fig. 1 is illustrated that the part-structure of flow cytometer in the prior art.The optical system of flow cytometer is by one
Light source and a detector are formed, and flow indoor sample cell or particle in a manner of single successively at a high speed by flow chamber sluice gate, and
Irradiated by laser beam, gather cell or the particle various signals caused by illumination, signal is handled, and to each parameter into
Row association analysis.These parameters are by cell or particle by scattering incident laser during flow chamber sluice gate and to send corresponding fluorescence true
Fixed, scattering light and fluorescence is collected by the light collecting device suitably positioned, and scattering light and fluorescence are sent to corresponding detection
Device detects, and the parameter that detector measures includes the relative size of particle, relative granularity, internal complexity and relative luminous intensity.
Digital pcr technology be it is a kind of based on single-molecule PCR method come the nucleic acid molecules absolute quantitation technology counted, phase
Than being very different in quantitative PCR technique.Quantitative PCR is to measure nucleic acid amount by standard curve or reference gene, and digital
PCR then can directly count the number of DNA molecular, be the absolute quantitation to initial sample.It is therefore particularly suitable for relying on Ct values
The application field that cannot be differentiated very well：Copy number variation, abrupt climatic change, (such as allele is uneven for gene relative expression research
Expression), the verification of two generation sequencing results, micro-RNA expression analysis, unicellular gene expression analysis etc..Fig. 2 is to be illustrated that technology
The part-structure of middle DPCR instrument.Whole DPCR instrument carries out in the thermal cycler, and incident light source forms specific wavelength through filter
Light beam irradiating sample plate on sample, sample scattered illumination light simultaneously sends corresponding fluorescence, scatters light and fluorescence passes through one
Filter is detected via detector, then through a series of filters, grating processing remove interference and by optical signal through opto-electronic conversion and
Computer is inputted after amplification, and is handled by software analysis.
Flow cytometer uses halogen lamp to use laser as excitation source as excitation source, DPCR instrument.
Halogen lamp is a kind of typical thermal light source, and principle is the injection halogen gas such as iodine or bromine in light bulb, at high temperature,
The tungsten filament of distillation carries out chemical action with halogen, and tungsten after cooling can be set on tungsten filament again, form the circulation of balance.Halogen
Although lamp is wide as excitation source spectral region, brightness is high, and electro-optical efficiency is low, and (generally 30%, remainder is with warm
Energy form is scattered and disappeared), energy consumption.Halogen lamp is made of tungsten filament, is enclosed in the higher quartz glass shell of a small fusing point, and
Since quartz glass cannot obstruct the ultraviolet and infrared ray that are sent during halogen lamp work, therefore halogen lamp is as biomedical instrument
Usually it is required for configuring corresponding optical filtering during light source works.
Laser is the electric light source to be shone using excited state particle under stimulated radiation effect, since the T.H. in the nineteen sixty U.S.
Since ruby laser is made in Mei Man, the kinds of all kinds of laser light sources is up to hundreds of, and output wavelength scope is from short wavelength UV
Until far infrared.Although monochromaticjty, directionality, coherence are good, brightness is high, and cost and maintenance cost are extremely expensive, and needs
Strictly to control its security.
Nowadays, researcher is not only directed to improving the function of biomedical instrument, they also focus on limited at one
Price and have the function of to develop a small integrated, automated system on the premise of equal use.When an integrated level compared with
When high Medical Instruments is flourishing, cost and the volume of photoelectric conversion device become deciding factor.
According to an embodiment of the invention, there is provided a kind of flow cytometer and DPCR instrument addressable LED illumination array.
