CN105154572A - Specific 5'-terminal quantitative PCR (polymerase chain reaction) detection primers for transgenic rice PA110-15 line - Google Patents
Specific 5'-terminal quantitative PCR (polymerase chain reaction) detection primers for transgenic rice PA110-15 line Download PDFInfo
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- CN105154572A CN105154572A CN201510685106.4A CN201510685106A CN105154572A CN 105154572 A CN105154572 A CN 105154572A CN 201510685106 A CN201510685106 A CN 201510685106A CN 105154572 A CN105154572 A CN 105154572A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
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Abstract
Description
Reagent | Final concentration | Volume |
Sterilized water | 8μL | |
2 × TaqMan reaction buffer | 1× | 12.5μL |
10μmol/L PA110-5-qF | 0.4μmol/L | 1μL |
10μmol/L PA110-5-qR | 0.4μmol/L | 1μL |
10μmol/L PA110-5-P | 0.2μmol/L | 0.5μL |
DNA profiling | 2.0μL | |
Cumulative volume | 25.0μL |
Experimental group | a | b | a/b(%) | Theoretical content |
Experimental group 1 | 2527 | 3134 | 80.63% | 80% |
Experimental group 2 | 1466 | 2884 | 50.84% | 50% |
Experimental group 3 | 1206 | 2969 | 40.61% | 40% |
Experimental group 4 | 537 | 2794 | 19.21% | 20% |
Experimental group | a | b | a/b(%) | Theoretical content |
Experimental group 1 | 1209 | 3038 | 39.80% | 80% |
Experimental group 2 | 832 | 2904 | 28.65% | 50% |
Experimental group 3 | 546 | 3107 | 17.57% | 40% |
Experimental group 4 | 235 | 3022 | 7.78% | 20% |
Experimental group | a | b | a/b(%) | Theoretical content |
Experimental group 1 | 2001 | 3171 | 63.10% | 80% |
Experimental group 2 | 1119 | 3088 | 36.24% | 50% |
Experimental group 3 | 447 | 3101 | 14.41% | 40% |
Experimental group 4 | 308 | 2989 | 10.30% | 20% |
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CN201510685106.4A CN105154572A (en) | 2015-10-20 | 2015-10-20 | Specific 5'-terminal quantitative PCR (polymerase chain reaction) detection primers for transgenic rice PA110-15 line |
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CN201510685106.4A CN105154572A (en) | 2015-10-20 | 2015-10-20 | Specific 5'-terminal quantitative PCR (polymerase chain reaction) detection primers for transgenic rice PA110-15 line |
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CN201510685106.4A Pending CN105154572A (en) | 2015-10-20 | 2015-10-20 | Specific 5'-terminal quantitative PCR (polymerase chain reaction) detection primers for transgenic rice PA110-15 line |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105803092A (en) * | 2016-05-04 | 2016-07-27 | 黄埔出入境检验检疫局综合技术服务中心 | Transgenic alfalfa KK179-2 strain specific real-time fluorescence PCR detecting primer, probe, detecting kit and detecting method |
CN108374040A (en) * | 2018-04-04 | 2018-08-07 | 上海交通大学 | The LAMP detection method of transgenic paddy rice PA110-15 strain specificities |
CN112725423A (en) * | 2020-12-31 | 2021-04-30 | 镇江华大检测有限公司 | Detection method of transgenes in rice |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102453749A (en) * | 2010-10-18 | 2012-05-16 | 中国科学院上海生命科学研究院 | Method for detecting transgene component in food |
CN103966208A (en) * | 2014-03-06 | 2014-08-06 | 安徽省农业科学院水稻研究所 | Transgenic rice PA110-15 foreign insert flanking sequence and application |
-
2015
- 2015-10-20 CN CN201510685106.4A patent/CN105154572A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102453749A (en) * | 2010-10-18 | 2012-05-16 | 中国科学院上海生命科学研究院 | Method for detecting transgene component in food |
CN103966208A (en) * | 2014-03-06 | 2014-08-06 | 安徽省农业科学院水稻研究所 | Transgenic rice PA110-15 foreign insert flanking sequence and application |
Non-Patent Citations (2)
Title |
---|
夏邦顺 等: "《临床分子诊断学》", 31 July 2012 * |
王渭霞 等: "实时定量PCR检测转基因水稻科丰6号插入拷贝数和转基因含量", 《农业生物技术学报》 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105803092A (en) * | 2016-05-04 | 2016-07-27 | 黄埔出入境检验检疫局综合技术服务中心 | Transgenic alfalfa KK179-2 strain specific real-time fluorescence PCR detecting primer, probe, detecting kit and detecting method |
CN108374040A (en) * | 2018-04-04 | 2018-08-07 | 上海交通大学 | The LAMP detection method of transgenic paddy rice PA110-15 strain specificities |
CN112725423A (en) * | 2020-12-31 | 2021-04-30 | 镇江华大检测有限公司 | Detection method of transgenes in rice |
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Inventor after: Dong Mei Inventor after: Miao Chaohua Inventor after: Huang Weihong Inventor after: Li Liang Inventor after: Jin Wujun Inventor after: Wan Yusong Inventor after: Wang Di Inventor after: An Na Inventor after: Liu Fangfang Inventor after: Wang Yong Inventor after: Tu Jumin Inventor before: Dong Mei Inventor before: Li Liang Inventor before: Jin Wujun Inventor before: Wan Yusong Inventor before: Wang Di Inventor before: An Na Inventor before: Liu Fangfang Inventor before: Miao Chaohua Inventor before: Huang Weihong |
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