CN105152353A - Method for treating heavy metals in sewage by Gram bacteria - Google Patents
Method for treating heavy metals in sewage by Gram bacteria Download PDFInfo
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- CN105152353A CN105152353A CN201510304659.0A CN201510304659A CN105152353A CN 105152353 A CN105152353 A CN 105152353A CN 201510304659 A CN201510304659 A CN 201510304659A CN 105152353 A CN105152353 A CN 105152353A
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- gram
- heavy metal
- bacteria
- sewage
- nitrogen
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- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 53
- 238000000034 method Methods 0.000 title claims abstract description 38
- 239000010865 sewage Substances 0.000 title claims abstract description 26
- 241000894006 Bacteria Species 0.000 title claims abstract description 16
- 150000002500 ions Chemical class 0.000 claims abstract description 54
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 41
- 239000010881 fly ash Substances 0.000 claims abstract description 27
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 25
- 239000002131 composite material Substances 0.000 claims abstract description 20
- 238000001179 sorption measurement Methods 0.000 claims abstract description 19
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 14
- 239000007864 aqueous solution Substances 0.000 claims abstract description 12
- 230000004913 activation Effects 0.000 claims abstract description 4
- 238000006243 chemical reaction Methods 0.000 claims abstract description 4
- 239000008367 deionised water Substances 0.000 claims abstract description 4
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 4
- 238000002156 mixing Methods 0.000 claims abstract description 4
- 239000011259 mixed solution Substances 0.000 claims abstract description 3
- 239000012528 membrane Substances 0.000 claims description 27
- 239000007788 liquid Substances 0.000 claims description 14
- 230000001580 bacterial effect Effects 0.000 claims description 11
- 238000009413 insulation Methods 0.000 claims description 10
- 238000003756 stirring Methods 0.000 claims description 10
- 229920001817 Agar Polymers 0.000 claims description 9
- 239000008272 agar Substances 0.000 claims description 9
- 239000004927 clay Substances 0.000 claims description 8
- 239000012153 distilled water Substances 0.000 claims description 8
- 239000000919 ceramic Substances 0.000 claims description 7
- 239000002245 particle Substances 0.000 claims description 7
- 239000006228 supernatant Substances 0.000 claims description 7
- 241000190950 Rhodopseudomonas palustris Species 0.000 claims description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 6
- 229930006000 Sucrose Natural products 0.000 claims description 6
- 239000002994 raw material Substances 0.000 claims description 6
- 239000005720 sucrose Substances 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 5
- 238000001471 micro-filtration Methods 0.000 claims description 5
- 241000351920 Aspergillus nidulans Species 0.000 claims description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 4
- 239000003513 alkali Substances 0.000 claims description 4
- 239000011575 calcium Substances 0.000 claims description 4
- 239000010883 coal ash Substances 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 239000004698 Polyethylene Substances 0.000 claims description 3
- 230000000844 anti-bacterial effect Effects 0.000 claims description 3
- 229940041514 candida albicans extract Drugs 0.000 claims description 3
- 239000003153 chemical reaction reagent Substances 0.000 claims description 3
- 235000013312 flour Nutrition 0.000 claims description 3
- 239000012510 hollow fiber Substances 0.000 claims description 3
- 239000002054 inoculum Substances 0.000 claims description 3
- 238000003780 insertion Methods 0.000 claims description 3
- 230000037431 insertion Effects 0.000 claims description 3
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 claims description 3
- -1 polyethylene Polymers 0.000 claims description 3
- 229920000573 polyethylene Polymers 0.000 claims description 3
- 239000011148 porous material Substances 0.000 claims description 3
- 239000012138 yeast extract Substances 0.000 claims description 3
- 239000005995 Aluminium silicate Substances 0.000 claims description 2
- 240000003936 Plumbago auriculata Species 0.000 claims description 2
- 239000006004 Quartz sand Substances 0.000 claims description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 2
- 239000004902 Softening Agent Substances 0.000 claims description 2
- 229920002472 Starch Polymers 0.000 claims description 2
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 claims description 2