As shown in figure 3, the flow cytometer provided according to embodiments of the present invention and DPCR instrument addressable LED illumination arrays
Including：
Illumination unit 1, illumination unit 1 is placed in flow cytometer and in DPCR instrument, illumination unit 1 is in positive flow cytometric
It is fixedly installed at the flow chamber outlet port of instrument or at the filter of DPCR instrument, illumination unit 1 is used for the flow chamber outlet port to flow cytometer
Exciting light is sent at the filter of place or DPCR instrument, excitation tested cell, particle or nucleic acid molecules send fluorescence；
Bonding units 2, bonding units 2 are electrically connected to illumination unit 1 and driving unit 3, and bonding units 2 are by using key
Legal established between illumination unit 1 and driving unit 3 is electrically connected；
Driving unit 3, driving unit 3 are electrically connected to bonding units 2 and control unit 4, and driving unit 3 is used to control
Scanned under the control of unit 4 according to clock frequency and drive illumination unit 1 to shine；
Control unit 4, control unit 4 are electrically connected to driving unit 3, and control unit 4 is used to store and perform to set in advance
The control program of fixed illumination unit 1, control unit 4, which performs control program, makes illumination unit 1 according to control journey set in advance
The requirement of sequence sends exciting light.
As shown in figure 4, illumination unit 1 includes：
Supporting substrate 11, supporting substrate 11 are a transparent thin board, supporting substrate 11 towards flow cytometer flow chamber outlet port,
Or the filter of DPCR instrument is set, supporting substrate 11 is used for the position for fixing LED array 12；
LED array 12, LED array 12 include multiple LED units, and multiple LED units are fixed on supporting substrate 11 towards stream
The side of the flow chamber outlet port of formula cell instrument or the filter of DPCR instrument, multiple LED units are arranged in the matrix form in supporting substrate 11
Row, illumination unit 1 send exciting light, each LED unit for sending multiple LED units that exciting light is illumination unit 1
It is the independently-controlled to shine；
A plurality of conducting wire 13, a plurality of conducting wire 13 are distributed in the both sides of supporting substrate 11, and a plurality of conducting wire 13 includes a plurality of row conductor
With a plurality of column wire, a plurality of row conductor is distributed in the side of supporting substrate 11, and a plurality of column wire is distributed in supporting substrate 11
Opposite side；
Multiple through holes 14, multiple through holes 14 are arranged on supporting substrate 11, and the quantity of multiple through holes 14 is equal to multiple LED
The quantity of unit, each through hole is corresponding with a LED unit, and each through hole has a LED unit to extend there through, multiple
Through hole 14 is used to make multiple LED units establish electric connection between a plurality of row conductor and a plurality of column wire.
Also, as shown in Figure 4：
The sum of line number that the quantity of a plurality of conducting wire 13 is arranged in matrix for multiple LED units and columns, wherein, it is a plurality of
The quantity of row conductor is the columns that is arranged in matrix of multiple LED units, the quantity of a plurality of column wire for multiple LED units with
The line number of matrix form arrangement；
Each LED unit in LED array 12 includes a light emitting diode, and each LED unit in LED array 12 is equal
There are two pins, wherein, 11 side of supporting substrate is electrically connected to by through hole with the pin that light emitting diode cathode is connected
A plurality of row conductor, a plurality of of 11 opposite side of supporting substrate is electrically connected to the pin that light emitting diode anode is connected by through hole
Column wire.
The circuit structure principle of single led unit is as shown in Figure 5.In Figure 5, the cathode of light emitting diode connects power supply, bears
Pole series connection one current-limiting resistance R ground connection.Wherein, current-limiting resistance R is optional resistance, is judged whether according to the situation of extraneous size of current
Current-limiting resistance R and resistance value size are needed, not indispensability；In the present embodiment, LED array 12 is supplied using constant-current source driver
Electricity, light emitting diode not series limiting resistor R.When using current-limiting resistance R, the difference according to VCC cathode voltages (is typically
3.3V or 5V) and the type kind of light emitting diode it is different, the value of current-limiting resistance R is also different.
Multiple LED units can be arranged in matrix into matrix as shown in Figure 6.In figure 6,64 LED unit quilts
The matrix of 8*8 is lined up, the cathode with each LED unit of a line links together, and the anode of each LED unit of same row connects
It is connected together, a positive wire for being in the row LED unit is drawn per a line, each row extraction is in one of the column unit
Cathode conductor, the LED array 12 of a N*M contain N+M root conducting wires, are in figure 6 16.This addressable LED unit row
Row scheme can embody the advantage of less occupancy Hardware I/O mouthfuls in complicated multi-channel transmission channel.