- 235000012211 aluminium silicate Nutrition 0.000 claims description 2
- 239000010425 asbestos Substances 0.000 claims description 2
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 claims description 2
- 239000011230 binding agent Substances 0.000 claims description 2
- 238000001354 calcination Methods 0.000 claims description 2
- 229910052791 calcium Inorganic materials 0.000 claims description 2
- 229910021540 colemanite Inorganic materials 0.000 claims description 2
- 238000000748 compression moulding Methods 0.000 claims description 2
- 239000010459 dolomite Substances 0.000 claims description 2
- 229910000514 dolomite Inorganic materials 0.000 claims description 2
- 238000001035 drying Methods 0.000 claims description 2
- 238000007688 edging Methods 0.000 claims description 2
- 230000005294 ferromagnetic effect Effects 0.000 claims description 2
- 239000000835 fiber Substances 0.000 claims description 2
- 239000010439 graphite Substances 0.000 claims description 2
- 239000012535 impurity Substances 0.000 claims description 2
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 claims description 2
- 230000005291 magnetic effect Effects 0.000 claims description 2
- 229910052625 palygorskite Inorganic materials 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- 239000000047 product Substances 0.000 claims description 2
- 229910052895 riebeckite Inorganic materials 0.000 claims description 2
- 239000011435 rock Substances 0.000 claims description 2
- 238000007493 shaping process Methods 0.000 claims description 2
- HBMJWWWQQXIZIP-UHFFFAOYSA-N silicon carbide Chemical compound [Si+]#[C-] HBMJWWWQQXIZIP-UHFFFAOYSA-N 0.000 claims description 2
- 229910010271 silicon carbide Inorganic materials 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 229910001220 stainless steel Inorganic materials 0.000 claims description 2
- 239000010935 stainless steel Substances 0.000 claims description 2
- 239000008107 starch Substances 0.000 claims description 2
- 235000019698 starch Nutrition 0.000 claims description 2
- 239000004575 stone Substances 0.000 claims description 2
- OGIDPMRJRNCKJF-UHFFFAOYSA-N titanium oxide Inorganic materials [Ti]=O OGIDPMRJRNCKJF-UHFFFAOYSA-N 0.000 claims description 2
- 239000011022 opal Substances 0.000 claims 1
- 239000000843 powder Substances 0.000 abstract description 2
- 238000005119 centrifugation Methods 0.000 abstract 1
- 230000007613 environmental effect Effects 0.000 abstract 1
- 230000008569 process Effects 0.000 description 17
- 238000010521 absorption reaction Methods 0.000 description 8
- 239000010949 copper Substances 0.000 description 8
- 244000005700 microbiome Species 0.000 description 7
- 238000000746 purification Methods 0.000 description 7
- 239000002351 wastewater Substances 0.000 description 7
- 239000010931 gold Substances 0.000 description 6
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 4
- 238000000862 absorption spectrum Methods 0.000 description 4
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 3
- 230000000536 complexating effect Effects 0.000 description 3
- 229910052802 copper Inorganic materials 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 229910021645 metal ion Inorganic materials 0.000 description 3
- 238000009825 accumulation Methods 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 229910021389 graphene Inorganic materials 0.000 description 2
- 229910052759 nickel Inorganic materials 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 239000011701 zinc Substances 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- MSFSPUZXLOGKHJ-UHFFFAOYSA-N Muraminsaeure Natural products OC(=O)C(C)OC1C(N)C(O)OC(CO)C1O MSFSPUZXLOGKHJ-UHFFFAOYSA-N 0.000 description 1
- 108010013639 Peptidoglycan Proteins 0.000 description 1
- 229910004298 SiO 2 Inorganic materials 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000000274 adsorptive effect Effects 0.000 description 1
- 125000003368 amide group Chemical group 0.000 description 1
- 229910052787 antimony Inorganic materials 0.000 description 1
- WATWJIUSRGPENY-UHFFFAOYSA-N antimony atom Chemical compound [Sb] WATWJIUSRGPENY-UHFFFAOYSA-N 0.000 description 1
- 229910052785 arsenic Inorganic materials 0.000 description 1
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 229910052793 cadmium Inorganic materials 0.000 description 1
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 1
- QXJJQWWVWRCVQT-UHFFFAOYSA-K calcium;sodium;phosphate Chemical compound [Na+].[Ca+2].[O-]P([O-])([O-])=O QXJJQWWVWRCVQT-UHFFFAOYSA-K 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 230000006567 cellular energy metabolism Effects 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003610 charcoal Substances 0.000 description 1
- 229910052804 chromium Inorganic materials 0.000 description 1
- 231100000749 chronicity Toxicity 0.000 description 1
- 239000008139 complexing agent Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 229910052745 lead Inorganic materials 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 150000001455 metallic ions Chemical class 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 150000007522 mineralic acids Chemical class 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- LFGREXWGYUGZLY-UHFFFAOYSA-N phosphoryl Chemical group [P]=O LFGREXWGYUGZLY-UHFFFAOYSA-N 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000010970 precious metal Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000002910 solid waste Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000002594 sorbent Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000003206 sterilizing agent Substances 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L sulfate group Chemical group S(=O)(=O)([O-])[O-] QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 229910052716 thallium Inorganic materials 0.000 description 1
- BKVIYDNLLOSFOA-UHFFFAOYSA-N thallium Chemical compound [Tl] BKVIYDNLLOSFOA-UHFFFAOYSA-N 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Landscapes
- Water Treatment By Sorption (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses a method for adsorbing and fixing heavy metals in sewage by Gram bacteria. The method comprises carrying out activation on a strain in a nitrogen-free medium inclined plane, inoculating a nitrogen medium with the strain, carrying out shake cultivation, inoculating a nitrogen-free ore powder medium with the strain, carrying out shake cultivation for several days to obtain a Gram bacterium-fly ash composite material, adding deionized water into the Gram bacterium-fly ash composite material to obtain an aqueous solution of the Gram bacterium-fly ash composite material, adding the aqueous solution of the Gram bacterium-fly ash composite material into heavy metal ion-containing sewage, carrying out full mixing to obtain a uniform mixed solution, carrying out standing for adsorption equilibrium and then carrying out centrifugation on the reaction system. The method is suitable for treating sewage containing a plurality of heavy metal ions, has the characteristics of simpleness, environmental friendliness and no pollution, realizes treatment on a plurality of heavy metal ions by one step and has a clearance rate of 90% or more.
Description
Technical field
The present invention relates to a kind for the treatment of process containing heavy metal ion sewage, particularly relate to a kind of method utilizing gram-bacteria to adsorb set heavy metal, belong to purification of water quality and field of environment protection.
Background technology
Along with developing rapidly of China's industrial or agricultural, the quantity discharged of heavy metal ion-containing waste water is in increasing trend year by year, and all kinds of heavy metal in waste water content is constantly increasing on the one hand, and heavy metal kind also becomes increasingly sophisticated simultaneously.Consider that heavy metal is to the chronicity of the harm that human body and environment cause and seriousness on the other hand, country is also more and more stricter to the harmless treatment standard of heavy metal-containing waste water.Therefore, how economy, efficiently removal heavy metal ions in wastewater have become current very urgent task.
Traditional heavy metal treatment process due to its processing efficiency lower, and costly, process problem is single, easily cause again the shortcomings such as secondary pollution just gradually eliminate by new treatment process.
For the waste water comprising complicated heavy metal ion, the integrated conduct method that multiple purification techniques combines comes into one's own just day by day.Traditional purification means comprise charcoal absorption, organism complexing etc., a kind of recently method utilizing graphene oxide to remove heavy metal ion in water, although it can realize the adsorption cleaning to some heavy metal ion, but there is adsorbable heavy metal ion kind, limited amount, after process, Graphene reclaims the shortcoming of inconvenience.
Summary of the invention
The object of the present invention is to provide a kind of method utilizing gram-bacteria to adsorb set heavy metal ions in sewage, it can realize the adsorption cleaning to the sewage containing several different heavy metal ion, and process efficient, quick, simple, pollution-free, thus overcome deficiency of the prior art.