Also, the LED unit of LED array 12 shine as with one kind in ultraviolet light, infrared light, X-ray, visible ray or
It is a variety of；The constituent material of supporting substrate 11 is quartz, the one or more in monocrystalline silicon piece, plastics, metal.
Also, a plurality of conducting wire 13 is a plurality of metal or the conductive material conducting wire of 0~0.5mm thickness；Through hole is dry corrosion
Quarter or wet etching hole.One N*MLED array 12 is by N roots metal or conductive material row conductor and M roots metal or conduction material
Material column wire processed connects the cathode and anode for each LED unit put in the matrix form on supporting substrate 11 respectively.
In addition, LED array 12 can realize the two-sided adhesion on one piece of supporting substrate 11 and work, branch support group can be reached
11 area of plate utilizes maximization.
As shown in Fig. 4 and Fig. 7, bonding units 2 are a pcb board, include array interface 21 and high-purity bonding line on pcb board
22, the quantity of array interface 21 and high-purity bonding line 22 is equal to the quantity of a plurality of conducting wire 13 on supporting substrate 11 on pcb board；It is logical
Cross and electric connection is established between illumination unit 1 and driving unit 3 using bonding method, electrically connect for 22 one end of high-purity bonding line
The array interface 21 being connected on pcb board, the other end are electrically connected to corresponding every conducting wire.
Also, illumination unit 1 is further included with the connection of bonding units 2 and is fixedly connected, the supporting substrate 11 of illumination unit 1 is carried on the back
Consolidated to the flow chamber outlet port of flow cytometer or at the filter of DPCR instrument between the pcb board of bonding units 2 by adhesion
It is fixed.Leaving blank with 5mm*5mm on the pcb board shown in Fig. 7, this 5mm*5mm is the ruler of the supporting substrate 11 shown in Fig. 4
It is very little.
Meanwhile driving unit 3 includes constant-current LED driver and latches Source drive with serial input, wherein, constant-current LED drives
Dynamic device is electrically connected to the negative pin of each LED unit, and constant-current LED driver is used for the clock frequency according to control unit 4
Latch Source drive to each LED unit constant current output, serial input in a manner of scanning and be electrically connected to each LED unit
Cathode pin, serial input latch Source drive and carry out data latch in a manner of serial input-parallel exports.
Also, constant-current LED driver is a MAX6971 chip, and it is two MIC5891 that serial input, which latches Source drive,
Chip, wherein, two MIC5891 chips are electrically connected to by the array interface 21 on pcb board with high-purity bonding line 22
The a plurality of row conductor of 11 side of supporting substrate, a MAX6971 chip are bonded by the array interface 21 on pcb board with high-purity
Line 22 is electrically connected to a plurality of column wire of 11 opposite side of supporting substrate.
The anode of one MAX6971 chips connection LED array 12 coordinates two MIC5891 chips to connect LED array 12
Cathode drives LED array 12 by the method scanned at the same time to anode and cathode two-way, once scan frequency is sufficiently high, due to
The persistence of vision effect of human eye, the flicker human eye of the LE unit pieces on supporting substrate 11 will be invisible.
MIC5891 is one and the 8 Bits Serials input with high voltage, high current is made of eight cmos data latch cicuits
Source drive is latched, under+5V logic powers, which is usually operated under the clock frequency of 5MHz, works as input serial data
During more than 8, multi-disc MIC5891 chips can be cascaded to meet needs.The present invention is connected using the method for two panels MIC5891 cascades
Connect for up to 16 road cathode common leads of LED array 12, the characteristics of using chip data serial input, the parallel output, energy
Hardware resource is greatly saved, takes I/O mouthfuls at least.
MAX6971 is a serial input, 16 port 36V constant current output constant-current LED drivers, be usually operated at 3V~
Under 5.5V power supplys.When input serial data is more than 16, multi-disc MAX6971 chips can be cascaded to meet needs.The present invention adopts
The anode common lead that LED array 12 is for up to 16 tunnels is connected with a piece of MAX6971, when work only needs the chip special pin
As soon as (327.3 ohm~5 kilohms) of the outer connecting resistance of connection to, side that can be according to the clock frequency that FPGA plates are given to scan
Formula controls 16 road port constant current outputs.