For achieving the above object, present invention employs following technical scheme:
Utilize gram-bacteria to adsorb a method for set heavy metal ions in sewage, step comprises:
The gram-bacteria adopted selects Gram-negative bacteria; its cell walls of its cell walls forms primarily of peptidoglycan, protein and lipid; the main functional group that can combine with metal ion in these components comprises carboxyl, phosphoryl, hydroxyl, sulfate group, amino and amide group etc., and wherein the atom such as nitrogen, oxygen, sulphur can provide lone-pair electron and metallic ion coordination complexing.The bacterial strain specifically selected is A.nidulans bacterium or Rhodopseudomonas palustris (Rhodopseudomonaspalustris) or Spherical red antibacterial.
Bacterial classification has access to nitrogen substratum after nitrogen-free agar slant activation, at 28-30 DEG C, shaking culture 5d under 120r/min condition, this is cultivated bacterium liquid by 20% inoculum size access without in nitrogen breeze substratum, at 28-30 DEG C, shaking culture 5d under 120r/min condition, obtain gram-bacteria-fly ash composite material used.Add deionized water again, make the aqueous solution of the gram-bacteria-fly ash composite material of concentration 2-6g/L.
There is nitrogen substratum: sucrose 10.0g, yeast extract paste 0.3g, (NH
4)
2sO
40.5g, CaCO
30.5g, MgSO
47H
2o1g, K
2hPO
41.0g, l.0L, pH value is 7.0-7.5 to distilled water.
Nitrogen-free agar: sucrose 5.0g, CaCO
30.1g, MgSO
47H
2o0.5g, FeCl
36H
2o1mg, Na
2hPO
412H
2o5.0g, l.0L, pH value is 7.0-7.5 to distilled water.
Without nitrogen breeze substratum: the first level flour coal ash adding 15g/L in nitrogen-free agar.Chemical reagent used is analytical pure.
Then, the aqueous solution of this gram-bacteria-fly ash composite material is added in the sewage containing heavy metal ion, through fully, be uniformly mixed to form mixed solution, leave standstill and reach adsorption equilibrium in more than 72 hours.
Next, centrifugal treating is carried out to reaction system, rotating speed 5000r/min, time 15min.Get supernatant liquid after centrifugal, drain after qualified after testing; Get the turbid liquid of bottom, stand-by.
In the inventive solutions, described heavy metal ion is selected from Zn
2+, Cd
2+, Cu
2+, Cr
3+and Pb
2+in any one or a few, the concentration of described heavy metal ion is 10mg/L-100mg/L.
The method utilizing gram-bacteria to adsorb set heavy metal ions in sewage of the present invention's proposition is a kind of novel method being suitable for processing complicated effluent containing heavy metal ions.
Micro heavy ion is only needed, general≤0.1mg/L in microorganism normal growth.But in the environment that some heavy metal ion contents are high, also find that there is microorganism growth.Such as, in the earth (Cu content reaches 68000mg/L) and water (Cu content reaches 100mg/L) of the very high muskeg of some copper (cu) content, fungal growth is still had; In the mineralic acid containing arsenic, antimony, although their concentration substantially exceeds the toxigenous level of biology, also still existed by the microflora be made up of algae, fungi, protozoon and bacterium etc.When heavy metal and compound thereof use as sterilant or sterilizing agent, overdose often, while obtaining effectiveness, has also caused the resistance of microorganism to each metal ion species.The adsorbable metal ion of microorganism also in cell surface accumulation, even can reach more than 90% of dry cell weight.The absorption of microorganism can be divided into non-specific absorption, if certain micro-organisms is to the absorption of copper, cadmium, zinc, nickel; And specificity absorption, as certain micro-organisms depends on cellular energy metabolism, to the absorption of thallium and accumulation etc.
The composition of flyash and character flyash are the solid waste that Hazards in Power Plant is discharged.Its main component is SiO
2, A1
2o
3, CaO, Fe
2o
3deng, simultaneously also containing other material a small amount of.Flyash is made up of the particulate with different structure and form, and wherein great majority are glass spheres, and the particle diameter of single powder coal ash particle is about 5 ~ 300 μm, average geometric particle diameter 40 μm.Flyash has vesicular structure, and porosity is generally 60%-75%, and specific surface area is generally at 2500 ~ 5000cm
2/ g, has stronger adsorptive power.