12 driving chip of LED array is also not limited to MIC5891 and MAX5891, can be other with similar functions
The combination of chip.If in addition, 12 scale is smaller of required LED array, main control chip can use 51 microcontrollers or other species
8-16 positions microcontroller be used as the drive control chip of LED array 12.
Also, control unit 4 is FPGA plates, FPGA plates are used to store and perform control program set in advance, FPGA plates
Control one MAX6971 chip of programme-control and two MIC5891 chip drives LED arrays 12 are performed according to control set in advance
The requirement of processing procedure sequence sends exciting light.
FPGA, that is, field programmable gate array, is occurred as a kind of semi-custom circuit in application-specific integrated circuit field
, not only solved the deficiency of custom circuit, but also the shortcomings that original programming device gate circuit number amount is limited is overcome, can be with hard
Part description language completes circuit design, by simple comp comprehensive layout, is quickly burned onto on FPGA and is tested.The present invention is logical
Verilog HDL programmings are crossed, the Nexys4 development boards of company of match Sentos production is burned onto, then controls a piece of MAX6971 and two
Piece MIC5891 drivings LED array 12 works, and the design cycle is shorter.The burning program that we are responsible for finishing using upper PC machine
Into FPGA plates, FPGA plates perform the program of burning, and export signal by I/O interface controls a piece of MAX6971 chips and two at the same time
Piece MIC5891 chips；One MAX6971 chip is controlled by FPGA plates, is responsible for 12 cathode common lead of completion sample LED array and is swept
Retouch function and provide constant current driving for LED array 12；Two MIC5891 chips are controlled by FPGA plates, are responsible for completing LED gusts of sample
12 anode common lead scan function of row.
Meanwhile drive the work of LED array 12 to be not limited to fixed program, it can also realize man-machine friendship in certain method
Mutually, it is capable of the luminous intensity of some LED chips of the light on and off for changing some LED units in LED array 12 or change middle at any time.
Also, multiple LED units have identical or a variety of different wave lengths, identical or a variety of different capacities light-emitting diodes
Pipe, control program set in advance are used to control whether each LED unit in LED array 12 to shine and luminous intensity size.LED gusts
Row 12 are not limited to the combination of single LED unit or multi-wavelength, the combination of multiple power LED chip.
In the present embodiment, we use 11 blue LED arrays 12 of 5mm*5mm supporting substrates and a 5mm*5mm
11 colour mixture of supporting substrate (RGB) LED array 12, wherein, LED unit all selects the chip that specification is 230um*305um.
User can configure the parameter of LED unit according to different application requirements.LED array 12 based on FPGA drivings is as biology
The light source sample 5mm*5mmLED arrays 12 of Medical Instruments, clock is 2.5MHz when operating, power supply 5V, and sweep speed is
During 10.5kHz, the power for measuring 5mm*5mmLED arrays 12 is 67uW/ square millimeters.The light conversion ratio of the array is about 88%,
Available for the various kinds of cell including flow cytometer and DPCR instrument and genetic engineering instrument, quantity, the color of LED unit
Size of (wavelength), power and array etc. can be selected flexibly.
In addition, LED array 12 utilizes its small, advantage easily moved, lighting source can be used as in medical diagnosis,
Such as oral cavity illumination；Consume energy low using LED unit and LED modules with different colors artificial selection can be combined in supporting substrate 11
Advantage, the specific aim supplementary lighting sources that can be used as in the production of current agricultural greenhouse, promotes the photosynthesis of crops；LED
LED unit on array 12 changes infrared LED units into, and it is red to may be used as camera in the light source and security device of infrared detecting set
Outer illumination.
The present invention changes the LED array of efficient (reaching as high as 90%) as in biomedical instrument equipment using electric light dress
Cold light source, due to form LED array LED unit glow color the spectral region of light source can both have been expanded with unrestricted choice,
Ultraviolet and infrared radiation problem are overcome again.
The shortcomings that for laser as light source cost and high maintenance cost, the LED unit about people that the present invention uses
5 yuan/of coin, 75 LED chips in sample LED array need 375 yuan or so of RMB, certainly, the batch production of LED chip
It can make it that cost is lower.In addition, LED array can be dominated manually as the combination of monochromatic or multi-colored led chip, this can overcome monochrome
Deficiency of the property laser as light source.
In conclusion by means of the above-mentioned technical proposal of the present invention, by using illumination unit replacing halogen lamp and laser
Irradiation excitation tested cell, particle or nucleic acid molecules, improve light conversion efficiency relative to halogen lamp and reduce energy consumption, its
In, illumination unit is to apply light emitting diode matrix based on the detection of supporting substrate addressable, it is possible to achieve in grade branch support group
A large amount of micro- LED chips are configured on plate, which is controlled and driven by FPGA plates, and light intensity output power is driven by constant-current LED
Chip coordinates scanning line method to realize；Present design considerably reduces cost relative to laser and improves security,
Accomplish to take into account the high level of light conversion efficiency, durability degree, cost and spectral region, and brightness can control, it is small, it is portable
Band, reaches the working index requirement of flow cytometer and DPCR instrument excitation sources in relatively low cost level.
Those of ordinary skills in the art should understand that：The foregoing is merely the specific embodiment of the present invention, and
The limitation present invention is not used in, within the spirit and principles of the invention, any modification, equivalent substitution, improvement and etc. done,
It should be included within protection scope of the present invention.
Claims (9)
1. a kind of flow cytometer and DPCR instrument addressable LED illumination array, it is characterised in that including：
Illumination unit, the illumination unit is placed in flow cytometer and in DPCR instrument, the illumination unit is thin in positive convection type
It is fixedly installed at the flow chamber outlet port of born of the same parents' instrument or at the filter of DPCR instrument, the illumination unit is used for the stream to flow cytometer
Exciting light is sent at the filter of room exit or DPCR instrument, excitation tested cell, particle or nucleic acid molecules send fluorescence；
Bonding units, the bonding units are electrically connected to the illumination unit and driving unit, and the bonding units are by making
Electric connection is established between the illumination unit and driving unit with bonding method；
Driving unit, the driving unit are electrically connected to the bonding units and control unit, and the driving unit is used for
Scanned under the control of control unit according to clock frequency and drive the illumination unit to shine；
Control unit, described control unit are electrically connected to the driving unit, and described control unit is used to store and perform pre-
The control program of the illumination unit first set, described control unit, which performs control program, makes the illumination unit according to advance
The requirement of the control program of setting sends exciting light；
The illumination unit includes：
Supporting substrate, the supporting substrate are the substrate of certain material, and the flow chamber of the supporting substrate towards flow cytometer goes out
The filter of mouth or DPCR instrument is set, and the supporting substrate is used for the position for fixing LED array；
LED array, the LED array include multiple LED units, the multiple LED unit be fixed on the supporting substrate towards
The side of the flow chamber outlet port of flow cytometer or the filter of DPCR instrument, the multiple LED unit is in the supporting substrate with square
Formation formula arranges, and the illumination unit sends exciting light for sending multiple LED units that exciting light is the illumination unit, institute
Each LED unit is stated the independently-controlled can to shine；
A plurality of conducting wire, a plurality of arrangement of conductors include a plurality of row conductor in the both sides of the supporting substrate, a plurality of conducting wire
With a plurality of column wire, a plurality of row conductor is distributed in the side of the supporting substrate, and a plurality of column wire is distributed in
The opposite side of the supporting substrate；
Multiple through holes, the multiple through hole are arranged on the supporting substrate, and the quantity of the multiple through hole is equal to described more
The quantity of a LED unit, each through hole is corresponding with a LED unit, and each through hole has a LED unit
Extend there through, the multiple through hole is used to make the multiple LED unit in a plurality of row conductor and a plurality of column wire
Between establish be electrically connected.