In gram-bacteria-fly ash composite material, bacterium heavy metal ion has adsorption, this comprises the absorption of bacterium surface heavy metal ion, the flocculation of Extracellular polymers heavy metal ion, and the complexing action of exocellular polysaccharide and organic acidic material heavy metal ion.Except bacterium is to except the absorption of ion, flyash itself is also good sorbent material, and energy absorbed portion heavy metal ion, therefore both combinations make adsorption effect strengthen.Gram-bacteria-fly ash composite material to the adsorption rate of 5 kinds of ions all higher than bacterial adsorption, especially to Pb
2+adsorption rate reaches more than 97%.To Pb
2+adsorption effect is superior is because bacterium is to Pb
2+selective adsorption.This characteristic makes the present invention can targetedly for the treatment of containing Pb
2+waste water.
Waste water after gram-bacteria-fly ash composite material process is after centrifugal, and supernatant liquor as clear as crystal, this illustrates that the settleability of matrix material is better than single bacterial adsorption.Because the specific surface area of flyash is large, adsorption efficiency is high, is suitable for processing the sewage containing contents of many kinds of heavy metal ion.Compared with the sewage water treatment method of the single component such as gac, organic complexing agent of prior art, technical scheme of the present invention is except having the features such as simple, green non-pollution, also have and once can process contents of many kinds of heavy metal ion, process heavy metal ion kind is many, clearance 90% with first-class advantage; The present invention simultaneously can also effectively process some precious metals, as platinum, gold etc.And the gram-bacteria-flyash of Adsorption of Heavy Metal Ions can be used for producing the material of construction such as cement, ceramic tile, the free heavy metal ion of occurring in nature is solidified.
Take off the turbid liquid of layer, be placed in drainage trap, this drainage trap comprises the membrane module in a direct insertion groove, and this membrane module is made up of polyethylene hollow fiber microfiltration membrane, membrane pore size 0.1 μm, film silk internal diameter 0.27mm, film silk external diameter 0.42mm, membrane area 2m
2, an air bell is set below membrane module, plays water quality mixing effect.Membrane module has water pump pipe, and the water flowed out through going out water pump pipe directly discharges, and this water meets emission standard after testing.Described microfiltration membrane is produced by MIT.
The water ratio of the turbid liquid of the lower floor in drainage trap, lower than after 40%, takes out membrane module, is taken out by residue and mix with ceramic raw material, after shaping, fire ceramic tile.
Embodiment
Embodiment 1
Utilize gram-bacteria to adsorb a method for set heavy metal ions in sewage, step comprises:
The bacterial strain selected is A.nidulans bacterium.Bacterial classification has access to nitrogen substratum after nitrogen-free agar slant activation, at 28-30 DEG C, shaking culture 5d under 120r/min condition, this is cultivated bacterium liquid by 20% inoculum size access without in nitrogen breeze substratum, at 28-30 DEG C, shaking culture 5d under 120r/min condition, obtain gram-bacteria-fly ash composite material used.Add deionized water again, make the aqueous solution of the gram-bacteria-fly ash composite material of concentration 2-6g/L.
There is nitrogen substratum: sucrose 10.0g, yeast extract paste 0.3g, (NH
4)
2sO
40.5g, CaCO
30.5g, MgSO
47H
2o1g, K
2hPO
41.0g, l.0L, pH value is 7.0-7.5 to distilled water.
Nitrogen-free agar: sucrose 5.0g, CaCO
30.1g, MgSO
47H
2o0.5g, FeCl
36H
2o1mg, Na
2hPO
412H
2o5.0g, l.0L, pH value is 7.0-7.5 to distilled water.
Without nitrogen breeze substratum: the first level flour coal ash adding 15g/L in nitrogen-free agar.Chemical reagent used is analytical pure.
Then, what the aqueous solution of this gram-bacteria-fly ash composite material is joined 1L contains Fe
3+, Cu
2+, Cr
2+ionic concn be in the sewage of 20mg/L, after stirring at room temperature process 1h leave standstill 72h, reach adsorption equilibrium, realize dirty water purification.Centrifugal treating is carried out to reaction system, rotating speed 5000r/min, time 15min.Get supernatant liquid after centrifugal, detect each heavy metal ion content with atomic absorption spectrum, find that clearance can reach more than 99.5%.
Embodiment 2
The bacterial strain selected is Rhodopseudomonas palustris (Rhodopseudomonaspalustris).The aqueous solution of this gram-bacteria-fly ash composite material is joined 1L, containing Fe
3+, Ni
2+, Cu
2+each ionic concn is in the sewage of 50mg/L, leaves standstill 72h, reach adsorption equilibrium, realize dirty water purification after stirring at room temperature process 1h.The other the same as in Example 1.Get the supernatant liquor atomic absorption spectrum after process and detect each heavy metal ion content, find Fe
3+the clearance of ion is 98.7%, Ni
2+the clearance of ion is 99.3%, Cu
2+the clearance of ion is 97.4%.
Embodiment 3
The bacterial strain selected is Spherical red antibacterial.The aqueous solution of this gram-bacteria-fly ash composite material is joined 1L, containing Au
3+ionic concn is in the sewage of 50mg/L, leaves standstill 72h, reach adsorption equilibrium, realize dirty water purification after stirring at room temperature process 1h.The other the same as in Example 1.Get the supernatant liquor atomic absorption spectrum after process and detect Au
3+ion content, finds Au
3+ion remaval rate can reach 99%.
Embodiment 4
The bacterial strain selected is A.nidulans bacterium.The aqueous solution of this gram-bacteria-fly ash composite material is joined 1L, containing Au
3+, Pb
2+, Cr
2+each ionic concn is in the sewage of 100mg/L, leaves standstill 120h, reach adsorption equilibrium, realize dirty water purification after stirring at room temperature process 1h.The other the same as in Example 1.Get the supernatant liquor atomic absorption spectrum after process and detect each heavy metal ion content, find Au
3+the clearance of ion is 90.7%, Pb
2+the clearance of ion is 91.3%, Cr
2+the clearance of ion is 90.4%.
Embodiment 5
Take off the turbid liquid of layer, be placed in drainage trap, this drainage trap comprises the membrane module in a direct insertion groove, and this membrane module is made up of polyethylene hollow fiber microfiltration membrane, membrane pore size 0.1 μm, film silk internal diameter 0.27mm, film silk external diameter 0.42mm, membrane area 2m
2, an air bell is set below membrane module, plays water quality mixing effect.Membrane module has water pump pipe, and the water flowed out through going out water pump pipe meets emission standard.Described microfiltration membrane is produced by MIT.
The water ratio of the turbid liquid of the lower floor in drainage trap is lower than after 40%, take out membrane module, residue is taken out, then by weight, get this residue 1 and sandstone 3, dolomite 4, high-calcium fly ass 1.5, phosphatic rock 12, asbestos 8, colemanite 0.8, quartz sand 30, nanometer silicon carbide fiber 4, starch 15, six ring stone 15, nano titanium oxide 0.5, alkali treatment modifying clay 20, stand-by;
The preparation method of described alkali treatment modifying clay is as follows: 1:3:2 takes kaolin, wilkinite, palygorskite clay in mass ratio, adds water to solid content about 30% and mixes, being placed in strong magnetic field circumstance and removing Ferromagnetic Impurities; The Ca (OH) of additional described one of clay raw materials gross weight 0.5 times again
2, mix, put into plumbago crucible and heat 2 hours at the temperature of 110 DEG C and use stainless steel stirring rod to stir, then be washed with distilled water to neutrality, dry, 300 DEG C of calcining 30min, pulverize, cross 240 mesh sieves, stand-by.