2. a kind of flow cytometer according to claim 1 and DPCR instrument addressable LED illumination array, its feature exist
In, including：
The sum of line number that the quantity of a plurality of conducting wire is arranged in matrix for the multiple LED unit and columns, wherein, institute
State the columns that the quantity of a plurality of row conductor is arranged in matrix for the multiple LED unit, the quantity of a plurality of column wire
The line number being arranged in matrix for the multiple LED unit；
Each LED unit in the LED array includes a light emitting diode, and each LED unit in the LED array is equal
There are two pins, wherein, the supporting substrate is electrically connected to by the through hole with the pin that light emitting diode cathode is connected
The a plurality of row conductor of side, the support is electrically connected to the pin that light emitting diode anode is connected by the through hole
The a plurality of column wire of substrate opposite side.
3. a kind of flow cytometer according to claim 2 and DPCR instrument addressable LED illumination array, its feature exist
In the LED unit of the LED array shines as at least one of：Ultraviolet light, infrared light, X-ray, visible ray；The branch
The constituent material of support group plate is at least one of：Including quartz, monocrystalline silicon piece, plastics, metal.
4. a kind of flow cytometer according to claim 2 and DPCR instrument addressable LED illumination array, its feature exist
In a plurality of conducting wire is a plurality of metal or the conductive material conducting wire of 0~0.5mm thickness；The through hole be dry ecthing or
Wet etching hole.
5. a kind of flow cytometer according to claim 2 and DPCR instrument addressable LED illumination array, its feature exist
In the bonding units are a pcb board, include array interface and high-purity bonding line on the pcb board, the pcb board is gone into battle
The quantity of row interface and high-purity bonding line is equal to the quantity of a plurality of conducting wire of the supporting substrate；It is described to be existed by using bonding method
Establish and be electrically connected between the illumination unit and driving unit, be electrically connected to for described high-purity bonding line one end described
Array interface on pcb board, the other end are electrically connected to the corresponding every conducting wire.
6. a kind of flow cytometer according to claim 5 and DPCR instrument addressable LED illumination array, its feature exist
In the illumination unit is further included with the connection of the bonding units and is fixedly connected, and the supporting substrate of the illumination unit is backwards
Consolidated at the flow chamber outlet port of flow cytometer or at the filter of DPCR instrument between the pcb board of the bonding units by adhesion
It is fixed.
7. a kind of flow cytometer according to claim 5 and DPCR instrument addressable LED illumination array, its feature exist
In, the driving unit includes constant-current LED driver and latches Source drive with serial input, wherein, the constant-current LED driver
The negative pin of each LED unit is electrically connected to, the constant-current LED driver is used for the clock frequency according to control unit
Rate latches Source drive and is electrically connected to institute in a manner of scanning to each LED unit constant current output, the serial input
The cathode pin of each LED unit is stated, the serial input latches Source drive and carried out in a manner of serial input-parallel exports
Data latch.
8. a kind of flow cytometer according to claim 7 and DPCR instrument addressable LED illumination array, its feature exist
In the constant-current LED driver is a MAX6971 chip, and it is two MIC5891 cores that the serial input, which latches Source drive,
Piece, wherein, described two MIC5891 chips are electrically connected by the array interface on the pcb board and high-purity bonding line
To a plurality of row conductor of the supporting substrate side, one MAX6971 chips are connect by the array on the pcb board
Mouth is electrically connected to a plurality of column wire of the supporting substrate opposite side with high-purity bonding line.
9. a kind of flow cytometer according to claim 8 and DPCR instrument addressable LED illumination array, its feature exist
In described control unit is FPGA plates, and the FPGA plates are used to store and perform control program set in advance, the FPGA plates
The control one MAX6971 chips of programme-control are performed with LED array described in described two MIC5891 chip drives according to pre-
The requirement of the control program first set sends exciting light；The multiple LED unit have identical or a variety of different wave lengths, it is identical or
The light emitting diode of a variety of different capacities, the control program set in advance are used to control each LED described in the LED array
Whether unit shines and luminous intensity size.
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| CN109634043B (en) * | 2019-02-22 | 2020-08-28 | 中国科学院福建物质结构研究所 | Laser emission unit and laser projection light source |
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