Above-mentioned raw materials is mixed, adds softening agent and binding agent, stir, then make the particle that particle diameter is 2mm.Then send into forming machine compression moulding under 80MPa, then the adobe suppressed being delivered in dry kiln and being dried to water content is 1.5%;
By the adobe of drying with the ramp to 1350 DEG C of 15 DEG C/min, insulation 2h, then be cooled to 880 DEG C with the speed of 5 DEG C/min, insulation 2h, 510 DEG C are cooled to again with the speed of 4 DEG C/min, insulation 1h, then with the ramp to 1160 DEG C of 8 DEG C/min, insulation 2.5h, 680 DEG C are cooled to again with the speed of 5 DEG C/min, insulation 1.5h, naturally cools to normal temperature, through edging, packs and obtain ceramic tile finished product.
Should be understood that, the present invention can represent with other the specific form without prejudice to spirit of the present invention or principal character.Therefore, no matter from which point, the above embodiment of the present invention can only be thought explanation of the present invention and can not limit the present invention, claim of the present invention indicates scope of the present invention, any change in the spirit and implication of claim, all should think to comprise within the scope of the claims.
Claims (7)
1. utilize gram-bacteria to adsorb a method for set heavy metal ions in sewage, it is characterized in that, step comprises:
The gram-bacteria adopted selects Gram-negative bacteria; Bacterial classification has access to nitrogen substratum after nitrogen-free agar slant activation, at 28-30 DEG C, shaking culture 5d under 120r/min condition, this is cultivated bacterium liquid by 20% inoculum size access without in nitrogen breeze substratum, at 28-30 DEG C, shaking culture 5d under 120r/min condition, obtain gram-bacteria-fly ash composite material used.Add deionized water again, make the aqueous solution of the gram-bacteria-fly ash composite material of concentration 2-6g/L;
There is nitrogen substratum: sucrose 10.0g, yeast extract paste 0.3g, (NH
4)
2sO
40.5g, CaCO
30.5g, MgSO
47H
2o1g, K
2hPO
41.0g, l.0L, pH value is 7.0-7.5 to distilled water;
Nitrogen-free agar: sucrose 5.0g, CaCO
30.1g, MgSO
47H
2o0.5g, FeCl
36H
2o1mg, Na
2hPO
412H
2o5.0g, l.0L, pH value is 7.0-7.5 to distilled water;
Without nitrogen breeze substratum: the first level flour coal ash adding 15g/L in nitrogen-free agar.Chemical reagent used is analytical pure;
Then, the aqueous solution of this gram-bacteria-fly ash composite material is added in the sewage containing heavy metal ion, through fully, be uniformly mixed to form mixed solution, leave standstill and reach adsorption equilibrium in more than 72 hours;
Next, centrifugal treating is carried out to reaction system, rotating speed 5000r/min, time 15min.Get supernatant liquid after centrifugal, drain after qualified after testing.
2. utilize gram-bacteria to adsorb the method for set heavy metal ions in sewage as claimed in claim 1, it is characterized in that: described heavy metal ion is selected from Zn
2+, Cd
2+, Cu
2+, Cr
3+and Pb
2+in any one or a few, the concentration of described heavy metal ion can be 10mg/L-100mg/L.
3. utilize gram-bacteria to adsorb the method for set heavy metal ions in sewage as claimed in claim 1, it is characterized in that: preferred bacterial strain is A.nidulans bacterium or Rhodopseudomonas palustris (Rhodopseudomonaspalustris) or Spherical red antibacterial.
4. utilize gram-bacteria to adsorb the method for set heavy metal ions in sewage as claimed in claim 1, it is characterized in that: preferably the concentration of the aqueous solution of gram-bacteria-fly ash composite material is 2-6g/L.
5. utilize gram-bacteria to adsorb the method for set heavy metal ions in sewage as claimed in claim 1, it is characterized in that: get centrifugal after the turbid liquid of lower floor, be placed in drainage trap, this drainage trap comprises the membrane module in a direct insertion groove, this membrane module is made up of polyethylene hollow fiber microfiltration membrane, membrane pore size 0.1 μm, film silk internal diameter 0.27mm, film silk external diameter 0.42mm, membrane area 2m
2, an air bell is set below membrane module, plays water quality mixing effect.Membrane module has water pump pipe, and the water flowed out through going out water pump pipe meets emission standard.
6. utilize gram-bacteria to adsorb the method for set heavy metal ions in sewage as claimed in claim 1, it is characterized in that: the water ratio of the turbid liquid of the lower floor in drainage trap is lower than after 40%, take out membrane module, residue taken out and mixes with ceramic raw material, after shaping, fire ceramic tile.
7. utilize gram-bacteria to adsorb the method for set heavy metal ions in sewage as claimed in claim 6, it is characterized in that:
The water ratio of the turbid liquid of the lower floor in drainage trap is lower than after 40%, take out membrane module, residue is taken out, then by weight, get this residue 1 and sandstone 3, dolomite 4, high-calcium fly ass 1.5, phosphatic rock 12, asbestos 8, colemanite 0.8, quartz sand 30, nanometer silicon carbide fiber 4, starch 15, six ring stone (opal) 15, nano titanium oxide 0.5, alkali treatment modifying clay 20, stand-by;
The preparation method of described alkali treatment modifying clay is as follows: 1:3:2 takes kaolin, wilkinite, palygorskite clay in mass ratio, adds water to solid content about 30% and mixes, being placed in strong magnetic field circumstance and removing Ferromagnetic Impurities; The Ca (OH) of additional described one of clay raw materials gross weight 0.5 times again
2, mix, put into plumbago crucible and heat 2 hours at the temperature of 110 DEG C and use stainless steel stirring rod to stir, then be washed with distilled water to neutrality, dry, 300 DEG C of calcining 30min, pulverize, cross 240 mesh sieves, stand-by.
Above-mentioned raw materials is mixed, adds softening agent and binding agent, stir, then make the particle that particle diameter is 2mm.Then send into forming machine compression moulding under 80MPa, then the adobe suppressed being delivered in dry kiln and being dried to water content is 1.5%;
By the adobe of drying with the ramp to 1350 DEG C of 15 DEG C/min, insulation 2h, then be cooled to 880 DEG C with the speed of 5 DEG C/min, insulation 2h, 510 DEG C are cooled to again with the speed of 4 DEG C/min, insulation 1h, then with the ramp to 1160 DEG C of 8 DEG C/min, insulation 2.5h, 680 DEG C are cooled to again with the speed of 5 DEG C/min, insulation 1.5h, naturally cools to normal temperature, through edging, packs and obtain ceramic tile finished product.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109251373A (en) * | 2018-09-11 | 2019-01-22 | 山西山明环保科技有限公司 | Modified inorganic filler and preparation method thereof, composite material and preparation method |
| CN111715176A (en) * | 2020-05-18 | 2020-09-29 | 潘齐辉 | High-adsorption-rate ceramic material for absorbing heavy metal ions |
-
2015
- 2015-06-04 CN CN201510304659.0A patent/CN105152353B/en active Active
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| Title |
|---|
| 贾培,邓旭: "光合细菌处理重金属废水的研究进展", <<工业水处理>> * |
| 邹春艳，等: "细菌-矿物复合吸附剂对重金属离子的吸附与解吸作用", <<安全与环境学报>> * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109251373A (en) * | 2018-09-11 | 2019-01-22 | 山西山明环保科技有限公司 | Modified inorganic filler and preparation method thereof, composite material and preparation method |
| CN109251373B (en) * | 2018-09-11 | 2021-06-15 | 山西中恒工程有限公司 | Modified inorganic filler and preparation method thereof, and composite material and preparation method thereof |
| CN111715176A (en) * | 2020-05-18 | 2020-09-29 | 潘齐辉 | High-adsorption-rate ceramic material for absorbing heavy metal ions |
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| CN105152353B (en) | 2017-09-19 |
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Address after: 224000 Industrial Centralized Area of Xuefu Town, Yandu District, Yancheng City, Jiangsu Province Patentee after: JIANGSU WEALTH ENVIRONMENTAL ENGINEERING CO.,LTD. Address before: 224000 Industrial Centralized Area of Xuefu Town, Yandu District, Yancheng City, Jiangsu Province Patentee before: JIANGSU WEALTH ENVIRONMENTAL ENGINEERING Co.,Ltd. |
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